ライフサイエンスコーパス: Ti plasmid

1 system which involves the virB operon of the Ti plasmid.

2 raM, an 11 kDa protein also coded for by the Ti plasmid.

3 t are required for conjugal transfer of this Ti plasmid.

4 thought to be absent from the nopaline-type Ti plasmid.

5 mologues is oriented counterclockwise on the Ti plasmid.

6 self-transmissible but is mobilizable by the Ti plasmid.

7 brane independently of other products of the Ti plasmid.

8 c sensing determinant is associated with the Ti plasmid.

9 but distinct from, a homolog located on the Ti plasmid.

10 e form of a 25-kb T-DNA sector of a resident Ti plasmid.

11 group are localized in the occ region of the Ti plasmid.

12 containing an octopine-type or nopaline-type Ti plasmid.

13 r regulon including the repABC operon on the Ti plasmid.

14 was identified, in addition to a copy on the Ti plasmid.

15 g regulatory proteins are encoded within the Ti plasmid.

16 B-repC intergenic region of an octopine-type Ti plasmid.

17 scription of at least seven promoters on the Ti plasmid.

18 pD) in the repABC operon of an octopine-type Ti plasmid.

19 ulates the octopine catabolism operon of the Ti plasmid.

20 enhances the copy number of a nopaline-type Ti plasmid.

21 eplication, stability and copy number of the Ti plasmid.

22 erted into the T-DNA region of the bacterial Ti plasmid.

23 is expressed from its native promoter on the Ti plasmid.

24 ng virulence-associated VirB proteins of the Ti plasmid.

25 ulates the octopine catabolism operon of the Ti plasmid.

26 virF-T-DNA intergenic regions of an octopine Ti plasmid.

27 D4 restored transfer to a traG mutant of the Ti plasmid.

28 1 insertions in the trb operon of full-sized Ti plasmids.

29 located in the T(R) region of octopine-type Ti plasmids.

30 ge reaction within the 25-bp right border of Ti plasmids.

31 sion of the traR genes on these two types of Ti plasmids.

32 ation and conjugation of the tumor-inducing (Ti) plasmid.

33 at target promoters of the tumour-inducing (Ti) plasmid.

34 nd conjugation genes of the tumour-inducing (Ti) plasmid.

35 genes required for conjugal transfer of the Ti plasmid and also enhances the copy number of a nopali

36 ulates the octopine catabolism operon of the Ti plasmid and is also an autorepressor.

37 ell as the donor strain, carries a wild-type Ti plasmid and is capable of vir gene expression.

38 operon was previously shown to reside on the Ti plasmid and to be directly inducible by octopine.

39 d the DNA sequences of these regions of both Ti plasmids and identified both conserved loci.

40 se signals, these strains block the entry of Ti plasmids and instead become efficient conjugal donors

41 al transfer of the Agrobacterium tumefaciens Ti plasmid are regulated by the quorum sensing transcrip

42 The products of several trb genes of the Ti plasmid are similar to those of other loci that encod

43 jugal transfer and T-DNA transfer systems of Ti plasmids, are believed to dictate specificity of the

44 In the mutant, traR was expressed from the Ti plasmid at a level about twofold lower than that in N

45 ne responsive repressor accR transferred the Ti plasmid at maximum frequencies at very low population

46 ed with the opines and AAI transferred their Ti plasmids at population levels about 10-fold lower tha

47 e genes (vir regulon) on the tumor-inducing (Ti) plasmid be induced by plant phenolic signals in an a

48 ns required for the conjugal transfer of the Ti plasmid between cells of Agrobacterium.

49 5, revealed that the 176-kb element is not a Ti plasmid but carries genes for catabolism of MOP, mann

50 s previously identified on the octopine-type Ti plasmid but thought to be absent from the nopaline-ty

51 jugation of the octopine-mannityl opine-type Ti plasmids by regulating the expression of traR via Occ

52 However, the quorum-dependent activation of Ti plasmid conjugal transfer exhibited a lag of almost 8

53 e population density-dependent regulation of Ti plasmid conjugal transfer.

54 te for TraR, the quorum-sensing activator of Ti plasmid conjugal transfer.

