ライフサイエンスコーパス: TIRF

1 TIRF images were constructed from several layers within

2 TIRF imaging indicates that the granules can be triggere

3 TIRF microscopic observations of functional ElmoA-GFP re

4 TIRF microscopy and biophysical modeling of fluorescence

5 TIRF microscopy can be used in conjunction with CFP/YFP

6 TIRF was used to monitor biospecific interactions, while

7 TIRF-PBM provides a novel and extendible platform for mu

8 TIRF/FRET experiments revealed cotransfection of wild-ty

9 emonstrate, using quantitative live-cell 4D, TIRF, and FRET imaging, that endocytosis and trafficking

10 hly sensitive fluorescence immunoassay for a TIRF (total internal reflection)-based point-of-care tes

11 By attaching a rolling-circle substrate to a TIRF microscope-mounted flow chamber, we are able to mon

12 monstrate in vitro using assembly assays and TIRF microscopy, and in primary neurons using live-cell

13 umin was injected in the channel chamber and TIRF was used to determine the time to reach the assay s

14 resulting dissociation of Arp2/3 complex and TIRF microscopy to visualize filament severing and the l

15 , as judged by epifluorescent, confocal, and TIRF microscopy, but fluoresces brightly within the Ser-

16 Similarly, electrophysiological data and TIRF microscopy show that NEDD4 unrestrained mutant cons

17 Using FLIM and TIRF imaging, we find that changes in MCAK conformation

18 e effective viscosity of the bulk lipid, and TIRF microscopy indicates that it clusters in segregated

19 aneous membrane capacitance measurements and TIRF imaging.

20 malian cells with any commercially available TIRF microscope.

21 Commercially available TIRF systems are either objective based that employ expe

22 l, inexpensive, LED powered, waveguide based TIRF system that could be used as an add-on module to an

23 lection fluorescence, fiber-optic biosensor (TIRF-FOB) for protein detection, which integrates a lipo

24 ingle-vesicle fusion events in PC12 cells by TIRF micro-scopy.

25 distinct fluorescent signals as detected by TIRF microscopy.

26 ion factors TBP, TFIIA and IIB determined by TIRF-PBM are similar to those determined by traditional

27 Single-vesicle optical (by TIRF microscopy) and biophysical measurements of ATP rel

28 When tracked by TIRF and spinning-disk microscopy, cells expressing phos

29 ) trafficking pathways as shown by live cell TIRF and structured illumination microscopy (SIM).

30 amin2-EGFP instead of dynamin2 and live-cell TIRF imaging with single-molecule EGFP sensitivity and h

31 We used live-cell TIRF imaging with single-molecule EGFP sensitivity and h

32 M and dSTORM super-resolution, and live-cell TIRF microscopy to characterize the structural organizat

33 Utilizing multi-color TIRF microscopy of in vitro reconstituted F-actin networ

34 However, two-color TIRF microscopy using fluorescent proteins fused to clat

35 Using a microfluidics-assisted multi-colour TIRF microscopy assay with close-to-nm and sub-second pr

36 By combining TIRF microscopy and a stochastic model of exocytosis, we

37 and allows switching between epifluorescence/TIRF/bright field modes without adjustments or objective

38 ese nanoparticles using ITC, DLS, FRET, FCS, TIRF, and TEM.

39 on cryoEM reconstruction and single filament TIRF microscopy we identify two dynamic and structural s

40 ing total internal reflectance fluorescence (TIRF) microscopy, we found that beta-catenin is required

41 of total internal reflectance fluorescence (TIRF) spectroscopy, swellable hydrogel double-stranded D

42 sing total internal reflection fluorescence (TIRF) and confocal microscopy, we studied the mechanisms

43 h as total internal reflection fluorescence (TIRF) and Forster resonance energy transfer (FRET) has p

44 ular total internal reflection fluorescence (TIRF) configuration.

45 of a total internal reflection fluorescence (TIRF) flow cell.

46 T in total-internal reflection fluorescence (TIRF) Forster resonance energy transfer (TIRF-FRET) micr

47 cing total internal reflection fluorescence (TIRF) images that are evenly lit.

48 Total-internal reflection fluorescence (TIRF) imaging is capable of determining membrane-binding

49 ion, total internal reflection fluorescence (TIRF) imaging was used to visualize the migration of flu

50 with total internal reflection fluorescence (TIRF) imaging.

51 ngle total internal reflection fluorescence (TIRF) microscope.

