ライフサイエンスコーパス: TFIIA

1 TFIIA (tau+gamma) supported basal and activated transcri

2 TFIIA and TATA-binding protein (TBP) associate directly

3 TFIIA antagonizes these TBP repressors but may be effect

4 TFIIA bridges the TBP-TATA complex with lobe B of TFIID.

5 TFIIA induced a rapid dissociation of TFIID dimers, allo

6 TFIIA interacts with TFIID via association with TATA bin

7 TFIIA is encoded by two genes (alphabeta and gamma) that

8 TFIIA phosphorylation had little effect on the TFIIA.TBP

9 TFIIA plays a central role in loading TBP and its multis

10 TFIIA significantly enhanced the kinetic stability of TB

11 TFIIA was dispensable for ICP4 activation of a late prom

12 TFIIA-gammaY65A is defective for binding to the beta-she

13 uence within a reconstituted nucleosome in a TFIIA-dependent manner.

14 TFIIA allows for the production of an active TFIIA-like general transcription factor throughout oogen

15 ATA-binding protein (TBP), which then allows TFIIA and TFIIB to be recruited.

16 Although TFIIA gamma interacts with Runx2, it does not activate R

17 ding was not significantly changed, although TFIIA did increase the fraction of DNA molecules bound b

18 ma), formed the TFIIA-TBP-TATA DNA (T-A) and TFIIA-TFIIB-TBP-TATA DNA (TAB) complexes indistinguishab

19 Here we characterize endogenous ALF and TFIIA activities in the African clawed frog Xenopus laev

20 y regulated reciprocal expression of ALF and TFIIA allows for the production of an active TFIIA-like

21 ced maturation, ALF activity disappears, and TFIIA activity is restored.

22 DNA bending is affected by TATA sequence and TFIIA.

23 ealed that the interaction between TAF11 and TFIIA involves two distinct regions of TAF11: the highly

24 t that Swi/Snf stimulates binding of TBP and TFIIA.

25 n at a step involving DNA binding by TBP and TFIIA.

26 e effects of transcription factors TFIIB and TFIIA reported previously.

27 onal general transcription factors TFIIB and TFIIA.

28 llowing findings: (1) In one step, TFIID and TFIIA assemble at the promoter independently of holoenzy

29 und that while promoter binding of TFIID and TFIIA is stable, promoter binding by TFIIB is highly tra

30 omponents tested without affecting TFIID and TFIIA recruitment.

31 ith TFIIA, increase recruitment of TFIID and TFIIA to the promoter, and promote isomerization of the

32 mation by enhancing the binding of TFIID and TFIIA, whereas Gal4-VP16 could enhance the recruitment o

33 embly of a complex containing only TFIID and TFIIA.

34 teristic contained general factors TFIID and TFIIA.

35 ies generated with purified Rap1, TFIID, and TFIIA on RPG enhancer-promoter DNA indicate that Rap1-TF

36 Because TFIIA is known to stabilize the binding of both TATA bin

37 ion-steric exclusion-for competition between TFIIA and NC2.

38 contact or creating a second contact between TFIIA and TBP that was not visible in the crystal struct

39 role for TAF40 as a bridging factor between TFIIA and TFIID.

40 that loss of functional interaction between TFIIA and TAF40 results in conditional growth phenotypes

41 further explore the functional link between TFIIA and TAF11, the toa2-I27K allele was utilized in a

42 ocate the TATA-binding protein (TBP) between TFIIA and TFIIB at the top of the cavity that most likel

43 Moreover, TAF11 imparts changes to both TFIIA-DNA and TBP-DNA contacts in the context of promote

44 nts of enhancer-promoter DNA and TFIID-bound TFIIA.

45 transcription, both of which are enhanced by TFIIA.