55 division, suggesting that while induction of Ti plasmid conjugation is an active process, the cells l

56 Likewise, Ti plasmid conjugation required TraA, TraF and TraG, and

57 g activator of the Agrobacterium tumefaciens Ti plasmid conjugation system, induces gene expression i

58 homoserine lactone-mediated autoinduction of Ti plasmid conjugative transfer by interacting directly

59 TraR-dependent elevation of Ti plasmid copy number caused a three- to fourfold incre

60 We conclude that Ti plasmid copy number is influenced by the quorum-sensi

61 g system resulted in an apparent increase in Ti plasmid copy number.

62 o enhanced rep gene expression and increased Ti plasmid copy number.

63 ysically separated from the remainder of the Ti-plasmid, creating a 'binary vector' system; this syst

64 The Ti plasmid did mobilize such plasmids if TraG(RP4) was e

65 However, the Ti plasmid did not mobilize transfer from an IncQ relaxo

66 r regions of octopine-type and nopaline-type Ti plasmids direct the transfer of oncogenic T-DNA from

67 of Agrobacterium tumefaciens tumor-inducing (Ti) plasmids direct the transfer of oncogenic portion of

68 cterium tumefaciens transfers a piece of its Ti plasmid DNA (transferred DNA or T-DNA) into plant cel

69 egration of a segment of its tumor-inducing (Ti) plasmid DNA into the genome of numerous plant specie

70 Since Ti plasmid donor cells both produce and respond to AAI,

71 tumefaciens depends on both chromosome- and Ti plasmid-encoded gene products.

72 The Ti plasmid-encoded vir genes of A. tumefaciens that are

73 y encoded sugar binding protein ChvE and the Ti plasmid-encoded VirA/VirG two-component regulatory sy

74 This transfer occurs in the absence of the Ti plasmid-encoded VirB and Trb systems.

75 terium required induction of tumor-inducing (Ti) plasmid-encoded virulence genes and growth at low te

76 d nopaline-type Ti plasmids, to identify all Ti-plasmid-encoded genes in the vir regulons of both pla

77 of RSF1010 and plasmid F, while genes of the Ti plasmid encoding other essential tra functions share

78 eotide sequence of an 8,755-bp region of the Ti plasmid encompassing the transposon insertions defini

79 he Agrobacterium tumefaciens tumor-inducing (Ti) plasmid enters infected plant cells and integrates i

80 mefaciens strains harboring an octopine-type Ti plasmid exhibit a similar activity which is not coded

81 nt for the vir regulon (or "virulon") of the Ti plasmid for the transfer of oncogenes from Agrobacter

82 ible for the conjugal transfer of the entire Ti plasmid from one bacterium to another.

83 Conjugal transfer of Ti plasmids from Agrobacterium spp. is controlled by a h

84 Conjugal transfer of the Ti plasmids from Agrobacterium tumefaciens is controlled

85 conjugative transfer of the tumor-inducing (Ti) plasmid from pathogenic strains to nonpathogenic der

86 copy number of traR or by disrupting traM, a Ti plasmid gene coding for an antiactivator specific for

87 caused a modest induction of virtually every Ti plasmid gene.

88 s in crown gall tumors are always matched by Ti plasmid genes conferring the ability to catabolize th

89 virB1 through virB11, and virD4 are the only Ti plasmid genes necessary for pilus assembly.

90 the culture supernatant did not require any Ti plasmid genes, except for VirE1, a specific chaperone

91 ium specific and can occur in the absence of Ti-plasmid genes.

92 Agrobacterium delivery of a Ti plasmid harboring both the ZFNs and a donor DNA const

93 t of the sex pilus encoded by IncP (RP4) and Ti plasmids has been identified as a circular pilin prot

94 igin, but that the opine signals for the two Ti plasmids have evolved divergently through changes in

95 nsfer systems encoded by the tumor-inducing (Ti) plasmid have been previously identified in Agrobacte

96 The Ti plasmid in Agrobacterium tumefaciens strain 15955 car

97 gene, traS, was previously found on the same Ti plasmid in an operon that directs the uptake of manno

98 nd conjugal transfer of the tumour-inducing (Ti) plasmid in the plant pathogen Agrobacterium tumefaci

99 egetative replication of the tumor-inducing (Ti) plasmid in the presence of the autoinducer 3-oxoocta

100 Subcloning of the Ti plasmid indicated that the virA locus determines whic

101 requires at least one tra gene found on its Ti plasmid, indicating that this element is not self-tra

102 the arc operons of pTiC58 and pTiChry5, two Ti plasmids inducible for transfer by agrocinopines A-B

