ライフサイエンスコーパス: TdT

1 TdT addition of digoxigenin labeled nucleotides to 3' OH

2 TdT adds nontemplated N nucleotides to the junctions of

3 TdT and an Ig heavy chain transgene were detected within

4 TdT expression suggests the presence of immature B cells

5 TdT expression was found in several samples, but did not

6 TdT is a DNA polymerase that plays a major role in gener

7 TdT is a nuclear enzyme that catalyzes the addition of r

8 TdT staining indicated that following 2-MeOE2-mediated i

9 TdT's ability to incorporate fluorescent dNTPs into a ss

10 TdT(+) cells found within the tonsillar fibrous scaffold

11 TdT-mediated dUTP nick end labeling assay at 48 h indica

12 TdT-mediated dUTP nick-end labeling (TUNEL) assay was us

13 TdT-mediated nick end-label (TUNEL) staining showed apop

14 TdT-mediated synthesis may be a useful approach for crea

15 and included most RAG-1+ ELP and many RAG-1- TdT+ ELP.

16 ecombinase-activating gene-1 (RAG-1), RAG-2, TdT, Ig mu, lambda 5, and VpreB.

17 18 sequences from adult MRL/lpr- and C57BL/6 TdT-deficient B cell precursors and found only two examp

18 ins no TATA sequence, but instead contains a TdT-like initiator element (Inr) at nucleotides -3 to +5

19 d selection and suggest the possibility of a TdT-deficient precursor population in the adult BM.

20 was demonstrated in 5H7-treated PBMC using a TdT-mediated end-labeling (TUNEL) technique.

21 ha 1-->3 dextran [DEX]) by challenging adult TdT-sufficient (TdT(+/+)) and TdT-deficient (TdT(-/-)) g

22 Reconstitution of irradiated mice with adult TdT(+/+) BM reveals that the MZ can replenish N(-) B cel

23 sults suggest that Ku80 is required to allow TdT access to RAG post-cleavage complexes, providing sup

24 The ability of mu transgenes to alter TdT expression in cell lines also suggests that signalin

25 consistent with TdT-mediated N-addition, and TdT RNA is expressed exclusively at the pro-B cell stage

26 d contained transcripts of T early alpha and TdT, and 15 of 19 infant samples contained mRNA for RAG-

27 enhancer, and the 5' regions of the B29 and TdT loci.

28 CD3, RAG-1, Ikaros, CD10, and TdT transcripts were detected in the starting CD34+Lin-D

29 is was determined by both flow cytometry and TdT-dUTP terminal nick-end labeling analysis.

30 -associated defects with more 53BP1 foci and TdT-mediated dNTP nick end labeling-positive cells over

31 Histologic, immunocytochemical, and TdT-dUTP terminal nick-end labeling (TUNEL) analyses wer

32 Immunohistochemistry and TdT-mediated biotin-dUTP nick end labeling (TUNEL) were

33 g in mammalian cells: pol mu, pol kappa, and TdT.

34 The interaction between Ku and TdT is also observed in pre-T cells with endogenously ex

35 K1) cells, as evidenced by DNA laddering and TdT-mediated dUTP nick end-labeling assay.

36 ciated DNA fragmentation, DNA laddering, and TdT-mediated dUTP nick end labeling (TUNEL).

37 l) X family members (pol mu, pol lambda, and TdT, but not pol beta) contribute to junctional addition

38 avy chain minilocus pHC1 transgenic mice and TdT-deficient mice.

39 wed evidence of apoptosis (by morphology and TdT-mediated dUTP nick end labeling [TUNEL] assay).

40 on regions of the BRCT domains of pol mu and TdT support the conclusion that they participate in NHEJ

41 in reaction (PCR) was used to detect RAG and TdT transcripts from unselected and B cell-enriched syno

42 (+) T cells, coinciding with Rag1, Rag2, and TdT expression and the accumulation of V(beta)-DJ(beta)

43 tivity and the expression of RAG1, RAG2, and TdT in B-ALL patients.

44 um bromide-acridine orange nuclear stain and TdT-mediated dUTP nick-end labeling (TUNEL).