46 , such as INO1, was dramatically impaired by TFIIA depletion, activation of other genes, such as CUP1

47 ween these structural states is modulated by TFIIA, as the presence of TFIIA and promoter DNA facilit

48 ly to promoter DNA in a manner stabilized by TFIIA, and these complexes can be analyzed by native gel

49 f these genetic defects can be suppressed by TFIIA overexpression, strongly suggesting a role for yFA

50 The stabilized binding of TFIID by TFIIA may in turn allow ICP4 to more efficiently activat

51 As we demonstrate, the TRF4/SNAP(c)/TFIIA complex binds specifically to the SL RNA gene prom

52 osteoblast cells showed that in intact cells TFIIA gamma is recruited to the region of the osteocalci

53 ding RNA polymerase II and protein complexes TFIIA, TFIIB, TFIID (or TBP), TFIIE, TFIIF, TFIIH and TF

54 Consistently, TFIIA and yTAF145 bind competitively to TBP.

55 tiation complexes (partial PICs), containing TFIIA, TFIID (and/or SAGA), TFIIB, TFIIE, and TFIIF.

56 midated, but V96F substitution of deamidated TFIIA amino acid residues 91-100 stimulated IL-2 release

57 report a requirement for Taspase 1-dependent TFIIA proteolytic processing in the mouse testis to enab

58 ucted yeast strains to conditionally deplete TFIIA levels prior to gene activation.

59 Dephosphorylation of yeast-derived TFIIA reduced DNA binding activity, and recombinant TFII

60 rly to the DQ2-gamma-I-gliadin epitope (i.e. TFIIA, FOXJ2 and IgD; mean BestFit quality score=40 vers

61 t3, or near residues known to contact either TFIIA or Brf1 (in TFIIIB).

62 promises its ability to interact with either TFIIA or Brf1.

63 ression of TOA1 and TOA2, the genes encoding TFIIA.

64 cements in only one of these regions enhance TFIIA-TBP-DNA complex formation in vitro, suggesting tha

65 s, we found that ICP4 was able to facilitate TFIIA stabilized binding of TBP to the TATA box of the e

66 tein (TBP) with general transcription factor TFIIA and transcriptional repressor NC2.

67 cleavage of the general transcription factor TFIIA ensures proper coordination of rapid cell prolifer

68 irement for the general transcription factor TFIIA in vitro.

69 the RNA Pol II general transcription factor TFIIA.

70 eta) subunit of general transcription factor TFIIA.

71 cturally related to the transcription factor TFIIA.

72 or Mot1 and the general transcription factor TFIIA.

73 ts and with the general transcription factor TFIIA.

74 y shows that a general transcription factor, TFIIA gamma, facilitates osteoblast-specific gene expres

75 inding another general transcription factor, TFIIA.

76 The general factors TFIIA and TFIIB each increase the rotational and transla

77 similarities between Bdp1 and Pol II factors TFIIA and TFIIF, and unravels essential interactions wit

78 General transcription factors TFIIA and TFIIB bound glutathione S-transferase-TRP in s

79 General transcription factors TFIIA, -B, -D, -E, -F, -H were located near the downstre

80 Finally, TFIIA gamma significantly prevents ATF4 degradation.

81 o identified essential points of contact for TFIIA and Rap1 within the Rap1 binding domain of the Taf

82 re, we demonstrate an essential function for TFIIA in TIC- and TAFII-dependent basal transcription fr

83 s factor serves as an active replacement for TFIIA.

84 acids 258-286) was found to be required for TFIIA gamma binding.

85 A role for TFIIA in distinguishing between TATAAA and CATAAA in viv

86 ate promoter because ICP4 can substitute for TFIIA's ability to stabilize the binding of TFIID to the

87 nase domain of TAF(II)250 was sufficient for TFIIA phosphorylation, and this activity was inhibited b

88 nscription) counteracted by addition of free TFIIA.

