ライフサイエンスコーパス: TCR-beta

1 TCR beta-chain CDR3-length distribution analysis using P

2 TCR beta-chain contacts are mostly through the variable

3 TCR beta-chain repertoires were polyclonal in infants.

4 TCR beta-chain V region gene diversity was determined by

5 TCR-beta and CDR-beta gene usage of single islet-infiltr

6 TCR-beta gene recombination and allelic exclusion were n

7 a similar increase was not observed in GT(0)/TCR-beta(0) mice, and the titer of alphaGal-specific Abs

8 evel of alphaGal-specific serum Abs in GT(0)/TCR-beta(0) mice.

9 (0)) to generate double-knockout mice (GT(0)/TCR-beta(0)).

10 While GT(0)/TCR-beta+ mice exhibited an age-dependent increase in th

11 Treatment of GT(0)/TCR-beta+ mice with anti-CD40L Abs before immunization w

12 erum titer of alphaGal-specific Abs in GT(0)/TCR-beta+ mice, but had no effect on the level of alphaG

13 ignificantly lower than in age-matched GT(0)/TCR-beta+ mice.

14 cell pool drastically contracted to 200,000 TCR beta-chains.

15 tely flanking intron sequences of a Vbeta8.1 TCR-beta gene.

16 -J alpha 281 TCR, flow cytometry of NK1.1(+) TCR beta(+) cells, and analysis of cytokine production b

17 esults in a significant reduction of NK1.1(+)TCR-beta(+) and CD1d tetramer-positive cells, consistent

18 chronic collagen-induced arthritis in DBA/1-TCR-beta transgenic mice, as well as collagen-induced ar

19 polyclonal V beta 8, V beta 7, and V beta 2 TCR beta chains.

20 mentarity determining region 3 lengths of 24 TCR-beta chain V region families from CD4+ and CD8+ peri

21 Finally, and of importance, IL-2Ralpha(-/-) TCR-beta(-/-) mice had abrogation of liver and colon pat

22 assessed by the DNA sequence analysis of 50 TCR beta clones obtained by rapid amplification of cDNA

23 mensional structure of the complex between a TCR beta chain (mouse V beta8.2) and the SAG staphylococ

24 (TCR alpha) chains in mice transgenic for a TCR beta chain has allowed us to demonstrate a central r

25 ng a murine fasL cDNA under the control of a TCR beta-chain enhancer minigene.

26 e diseases through transient expression of a TCR beta-chain.

27 cule on an antigen- presenting cell and to a TCR beta-chain, thereby causing activation of the T cell

28 t, complementation of Rag-2(-/)- mice with a TCR beta transgene restored ICN1-induced DP development

29 is of T cell receptor (TCR) repertoires in a TCR-beta transgenic model.

30 lopment is rescued through transduction of a TCR-beta transgene.

31 s TCR-alpha chain even when it paired with a TCR-beta chain of irrelevant specificity.

32 le T-lineage cell that had stable bi-allelic TCR beta rearrangements.

33 pecific gene enhancers, including TCR-alpha, TCR-beta, and CD4.

34 mplexes comprise a disulfide-linked pT alpha-TCR-beta heterodimer associated not only with CD3-gamma

35 e Vbeta8.1/8.2 TCR that is predominant among TCR-beta+ T cells.

36 In this study, we analyze TCR-beta recombination and cell cycle status with respec

37 rangement and allelic exclusion, we analyzed TCR beta chromatin structure in double negative (DN) thy

38 We analyzed TCR beta-chain and alpha-chain genes expressed by these

39 rs encoding the HIV-1-specific TCR alpha and TCR beta chains cloned from a CTL clone specific for an

40 3 proteins are expressed before pT alpha and TCR beta-chains in prothymocytes and are expressed intra

41 lex may function independent of pT alpha and TCR beta.

42 to accommodate juxtaposition of CD3gamma and TCR beta ectodomains and foster quaternary change that c

43 situated underneath the TCR alpha-chain and TCR beta-chain, respectively.

