ライフサイエンスコーパス: TALEN

1 TALEN treatment efficiently disrupted E6 and E7 oncogene

2 TALEN were shown to induce mutations in the target codin

3 TALEN-Agouti mRNAs injected into zygotes of brown FvB x

4 TALEN-mediated gene editing is a useful tool for dissect

5 TALEN-mediated gene targeting in avian PGCs is therefore

6 TALEN-modified CMV-specific T cells retained specific ki

7 TALEN-modified Ossabaw swine fetal fibroblasts were effe

8 TALENs are important new tools for genome engineering.

9 TALENs targeting beta-catenin promoted endogenous HCC ca

10 TALENs thus appear to represent a highly facile platform

11 We used ICA to synthesize 20 TALENs of varying DNA target site length and tested thei

12 ew design guidelines for TALENs based on 205 TALENs tested, and established the scoring algorithm for

13 argeted changes in up to 33% (ZFNs) and 46% (TALENs) of blastocysts.

14 o combined both technologies and developed a TALEN-HDAdV hybrid vector, which significantly increased

15 Based on these findings, we engineered a TALEN variant that exhibits equal on-target cleavage act

16 We evaluated the ability of a TALEN pair to target the Ae. aegypti kmo gene, whose pro

17 nd flexible tool for designing highly active TALENs for genome-editing applications.

18 n, and we engineered a pair of highly active TALENs that induce modification of 54% of human beta-glo

19 We also developed highly active TALENs to human gamma-globin, a pharmacologic target in

20 Additionally, TALEN proteins are added to the repertoire of custom-des

21 ocyte polyploidy might be protective against TALEN-induced loss of heterozygosity, and indeed Apc gen

22 Using CRISPR-Cas9 and TALEN targeted human pluripotent stem cell clones, we pe

23 management aid of reagent concentrations and TALEN formulation.

24 t genome editing in T cells using CRISPR and TALEN approaches.

25 mia (AML) cell lines, and present CRISPR and TALEN based methods for accomplishing complete gene knoc

26 CRISPR and TALEN-based KO of Dck dramatically increased the IC(5)(0

27 e most widely used web tools for CRISPR- and TALEN-based genome editing.

28 ased program Mojo Hand for designing TAL and TALEN constructs for genome editing applications.

29 ed modularity and were active in TALE-TF and TALEN architectures.

30 describe a large-scale comparison of ZFN and TALEN mutagenicity in zebrafish.

31 sly been proposed to predict optimal ZFN and TALEN target sites did not predict mutagenicity in vivo.

32 The utility of CRISPR-Cas9 and TALENs for genome editing may be compromised by their of

33 a donor template along with CRISPR/Cas9 and TALENs respectively.

34 et binding of single-guide RNAs (sgRNAs) and TALENs.

35 CRISPR-Cas systems and TALENs can target desired genomic sites with high effici

36 ty in mammalian cells of custom TALE-TFs and TALENs using quantitative reverse-transcription PCR and

37 nd rapid construction of custom TALE-TFs and TALENs within 1 week and can be easily scaled up to cons

38 The modular architecture of ZFNs and TALENs allows for the rapid design of novel SSEs against

39 hlight the therapeutic potential of ZFNs and TALENs and discuss future prospects for the field, inclu

40 ZFNs and TALENs enable a broad range of genetic modifications by

41 The fact that ZFNs and TALENs have been used for genome modification of more th

42 Unlike ZFNs and TALENs that use protein motifs for DNA sequence recognit

43 characterize these cell lines, we anticipate TALEN-mediated genome editing of human cells becoming a

44 e efficiency, we administered adenoviral Apc TALENs and found that we could achieve a higher mutagene

45 A rate-limiting step in efficiently applying TALEN and CRISPR/Cas9 methods is the selection and desig

46 nome editing with designer nucleases such as TALEN and CRISPR/Cas enzymes has broad applications.

47 Sequence-specific nucleases such as TALEN and the CRISPR/Cas9 system have so far been used t

48 We also used FLASH to assemble TALENs for 96 endogenous human genes implicated in cance

49 We tested 48 FLASH-assembled TALEN pairs in a human cell-based EGFP reporter system a

50 thod and reagents for efficiently assembling TALEN constructs with custom repeat arrays.

