ライフサイエンスコーパス: TCRbeta

1 sing the T cell antigen receptor beta-chain (TCRbeta).

2 ly as CD4- CD8- cells initiate expression of TCRbeta.

3 splacing cholesterol, which is known to bind TCRbeta.

4 the Vbeta1412-RS with the 3'Dbeta112-RS on a TCRbeta allele lacking Dbeta segments (the Jbeta1(M6) al

5 x on the Jbeta1(omega) allele, an endogenous TCRbeta allele that lacks the Dbeta2-Jbeta2 cluster, cre

6 Vbeta segments on unrearranged TCRbeta alleles are accessible in CD4(-)/CD8(-) (double-

7 ey might be regulated, we analyzed mice with TCRbeta alleles containing preassembled functional Vbeta

8 y, Vbeta14(Rep) recombination also occurs on TCRbeta alleles lacking endogenous Vbeta to DJbeta rearr

9 earrangements occur on only 5% of endogenous TCRbeta alleles, the Vbeta14(Rep) cassette underwent rea

10 machinery cooperate to help enforce IgH and TCRbeta allelic exclusion and indicate that control of V

11 ndogenous VbetaDJbetaCbeta genes can enforce TCRbeta allelic exclusion and reveal another mechanism t

12 To elucidate mechanisms that enforce TCRbeta allelic exclusion in such cells, we analyzed Vbe

13 ted grossly normal thymocyte development and TCRbeta allelic exclusion.

14 /lipid complex and high prevalence of Vbeta7 TCRbeta among the CD8(+) iNKT cells strongly point to a

15 In these individuals, T cell receptor beta (TCRbeta) analysis revealed that class II-restricted CD8(

16 We identified 15 TCRbeta and 4 TCRalpha antigen receptor sequences shared

17 TCRbeta and CD3 subunit protein sequence analyses among

18 ell as additional TCRs, consisting of the 2C TCRbeta and endogenous TCRalpha chains.

19 The mandatory requirement of TCRbeta and FcRgamma for FC function provides the first

20 elic expression and V-to-DJ recombination of TCRbeta and IgH genes.

21 tween alleles is more strictly regulated for TCRbeta and IgH loci, we evaluated the ability of ATM to

22 reduced D-to-J and V-to-DJ rearrangements of TCRbeta and IgH loci, whereas Rag2(C/C) mice show decrea

23 have been reported to increase the level of TCRbeta and Igmicro pre-mRNA, suggesting the hypothesis

24 b cells despite the absence of intracellular TCRbeta and reduced IL-7 signaling.

25 habeta T cell development, and by modulating TCRbeta and TCRalpha gene segment utilization.

26 In the present study, screening for TCRbeta and TCRalpha/delta translocations by FISH and li

27 of inter-chromosomal translocations between TCRbeta and TCRdelta D gene segments are also increased

28 D genes in the TCRbeta and TCRdelta loci are flanked by a 12RS and 23RS

29 eterodimer consisting of the TCR beta-chain (TCRbeta) and a 33-kDa protein, FCp33.

30 Incorporation of D segments into IgH, TCRbeta, and TCRdelta chains also contributes to junctio

31 re interpreted in conjunction with TCRgamma, TCRbeta, and TCRdelta rearrangements.

32 Our findings indicate that single TCRbeta are sufficient to confer on TCRalphabeta chains

33 This regulation is not a peculiarity of TCRbeta, as we identified many wild-type genes, includin

34 f gammadelta T cells and CD3(+)CD4(+)CD44(hi)TCRbeta(+)CCR6(+) natural Th17 (nTh17) cells, but not by

35 Cells were found to express TCRalpha, TCRbeta, CD152 (CTLA-4), CD154 (CD40L), T-bet, GATA-3, a

36 a population, BATF-expressing NKT cells are TCRbeta/CD3epsilon(low), but express normal levels of CD

37 imal domains of TCRalpha-CD3deltaepsilon and TCRbeta-CD3gammaepsilon.