45 llenging adult TdT-sufficient (TdT(+/+)) and TdT-deficient (TdT(-/-)) gene-targeted mice, limited to

46 Using tetramer staining, TdT(-/-) and TdT(+/+) NOD mice showed similar frequencies of the diab

47 deoxynucleotidyl transferase (TdT)(+/+) and TdT(-/-) donor cells, demonstrate preferential repertoir

48 usage and CDR3 length between wild-type and TdT-/- mice.

49 s from neonatal liver and both wild-type and TdT-deficient adult bone marrow.

50 precursors in the liver and spleen of WT and TdT-transgenic mice but not in TdT(-/-) mice.

51 dition, analysis for evidence for apoptosis (TdT-dUTP terminal nick-end label [TUNEL] staining) and p

52 roliferation (Ki67) and increased apoptosis (TdT-mediated dUTP nick end labeling; TUNEL).

53 0 and involucrin), and markers of apoptosis (TdT-mediated dUTP nick end labeling (TUNEL) and anti-cas

54 n density along with pyknotic and apoptotic (TdT-mediated deoxyuridine triphosphate nick end-labeled)

55 c-Kit(-)Sca-1(high) bone marrow fraction are TdT(-), are RAG2(low), and do not display evidence for o

56 itiated a wave of cornification, assessed as TdT-mediated dUTP nick end-labeling-positive cells in st

57 majority of fluorescent nucleotides used as TdT substrates contain tethered fluorophores attached to

58 We report an association between TdT and a 55-kDa protein in lymphoid cells.

59 de evidence for a direct interaction between TdT and Ku proteins.

60 e is required for HSI and that N addition by TdT is a more effective mechanism in the induction of a

61 Although N-nucleotide addition by TdT plays a critical role in the generation of a diverse

62 s were found when the cells were analyzed by TdT-mediated dUTP nick end labeling (TUNEL).

63 ent dye-chromatin fragmentation assay and by TdT-dUTP terminal nick-end labeling (TUNEL) assay.

64 tumor cells excised from treated animals by TdT-mediated dUTP nick end labeling assay.

65 to TGF-beta1 were examined for apoptosis by TdT-mediated end-labeling and annexin staining.

66 ry, 70% of medial SMCs appeared apoptotic by TdT-mediated dUTP nick end labeling (TUNEL) analysis and

67 Cell viability was assayed by TdT-dUTP terminal nick-end labeling (TUNEL) in conjuncti

68 the nuclei of membrane-compromised cells, by TdT-dUTP terminal nick-end labeling (TUNEL) of apoptotic

69 ed zone was analyzed for keratocyte death by TdT-dUTP terminal nick-end label (TUNEL) staining and tr

70 chemically [DNA fragmentation as detected by TdT-mediated dUTP Nick-End Labeling (TUNEL) assay].

71 Apoptosis was evaluated by TdT-mediated dUTP nick end labeling immunohistochemistry

72 nation signal sequences may be influenced by TdT expression.

73 ncreased amounts of apoptosis as measured by TdT-mediated dUTP nick end labelling (TUNEL).

74 se in apoptosis (assessed morphologically by TdT-mediated dUTP nick- end labeling staining) is preced

75 Thus, the role played by TdT may be more extensive than previously thought.

76 matching of the second apparent D segment by TdT-introduced N nucleotides.

77 eavage reaction can be efficiently tailed by TdT, suggesting that the RAG proteins disassemble from t

78 In both cases TdT added nucleotides efficiently to the cleaved DNA end

79 e complementary determining region 3 (CDR3) (TdT(-/-)) and mice with altered Ab repertoires due to re

80 In these cells TdT protein is abundant at the time of recombination, lo

81 ciated with the RAG proteins after cleavage, TdT might be targeted for N region addition through inte

82 Thus, although differing in V(H) content, TdT-deficient mice appear to represent a good, although

83 In contrast, TdT was 3-fold upregulated in immature B cells of adults

84 TdT-sufficient (TdT(+/+)) and TdT-deficient (TdT(-/-)) gene-targeted mice, limited to the use of a si

85 ded by terminal deoxynucleotidyltransferase (TdT) activity and is not derived from DIR recombination.

