ライフサイエンスコーパス: Thermotoga

1 cina, Pseudomonas, Bartonella, Nitrosomonas, Thermotoga, and Aquifex showed a strong preference for L

2 a role in several lineages, such as Aquifex, Thermotoga, and Fusobacterium.

3 oplasmatales and M. thermoautotrophicus with Thermotoga, and Halobacteriales with Actinobacteria, sug

4 Studies of the Escherichia, Neisseria, Thermotoga, and Mycobacteria clustered regularly intersp

5 proteins identifies Aquifex as grouping with Thermotoga another bacterial hyperthemophile belonging t

6 uctural differences were observed instead in Thermotoga compared to Thermoplasmatales and M. thermoau

7 es of the bacterial phylogenetic tree, i.e., Thermotoga, Deinococcus-Thermus, Cyanobacteria, spiroche

8 g DNA, a property that distinguishes it from Thermotoga endonuclease V.

9 The copper transport ATPase from Thermotoga maritima (CopA) provides a useful system for

10 Crystals of DAHPS from Thermotoga maritima (DAHPS(Tm)) were grown in the presen

11 of peptide chain release factor 1 (RF1) from Thermotoga maritima (gi 4981173) at 2.65 Angstrom resolu

12 tructure of a type III PanK, the enzyme from Thermotoga maritima (PanK(Tm)), solved at 2.0-A resoluti

13 crystal structures of complexes between the Thermotoga maritima (Tm) NadA K219R/Y107F variant and (i

14 tm0322) from the hyperthermophilic bacterium Thermotoga maritima (TM0322).

15 Mechanistic studies of a FDTS from Thermotoga maritima (TM0449) are presented here.

16 ed three-dimensional structure of GK-II from Thermotoga maritima (TM1585; PDB code 2b8n) revealed a n

17 Thermotoga maritima (Tma) EndoV recognizes and primarily

18 rofolate reductase from the hyperthermophile Thermotoga maritima (TmDHFR) are presented.

19 rofolate reductase from the hyperthermophile Thermotoga maritima (TmDHFR) has been examined by enzyme

20 R) and the dimeric, thermophilic enzyme from Thermotoga maritima (TmDHFR).

21 al analysis of GH36 alpha-galactosidase from Thermotoga maritima (TmGalA).

22 N-utilizing substance A protein (NusA) from Thermotoga maritima (TmNusA), a protein involved in tran

23 of the GDP complex of the YjeQ protein from Thermotoga maritima (TmYjeQ), a member of the YjeQ GTPas

24 me bacteria, including the hyperthermophiles Thermotoga maritima and Aquifex aeolicus.

25 Co(2+)-requiring alkaline phosphatases from Thermotoga maritima and Bacillus subtilis have a His and

26 Alkaline phosphatases from organisms such as Thermotoga maritima and Bacillus subtilis require cobalt

27 ochemical properties of a TF:S7 complex from Thermotoga maritima and determined its crystal structure

28 We have created a series of Thermotoga maritima and Escherichia coli pseudouridine 5

29 We expressed and purified MpgII from Thermotoga maritima and found that the enzyme releases b

30 d the crystal structure of the PanK-III from Thermotoga maritima and identified it as a member of the

31 Co-cultivation of the hyperthermophiles Thermotoga maritima and Methanococcus jannaschii resulte

32 eus, and two from the Gram-negative bacteria Thermotoga maritima and Pseudomonas aeruginosa.

33 have characterized the ECF transporters from Thermotoga maritima and Streptococcus thermophilus.

34 of HU from the hyperthermophilic eubacterium Thermotoga maritima are shown here to differ significant

35 rate-binding domains of HemK from E.coli and Thermotoga maritima are structurally similar, despite th

36 Using the hydrogenase maturase HydE from Thermotoga maritima as a template, we obtained several u

37 ion crystal structure of lysine bound to the Thermotoga maritima asd lysine riboswitch ligand-binding

38 ucible transcriptional repressor, HrcA, from Thermotoga maritima at 2.2A resolution.

39 of the PLP synthase complex (YaaD-YaaE) from Thermotoga maritima at 2.9 A resolution.