38 s transient self-renewal (beta-selection) of TCRbeta(+) CD4(-)CD8(-) double-negative stage 3 (DN3) an

39 mice have greater numbers of IL-17-producing TCRbeta(+)CD4(+)cells in lymphoid organs and in the inte

40 We defined two precursor populations among TCRbeta(+)CD4(-)CD8(-) thymocytes by dependence on the k

41 metry subsequently confirmed the presence of TCRbeta(+) CD8(+) IL-17(+) T cells among tumor-infiltrat

42 ore, we observed that T cells with identical TCRbeta CDR3 nucleotide sequences were capable of recogn

43 ells expressing a conserved motif within the TCRbeta CDR3.

44 ution was evaluated by T-cell receptor beta (TCRbeta) CDR3 size spectratyping.

45 vels of IL-22 and larger numbers of IL-22(+) TCRbeta(+) cells and neutrophils in BALF.

46 has a restricted TCR repertoire with a fixed TCRbeta chain and a TCRalpha chain minilocus.

47 Using TCRbeta chain animals, we directly evaluate the extent o

48 Thus, transient modulation of the TCRbeta chain by H57-597 mAb exhibits potent, long-lasti

49 cholesterol and sphingomyelin binding to the TCRbeta chain causes TCR dimerization.

50 performed high-throughput sequencing of the TCRbeta chain complementarity-determining region 3 of li

51 The TCRbeta chain constant domain contains an unusually elon

52 TCRbeta chain diversity, measured by a novel technique,

53 d in mice solely transgenic for a rearranged TCRbeta chain gene.

54 e photocholesterol specifically binds to the TCRbeta chain in vivo.

55 lecular level, we forced the expression of a TCRbeta chain isolated from a peptide-independent allore

56 We purified these cells to generate TCRbeta chain libraries pre-enriched for target antigen

57 TCRbeta chain repertoire of peripheral alphabeta T cells

58 bled during thymocyte development influences TCRbeta chain selection and peripheral Vbeta repertoire.

59 an independent validation, we analysed 5,711 TCRbeta chain sequences from reactive CD4 T cells from 2

60 T cell repertoire as a whole, especially for TCRbeta chain sequences.

61 iple sclerosis, we used high-throughput deep TCRbeta chain sequencing to assess millions of individua

62 ll receptor (TCR) beta chain gene encoding a TCRbeta chain that forms a pre-TCR.

63 TCRalpha chains associated with a transgenic TCRbeta chain that the TRand CD25- CD4+ TCR repertoires

64 understanding of complementary TCRalpha and TCRbeta chain utilization is very limited for pathogen-

65 h peptide were established by the transgenic TCRbeta chain, and that this was compensated by addition

66 riple transgenic mice express the transgenic TCRbeta chain, but do not express a TCRalpha chain, and,

67 Using next-generation sequencing of the TCRbeta chain, clonally expanded T cells as a hallmark f

68 TCRalpha chain, which, when paired with the TCRbeta chain, forms a selectable alphabeta TCR.

69 or knowledge of variable region usage in the TCRbeta chain, resulting in a comprehensive, unbiased TC

70 transgene-encoded Vbeta5 chain and a revised TCRbeta chain.

71 phenotype and location in mice with a fixed TCRbeta chain.

72 nsferable feature of the peptide-independent TCRbeta chain.

73 g high throughput parallel sequencing of the TcRbeta chain.

74 xpressed transgene P14 T-cell receptor beta (TCRbeta) chain and CD8beta or did not (WT and KO mice, r

75 Dbeta or Jbeta genes, in place of an intact TCRbeta-chain and in association with TCRalpha.

76 ich is composed of a successfully rearranged TCRbeta-chain and the Pre-Talpha-chain.

77 T cell production in a manner independent of TCRbeta-chain expression.

78 hains in disease pathogenesis and the paired TCRbeta-chain remains unknown.

79 In this study, we used TCRbeta-chain transgenic mice to generate polyclonal nTr

80 encing approach, we determined TCRalpha- and TCRbeta-chain usage, as well as alphabetaTCR pairs expre

81 a tandem, multistep process to quantify rare TCRbeta-chain variable sequences of ASTs in large polycl

82 most cells that fail to produce a functional TCRbeta-chain will die instead of adopting the alternati

83 oded pairwise interactions.Rather, identical TCRbeta chains can have altered peptide-MHC (pMHC) bindi

84 Characterizing the TCRalpha and TCRbeta chains expressed by T cells responding to a give

85 ion of transcripts encoding the TCRalpha and TCRbeta chains from single cells.

