ライフサイエンスコーパス: TFIIIB

1 TFIIIB and TFIIIC are multi-subunit factors required for

2 TFIIIB assembled with certain deletion mutants of its Br

3 TFIIIB in turn directs RNA polymerase III binding and in

4 TFIIIB is a multisubunit complex that includes the TATA-

5 TFIIIB is a target of tumor suppressors, including PTEN,

6 TFIIIB is brought to a site centered approximately 35 bp

7 TFIIIB is composed of three subunits, the TATA binding p

8 TFIIIB is required for recruitment of Pol III and to pro

9 TFIIIB recruits its polymerase to the promoter and subse

10 TFIIIB transcriptional activity and TATA-box binding act

11 TFIIIB, the central transcription initiation factor of t

12 TFIIIB, the initiation factor for transcription by RNA p

13 TFIIIB, the RNA polymerase III-recruiting factor of Sacc

14 TFIIIB, which is brought to RNA polymerase III-transcrib

15 TFIIIB-DNA complexes in yeast comprise the TATA-binding

16 Ks coordinately regulate expression of all 3 TFIIIB subunits.

17 lysis revealed that dTAFIII105, present in a TFIIIB fraction, directly interacts with TATA-binding pr

18 ernal promoter elements, and contains TBP, a TFIIIB" homologue (TFIIIB150), and a BRF1 homologue (TFI

19 Erk and c-Myc, which directly activate TFIIIB in proliferating fibroblasts, also induce pol III

20 reases the effective concentration of active TFIIIB molecules.

21 IB", whereas two distinct TFIIIB activities, TFIIIB-alpha and TFIIIB-beta, have been described in hum

22 In addition, TFIIIB is bound and activated by several oncogenic prote

23 selective reduction in the occupancy of all TFIIIB subunits on tRNA(Leu) genes, whereas prolonged PT

24 distinct TFIIIB activities, TFIIIB-alpha and TFIIIB-beta, have been described in human cells.

25 in (TBP), TFIIB-related factor 1 (BRF1), and TFIIIB", whereas two distinct TFIIIB activities, TFIIIB-

26 anscription machinery, including SNAP(C) and TFIIIB.

27 ments for transcription factor (TF) IIIC and TFIIIB in Ty3 integration into the two initiation sites

28 ltaG degrees = -12.10 +/- 0.12 kcal/mol) and TFIIIB-DNA (DeltaG degrees = -11.90 +/- 0.14 kcal/mol) c

29 ain in directing Rb-mediated repression, and TFIIIB is the principal target of that activity.

30 plexes [Transcription Factor IID (TFIID) and TFIIIB] were also found to diminish its affinity for the

31 (TBP)-TAF complexes, namely, SL1, TFIID, and TFIIIB.

32 nd its two transcription factors, TFIIIC and TFIIIB, on a subset of pol III genes.

33 o transcription factor complexes, TFIIIC and TFIIIB.

34 hemical nuclease footprinting of TFIIIC- and TFIIIB-TFIIIC-DNA complexes reveals that Nhp6a markedly

35 d the differential in both transcription and TFIIIB complex assembly is observed at saturating levels

36 nt for the subsequent recruitment of another TFIIIB subunit, Bdp1, implying that repositioning of Brf

37 to a significant extent by the level of any TFIIIB subunit.

38 Because TFIIIB can bind to the TATA box upstream of the U6 gene

39 We have identified a complex between TFIIIB and the upstream promoter of silkworm tRNA Ala ge

40 A physical interaction between TFIIIB and RB is indicated by fractionation, pull-down a

41 IIB-DNA complex via the interactions between TFIIIB and hRPC39.

42 ription by altering the interactions between TFIIIB and its regulators and thus compromising its abil

43 subdomain is primarily required for binding TFIIIB and the B subdomain for binding TFIIIC2.

44 We compared TBP binding, TFIIIB-promoter complex stability (measured by heparin r

45 These interactions may facilitate both TFIIIB and RNA polymerase III recruitment to the preinit

46 tional interactions that may facilitate both TFIIIB and RNA polymerase III recruitment.

