ライフサイエンスコーパス: TFP

1 TFP also act as external sensor to regulate EPS producti

2 TFP are filamentous surface appendages primarily compose

3 TFP are known to bind to secreted exopolysaccharides (EP

4 TFP deficiency causes a Reye-like syndrome, cardiomyopat

5 TFP+AL together were completely ineffective in preservin

6 TFP-deficient M. catarrhalis bacteria exhibit diminished

7 TFP-dependent gliding motility has been seen in many Gra

8 injury in the NHBD than MPS alone, AL, or AL+TFP.

9 that surface sensing may be widespread among TFP-expressing bacteria.

10 including T3SS, exotoxin A, protease IV, and TFP.

11 t a surface with low levels of piliation and TFP activity, which both progressively increase as the c

12 the proteolytic cleavage site between PR and TFP revealed the presence of an additional site and the

13 ence of a p1 peptide separating protease and TFP.

14 elationship between cAMP, the Chp system and TFP regulation.

15 To assess TFP diversity in Gram-positive bacteria and facilitate p

16 inhibition of S100A4 function occurs only at TFP concentrations that promote S100A4 oligomerization.

17 s of expansion medium supplementation (bFGF, TFP, FBS) and self-assembled construct seeding density (

18 (TFP) on solid surfaces, which requires both TFP and exopolysaccharides (EPS).

19 rs and that biofilm formation is enhanced by TFP expression.

20 tter, levels of inhibition by 125-C9 than by TFP and W-13.

21 volved in the biosynthesis of M. catarrhalis TFP and determined that the TFP expressed by this organi

22 Combined, TFP and Low density interact to further enhance construc

23 , but are instead consistent with a constant TFP-generated force interacting with EPS, which function

24 On surface contact, TFP retraction activates the Chp chemosensory system pho

25 pilins, including 214 in operons containing TFP biosynthetic genes (TBG+ operons).

26 endent transcriptional regulator Vfr control TFP biogenesis gene expression, we explored the relation

27 We discovered that the Chp system controls TFP production through modulation of cAMP while control

28 In cells with Ca(2+) stores depleted, TFP further increased CSQ1 monomerization and CSQ1-STIM1

29 that loading of either monomeric or dimeric TFP cargo occurs with unprecedented high fidelity and ex

30 ther bacterial species simultaneously encode TFP, the Chp system, FimL, FimV and adenylate cyclase ho

31 Genes encoding TFP are also found in the genomes of all nine Clostridiu

32 Extended TFP that are composed of thousands of copies of PilA ret

33 We delineated the molecular basis for TFP deficiency in two patients with a unique phenotype c

34 Here, we build a simple model for TFP-driven surface motility without complications from v

35 While PilG phosphorylation is required for TFP function and mechanochemical signaling, it is not re

36 rotease by the viral TFP suggests a role for TFP in regulating protease function during HIV-1 replica

37 ever, are unable to assemble into functional TFP despite their ability to localize to the membrane.

38 ower in a pilT mutant that lacked functional TFP.

39 However, TFP were not associated with the bacteria in the biofilm

40 approved drug trifluoperazine hydrochloride (TFP), which has been shown to inhibit FOXO1 nuclear expo

41 ation of the fluorescence of the immobilized TFP and the fluorogenic group provided a direct tool to

42 ts a novel genotype-phenotype correlation in TFP deficiency; that is, mutations in exon 9 of the alph

43 ry light chain (RLC) phosphorylation only in TFP-treated, but not in untreated, permeabilized smooth

44 ible to understand the interaction of intact TFP with HIV-1 protease under conditions of crystal grow

45 This predicted physical mechanism involving TFP was confirmed in vitro using pairwise mixtures of st

46 of wild-type M. catarrhalis and an isogenic TFP mutant to colonize the nasopharynx of the chinchilla

47 bacterial species (>13,000 proteins) lacking TFP biosynthetic genes.

48 henyl)iodonium hexafluorophosphate (0.05 M), TFP (0.1 M), and PAIBN (0.02 M) was illuminated with a 6

49 MPS+TFP more effectively protected renal function against re

50 ned to a perfusate group (G): G1=MPS, G2=MPS+TFP, G3=MPS+AL, and G4=MPS+TFP+AL.

51 ): G1=MPS, G2=MPS+TFP, G3=MPS+AL, and G4=MPS+TFP+AL.

