ライフサイエンスコーパス: TBSV

1 on in yeast, which supports replication of a TBSV replicon RNA (repRNA), reduced repRNA accumulation

2 increased or decreased the accumulation of a TBSV replicon RNA.

3 he remainder decreased the accumulation of a TBSV replicon RNA.

4 d RdRp could perform de novo initiation on a TBSV plus-strand RNA template in the presence of the p33

5 Application of this methodology produced a TBSV DNA-based gene vector which yielded readily detecta

6 ents with ssRNA revealed that p33 binds to a TBSV-derived sequence with higher affinity than to other

7 elective p33 binding in vitro also abolished TBSV RNA replication both in plant and in Saccharomyces

8 In addition, TBSV-NPs do not show capsomeric vacancies after surpassi

9 ial and nonessential host genes could affect TBSV recombination and evolution.

10 O2, is involved in antiviral defense against TBSV.

11 rping the GTP-Rab5-positive endosomes allows TBSV to build a PE-enriched viral replication compartmen

12 This strategy likely allows TBSV to protect the replicating viral RNA from degradati

13 Altogether, this replication strategy allows TBSV to separate minus- and plus-strand syntheses in tim

14 tibility and restriction factors for BMV and TBSV have been identified using yeast as a model host.

15 The dependence of TMV, PVX, and TBSV on intact microfilaments for intercellular movement

16 is shown to be directly associated with anti-TBSV RNA silencing, while its inactivation does not infl

17 The assembled TBSV replicase performed a complete replication cycle, s

18 was shown in a cell-free yeast extract-based TBSV replication assay, in which Pkc1p likely phosphoryl

19 s within the CRAC and CARC sequences blocked TBSV replication in yeast and plant cells.

20 n-dependent protein catabolism affected both TBSV replication and the cytotoxicity of a mutant huntin

21 ction of the endoribonucleolytically cleaved TBSV RNA in yeast.

22 ing that the 3' portion of the miRNA-cleaved TBSV RNAs served as a template for negative-strand RNA s

23 o led to decreased production of the cleaved TBSV RNA, suggesting that in plants, RNase MRP is involv

24 tive mutants of plant Rab5 greatly decreases TBSV replication and prevents the redistribution of PE t

25 fects the recruitment of host factors during TBSV replication.

26 lin have similar inhibitory functions during TBSV replication, although some of the details of their

27 As were amplified to very high levels during TBSV infection.

28 al replication proteins that is critical for TBSV replication.IMPORTANCE One intriguing aspect of vir

29 irming that CypA is a restriction factor for TBSV.

30 in Saccharomyces cerevisiae, is required for TBSV replication in the yeast model host.

31 t cytosolic chaperone, which is required for TBSV replication.

32 ol, suggesting that sterols are required for TBSV replication.

33 stranded RNA that serves as the template for TBSV replication.

34 TPR-containing yeast proteins in a cell-free TBSV replication assay and identified the Cns1p cochaper

35 well described by the A subunit pentons from TBSV.

36 wever, the source of energy required to fuel TBSV replication is unknown.

37 l-free system was also capable of generating TBSV RNA recombinants with high efficiency.

38 ions in viral shell stability and identifies TBSV-NPs as malleable platforms based on protein cages f

39 applying a chloride channel blocker impeded TBSV replication in Nicotiana benthamiana protoplasts or

40 tin-conjugating enzyme function of Cdc34p in TBSV replication.

41 nderstanding of the roles of host factors in TBSV replication, we have tested the effect of Rsp5p, wh

42 er our understanding on the role of GAPDH in TBSV replication, we used an in vitro TBSV replication a

43 To dissect the function of Hsp70 in TBSV replication, in this paper we use an Hsp70 mutant (

44 ing that in plants, RNase MRP is involved in TBSV RNA degradation.

45 e large family of host prolyl isomerases, in TBSV replication.

46 ning the relevance of these host proteins in TBSV replication.

47 e; APB) leads to a 3- to 5-fold reduction in TBSV replication in yeast.

48 cleaves the TBSV RNA in vitro, resulting in TBSV RNA degradation products similar in size to those o

49 at the co-opted GAPDH plays a direct role in TBSV replication by stimulating plus-strand synthesis by

50 otein, play partially complementary roles in TBSV replication in cells and in cell extracts.