51 SAPTA-based TALEN designs increased the average intracellular TALEN

52 d a significant negative correlation between TALEN mutagenicity and the number of CpG repeats in TALE

53 These results demonstrate that both TALEN and CRISPR-Cas9 are highly efficient tools for mod

54 we demonstrate for the first time that both TALEN and ZFN injected directly into pig zygotes can pro

55 Thus, cells transfected with both TALEN plasmids, a prerequisite for genomic editing, can

56 Both TALENs and CRISPR/Cas9 achieved gene targeting at simila

57 describe a mutant zebrafish line created by TALEN genome editing carrying a 23 bp deletion in gba1 (

58 re targeted to "spacer" sequences flanked by TALEN binding sites, larger deletions that extended beyo

59 onstructed an EYS-knockout zebrafish-line by TALEN technology which showed visual impairment at an ea

60 d a stable CERKL knockout zebrafish model by TALEN technology and a 7bp deletion in CERKL cDNA that c

61 C) technology and genome editing mediated by TALENs to generate isogenic subject-specific mutant and

62 16 of which were accessible and modified by TALENs in human cells.

63 The key features of direct mutagenesis by TALENs and oligodeoxynucleotides, minimal effort and hig

64 g the length of recognition DNA sequences by TALENs or ZFNs does not necessarily translate to a highe

65 fficient than cleavage of the same target by TALENs.

66 cific germline mutation in the mouse confirm TALEN mediated mutagenesis in the oocyte to be a viable

67 able high-throughput method for constructing TALENs has been published, and large-scale assessments o

68 off-target cleavage activity and corroborate TALENs as a highly specific platform for future clinical

69 r of using transcriptome analysis and CRISPR/TALEN-based KOs to identify and verify genes associated

70 nd enables scientists to more rapidly deploy TALENs for genome editing applications.

71 bly' method for rapid production of designer TALENs (dTALENs) that recognize unique DNA sequence up t

72 DMY-TALENs resulted in indel mutations at the targeted loci

73 een 13-20 bp and T nucleotide preceding each TALEN binding site) in zebrafish.

74 veloped a robust TALENs system in which each TALEN plasmid also encodes a fluorescence protein.

75 in immunoprecipitation (ChIP), gene editing (TALEN) and chromosome conformation capture (3C) to local

76 The most efficient TALEN was then selected for barley transformation.

77 Here, we describe a method that employs TALEN or CRISPR/Cas9-mediated knock-in of inducible degr

78 ly package full-length vector mRNAs encoding TALENs, but recombination during reverse transcription p

79 Here, we have engineered TALEN pairs targeting three human loci (CCR5, AAVS1 and

80 delivery system, we show that this enhanced TALEN toolkit has a high efficiency in inducing locus-sp

81 a simple transposon coselection strategy for TALEN-mediated gene modification in primary fibroblasts

82 enous gene-modification frequency of 39% for TALENs containing the repeat variable di-residue NK that

83 ines and developed new design guidelines for TALENs based on 205 TALENs tested, and established the s

84 target through linked protein domains (e.g. TALENs and zinc-finger nucleases).

85 orm is fully compatible with the Golden Gate TALEN and TAL Effector Kit 2.0, a widely used and effici

86 that the correction process did not generate TALEN-induced off targeting mutations by sequencing.

87 Using the beta-globin and gamma-globin TALENs, we generated cell lines that express GFP under t

88 Here, TALEN and CRISPR-Cas9, two versatile genome-editing tool

89 hat construction of one to two heterodimeric TALEN pairs for any given gene will, in most cases, enab

90 nduced by both homodimeric and heterodimeric TALENs are passed efficiently through the germline, in s

91 We also tested obligate heterodimeric TALENs and found that these nucleases induce mutations w

92 Here, we constructed homodimeric TALENs to 10 different targets in various endogenous zeb

93 However, TALENs targeting Apc were not as efficient in inducing i

94 Here, we assembled improved TALENs targeting the DMY gene and generated XY(DMY-) mut

95 Our improved TALENs system can be applied to all cultured cells to ac

96 LE-R activity and up to 10-fold reduction in TALEN activity compared with target sequences containing

97 the presence of multiple sequence repeats in TALEN genes hampers the use of lentiviral vectors.

98 utagenicity and the number of CpG repeats in TALEN target sites, suggesting that target site methylat

99 entification of TAL binding sites for use in TALEN design.