40 structure of the FliY catalytic domain from Thermotoga maritima bears strong resemblance to the midd

41 The open reading frame TM1643 of Thermotoga maritima belongs to a large family of protein

42 In Thermotoga maritima both candidate genes (in an original

43 ed to map conformational states of CorA from Thermotoga maritima by determining which residues suppor

44 -examined the completely sequenced genome of Thermotoga maritima by employing the combined use of the

45 haracterize the CheA-receptor interaction in Thermotoga maritima by NMR spectroscopy and validate the

46 We show here that the Cmr complex from Thermotoga maritima can cleave an ssRNA target that is c

47 owever, we show that both RimO and MiaB from Thermotoga maritima catalyze methyl transfer from SAM to

48 previously isolated by random sequencing of Thermotoga maritima cDNA clones.

49 The 2.6 A resolution crystal structure of Thermotoga maritima CheA (290-671) histidine kinase reve

50 cture of a soluble ternary complex formed by Thermotoga maritima CheA (TmCheA), CheW, and receptor si

51 investigate the two ATP-binding sites of the Thermotoga maritima CheA dimer (TmCheA) and the single s

52 The structure of Thermotoga maritima CheA domain P2 in complex with CheY

53 of nucleotide binding to the active site of Thermotoga maritima CheA was investigated using stopped-

54 ity of the phospho-accepting His (His-45) in Thermotoga maritima CheA.

55 thylation analyses utilizing S. enterica and Thermotoga maritima CheR proteins and MCPs indicate that

56 sed on a crystal structure of the homologous Thermotoga maritima class III RNR, showing its architect

57 rystal structures of a fragment of MetH from Thermotoga maritima comprising the domains that bind Hcy

58 ities, while the hyperthermophilic bacterium Thermotoga maritima contains only one, pyruvate ferredox

59 Thermotoga maritima CorA (TmCorA) is the only member of

60 The crystal structures of Thermotoga maritima CorA provide an excellent structural

61 The crystal structure of Thermotoga maritima CorA shows a homopentamer with two t

62 eolicus GyrA/ParC CTD with the GyrA CTD from Thermotoga maritima creates an enzyme that negatively su

63 scattering studies of thermostable CheA from Thermotoga maritima determine that the His-containing su

64 hile those of Mycobacterium tuberculosis and Thermotoga maritima did not.

65 ymerase derived from Thermus species Z05 and Thermotoga maritima DNA polymerases.

66 ed the 1.8-A resolution crystal structure of Thermotoga maritima DrrB, providing a second structure o

67 The gene buk2 from Thermotoga maritima encodes a member of the ASKHA (aceta

68 nserved residues D43, E89, D110, and H214 in Thermotoga maritima endonuclease V catalysis.

69 was performed in nine conserved positions of Thermotoga maritima endonuclease V to identify amino aci

70 t seven conserved motifs of the thermostable Thermotoga maritima endonuclease V to probe for residues

71 Thermotoga maritima exo-beta-fructosidase (BfrA) secrete

72 , we showed that FMN-free diferrous FDP from Thermotoga maritima exposed to 1 equiv NO forms a stable

73 ors with a single enzyme 'model system', the Thermotoga maritima family 1 beta-glucosidase, TmGH1.

74 he hyperthermophilic and anaerobic bacterium Thermotoga maritima ferments a wide variety of carbohydr

75 interaction between the N-terminal domain of Thermotoga maritima FliG (FliG(N)) and peptides correspo

76 Furthermore, we show that Thermotoga maritima FliM and FliN form a 1:3 complex str

77 e (6PGDH) from a hyperthermophilic bacterium Thermotoga maritima from its natural coenzyme NADP(+) to

78 The crystal structures of Thermotoga maritima GTase and its complex with the inhib

79 lease V from the hyperthermophilic bacterium Thermotoga maritima has been cloned and expressed in Esc

80 Thermotoga maritima HpkA is a transmembrane histidine ki

81 at HU from the hyperthermophilic eubacterium Thermotoga maritima HU bends DNA and constrains negative

82 presents a 2.3-kb locus with similarity to a Thermotoga maritima hypothetical protein, while another

83 report three crystal structures of ThiI from Thermotoga maritima in complex with a truncated tRNA.

84 ion of a sensor HK, one from the thermophile Thermotoga maritima in complex with ADPbetaN at 1.9 A re

85 heterologously produced the NfnAB complex of Thermotoga maritima in Escherichia coli, provided kineti

86 ve solved the crystal structure of FtsA from Thermotoga maritima in the apo and ATP-bound form.