86 Several TCRbeta chains paired with a transgenic TCRalpha chain t

87 riant TCRalpha chain and a restricted set of TCRbeta chains recognize structurally diverse antigens i

88 ed to isolate cDNA encoding the TCRalpha and TCRbeta chains that recognize the Kd54-68/I-Ab epitope.

89 sis with paired coexpression of TCRalpha and TCRbeta chains with single-cell resolution.

90 pervariable CDR3 regions on the TCRalpha and TCRbeta chains, and obtaining the paired sequences of th

91 When paired with certain Trbv TCRbeta chains, these TCRs recognize lipid antigens pres

92 n CD4(+)Vbeta5(-) T cells expressing revised TCRbeta chains.

93 ls sorted to remove cells bearing endogenous TCRbeta-chains can express newly generated TCRbeta molec

94 Sequence analysis of TCRbeta-chains of IGRP(+) cells reveals the repertoire c

95 with the prerearranged Vbeta in cell surface TCRbeta-chains were observed in Vbeta14(NT) and Vbeta8(T

96 lf of these lymphocytes expressed Vbeta14(+) TCRbeta-chains, even though similar steady-state levels

97 plenic alphabeta T cells expressed Vbeta8(+) TCRbeta-chains, only half of these lymphocytes expressed

98 cells expressed only Vbeta14(+) or Vbeta8(+) TCRbeta-chains, respectively, and lacked Vbeta rearrange

99 ctory aGVHD patients showed a more conserved TCRbeta clonal structure between different biopsy sites

100 g revealed oligoclonal expansion of specific TCRbeta clonotypes in CD8(+)PD-1(+) compared with CD8(+)

101 Furthermore, the most highly expanded TCRbeta clonotypes in the CD8(+) and the CD8(+)PD-1(+) p

102 ic traits of CD8(+) TILs and TCR beta chain (TCRbeta) clonotypic frequency in melanoma tumors to iden

103 We determined the genomic sequence of 244 TCRbeta coding junctions from 112 (63 male, 49 female) s

104 We performed deep sequencing of TCRbeta complementarity-determining region 3 (CDR3) regi

105 We found that T-cell receptor beta (TCRbeta) complementarity-determining region 3 repertoire

106 TCRbeta deep sequencing revealed oligoclonal expansion o

107 T-cell receptor beta (TCRbeta) deep sequencing revealed a striking contraction

108 When mutant SOD1 mice were bred onto a TCRbeta deficient background, disease progression was si

109 cient to induce disease in the resistant B10.TCRbeta(-)/(-)delta(-)/(-) strain.

110 inated by crossing B6x56R with CD4(-/)(-) or TCRbeta(-/-)delta(-/-) mice, and the effects on anti-dsD

111 mmadelta T cell homeostatic proliferation in TCRbeta(-/-)/delta(-/-) mice was not altered in the pres

112 sustained advantage following transfer into TCRbeta(-/-)/delta(-/-) mice, NK1.1(+) gammadelta T cell

113 ing of both immune receptor chains (VH+VL or TCRbeta/delta+TCRalpha/gamma) at the single-cell level f

114 is here using a series of public and private TCRbeta derived from autoimmune encephalomyelitis-associ

115 at patients with OS had marked reductions in TCRbeta diversity compared with control subjects, as exp

116 During this investigation, we focused on TCRbeta(+) DN thymocytes and found that there are at lea

117 least three functionally distinct subsets of TCRbeta(+) DN thymocytes: TCRbeta(+) DN3E, TCRbeta(+) DN

118 nterleukin 7 (IL-7) promotes the survival of TCRbeta(-) DN thymocytes by inducing expression of the p

119 Here we found that IL-7 signaled TCRbeta(+) DN3 and DN4 thymocytes to upregulate genes en

120 Likewise, expression of Bcl-2 in TCRbeta(+) DN3E cells was Gads independent, but Gads was

121 Survival and proliferation of TCRbeta(+) DN3E were independent of Gads, but survival a

122 istinct subsets of TCRbeta(+) DN thymocytes: TCRbeta(+) DN3E, TCRbeta(+) DN3L, and TCRbeta(+) DN4.

123 t of Gads, but survival and proliferation of TCRbeta(+) DN3L cells were Gads dependent.

124 t Gads was necessary for Bcl-2 expression in TCRbeta(+) DN3L cells.

