ライフサイエンスコーパス: TREC

1 TREC levels correlated with the frequency of phenotypica

2 TREC levels rose weeks after HSCT and could be detected

3 TREC NBS in California has achieved early diagnosis of S

4 TREC numbers can be measured in DNA isolated from the dr

5 TREC test specificity was excellent: only 0.08% of infan

6 TREC testing of newborns is now being performed in sever

7 TRECs peaked earlier and with higher values (P <.01) in

8 TRECs were present in all patients in both total lymphoc

9 ance judgments produced in the 2004 and 2005 TREC Genomics Track and observe significant correlations

10 In patient 8 TREC levels were borderline and KREC levels were abnorma

11 and KREC levels were abnormal; in patient 9 TRECs were undetectable, whereas KREC levels were normal

12 ble TRECs and might therefore be missed by a TREC-based newborn screening program.

13 vels and will therefore not be detected in a TREC-based newborn screening program.

14 nt superimposed onto the trapping rings of a TREC.

15 data from 3,030,083 newborns screened with a TREC test.

16 37 weeks' gestation had at least 1 abnormal TREC assay (TREC values < 25/microL), 11 of whom had sam

17 Infants with low or absent TRECs can thus be identified and referred for confirmato

18 Additionally, TRECs were detected in genomic DNA obtained from Guthrie

19 l division have the same potential to affect TREC concentration at any age in healthy people.

20 However, not all TREC decreases could be attributed to increased T-cell p

21 mance rankings generated by our approach and TREC.

22 over of memory CD4(+) and CD8(+) T-cells and TREC depletion in naive CD4(+) T-cells, although naive T

23 w cytometry), in vitro responses to PHA, and TREC levels, all measured at presentation, were compiled

24 Naive CD4(+) lymphocyte phenotype and TREC levels were not significantly different in patients

25 d with published data on Ki67 expression and TRECs within naive CD4+ T cells in healthy individuals.

26 The TREC test has clinical validity and TRECs, as predicted, are an excellent biomarker of poor

27 Recent research initiatives such as TREC Genomics and BioCreAtIvE strongly point to the meri

28 estation had at least 1 abnormal TREC assay (TREC values < 25/microL), 11 of whom had samples analyze

29 Thymic function failure (sj/beta-TREC ratio <10) was independently associated with HIV pr

30 cell rearrangement excision circles (sj/beta-TREC ratio) overcomes this limitation but has only been

31 ance of thymic function, measured by sj/beta-TREC ratio, in HIV disease progression by analyzing a la

32 of thymic function, measured by the sj/beta-TREC ratio, on CD4 T-cell maintenance in prospective HIV

33 n peripheral blood samples using the sj/beta-TREC ratio.

34 ths after bone marrow transplantation (BMT), TREC levels remained low for 3 months after BMT.

35 t ADA deficiency had normal TREC levels, but TRECs were undetectable in blood samples taken from the

36 that thymectomy decreased CD4 or CD8 T cell TREC concentrations most when thymopoiesis was active be

37 no significant mean difference in PB T cell TREC levels between ages 40 and 80 years.

38 hymectomized MG patients had lower PB T cell TREC levels than did age-matched normal subjects (p < 0.

39 ell called the trapping ring electrode cell (TREC) has been conceived, simulated, developed, and test

40 applied to the Trapping Ring Electrode Cell (TREC).

41 ometry) and T-cell receptor excision circle (TREC) abundance.

42 notypic and T cell receptor excision circle (TREC) analysis confirmed a recent thymic origin of the e

43 Using T-cell receptor excision circle (TREC) analysis, we directly measured in vivo replicative

44 ening using T-cell receptor excision circle (TREC) analysis.

45 T-cell receptor excision circle (TREC) and kappa-deleting recombination excision circle (

46 D using the T-cell receptor excision circle (TREC) assay began in Wisconsin in 2008; 5 infants with S

47 T-cell receptor excision circle (TREC) assays for the evaluation of thymus activity revea

48 elopment of T-cell receptor excision circle (TREC) assays of thymopoiesis, cytokine-flow cytometry as

49 TCR excision circle (TREC) content has been used as a proxy for thymic export

50 cell receptor rearrangement excision circle (TREC) levels of 2000 or more copies/mug DNA.

51 typing, and T cell receptor excision circle (TREC) levels were measured.

