ライフサイエンスコーパス: TPH

1 TPH cells appear to be uniquely poised to promote B-cell

2 TPH is protected from dopamine-induced inactivation by r

3 TPH message levels doubled between early day and early n

4 TPH mRNA was elevated during pregnancy and lactation, an

5 TPH was induced by PRL in mammosphere cultures and by mi

6 TPH-IR (microCi/g) was higher in suicides than controls

7 TPH-IR, an index of the amount of TPH enzyme, in the DRN

8 in, synthesized by tryptophan hydroxylase-1 (TPH(1)), has been shown to play a key role in several ph

9 We found that 1) TPH and SERT immunoreactivity in neurons of raphe magnus

10 followed by a gradual decline until P21; 3) TPH labeling in neurons of the ventrolateral medullary s

11 except for a significant fall at P12; and 4) TPH and SERT immunoreactivity in a number of other nucle

12 Redox cycling of a TPH-quinoprotein could also participate in the serotonin

13 on, H2O2, or tyrosinase alone does not alter TPH activity.

14 carbons (TPH) were 18 +/- 0.6 g/kg soil, and TPH carbon constituted approximately 40% of the dichloro

15 Apo-TPH exposed to NO cannot be reactivated by iron.

16 been proposed for specific polymorphisms at TPH (suicide-related behaviors and impulsivity), DRD3 (s

17 Because TPH mRNA are regulated by the endogenous pineal circadia

18 ranscriptomics highlight differences between TPH cells and T follicular helper cells, including alter

19 +P) using in situ hybridization and a 249 bp TPH cRNA probe generated with RT-PCR (n = 5 animals/grou

20 We have expressed brain TPH as a recombinant glutathione-S-transferase fusion pr

21 ins form a complex with phosphorylated brain TPH, thereby increasing its enzymatic activity and inhib

22 PD-1(hi)CXCR5(+) T follicular helper cells, TPH cells induce plasma cell differentiation in vitro th

23 d the percentage of PR-ir cells colocalizing TPH-ir in both raphe nuclei, regardless of sex and genot

24 lted in lower, more statistically comparable TPH values.

25 o (112 +/- 22) of a non-releasing composite (TPH) (p > 0.1).

26 n of nuclear ER-ir or PR-ir with cytoplasmic TPH-ir or GFAP-ir was observed in either sex or treatmen

27 DRN TPH-IR was higher in male suicide victims (MS) compared

28 l requires the serotonin biosynthetic enzyme TPH-1 in neurons and the serotonin receptor SER-7 in the

29 e presence of 5-hydroxytryptophan, epidermal TPH activity is completely absent.

30 ence of RANKL, osteoclast precursors express TPH(1) and synthesize serotonin.

31 We previously reported that, in Finns, TPH genotype was associated with suicidality, a pathophy

32 -dependent reduction in 5-HIAA, a marker for TPH inhibition, from baseline until week 4.

33 trahydrobiopterin (BH(4)) and tryptophan for TPH NDelta15 were not observed.

34 Cultures from TPH(1)(-/-) in the presence of macrophage colony-stimula

35 Removal of iron from TPH produces an apoenzyme with low activity that can be

36 Treatment of holo-TPH (iron-loaded) with the disulfide 5,5'-dithio-bis (2-

37 zed to generate a full-length model of human TPH.

38 sults show that total petroleum hydrocarbon (TPH) removal rate almost doubled in soils close to the a

39 Total petroleum hydrocarbons (TPH) as a lumped parameter can be easily and rapidly mea

40 atment reduced total petroleum hydrocarbons (TPH) to below regulatory standards (typically <1% by wei

41 Total petroleum hydrocarbons (TPH) were 18 +/- 0.6 g/kg soil, and TPH carbon constitut

42 ymes, 5'-end triphosphonucleotide hydrolase (TPH) instead of PPH activity.

43 substrate availability and TRP hydroxylase (TPH) enzymatic activity, leading to accumulation of exog

44 ther this depends on tryptophan hydroxylase (TPH) 1, the critical enzyme for peripheral 5-HT synthesi

45 , the mRNA levels of tryptophan hydroxylase (TPH) 1, TPH2 (both are involved in serotonin synthesis),

46 Using tryptophan hydroxylase (TPH) 2 deficient (Tph2-deficient) mice that lack brain s

47 for 5-HT synthesis, tryptophan hydroxylase (TPH) and aromatic amino acid decarboxylase (AADC) are ex

48 -synthesizing enzyme tryptophan hydroxylase (TPH) and serotonin transporter (SERT) with semiquantitat

49 biosynthetic enzyme tryptophan hydroxylase (TPH) are poorly understood.

50 Rabbit brain tryptophan hydroxylase (TPH) has been expressed in insect cells (Spodoptera frug

51 class of peripheral tryptophan hydroxylase (TPH) inhibitors is described.

