ライフサイエンスコーパス: TAP1

1 s, and the ER antigen peptide transporter 1 (TAP1).

2 nsporter associated with antigen processing (TAP1).

3 rate that US6 inhibits the binding of ATP by TAP1.

4 y the cytosolic nucleotide binding domain of TAP1.

5 for nucleotide binding relative to wild type TAP1.

6 in C57BL/6 mice with a targeted deletion in TAP1.

7 ing TAP1 and cooperates with p53 to activate TAP1.

8 biquitination and proteasomal degradation of TAP1.

9 control groups, we observed a difference in TAP1.

10 complex, diffuse slowly, as does GFP-tagged TAP1.

11 AP-deficient cells transfected with only rat TAP1.

12 essential to achieve full stability of human TAP1.

13 s in a stop codon in the catalytic domain of TAP1.

14 SA occurred in TAP1(0) and beta2m(0)/class I-deficient mice with a mixe

15 nsporter associated with antigen processing (TAP1(0)) on a B6,129 background, and HLA-B27-transgenic

16 ncy of alleles for peptide transporter genes TAP1 (0101) and TAP2 (0101) genes as well as tumor necro

17 protein ICP47 to block peptide transport by TAP1/2 and examined the effects of TAP blockade on the p

18 The TAP1/2 complex translocates peptides from the cytosol in

19 s into the endoplasmic reticulum (ER) by the TAP1/2 complex.

20 was evaluated at multilevel in TAP1/2iso and TAP1/2 cotransfected T2 cells, a mutant cell line deplet

21 and LMP7, shark-specific LMP7-like, and the TAP1/2 genes are linked to class I/II.

22 oteolysis, env peptides are retransported by TAP1/2 into the ER, where association with class I occur

23 Studies using TAP1/2-deficient mice and Ag presentation inhibitors ind

24 e cytosol, we analyzed the processing of two TAP1/2-dependent epitopes containing N-linked glycosylat

25 ic CD8+ CTL, the processing pathway required TAP1/2-mediated transport of cytosolic peptides into the

26 on of TAP2iso was evaluated at multilevel in TAP1/2iso and TAP1/2 cotransfected T2 cells, a mutant ce

27 Of the patients with polymorphic TAP1, 36% were positive for HLA-DRB1*0102 (P=.021; P=.14

28 asured by coimmunoisolation of Kb using anti-TAP1 Abs, while only 20% of the Kb heavy chain molecules

29 The reduction in TAP1 abundance decreased peptide antigen translocation i

30 DRB1*1501-DQA1*0102-DQB1*0602 haplotype, the TAP1 allele encoding Ile at residue 333, and the DRB5-01

31 restored after transfection of a functional TAP1 allele.

32 ur findings indicate that mK3 interacts with TAP1 and -2 via their C-terminal domains and with class

33 ation was driven by CpGs associated with the TAP1 and ALDH3A1 genes, findings that were validated in

34 Two previously undescribed antiviral ISGs, TAP1 and BMP2, were further validated.

35 is homologous to p53, is capable of inducing TAP1 and cooperates with p53 to activate TAP1.

36 T. denticola tap1 and flanking DNA were identified, cloned, and seque

37 tively increased the amount of ubiquitinated TAP1 and increased its degradation in the proteasome of

38 ent in the core transmembrane (TM) domain of TAP1 and is used only by the unassembled subunits.

39 The expression of TAP1 and LMP2 are both greatly reduced in IRF-1-deficien

40 E that is essential for the up-regulation of TAP1 and LMP2 by IFN gamma.

41 However, overexpression of Jak1 did increase TAP1 and LMP2 expression independent of IFN-gamma, indic

42 The effects of IFN-gamma on TAP1 and LMP2 expression revealed a loss of up-regulatio

43 these transactivators regulate differential TAP1 and LMP2 gene transcription is not known.

44 The TAP1 and LMP2 genes are central for class I MHC function

45 The TAP1 and LMP2 genes are transcribed from a shared bidire

46 with luciferase reporter constructs for both Tap1 and Lmp2 genes.

47 These findings suggest that the loss of TAP1 and LMP2 induction is a defect in the earliest step

48 Northern blot revealed reduced amounts of Tap1 and Lmp2 mRNA in NOD mice, and 5'-rapid amplificati

49 The role of IRF-1 in the regulation of TAP1 and LMP2 suggests a mechanism for the antiviral pro

50 ported to have abnormally low expressions of TAP1 and LMP2.