89 A DNA (TAB) complexes indistinguishably from TFIIA (alphabeta+gamma).

90 Furthermore, TFIIA gamma together with ATF4 and Runx2 stimulates oste

91 fied general transcription factor IIA gamma (TFIIA gamma) as a Runx2-interacting factor in a yeast tw

92 pase1) cleaves a ubiquitously expressed GTF (TFIIA) to enable tissue-specific (testis) transcription,

93 How TFIIA and TFIID communicate is not well understood.

94 However, TFIIA (tau+gamma) was reduced relative to TFIIA (alphabe

95 ant human (rh)TRF2 nor the native flag.hTRF2-TFIIA complex is able to replace TBP or TFIID in basal o

96 Instead, rhTRF2, but not the flag.hTRF2-TFIIA complex, moderately inhibits basal or activated tr

97 Neither rhTRF2 nor flag.hTRF2-TFIIA has any effect on the repression of TFIID-mediated

98 partially conserved between yeast and human TFIIA large subunits.

99 Our results indicate that human TFIIA decreases the angle to which human TBP bends conse

100 We now show that human TFIIA is phosphorylated in vivo on serine residues that

101 r factors such as SP1, TFIIB, polymerase II, TFIIA, or p65, can be acetylated by CBP/p300 HAT domain.

102 nd TBP, TRF2 binds transcription factor IIA (TFIIA) and TFIIB and appears to be part of a larger prot

103 some then recruits transcription factor IIA (TFIIA) and TFIID to the promoter to form the DA complex.

104 The general transcription factor IIA (TFIIA) binds to the TATA binding protein (TBP) and is es

105 The general transcription factor IIA (TFIIA) forms a complex with TFIID at the TATA promoter e

106 Transcription factor IIA (TFIIA) is a positive acting general factor that contacts

107 The general transcription factor IIA (TFIIA) stimulates RNA polymerase II-specific transcripti

108 overexpression of transcription factor IIA (TFIIA), suggesting that histone acetylation by Gcn5 can

109 of SNAP(c) and of transcription factor IIA (TFIIA).

110 ly) of the general transcription factor IIA (TFIIA).

111 n mutation of serine residues 316 and 321 in TFIIA alphabeta reduced TFIIA phosphorylation significan

112 (3) Extracts deficient in TFIIA activity showed reduced assembly of all PIC compon

113 an open conformation at the lobe involved in TFIIA and putative activator interactions.

114 Instead, TFIIA gamma binds to and activates ATF4.

115 Interestingly, TFIIA increased the calculated bend angle and kinetic st

116 repression was observed in reactions lacking TFIIA and with recombinant TATA-binding protein in place

117 ChIP analyses determined that noncleaved TFIIA accumulates at the p16Ink4a and p19Arf promoters t

118 four serine residues reduced the ability of TFIIA to stimulate transcription in transient transfecti

119 tivation in vivo can occur in the absence of TFIIA.

120 ation of the late promoter in the absence of TFIIA.

121 The additional activities of TFIIA were required to stabilize the binding of TFIID to

122 er and requires the additional activities of TFIIA.

123 -TFIIA-DNA complex formation and addition of TFIIA overcame TRP-mediated transcription repression.

124 a genetic screen, we isolated a new class of TFIIA mutants and identified three regions on TFIIA that

125 nuclear extracts that have been depleted of TFIIA.

126 However, depletion of TFIIA causes a general two- to threefold decrease of tra

127 man cell-free transcription system devoid of TFIIA and Mediator.

128 ed FRET (gelFRET) to determine the effect of TFIIA on the conformation of the DNA in TBP/TATA complex

129 ryotic core promoter involves the effects of TFIIA on the interaction between TATA-binding protein (T

130 ctivator, suggesting that these two forms of TFIIA have activator specificity.

131 We found that fusion of TFIIA to TBP dramatically increases transcription from C

132 Moreover, fusion of TFIIA to TBP specifically restores activity from these t

133 However, the recent identification of TFIIA as part of a protein complex essential for RNA pol

134 To determine the relative importance of TFIIA in the regulation of different genes, we construct

135 iation complexes can contain Mot1 instead of TFIIA in vivo.