86 The terminal deoxynucleotidyltransferase (TdT) gene represents an attractive model for the analysi

87 t gene terminal deoxynucleotidyltransferase (TdT) was decreased by phosphorylation of two amino acids

88 ng terminal deoxyribonucleotidyltransferase (TdT), which adds N nucleotides to V-D and D-J junctions

89 iversity that was introduced by differential TdT activities, and somatic hypermutation.

90 ns with shorter CDR3(H) were both diminished TdT activity in the DJ(H) junction and the preferential

91 ted the role of the lymphoid-specific enzyme TdT in generating B cell clones responsive to alpha-1,3

92 ded during V(D)J recombination by the enzyme TdT).

93 on of N-region additions added by the enzyme TdT.

94 such as common lymphoid progenitors express TdT and relatively high levels of RAG2, and are enriched

95 PCR analysis showed that the cells expressed TdT, lambda5, and RAG-1 genes, but that their Ig genes w

96 , like their c-kitLo counterparts, expressed TdT, proliferated in response to interleukin (IL)-7, and

97 sion of PAX5 and 72.8% of the MCCs expressed TdT.

98 ed two bone marrow (BM) subsets enriched for TdT+ cells on the brink of CD45R acquisition.

99 n and protein-DNA interactions important for TdT function in vivo.

100 This region, although not important for TdT polymerization activity, contains a BRCA1 C-terminal

101 -OH group at the nick remains obstructed for TdT-catalyzed N nucleotide addition.

102 ted at postnatal day (P) 7 and processed for TdT-dUTP terminal nick-end labeling (TUNEL) staining, an

103 apoptosis in the retina, were processed for TdT-dUTP terminal nick-end labeling (TUNEL) to determine

104 -terminal (BRCT) domain, is not required for TdT activity, although the BRCT domain clearly contribut

105 correlate well with in vitro selectivity for TdT.

106 2'-deoxynucleotides that are substrates for TdT in lymphoid precursors.

107 se-3 (CC3) activation and DNA fragmentation (TdT-mediated dUTP nick-end labeling [TUNEL]).

108 e to sort fractions B-F B lineage cells from TdT-deficient BALB/c adult BM.

109 We have generated TdT-deficient MRL/lpr, Fas-deficient (MRL-Fas(lpr)) mice

110 We have generated TdT-deficient nonobese diabetic (NOD) and MRL-Fas(lpr) m

111 Four gonococcal TdTs facilitate utilization of iron or iron chelates fro

112 erentiation of pre-pro-B cells (B220+, HSA-, TdT- or TdT+, c[mu-]) from adult rat, mouse, and human B

113 DSBs in Cos-7 cells transfected with a human TdT expression construct resulted in the appearance of d

114 Evidence for potential human TdT (hTdT) isoforms derived from hTdT genomic sequences

115 ecific J558 idiotype (Id) is not detected in TdT(-/-) mice when compared with wild-type (WT) BALB/c m

116 s demonstrating a reduced response to DEX in TdT(-/-) mice with a WT D(H) locus, we concluded that in

117 Complementation of TdT expression in TdT(-/-) mice by early forced expression of the short sp

118 vage of DEVD-AMC and blocked the increase in TdT-mediated dUTP nick end labeling (TUNEL) positivity.

119 een of WT and TdT-transgenic mice but not in TdT(-/-) mice.

120 more severely impaired HSI were observed in TdT(-/-) mice compared to those in Delta D-iD mice, whil

121 We report that in TdT-/- mice, total T(CD8+) responses to influenza and va

122 The pattern of V(H)DJ(H) usage in TdT-deficient BM largely matched that of TdT-sufficient

123 rminants are reduced to background values in TdT-/- mice while responses to three immunodominant dete

124 numbers than with standard markers including TdT-mediated dUTP biotin nick-end labeling and cleaved c

125 educed levels of DNA synthesis and increased TdT-mediated dUTP nick-end labeling staining.

