ライフサイエンスコーパス: TCR-gamma

1 TCR gamma constant region gene expression levels and cli

2 TCR gamma delta cells, as well as alpha beta TCR-NKT cel

3 TCR gamma delta T cells are considered important in the

4 TCR gamma repertoire diversification was initiated on em

5 The infusion of G8 Tg (H-2Td) TCR gamma/delta cells into lethally irradiated [900 cGy

6 All cases with assessable DNA had a TCR gamma gene rearrangement, and lacked Epstein-Barr vi

7 n RI gamma-chain in TCR-gamma delta cells, a TCR-gamma delta transgenic mouse (G8) has been crossed w

8 and RAG2, each have the ability to activate TCR gamma and delta rearrangement in human kidney cells.

9 Assembly of TCR alpha and TCR gamma chain variable region genes exhibit allelic in

10 eptor alpha/beta chain (TCR alpha beta), and TCR gamma delta.

11 M CH4, T-cell receptor (TCR) alpha/beta, and TCR gamma/delta constant domain pairs, and we found that

12 of TCR-beta chain genes in nine patients and TCR-gamma chain genes in two patients.

13 munity, TCR-alpha-/- mice treated with anti- TCR-gamma/delta monoclonal antibodies or TCR-gamma/delta

14 the rejection process was inhibited by anti-TCR gamma/delta MoAbs.

15 GVHD was prevented by the injection of anti-TCR gamma/delta monoclonal antibodies.

16 were absent in recipients treated with anti-TCR gamma/delta monoclonal antibodies (MoAbs) but not an

17 ns to generate a diverse repertoire of avian TCR gamma genes early in ontogeny.

18 on C gamma gene were identified in the avian TCR gamma locus.

19 ear regression model integrating whole blood TCR gamma constant region gene expression levels and age

20 ductive rearrangement and expression of both TCR gamma and delta genes.

21 However, here we show that both TCR-gamma and -beta recombination intermediates are read

22 pha and CD8alpha/beta T cells disappear, but TCR-gamma/delta cells are unaffected by the absence of b

23 These results suggest that CD8(+) TCR gamma delta IELs do not require class I MHC for deve

24 Unlike TCR-alpha beta cells, TCR-gamma delta cells express a distinct member of the z

25 present studies sought to define the chicken TCR gamma locus.

26 nduce the appearance of transcripts for cis, TCR-gamma, or c-fos, suggesting a role for Stat5 in thei

27 Four-color TCR-gamma PCR analysis can uncover multiple distinct clo

28 ombinatorial diversity of relatively complex TCR gamma and delta loci may contribute to the remarkabl

29 mice lack TCR-alpha/beta+ cells but contain TCR-gamma/delta+ cells and a small population of a uniqu

30 firmed by expressing a hybrid TCR containing TCR-gamma chain germ-line complementarity determining re

31 In contrast, TCR gamma delta IEL development occurred efficiently in

32 In contrast, TCR-gamma delta + DETC were normal in number and appeara

33 delta cells in these mice, since a high copy TCR-gamma transgene exhibited sufficient residual expres

34 as the absolute number of thymic dendritic, TCR-gamma delta and NK1.1 T cells were equivalent to con

35 a(-/-) mice, no development of graft-derived TCR gamma delta cells occurred, indicating that extrathy

36 Donor- and host-derived TCR gamma delta cells were recovered from thymus grafts,

37 This assay detected TCR-gamma clonal GRs in 89 samples (87%) from 36 patient

38 ematopoietic progenitor cells; and (2) donor TCR gamma/delta+ cells can facilitate the alloengraftmen

39 d intrathymically, distinguishable by either TCR-gamma delta or -alpha beta surface expression.

40 mental potential is not a result of enhanced TCR-gamma gene rearrangement/expression in IL-7R(+) pro-

41 D4+ TCR-alpha-/beta+ population and expanded TCR-gamma/delta+ population present in TCR-alpha-/- mice

42 as sufficient for development of extrathymic TCR gamma delta IEL.