136 Molecular modeling of TFIIA[V96F] peptide points to an important juxtaposition

137 n cleavage reagents positioned on the NTD of TFIIA and assembled in PICs identified the SAGA subunit

138 Overexpression of TFIIA gamma increases levels of ATF4 protein.

139 In contrast, overexpression of TFIIA inhibits transcription, indicating that the somati

140 ities can be suppressed by overexpression of TFIIA, TBP, or Nhp6, suggesting that these genes facilit

141 However, phosphorylation of TFIIA containing a gamma subunit Y65A mutation strongly

142 Phosphorylation of TFIIA could therefore be an important mechanism of trans

143 ts TBP-DNA binding, whereas preincubation of TFIIA with TBP prevents this inhibition.

144 es is modulated by TFIIA, as the presence of TFIIA and promoter DNA facilitates the formation of a re

145 Moreover, in the presence of TFIIA, the extent of DNA bending was not significantly c

146 nt TATA boxes in the absence and presence of TFIIA.

147 g protein (TBP), blocking the recruitment of TFIIA and TFIIB, and thereby inhibiting preinitiation co

148 ly to be similar or identical to the role of TFIIA in somatic cells.

149 To investigate the role of TFIIA in TFIID recruitment in vivo, we exploited a class

150 The physiological role of TFIIA was investigated by analyzing transcription in a y

151 BP-DNA complexes, since relevant segments of TFIIA and NC2 were not present in truncated TFIIA and NC

152 IC, such as TFIIE and TFIIH, and segments of TFIIA, TFIIB and TFIIF.

153 Small interfering RNA silencing of TFIIA gamma markedly reduces levels of endogenous ATF4 p

154 l were not observed in crystal structures of TFIIA-TBP-DNA and NC2-TBP-DNA complexes, since relevant

155 dentified a mutation in the small subunit of TFIIA (toa2-I27K) that is defective for interaction with

156 Mutational analyses of the Toa2 subunit of TFIIA indicate that loss of functional interaction betwe

157 Mutations in the TOA2 subunit of TFIIA that disrupt TBP-TFIIA complex formation in vitro

158 AA in vivo was tested by fusing a subunit of TFIIA to TBP.

159 te yeast cells of Toa1, the large subunit of TFIIA, to <1% of the wild-type level.

160 terpart of the large (alpha/beta) subunit of TFIIA.

161 PlotSimilarity alignment of TFIIAs from eight species revealed subthreshold similari

162 FIIA mutants and identified three regions on TFIIA that are likely to be involved in TBP recruitment

163 The presence of ALF or TFIIA did not significantly alter the ability of TBP to

164 n be attributed to binding either the DNA or TFIIA recognition sites.

165 spt16 mutation are enhanced by either TBP or TFIIA mutants.

166 Unlike wild-type TBP or TFIIA, the N2-1 derivative does not activate transcripti

167 mutation with TATA binding protein (TBP) or TFIIA defects.

168 ession after the establishment of a partial (TFIIA/TFIID) preinitiation complex (PIC).

169 Phosphorylated TFIIA was 30-fold more efficient in forming a stable com

170 iated factor 250 (TAF(II)250) phosphorylated TFIIA on the beta subunit.

171 istent with the existence of a critical Rap1-TFIIA-TFIID interaction network.

172 educed DNA binding activity, and recombinant TFIIA could be stimulated by in vitro phosphorylation wi

173 a system consisting of purified recombinant TFIIA, TFIIB, TFIIE, TFIIF, CREB, and Tax, along with hi

174 idues 316 and 321 in TFIIA alphabeta reduced TFIIA phosphorylation significantly in vivo.

175 in this organism, and it completely replaces TFIIA in immature oocytes.

176 ficantly in the degree to which they require TFIIA for activation.