51 that cytosolic Hsp70 plays multiple roles in TBSV replication, such as affecting the subcellular loca

52 our understanding of the role of sterols in TBSV replication, we demonstrate that the downregulation

53 e downregulation of Rsp5p leads to increased TBSV accumulation.

54 itor of Pkc-like kinases, leads to increased TBSV replication in yeast, in plant single cells, and in

55 sive temperature, TS Cns1p could not inhibit TBSV replication.

56 tive mutant of CypA was also able to inhibit TBSV replication in vitro due to binding to the replicat

57 ll, blocking Gef1p function seems to inhibit TBSV replication through altering Cu(2+) ion metabolism

58 Overexpression of Cns1p inhibited TBSV replication in yeast.

59 nant CypA, Roc1, and Roc2 strongly inhibited TBSV replication in a cell-free replication assay.

60 in many cellular processes, which inhibited TBSV replication when overexpressed.

61 terestingly, recombinant Rsp5p also inhibits TBSV RNA replication in a cell-free replication assay, l

62 e find that overexpression of Rsp5p inhibits TBSV replication in Saccharomyces cerevisiae yeast, whil

63 Moreover, APB treatment inhibits TBSV RNA accumulation in plant protoplasts and in Nicoti

64 -free system also replicated the full-length TBSV genomic RNA, which resulted in production of subgen

65 e host factors, while unlike the full-length TBSV RdRp, the truncated RdRp did not need the viral p33

66 gether, our data reveal that Gef1p modulates TBSV replication via regulating Cu(2+) metabolism in the

67 dual tomato bushy stunt virus nanoparticles (TBSV-NPs).

68 mechanical deformations performed on native TBSV-NPs induce an analogous result.

69 ospholipids, sterols, and the actin network, TBSV exerts supremacy over the host cell to support vira

70 plementary RNA as template to synthesize new TBSV replicon RNA.

71 io of TBSV recombinants to the nonrecombined TBSV RNA.

72 y associated with duplex approximately 21-nt TBSV siRNAs, while P19/75-78 does not bind these molecul

73 n complex that contained approximately 21-nt TBSV-derived siRNAs and that exhibited ribonuclease acti

74 al preparations, suggesting that assembly of TBSV and CIRV replicases could take place in the purifie

75 ion of the lethal syndrome characteristic of TBSV infections.

76 cannot prevent RISC-mediated degradation of TBSV RNA and thus reduce viral pathogenicity.

77 s for ribonuclease activity and detection of TBSV-derived siRNAs.

78 y of p92(pol), with consequent inhibition of TBSV replicase activity.

79 teractions, is responsible for inhibition of TBSV replication, whereas the HECT domain, involved in p

80 east Cpr1p cyclophilin, a known inhibitor of TBSV replication in yeast.

81 nd Hsp90 chaperones as a strong inhibitor of TBSV replication.

82 rting the idea that Pkc1p is an inhibitor of TBSV RNA replication.

83 kc-related pathways are potent inhibitors of TBSV in several hosts.

84 ive mutant of Pkc1p revealed a high level of TBSV replication at a semipermissive temperature, furthe

85 2) yeast strain that supports a low level of TBSV replication.

86 uplicated AU-rich sequences, the majority of TBSV DI RNA recombinants were imprecise.

87 ase experiments showed that the mechanism of TBSV replication involves the use of dsRNA templates in

88 t either increased or decreased the ratio of TBSV recombinants to the nonrecombined TBSV RNA.

89 wide screens reveals that the replication of TBSV and brome mosaic virus (BMV), which belongs to a di

90 nce of cytosolic Hsp70 in the replication of TBSV and other plant viruses in a plant host.