47 st direct involvement in recruitment of both TFIIIB and RNA polymerase III.

48 assays indicate that Rb interacts with both TFIIIB and TFIIIC2 and that the A subdomain is primarily

49 Brf1-TFIIIB recruits Pol III to type 1 and 2 promoters and Br

50 ng TBP and Bdp1 and referred to here as Brf1-TFIIIB and Brf2-TFIIIB, in the recruitment of Pol III.

51 with the recombinant factors SNAPc and Brf2-TFIIIB combined with purified pol III.

52 s Pol III to type 1 and 2 promoters and Brf2-TFIIIB to type 3 promoters such as the human U6 promoter

53 and referred to here as Brf1-TFIIIB and Brf2-TFIIIB, in the recruitment of Pol III.

54 Here we show that CK2 inhibits Brf2-TFIIIB by specifically phosphorylating its Bdp1 componen

55 for transcription, whereas treatment of Brf2-TFIIIB is inhibitory.

56 CK2, including the Bdp1 subunit of the Brf2-TFIIIB complex, and RNA polymerase III, with negative an

57 re-initiation complex assembly with the Brf2-TFIIIB complex.

58 icantly reduced, whereas promoter binding by TFIIIB is undiminished.

59 racteristics showed a very high occupancy by TFIIIB and pol III (pol III occupancy being generally ap

60 s with TBP, preventing promoter occupancy by TFIIIB.

61 c-Myc also stimulates pol III recruitment by TFIIIB.

62 However, hypoxic conditions cause TFIIIB dissociation from c-Myc and ERK, at the same time

63 Saccharomyces cerevisiae TFIIIB is a complex of three subunits, TBP, the TFIIB-re

64 In contrast, formation of complete TFIIIB complexes required 15 bp both upstream and downst

65 precipitate with the hBRF-containing complex TFIIIB derived from HeLa cells infected with a recombina

66 In contrast, the TBP-containing complex TFIIIB restores adenovirus VAI but not human U6 transcri

67 the pol III-specific factor Bdp1 constitute TFIIIB.

68 at the TATA-box DNA-protein complex contains TFIIIB TAF activity.

69 In contrast, TFIIIB displays a broad preference for missing nucleosid

70 In contrast, TFIIIB remained bound, suggesting that TFIIIB release is

71 Conversely, TFIIIB interacts in healthy cells with the tumour suppre

72 We further show that an SWI3-ADA2-N-CoR-TFIIIB (SANT) domain of p400 binds directly to the histo

73 In contrast, promoter opening-defective TFIIIB mutants generate significant changes of cross-lin

74 II transcription activity to these defective TFIIIB assemblies has been analyzed using U6 snRNA gene

75 de interference analysis of TFIIIC-dependent TFIIIB-DNA complex formation revealed enhanced complex f

76 Thus, pol III utilizes different TFIIIB complexes at structurally distinct promoters.

77 model in which hyperphosphorylation disrupts TFIIIB during mitosis, compromising its ability to suppo

78 BRF and propose a model in which RB disrupts TFIIIB by mimicking these two components.

79 1 (BRF1), and TFIIIB", whereas two distinct TFIIIB activities, TFIIIB-alpha and TFIIIB-beta, have be

80 lation increases expression of the essential TFIIIB subunit Brf1, an effect not seen when fibroblasts

81 ding protein (TBP)-containing general factor TFIIIB, thereby compromising its function severely.

82 The RNA polymerase III factor TFIIIB forms a stable complex with DNA and can promote m

83 gets specifically the general pol III factor TFIIIB.

84 as well as its associated initiation factor TFIIIB and is phosphorylated in a serum-sensitive manner

85 orrelated with that of the initiation factor TFIIIB and Pol III.