125 f TCRbeta(+) DN thymocytes: TCRbeta(+) DN3E, TCRbeta(+) DN3L, and TCRbeta(+) DN4.

126 n TCRbeta(+) DN4 cells, but proliferation of TCRbeta(+) DN4 cells was Gads dependent.

127 cl-2 expression was not dependent on Gads in TCRbeta(+) DN4 cells, but proliferation of TCRbeta(+) DN

128 cytes: TCRbeta(+) DN3E, TCRbeta(+) DN3L, and TCRbeta(+) DN4.

129 We show that the TCRbeta enhancer (Ebeta) directs long-range chromatin op

130 -) mice were crossed with Bcl-xL-, Bcl2-, or TCRbeta-expressing transgenic mice, a modest level of co

131 e current study confirms that CD3epsilon and TCRbeta expression are present on the FC at the time of

132 Intracellular TCRbeta expression correlated with the up-regulation of

133 TCRbeta expression in CD4(-)CD8(-) double-negative (DN)

134 e form of Kuzbanian (dnKuz) leads to reduced TCRbeta expression in double-negative thymocytes and to

135 proceeds independent of the requirement for TCRbeta expression manifest in wild-type thymocytes, occ

136 At 5-8 wk of age, even in the absence of TCRbeta expression, CD4+ and CD4+CD8+ blasts appear spon

137 t only to a modest decrease in intracellular TCRbeta expression.

138 encies of ATM-deficient cells with biallelic TCRbeta expression.

139 and establishes that FcRgamma is part of the TCRbeta-FCp33 complex uniquely expressed on FC.

140 on and that FcRgamma coprecipitates with the TCRbeta-FCp33 heterodimer.

141 o results predicted by the accepted model of TCRbeta feedback inhibition, we found that expression of

142 haracterized by a systemic deficit in CD4(+) TCRbeta(+) Foxp3(+) CD25(+) T regulatory cells, increase

143 TCRbeta from patients with RAG mutations had less juncti

144 on of GATA3 leads predominantly to biallelic TCRbeta gene (Tcrb) recombination.

145 pression of either a preassembled functional TCRbeta gene (Vbeta1(NT)) or the prosurvival BCL2 protei

146 nmasks the Dbeta1 gene segment, and triggers TCRbeta gene assembly.

147 Rbeta rearrangements needed for a productive TCRbeta gene further increased frequencies of ATM-defici

148 impaired pre-TCR checkpoint with failure of TCRbeta gene rearrangement and increased apoptosis, resu

149 among T cell progenitors that have completed TCRbeta gene rearrangement without producing a functiona

150 Thymocytes undergoing TCRbeta gene rearrangements are maintained in a low or n

151 ination signal sequences (RSSs) flanking the TCRbeta gene segments.

152 d gender-specific V(D)J recombinase-mediated TCRbeta gene usage and coding joint processing at immune

153 TCRbeta gene usage studies of TRAG-3-specific CD4+ T cel

154 activated in immature thymocytes along with TcRbeta gene V(D)J recombination.

155 ression of a fully rearranged and functional TCRbeta gene, and most cells that fail to produce a func

156 is study, we demonstrate that a preassembled TCRbeta gene, but not a preassembled DbetaJbeta complex

157 use bone marrow that have not rearranged the TCRbeta gene; express a variety of genes associated with

158 typical B-cell marker, T-cell receptor beta (TCRbeta) gene rearrangement indicated a T-cell origin.

159 the CDR3 sequence in millions of rearranged TCRbeta genes from T cells of 2 adults.

160 s method involves sequencing of TCRalpha and TCRbeta genes, and amplifying functional genes character

161 go RAG-dependent rearrangement of endogenous TCRbeta genes, driving surface expression of novel TCRs.

162 xpression of both prerearranged TCRalpha and TCRbeta genes, indicating a critical role for TCR signal

163 phocytes with biallelic expression of IgH or TCRbeta genes.

164 T-cell receptor-beta (TCRbeta) genes naturally acquire premature termination c

165 ling and T-cell antigen receptor beta-chain (TCRbeta) genotyping on sequential genital skin biopsies,

166 vo administration of mAbs specific for mouse TCRbeta (H57-597), TCRalpha or CD3 promptly reduced the

167 t that the FCs, which express a unique FCp33-TCRbeta heterodimer in place of alphabetaTCR, permits HS

168 TCRbeta high-throughput sequencing in naive CTL of diffe

169 d developmentally specific V(D)J recombinase TCRbeta immune gene rearrangements and coding joint proc

170 that signaling pathways required to initiate TCRbeta-induced survival and proliferation are distinct

171 l 207-aa, 23-kDa protein, which includes the TCRbeta J2.7 region, and the entire C region.