52 signal joint (sj) TCR delta excision circle (TREC) levels, a molecular marker for active thymopoiesis

53 ession, and T cell receptor excision circle (TREC) measurements in T cells were evaluated at weeks 0

54 , including T-cell receptor excision circle (TREC) quantification, in patients with MHC-II deficiency

55 7 staining, T-cell receptor excision circle (TREC) quantitation in sorted CD4 and CD8 cells, and thym

56 results of T-cell receptor excision circle (TREC) testing newborns in pilot states indicate that thi

57 e counts of T cell receptor excision circle (TREC)-containing CD4 T cells (presumed recent thymic emi

58 numbers of T-cell receptor excision circle (TREC)-positive T cells, indicating increased thymopoiesi

59 evels of TCR rearrangement excision circles (TREC) and parameters of cell proliferation, including Ki

60 TCR excision circles (TREC) as a marker of thymic output exponentially decreas

61 heir lower T-cell receptor excision circles (TREC) content and shorter telomeres proved they had divi

62 T cell receptor excision circles (TREC) have been recently used to assess thymic output du

63 Assays for T cell receptor excision circles (TREC) have been utilized in human, primate, and mouse mo

64 T cell repertoire, low TCR excision circles (TREC) values, and a predominance of CD45RO(+) T cells.

65 Levels of T receptor excision circles (TREC) were comparable in purified CD4(+)CD25(+) and CD4(

66 ell receptor rearrangement excision circles (TREC) were substantially reduced in RA patients; TREC le

67 ell receptor rearrangement excision circles (TREC) within peripheral T cell populations provides insi

68 h level of T-cell receptor excision circles (TREC), demonstrating de novo T lymphopoiesis, and functi

69 of macaque T-cell receptor excision circles (TREC).

70 itation of T-cell receptor excision circles (TRECs) and kappa-deleting recombination excision circles

71 levels of T-cell receptor excision circles (TRECs) and the lowest frequencies of Ki67(+) T cells, wh

72 eceptor gene rearrangement excision circles (TRECs) as a measure of recent thymic emigrant cells in p

73 to detect T-cell receptor excision circles (TRECs) began in Wisconsin in 2008, and SCID was added to

74 ell receptor rearrangement excision circles (TRECs) declined from 47,946 to 26,510 copies per 10(6) n

75 ell-receptor rearrangement excision circles (TRECs) decreased.

76 ceptor (TCR) rearrangement excision circles (TRECs) have been used as markers for recent thymic emigr

77 Assay of T-cell receptor excision circles (TRECs) in dried blood spots obtained at birth permits po

78 ell receptor rearrangement excision circles (TRECs) in human thymus.

79 ell receptor rearrangement excision circles (TRECs) in measles patients were increased in CD8+ T cell

80 urement of T-cell receptor excision circles (TRECs) in peripheral blood is a means of quantifying rec

81 ication of T-cell receptor excision circles (TRECs) or tandem mass spectrometry (tandem-MS) analysis

82 ilution of T cell receptor excision circles (TRECs) or the dye CFSE, and clonal expansion has been do

83 rations of T-cell-receptor excision circles (TRECs) were 10 per 100 T cells.

84 function, T-cell receptor excision circles (TRECs) were examined in CD4(+) and CD8(+) cells from a c

85 nd T-cell antigen receptor excision circles (TRECs) were measured before transplantation and sequenti

86 cell-receptor signal joint excision circles (TRECs) were not detected in reconstituting T cells in do

87 esting for T-cell receptor excision circles (TRECs), a DNA biomarker of normal T-cell development, ha

88 Finally, no TRA/delta excision circles (TRECs), a marker of TRA/delta locus rearrangements, were

89 ing signal joint TCR delta excision circles (TRECs), a molecular marker of thymus emigrants that have

90 ertoire, TCR rearrangement excision circles (TRECs), and hematopoietic chimerism were studied in the

91 T-cell receptor excision circles (TRECs), markers of T cells generated de novo, were quant

92 tive TCRalpha joins on TCR excision circles (TRECs), which are the products of secondary V/J recombin

93 ell receptor rearrangement excision circles (TRECs).

94 urement of T cell receptor excision circles, TRECs), and studies of thymus biopsies in untreated and

95 tandem repeat endonuclease coupled cleavage (TREC) with yeast as an intermediary host for the genome

96 ified therapeutic receptor-effector complex (TREC) suitable for gene therapy.

97 in the Text Retrieval Evaluation Conference (TREC), organized annually for the past 15 years, to supp

98 hermally regenerative electrochemical cycle (TREC) is an attractive approach which uses the temperatu

99 mized mice resulted in patterns of decreased TREC frequency and increased total TREC number similar t

100 rd blood grafts had no evidence of decreased TREC induced by immunosuppressive prophylaxis drugs.