177 to the promoter in a step that also requires TFIIA and confirm that recruitment of the PIC by E2F is

178 ors and general cofactors, such as TAF(II)s, TFIIA, Mediator, and PC4, are not required for E2-mediat

179 TA-containing c-fos promoter by sequestering TFIIA.

180 er-proximal region of the germ cell-specific TFIIA alpha/beta-like factor (ALF) gene.

181 ized properties of ALF, a germ cell-specific TFIIA-like factor.

182 ystematic mutagenesis to define how specific TFIIA contacts contribute to these processes.

183 ATAAA and TATAAG are unable to form a stable TFIIA-TBP-DNA complex in vitro.

184 ma subunit Y65A mutation strongly stimulated TFIIA.TBP.TATA complex formation.

185 ranscription modulation, since it stimulates TFIIA-TBP association, enhances high-level transcription

186 at phosphorylation of yeast TFIIA stimulates TFIIA.TBP.TATA complex formation and transcription activ

187 onstituted with the testis-specific subunit, TFIIA (tau+gamma), formed the TFIIA-TBP-TATA DNA (T-A) a

188 Interestingly, such TFIIA-depleted cells are essentially unaffected for acti

189 ding the molecular organization of the TAF11-TFIIA interaction and define a mechanistic role for this

190 The TATA-binding protein (TBP), TFIIA, and TFIIB interact with promoter DNA to form a co

191 factors such as TATA binding protein (TBP), TFIIA, and TFIIB, but not with occupancy by TBP-associat

192 interaction with TATA-binding protein (TBP), TFIIA, and TFIIB.

193 s for the general transcription factors TBP, TFIIA and IIB determined by TIRF-PBM are similar to thos

194 es of the general transcription factors TBP, TFIIA, TFIIB and TFIIH and showed that these factors are

195 to study the stepwise assembly of human TBP, TFIIA, TFIIB, Pol II, TFIIF, TFIIE and TFIIH onto promot

196 tified extremely divergent orthologs of TBP, TFIIA, TFIIB, and TFIIH which, together with the small n

197 ffected in the initial formation of the TBP, TFIIA-TBP, or TFIIB-TBP complexes.

198 and that Nhp6 stimulates formation of a TBP-TFIIA-DNA complex.

199 n the TOA2 subunit of TFIIA that disrupt TBP-TFIIA complex formation in vitro are also synthetically

200 strongly suggesting a role for yFACT in TBP-TFIIA complex formation in vivo.

201 sults suggesting importance of TBP H2 in TBP-TFIIA interactions and TBP-NC2 interactions, and provide

202 reincubation of TRP with TFIIA inhibited TBP-TFIIA-DNA complex formation and addition of TFIIA overca

203 e find that the ability to stabilize the TBP-TFIIA complex on the hsp70 and c-fos TATA elements, and

204 these genes facilitate formation of the TBP-TFIIA-DNA complex.

205 coactivators stimulate formation of the TBP-TFIIA-DNA complex.

206 cription by promoting the formation of a TBP/TFIIA complex.

207 Instead, a highly stable, preloaded TBP/TFIIA "pioneer" complex primes the rapid initiation of H

208 litates assembly of a complex containing TBP:TFIIA:TFIIB, which lacks TAFs, and provides a mechanism

209 me mutation lowered the stability of the TBP:TFIIA complex on the DNA.

210 nterfere with different interactions (TFIIB, TFIIA, or the TATA box) and a TFIIB mutant defective for

211 pies promoters with TBP, but not with TFIIB, TFIIA, or Pol II when cells are grown in normal conditio

212 echanism for how interactions between TFIID, TFIIA, and Rap1 contribute to the high rate of transcrip

213 ility to recruit a complex containing TFIID, TFIIA and TFIIB.

214 and the general transcription factors TFIID, TFIIA, TFIIB, TFIIF, TFIIE, and TFIIH.

215 e that includes transcription factors TFIID, TFIIA, TFIIH, TFIIE and Mediator.

216 VP16 could enhance the recruitment of TFIID, TFIIA, and holoenzyme.

217 erarchal promoter-specific binding of TFIID, TFIIA, and TFIIB.