43 For TCR-gamma/delta (Vgamma2) transgenic mice, nearly all ga

44 However, a direct role for TCR-gamma/delta cells in protective immunity for pathoge

45 We conclude that nonradioactive PCR-SSCP for TCR-gamma gene rearrangement analysis is a useful adjunc

46 mma1/Jgamma2 consensus primers were used for TCR-gamma gene rearrangement amplification and PCR produ

47 The observed protection resulted from TCR-gamma/delta cell-mediated arrest of both viral repli

48 The addition of donor G8 TCR gamma/delta+ cells to TCD donor BM was shown to sign

49 tive method to detect T-cell receptor gamma (TCR-gamma) gene rearrangements by polymerase chain react

50 These results show that (1) host TCR gamma/delta+ cells can reject repopulating donor cel

51 In TCR gamma delta cells, IL-2-producing cells are a subset

52 netics of endogenous IL-2 RNA degradation in TCR gamma delta cells.

53 s, supporting a mechanistic role for IL-7 in TCR-gamma locus rearrangement.

54 the role of the Fc epsilon RI gamma-chain in TCR-gamma delta cells, a TCR-gamma delta transgenic mous

55 (TCR)-gamma gene rearrangement, a defect in TCR-gamma gene transcription leading to death of gamma/d

56 intraepithelial lymphocytes (IEL), including TCR gamma delta cells, can develop extrathymically.

57 In both models of HSV-1 infection, TCR-gamma/delta cells limited severe HSV-1-induced epith

58 t of the TCR beta locus (but did not inhibit TCR gamma locus rearrangement).

59 IL-7Ralpha(-/-) mice is due to insufficient TCR-gamma gene expression.

60 uced TCR expression, because intraepithelial TCR-gamma delta cells from the zeta-deficient mice did n

61 In contrast, intraepithelial TCR-gamma delta cells of G8.zeta-/- mice expressed high

62 c-myc, cis, the T-cell receptor gamma locus (TCR-gamma), and c-pim-1.

63 DNA clones with characteristics of mammalian TCR gamma chains, including canonical residues considere

64 B and TCR alpha beta cells, but lack mature TCR gamma delta cells.

65 Analysis of TCR gamma delta gene usage in the CNS showed that the on

66 TCR alpha beta cells and the development of TCR gamma delta cells are partially independent of the T

67 te the role of thymic IL-7 in development of TCR gamma delta cells, newborn TCR beta-deficient (TCR b

68 demonstrated that extrathymic development of TCR gamma delta IEL required extrathymic IL-7 production

69 To elucidate the potential function of TCR gamma/delta cells in GVHD, we have used transgenic (

70 In contrast, injection of TCR gamma/delta+ cells into irradiated (900 cGy TBI) B6.

71 These structural requirements of TCR gamma delta recognition of prenyl pyrophosphates dis

72 -host disease (GVHD) generation, the role of TCR gamma/delta expressing cells in this process has rem

73 hibitor trichostatin A released the block of TCR-gamma gene rearrangement.

74 the results show that the poor expression of TCR-gamma genes in IL-7Ralpha(-/-) mice is responsible f

75 ast partially dependent upon the presence of TCR-gamma/delta T cells in the host.

76 To define the role of TCR-gamma/delta cells in anti-HSV-1 immunity, TCR-alpha-

77 say to assess the size and V-family usage of TCR-gamma GRs in 102 concurrent and/or sequential morpho

78 eric form of CD8 is exclusively expressed on TCR gamma delta IELs and on subsets of NK cells and TCR

79 madelta T cells can express two TCR alpha or TCR gamma chains, respectively.

80 ti- TCR-gamma/delta monoclonal antibodies or TCR-gamma/delta x TCR-alpha/beta double-deficient mice w

81 o detectable B or T cells (TCR-alpha/beta or TCR-gamma/delta); (2) at least 10-fold lower levels of i

82 ponsiveness was shown not to require CD8+ or TCR-gamma/delta+ T cells or IFN-gamma.

83 Peripheral TCR gamma delta cells accumulate GFP RNA faster than end

84 we examined transcription of a prerearranged TCR-gamma transgene in IL-7Ralpha(-/-) mice, as well as

85 data supports the model that IL-7R promotes TCR-gamma gene rearrangement by regulating accessibility

86 Proper TCR gamma and delta V(D)J rearrangement during thymocyte

87 cted a high frequency of clonally rearranged TCR gamma and TCRB genes (17/20 and 15/20 cases, respect

88 esis, we compared the sequence of rearranged TCR gamma variable region 5 genes in gammadelta+ IEL and

89 7 on transcription of endogenous, rearranged TCR-gamma genes in alpha/beta lineage cells.

90 sary for the normal expression of rearranged TCR-gamma genes.