91 transferase in yeast enhances replication of TBSV and other viruses, suggesting that abundant PE in s

92 capsid is essentially identical with that of TBSV, and the T=1 particles are well described by the A

93 The inhibitory effect of APB on TBSV replication can be complemented by exogenous stigma

94 ry effect of deletion of CCC2 copper pump on TBSV replication in yeast, while altered iron metabolism

95 ranscribed in vitro were mixed with parental TBSV transcripts and inoculated into protoplasts or plan

96 , uses a similar strategy to the peroxisomal TBSV to hijack the Rab5-positive endosomes into the vira

97 ber of the Tombusviridae which permits rapid TBSV-mediated foreign-gene expression upon direct rub-in

98 Using purified recombinant TBSV and CIRV replication proteins, we showed that TBSV

99 replicase required two purified recombinant TBSV replication proteins, which were obtained from E. c

100 ilencing complex cleavage of the recombinant TBSV RNAs.

101 ion of an N-terminally truncated recombinant TBSV RdRp.

102 while altered iron metabolism did not reduce TBSV replication.

103 rthologs of ERG25, in N. benthamiana reduced TBSV RNA accumulation but had a lesser inhibitory effect

104 ol biosynthesis inhibitor lovastatin reduced TBSV replication by 4-fold, confirming the importance of

105 t observed in rpn11 mutant yeast by reducing TBSV recombination.

106 levels in yeast and plant cells replicating TBSV.

107 ained from yeast and plant cells replicating TBSV.

108 surrogate host and plant leaves replicating TBSV.

109 T-I or ESCRT-III deletion yeasts replicating TBSV RNA, demonstrating the requirement for these co-opt

110 med in ESCRT-III deletion yeasts replicating TBSV RNA.

111 ete replication cycle on added plus-stranded TBSV replicon RNA (repRNA) that led to the production of

112 ties of p33 to bind to sterol and to support TBSV replication in yeast and plant cells.

113 in Saccharomyces cerevisiae, which supports TBSV replication.

114 in this paper, the authors demonstrate that TBSV co-opts the guanosine triphosphate (GTP)-bound acti

115 Here, we demonstrate that TBSV p33 and p92 replication proteins can bind to sterol

116 Altogether, we demonstrated that TBSV is less limited while CIRV is more restricted in ut

117 We find that TBSV co-opts the cellular glycolytic ATP-generating pyru

118 nd CIRV replication proteins, we showed that TBSV could use the purified yeast ER and mitochondrial p

119 pids are the most efficient, suggesting that TBSV replicates within membrane microdomains enriched fo

120 ate for negative-strand RNA synthesis by the TBSV RNA-dependent RNA polymerase (RdRp), followed by te

121 highly purified yeast RNase MRP cleaves the TBSV RNA in vitro, resulting in TBSV RNA degradation pro

122 The formation of the TBSV replicase required two purified recombinant TBSV re

123 d transport both affected replication of the TBSV replicon and enhanced the cytotoxicity of the Parki

124 to the in vivo situation, replication of the TBSV replicon RNA took place in a membraneous fraction,

125 reduced or increased the accumulation of the TBSV replicon.

126 atitis C virus to specifically recognize the TBSV IRE.

127 In addition to faithfully replicating the TBSV replicon RNA, the cell-free system was also capable

128 The experiments demonstrated that the TBSV (-)RNA is present as a double-stranded RNA that ser

129 replication, in this work we showed that the TBSV p33 and p92 replication proteins could bind to ster

130 The data support the notion that the TBSV replication proteins are associated with sterol-ric

131 We found that this RNA sequence bound to the TBSV replicase proteins more efficiently than did contro

132 el the mechanism of PE enrichment within the TBSV replication compartment, in this paper, the authors

133 studies with tomato bushy stunt tombusvirus (TBSV) in a yeast model host have revealed the inhibitory

134 us work with Tomato bushy stunt tombusvirus (TBSV) in model host yeast has revealed essential roles f

135 Tomato bushy stunt tombusvirus (TBSV) is a model virus that can replicate a small replic

136 d to inhibit Tomato bushy stunt tombusvirus (TBSV) replication in a Saccharomyces cerevisiae model ba

137 s works with Tomato bushy stunt tombusvirus (TBSV) revealed the recruitment of either peroxisomal or

138 Wild-type TBSV or p19-defective mutants initially show a similar i

139 However, unlike TBSV, there appears to be a novel zinc binding site with

140 By using TBSV RdRp, we show that the co-opted cellular Hsp70 chap

141 plicase complex of Tomato bushy stunt virus (TBSV) and affects asymmetric viral RNA synthesis.