103 By transferring different Ti plasmids into isogenic chromosomal backgrounds, we sh

104 A (transferred DNA) from its tumor-inducing (Ti) plasmid into dicotyledonous plants.

105 ers DNA from the resident 'tumour-inducing' (Ti) plasmid into plant cells, where it can be stably int

106 tumefaciens cells revealed that virA on the Ti plasmid is involved and that neither virB nor virD ge

107 njugal transfer of Agrobacterium tumefaciens Ti plasmids is controlled by a hierarchical system in wh

108 njugal transfer of Agrobacterium tumefaciens Ti plasmids is regulated by quorum sensing via the trans

109 njugal transfer of Agrobacterium tumefaciens Ti plasmids is regulated by quorum sensing via TraR and

110 um tumefaciens wide-host-range octopine-type Ti plasmids is regulated by the LuxR-type transcriptiona

111 Conjugative transfer of Agrobacterium Ti plasmids is regulated by TraR, a quorum-sensing activ

112 njugal transfer of Agrobacterium tumefaciens Ti plasmids is regulated by two hierarchical signalling

113 Expression of traR in octopine-type Ti plasmids is stimulated by OccR in response to octopin

114 ion of the repABC operon of tumour inducing (Ti) plasmids is both negatively autoregulated by the Rep

115 Donors harboring Ti plasmids lacking TraM were fully induced by the quorm

116 the Mpf genes of pTiC58, indicating that the Ti plasmid mating bridge can interact with the RP4 relax

117 ncQ but not the Ti plasmid relaxosome to the Ti plasmid mating bridge.

118 sfer system exhibited similar phenotypes for Ti plasmid-mediated mobilization of the IncQ vector.

119 This suggested that the copy number of the Ti plasmid might increase in response to AS, a hypothesi

120 M homologue called TraM2, not encoded on the Ti plasmid of A. tumefaciens A6, was identified, in addi

121 homology to octopine VirF is encoded by the Ti plasmid of the nopaline C58 strain of Agrobacterium.

122 The replicator regions of the Ti plasmids of Agrobacterium tumefaciens belong to the r

123 The Ti plasmids of Agrobacterium tumefaciens do not mobilize

124 Some or possibly all Ti plasmids of Agrobacterium tumefaciens encode a bicist

125 Here, we demonstrate that two Ti plasmids of Agrobacterium tumefaciens encode robust e

126 The Ti plasmids of Agrobacterium tumefaciens encode two tran

127 Conjugative transfer of the Ti plasmids of Agrobacterium tumefaciens is controlled b

128 Conjugal transfer of Ti plasmids of Agrobacterium tumefaciens is controlled b

129 Octopine-type Ti plasmids of Agrobacterium tumefaciens require the quo

130 Ti plasmids of Agrobacterium tumefaciens, in addition to

131 Tumour-inducing (Ti) plasmids of Agrobacterium tumefaciens replicate via

132 e rep and tra genes from the tumor-inducing (Ti) plasmids of Agrobacterium tumefaciens, including hom

133 e isopentenyltransferase gene (ipt) from the Ti-plasmid of Agrobacterium tumefaciens increases cytoki

134 erate the lower strand of the T-DNA from the Ti plasmid, producing single-stranded DNA molecules (T s

135 nd -I of the trb region of the octopine-type Ti plasmid pTi15955 and of the tra2 core region of RP4.

136 encoded by the octopine/mannityl opine-type Ti plasmid pTi15955 is related at the nucleotide sequenc

137 We have analysed a 20 kb region of the Ti plasmid pTi15955 that is required for the catabolism

138 ment 4 from the octopine/mannityl opine-type Ti plasmid pTi15955, grew well with agropine (AGR) but s

139 e, trlR, on the octopine-mannityl opine-type Ti plasmids pTi15955 and pTiR10.

140 s encoded by three Ti plasmids, the octopine Ti plasmid pTiA6NC, the supervirulent plasmid pTiBo542,

141 A, repB, and repC genes of the octopine-type Ti plasmid pTiB6S3 as well as to other repA, -B, and -C

142 r opines known to induce transfer of another Ti plasmid, pTiBo542.

