ライフサイエンスコーパス: Tn

1 Tn complexes containing cTnT1 and cTnT4 (both fetal isof

2 Tn is composed of TnC, TnI, and TnT.

3 Tn syndrome is a rare autoimmune disease in which subpop

4 Tn-Seq is a high throughput technique for analysis of tr

5 Tn-Seq is an experimental method for probing the functio

6 Tn-seq is based on the assembly of a saturated Mariner t

7 Tn-seq measures the frequency of actual members of a het

8 Tn-seq was used to identify genes essential for phototro

9 In the absence of Ca(2+), Tn that is tightly bound to Tpm binds actin and holds th

10 (WT) Tn, non-phosphorylatable cTnI (S23/24A) Tn or phosphomimetic cTnI (S23/24D) Tn.

11 S23/24A) Tn or phosphomimetic cTnI (S23/24D) Tn.

12 employing 22 million Europeans in a euro 1.5 Tn endeavour, being the premier global economic growth o

13 C. burnetii clone was isolated containing a Tn insertion within the C terminus of the cell division

14 nTHyp was not evaluated as it did not form a Tn complex under a variety of conditions.

15 One STM mutant, JG736, with a Tn insertion in lpp, encoding Braun's lipoprotein, was c

16 lized B cell line from a male patient with a Tn-syndrome-like phenotype.

17 h may be important in understanding aberrant Tn antigen expression in human diseases, including IgA n

18 In addition, Tn mutants of 14 genes displayed enhanced piglet infecti

19 In the adult Tn environment (cTnT3 + cardiac troponin I), the single

20 ing of the intensity profiles obtained after Tn exchange at pCa 5.8 suggest that the profiles are bes

21 tic efficacy of CAR T cells directed against Tn-MUC1 and present aberrantly glycosylated antigens as

22 molecule, also known as EpCAM) and mucin Ags Tn, sialylated Tn, Thomsen Friedenreich (TF), and MUC1 a

23 also terminal Ser/Thr O-linked alphaGalNAc (Tn antigen).

24 r of ways that the periodic occurrence of An/Tn clusters could reflect evolution and function of gene

25 s, characterized by 10-base pair periodic An/Tn-clusters (PATCs), can license transgenes for germline

26 me, indicating a dynamic character to the An/Tn periodicity.

27 cal methods have been proposed for analyzing Tn-Seq data to identify genetic interactions, including

28 te HMM provides an improved way of analyzing Tn-seq data and assessing different levels of essentiali

29 s, Tn(c)-keyhole limpet hemocyanin (KLH) and Tn(c)-palmitic acid (PAM) with the saponin immunologic a

30 gene in mice causes embryonic lethality and Tn antigen expression.

31 ohydrate antigens, Globo-H, GM2, STn, TF and Tn-to maleimide-modified carrier protein KLH.

32 These results indicate that both Tm and Tn enhance the force generated by each cross-bridge, and

33 analysis available for most transposons and Tn-Seq associated approaches (e.g. TraDis, HiTS, IN-Seq)

34 Anti-Tn-MUC1 CAR T cells demonstrated target-specific cytotox

35 (ZPS) PS A1 and the well-known tumor antigen Tn has been designed, synthesized, and studied for immun

36 contains prostate tumor-associated antigens, Tn, TF, STn, Lewis(y), and Globo-H.

37 mmon tumor-associated carbohydrate antigens, Tn and sialyl Tn (STn), result from somatic mutations in

38 s of tumor-associated carbohydrate antigens, Tn and STn, were assembled on a single cyclic peptide sc

39 a cluster of the most common TACA (known as Tn antigen) covalently attached to T-cell peptide epitop

40 oped a CAR that recognized cancer-associated Tn glycoform of MUC1, a neoantigen expressed in a variet

41 The production of the autoimmune Tn antigen by a glycosyltransferase enzyme rendered defe

42 melanoma-derived cells lines, expressed both Tn and STn antigen due to loss-of-function mutations in

43 f human gut epithelial cells was assessed by Tn-insertion site sequencing (Tn-seq).

44 role of eight additional genes identified by Tn-seq in A. baumannii resistance to killing by NHS but

45 nditionally essential proteins identified by Tn-seq were analyzed by targeted proteomics, and 70% of

46 , a cytokine controlling IL-17 production by Tn cells.

47 tion of a cardiac biomarker, troponin I-T-C (Tn I-T-C) complex, was developed.

48 cal model in which strong A-M binding and Ca-Tn binding independently activates the rate of A-M weak-

49 on actin either through calcium-troponin (Ca-Tn) binding or by actin-myosin (A-M) strong binding.

50 und to be > 2-fold faster from whole cardiac Tn compared with skeletal Tn.

51 These neutrophil-regulatory T cells (Tn) are expanded in mice that lack leukocyte adhesion mo

52 or cells exposing both NA2/NA3 and clustered Tn structures.