218 on factors rapidly bind promoter-bound TFIID-TFIIA, after which complexes undergo a slow isomerizatio

219 , and TFIIF assemble on promoter-bound TFIID-TFIIA.

220 de interference with either TBP-DNA or TFIID-TFIIA-DNA contacts both upstream and downstream of the T

221 ement (DPE) promoter motifs within the TFIID-TFIIA-DNA structure.

222 caused a conformational change in the TFIID-TFIIA-promoter complex as assessed by DNase I footprinti

223 oter, and promote isomerization of the TFIID-TFIIA-TATA complex.

224 nt of TFIIF and RNA polymerase II to a TFIID/TFIIA/TFIIB/promoter complex dictates the rate of preini

225 wise assembly pathway is blocked after TFIID/TFIIA binding.

226 ning the activator GAL4-VP16, Med, and TFIID/TFIIA (DA) recruits pol II and the remaining GTFs to a m

227 pendent variations in retention of the TFIID:TFIIA complex after release of the polymerase could be a

228 The ability of the TFIID:TFIIA complex to complete assembly rapidly was reduced b

229 Immunoprecipitation assays confirmed that TFIIA gamma interacts with Runx2 in osteoblasts and when

230 present a refined model for the effect that TFIIA has on DNA conformation that takes into account po

231 Using purified proteins, it was found that TFIIA and Spt8 do not stably bind to each other, but rat

232 reduced by a similar extent, indicating that TFIIA function is largely nonselective for activators.

233 rologous DNA binding domain, indicating that TFIIA is important for transcription even in the absence

234 us activators and promoters, indicating that TFIIA phosphorylation is required globally for optimal f

235 ing noncleavable TASP1 targets revealed that TFIIA is the principal TASP1 substrate that orchestrates

236 Our data show that TFIIA induces a conformational change within the TBP/TAT

237 Here, we show that TFIIA promotes the dissociation of TBP dimers directly a

238 These and other results suggest that TFIIA acts at an early step by enhancing the stable recr

239 These data suggest that TFIIA facilitates DNA binding by TBP in vivo, that TFIIA

240 Further, they suggest that TFIIA function may not be strictly related to the recrui

241 These results suggest that TFIIA is a primary target of TRP transcription inhibitio

242 Biochemical experiments suggest that TFIIA is important in the response to transcriptional ac

243 Collectively, these data suggest that TFIIA may be dispensable for ICP4 activation of the wild

244 These results suggest that TFIIA phosphorylation is important for strengthening the

245 Taken together, these results suggest that TFIIA plays an important role in both activator-dependen

246 We have verified that TFIIA is required for RPG transcription in vivo and in v

247 facilitates DNA binding by TBP in vivo, that TFIIA may be regulated by factors that target distinct r

248 The TFIIA complex reconstituted with the testis-specific sub

249 The TFIIA-related protein (TFIIAtau) was cloned from a testi

250 th these findings, mutations in Spt8 and the TFIIA NTD interact genetically.

251 In striking contrast, the TFIIA-TBP-DNA complex is significantly less stable on th

252 reduced by mutations in TRP that disrupt the TFIIA binding surface but not by mutations that disrupt

253 itution mutation at W285, which disrupts the TFIIA-TBP interface.

254 cific subunit, TFIIA (tau+gamma), formed the TFIIA-TBP-TATA DNA (T-A) and TFIIA-TFIIB-TBP-TATA DNA (T

255 at TBP H2 can be crosslinked to TFIIA in the TFIIA-TBP-DNA complex and in higher order transcription-

256 e crosslink to the 'connector' region of the TFIIA alpha/beta subunit (TFIIAalpha/beta).

257 n in vitro, and the positive function of the TFIIA NTD is largely mediated through Spt8.