44 Both TCR alpha-chains and TCR beta-chains made contact with the CD1d molecule with

45 HSV-specific lesional CD4(+) cell clones and TCR beta -variable (TCRBV) sequencing confirmed that the

46 ns displayed differential MR1 dependency and TCR beta-chain bias, consistent with possible divergent

47 uction, homeostatic T cell proliferation and TCR beta-chain diversity in young (approximately 25 year

48 unique phenotypic traits of CD8(+) TILs and TCR beta chain (TCRbeta) clonotypic frequency in melanom

49 enic mice devoid of endogenous TCR-alpha and TCR-beta chains.

50 e the initiation of clonotypic TCR-alpha and TCR-beta gene rearrangement but is down-regulated in mos

51 method for expressing defined TCR-alpha and TCR-beta proteins from a single 2A peptide-linked multic

52 hus, we examined nucleotide loss from Ig and TCR-beta coding joints in mice lacking p53.

53 affinity purified on anti-TCR-alpha and anti-TCR-beta mAb columns had identical CS-protective activit

54 +) T cells transcribe Il2 within 6 h of anti-TCR-beta plus anti-CD28 stimulation (early phase).

55 These cells function redundantly, as TCR-beta(-/-) and TCR-delta(-/-) mice were both resistan

56 B6 TCR beta-chain knockout (KO) recipients of a myelin olig

57 nscripts increased the levels of PTC-bearing TCR-beta transcripts in the nuclear fraction of cells.

58 productive "in-frame" T cell receptor beta (TCR beta), immunoglobulin (Ig) heavy (H) or Ig light (L)

59 n in-frame T cell receptor for antigen beta (TCR-beta) gene rearrangement will continue to mature.

60 The random nature of T-cell receptor-beta (TCR-beta) recombination needed to generate immunological

61 53(-/-) mice die of T-cell receptor-beta(-) (TCR-beta(-)) thymic lymphomas with translocations and ot

62 l receptor (TCR) repertoire in terms of both TCR-beta usage and clonal composition.

63 es and criteria for lineage choice when both TCR-beta and gammadelta-TCR are simultaneously expressed

64 human and mouse MAIT cells expressed a broad TCR-beta repertoire, and although the majority of human

65 s recognizing TCR Vbeta chain, as well as by TCR beta gene rearrangements.

66 ression and facilitates signaling from a CD3-TCR beta complex.

67 expressing IFN-gamma and of NKp46/CD335(+), TCR-beta(+) cells expressing IL-13.

68 h) cells stained positive for CD2, CD3, CD8, TCR beta-chain, and NK1.1 but did not express the B cell

69 with surface determinants for CD3, CD4, CD8, TCR-beta, or TCR-delta molecules.

70 erantigens stimulate T cells bearing certain TCR beta-chain variable regions when bound to MHC II mol

71 -cell-deficient (T-cell receptor beta chain [TCR-beta] -/- or TCR-betaxdelta -/-) mice was shown to e

72 Using clonotypic TCR beta-chain length and sequence analysis we confirmed

73 n a buried hydroxymethyl that forms a common TCR beta-chain V region variant.

74 by directly targeting the murinized constant TCR beta domain (TCRmu) with a zirconium-89 ((89)Zr)-lab

75 e considered to be associated with defective TCR beta-chain rearrangement.

76 mma delta cells, newborn TCR beta-deficient (TCR beta(-/-)) thymi were grafted to IL-7(-/-) mice.

77 age clearance in T-cell receptor beta/delta (TCR-beta/delta) knockout (KO) and IgH-mu KO mice, respec

78 ntained at approximately 2 x 10(7) different TCR beta-chains.

79 hannel catfish revealed distinctly different TCR beta rearrangements.

80 ower bound for the total number of different TCR beta (TCRB) sequences in human repertoires.

81 However, these cells have diverse TCR beta-chains, including Vbeta8, Vbeta7, and Vbeta2 in

82 d the residues in the CDR3 region of the DN1 TCR beta-chain that were predicted to project between th

83 cell clones expressing the in vivo dominant TCR beta-chain sequences were identified in three patien

84 the rearrangement potential of Dbeta2 during TCR beta locus assembly.