87 ed five different structures of FGAR-AT from Thermotoga maritima in the presence of substrates, a sub

88 production of the GH10 xylanase Xyl10B from Thermotoga maritima in transplastomic plants and demonst

89 Thermotoga maritima is a marine hyperthermophilic microo

90 work, a structure of the PurLQS complex from Thermotoga maritima is described revealing a 2:1:1 stoic

91 Structural characterization of Thermotoga maritima IscU by CD and high resolution NMR y

92 of the 174-nucleotide sensing domain of the Thermotoga maritima lysine riboswitch in the lysine-boun

93 Here we report that Thermotoga maritima MazG protein (Tm-MazG), the product

94 pe and C150/154/157A triple variant forms of Thermotoga maritima MiaB have revealed the presence of t

95 dy the assembly and mechanical properties of Thermotoga maritima MreB in the presence of different nu

96 Here, we studied the assembly of Thermotoga maritima MreB triggered by ATP in vitro and c

97 A whole-genome alignment of Thermotoga maritima MSB8 and Thermotoga neapolitana NS-E

98 The 1,860,725-base-pair genome of Thermotoga maritima MSB8 contains 1,877 predicted coding

99 sequence of the hyperthermophilic bacterium Thermotoga maritima MSB8 presents evidence for lateral g

100 The hyperthermophilic bacterium Thermotoga maritima MSB8 was grown on a variety of carbo

101 Molecular replacement with the Thermotoga maritima NifS model was used to determine pha

102 ng growth of the hyperthermophilic bacterium Thermotoga maritima on 14 monosaccharide and polysacchar

103 The extremophile Thermotoga maritima possesses a remarkable array of carb

104 The hyperthermophilic eubacterium Thermotoga maritima possesses an operon encoding an Hsp7

105 Endonuclease V obtained from Thermotoga maritima preferentially cleaves purine mismat

106 n success, DXMS analysis was attempted on 24 Thermotoga maritima proteins with varying crystallizatio

107 C-terminal amphipathic helix in vitro using Thermotoga maritima proteins.

108 Here, we report the analysis of the Thermotoga maritima proteome, in which we compare the pr

109 The crystal structures of Thermotoga maritima Psi55S, and its complex with RNA, ha

110 cture of this C-terminal domain of FliN from Thermotoga maritima revealed a saddle-shaped dimer forme

111 ure of the FliM middle domain (FliM(M)) from Thermotoga maritima reveals a pseudo-2-fold symmetric to

112 main (P1) from the chemotaxis kinase CheA of Thermotoga maritima reveals a remarkable degree of struc

113 at 3.85 A resolution of the RNA component of Thermotoga maritima ribonuclease P.

114 es (F17 and R89) are essential for efficient Thermotoga maritima RNase P activity.

115 We report the crystal structure of the Thermotoga maritima RNase P holoenzyme in complex with t

116 at the cytoplasmic helix-turn-helix motif of Thermotoga maritima RodZ directly interacts with monomer

117 The 1.6 A crystal structure of Thermotoga maritima RuvB together with five mutant struc

118 Here, we report a crystal structure of Thermotoga maritima SecA at 1.9 A resolution.

119 The recent crystal structure of the Thermotoga maritima SecA-SecYEG complex shows the ATPase

120 ur data support the in vivo relevance of the Thermotoga maritima SecA.SecYEG crystal structure that v

121 iety of contexts, including the structure of Thermotoga maritima sigmaA region 4 described herein.

122 itor binding to potently inhibited Sirt1 and Thermotoga maritima Sir2 and to moderately inhibited Sir

123 of the N and C-terminal globular domains of Thermotoga maritima SMC in Escherichia coli by replacing

124 The 2.17 A resolution crystal structure of a Thermotoga maritima soluble receptor (Tm14) reveals dist

125 occasionally found also in bacteria such as Thermotoga maritima that do not utilise a PEP-PTS system

126 f loop 2, which are analogous to residues in Thermotoga maritima that mediate cross-talk.

127 we report the structures of hTK1 and of the Thermotoga maritima thymidine kinase (TmTK) in complex w

128 The Thermotoga maritima TM0065 gene codes for a protein (TM-

129 o be a potent inhibitor (Ki = 8.2 nM) of the Thermotoga maritima TmGH1 beta-glucosidase.