86 cal studies identified the initiation factor TFIIIB as a target of Maf1-dependent repression and reve

87 lymerase III transcription initiation factor TFIIIB contains the TATA-box-binding protein (TBP) and p

88 The RNA polymerase III initiation factor TFIIIB is assembled onto DNA through interactions involv

89 se (pol) III transcription initiation factor TFIIIB is bipartite; its N-terminal TFIIB-related half i

90 ant role in assembling the initiation factor TFIIIB on genes transcribed by RNA polymerase III (Pol I

91 A polymerase III (pol III) initiation factor TFIIIB.

92 via phosphorylation of the initiation factor TFIIIB.

93 DNA-bound complexes of the initiation factor TFIIIB.

94 of the RNA polymerase III initiation factor TFIIIB.

95 Brf1 and Bdp1, to form the initiation factor TFIIIB.

96 ociate with the Pol III transcription factor TFIIIB and that overexpression of p65 induces Pol III-de

97 e III (pol III) general transcription factor TFIIIB is composed of three subunits; the TATA-binding p

98 Transcription factor TFIIIB plays key roles in transcription by RNA polymeras

99 the RNA polymerase III transcription factor TFIIIB, is required for periodic integration of Ty1 into

100 ing the BRF1 subunit of transcription factor TFIIIB.

101 I TAFs, to form Pol III transcription factor TFIIIB.

102 NA pol III requires the transcription factor TFIIIB.

103 ential pol III-specific transcription factor TFIIIB.

104 lymerase III (Pol III) transcription factor (TFIIIB); TATA box binding protein (TBP) and Brf1 are the

105 at precisely position the initiation factor, TFIIIB, on the upstream promoter-less DNA.

106 f the pol III-specific transcription factors TFIIIB or TFIIIC2.

107 specific TBP-TATA contacts are important for TFIIIB-promoter interaction and for transcriptional acti

108 that no external DNA scaffold is needed for TFIIIB and RNA polymerase III binding, and that producti

109 en promote their cooperative binding to form TFIIIB-alpha.

110 rivatives were tested for competence to form TFIIIB-DNA complexes by TFIIIC-dependent and -independen

111 ce residue mutants for their ability to form TFIIIB-DNA complexes reliant on only the TFIIB-related p

112 lytic retraction in disengaging pol III from TFIIIB is discussed.

113 On tRNA genes, TFIIIB and TFIIIC form stable complexes with the same di

114 Human TFIIIB is not as well defined and human pol III promoter

115 Human TFIIIB-beta is required for transcription of genes with

116 vitro interactions with the homologous human TFIIIB and RNA polymerase III components, as well as add

117 g with TBP and TFIIIB150, reconstitute human TFIIIB-alpha activity.

118 munoprecipitation using subunits of Pol III, TFIIIB and TFIIIC.

119 A high degree of homology occurs in pol III, TFIIIB, TFIIIA and the three initiation-related subunits

120 NA polymerase III transcription factor IIIB (TFIIIB) complex has been identified using antibodies dir

121 l III) transcription initiation factor IIIB (TFIIIB) in the absence of TFIIIC or other assembly facto

122 pol III-specific transcription factor IIIB (TFIIIB) is bound and regulated by the proto-oncogene pro

123 Transcription factor IIIB (TFIIIB) is composed of the TATA box binding protein (TBP

124 omyces cerevisiae transcription factor IIIB (TFIIIB) is composed of three subunits: the TATA-binding

125 Transcription factor IIIB (TFIIIB) is directly involved in transcription initiation

126 romoters requires transcription factor IIIB (TFIIIB), an activity that binds to RNA polymerase III pr

127 Transcription factor IIIB (TFIIIB), consisting of the TATA-binding protein (TBP), T

128 Transcription factor IIIB (TFIIIB), the central transcription factor of Saccharomyc

129 d TBP to form the transcription factor IIIB (TFIIIB), which recruits Pol III to target genes.

130 In humans, transcription factor IIIB (TFIIIB)-alpha governs basal transcription from small nuc

131 the polymerase on transcription factor IIIB (TFIIIB)-DNA complexes that are stable through multiple r

132 se III transcription initiation factor IIIB (TFIIIB).

133 equires transcription factors IIIB and IIIC (TFIIIB and TFIIIC).