51 ular mechanism of IFN gamma up-regulation of TAP1 and LMP2.

52 ly, a lack of association between alleles of TAP1 and TAP2 (approximately 15 kb) has been observed, s

53 rate that the dimerization interface between TAP1 and TAP2 and the tapasin docking sites for PLC asse

54 We show tapasin binding to both TAP1 and TAP2 and to the corresponding nucleotide bindin

55 cal and functional evidence that the NBDs of TAP1 and TAP2 are non-equivalent.

56 Here we show that both TAP1 and TAP2 are phosphorylated under physiological con

57 Thus, the MSRs of both TAP1 and TAP2 are required for binding peptide.

58 he extra N-terminal domain TMD0 of mammalian TAP1 and TAP2 as well as avian TAP2 recruits tapasin.

59 ased upon cotransfection with genes encoding TAP1 and TAP2 but not individual TAP subunits, beta(2)m,

60 TAP1 and TAP2 contain an N-terminal hydrophobic membrane

61 We found that avian and mammalian TAP1 and TAP2 form heterodimeric complexes across taxa.

62 Strikingly, however, only TAP1 and TAP2 from the same taxon can form a functional

63 We have examined the polymorphism in mouse TAP1 and TAP2 in inbred mice.

64 Differences between TAP1 and TAP2 in the nucleotide-binding site may be rela

65 nhibited photocrosslinking of 8-azido-ATP to TAP1 and TAP2 it seems that ICP47 does not prevent ATP f

66 lass I heavy chain, beta2 microglobulin, and TAP1 and TAP2 mRNAs revealed increased expression in a m

67 expressed as individual subunits or domains, TAP1 and TAP2 NBD differ markedly in their nucleotide bi

68 st the possibility of distinct functions for TAP1 and TAP2 NBD during a single translocation cycle.

69 TAP is made up of the TAP1 and TAP2 polypeptides, which each possess a nucleot

70 ER by an MHC-encoded transporter composed of TAP1 and TAP2 protein delivery.

71 TAP1 and TAP2 proteins are also induced rapidly, increas

72 The TAP1 and TAP2 proteins form a heterodimer that transport

73 as deduced from differential labeling of the TAP1 and TAP2 subunits using sICP47 fragments with chemi

74 For these studies, we tagged the TAP1 and TAP2 subunits with enhanced cyan fluorescent pr

75 two nucleotide-binding domains (NBD) in the TAP1 and TAP2 subunits.

76 ey lysine residues in the Walker A motifs of TAP1 and TAP2 suggests that TAP1-mediated ATP hydrolysis

77 associated with antigen-processing subunits TAP1 and TAP2 that were altered at the conserved lysine

78 and Psmb9 and the antigen transporter genes Tap1 and Tap2 The PSMB8 inhibitor ONX-0914 reversed the

79 nsporter associated with antigen processing (TAP1 and TAP2), members of the ABC transporter family th

80 er associated with antigen processing genes (TAP1 and TAP2).

81 Two subunits, TAP1 and TAP2, are necessary and sufficient for peptide

82 Two subunits, TAP1 and TAP2, are required for peptide transport, and A

83 nsporters associated with antigen processing TAP1 and TAP2, DMA and DMB which are involved in editing

84 gen processing (TAP) comprises two subunits, TAP1 and TAP2, each containing a hydrophobic membrane-sp

85 sporter consists of two homologous subunits, TAP1 and TAP2, each of which contains an N-terminal doma

86 s an ABC transporter formed of two subunits, TAP1 and TAP2, each of which has an N-terminal membrane-

87 tructs except T1ctr interacts with wild type TAP1 and TAP2, indicating possibilities for homodimeriza

88 lved in antigen processing and presentation (TAP1 and TAP2, MHC class I and II, CD80), regulators of

89 cating possibilities for homodimerization of TAP1 and TAP2, or of oligomerization of TAP1/TAP2 hetero

90 comprises two structurally related subunits, TAP1 and TAP2, that form stable complexes in endoplasmic

91 by the association of two half-transporters, TAP1 and TAP2, with a typical ABC transporter core that

92 ry (APM), including LMP2 and LMP7 as well as TAP1 and TAP2.

93 aining just the membrane-spanning regions of TAP1 and TAP2.

94 The TAP transporter is a heterodimer of TAP1 and TAP2.

95 ich are located in the N-terminal domains of TAP1 and TAP2.