100 ed that mutations in the FokI domain used in TALENs to generate obligate heterodimeric complexes subs

101 The cleavage activity of individual TALENs was first tested in vivo using a yeast-based, sin

102 While the initial TALEN-design guidelines are very useful, user-friendly t

103 designs increased the average intracellular TALEN monomer activity by >3-fold, and resulted in an av

104 ely target almost any desired genomic locus, TALEN is a technology that can revolutionize the entire

105 nd DNA relatively independently; (ii) longer TALENs are more tolerant of mismatches yet are more spec

106 lity to target essentially any sequence make TALENs the superior technology for targeted mutagenesis

107 In parallel experiments, we employed mito-TALENs to induce breaks in distinct loci of the mitochon

108 ytes using mitochondria-targeted TALEN (mito-TALENs).

109 s, genotypes, gene expressions, morpholinos, TALENs, CRISPRs, antibodies, anatomical structures, mode

110 DMY-nanos3UTR-TALENs induced mutations were passed through the germlin

111 Neither CRISPR/Cas9 nor TALEN increased BAC transgenesis.

112 Notably, TALEN expression was overall marked by a low cytotoxicit

113 nscription activator-like effector nuclease (TALEN) and clustered regularly interspaced short palindr

114 nscription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) can be engineered

115 nscription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing tec

116 nscription activator-like effector nuclease (TALEN) binding sites in the genome using a new software

117 nscription Activator-Like Effector Nuclease (TALEN) exhibited few off-target effects and a low level

118 nscription activator-like effector nuclease (TALEN) is an artificial sequence-specific endonuclease t

119 nscription activator-like effector nuclease (TALEN) messenger RNA.

120 nscription activator-like effector nuclease (TALEN) mRNA allows highly efficient multiplex gene editi

121 nscription activator-like effector nuclease (TALEN) nuclease to knockdown endogenous Dot1L in Xenopus

122 nscription activator-like effector nuclease (TALEN) technologies are powerful strategies for the gene

123 nscription activator-like effector nuclease (TALEN) technology to only disrupt the expression of Jalp

124 tion activator-like (TAL) effector nuclease (TALEN)- and clustered regularly interspaced short palind

125 nscription activator-like effector nuclease (TALEN)-based universal correction of HBB mutations in si

126 nscription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cel

127 nscription activator-like effector nuclease (TALEN)-mediated genome editing, we created a panel of is

128 nscription activator-like effector nuclease (TALEN)-mediated genome engineering.

129 genic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs.

130 Tal-effector nucleases (TALEN) and clustered regularly interspaced short palindr

131 scription activator-like effector nucleases (TALEN) mRNAs into mouse zygotes transferred into foster

132 scription activator-like effector nucleases (TALEN) were designed against beta-catenin (Ctnnb1) and a

133 nscription factors (TALE-TFs) and nucleases (TALENs) using a hierarchical ligation procedure.

134 ion activator-like effector-based nucleases (TALENs).

135 scription activator-like effector nucleases (TALENs) ameliorates the R14del-associated disease phenot

136 scription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palind

137 ion activator-like (TAL) effector nucleases (TALENs) and clustered regularly interspaced short palind

138 scription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palind

139 scription activator-like effector nucleases (TALENs) and synthetic oligodeoxynucleotides into one-cel

140 scription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short pa

141 scription activator-like effector nucleases (TALENs) are a new class of engineered nucleases that are

142 TAL-effector nucleases (TALENs) are attractive tools for sequence-specific genom

143 Tal-effector nucleases (TALENs) are engineered proteins that can stimulate preci

144 scription activator-like effector nucleases (TALENs) are powerful new research tools that enable targ

145 scription activator-like effector nucleases (TALENs) are programmable nucleases that join FokI endonu

146 scription activator-like effector nucleases (TALENs) can be designed to cleave chosen DNA sequences,

147 scription activator-like effector nucleases (TALENs) comprise a powerful class of tools that are rede

148 scription Activator-Like Effector Nucleases (TALENs) consist of a nuclease domain fused to a DNA bind

149 scription activator-like effector nucleases (TALENs) enable genome engineering in cell culture and ma

150 scription activator-like effector nucleases (TALENs) enable targeted mutagenesis in a variety of orga

151 scription activator-like effector nucleases (TALENs) for five distinct genomic loci.