134 romoter and characterize the role of Bdp1 in TFIIIB assembly and pre-initiation complex formation.

135 es known to contact either TFIIA or Brf1 (in TFIIIB).

136 ependent repression and revealed a defect in TFIIIB-DNA complex assembly under repressing conditions.

137 the DNA binding domain of TBP is exposed in TFIIIB.

138 Further analysis shows that these inactive TFIIIB-DNA complexes bind pol III and position it approp

139 of several key anabolic elements, including TFIIIB, TFIID, RNA polymerase II, poly(A) polymerase, an

140 ription of the VA1 and tRNA genes, including TFIIIB, TFIIIC, and RNA Pol III, can be coimmunopurified

141 The individual TFIIIB subunits are nuclear by immunofluorescence and ar

142 mutations in the dT-dA tract do not inhibit TFIIIB binding or TFIIIC-independent transcription of SN

143 Blocking the ERK signalling cascade inhibits TFIIIB binding to pol III and to transcription factor TF

144 2 and 284 to 596 were found to assemble into TFIIIB-DNA and TFIIIC-TFIIIB-DNA complexes that were ver

145 ctural insight on how Bdp1 is assembled into TFIIIB complexes, reveals structural and functional simi

146 led a defect in the recruitment of Brf1 into TFIIIB-TFIIIC-DNA complexes and diminished the direct in

147 binding affinity for Bdp1 incorporation into TFIIIB-TFIIIC-DNA complexes and inhibited binary interac

148 the idea of a concerted mechanism involving TFIIIB and RNA polymerase III subunits for the closed to

149 ymerase III general transcription machinery, TFIIIB.

150 rence for AT-rich sequences, TBP can mediate TFIIIB assembly at diverse DNA sequences, including stre

151 for required DNA flexure in TFIIIC-mediated TFIIIB-DNA complex formation was pursued in a TFIIIC-ind

152 rtly open, but also it is detected in mutant TFIIIB-pol III-DNA complexes that are unable to open the

153 CoREST contains two SANT (SW13/ADA2/NCoR/TFIIIB B) domains, a structural feature of the nuclear r

154 Surprisingly, TFIIIC, but not TFIIIB, interacts with some centromeric nucleosomes, sug

155 cupancy being generally approximately 70% of TFIIIB occupancy) and a TFIIIC occupancy that ranged bet

156 ted, underscoring the unexplained absence of TFIIIB at those sites.

157 es in favor of an indirect mode of action of TFIIIB in promoter opening.

158 olymerase (Pol) III requires the activity of TFIIIB, a complex formed by Brf1 (or Brf2), TBP (TATA-bi

159 r DNA, was used to probe the architecture of TFIIIB subunits bound to DNA.

160 e reveals the core interface for assembly of TFIIIB and demonstrates how the loosely packed Brf1 doma

161 However, assembly of TFIIIB into a pre-initiation complex confers substantial

162 ing the steps in TFIIIC-directed assembly of TFIIIB, we conducted yeast two-hybrid screens of Brf1 pe

163 competent to participate in the assembly of TFIIIB-DNA complexes and in TFIIIC-independent transcrip

164 or Bdp1, in the TFIIIC-dependent assembly of TFIIIB.

165 We show that the association of TFIIIB with tRNA genes proceeds through an initial step

166 C is necessary for insulation but binding of TFIIIB along with TFIIIC likely improves the probability

167 Direct binding of TFIIIB to RPC6 is believed to recruit pol III to its gen

168 tt109 were required for efficient binding of TFIIIB to the tRNA insulator, and the bound transcriptio

169 h DNA-bound TBP and with the B" component of TFIIIB to be retained.

170 of U6 snRNA or Brf1, a limiting component of TFIIIB, and an activating mutation (PCF1-1) in TFIIIC we

171 ound TFIIIC and with the other components of TFIIIB and for participation in transcription.

172 procity between the Brf and B" components of TFIIIB.