52 with total internal reflection fluorescence (TIRF) microscopy allowed us to image GFP-tagged SMSr pro

53 cule total internal reflection fluorescence (TIRF) microscopy and allows the probing of single macrom

54 Total internal reflection fluorescence (TIRF) microscopy and its variants are key technologies f

55 sing total internal reflection fluorescence (TIRF) microscopy and observed intramolecular condensatio

56 sing total internal reflection fluorescence (TIRF) microscopy and patch-clamp recording from single J

57 ined total internal reflection fluorescence (TIRF) microscopy and patch-clamp recording to localize S

58 cule total internal reflection fluorescence (TIRF) microscopy constitutes an umbrella of powerful too

59 itro total internal reflection fluorescence (TIRF) microscopy demonstrated that Tpm1 strongly enhance

60 ore, total internal reflection fluorescence (TIRF) microscopy imaging of single actin filaments confi

61 u by total-internal-reflection fluorescence (TIRF) microscopy imaging.

62 and total internal reflection fluorescence (TIRF) microscopy in combination with fluorescence recove

63 and total internal reflection fluorescence (TIRF) microscopy in vitro, and the mechanism mimics the

64 Total internal reflection fluorescence (TIRF) microscopy is a rapidly expanding optical techniqu

65 apse total internal reflection fluorescence (TIRF) microscopy is used to directly measure the kinetic

66 tion total internal reflection fluorescence (TIRF) microscopy of live cells, we followed the movement

67 and total internal reflection fluorescence (TIRF) microscopy of living mammalian cells and correlati

68 and total internal reflection fluorescence (TIRF) microscopy revealed that HSV-1 was released at spe

69 Total internal reflection fluorescence (TIRF) microscopy reveals highly mobile structures contai

70 olor total internal reflection fluorescence (TIRF) microscopy reveals that a low number of INF2 molec

71 apse total internal reflection fluorescence (TIRF) microscopy showed that signaling via the T cell an

72 tive total internal reflection fluorescence (TIRF) microscopy system to directly visualize the moveme

73 sing total internal reflection fluorescence (TIRF) microscopy to image Ca(2+) influx in Xenopus laevi

74 and Total Internal Reflection Fluorescence (TIRF) microscopy to measure lateral diffusion coefficien

75 use total internal reflection fluorescence (TIRF) microscopy to probe individual QDs immobilized on

76 Total internal reflection fluorescence (TIRF) microscopy was used to quantify the growth of sing

77 nce, total internal reflection fluorescence (TIRF) microscopy, and live-cell photoactivation localiza

78 nder total internal reflection fluorescence (TIRF) microscopy, and patch-clamp analysis.

79 nder total internal reflection fluorescence (TIRF) microscopy, in which excitation light only penetra

80 By total internal reflection fluorescence (TIRF) microscopy, Scrib and integrin alpha5 colocalize a

81 olor total internal reflection fluorescence (TIRF) microscopy, single particle tracking and motility

82 and total internal reflection fluorescence (TIRF) microscopy, we demonstrate that glucose stimulates

83 d by total internal reflection fluorescence (TIRF) microscopy, we observed a positive FRET signal.

84 sing total internal reflection fluorescence (TIRF) microscopy, we studied the mechanisms of surface m

85 nder total internal reflection fluorescence (TIRF) microscopy.

86 dard total internal reflection fluorescence (TIRF) microscopy.

87 sing total internal reflection fluorescence (TIRF) microscopy.

88 cule total internal reflection fluorescence (TIRF) microscopy.

89 with Total Internal Reflection Fluorescence (TIRF) microscopy.

90 sing total internal reflection fluorescence (TIRF) microscopy.

91 llel total internal reflection fluorescence (TIRF) microscopy.

92 sing total internal reflection fluorescence (TIRF) microscopy.

93 sing total internal reflection fluorescence (TIRF) spectroscopy.

94 sing total internal reflection fluorescence (TIRF) with fluorescence imaging with 1-nm accuracy (FION

95 the total internal reflection fluorescence (TIRF) zone beneath the plasma membrane.

96 AP), total internal reflection fluorescence (TIRF), deconvolution, and siRNA knockdown, we propose th

97 and total internal reflection fluorescence (TIRF)-based assay to show that ensembles of kinesin-5, a

98 sing total-internal reflection fluorescence (TIRF).

99 Total-Internal-Reflection-Fluorescence (TIRF) microscopy experiments and data from a laser tweez

100 lamp, Total Internal Reflection Fluorescent (TIRF) microscopy, and fluorescence recovery after photob

101 merit of a high signal/background ratio from TIRF microscopy.

102 at gives rise to nonevanescent components in TIRF.

103 r pathological mechanism in AD and introduce TIRF imaging for massively parallel single-channel studi

104 Using time-lapse TIRF microscopy, we observed and quantified the severing

105 epth) toward the critical angle (the largest TIRF depth) to preferentially photobleach fluorescence f

106 e features of this ultra-sensitive liposomal TIRF-FOB are (i) fluorescence is excited via evanescent

107 ce fluorescence protein-binding microarrays (TIRF-PBM) to evaluate the effects of protein phosphoryla

108 fluorescence and scanning force microscope (TIRF-SFM) to pinpoint fluorescently labeled human homolo

109 internal reflection fluorescence microscopy (TIRF) that a proportion of ARHGAP18 localizes to microtu

110 internal reflection fluorescence microscopy (TIRF).