172 The genomic organization of TCRbeta loci enables Vbeta-to-DJbeta2 rearrangements on

173 tes containing unrearranged or prerearranged TCRbeta loci.

174 and lacked Vbeta rearrangements on wild-type TCRbeta loci.

175 We have previously shown that of the two TCRbeta locus (Tcrb) D segments, Dbeta1 is flanked by an

176 -J recombination is outlined using the mouse TCRbeta locus as a model with frequent comparisons to th

177 lonality by using anchored RT-PCR of all the TCRbeta locus complementarity-determining region 3 (CDR3

178 ocyte development, molecular analyses of the TCRbeta locus in gammadelta cells and the TCRgamma and d

179 s) at which DNA cleavage is defective or how TCRbeta locus sequences contribute to these defects.

180 ps of DNA cleavage by the RAG proteins using TCRbeta locus V, D, and J RSS oligonucleotide substrates

181 sed to drive rearrangement of the endogenous TCRbeta locus, effecting cell rescue through the express

182 y mechanisms "beyond the 12/23 rule." In the TCRbeta locus, selective interactions between Rag protei

183 At the TCRbeta locus, the Ebeta enhancer and the Dbeta1 promote

184 e transcription from a Vbeta gene within the TCRbeta locus.

185 sors, accompanied by reduced numbers of both TCRbeta(low) immature single-positive CD8(+) cells and d

186 , resulting in the appearance of CD4(+)CD8(-)TCRbeta(-/low) thymocytes indistinguishable from DP thym

187 Prior studies indicated that public TCRbeta may be preferentially deployed in autoimmunity.

188 Our findings suggest that TCRbeta-mediated feedback inhibition of Vbeta14 rearrang

189 1(omega) alleles were similarly regulated by TCRbeta-mediated feedback regulation.

190 In the absence of alphabeta T cells, TCRbeta(-/-) mice exposed to S. rectivirgula for 4 wk ha

191 TCRbeta(-/-) mice were highly susceptible to T cell-medi

192 delta T cells by crossing LATY136F mice with TCRbeta(-/-) mice.

193 eta T cells (T-cell receptor beta-deficient [TCRbeta(-/-)] mice) and demyelination is gamma interfero

194 were reduced in spinal cords of SOD1(G93A) (TCRbeta-/-) mice.

195 We hypothesized that if these TCRbeta modulate the likelihood of a TCRalphabeta hetero

196 that three of the identified CMV-associated TCRbeta molecules bind CMV in vitro, and, moreover, we u

197 s TCRbeta-chains can express newly generated TCRbeta molecules in adoptive hosts.

198 NMD but instead reflects retention of PTC(+) TCRbeta mRNA in the nuclear fraction of cells.

199 Analysis of TCRbeta mRNA kinetics after either transcriptional repre

200 n-gamma-producing NKT-like (CD1d-independent TCRbeta+,NK1.1+ and/or DX5+) cells.

201 cers were identified as predominantly DX5(+) TCRbeta(+) NKT cells, and a comparable response could be

202 tion of the NMD factor UPF3b does not impair TCRbeta NMD, thereby distinguishing it from classical NM

203 ombination with UPF3b, also has no effect on TCRbeta NMD.

204 The frequency of TCRbeta nucleotide sequences was significantly higher in

205 Mice lacking alphabeta T cells (TCRbeta(null)) were completely deficient in their abilit

206 ease the frequencies of cells with biallelic TCRbeta or IgH expression while decreasing the frequency

207 ression or aberrant V-to-DJ rearrangement of TCRbeta or IgH loci in mice lacking ATM.

208 TCRbeta or TCRalphabeta transgenes failed to rescue DNMA

209 evels in mice lacking mast cells or T cells (TCRbeta(-/-) or Rag1(-/-)).