85 sequences of 55 spleen cDNA clones encoding TCR-beta genes are compared, and 7 diverse Vbeta familie

86 xpress both transgene derived and endogenous TCR beta chains.

87 ) that promoted the disruption of endogenous TCR beta- and alpha-chain genes.

88 drives gene rearrangement at the endogenous TCR beta locus and results in the appearance of Vbeta5(-

89 ific transgenic mice lacking only endogenous TCR-beta chains also developed EAE, suggesting that in T

90 s, we designed a system where the endogenous TCR-beta is knocked out from the recipient cells using c

91 , cells with high protective activity escape TCR-beta chain allelic exclusion.

92 They exhibit TCR-beta gene loci in germline configuration and show lo

93 hese IEL from lck-/-fyn-/- animals exhibited TCR beta-gene rearrangement.

94 On introduction of exogenous TCR-beta chains, but not of TCR-alpha chains, assembly a

95 V CTL clones were all Vbeta13+ and expressed TCR beta-chains with highly homologous complementarity-d

96 th impaired Th2 cytokines, IL-17A expressing TCR beta (+) T cells were increased, while IL-22 express

97 HC and how they stimulate T cells expressing TCR beta chains from a number of different families, res

98 negative selection of thymocytes expressing TCR beta-chains reactive against several retroviral supe

99 ed by one or two clones typically expressing TCR beta-chain variable TRBV-15.

100 ve (DN) thymocytes, which are permissive for TCR beta recombination, and in double positive (DP) thym

101 This finding indicates a role for TCR beta in defining natural killer T cell specificity,

102 that Rho acts as an intracellular switch for TCR beta selection, the critical thymic-differentiation

103 tes exhibited a lower rate of mortality from TCR-beta(-) tumors, which harbored significantly elevate

104 Assembly of a functional TCR beta-chain gene triggers feedback inhibition of V(be

105 l cells just before selection for functional TCR beta-chain expression.

106 l features of this interaction, we generated TCR beta-chain transgenic mice using a TCR derived from

107 s can be explained predominantly by germline TCR-beta locus factors and not TCR-beta allelic or HLA e

108 e Valpha24 Vbeta11 clones were shown to have TCR-beta CDR3 diversity and express the natural killer (

109 es an entirely different, more heterogeneous TCR beta-chain repertoire that fails to recognize specif

110 n expanded rapidly after OPC, exhibited high TCR-beta clonal diversity, and was absent in Rag1(-/-),

111 Overall, homologous TCR beta- and alpha-chains showed identical V regions an

112 s, defined as those that expressed identical TCR beta-chain amino acid sequences and recurred in mult

113 In TCR beta locus, rearrangement initiates at two D-J casse

114 In TCR beta mice tetramer-positive thymocytes are detectabl

115 esponding to NP were dramatically altered in TCR beta(-/-) mice.

116 nfirmed severe inflammation in Wt but not in TCR-beta x delta(-/-) mice.

117 urs in gammadelta-selected DN3 thymocytes in TCR-beta-/- mice and in IL2-GFP transgenic reporter mice

118 ties that are likely conferred by individual TCR beta-chain rearrangements.

119 Interestingly, cross-analysis of individual TCR beta repertoires revealed a set >10,000 of the most

120 toire, with a median frequency of individual TCR beta-chain sequences of 1 in 2.4 x 10(7) CD4 T cells

121 habeta-lineage differentiation by inhibiting TCR-beta formation.

122 reduced amounts of surface and intracellular TCR-beta protein and decreased levels of tcrbeta transcr

123 ta selection, concomitant with intracellular TCR-beta expression.