126 Template-independent TdT promotes diversity during NHEJ-dependent repair of V

127 vely disrupt Ikaros binding to an integrated TdT promoter had no effect on promoter function in a CD4

128 eviously shown that newborn mice, which lack TdT due to the late onset of its expression, do not cont

129 tendency to select rearrangements with less TdT activity and shorter D segments.

130 Lin(-) TdT(+) early pro-B cells are shown here to be CD27(+) AA

131 rkers was exploited to isolate viable Lin(-) TdT(+) cells from murine bone marrow.

132 rther analysis demonstrated CD24 on most Lin-TdT+ cells and all CD45R+CD19-DX5-Ly-6C- cells.

133 Most lineage marker-negative (Lin-)TdT+ cells from murine marrow lack CD34 but display c-ki

134 itors that were Lin(-)IL-7R alpha(+)c-kit(lo)TdT(+) (lineage marker(-), interleukin receptor 7 alpha(

135 itis and increased life span in MRL-Fas(lpr) TdT(-/-) mice.

136 in the IgH CDR3 regions of the MRL-Fas(lpr) TdT(-/-) mice.

137 tested 21 MCCs for the expression of MCPyV, TdT, PAX5, IgG, IgM, IgA, kappa, and lambda by immunohis

138 To test this hypothesis, we measured TdT's ability to add nucleotides to endonuclease-induced

139 In normal mice, TdT is sharply down-regulated at the early pre-B stage i

140 ts (where H is heavy chain) in the minilocus TdT-/- mice use small portions of DIR2 located throughou

141 Furthermore, we show that DNA-PK modulates TdT activity in vitro by limiting both the length and co

142 expression was elevated in AD cases and most TdT-labeled nuclei also showed strong NT immunoreactivit

143 a structure-function analysis of the murine TdT protein to determine the roles of individual structu

144 hours, alanine transferase (ALT), necrosis, TdT-mediated dUTP-digoxigenin nick-end labeling (TUNEL)

145 and decreased pancreatic infiltration in NOD TdT(-/-) mice, and reduced glomerulonephritis and increa

146 e in CD4(+)CD25(+) regulatory T cells in NOD TdT(-/-) mice.

147 In the absence of TdT and with a defined set of human D gene segments, it

148 region 3 sequences formed in the absence of TdT are more uniform due to the use of short sequence ho

149 -Fas(lpr) mice are shorter in the absence of TdT, and there is a paucity of arginines in the IgH CDR3

150 Additionally, in the absence of TdT, D-limited mice failed to produce a DEX response.

151 d B-1a cell IgM developing in the absence of TdT, increased in 7- to 24-mo-old mice as compared with

152 In the absence of TdT, the pseudo-VH gene segment present in the minilocus

153 antial N-addition despite initial absence of TdT.

154 minilocus mice in the presence or absence of TdT.

155 The action of TdT on mouse TCR genes accounts for approximately 90% of

156 Thus, the action of TdT produces an adult repertoire that is both different

157 ensive postneonatally due to the activity of TdT, which adds nontemplated N nucleotides to Ig and TCR

158 uclear localization or enzymatic activity of TdT.

159 The physical association of TdT with Ku suggests a possible mechanism by which TdT i

160 As coexpression of TdT and PAX5 under physiologic circumstances is restrict

161 Complementation of TdT expression in TdT(-/-) mice by early forced expressi

162 tural alterations, 2) enzymatic detection of TdT-positive DNA degradation, and 3) automated cytometri

163 The second helix-hairpin-helix domain of TdT, but not the first, is required for activity.

164 Ikaros to the upstream regulatory element of TdT.

165 ntial for 1) up-regulating the expression of TdT and IL-7R alpha, 2) initiating the production of cmu

166 Expression of TdT and the lymphoid-associated genes Ig beta and RAG-1,

167 Further, expression of TdT, a molecule that is normally down-regulated by a fun

168 e N-nucleotide addition by the short form of TdT, but did not increase nucleotide deletion from codin

169 activity and function of the long isoform of TdT (TdTL) have not been determined.