111 cence microscopy (vaTIRFM) adapted to modern TIRF setup.

112 tly to Daam1, and multicolor single-molecule TIRF imaging revealed that fascin recruited Daam1 to and

113 Using single-molecule TIRF imaging, we have measured the affiliation of GLP-1

114 he low signal/noise ratio in single-molecule TIRF microscopy experiments, it is important to determin

115 Here we use single-molecule TIRF microscopy in living cells to reveal that the enzym

116 We show, using a single-molecule TIRF microscopy technique, that the exchange process is

117 structed from several layers within a normal TIRF excitation zone by sequentially imaging and photobl

118 Here, we use a combination of TIRF excitation and supercritical angle fluorescence emi

119 n of DNA standards to quantify the limits of TIRF-FRET resolution.

120 that SAF improves the surface selectivity of TIRF, even at shallow penetration depths.

121 chnique involves the recording of a stack of TIRF images, by gradually increasing the incident angle

122 solution using modern widefield, confocal or TIRF microscopes with illumination orders of magnitude l

123 cence structured-illumination microscopy, or TIRF-SIM, to visualize individual myosin II bipolar fila

124 the case of NMIIB-HMM in optical tweezers or TIRF/in vitro motility experiments.

125 estimator is thus suited for single-particle TIRF microscopy of dense biological samples in which the

126 Single-particle TIRF microscopy shows that wild-type channels in PM have

127 red by monitoring intensity of the polarized TIRF images.

128 Yet truly quantitative TIRF remains problematic.

129 pyrene-actin and total internal reflection (TIRF) microscopy elongation assays.

130 ontrast (RIC) and total internal reflection (TIRF) microscopy, respectively.

131 g single-molecule total internal reflection (TIRF) microscopy, we have examined the assembly and disa

132 work paves the way for ultra-high-resolution TIRF-FRET studies on many biomolecules, including DNA pr

133 gle was tuned from the highest (the smallest TIRF depth) toward the critical angle (the largest TIRF

134 azimuthal and polar beam scanning (spinning TIRF), atomic force microscopy, and wavefront analysis o

135 ts with subcellular resolution on a standard TIRF microscope, with a removable Bertrand lens as the o

136 SW-TIRF is a wide-field superresolution technique with reso

137 e total internal reflection fluorescence (SW-TIRF).

138 esults confirm the superior resolution of SW-TIRF in addition to the merit of a high signal/backgroun

139 We demonstrate the performance of the SW-TIRF microscopy using one- and two-directional SW illumi

140 expression immobilized GLUT4 vesicles in the TIRF zone and promoted insulin-induced GLUT4 exposure to

141 ion, increased GLUT4 vesicle velocity in the TIRF zone, and prevented their externalization.

142 Reconstruction of the TIRF images enabled 3D imaging of biological samples wit

143 d high-numerical aperture aberrations of the TIRF objective as one important source.

144 microtubule density and curvature within the TIRF-illuminated region of the cell.

145 Data presented here from real-time TIRF (TIRFM) and confocal microscopy coupled with surfac

146 ce (TIRF) Forster resonance energy transfer (TIRF-FRET) microscopy allows multiple biomolecules to be

147 the excitation impurities in objective-type TIRF are only weakly affected by changes of azimuthal or

148 far-field excitation light in objective-type TIRF, at least for most types of weakly scattering cells

149 n light that is contaminating objective-type TIRF.

150 anted far-field excitation in objective-type TIRF. Pt.1. Identifying sources of nonevanescent excitat

151 We then use TIRF-FRET to monitor the behavior of individual insulin-

152 We use TIRF microscopy to show that cortactin bundles branched

153 Here we used TIRF and electron microscopy to directly compare for the

154 We used TIRF microscopy to directly observe actin filament sever

155 Using TIRF microscopy at the single molecule level, transient

156 in the plasma membrane of living cells using TIRF microscopy.

157 ents on membrane-bound actin filaments using TIRF microscopy.

158 in vitro analysis of the K118M mutant using TIRF microscopy indicates the actual number of branches

159 also visualized at the cell periphery using TIRF microscopy.

160 alpha6- and beta3-containing receptors using TIRF.

161 we developed a single molecule system using TIRF (total internal reflection fluorescence) microscopy

162 near-infrared optical tweezers combined with TIRF microscopy, we were able to trap peroxisomes and ap

163 plifier and sandwich immunoassay format with TIRF-FOB.

164 Here, we combine optical trapping with TIRF-based microscopy to measure the force dependence of