124 Individual residues within the MAIT TCR beta chain were dispensable for the interaction with

125 In RA patients, the median TCR beta-chain frequency was increased 10-fold, indicati

126 ase manifestations in T cell-deficient mice (TCR-beta x delta(-/-)), although it resulted in bacterem

127 ce were crossed with TCR-beta knockout mice (TCR-beta(0)) to generate double-knockout mice (GT(0)/TCR

128 While pT alpha(a) acts to retain most TCR beta-chains intracellularly, pT alpha(b) permits hig

129 eature was also observed for human and mouse TCR beta chains, although the alpha and beta chain V-gen

130 By using specific mAbs to mouse TCR-beta (H57) and CD3epsilon (2C11) subunits, we herein

131 binding site with specificities for multiple TCR beta-chains.

132 e co-ordinates of a recently reported murine TCR beta-chain crystal structure, and other documented i

133 hat contribute to the activity of the murine TCR beta enhancer in mature and immature T cell lines.

134 m of the D beta 1 gene segment in the murine TCR beta locus was deleted to assess its role in control

135 evelopment of TCR gamma delta cells, newborn TCR beta-deficient (TCR beta(-/-)) thymi were grafted to

136 These findings indicate that NKT TCR beta-chain diversity results in differential and non

137 No TCR beta chain restriction was found.

138 n thymocytes, resulting in a burst of normal TCR beta and delta rearrangements.

139 y by germline TCR-beta locus factors and not TCR-beta allelic or HLA effects.

140 revision requires the rearrangement of novel TCR beta-chain genes and depends on recombinase-activati

141 Structural analysis of TCR beta-chain usage in such patients demonstrated a jun

142 R repertoires focus solely on an analysis of TCR beta-chains, rather than the combined TCRalphabeta h

143 J12 in humans), which pairs with an array of TCR beta-chains.

144 To investigate chromatin control of TCR beta rearrangement and allelic exclusion, we analyze

145 ue was used to determine the distribution of TCR beta-chain V region sequences expressed in the trans

146 ween beta selection and allelic exclusion of TCR beta.

147 promoter that drives germline expression of TCR beta gene segments in vivo.

148 survival of thymocytes lacking expression of TCR beta, showing hallmarks of hyperactive Notch signali

149 hymocytes are selected for the expression of TCR beta-chains with shorter CDR3 at the double-positive

150 lity to proliferate, which is independent of TCR beta-chain CDR3 sequence or precursor frequency.

151 a multiplex PCR assay for the CDR3 length of TCR beta-chains, we have found a striking increase in th

152 subpopulations, known to be the location of TCR beta-chain rearrangement.

153 entarity-determining region 3 (CDR3) loop of TCR beta chains.

154 synovial fluid was analyzed using a panel of TCR beta variable region-specific monoclonal antibodies.

155 e deep and precisely normalized profiling of TCR beta repertoires in 39 healthy donors aged 6-90 y.

156 cell clone had functional rearrangements of TCR beta-chain genes using the Vbeta120.la and Jbeta1.1

157 Reconstitution of TCR beta-chain KO mice with wild-type spleen cells halte

158 The variable region of TCR beta-chain (Vbeta) gene usage was determined by fluo

159 diate Vav-1 action as critical regulators of TCR beta selection.

160 otpads with reovirus, and the repertoires of TCR beta-chains expressed on virus-specific CD8(+) T cel

161 Then, we applied CDR3 spectrotyping of TCR beta-chain to assess the clonality of T cells at dif

162 vitro studies to be located at the stages of TCR beta-chain rearrangement.

163 Furthermore, deep-sequencing analysis of TCR-beta (TRB) and TCR-alpha (TRA) rearrangements of CD3

164 stant alpha domains, paired with an array of TCR-beta chains.

165 beta region, are involved in the control of TCR-beta allelic exclusion.

166 tion of immune cells into the spinal cord of TCR-beta x delta(-/-) mice was reduced and the resident

167 mice with p53 deletion in thymocytes die of TCR-beta(-) tumors containing Tcralpha/delta translocati

168 ositive thymocytes, diminished expression of TCR-beta, and increased expression of CD25, suggesting a

169 We further show that the down-modulation of TCR-beta expression requires contact between S.

170 ibition of NMD induces premature shut-off of TCR-beta rearrangement.