170 The long isoform of TdT was found to reduce N-nucleotide addition by the sho

171 c mutation are compensated by early onset of TdT activity and other mechanisms that contribute to CDR

172 % CD19(+) B cells with normal percentages of TdT(+)VpreB(+)CD19(-) B cell precursors.

173 demonstrates that the N-terminal portion of TdT, including the BRCA-1 C-terminal (BRCT) domain, is n

174 cell lines results in a marked reduction of TdT expression.

175 riments reveal that the N-terminal region of TdT (131 amino acids) is essential for interaction with

176 ggested that the entire C-terminal region of TdT is necessary for N-nucleotide addition in vivo.

177 eported structure of the catalytic region of TdT.

178 Consequently, up-regulation of TdT and IL-7R alpha expression requires signaling throug

179 chains, together with the down-regulation of TdT transcription in pre-B cells (prior to light chain p

180 production results in the down-regulation of TdT.

181 cin results in transcriptional repression of TdT expression.

182 in TdT-deficient BM largely matched that of TdT-sufficient adult cells.

183 ed expression of the short splice variant of TdT-restored WT proportions of J558 Id+ clones and also

184 , we examined the effect of mu production on TdT gene expression in B lineage subsets from normal mic

185 ion of pre-pro-B cells (B220+, HSA-, TdT- or TdT+, c[mu-]) from adult rat, mouse, and human BM.

186 nd P berghei iRBCs with apoptotic parasites (TdT(+)) exhibited minimal platelet binding (<5%), which

187 In particular, TdT-mediated nucleotide insertions varied depending on a

188 hoid (B220(+)CD19(+)CD43(+)sIgM(-), PAX5(+), TdT(+), IgH rearranged)/myeloid (CD11b/Mac1(+), c-fms(+)

189 these differences reflect limited perinatal TdT activity versus differences in the fetal/adult envir

190 lt of the action of a unique DNA polymerase, TdT.

191 and PAX-5, and were uniformly TdT-positive (TdT+).

192 Such regulated access would help to prevent TdT from acting at other types of broken ends and degrad

193 Although purified TdT can act at free DNA ends, its ability to add nucleot

194 on-chip labeling method, we also quantified TdT mediated signal amplification on the surface by immo

195 e of Ku80, indicating that Ku80 may regulate TdT's activity at DNA ends in vivo.

196 chain (mu) protein might negatively regulate TdT expression.

197 of Ikaros as well as its ability to regulate TdT expression during thymocyte differentiation.

198 nd the failure of pro-B cells to up-regulate TdT and the IL-7R alpha (but not the common gamma-chain)

199 consisting of 35.4% and 7.4%, respectively, TdT+ cells, generated B-lineage cells in overnight cultu

200 were similar to Lin- c-Kit(high) L-selectin+ TdT+ RAG-1- progenitors present in the marrow, suggestin

201 ) (DQ52, DSP, or DST) gene segment sequence, TdT activity, or both to produce D99, all three D-limite

202 monstrated by morphological changes, in situ TdT labeling and internucleosomal laddering.

203 osis-associated DNA strand breaks by in situ TdT labeling showed that a significant number of flavopi

204 The in situ TdT-mediated biotin-dUTP nick end labeling (TUNEL) metho

205 tor for occupancy of the lymphocyte-specific TdT promoter.

206 ed for degeneration-induced silver staining, TdT-mediated dUTP-digoxigenin nick end-labeling (TUNEL)

207 Using tetramer staining, TdT(-/-) and TdT(+/+) NOD mice showed similar frequencie

208 [DEX]) by challenging adult TdT-sufficient (TdT(+/+)) and TdT-deficient (TdT(-/-)) gene-targeted mic

209 ndolyl diketo acids that specifically target TdT and behave as nucleotide-competitive inhibitors.