210 oglobulin heavy, T-cell receptor (TCR)alpha, TCRbeta, or TCRgamma chains expressed in a population of

211 public, but not private, disease-associated TCRbeta paired with endogenously rearranged TCRalpha end

212 t a hydrophobic patch created after TCRalpha-TCRbeta pairing has a role in maintaining the conformati

213 etains information about individual TCRalpha-TCRbeta pairs, TCRs of interest can be expressed and use

214 s us to track type II NTK cells by the GFP(+)TCRbeta(+) phenotype in the thymus and liver.

215 K/Akt pathway, which is required for pTalpha/TCRbeta (pre-TCR)-induced survival, differentiation, and

216 ich cells expressing functionally rearranged TCRbeta proliferate and differentiate into CD4(+)CD8(+)

217 ing TCRalphabeta thymocytes express a single TCRbeta protein, many thymocytes rearrange and express t

218 rearrangement without producing a functional TCRbeta protein.

219 e of the two alleles predicting a functional TCRbeta protein.

220 IgH and TCRbeta proteins drive proliferation of prolymphocytes t

221 two of six public, but none of five private TCRbeta provoked spontaneous early-onset autoimmunity in

222 ision, RAG reexpression mediates extrathymic TCRbeta rearrangement and results in a population of pos

223 This latter process is driven by TCRbeta rearrangement through RAG activity and results i

224 the Pten gene prior to the formation of the TCRbeta rearrangement, produced early in development.

225 ith clonal TCRalpha but no comparable clonal TCRbeta rearrangement, yielding events that would not no

226 production from progenitor cells undergoing TCRbeta rearrangement.

227 signaling to proceed even in the absence of TCRbeta rearrangement.

228 The few TCRbeta rearrangements detected were primarily out-of-fr

229 ily out-of-frame, suggesting that productive TCRbeta rearrangements diverted cells away from the gamm

230 gammadelta lineage occurred before complete TCRbeta rearrangements in most cases.

231 subject to feedback inhibition, we analyzed TCRbeta rearrangements in Vbeta14(Rep) mice containing a

232 DJbeta complex that decreases the number of TCRbeta rearrangements needed for a productive TCRbeta g

233 ifferentiation of thymocytes with productive TCRbeta rearrangements.

234 We find that total TCRbeta receptor diversity is at least 4-fold higher tha

235 ly devised assay, we characterized 48 unique TCRbeta recombination signal sequence (RSS) end insertio

236 ncing DNA double-strand breaks (DSBs) during TCRbeta recombination.

237 Dicer promotes survival of cells attempting TCRbeta recombination.

238 h levels of TCR excision circles, 2) complex TCRbeta repertoire diversity, and 3) proliferative respo

239 several primary recipients to increase their TCRbeta repertoire diversity.

240 showed early recovery of sjTREC numbers and TCRbeta repertoire diversity.

241 comprehensive evaluation of the naive CD8(+) TCRbeta repertoire in mice.

242 nt representation of a highly diverse public TCRbeta repertoire in the disease response.

243 Preselection-Tcrbeta repertoire is impaired and antigen-specific IgG

244 antify where limitations imposed on the Treg TCRbeta repertoire results in a population of Tregs that

245 In this study, we have employed deep TCRbeta repertoire sequencing with normalization based o

246 About 8-11% of the Treg TCRbeta repertoire was estimated to be the minimum requi

247 In this study, the Treg TCRbeta repertoire was reshaped and further narrowed.

248 ection of CD8alphaalpha precursors and their TCRbeta repertoire, but not in the maintenance of CD8alp

249 phaalpha precursors and limits their private TCRbeta repertoire.

250 echanisms for ensuring generation of diverse TCRbeta repertoires.

251 lices, namely, the three CD3epsilon-CD3gamma-TCRbeta segments and the five CD3epsilon-CD3delta-TCRalp

252 scovered a substantial number of public CDR3-TCRbeta segments that were identical in mice and humans.

253 ell development at the T cell receptor beta (TCRbeta) selection checkpoint and during positive select

254 Using a chimeric gene that contains TCRbeta sequences conferring this upregulatory response,

255 We identified TCRbeta sequences crucial for NIPS but found that NIPS i

256 sequencing was used to identify >18 x 10(6) TCRbeta sequences from the CNSs, periphery, and thymi of

257 can be used to capture and pair TCRalpha and TCRbeta sequences from total T-cell RNA, enabling revers

258 Mapping experiments revealed the identity of TCRbeta sequences that elicit a switch to UPF3b dependen

259 f TCRbeta transcripts, and we identified non-TCRbeta sequences that elicit NIPS.