152 scription activator-like effector nucleases (TALENs) for genome engineering, we demonstrate efficient

153 scription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and

154 scription Activator-Like Effector Nucleases (TALENs) has provided a new experimental partnership for

155 scription activator-like effector nucleases (TALENs) have become a powerful tool for genome editing d

156 nucleases (ZFNs) and TAL effector nucleases (TALENs) have been shown to induce targeted mutations, bu

157 scription activator-like effector nucleases (TALENs) have emerged as a highly effective tool for geno

158 scription activator-like effector nucleases (TALENs) have shown promise as facile and broadly applica

159 scription activator-like effector nucleases (TALENs) induces apoptosis, inhibits growth, and reduces

160 scription activator-like effector nucleases (TALENs) is a valuable tool for precise, site-specific ge

161 scription activator-like effector nucleases (TALENs) or clustered, regularly interspaced, short palin

162 scription activator-like effector nucleases (TALENs) or RNA-guided clustered regularly interspaced sh

163 scription activator-like effector nucleases (TALENs) or zinc-finger nucleases (ZFNs).

164 scription activator-like effector nucleases (TALENs) provide a powerful new approach for targeted zeb

165 scription activator-like effector nucleases (TALENs) provide powerful platforms for genome editing in

166 scription activator-like effector nucleases (TALENs) represent a promising approach for targeted knoc

167 scription activator-like effector nucleases (TALENs) that are based on bacterial TALEs fused to the F

168 scription activator-like effector nucleases (TALENs) that recognize two adjacent unique DNA sequences

169 on enzyme to produce TAL effector nucleases (TALENs) that, in pairs, bind adjacent DNA target sites a

170 scription activator-like effector nucleases (TALENs) to completely eradicate all LEDGF/p75 expression

171 scription activator-like effector nucleases (TALENs) to localize to mitochondria and cleave different

172 scription activator-like effector nucleases (TALENs) to target interleukin-2 receptor subunit gamma (

173 scription activator-like effector nucleases (TALENs) with DNA oligonucleotides (ODNs).

174 er nucleases (ZFNs), TAL effector nucleases (TALENs), and CRISPR-associated system 9 (Cas9) proteins,

175 scription activator-like effector nucleases (TALENs), and CRISPR/Cas RNA-guided nucleases (RGNs).

176 scription activator-like effector nucleases (TALENs), completely removes ORN sensitivity to bombykol

177 nucleases (ZFNs) and TAL effector nucleases (TALENs), have made it possible to precisely modify plant

178 scription activator-like effector nucleases (TALENs), relies on safe and effective means of deliverin

179 cription Activator-Like Effectors Nucleases (TALENs) and Cas9-gRNA allows genetic modifications to be

180 cription activator-like effectors nucleases (TALENs) with broadly improved DNA cleavage specificity,

181 anscription factors (TALE-TFs) or nucleases (TALENs), enabling precise gene manipulations.

182 TALE nucleases (TALENs) have been used with great success in a number of

183 In this work we use TALE nucleases (TALENs) to target a reporter construct to the DDX4 (vasa

184 LE recombinases (TALE-R) and TALE nucleases (TALENs) with each DNA base at this position.

185 finger nucleases (ZFNs) and Tale nucleases (TALENs), and has enabled us to insert a 15-kb inducible

186 TALE nucleases (TALENs), generated by fusing the FokI cleavage domain to

187 e (TAL) effector domains fused to nucleases (TALENs) demonstrate enormous potential for genome editin

188 ion activator-like [TAL] effector nucleases [TALENs], and homing endonucleases [also called meganucle

189 ese results demonstrate the applicability of TALEN-mediated genome editing to a scalable process, whi

190 The assembly of TALEN constructs, is also simplified by using the TAL-si

191 Zygotic coinjection of TALEN mRNAs directed to the Agouti, miR-205, and the Arf

192 tabase searches, we designed a collection of TALEN constructs to knockout 88 human genes that are ass

193 argeting vector influenced the efficiency of TALEN-mediated homologous recombination.

194 We found little evidence of TALEN off-target effects, but each clonal line neverthel

195 Cytoplasmic injections of TALEN mRNAs into livestock zygotes were capable of induc

196 ng targeted mutagenesis by microinjection of TALEN mRNA within the mouse oocyte.

197 report detailed genotypes and phenotypes of TALEN-edited MECP2 mutant cynomolgus monkeys serving as

198 Our results establish the robustness of TALEN technology and demonstrate that FLASH facilitates

199 The primary advantage of TALENs over other sequence-specific nucleases, namely zi

200 in hPSCs with a focus on the application of TALENs and CRISPR/Cas9.