52 hat the former is due to pulling by multiple TFP, whereas the latter is due to release by single TFP.

53 er of species were found to contain multiple TFP operons (pil, com and/or tad).

54 ey lacked EPS; however, they retained normal TFP and LPS.

55 Transframe octapeptide (TFP) Phe-Leu-Arg-Glu-Asp-Leu-Ala-Phe, the N terminus of

56 Assays examining the ability of TFP to block S100A4-mediated disassembly of myosin-IIA f

57 scaffold enabling spatial colocalization of TFP and Chp system components to coordinate signaling le

58 The LC17-induced contractions of TFP-treated muscles were abolished by prior addition of

59 l study by investigating the contribution of TFP to the early stages of M. catarrhalis colonization.

60 through modulation of cAMP while control of TFP-dependent twitching motility is cAMP-independent.

61 These results suggest that the expression of TFP contributes to mucosal airway colonization.

62 In conclusion, the interaction of TFP and Low density seeding enhances construct material

63 olished motility and surface localization of TFP.

64 Moreover, a surprisingly small number of TFP are needed to recapitulate movement signatures assoc

65 ular self-sorting and selective packaging of TFP cargo into bacterial encapsulins during in vivo asse

66 e we discern a passive intercellular role of TFP during flagellar-mediated swarming of P. aeruginosa

67 The potential role of TFP in colonization by M. catarrhalis was further invest

68 oldest Eubacterial class and the ubiquity of TFP in this class suggests that a Clostridia-like ancest

69 including the T2SS, the T3SS, flagellum- or TFP-dependent motility, virulence in a mouse model of ac

70 ect on virus replication, mutation of the p1-TFP cleavage site led to noninfectious virus and the los

71 These findings implicate C. perfringens TFP in the ability of C. perfringens to adhere to and mo

72 as in tris(2,2,2-trifluoroethyl) phosphite (TFP), allows polymerization to proceed with a minimal am

73 t two cell surface structures: type IV pili (TFP) and extracellular polysaccharides (EPS).

74 pendages known as flagella and type IV pili (TFP) are known to confer such motility.

75 s for surface exploration uses type-IV pili (TFP) as linear actuators to enable directional crawling.

76 ously shown that expression of type IV pili (TFP) contributes to mucosal colonization.

77 Type IV pili (TFP) function as mechanosensors to trigger acute virulen

78 In Gram-negative bacteria, type IV pili (TFP) have long been known to play important roles in suc

79 he extension and retraction of type IV pili (TFP) on solid surfaces, which requires both TFP and exop

80 ) and their associated toxins, type IV pili (TFP), and flagella.

81 e III secretion system (T3SS), type IV pili (TFP), and multiple secreted toxins and degradative enzym

82 lity has been shown to require type IV pili (TFP), exopolysaccharide (EPS; a component of fibrils) an

83 Type IV pili (TFP), filamentous surface appendages primarily comprised

84 ility mechanism is mediated by type IV pili (TFP), linear actuator appendages that propel the bacteri

85 oss surfaces by using multiple Type IV Pili (TFP), motorized appendages capable of force generation v

86 , attachment and retraction of type IV pili (TFP), which pull the bacterium towards the site of attac

87 s lacks flagella but possesses type IV pili (TFP).

88 the production and function of type IV pili (TFP).

89 some gram-negative bacteria, type IV pilus (TFP)-dependent gliding motility was necessary for effici

90 ce of agar plates by a unique type IV pilus (TFP)-mediated social motility that had not been previous

91 ts that a Clostridia-like ancestor possessed TFP, which evolved into the forms seen in many Gram-nega

92 d frameshift site, and hence did not produce TFP, was noninfectious.

93 Here we show that C. perfringens produces TFP and moves with an unusual form of gliding motility i

94 entified gene products involved in producing TFP in each strain.

95 bilization of a visible fluorescent protein (TFP) modified to display a single cysteine residue was d

96 Thiocyanate-forming protein (TFP) from field-penny cress, Thlaspi arvense (Brassicace

97 ely detected as an 8-kDa transframe protein (TFP) in virions.

98 Human mitochondrial trifunctional protein (TFP) is a heterooctamer of four alpha- and four beta-sub

99 Using teal fluorescent proteins (TFP) engineered with a specific native C-terminal dockin

100 Remarkably, TFP binding results in the assembly of five Ca(2+)-S100A

101 rming of P. aeruginosa that does not require TFP extension or retraction.