173 nscription through the coordinate control of TFIIIB subunit expression and elucidate opposing functio

174 dehyde cross-linked in vitro counterparts of TFIIIB-, TFIIIC-, and pol III-DNA complexes, measuring t

175 IIC occupancy and instead to a dependence of TFIIIB-DNA and TFIIIC-DNA complex maintenance in vivo on

176 sformed cells; the resultant derepression of TFIIIB may provide an almost universal route to deregula

177 Hydroxyl radical footprinting of TFIIIB complexes and modeling the results to the TBP-DNA

178 In yeast, a single form of TFIIIB is required for transcription of all RNA polymera

179 It is proposed that the normal function of TFIIIB combines pol III recruitment with an active role

180 The principal function of TFIIIB is to recruit pol III to its cognate gene templat

181 to phosphorylation-mediated inactivation of TFIIIB, an essential complex comprising the TATA-binding

182 actions were implicated in the inhibition of TFIIIB.DNA complex assembly and polymerase recruitment b

183 this complex to compare the interactions of TFIIIB with two kinds of tRNA Ala genes whose different

184 c photochemical protein-DNA cross-linking of TFIIIB-pol III-U6 RNA gene promoter complexes.

185 genome-wide high-resolution footprint map of TFIIIB-TFIIIC complexes in Saccharomyces cerevisiae, obt

186 ented cellular levels of protein and mRNA of TFIIIB subunits, Brf1 and TATA box-binding protein (TBP)

187 esence of TFIIIC specifies an orientation of TFIIIB for transcriptional initiation and directs integr

188 ent of tRNA genes specifies the placement of TFIIIB on upstream-lying DNA.

189 eam do not lead to preferential placement of TFIIIB.

190 ion occurs due to heterogeneous placement of TFIIIB.

191 In the presence of TFIIIB and TFIIIC, the pattern of integration extended d

192 ut detectable frequencies in the presence of TFIIIB subunits Brf1 and TATA-binding protein.

193 tion was first provided by the properties of TFIIIB-RNA polymerase III-promoter complexes assembled w

194 ombinant Maf1 can inhibit the recruitment of TFIIIB and RNA pol III to immobilized templates.

195 e 5S RNA gene) and subsequent recruitment of TFIIIB and RNA polymerase III.

196 ated by alcohol through the co-regulation of TFIIIB components and define a central role for c-Jun in

197 em offers an opportunity to test the role of TFIIIB independent of a requirement of TFIIIC.

198 order to investigate the respective roles of TFIIIB and TFIIIC, we have developed an in vitro integra

199 chemical evidence that the high stability of TFIIIB-DNA complexes results from kinetic trapping of th

200 (TFIIIC131) and the TFIIB-related subunit of TFIIIB (TFIIIB70/Brf1).

201 achment site of Brf1 for the Bdp1 subunit of TFIIIB has been mapped by a combination of structure-inf

202 The Brf1 subunit of TFIIIB plays an important role in recruiting the TATA-bi

203 own of MAF1 with Pol III or BRF1 (subunit of TFIIIB) diminished the activation and looping effect, wh

204 subunits of TFIIIC, the hTFIIIB90 subunit of TFIIIB, and the human RPC39 (hRPC39) and hRPC62 subunits

205 Our results suggest that a subunit of TFIIIB, possibly TBP, makes an essential minor groove DN

206 p -23 was shown to require the B" subunit of TFIIIB.

207 " and TFIIB-related factor (BRF) subunits of TFIIIB are positioned on opposite sides of the TBP-DNA c

208 e affinities that hold the three subunits of TFIIIB together.

209 e assembly factor TFIIIC and two subunits of TFIIIB, Brf1 and Bdp1.

210 All three subunits of TFIIIB, TBP, Brf (the TFIIB-related subunit) and B", are

211 that the recombinant Brf and TBP subunits of TFIIIB, which interact over the SNR6 TATA box, direct in

212 ription machinery and show that two types of TFIIIB activities, with specificities for different clas

213 plex, confers extremely tight DNA binding on TFIIIB.

214 thout destabilizing core pol III subunits or TFIIIB.

215 site in the absence of detectable TFIIIC or TFIIIB subunit B".