142 ering (DI) RNAs of tomato bushy stunt virus (TBSV) and have investigated their potential to protect t

143 Tomato bushy stunt virus (TBSV) and other tombusviruses encode a p19 protein (P19)

144 The VRCs built by Tomato bushy stunt virus (TBSV) are enriched with phosphatidylethanolamine (PE) th

145 Tomato bushy stunt virus (TBSV) cDNA, positioned between a modified cauliflower mo

146 Tomato bushy stunt virus (TBSV) co-opts cellular ESCRT (endosomal sorting complexe

147 rall, the works on Tomato bushy stunt virus (TBSV) have revealed intriguing and complex functions of

148 in degradation of Tomato bushy stunt virus (TBSV) in a Saccharomyces cerevisiae model host, we teste

149 the replication of Tomato bushy stunt virus (TBSV) in a yeast model host.

150 RNA replication of Tomato bushy stunt virus (TBSV) in yeast cell-free extracts and in plant extracts.

151 er (+)RNA viruses, tomato bushy stunt virus (TBSV) induces major changes in infected cells.

152 busvirus, of which tomato bushy stunt virus (TBSV) is the type member.

153 hat replication of Tomato bushy stunt virus (TBSV) leads to the formation of double-stranded RNA (dsR

154 in replication of Tomato bushy stunt virus (TBSV) model (+)RNA virus.

155 he closely related Tomato bushy stunt virus (TBSV) or Cucumber necrosis virus (CNV) in a yeast model

156 tiana benthamiana, Tomato bushy stunt virus (TBSV) P19 suppressor mutants are very susceptible to RNA

157 activation of the Tomato bushy stunt virus (TBSV) RdRp requires a soluble host factor(s).

158 (PE) vesicle-based Tomato bushy stunt virus (TBSV) replication assay.

159 s interacting with Tomato bushy stunt virus (TBSV) replication proteins in a genome-wide scale, we ha

160 ously we described Tomato bushy stunt virus (TBSV) vectors, which retained their capsid protein gene

161 the replication of tomato bushy stunt virus (TBSV), a positive-strand RNA virus of plants.

162 ing replication of Tomato bushy stunt virus (TBSV), a small model plant virus, we screened 800 yeast

163 A recombination in Tomato bushy stunt virus (TBSV), a small model plant virus.

164 ing replication of Tomato bushy stunt virus (TBSV), a small model positive-stranded RNA virus, we ove

165 the replication of Tomato bushy stunt virus (TBSV), a small tombusvirus of plants, we have developed

166 ion of the RdRp of Tomato bushy stunt virus (TBSV), a small tombusvirus of plants, we used N-terminal

167 Tomato bushy stunt virus (TBSV), a tombusvirus with a nonsegmented, plus-stranded

168 te associated with Tomato bushy stunt virus (TBSV), a tombusvirus, undergoes frequent recombination i

169 the p19 protein of tomato bushy stunt virus (TBSV), that prevents the onset of PTGS in the infiltrate

170 pical tombusvirus, Tomato bushy stunt virus (TBSV), we show that recombinant p33 replicase protein bi

171 cation proteins of Tomato bushy stunt virus (TBSV), which is a small, plus-stranded RNA virus.

172 similar to that of Tomato bushy stunt virus (TBSV), with major differences lying on the exposed loops

173 The Tomato bushy stunt virus (TBSV)-encoded p19 protein (P19) is widely used as a robu

174 plicase complex of Tomato bushy stunt virus (TBSV).

175 ic virus (BMV) and tomato bushy stunt virus (TBSV).

176 ato virus X (PVX), tomato bushy stunt virus (TBSV)], is inhibited by disruption of microfilaments.

177 PDH in TBSV replication, we used an in vitro TBSV replication assay based on recombinant p33 and p92(

178 hat exhibited ribonuclease activity that was TBSV sequence-preferential, ssRNA-specific, divalent cat

179 By two weeks postinoculation with TBSV, all untransformed N. benthamiana plants and transf

180 Also, as was seen with TBSV, CNV appears to have a calcium binding site between