143 Two regions of the nopaline-type Ti plasmid pTiC58 are important for conjugal transfer of

144 The acc locus from the Ti plasmid pTiC58 confers utilization of and chemotaxis

145 The replicator (rep) of the nopaline-type Ti plasmid pTiC58 is located adjacent to the trb operon

146 Conjugation of the Agrobacterium Ti plasmid pTiC58 is regulated by a hierarchy involving

147 Conjugal transfer of the Ti plasmid pTiC58 is regulated by a quorum-sensing syste

148 rvirulent plasmid pTiBo542, and the nopaline Ti plasmid pTiC58, are inner membrane proteins and that

149 o regions, tra and trb, of the nopaline-type Ti plasmid pTiC58.

150 of which are conserved in the nopaline-type Ti plasmid pTiC58.

151 ker-exchanged into the transfer-constitutive Ti plasmid pTiC58DeltaaccR mutations in trbB, -C, -D, -E

152 n this study, we demonstrated that the novel Ti plasmid pTiChry5 is induced to transfer at high frequ

153 ium tumefaciens, including homologues of the Ti plasmid quorum-sensing regulators TraI, TraR, and Tra

154 nd cells lacking TrbJ and TrbK are efficient Ti plasmid recipients.

155 (RP4) and VirD4 couples the IncQ but not the Ti plasmid relaxosome to the Ti plasmid mating bridge.

156 In the region upstream of the octopine-type Ti plasmid repABC operon, three promoters were recently

157 Conjugation of wide-host-range octopine-type Ti plasmids requires a tumor-released arginine derivativ

158 ation origin of an Agrobacterium tumefaciens Ti plasmid resides fully within its repC gene.

159 B proteins in a recipient strain lacking the Ti plasmid revealed that the VirB7 to VirB10 proteins yi

160 Octopine-type Ti plasmids such as pTi15955, pTiA6 and pTiR10 direct th

161 Among the Ti plasmid systems studied to date, only one of the two

162 The mechanisms that ensure that Ti plasmid T-DNA genes encoding proteins involved in the

163 s related to TraG of RP4 and to VirD4 of the Ti plasmid T-DNA transfer system.

164 ts, is essential for the transmission of the Ti-plasmid T-DNA from Agrobacterium tumefaciens to plant

165 nding site, the tra-box, located upstream of Ti plasmid target promoters.

166 the characterized tra and trb regions of the Ti plasmid, that conjugation does not require functions

167 strated that VirB3 proteins encoded by three Ti plasmids, the octopine Ti plasmid pTiA6NC, the superv

168 aciens transfers part of the tumor-inducing (Ti) plasmid, the T-DNA, to a plant cell where it eventua

169 s by transferring a portion of the bacterial Ti-plasmid, the T-DNA, to the plant and integrating the

170 loping tumors, wild-type C58 transferred the Ti plasmid to recipients, yielding transconjugants by 14

171 all genes of the octopine- and nopaline-type Ti plasmids, to identify all Ti-plasmid-encoded genes in

172 nds 3-oxo-C8-HSL and activates expression of Ti plasmid tra and rep genes, increasing conjugation and

173 The products of several Ti plasmid tra genes are related to those of other conju

174 tial for the TraR-mediated activation of the Ti plasmid Tra genes.

175 ate cell-density-dependent expression of the Ti plasmid tra regulon.

176 tem for maximal activity, and that while the Ti plasmid tra system recognizes diverse gram-negative b

177 cC has no biologically significant effect on Ti plasmid transfer or its regulatory system.

178 pigments, as well as Hrp protein secretion, Ti plasmid transfer, motility, biofilm formation, and ep

179 f the IncQ plasmid by pTiC58 did not inhibit Ti plasmid transfer, suggesting that the relaxosomes of

180 quencies similar to that at which the intact Ti plasmid transferred.

181 ncreases the copy number of an octopine-type Ti plasmid up to eightfold and that TraR activates trans

182 l induction with acetosyringone at pH 5.5 of Ti-plasmid vir genes.

183 Because the Ti plasmid virB gene products are hypothesized to form a

184 robacterium tumefaciens and induction of its Ti plasmid virulence (vir) genes by acetosyringone (AS).

185 ing in the transcriptional activation of the Ti plasmid virulence genes.

186 ts through activities of the tumor-inducing (Ti)-plasmid virulence (vir) genes of Agrobacterium tumef

187 opines A and B, the conjugal opines for this Ti plasmid, was detected only after the donors had reach

188 vealed that the VirB proteins encoded by the Ti plasmid were required.