210 The TUNEL technique (TdT-mediated dUTP nick-end labeling) was used to detect

211 These findings demonstrate that TdT plays a critical role in the magnitude and breadth o

212 In this report, we establish that TdT forms a stable complex with DNA-PK.

213 n addition to the G preference, we find that TdT adds strings of purines or strings of pyrimidines at

214 The current model is that TdT adds N regions during V(D)J recombination by random

215 Based on these data, we propose that TdT does not add to recombination junctions through rand

216 orded to the newborn, may be the reason that TdT expression is delayed in ontogeny.

217 th a baculovirus expression system show that TdT can interact specifically with each of the Ku subuni

218 These data show that TdT is essential for the generation of the predominant h

219 blasts, sorted postimmunization, showed that TdT(-/-) mice generate a lower frequency of the predomin

220 These data suggest that TdT expression is initiated as c-kit downregulation begi

221 those in late term fetuses, suggesting that TdT is fully active at the onset of VDJ rearrangement; 2

222 Apoptotic cell death was evaluated by the TdT-mediated digoxigenin-dUTP nick-end labeling TUNEL as

223 the RNA by PaP acts as the initiator for the TdT-catalyzed polymerization of longer DNA strands from

224 hough the patterns of V(H)DJ(H) usage in the TdT-deficient B lineage cells paralleled that of wild-ty

225 cific for MCMV or for T cells or used in the TdT-dUTP terminal nick-end labeling (TUNEL) assay to det

226 e first expressed by late pre-B cells in the TdT-insufficient perinatal liver.

227 The combination of the TdT assay and confocal microscopy demonstrated that 20 m

228 hymocytes, Ikaros acts as a repressor of the TdT gene.

229 ed using hematoxylin and eosin staining, the TdT-dUTP terminal nick-end labeling (TUNEL) assay, and T

230 eractions between Ikaros dimers bound to the TdT promoter and those bound to pericentromeric repeat s

231 ed DNA binding affinity of Ikaros toward the TdT promoter.

232 Cell death was assayed using the TdT-dUTP terminal nick-end labeling (TUNEL) assay.

233 DNA fragmentation was determined using the TdT-dUTP terminal nick-end labeling assay and by agarose

234 in situ apoptosis detection assay using the TdT-mediated dUTP nick end labeling method.

235 distinctive in vivo role correlates with the TdT-like ability of pol mu, but not pol lambda, to act w

236 ere detected and the majority (93%) of these TdT-labeled neurons lacked evidence of tangle formation.

237 ditions led us to evaluate the role of these TdTs in the acquisition of nutrients other than iron.

238 Thus, TdT deficiency ameliorates the severity of disease in bo

239 traordinary cases of FL, which progressed to TdT(+)CD20(-) precursor B-lymphoblastic lymphoma (B-LBL)

240 peting terminal deoxynucleotide transferase (TdT) activity unlike any other B-family DNA polymerase.

241 were terminal deoxynucleotidyl transferase (TdT) -mediated deoxyuridine triphosphate (dUTP)-rhodamin

242 nzyme terminal deoxynucleotidyl transferase (TdT) adds nontemplate-derived nucleotides (N regions) to

243 press terminal deoxynucleotidyl transferase (TdT) and for other reasons, they are likely to express a

244 f the terminal deoxynucleotidyl transferase (TdT) and the paired box gene 5 (PAX 5) has been consiste

245 Terminal deoxynucleotidyl transferase (TdT) catalyzes the addition of nucleotides at the juncti

246 et of terminal deoxynucleotidyl transferase (TdT) expression in ontogeny.

247 e for terminal deoxynucleotidyl transferase (TdT) have nucleotide insertions at S-S junctions, indica

248 erase terminal deoxynucleotidyl transferase (TdT) in order to assess occupancy of the reaction interm

249 , and terminal deoxynucleotidyl transferase (TdT) in particular, have been widely used in enzymatic l

250 on by terminal deoxynucleotidyl transferase (TdT) increase the diversity of antigen receptors.

251 y the terminal deoxynucleotidyl transferase (TdT) reaction.