52 otonin biosynthesis, tryptophan hydroxylase (TPH) is a potential target for this autoregulation.

53 Tryptophan hydroxylase (TPH) is the initial and rate-limiting enzyme in the bios

54 Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in serotonin biosynthes

55 Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the synthesis of 5-H

56 Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the synthesis of ser

57 Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the synthesis of the

58 pression of mRNA for tryptophan hydroxylase (TPH) was examined in ovariectomized (spayed) control, E-

59 biosynthetic enzyme tryptophan hydroxylase (TPH) was expressed in nearly 10% of neurons following tr

60 l Trp metabolism via tryptophan hydroxylase (TPH) with its downstream cascade, including serotonin an

61 hydroxylase (TH) and tryptophan hydroxylase (TPH), and draw an evolutionary scenario for this cell po

62 sis of CART and CART+tryptophan hydroxylase (TPH), CART+estrogen receptors (ER) or CART+progesterone

63 t in neuropsychiatry-tryptophan hydroxylase (TPH), dopamine transporter protein (SLC6A3), D3 dopamine

64 synthesizing enzyme, tryptophan hydroxylase (TPH), or to the astrocytic marker, glial fibrillary acid

65 at the mRNA encoding tryptophan hydroxylase (TPH), the first enzyme in the melatonin synthetic pathwa

66 Exposure of tryptophan hydroxylase (TPH), the initial and rate-limiting enzyme in the biosyn

67 Tryptophan hydroxylase (TPH), the initial and rate-limiting enzyme in the biosyn

68 gic markers, such as tryptophan hydroxylase (TPH)- or 5-HT-positive cells and TPH2 RNA levels, were u

69 ing in the number of tryptophan hydroxylase (TPH)-positive neurons in the DR of wild-type (WT) mice.

70 olecule inhibitor of tryptophan hydroxylase (TPH).

71 synthesizing enzyme, tryptophan hydroxylase (TPH).

72 t -7/-10 kb in human tryptophan hydroxylase (TPH)2 were probed.

73 otonin biosynthesis (tryptophan hydroxylase [TPH] and aromatic amine decarboxylase).

74 etermine the amount of TPH immunoreactivity (TPH-IR) in the dorsal (DRN) and median (MRN) raphe nucle

75 arly day produced a modest increase (50%) in TPH message levels but had no effect at other times.

76 in the synthesis of 5-HT, and alterations in TPH could explain some of these findings.

77 ed sites, such as CCR2, CX3CR1, and CCR5, in TPH cells.

78 em nuclei exhibited a significant decline in TPH and SERT immunoreactivity during the critical period

79 oradiographs revealed a ninefold increase in TPH mRNA in E-treated macaques compared to spayed animal

80 mal-looking neurons and a marked increase in TPH-positive spindle-shaped cells.

81 ones covalently modify cysteinyl residues in TPH, leading directly to the loss of catalytic activity,

82 ence that the decrease in bone resorption in TPH(1)(-/-) mice is cell-autonomous.

83 Bone resorption in TPH(1)(-/-) mice, as assessed by biochemical markers and

84 neurotoxic to 5-HT neurons, that inactivate TPH in vivo and are now known to produce NO and other re

85 detection (HPLC-EC) analysis of inactivated TPH revealed the formation of cysteinyl-dopamine residue

86 These data indicate that NO inactivates TPH by selective action on critical SH groups (i.e., cys

87 ort of O3 to TPH bound to soil and increased TPH removal.

88 These data indicate that E induces TPH gene expression in nonhuman primates and that the ad

89 t the benchmark dose of 3.4 kg O3/kg initial TPH, TPH carbon was reduced by nearly 6 gC/kg soil (40%)

90 eurons and CART fibers appeared to innervate TPH-positive serotonin neurons in the dorsal raphe.

91 oautoradiography, using an antibody to label TPH, was performed, slides exposed to film and autoradio

92 autoreceptor agonist, CGS 12066A, can lower TPH mRNA levels and promoter activity.

93 detail bone remodeling in growing and mature TPH(1) knockout mice (TPH(1)(-/-)).

94 in growing and mature TPH(1) knockout mice (TPH(1)(-/-)).

95 Exposure of dopamine-modified TPH to redox-cycling staining after SDS-PAGE confirmed t

96 More TPH may be an upregulatory homeostatic response to impai

97 Moreover, TPH was enzymatically active (112.8+/-36 pmol/mg per h)

98 The inactivation of native TPH by NO cannot be reversed by either iron or DTT.

99 TPH-IR, an index of the amount of TPH enzyme, in the DRN is higher in depressed suicides.

100 We sought to determine the amount of TPH immunoreactivity (TPH-IR) in the dorsal (DRN) and me

101 The analysis of TPH-IR area and density at each DRN rostrocaudal levels

102 ther dissect the regulation and catalysis of TPH.