101 ients with MHC-II deficiency have detectable TRECs and might therefore be missed by a TREC-based newb

102 At discharge, TRECs in CD4+ T cells (P = 0.05) and circulating levels

103 uggested that IL-7 can also directly enhance TREC generation.

104 mpared to CD4(+), CD4(-) iNKTs contain fewer TRECs, express higher levels of IL-2Rbeta, and prolifera

105 At baseline, patients exhibited fewer TRECs than did uninfected control subjects.

106 evaluation of thymus activity revealed fewer TRECs in both transplant groups compared with healthy do

107 In this study, a quantitative assay for TRECs was used to measure T-cell neogenesis in adult pat

108 peat DNA isolation with quantitative PCR for TRECs and a genomic control, and immunophenotyping was p

109 Here, we demonstrate a charging-free TREC consisting of an inexpensive soluble Fe(CN)6(3-/4-)

110 This charging-free TREC system may have potential application for harvestin

111 arily evaluated using the Genomics data from TREC (Text Retrieval Conference) 2006 Genomics Track.

112 Furthermore, TRECs correlated positively with the number of CD4 and n

113 s isolated from peripheral blood had greater TREC quantities than their CD4(+)CD25(+) counterparts, s

114 Finally, 51 patients had TREC levels measured.

115 ell output by the thymus as revealed by high TREC values and a polyclonal T cell repertoire demonstra

116 ipients of matched sibling grafts had higher TREC levels.

117 n the number of RTE generated per day and in TREC concentration during an 80-year lifespan.

118 t is the major contributor to the decline in TREC concentration within CD4(+) T cells, whereas both i

119 ecreased thymic output induce the decline in TREC concentration within CD8(+) T cells.

120 rimarily induces an age-dependent decline in TREC concentrations within both CD4(+) and CD8(+) T cell

121 rus (HIV)-infected patients, this decline in TREC levels did at times correlate with an increased lev

122 rapid viral recrudescence and a decrease in TREC levels.

123 Decreases in TREC frequency were attributable to dilution secondary t

124 c suppression) also had substantial gains in TRECs.

125 The increase in TRECs after antiretroviral therapy was greater in infant

126 mmune organs, accounting for the increase in TRECs in the total peripheral lymphoid pool; and (3) no

127 caques (n = 23) revealed stable or increased TREC levels at 20 to 34 weeks postinfection.

128 et of methods with data from two independent TREC Genomics Track evaluations.

129 T cell death also influences TREC concentration, but to a lesser extent.

130 The development of the porcine signal joint TREC assay should enable a more direct quantification of

131 hain reaction (PCR) to quantify signal-joint TRECs (sjTRECs) before and after transplantation.

132 Low TREC-containing CD4 T cell counts were associated with o

133 Omenn syndrome, and ZAP70 deficiency had low TREC levels.

134 Samples with initial low TREC numbers had repeat DNA isolation with quantitative

135 the most important factor that predicted low TREC levels even years after HSCT.

136 At week 0, NRs had lower TREC levels per 1x106 T cells and higher levels of T cel

137 ipheral effects of IL-7 on RTEs, we measured TREC content and peripheral naive T-cell subsets and tur

138 Most TREC systems still require external electricity for char

139 culating T cell numbers, particularly naive (TREC+) T cells.

140 with delayed-onset ADA deficiency had normal TREC levels, but TRECs were undetectable in blood sample

141 R222C mutation in the IL2RG gene, had normal TREC levels.

142 nts with conditions including TD have normal TREC levels and will therefore not be detected in a TREC

143 monstrate (1) that total TREC number and not TREC frequency accurately reflects quantitative changes

144 Tandem-MS but not TREC quantification identifies newborns with delayed- or

145 eoretical limit was achieved using the novel TREC technology; over 420,000 resolving power was observ

146 played thymic regeneration and attainment of TREC levels of 2000 or more copies/mug DNA (P = .005).

147 m), CD95(dim)) and its high concentration of TREC.

148 The counts of TREC(+) CD4 T cells in transplant recipients younger tha

149 In contrast, the counts of TREC(+) CD4 T cells were lower in transplant recipients

150 A decline of TREC levels and a decrease in the number of CD45RA(+) ce

151 In summary, reduced levels of TREC can be observed beginning at 16 to 30 weeks post-SI

152 naive CD8(+) cells display higher levels of TREC than their CD103(-) naive counterparts, and these c

153 This suggests that the loss of TREC during HIV infection can arise from a combination o

154 feration did not increase, despite a loss of TREC within naive CD4+ T cells.