201 effective method for large-scale assembly of TALENs.

202 Effective design of TALENs requires a combination of selecting appropriate g

203 m and demonstrate controlled co-injection of TALENs or CRISPR/Cas9 together with donor template into

204 tured cells, two plasmids encoding a pair of TALENs are co-transfected, followed by limited dilution

205 A pair of TALENs binds to two DNA recognition sites separated by a

206 Combining the high specificity of TALENs with efficient lentiviral gene delivery should ad

207 that one subunit of ZFNs and one subunit of TALENs can form a pair of hybrid nucleases with expanded

208 smission data from CRISPR/Cas9 with those of TALENs and ZFNs and shows that efficiency of CRISPR/Cas9

209 entiviral-vector RNA would allow transfer of TALENs as mRNA.

210 We report here the use of TALENs to rapidly and efficiently generate mutant allele

211 useful, user-friendly tools defining optimal TALEN designs for robust genome editing need to be devel

212 sites, facilitating the selection of optimal TALEN pairs based on predicted activity.

213 eering technologies-piggyBac, CRISPR/Cas9 or TALEN.

214 iRNAs were not detected from CRISPR/Cas9- or TALEN-induced DSBs within the examined endogenous genes

215 Here, we used CRISPR/Cas9- or TALEN-triggered DSBs to characterize diRNAs in Arabidops

216 HTGTS with different Cas9:sgRNA or TALEN nucleases revealed off-target hotspot numbers for

217 ntegrases at the H11 locus by spontaneous or TALEN-assisted homologous recombination.

218 ondria-targeted restriction endonucleases or TALENs.

219 ins, which are intrinsically cell-permeable; TALEN proteins, which can be internalized via conjugatio

220 rest, SAPTA gives a ranked list of potential TALEN target sites, facilitating the selection of optima

221 blished the scoring algorithm for predicting TALEN activity (SAPTA) as a new online design tool.

222 ssive DNA-binding energy can lead to reduced TALEN specificity in cells.

223 Remarkably, TALENs or CRISPR-Cas9-mediated double-strand DNA breaks

224 Here, we report TALEN-mediated genome editing of the IL2RG locus.

225 We have developed a robust TALENs system in which each TALEN plasmid also encodes a

226 be designed to cleave chosen DNA sequences, TALENs have activity against related off-target sequence

227 Here we report the assembly of several TALENs for a specific genomic locus in barley.

228 gene in the first coding exon with a single TALEN pair yielded trace LEDGF/p75 levels that were viro

229 DDX4 locus were also created using a single TALEN pair.

230 Coselection after treatment with a single TALEN-pair enabled isolation of colonies with mono- and

231 on may explain the poor mutagenicity of some TALENs in vivo.

232 to the advent of the versatile and specific TALEN systems, and most recently the highly accessible C

233 The rational design of a CCR5-specific TALEN pair decreased off-target activity at the closely

234 ciple, we transferred CCR5- and TCR-specific TALEN pairs for efficient targeted genome editing and ab

235 ff-target sites for CCR5- and AAVS1-specific TALENs.

236 and (4) output files designed for subsequent TALEN construction using the Golden Gate assembly method

237 Here we report successful TALEN-mediated mutagenesis of an X-linked, Rett syndrome

238 ludes engineered zinc finger proteins, TALEs/TALENs, and the CRISPR/Cas9 system as nucleases for geno

239 ammalian oocytes using mitochondria-targeted TALEN (mito-TALENs).

240 Mitochondria-targeted TALEN (mitoTALEN) expression led to permanent reductions

241 ct cervical application of HPV16-E7-targeted TALENs effectively mutated the E7 oncogene, reduced vira

242 Microinjection of MECP2-targeting TALEN plasmids into rhesus and cynomolgus zygotes leads

243 ) and found that, with beta-globin-targeting TALENs, similar levels of on- and off-target activity in

244 ese methods to identify, construct, and test TALENs that were used with HDR donors in hESCs to genera

245 All 14 tested TALEN pairs (100%) introduced small insertions and delet

246 We conclude that TALEN-mediated mutagenesis can be an effective tool for

247 We observed that TALEN and ZFN have a reduced capability of secondary hom

248 rescence in situ hybridization revealed that TALEN pairs targeting the Agouti locus induced site-dire

249 Here we show that TALEN of dmrt1 efficiently induced mutations of this gen

250 s of the resulting transformants showed that TALEN-induced double strand breaks led to the introducti