252 wn by terminal deoxynucleotidyl transferase (TdT) staining, and upregulation of wt p53 expression, as

253 ed by terminal deoxynucleotidyl transferase (TdT) to catalyze the sequential addition of a mixture of

254 Lo/-) terminal deoxynucleotidyl transferase (TdT)(+) cells in murine bone marrow are functional lymph

255 adult terminal deoxynucleotidyl transferase (TdT)(+/+) and TdT(-/-) donor cells, demonstrate preferen

256 Terminal deoxynucleotidyl transferase (TdT), a polymerase that adds random nucleotides (N regio

257 using terminal deoxynucleotidyl transferase (TdT), a template-independent DNA polymerase that catalyz

258 essed terminal deoxynucleotidyl transferase (TdT), the ETS transcription factor Spi-B, the nuclear fa

259 on of terminal deoxynucleotidyl transferase (TdT), which is normally expressed only in B cell precurs

260 ut by terminal deoxynucleotidyl transferase (TdT), whose only known physiological role is to create d

261 py of terminal deoxynucleotidyl transferase (TdT)-labeled lens slices confirmed that DNA fragmentatio

262 The terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin ni

263 Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate-biotin nick end

264 is in terminal deoxynucleotidyl transferase (TdT)-mediated dUTP biotin nick-end labeling assays.

265 h the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay and a

266 ed by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling and caspase-3 stain

267 using Terminal deoxynucleotidyl Transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) and DNA lad

268 with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) and flow cy

269 situ terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) to describe

270 ge of terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL)-positive le

271 UNEL (terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling) assays demo

272 Many terminal deoxynucleotidyl transferase (TdT)-positive neurons were detected and the majority (93

273 ) and terminal deoxynucleotidyl transferase (TdT).

274 P) by terminal deoxynucleotidyl transferase (TdT).

275 Terminal deoxynucleotidyl transferase (TdT; EC 2.7.7.31) adds nucleotides to DNA ends generated

276 Terminal deoxynucletidyl transferase (TdT) is overexpressed in some cancer types, where it mig

277 e terminal deoxyribonucleotidyl transferase (TdT)-mediated dUTP Nick End Labeling (TUNEL) assay.

278 Terminal transferase (TdT), when incubated with a purified(32)P-5"-end-labeled

279 Terminal transferase (TdT)-mediated deoxyuridine triphosphate (d-UTP)-biotin n

280 kers (terminal deoxynucleotidyl transferase [TdT], CD34; P <.10).

281 Terminal deoxynucleotidyl transferase [TdT]-mediated dUTP nick end labelling analysis demonstra

282 y production of TonB-dependent transporters (TdTs)-outer membrane proteins that facilitate nutrient t

283 outer membrane TonB-dependent transporters (TdTs.) Survival within host epithelial cells is importan

284 cytic cells, as determined by in vivo TUNEL (TdT-mediated dUTP nick-end labeling) staining.

285 These findings suggest that the two TdT isoforms may act in concert to preserve the integrit

286 roles of the remaining four uncharacterized TdTs (TdfF, TdfG, TdfH, and TdfJ) remain elusive.

287 g beta, RAG-1, and PAX-5, and were uniformly TdT-positive (TdT+).

288 However, unlike TdT, synthesis by pol mu in this context is primarily in

289 , in situ staining for apoptotic cells using TdT-mediated dUTP-digoxigenin nick-end labeling (TUNEL),

290 With the goal of using TdT as an on-chip labeling method, we also quantified Td

291 hese mice during a stage in development when TdT is not expressed.

292 of Ag receptor genes, the mechanism by which TdT acts remains unclear.

293 th Ku suggests a possible mechanism by which TdT is recruited to the sites of DSBs such as V(D)J reco

294 appearance of discrete nuclear foci in which TdT and Ku colocalize.

295 nd studies of their substrate abilities with TdT.

296 ors was determined by cocrystallization with TdT, explaining why these compounds are competitive inhi

297 exhibit junctional diversity consistent with TdT-mediated N-addition, and TdT RNA is expressed exclus

298 Dual labeling with TdT and an antibody against protein disulfide isomerase,

299 l death was analyzed by double labeling with TdT-dUTP terminal nick-end labeling and cone-specific an

300 Partial overlap with TdT(+) cells suggested that distinctive early lymphocyte