102 esults in the assembly of five Ca(2+)-S100A4/TFP dimers into a tightly packed pentameric ring.

103 rative formation of a similarly sized S100A4/TFP oligomer in solution.

104 Results show TFP (1 ng/mL TGF-beta1, 5 ng/mL bFGF, 10 ng/mL PDGF) sup

105 ereas the latter is due to release by single TFP.

106 sh a regulatory link between the Chp system, TFP and other cAMP-dependent virulence systems.

107 A tetrafluorphenyl (TFP) ester of trimethyl psoralen (trioxalen) was synthes

108 ld be distributed all over the cell and that TFP-TFP interactions between cells should be a dominant

109 h the crystal structure and demonstrate that TFP binds to the target binding cleft of S100A4 in solut

110 he unanticipated structural requirement that TFP motors need to have a minimal amount of effective an

111 We show that TFP-mediated crawling in Pseudomonas aeruginosa consiste

112 Our simulations showed that TFP of swarming P. aeruginosa should be distributed all

113 slow swarm expansion, we show in vitro that TFP help alter collective motion to avoid toxic compound

114 The TFP and AL combination should not be used together with

115 ubstitution of the acidic amino acids in the TFP by neutral amino acids and d or retro-d configuratio

116 most effective solution for HBD kidneys, the TFP additive (perfusate G2) more effectively reversed th

117 formation including a catalytic role of the TFP involved.

118 directly links the integral component of the TFP structural complex FimV, a peptidoglycan binding pro

119 f M. catarrhalis TFP and determined that the TFP expressed by this organism are highly conserved and

120 e necessary for S-motility: We show that the TFP of leading "locomotive" cells initiate the collectiv

121 cts between S100A4 dimers occurs through the TFP moieties.

122 is unclear how M. xanthus manages to use the TFP-EPS technology common to many bacteria to achieve it

123 Thus, TFP physically affect P. aeruginosa swarming by actively

124 al structure of human Ca(2+)-S100A4 bound to TFP.

125 ll-studied CaM antagonists: trifluoperazine (TFP) and W-13.

126 molecule inhibitors such as trifluoperazine (TFP).

127 ique biosensor-based assay, trifluoperazine (TFP) was identified as an inhibitor that disrupts the S1

128 if allopurinol (AL) and/or trifluoperazine (TFP) added to the Belzer machine preservation solution (

129 depleted Ca(2+) stores, or trifluoperazine (TFP), a blocker of CSQ folding and aggregation, enhanced

130 smooth muscle treated with trifluoperazine (TFP).

131 mylbenzene (TFB) or triformylphloroglucinol (TFP).

132 Animals coexpressing the TRIM5(TFP) and TRIM5(CypA) alleles took significantly longer t

133 Mutant viruses with a C-terminally truncated TFP (19, 32, or 50 residues) had essentially a wild-type

134 Two TFP molecules bind within the hydrophobic target binding

135 al bacteria attached end to end and that two TFP-associated proteins, PilT and PilC, are needed for t

136 nt signatures associated with twitching: Two TFP can already produce movements reminiscent of recentl

137 nhibition of the HIV-1 protease by the viral TFP suggests a role for TFP in regulating protease funct

138 crease in density (</=-0.03 g/cm(3)) whereas TFP induced an increase in both thickness (</=0.05 nm) a

139 While TFP slow swarm expansion, we show in vitro that TFP help

140 ith CHO (k = 2 x 10(6) M(-1) s(-1)) and with TFP (k = 2 x 10(8) M(-1) s(-1)).

141 pecies, regardless of their association with TFP biosynthetic operons.

142 e cells without TFP separate from cells with TFP.

143 y entropy and appears to be competitive with TFP and W-13, which is consistent with occupation of sim

144 That is, with TFP, Low density increases type II collagen content by o

145 To examine the interaction of S100A4 with TFP, we determined the 2.3 A crystal structure of human

146 1, a lysosome marker, whereas treatment with TFP, an inducer of autophagy, resulted in decreased Col-

147 airwise mixtures of strains with and without TFP where cells without TFP separate from cells with TFP

148 ins with and without TFP where cells without TFP separate from cells with TFP.

149 um containing 1% methylcellulose, M. xanthus TFP-driven motility was induced in isolated cells and in