96 in the presence of 2 potent TACE inhibitors: TAP1 and TMI-1.

97 lecule, which recruits one MHC I molecule to TAP1 and/or TAP2.

98 ssociated with antigen processing subunit 1 (TAP1) and a proteasome subunit, low molecular weight pro

99 esentation, including beta-2 microglobulin, (TAP1), and TAP2.

100 s in fetal thymus organ cultures (FTOC) from TAP1+ and TAP1- mice.

101 TCR-delta, TAP1, and IL-17RA deficiency specifically affects early

102 in, protein disulfide isomerase A3, tapasin, TAP1, and TAP2.

103 ll line had little or no expression of LMP2, TAP1, and tapasin, critical components of the HLA class

104 Lmp2 and Tap1 are genes located in the MHC class II region, and t

105 curring Asp(668) and Gln(701) alterations of TAP1 are likely to contribute to attenuated catalytic ac

106 Alterations of TAP1 Asp(668) alone or in combination with TAP1 Gln(701)

107 tection is not affected by TCR-delta, MHCII, TAP1, B cell, IL-17RA, or IL-12p35 deficiency, suggestin

108 eeding the mice to (C57BL/6 x B10.D2)F(1) or TAP1(+/-) backgrounds partially restored cytotoxic activ

109 We also report that mouse TAP1 begins 172 amino acids upstream of the previously p

110 e constitutive and TNF-induced expression of TAP1 but are not necessary for the IFN-gamma response.

111 ssociated with antigen processing deficient (TAP1(-/-)), CD8alpha(-/-), perforin(-/-), and CD1d(-/-)

112 Transfection with TAP1 cDNA restores TAP1 protein abundance, HLA class I b

113 L is restored by transfection with wild-type TAP1 cDNA.

114 Incubation of tapasin(-/-) or TAP1(-/-) cells at 26 degrees C decreased susceptibility

115 Alternate MHC-I processing by TAP1-/- cells was enhanced by preincubation at 26 degree

116 recombinational site centromeric to the Lmp2/Tap1 complex by breeding the two strains.

117 cture of the C-terminal ABC ATPase domain of TAP1 (cTAP1) bound to ADP.

118 porter associated with antigen processing 1 (Tap1)-deficient mice with reduced cell surface expressio

119 ass I biosynthesis similar to those found in TAP1-deficient cell lines.

120 TAP1-deficient fibroblasts were more susceptible to VSV

121 In TAP1-deficient mice, both forms undergo carbohydrate pro

122 r hook protein (FlgE) was synthesized in the Tap1-deficient mutant; however, electron microscopy reve

123 s into the T. denticola chromosome, creating Tap1-deficient mutants.

124 ndoplasmic reticulum-golgi transport and was TAP1 dependent.

125 Ag-processing pathway is both proteasome and TAP1 dependent.

126 the erythromycin resistance cassette within tap1 did not terminate fla operon transcription in eithe

127 that of human and mice: DPB/DPA, Ring3, DMB, TAP1, DOB, DRB2, DRA3, DRB1, DRA2, and DRA1.

128 T cells were observed in mice deficient for TAP1 due to TEIPP antigen presentation on all body cells

129 Complex formation with TAP1 enhanced the binding affinity of the TAP2 nucleotid

130 cells was found to be associated with a low TAP1 expression and a reduced function of peptide transp

131 are defective in IFN-gamma-induced LMP2 and TAP1 expression, loss of which inhibits presentation of

132 l lines with distinct phenotypes of LMP2 and TAP1 expression.

133 P14 TAP1- FTOC and negative selection in P14 TAP1+ FTOC.

134 positive selection of anti-LCMV CTLs in P14 TAP1- FTOC and negative selection in P14 TAP1+ FTOC.

135 The flexibility in the regulation of the TAP1 gene may reflect its role in maintaining immune sur

136 Because of the proximity of the TAP1 gene to human leukocyte antigen (HLA) class II gene

137 that the peptide transporter encoded by the Tap1 gene within H2g7 is defective, and this contributes

138 fficient for regulating transcription of the TAP1 gene, binding of both factors is required for LMP2

139 hared bidirectional promoter of the Lmp2 and Tap1 genes in the nonobese diabetic (NOD) mouse.

140 llelic exchange incorporated the interrupted tap1 genes into the T. denticola chromosome, creating Ta

141 f TAP1 Asp(668) alone or in combination with TAP1 Gln(701) had only small effects on TAP activity.