260 sequencing data, we found that abundant CDR3-TCRbeta sequences were clustered within networks generat

261 ments of the naive CD8(+) T-cell repertoire, TCRbeta sequences with convergent features were (i) pres

262 ose CMV status from the resulting catalog of TCRbeta sequences with high specificity and sensitivity

263 ng a combination approach of high-throughput TCRbeta sequencing and multiparametric flow cytometry, w

264 cells was analyzed at various ages employing TCRbeta sequencing.

265 In this study, we use a fixed TCRbeta system to show that the TCR repertoire of the Fo

266 Although postrevision CD4(+)Vbeta5(-)TCRbeta(+) T cells accumulate with age in Vbeta5 transge

267 results in the appearance of CD4(+)Vbeta5(-)TCRbeta(+) T cells, coinciding with Rag1, Rag2, and TdT

268 n occur at three T-cell receptor (TCR) loci: TCRbeta, TCRgamma and TCRdelta.

269 forced expression of functionally rearranged TCRbeta, TCRgamma, and TCRdelta chains by means of trans

270 t are completely devoid of T cells (B6.129P2-Tcrbeta(tm1Mom) Tcrdelta(tm1Mom)/J) show protection agai

271 lar TCR-beta protein and decreased levels of tcrbeta transcript are expressed by T cells cultured in

272 sites up-regulated an alternatively spliced TCRbeta transcript that skipped the mutations independen

273 t up-regulation of the alternatively spliced TCRbeta transcript.

274 reiterated GC motifs contribute to germline TCRbeta transcription through binding of KLF5 and other

275 e to lineage-specific regulation of germline TCRbeta transcription.

276 PTC-bearing TCRbeta transcripts are dramatically down-regulated to p

277 smic fraction mRNA ratio that results in few TCRbeta transcripts escaping to the cytoplasmic fraction

278 d that NIPS is not exclusively a property of TCRbeta transcripts, and we identified non-TCRbeta seque

279 dominant clone varied between 11% and 99% of TCRbeta transcripts.

280 row and spleen cells expressing TCRalpha and TCRbeta transgenes that recognize CD1d.

281 repertoire breadth to a non-self-antigen, a TCRbeta transgenic mouse model (EF4.1) expressing a limi

282 the TCR repertoire of thymic T(reg) cells in TCRbeta-transgenic mice was diverse and was more similar

283 pertoire of T(reg) cells in Foxp3-sufficient TCRbeta-transgenic mice, suggesting that these self-reac

284 cluding radial chromosome translocations and TCRbeta translocations, compared with cells lacking Atm

285 urther insight into this question, we used a TCRbeta transmembrane domain mutant model that is defect

286 Here we show that cholesterol bound to the TCRbeta transmembrane region keeps the TCR in a resting,

287 ffector memory-RA(+) subsets with restricted TCRbeta usage and nearly monoclonal CDR3 containing nove

288 that FOXP3(+) Tregs possess highly exclusive TCRbeta usage from conventional T cells, in blood, and a

289 tepwise assembly and subsequent selection of TCRbeta V region exons during thymocyte development.

290 ombined the DNA of one T cell receptor beta (TCRbeta) V-to-DJ-joined allele in a functional configura

291 d/or TCRgamma rearrangements but no complete TCRbeta variable diversity joining rearrangement in surf

292 orce selective D gene incorporation into the TCRbeta variable domain in the absence of other nuclear

293 ghly biased, with a predominant usage of the TCRbeta variable gene 2 (TRBV2) in vaccinees as well as

294 TCRbeta variable gene segments remained largely in germl

295 ally diverse TCR repertoires, with different TCRbeta variable regions and with high amino acid divers

296 can assemble with both chicken TCRalpha and TCRbeta via conserved polar transmembrane sites.

297 nd, consistently, public, disease-associated TCRbeta were observed to be commonly oligoclonal.

298 A tilting of the pre-TCRbeta when bound to the pMHC ligand recognition surfac

299 drive oncogene expression differ markedly in TCRbeta (which are exclusively enhancer driven) and TCRa

300 Using T-cell receptor-beta (TCRbeta), which naturally acquires PTCs at high frequenc