171 D4(-)CD8(-) thymocytes during the process of TCR-beta chain rearrangement by a recombinase-independen

172 third complementarity-determining region) of TCR-beta.

173 We found that the down-regulation of TCR-beta transcripts in response to nonsense codons requ

174 monkeys exhibited the dominant responses of TCR-beta complementarity-determining region 3-restricted

175 sive immune profiling and deep sequencing of TCR-beta V regions, two subsets of cTregs, based on expr

176 dily detectable in normal T1 cells, but only TCR-beta intermediates were detected in IL-7R-/- T1 cell

177 f secondary infection, we observed that only TCR-beta deficiency or simultaneous neutralization of IL

178 ymocytes die at later ages to TCR-beta(-) or TCR-beta(+) thymic lymphomas containing a similar patter

179 In contrast, CD4-/- or TCR-beta-/- mice develop polymicrobial sepsis and end-or

180 sets of CD8(+) cells expressing a particular TCR beta-chain variable region were more commonly identi

181 specificity of different SAGs for particular TCR beta chains, and for the observed influence of the T

182 on proliferation of T cells with particular TCR beta-chains, which occurs upon recognition of virall

183 o imply that the polymorphism of the porcine TCR beta segments is similar to that found in human.

184 Pak2 was required for pre-TCR beta-selection and positive selection.

185 lon treatment that mimics the process of pre-TCR beta-selection of thymocytes to the double positive

186 found that expression of these preassembled TCR beta-chains did not downregulate recombinational acc

187 Public TCR beta-chain sequences were identified across differen

188 biased and frequently dominated by a public TCR beta-chain encoded by the variable gene segment TRBV

189 et >10,000 of the most representative public TCR beta clonotypes, whose abundance among the top 100,0

190 an and murine T cells have shown that public TCR beta-chain rearrangements can dominate the Ag-specif

191 g three approaches: (a) in vivo quantitative TCR beta chain V segment and complementarity determining

192 tion of a pre-TCR that includes a rearranged TCR beta-chain and the pre-TCR alpha-chain.

193 -TCR alpha-chain (pT alpha) and a rearranged TCR beta-chain assemble to form the pre-TCR that control

194 selection, mice transgenic for a rearranged TCR beta-chain derived from conventional alphabeta T lym

195 expressing diverse, endogenously rearranged TCR beta chains.

196 requires a signal from the newly rearranged TCR beta chain.

197 Dbeta1-like sequence in numerous rearranged TCR beta cDNA suggests the existence of two D-J clusters

198 Selection of a productively rearranged TCR beta-chain is the first stage in the process and occ

199 In mice expressing single, rearranged TCR beta-chains, individual mutation of amino acids in t

200 Thus, in vivo expression of the rearranged TCR beta-chain from a thymus-derived NK1.1+ Valpha14+ T

201 ed by the introduction of a fully rearranged TCR-beta transgene that precludes generation of out-of-f

202 in the absence of a functionally rearranged TCR-beta allele.

203 CTL receptor TCR beta-chain variable gene subfamilies were polyclonal

204 toxin B (SEB) mutants to soluble recombinant TCR beta chain and to the human MHC class II molecule HL

205 contact occurs, a factor capable of reducing TCR-beta expression is secreted.

206 he magnitude of the CD8(+) T cell responses, TCR beta-chain repertoires did not significantly differ

207 eukemic cells from the twins shared the same TCR beta gene rearrangement with an identical 11 bp N re

208 at >90% of the hybridomas expressed the same TCR beta-chain variable region (V(beta)10), and sequenci

209 sponding cells were determined by sequencing TCR beta chain variable (TCRBV) genes.

210 Additionally, the sequences of several TCR beta-chain CDR3 regions were homologous to TCR beta-

211 We found that CTL clones sharing TCR beta-chains exhibited disparate recognition patterns

212 selection of thymocytes that express shorter TCR beta-chain complementarity-determining region 3 (CDR

213 that most alphabeta T cells express a single TCR beta chain and most B cells express single IgH and I

214 ult from multiple interactions with a single TCR beta-chain or perhaps by cross-linking two TCR.