216 s the recruitment of pol III to preassembled TFIIIB.DNA complexes.

217 The principal TFIIIB-assembly function of Brf was found to be contribu

218 Repression conditions also promote TFIIIB-TFIIIC interactions in crosslinked chromatin.

219 ied region where missing nucleosides promote TFIIIB-DNA complex formation, also result in enhanced an

220 site may provide a DNA flexure that promotes TFIIIB-DNA complex formation.

221 Addition of either purified TFIIIB or purified TFIIIC2 partially relieves Rb-mediate

222 III transcription apparatus into recombinant TFIIIB, highly purified TFIIIC, and pol III is accompani

223 Using recombinant TFIIIB subunits, we found that TFIIIB90 interacts weakly

224 polypeptides were sufficient to reconstitute TFIIIB transcriptional activity and DNA binding activity

225 c A block and downstream B block can recruit TFIIIB via protein-protein interactions.

226 ly recognizes promoter elements and recruits TFIIIB and RNA polymerase III.

227 on initiation by RNA polymerase III requires TFIIIB, a complex formed by Brf1/Brf2, TBP and Bdp1.

228 rom the gene-internal tRNA promoter requires TFIIIB composed of Bdp1, TBP, and Brf1.

229 ene-external U6 snRNA transcription requires TFIIIB consisting of Bdp1, TBP, and Brf2.

230 n Saccharomyces cerevisiae Brf and B" retain TFIIIB transcription factor activity with supercoiled DN

231 Selected TFIIIB-TFIIIC-DNA complexes assembled with truncated B"

232 Since TFIIIB is an essential determinant of the biosynthetic c

233 Yeast contain a single TFIIIB activity that is comprised of the TATA-binding pr

234 mation, also result in enhanced and specific TFIIIB assembly; 4-nt loops further downstream do not le

235 TBP and Brf2, a subunit of a snRNA-specific TFIIIB complex.

236 ription in vitro, and forms extremely stable TFIIIB-DNA complexes that are indistinguishable from wil

237 found to assemble into TFIIIB-DNA and TFIIIC-TFIIIB-DNA complexes that were very stable, transcriptio

238 We conclude that TFIIIB induces an additional DNA deformation between the

239 in immunoprecipitation assays, we found that TFIIIB occupancy on tRNA genes markedly decreases follow

240 f this suppression phenomenon indicates that TFIIIB participates in two steps of promoter opening by

241 tion in vitro, and it has been proposed that TFIIIB suffices for pol III recruitment in vivo.

242 We show that TFIIIB activity is elevated in primary fibroblasts from

243 tion in RB-treated extracts, suggesting that TFIIIB is important for RB regulation of tRNA-like genes

244 rast, TFIIIB remained bound, suggesting that TFIIIB release is not a critical aspect of the onset of

245 The TFIIIB subunits Brf1 and Brf2 are structurally similar t

246 In addition, the TFIIIB complex formed on tRNA SG Ala genes differ qualit

247 n examined for the abilities to assemble the TFIIIB-DNA complex and recruit RNA polymerase III to acc

248 results extend the similarities between the TFIIIB domains required for transcription and Ty3 integr

249 t disrupt the direct interaction between the TFIIIB subunits TBP and Brf1, but prevents the associati

250 However, seven loci that bind the TFIIIB loader, TFIIIC, were not targeted, underscoring t

251 These results further delimit the TFIIIB domains targeted by the Ty3 element and show that

252 B", the last component to enter the TFIIIB-DNA complex, confers extremely tight DNA binding

253 re the role that release of pol III from the TFIIIB-DNA complex plays in limiting the overall rate of

254 that is directly associated with TBP in the TFIIIB complex, dTAFIII105, and an amino acid residue wi

255 e further probed the TBP interactions in the TFIIIB*DNA complex to elucidate the mechanism for the di

256 ing, and the surface topography of B" in the TFIIIB-DNA complex was also analyzed by hydroxyl radical

257 Thus, TBP in the TFIIIB-SUP4 gene promoter region is bound in the same di

258 imary interface for assembling Bdp1 into the TFIIIB complex.