142 Computer analysis indicated that Tap1 had no significant membrane spanning regions, sugge

143 s; however, the initial gene of this operon, tap1, has no known function.

144 es share a bi-directional promoter, LMP2 and TAP1 have differential cellular expression.

145 Preferential ATP interaction with TAP1, if occurring in vivo, might polarize the transport

146 a putative promoter, preceding T. phagedenis tap1 in a region of divergent transcription.

147 including Asp(668) in the Walker B region of TAP1 (in place of a highly conserved glutamic acid), and

148 acid), and Gln(701) in the switch region of TAP1 (in place of a highly conserved histidine).

149 t with RRP might be determined by sequencing TAP1, in conjunction with HLA class II genes.

150 nsporter associated with antigen processing (TAP1)-independent but sensitive to inclusion of inhibito

151 the transport cycle such that ATP binding to TAP1 initiates the cycle.

152 porter associated with antigen processing-1 (TAP1), interferon regulatory factor-1 (IRF1), and class

153 is cassette, a second plasmid that contained tap1 interrupted with a modified erythromycin resistance

154 uenced, and a suicide plasmid that contained tap1 interrupted with an erythromycin resistance cassett

155 used by IFN gamma, but still fails to induce TAP1, IRF1, or class I MHC molecules.

156 We propose that treponemal Tap1 is analogous to FliK, which is involved in monitori

157 TAP1 is expressed constitutively.

158 results indicate that nucleotide binding to TAP1 is not a requirement for peptide binding to TAP com

159 Since the mutant TAP1 is significantly impaired for nucleotide binding, t

160 Here we found that TAP1 is strongly induced by p53 and DNA-damaging agents

161 Transporter associated with Ag processing-1 (TAP1) is induced by IFN-gamma more rapidly than is HLA c

162 Mutants TAP1(K544M) and TAP2(K509M) were expressed in insect cel

163 levels of translocation are detectable with TAP1(K544M).TAP2 complexes.

164 ecordings of hippocampal neurons from beta2m/TAP1 knockout (KO) mice, which have reduced MHCI surface

165 Both TAP1 knockout and wild-type DCs showed enhanced cross-pr

166 C class II, beta2-microglobulin (beta2m) and TAP1 knockout mice, the V beta 4-specific T cell stimula

167 TAP1-knockout macrophages were incubated overnight with

168 omplexes also demonstrate that the extent of TAP1 labeling is dependent upon the presence of a functi

169 The Tap1-Lmp2 promoter from NOD mice showed reduced transcri

170 hain promoter activity, while repressing the TAP1/LMP2 promoter.

171 otide polymorphisms (SNPs) in 5 genes (LMP2, TAP1, LMP7, TAP2, and Tapasin) were investigated for ass

172 Tapasin(-/-) and TAP1(-/-) macrophages had decreased MHC-I stability and

173 cells and experiments with tapasin(-/-) and TAP1(-/-) macrophages that characterize alternate MHC-I

174 sing was diminished in both tapasin(-/-) and TAP1(-/-) macrophages.

175 TAP1-/- macrophages exhibited decreases in cell surface

176 lker A motifs of TAP1 and TAP2 suggests that TAP1-mediated ATP hydrolysis is not essential for peptid

177 e strikingly similar to those in beta2 m(-/-)TAP1(-/-) mice, which lack cell surface expression of al

178 nthesized by recombinant vaccinia viruses in TAP1(-/-) mice.

179 thymus organ cultures (FTOC) from TAP1+ and TAP1- mice.

180 er, bone marrow-derived dendritic cells from TAP1-/- mice showed increased antigen presentation by CD

181 Consistent with the above results, TAP1-/- mice were found to have a higher percentage of t

182 The human TAP1 mRNA 3'-UTR contains a 6-mer canonical seeding site

183 the ER stress-associated reduction in human TAP1 mRNA and protein levels could be reversed with an m

184 s for miR-346, we demonstrate that the human TAP1 mRNA is a direct target of miR-346.

185 Altered psmb9, SGK, and Tap1 mRNA levels were also observed in an in vivo model

186 recruitment correlates with a rise in mature TAP1 mRNA.