251 We conclude that TALENs are highly active in the Ae. aegypti germline, an

252 Using deep sequencing, we found that TALENs are significantly more likely to be mutagenic and

253 In contrast, we found that TALENs could easily be manufactured and that over half (

254 ed nucleotides, raising the possibility that TALENs might be used to perform allele-specific gene dis

255 We show that TALENs mediate homology-directed repair of the DDX4 locu

256 Recent work has shown that TALENs can induce mutations in endogenous zebrafish gene

257 Our data suggest that TALENs employing the specific architectures described he

258 her, the results from our study suggest that TALENs have potential as a therapeutic strategy for HPV

259 The TALEN pairs were designed to induce double-strand DNA br

260 oftware program, TALENSeek, (2) assemble the TALEN genes by combining golden gate cloning with modifi

261 s, larger deletions that extended beyond the TALEN-binding sequences were also detected and were simi

262 Furthermore, endowing the TALEN-engineered cells with a CD19 CAR led to efficient

263 en made possible with the development of the TALEN and CRISPR/Cas9 methods.

264 Here we report the utilization of the TALEN and CRISPR/Cas9 systems to induce targeted mutatio

265 We used two of the TALEN pairs to mutate HPRT1 in human cells and ADH1 in A

266 To illustrate the utility of the TALEN-mediated knockout technique, 6 individual genes (T

267 ts from the FLASH protocol, and (3) test the TALEN pairs in an amplification-based HDR assay that is

268 We show that the TALEN was extremely efficient in mutating Dot1L when exp

269 two newly developed gene-editing tools, the TALEN and CRISPR/Cas systems, we have developed a genome

270 The TALENs showed comparable activity to benchmark ZFNs, wit

271 We asked whether these TALENs could create targeted somatic mutations after hyd

272 We use this TALEN-mediated editing approach to develop a process for

273 id sequences of their DNA-binding domains to TALEN nucleotide targets.

274 We transfected TALENs along with a targeting vector into Jurkat cells,

275 Coselection after treatment with two TALEN-pairs directed against the same chromosome enabled

276 MiPs from dystrophic dogs that had undergone TALEN-mediated correction of the MD-associated mutation

277 To better understand TALEN specificity, we profiled 30 unique TALENs with dif

278 and TALEN specificity, we profiled 30 unique TALENs with different target sites, array length and dom

279 With this updated TALEN system, we successfully used single-stranded DNA o

280 Here, we used TALEN and CRISPR/Cas-mediated gene editing and hPSC-dire

281 nd reduce CPI-associated toxicities, we used TALEN technology to render tumor-reactive T cells resist

282 We used TALEN-mediated genome editing in fertilized mouse oocyte

283 We used TALEN-technology to knock out the nuclear gene encoding

284 erimentally useful human cell lines, we used TALENs to definitively eradicate LEDGF/p75 by deleting e

285 We used TALENs to generate five zebrafish abcd1 mutant allele li

286 cking all four zebrafish Mesp genes by using TALEN-mediated genome editing.

287 e generated a zyx mutant in Drosophila using TALEN endonucleases and used this to show that Zyx antag

288 s in pig, goat, and cattle fibroblasts using TALEN mRNA and oligonucleotide transfection with efficie

289 labeled vimentin with an mEmerald tag using TALEN genome editing.

290 nerated genetic mosaic adult zebrafish using TALEN genome editing and demonstrate somatic inactivatio

291 brafish (zap70(y442)) that was created using TALENs.

292 ptimized procedure for genomic editing using TALENs is also presented.

293 In the present study, we utilized TALEN- and CRISPR/Cas9-induced mutations to analyze the

294 These studies expand the realm of verified TALEN activity from cultured human cells to an intact eu

295 Sequence-verified TALENs or TALE-TF plasmids targeting 17 bp target sequen

296 set was cleaved in a yeast-based assay with TALEN pairs constructed with our reagents.

297 11-33% of 1-cell stage embryos injected with TALEN mRNAs targeting rb1 exon 2 or 3 develop tumors beg

298 targeting occurred at an average of 14% with TALENs and 33% with CRISPR.

299 Here we used designer nucleases (ZFNs, TALENs, and CRISPR/Cas9) to introduce DSBs on two chromo

300 We observed improved efficiency using ZFNs, TALENs, two CRISPR/Cas9, and CRISPR/Cas9 nickase to targ