155 The loss of TREC-positive T cells could be a consequence of a primar

156 Simultaneous measurement of TREC and KREC copy numbers in Guthrie card samples readi

157 er relative change (n-fold) in the number of TREC+ T cells/mul than in naive T-cell counts was observ

158 ry of resolved GVHD had decreased numbers of TREC, compared with those with no GVHD.

159 Stability of TREC frequencies throughout the period of repertoire gen

160 We sought to report the first 2 years of TREC NBS in California.

161 on, whereas the other tracks the dynamics of TRECs.

162 ce, our analysis showed a lower frequency of TRECs with male-reactive V17J57 joins in male mice.

163 ction demonstrated that the highest level of TRECs (14 692 copies/10 000 cells) was present in the CD

164 Measurement of TRECs may serve as a useful tool for evaluating immune r

165 ll lymphopenia by quantitating the number of TRECs contained in a 3.2-mm punch (approximately 3 micro

166 as also associated with increased numbers of TRECs in CD3(+) T cells (P <.001) and with conversion to

167 hymic activity, which was estimated based on TREC levels and T-cell receptor (TCR) genes, as well as

168 requency of in-frame, male-specific joins on TRECs in male and female HYbeta transgenic mice.

169 As a general paradigm, TRECs for enhancement of other G-protein signaling appea

170 ) were substantially reduced in RA patients; TREC levels in young adult patients matched those of con

171 poiesis resulted in a significant fall in PB TREC(+) T cells postthymectomy.

172 ted why previous attempts at cloning the pig TREC using known sjTREC sequences were unsuccessful.

173 of T-cell receptor excision circle-positive (TREC(+)) cells in the peripheral blood and dramatic incr

174 Unlike in the detection of primate TRECs, initially the use of similar primers close to the

175 deoxyadenosine and real-time PCR to quantify TREC levels.

176 evaluate whether tandem-MS and quantitative TREC analyses of DBSs could identify newborns who had de

177 Here we use the topographic and recognition (TREC) mode of an atomic force microscope to visualize UC

178 in some patients was associated with reduced TREC levels, but infusion of mature donor CD4(+) T cells

179 development of the porcine signal joint (sj) TREC assay, and provide a likely reason for previous dif

180 We further apply this model for studying TREC concentration during HIV-1 infection.

181 IV-infected macaques (n = 4) determined that TREC levels decreased between 24 and 48 weeks postinfect

182 his approach is complicated by the fact that TREC levels also are determined by turnover within the n

183 8(+) stage in native thymus, suggesting that TREC generation occurred following the cellular division

184 The TREC Genomics Track has recently been introduced to meas

185 The TREC permits the ability to maintain coherent ion motion

186 The TREC test has clinical validity and TRECs, as predicted,

187 Exactly 71,000 infants were screened by the TREC assay.

188 on efficiency of 2.0% can be reached for the TREC operating between 20 and 60 degrees C.

189 Furthermore, the TREC did not display tachyphylaxis to prolonged agonist

190 culture system, exogenous IL-7 increased the TREC frequency in fetal as well as infant thymus, indica

191 In a statewide screening program, use of the TREC assay performed on NBS cards was able to identify i

192 The performance of the TREC is compared to a closed cylindrical cell at differe

193 This review will provide an overview of the TREC screening assay and an update of the findings from

194 a number of reranking experiments using the TREC 2005 genomics track test collection in which scores

195 newborn population-based screening using the TREC assay, including the evaluation and care of infants

196 Routine screening of all newborns with the TREC test, implemented as part of an integrated public h

197 division remains relatively unchanged, then TREC concentration indeed reflects thymic output.

198 A high-throughput TREC quantitative PCR assay with DNA isolated from routi

199 decreased TREC frequency and increased total TREC number similar to those in IL-7-treated thymus-inta

200 ymus-intact mice resulted in increased total TREC numbers, consistent with RTE accumulation.

201 These results demonstrate (1) that total TREC number and not TREC frequency accurately reflects q

202 During phase 2 (after 16 to 30 weeks), TREC levels declined in both T-cell populations.

203 During phase 1 (16 to 30 weeks), TREC levels remained stable or increased within both the

204 tifies newborns with PNP deficiency, whereas TREC or KREC measurement alone can fail.

205 and FoxP3 expression on CD4(+) T cells, with TREC levels per 1x106 CD4(+) T cells decreasing signific

206 whether age alone inversely correlated with TREC levels.

207 ivalent of at least 37 weeks' gestation with TREC values of less than 25/microL.

208 ng bpref measured with NT Evaluation or with TREC evaluations.

209 positively correlates in these patients with TREC levels.

210 ll turnover should be examined together with TREC concentration as a measure of RTE.

211 After the age of 70 years, TRECs only slightly declined, but homeostatic proliferat