187 omplex, we generated two chimeras containing TAP1 MSR and TAP2 NBD (T1MT2C) or TAP2 MSR and TAP1 NBD

188 P1 MSR and TAP2 NBD (T1MT2C) or TAP2 MSR and TAP1 NBD (T2MT1C).

189 exes correlates with enhanced binding of the TAP1 NBD-containing constructs to ATP-agarose beads.

190 Inoculation of C57BL/6 mice with TAP1-negative cells produced large and persistent tumors

191 BL/6 mice with mixtures of TAP1-positive and TAP1-negative cells produced tumors composed exclusively

192 Both TAP1-positive and TAP1-negative cells produced tumors in athymic mice, con

193 ells produced tumors composed exclusively of TAP1-negative cells, indicating in vivo selection for ce

194 ression, matched panels of TAP1-positive and TAP1-negative tumor cell lines were generated from a par

195 ient mice, although not in mice deficient in TAP1 or MHC class II expression.

196 NAs are induced rapidly, increasing 20-fold (TAP1) or 10-fold (TAP2) by 12 h, whereas HLA class I mRN

197 porter associated with antigen processing 1 (TAP1) or TAP2.

198 related genes such as beta(2)-microglobulin, Tap1, or Lmp2, but did not affect MHC class II levels.

199 nd an inverted CAAT-like box in the Lmp2 and Tap1 orientations, respectively.

200 Furthermore, we found that by inducing TAP1, p53 enhances the transport of MHC class I peptides

201 tivation of a subset of genes, including the TAP1 peptide transporter and proteasome subunit beta typ

202 FN-gamma and interleukin-6 (IL-6) but not on TAP1, perforin, IL-4, Fas ligand, or inducible nitric ox

203 explain the experimental data available for TAP1-Phe-265.

204 We examined whether a known TAP1 polymorphism in the ATPase domain altered the sever

205 Inoculation of C57BL/6 mice with mixtures of TAP1-positive and TAP1-negative cells produced tumors co

206 Both TAP1-positive and TAP1-negative cells produced tumors in

207 ects in tumor progression, matched panels of TAP1-positive and TAP1-negative tumor cell lines were ge

208 In contrast, TAP1-positive cells did not generate lasting tumors, alt

209 The regulatory elements within the LMP2/TAP1 promoter and the transcription factors that bind th

210 A gamma-activating sequence (GAS) in the TAP1 promoter is necessary for the rapid induction by IF

211 ectants reveals that IFN-gamma activates the TAP1 promoter more rapidly than the HLA-B7 class I promo

212 f268 reduced the activity of psmb9, SGK, and Tap1 promoter-reporter constructs.

213 porter associated with antigen processing-1 (TAP1) promoter was compared in HeLa cells and endothelia

214 how that culture in tobacco extracts reduces TAP1 protein abundance and membrane HLA class I levels.

215 Culture in tobacco extracts reduces TAP1 protein abundance, but not steady-state mRNA abunda

216 Transfection with TAP1 cDNA restores TAP1 protein abundance, HLA class I biosynthesis, and ce

217 The reduction of TAP1 protein occurs within 4 h and is dose-dependent.

218 ut not extracts of other substances, reduces TAP1 protein, but does not reduce expression of HLA clas

219 genes enriched in immune response processes (TAP1, PSMB8, PSMB9, HLA-DQB1, HLA-DQB2, HLA-DMA, and HLA

220 complex (MHC), at 6p21.33, association with TAP1-PSMB8 (rs3819721, P=5.2E-06) seems to derive from l

221 of tapasin binding to the core TM domain of TAP1 single chains is mysterious because this interactio

222 ty at the first nucleotide binding site (the TAP1 site) of TAP complexes.

223 The mutant TAP1 subunit is significantly impaired for nucleotide bi

224 porter subunit TAP2 and reduce levels of the TAP1 subunit, MHC class I molecules, and EBNA1, a protei

225 A-S microsomes associated with the remaining TAP1 subunit.

226 ediated by two C-terminal fragments of human TAP1 (T1c, residues 452-748 and T1ctr, residues 472-748)

227 By contrast, TAP1/T1MT2C and TAP2/T2MT1C complexes, although observed

228 The enhanced translocation efficiency of TAP1/T2MT1C relative to TAP2/T1MT2C complexes correlates

229 The NBD-switched complexes, T1MT2C/T2MT1C, TAP1/T2MT1C, and TAP2/T1MT2C, all translocate peptides,

230 We show that TAP1/T2MT1C, TAP2/T1MT2C, and T1MT2C/T2MT1C complexes bi

231 <0.05) of CD11c, CD40, CCR7 as well as LMP2, TAP1, TAP2 and tapasin than conv. mix-matured DC.