130 olution to TrpB from Pyrococcus furiosus and Thermotoga maritima to generate a suite of catalysts for

131 The gene for the Thermotoga maritima Trk potassium transporter component

132 TruB apo enzyme, as well as the structure of Thermotoga maritima TruB in complex with RNA.

133 RNase III of the hyperthermophilic bacterium Thermotoga maritima was analyzed using purified recombin

134 synthase from the thermophilic microorganism Thermotoga maritima was cloned, and the enzyme was overe

135 protein from the hyperthermophilic bacterium Thermotoga maritima was determined at 1.2-A resolution b

136 Ribosome recycling factor (RRF) of Thermotoga maritima was expressed in Escherichia coli fr

137 Previously, Tm0936 from Thermotoga maritima was shown to catalyze the deaminatio

138 NagA from Thermotoga maritima was shown to require a single divale

139 mophilic eubacteria Thermus thermophilus and Thermotoga maritima were cloned, sequenced, and expresse

140 ated interactions between FliM and FliG from Thermotoga maritima with X-ray crystallography and pulse

141 Thermotoga maritima XseA/B homologs TM1768 and TM1769 we

142 Bacillus subtilis, Sulfolobus tokodaii, and Thermotoga maritima) and two eukaryotic (Saccharomyces c

143 chaeoglobus fulgidus, and from the bacterium Thermotoga maritima) into the E. coli expression vector

144 les but later recolonized a hot environment (Thermotoga maritima) relied in their evolutionary strate

145 M0487 (a 102-residue alpha+beta protein from Thermotoga maritima), we predicted the complete, topolog

146 in vitro methylation of chemoreceptors from Thermotoga maritima, a hyperthermophile that has served

147 prokaryotes and eukaryotes, was cloned from Thermotoga maritima, a hyperthermophilic bacterium.

148 o acid sequence level to the enzyme found in Thermotoga maritima, a thermophilic eubacteria, and sugg

149 a-glycosidases from Sulfolobus solfataricus, Thermotoga maritima, and Caldocellum saccharolyticum.

150 P superfamilies against the entire genome of Thermotoga maritima, and make over a 100 new fold predic

151 ic organism, the hyperthermophilic bacterium Thermotoga maritima, and those of close homologs from me

152 r to the KH1 domain of the NusA protein from Thermotoga maritima, another cold-shock associated RNA-b

153 9 family proteins from a variety of sources (Thermotoga maritima, Bacillus subtilis, Acinetobacter ba

154 f an enzyme of unknown activity, Tm0936 from Thermotoga maritima, by docking high-energy intermediate

155 glucose-6-phosphate dehydrogenase (Gpd) from Thermotoga maritima, demonstrated robust activity over a

156 arrel domain from the thermophilic bacteria, Thermotoga maritima, enabled an NMR-based site-specific

157 te of CspTm, a small cold-shock protein from Thermotoga maritima, engineered to contain a single tryp

158 -isopentenyladenosine of tRNA in E. coli and Thermotoga maritima, has been demonstrated to harbor two

159 HK853 and its response regulator RR468 from Thermotoga maritima, here we report a pH-mediated confor

160 hemical studies demonstrate that TM1635 from Thermotoga maritima, originally annotated as a putative

161 Surprisingly, even Thermotoga maritima, previously considered to have only

162 ith Lactobacillus casei, and 23 and 40% with Thermotoga maritima, respectively.

163 Unlike endonuclease V from Thermotoga maritima, Salmonella endonucleae V can only t

164 sophile Escherichia coli and the thermophile Thermotoga maritima, subunit dissociation activates at t

165 nate lyase from the thermophilic eubacterium Thermotoga maritima, the archaebacterial lyase contains

166 he genome of the hyperthermophilic bacterium Thermotoga maritima, TM0504 encodes a putative signaling

167 otein from the hyperthermostable eubacterium Thermotoga maritima, TmHU as an efficient gene transfer

168 ding by the EcfS subunit for riboflavin from Thermotoga maritima, TmRibU.

169 In Thermotoga maritima, two PhoU homologues have been ident

170 domains from a hyperthermophilic bacterium, Thermotoga maritima, was cloned and expressed in Escheri

171 it of RNase P from a thermophilic bacterium, Thermotoga maritima, was overexpressed in and purified f

172 e conserved hypothetical protein TM0979 from Thermotoga maritima, we demonstrate the capabilities of

173 rototyping it using the simple microorganism Thermotoga maritima, we show our model accurately simula

174 atabolite-linked transcriptional networks in Thermotoga maritima, we used full-genome DNA microarray

175 he heterodimeric ABC exporter TM287/288 from Thermotoga maritima, which contains a non-canonical ATP

176 f a larger fragment of the FliG protein from Thermotoga maritima, which encompasses the middle and C-

177 omes of several unusual organisms, including Thermotoga maritima, whose genome reveals extensive pote

178 ion of P1 and P3P4 from the hyperthermophile Thermotoga maritima.