91 ossibly mediated by STAT5, of the rearranged TCR-gamma complex during development of gammadelta T cel

92 R gamma delta and NKT cells, as well as skin TCR gamma delta-dendritic epidermal T cells, indicate th

93 f transgene expression in thymic and splenic TCR gamma delta and NKT cells, as well as skin TCR gamma

94 ge commitment as a consequence of successful TCR-gamma and -delta gene rearrangement, we do not find

95 cating that extrathymic IL-7 did not support TCR gamma delta IEL generation from newborn thymic precu

96 w transplantation (BMT) confirmed that G8 Tg TCR gamma/delta cells infiltrated GVHD target tissues (s

97 GVHD target organs are responsible for G8 Tg TCR gamma/delta+ cell mediated lethality.

98 These Tg TCR gamma/delta+ cells respond vigorously to target cell

99 These results demonstrate that TCR gamma/delta cells have the capacity to cause acute l

100 PCR showed that TCR gamma variable region 5 was rearranged in gammadelta

101 d intestine synthesize IL-7, suggesting that TCR gamma delta cell development could occur in either s

102 Thus, this study demonstrates that TCR-gamma/delta cells may play an important regulatory r

103 The demonstration that TCR-gamma/delta cells play an important protective role

104 The TCR gamma translocon contains at least 5 V region genes,

105 cells to examine the effects of changing the TCR gamma junctional region sequences on reactivity to p

106 ost graft rejecting T cells that express the TCR gamma/delta heterodimer.

107 e, we report that the germ-line gene for the TCR gamma chain in a chondrichthyan, the sandbar shark (

108 o the latter, one such candidate cell is the TCR gamma/delta+ T cell.

109 sing the TCR alpha beta receptor and not the TCR gamma delta cells, which exclusively express CD8 alp

110 nd HEB permit localized accessibility of the TCR gamma and delta loci to the recombination machinery.

111 antigens and emphasize the importance of the TCR gamma CDR3 loop and adjacent residues.

112 dependent upon the junctional region of the TCR gamma chain and upon pairing of V gamma 2 and V delt

113 Substitution of the TCR gamma junctional region (N and J) sequences from an

114 ubsets segregated on the basis of use of the TCR gamma-chain or delta-chain indicated the existence o

115 lls can be divided into subsets based on the TCR gamma-chains they express.

116 Substitution of only the TCR gamma N nucleotide region with that from this Ag-non

117 Damage to the TCR gamma-2 chain repertoire and depletion of CD56+ Vgam

118 Northern blot analysis with the TCR gamma cDNA probe revealed 1.9-kb transcripts in the

119 Thus, IL-7 controls recombination at the TCR-gamma locus by regulating locus accessibility.

120 T cell-specific locus control region for the TCR-gamma locus.

121 Production of sterile transcripts from the TCR-gamma locus, a process that generally precedes rearr

122 Instead, the TCR-gamma locus was shown to be methylated in IL-7Ralpha

123 ding from IL-7Ralpha to rearrangement of the TCR-gamma locus requires the gammac receptor chain and t

124 ignal, no initiation of recombination of the TCR-gamma locus was observed, whereas recombination inte

125 , we analyze the recombination defect of the TCR-gamma locus.

126 Most of the TCR-gamma/delta cells in the iIELs also bear CD8alpha/al

127 Thus, the failure to rearrange the TCR-gamma locus is due to a failure to initiate cleavage

128 IL-7 signal, we directly tested whether the TCR-gamma locus is accessible to cleavage by recombinant

129 c epsilon RI gamma-chain associates with the TCR-gamma delta complex in the absence of the zeta-chain

130 sed in IL-7R alpha -/- thymocytes, but these TCR-gamma genes, and Vgamma5, are not transcribed in thy

131 IL-7 was required for development of thymic TCR gamma delta cells, while peripheral IL-7 was suffici

132 a/BoyEg mice were found to be susceptible to TCR gamma delta+ cell mediated GVHD-induced lethality ch

133 ndogenous IL-2 gene expression in transgenic TCR gamma delta cells may be explained by subset-specifi

134 rts describing thymic differentiation in two TCR gamma delta transgenic mouse models have suggested t

135 rearranged, and sterile Vgamma4 and Vgamma6 TCR-gamma transcripts are expressed in IL-7R alpha -/- t

136 o studies have specifically examined whether TCR gamma/delta+ cells might be capable of eliminating B

137 nd J) sequences from an Ag-reactive TCR with TCR gamma junctional region sequences from an Ag-nonreac