232 sociated with antigen processing (TAP) loci (TAP1, TAP2) were investigated in 100 members of 16 famil

233 inducible APM components low-m.w. protein 2, TAP1, TAP2, and tapasin.

234 = 0.001) up-regulation of the APM components TAP1, TAP2, and tapasin.

235 on of the four genes encoded within the MHC (TAP1, TAP2, LMP2, and LMP7), as well as LMP10, which is

236 molecular weight TAP complexes that contain TAP1, TAP2, tapasin, and class I heterodimers.

237 Thus, TAP1-TAP2 NBD dimers are not fully stabilized by nucleot

238 The TAP heterodimer (TAP1-TAP2) introduces the final component of the MHC cla

239 nsporter associated with antigen processing (Tap1-Tap2).

240 e MHC class I genes in the complex, arranged TAP1-TAP2-UAA-UBA-UCA-UDA-UEA The UAA gene, situated pro

241 exchanged chimeras as well as to a truncated TAP1.TAP2 complex containing just the membrane-spanning

242 otides stabilize the peptide binding site of TAP1.TAP2 complexes against inactivation, and enhanced t

243 The enhanced structural stability of TAP1.TAP2 complexes in the presence of tapasin might exp

244 for peptide transport, and ATP hydrolysis by TAP1.TAP2 complexes is important for transport activity.

245 equired for high affinity peptide binding to TAP1.TAP2 complexes, and in fact, the presence of tapasi

246 Two nucleotide binding sites are present in TAP1.TAP2 complexes.

247 Peptide translocation is undetectable for TAP1.TAP2(K509M) complexes, but low levels of translocat

248 ced nucleotide binding at the TAP2 site upon TAP1/TAP2 complex formation.

249 The TAP1/TAP2 complex is required for peptide translocation

250 tand the role of each structural unit of the TAP1/TAP2 complex, we generated two chimeras containing

251 ssively reduced efficiencies relative to the TAP1/TAP2 complex.

252 whether the two nucleotide-binding sites of TAP1/TAP2 complexes also differed in their nucleotide bi

253 TAP1/TAP2 complexes translocate peptides from the cytoso

254 e binding to a cytosol-accessible surface of TAP1/TAP2 complexes, but the location of the TAP peptide

255 eractions occurring on the cytosolic face of TAP1/TAP2 complexes, we investigated quaternary associat

256 sites with relatively similar affinities in TAP1/TAP2 complexes.

257 n of TAP1 and TAP2, or of oligomerization of TAP1/TAP2 heterodimers on membranes.

258 No such effects were observed for the TAP1/TAP2 interaction or the complex formation between T

259 TAP1/TAP2 NBD interactions appear to contribute at least

260 opsies showed that H-RS cells were uniformly TAP1/TAP2-positive and expressed HLA class I in the majo

261 The TAP1-TAP2iso transporter facilitated the maturation of M

262 Importantly, TAP1-TAP2iso transporters expressed in T2 cells exhibite

263 and cell lines for genetic abnormalities in TAP1 that might have led to an acquired loss of antigen

264 of US6 with TAP stabilizes a conformation in TAP1 that prevents ATP binding and subsequent peptide tr

265 lanking HLA-DNA to RING3 (45 kb), as well as TAP1 to TAP2 (15 kb), by use of independent CEPH haploty

266 study, we map the respective binding site in TAP1 to the polar face of the amphipathic TM helix TM9 a

267 Only IFN gamma increases TAP1 transcription assessed by reporter gene assay.

268 immune system in RRP is by interfering with TAP1 (transporter associated with antigen presentation 1

269 Beta-2 microglobulin and TAP1 (transporter associated with antigen processing 1)

270 esidues involved in peptide binding, such as TAP1-Val-288, TAP2-Cys-213, TAP2-Met-218.

271 onema denticola was utilized to determine if Tap1 was essential for motility.

272 coupled with Western blotting revealed that Tap1 was located in the aqueous phase.

273 nt cytosolic transcription factor, LMP2, and TAP1 was observed.

274 iated antigen peptide transporter subunit 1 (Tap1), was confirmed after transfection of a neuronal ce

275 FlgE and Tap1 were further characterized.

276 duced affinity for nucleotides compared with TAP1, when the two proteins were separately expressed.