179 UvrB from Bacillus caldotenax and UvrC from Thermotoga maritima.

180 tal structure of most of the FliN protein of Thermotoga maritima.

181 sequence of the hyperthermophilic bacterium Thermotoga maritima.

182 structure of the C-terminal 70% of FliG from Thermotoga maritima.

183 d loop of the group I NifS-like protein from Thermotoga maritima.

184 ay in the marine hyperthermophilic bacterium Thermotoga maritima.

185 opic labeling of a sigma70-like subunit from Thermotoga maritima.

186 o the proteome of the thermophilic bacterium Thermotoga maritima.

187 genome of Mycoplasma genitalium, and 23% in Thermotoga maritima.

188 the crystal structure to 2.2 A of MinC from Thermotoga maritima.

189 ecombinant proteins from the model bacterium Thermotoga maritima.

190 n was detected in the thermophilic bacterium Thermotoga maritima.

191 minators of this type, with the exception of Thermotoga maritima.

192 he genome of the hyperthermophilic bacterium Thermotoga maritima.

193 liG-C from the hyperthermophilic eubacterium Thermotoga maritima.

194 Here, we describe a UDG from the thermophile Thermotoga maritima.

195 in ThyX from the hyperthermophilic bacterium Thermotoga maritima.

196 sequences in the hyperthermophilic bacterium Thermotoga maritima.

197 gulator from the hyperthermophilic bacterium Thermotoga maritima.

198 protein in the hydrogen-producing bacterium Thermotoga maritima.

199 raction modes of chemoreceptor and CheW from Thermotoga maritima.

200 e sugar kinome in the thermophilic bacterium Thermotoga maritima.

201 otoga sp. strain RQ2 is probably a strain of Thermotoga maritima.

202 eW and the P4-P5 fragment of CheA, both from Thermotoga maritima.

203 exonuclease activity in endonuclease V from Thermotoga maritima.

204 amined a deflavinated FDP (deflavo-FDP) from Thermotoga maritima.

205 catalysis by DHFR from the hyperthermophile Thermotoga maritima.

206 e central metabolic network of the bacterium Thermotoga maritima.

207 5 angstrom (A), obtained for components from Thermotoga maritima.

208 we have characterized an ExoVII homolog from Thermotoga maritima.

209 ichia coli, and purified untagged MreB1 from Thermotoga maritima.

210 This operon is also found in Thermotoga naphthophila strain RKU-10 but no other Therm

211 Thermotoga neapolitana (Tne) DNA polymerase belongs to t

212 Thermotoga neapolitana 1,4-beta-d-glucan glucohydrolase

213 Construction of a Thermotoga neapolitana adenylate kinase (AK) library usi

214 acillus subtilis adenylate kinase (BsAK) and Thermotoga neapolitana adenylate kinase (TnAK) with iden

215 Backbone conformational dynamics of Thermotoga neapolitana adenylate kinase in the free form

216 me alignment of Thermotoga maritima MSB8 and Thermotoga neapolitana NS-E has revealed numerous large-

217 Characterization in Thermotoga neapolitana of a catabolic gene cluster encod

218 1,4-beta-D-glucan glucohydrolase (GghA) from Thermotoga neapolitana.

219 thermostable two-domain endo-acting ABN from Thermotoga petrophila (TpABN) revealed how some GH43 ABN

220 report on the ligand-bound structure of the Thermotoga petrophila fluoride riboswitch, which adopts

221 Thermotoga sp. RQ2 differs from T. maritima in its genes

222 Thermotoga sp. strain RQ2 is probably a strain of Thermo

223 extent and consequences of gene flow within Thermotoga species and strains.

224 The hyperthermophilic bacterium Thermotoga species strain RQ7 harbors an 846-bp plasmid,

225 e primary enzyme for attacking cellobiose in Thermotoga spp.

226 dization study was initiated to compare nine Thermotoga strains to the sequenced T. maritima MSB8.

227 .L1917 was acquired from other bacteria like Thermotoga subterranea and Cbu.L1951 from lower eukaryot

228 P motif; V substitutes for R only in HU from Thermotoga, Thermus and Deinococcus.

229 phosphate suggests that the novel pathway in Thermotoga utilizes a phosphorylated derivative of inosi