215 f the T cell repertoire to usage of a single TCR beta-chain, Vbeta11, implying selection by Ag.

216 pha(b) permits higher levels of cell surface TCR beta expression and facilitates signaling from a CD3

217 In CD4 thymocytes expressing a fixed Tg TCR beta-chain, the associated TCRalpha sequences in wil

218 T cells lacking surface expression of the Tg TCR beta chain and expressing diverse, endogenously rear

219 We demonstrate that TCR beta diversity per 10(6) T cells decreases roughly l

220 In this study, we demonstrate that TCR beta-chain composition can dramatically influence li

221 ne is likely to be Tcrb-V13, indicating that TCR beta-chain usage is a determinant of susceptibility

222 We also found that TCR-beta (TRB) V usage was highly restricted among 70-kD

223 Our data suggest that TCR-beta selection is not affected in p53-deficient, V(D

224 The TCR beta-chain gene enhancer activates transcription and

225 The TCR beta-chain repertoire of memory T cells specific for

226 xtremely poor shape complementarity, and the TCR beta chain complementarity-determining region 3 (CDR

227 omplexes with MHC class II molecules and the TCR beta chain, provide a framework for understanding th

228 RAG-/- mice, DJ and VDJ recombination at the TCR beta locus was functional, and normal numbers of NK

229 atures important for interaction between the TCR beta-chain and the peptide/MHC complex, we immunized

230 r emphasis on recognition of the pMHC by the TCR beta-chain, evinced by an increase in shape compleme

231 ehensive analysis of genes that comprise the TCR beta variable gene (TCRBV) repertoire of the common

232 To this end, we determined the TCR beta chain (Vbeta) usage of CD8(+) T cells from thre

233 In this study, we examined the TCR beta-chain (TRB) diversity of the CD8(+) T cell resp

234 pre-existing T cells commonly expressing the TCR beta chain variable region (TCR BV) 20 and increased

235 anscription in most cell types; however, the TCR beta enhancer (E beta) stimulates PD beta function s

236 o define specific structural features in the TCR beta chain that are important for the recognition of

237 Activation of the TCR beta (Tcrb) locus in committed thymocytes is a criti

238 arison, mice with targeted disruption of the TCR beta chain and expressing no alpha beta TCR(+) cells

239 The portion of the TCR beta chain involved in antigen recognition in the sy

240 Diversity of the TCR beta chain is generated in part by a random yet intr

241 beta E6 motifs as the essential core of the TCR beta enhancer in pro-T cells.

242 hymic organs suppressed rearrangement of the TCR beta locus (but did not inhibit TCR gamma locus rear

243 Rearrangement of the TCR beta locus is normally required for development to t

244 on of a unique heterodimer consisting of the TCR beta-chain (TCRbeta) and a 33-kDa protein, FCp33.

245 and with particular variable regions of the TCR beta-chain (Vbeta).

246 expressing the variable gene segment of the TCR beta-chain 6 (Vbeta6) expanded in the spleens of mic

247 ally, we show that residue 29 in CDR1 of the TCR beta-chain affects recognition of the glutamic acid

248 recognition, in part similar to that of the TCR beta-chain and in part similar to the conventional a

249 ing size spectratyping and sequencing of the TCR beta-chain CDR3 region.

250 -ray structures, NMR characterization of the TCR beta-chain dynamics reveals significant chemical shi

251 on, the results showed that 20 to 30% of the TCR beta-chain gene (TCRB) sequences found in one joint

252 We show that the influence of the TCR beta-chain is due to a combination of Vbeta-, Jbeta-

253 ction in usage of the variable region of the TCR beta-chain over time.

254 Sequencing of the TCR beta-chain repertoire reveals that the DEJ CD8alphaa

255 MF; KF11) and identified common usage of the TCR beta-chain TRBV7 in eight of nine HLA B57 subjects e

256 and tumor regression through analysis of the TCR beta-chain V region gene products expressed in sampl

257 o far indicate increased interactions of the TCR beta-chain with the pMHC compared with their syngene

258 Typhimurium down-modulates expression of the TCR beta-chain, a molecule that is essential for Ag reco

259 rd complementarity-determining region of the TCR beta-chain.