259 ain is also required, as are subunits of the TFIIIB and RSC complexes that bind the gene.

260 In Saccharomyces cerevisiae, assembly of the TFIIIB complex by promoter-bound TFIIIC is thought to be

261 on opposite sides of the TBP-DNA core of the TFIIIB complex, as indicated by correlation of cross-lin

262 ility of ethanol to induce expression of the TFIIIB components, Brf1, and the TATA-binding protein (T

263 print over box A and reduces the size of the TFIIIB footprint on upstream DNA sequence.

264 This is accomplished by the ability of the TFIIIB subunit, TFIIB-related factor (Brf1), to make sta

265 Although human homologs for two of the TFIIIB subunits, the TATA box-binding protein TBP and th

266 mal segment of B" to the upstream end of the TFIIIB-DNA complex and amino acids 299-315 to the princi

267 would help explain the long half-life of the TFIIIB-DNA complex and its resistance to polyanions and

268 The high stability of the TFIIIB-DNA complex is conferred by TFIIIB90 binding to T

269 The picture of the TFIIIB-DNA complex that emerges is that B" serves as its

270 and II of B" are buried upon assembly of the TFIIIB-DNA complex, as determined by protein footprintin

271 n extended interface along the length of the TFIIIB-DNA complex.

272 mapped by photochemical cross-linking of the TFIIIB-DNA complex.

273 f and how they fit into the structure of the TFIIIB-DNA complex.

274 hrough the inactivation of mTOR, targets the TFIIIB complex, disrupting the association between TATA-

275 Global analysis reveals that the TFIIIB-TFIIIC transcription complex exhibits remarkable

276 ubcomplex directs RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB a

277 ase III (pol III) stably associates with the TFIIIB-DNA complex even in the absence of localized DNA

278 Although it is within the TFIIIB footprint, point mutations in the dT-dA tract do

279 The orientation of the B" subunit within the TFIIIB-DNA complex has been analyzed at two promoters by

280 ility to limit pol III transcription through TFIIIB may contribute to their growth-suppression capaci

281 We demonstrate here that c-Myc binds to TFIIIB, a pol III-specific general transcription factor,

282 lity requires the presence of Nhp6a prior to TFIIIB-DNA complex formation.

283 ion that a TBP-containing complex related to TFIIIB is required for the transcription of class III sn

284 In turn, TFIIIB recruits pol III to the promoter and specifies tr

285 inactivation of transcription with wild-type TFIIIB generated by certain transcribed (template) stran

286 es that are indistinguishable from wild-type TFIIIB-DNA complexes by chemical nuclease footprinting.

287 the presence of MnCl(2), strand transfer was TFIIIB-independent and targeted sequences resembling the

288 0), and a BRF1 homologue (TFIIIB90), whereas TFIIIB-alpha is required for transcription of genes with

289 gh the minor groove of DNA, we asked whether TFIIIB interacts with DNA in the minor groove.

290 core enzyme is defective in associating with TFIIIB and target genes in vivo.

291 nits RPC3, RPC6 and RPC7, and interacts with TFIIIB, a factor that is necessary and sufficient to sup

292 I-mediated transcription by interfering with TFIIIB and Pol III.

293 re repressed through interaction of p53 with TFIIIB, a TATA-binding protein (TBP)-containing factor.

294 The ETC sites, where TFIIIC binds without TFIIIB, exhibit different bootprints, suggesting that TF

295 the TATA-box DNA binding activity of Xenopus TFIIIB, using transcription inhibition assays and a gel

296 NA binding activity, suggesting that Xenopus TFIIIB is composed of TBP along with these polypeptides.

297 a human homologue of the B" subunit of yeast TFIIIB generally required for transcription of RNA polym

298 The interaction of yeast TFIIIB with the region upstream of the SUP4 tRNATyr gene

299 promoter (A box) and with a subunit of yeast TFIIIB.

300 FU, one of two human homologues of the yeast TFIIIB subunit BRF specifically required for transcripti