258 OsTFIIAgamma5 encoding a second form of the TFIIA small subunit on chromosome 5 of rice.

259 e results indicate that the stability of the TFIIA-TBP complex depends strongly on the sequence of th

260 Modulation of the TFIIA-TBP interaction is a likely target of transcriptio

261 hance the formation and stabilization of the TFIIA-TBP-DNA complex.

262 IIA phosphorylation had little effect on the TFIIA.TBP.TATA complex in electrophoretic mobility shift

263 and that this phosphorylation stabilizes the TFIIA-TBP-DNA complex and is required for high-level tra

264 orylation is important for strengthening the TFIIA.TBP contact or creating a second contact between T

265 These results demonstrate that the TFIIA-TAF40 interaction is important in vivo and indicat

266 ce of the core promoter element and that the TFIIA-TBP complex plays an important function in recogni

267 omoters indicate that the sensitivity to the TFIIA-TBP interaction can map either to the upstream or

268 Thus, TFIIA competes with TAFII250 for access to TBP and alter

269 In vitro, purified TLF-TFIIA binds directly to the same NF1 promoter fragment t

270 have found that TBP H2 can be crosslinked to TFIIA in the TFIIA-TBP-DNA complex and in higher order t

271 r, TFIIA (tau+gamma) was reduced relative to TFIIA (alphabeta+gamma) for stimulating transcription wi

272 TFIIA and NC2 were not present in truncated TFIIA and NC2 derivatives used for crystallization.

273 as human chorionic gonadotrophin, ubiquitin, TFIIA, guanine nucleotide-binding protein (beta-subunit)

274 Although the unprocessed TFIIA still complexes with TRF2, this complex is impaire

275 We also used TFIIA mutants that eliminated in vitro interactions with

276 These viable TFIIA mutants were lethal in strains lacking Gcn5, Swi2,

277 e results suggest a novel mechanism by which TFIIA assists in regulating gene expression.

278 the structure of human TFIID in complex with TFIIA and core promoter DNA, determined by single-partic

279 rase II machinery that works in concert with TFIIA (IIA) and TFIIB (IIB) to assemble initiation compl

280 hTAF(II)250) to specifically cooperate with TFIIA to relieve TAF(II)250-mediated repression of TBP b

281 res with higher order complex formation with TFIIA and/or TFIIB.

282 because activation domains can interact with TFIIA, increase recruitment of TFIID and TFIIA to the pr

283 no acid substitution mutants interacted with TFIIA-TFIID and CBP indistinguishably from the wild type

284 mutant (N2-1) defective for interacting with TFIIA.

285 TAF11 allele defective for interaction with TFIIA exhibit conditional growth phenotypes and defects

286 idues were required for Zta interaction with TFIIA-TFIID and the CREB-binding protein (CBP) and for s

287 s surface is important for interactions with TFIIA and Brf1, TBP interactions with these two factors

288 , we show that T antigen also interacts with TFIIA in vitro.

289 y RBP is the same domain that interacts with TFIIA, but is disparate from the domain that interacts w

290 ponds to the TBP surface that interacts with TFIIA.

291 nd elongation-competent Pol II, but not with TFIIA.

292 t activators in reactions reconstituted with TFIIA-depleted nuclear extracts.

293 F40 interacts directly and specifically with TFIIA.

294 a trilobed, horseshoe-shaped structure, with TFIIA and TFIIB bound on opposite lobes and flanking a c

295 ivator OCA-B nor the interaction of TBP with TFIIA.

296 Preincubation of TRP with TFIIA inhibited TBP-TFIIA-DNA complex formation and addi

297 f TOA1 and TOA2, the genes that encode yeast TFIIA, overcomes the activation defects caused by the TB

298 eviously unknown yet critical role for yeast TFIIA in the integration of activator-TFIID contacts wit

299 we have found that phosphorylation of yeast TFIIA stimulates TFIIA.TBP.TATA complex formation and tr

300 t here that Toa1, the large subunit of yeast TFIIA, is phosphorylated in vivo and that this phosphory