260 ions) and an in vitro selection study on the TCR beta chain (four mutations).

261 To focus our analysis solely on the TCR beta-chain, we created a transgenic mouse expressing

262 which is consistent with the notion that the TCR beta-chain interacts in vivo preferentially with thi

263 of a particular mutant of SEC3 or SEB to the TCR beta chain, the greater its ability to stimulate T c

264 To determine whether the TCR beta-chain junctional regions influence recognition

265 OVA-8:Kb complex C terminus overlap with the TCR beta-chain footprint, but that this footprint also e

266 ifferential regulation of regions within the TCR beta locus during thymocyte development.

267 , whereas recombination intermediates at the TCR-beta locus could be detected.

268 evelopment, and clonal rearrangements in the TCR-beta genes were present in most tumors.

269 heavy chain (IgH) locus, and delayed in the TCR-beta locus.

270 ZW) mice that have a natural deletion in the TCR-beta locus.

271 f the most prominently induced site near the TCR-beta enhancer (E beta) in allelic exclusion by targe

272 Proper assembly of the TCR-beta chain into the pre-TCR complex delivers signals

273 hich revealed an unexpected influence of the TCR-beta chain on the avidity of CD1d:alpha-GalCer bindi

274 erences conferred by the Vbeta domain of the TCR-beta chain, with Vbeta8.2 conferring higher avidity

275 intron sequences from another member of the TCR-beta family also triggered strong down-regulation, s

276 In-frame rearrangement of the TCR-beta locus and expression of the pre-TCR are compuls

277 hylation, and transcription in 100 kb of the TCR-beta locus.

278 on is determined by factors intrinsic to the TCR-beta locus.

279 mechanism for this downregulation using the TCR-beta gene, which acquires PTCs as a result of progra

280 n bacterial pneumonia, mice deleted of their TCR beta- and/or delta-chain were intratracheally inocul

281 N (or minor variations) in the CDR3 of their TCR beta-chains.

282 subcapsular zone where they recombine their TCR beta-chain and gamma-chain gene loci.

283 terrelated than cTreg subsets based on their TCR-beta repertoires, but exhibited varied immune profil

284 lineage, but we also demonstrated that this TCR beta-chain was able to provide stronger TCR signals.

285 This TCR-beta-driven mechanism would thus unify former per se

286 sion of this gene, which contains only three TCR-beta exons, exhibited efficient downregulation in re

287 combination and that in CD4+ CD8+ thymocytes TCR beta allelic exclusion does not result from inaccess

288 owever, when IL-7(-/-) thymi were grafted to TCR beta(-/-) mice, no development of graft-derived TCR

289 R beta-chain CDR3 regions were homologous to TCR beta-chains identified previously in allograft arter

290 x and p53 in thymocytes die at later ages to TCR-beta(-) or TCR-beta(+) thymic lymphomas containing a

291 ntroduced TCR alpha chain and the transgenic TCR beta chain from the original cytochrome c-specific T

292 We created mice expressing a transgenic TCR-beta chain that confers high affinity for self-lipid

293 e to monoclonal tumors with a single, unique TCR-beta chain and diverse TCR-alpha chains, pinpointing

294 We used TCR beta-chain nucleotide sequencing to gain insight int

295 Experiments using TCR beta-chain knockout mice as BM donors indicated that

296 that express Valpha11 paired with non-Vbeta3 TCR beta-chains (Vbeta6, Vbeta8.1/8.2, Vbeta8.3, and Vbe

297 eptor (TCR) alpha chain paired with a Vbeta8 TCR beta chain in mice, or the homologous Valpha24-Jalph

298 inked glycosphingolipid specificity, whereas TCR beta-chains can confer heterogeneous additional reac

299 riant Valpha14-Jalpha281 TCR associated with TCR beta-chains of limited diversity.

300 GT(0) mice were crossed with TCR-beta knockout mice (TCR-beta(0)) to generate double-