103 This class of TPH inhibitors exhibits excellent potency in in vitro bi

104 the method and improve the comparability of TPH data, crucial GC-based quantification issues were ex

105 The dephosphorylation of TPH by protein phosphatase-1 was inhibited by 14-3-3 pro

106 es a concentration-dependent inactivation of TPH as well.

107 nzoic acid) (DTNB) causes an inactivation of TPH that is readily reversed by dithiothreitol (DTT).

108 hat cause dopamine-dependent inactivation of TPH.

109 ce is consistent with a greater induction of TPH 2, and 5-HTT by EB in DRN that play key roles in emo

110 The pharmacological inhibition of TPH activity blocked these effects.

111 Local inhibition of TPH in the gastrointestinal tract might reduce mucosal p

112 The inherent instability of TPH has prevented a crystallographic structure from bein

113 ies that distinguish between the isoforms of TPH will allow studies of the differential regulation of

114 are effective in preventing the labeling of TPH by [3H]dopamine.

115 ion is probably not mediated at the level of TPH gene transcription.

116 forskolin+TPA) yielded the greatest level of TPH induction (15.6%).

117 e significantly higher single-cell levels of TPH mRNA in serotonergic neurons of the dorsal raphe in

118 Heretofore, probes used for localization of TPH protein or mRNA could not distinguish between the TP

119 here was a marked reduction in the number of TPH-positive normal-looking neurons and a marked increas

120 We have found that phosphorylation of TPH by cAMP-dependent protein kinase increased the activ

121 ns play important roles in the regulation of TPH activity.

122 Regulation of TPH via microphthalmia-associated transcription factor a

123 hat Tyr235 (Y235), within the active site of TPH, appears to be involved as a tryptophan substrate or

124 the regulation and substrate specificity of TPH, as well as providing a basis to understand as yet t

125 addition of P has little additive effect on TPH gene expression.

126 roaches did not have a significant effect on TPH results.

127 d the hydroxylase activity of phosphorylated TPH by approximately 45%.

128 that 14-3-3 proteins bind to phosphorylated TPH with an affinity constant (Ka) of 4.5 x 10(7) M-1.

129 14-3-3 proteins interact with phosphorylated TPH.

130 Once thought to be a single-gene product, TPH is now known to exist in two isoforms-TPH1 is found

131 educing agents and antioxidants that protect TPH from inactivation are effective in preventing the la

132 Highly purified TPH catalytic core, like the native enzyme from brain, i

133 statistical analyses show that the residual TPH has a strongly positive correlation with hydrocarbon

134 terval, resulting in the order CEN-LDHA-SAA1-TPH-D11S1310-(D11S1888/KCNC1 )-MYOD1-D11S902D11S921-D11S

135 the amino terminal autoregulatory sequence (TPH NDelta15).

136 n of PD-1(hi)CXCR5(-) 'peripheral helper' T (TPH) cells that express factors enabling B-cell help, in

137 ss of catalytic activity, and establish that TPH could be a target for dopamine-quinones in vivo afte

138 -specific, because MEKK did not activate the TPH promoter in nonneuronal cell lines.

139 in or mRNA could not distinguish between the TPH isoforms because of extensive homology shared by the

140 The intron 7 variant in the TPH gene showed significant evidence for linkage to suic

141 The association of the TPH 17 779C (L) allele to suicidality in impulsive offen

142 The status of the TPH A779C allele as a marker for suicidality was replica

143 nalyses of a polymorphism in intron 7 of the TPH gene with suicidality, alcoholism, and the Karolinsk

144 The amplitude of the TPH mRNA rhythm was increased to 4-fold by culturing the

145 repression of MAP kinase stimulation of the TPH promoter.

146 od PetroFLAG) were performed to quantify the TPH content in samples collected from a site contaminate

147 st this hypothesis, we first showed that the TPH promoter can be activated 20-fold by mitogen-activat

148 A functional variant(s) in or close to the TPH gene may predispose individuals to suicidality and o

149 in the soil improved mass transport of O3 to TPH bound to soil and increased TPH removal.

150 benchmark dose of 3.4 kg O3/kg initial TPH, TPH carbon was reduced by nearly 6 gC/kg soil (40%), whi

151 DTNB-treated TPH [sulfhydryl (SH)-protected] exposed to NO is returne

152 With supplemental P treatment, TPH mRNA signal was increased fivefold over spayed anima

153 uced specific activity compared to wild-type TPH ( approximately 5 % residual activity).

154 ignificantly increased compared to wild-type TPH (42 microM).

155 kinetic analyses were performed on wild-type TPH and a deletion construct that lacks the amino termin

156 ere not phosphorylated, and unphosphorylated TPH was not activated by 14-3-3 proteins.

157 PR-ir or ER alpha-ir often colocalized with TPH-ir.