53 actosamine (GalNAc), the so-called clustered Tn antigen, a cancer-specific O-glycan on mucins.

54 A3-presenting asialofetuin and the clustered Tn-rich asialo-bovine submaxillary mucin, were subsequen

55 netic interactions, which involves comparing Tn mutant libraries generated in different genetic backg

56 MAGenTA is a complete Tn-Seq analysis pipeline making sensitive genome-wide fi

57 esponse to immunization with two conjugates, Tn(c)-keyhole limpet hemocyanin (KLH) and Tn(c)-palmitic

58 s work, a transposon insertion mutant (cpsA::Tn) of Mycobacterium marinum was studied.

59 prior to this work, the size of the discrete Tn cluster remained at T3, with only 10 metal sites (e.g

60 ss-reactive toward mucin proteins displaying Tn.

61 At each SL, F(max) was unaffected by either Tn exchange and/or PKA treatment.

62 nge in rigor myofibrils was used to estimate Tn dissociation rates from the nonoverlap and overlap re

63 as tested on simulated data and experimental Tn-Seq data from Serratia marcescens transposon mutant l

64 Thus far, Tn clusters up to T4 with 20 metal sites can be synthesi

65 In the fetal Tn environment, (cTnT1 + slow skeletal troponin I), the

66 For Tn-seq, a saturated Tn5 insertion library was grown in v

67 Novel statistical method for Tn-seq data analysis is needed to infer functions of gen

68 The higher affinities of SBA and VML for Tn-PSM relative to Fd-PSM indicate the importance of car

69 The higher affinities of SBA and VML for Tn-PSM relative to its two shorter chain analogs demonst

70 The resulting data from Tn-seq experiments consist of sequence reads mapped to m

71 gests that Ca(2+) binding to each functional Tn activates < 7 actins of a structural regulatory unit

72 y exposed in the absence of core 1 O-glycan, Tn antigen, in the colon epithelium of a subset of UC pa

73 highly efficient intersystem-crossing S1 --> Tn and phosphorescence T1 --> S0.

74 ivity had transposon insertions in hha (hha::Tn).

75 Specific complementation of the hha::Tn mutation with a plasmid-encoded copy of hha reduced b

76 osamine-transferases (GalNAc-Ts) drives high Tn levels in cancer cell lines and in 70% of malignant b

77 h Ca(2+) dissociated from site II of cTnC in Tn complexes also depended on the cTnT isoform present.

78 al for core 1 O-glycosylation, were found in Tn-positive epithelia.

79 identifying conditionally essential genes in Tn-Seq experiments with high detection sensitivity and s

80 eletal) muscle, Ca(2+) binding to individual Tn complexes is insufficient to completely activate thei

81 , screened genome-wide transposon insertion (Tn-seq) and transcriptome (RNA-seq) libraries to charact

82 Compared with HCTnT3 (adult isoform), Tn complexes containing HSSTnT1, -2, and -3 did not alte

83 All rod-shaped C. jejuni Tn mutants and all rod-shaped laboratory, clinical and e

84 se activity as a function of pCa and labeled Tn exchange in rigor myofibrils was used to estimate Tn

85 The time course of labeled Tn exchange at pCa 9 and 4 were quite different between

86 from rigor skeletal myofibrils using labeled Tn exchange.

87 However, the synthesis of large Tn clusters is a significant challenge, and for several

88 fluorescently labeled Tm to directly measure Tn binding, we show that binding of Tn to both isoforms

89 high-throughput strategy based on the method Tn-seq that can be applied to any genetically manipulata

90 Here we present a method (Tn-seq) for accurately determining quantitative genetic

91 These T4 clusters are the largest molecular Tn clusters known to date and can be made in various com

92 elial tumor marker MUC1 carrying one or more Tn, T, or sialyl-T antigens.

93 II-phosphorylated Tn complex with the native Tn complex in the fiber bundles resulted in inhibition o

94 n concomitant Ca(2+) binding at neighbouring Tn sites and/or crossbridge feedback effects on Ca(2+) b

95 synthase instead expressed the nonsialylated Tn antigen in these cells and developed brain hemorrhage

96 is specifically designed for the analysis of Tn-Seq data.

97 An important emerging application of Tn-Seq is for identifying genetic interactions, which in

98 gnificant changes occurred in the binding of Tn containing the RCM cTnT mutation to actin-Tm.

99 measure Tn binding, we show that binding of Tn to both isoforms is similar (0.1-0.5 microm) and both

100 alNAc residue along the polypeptide chain of Tn-PSM before dissociating.

101 Comparison of Tn-seq results from laboratory cultures and from monoino

102 erived 81 amino acid tandem repeat domain of Tn-PSM containing approximately 23 alpha-GalNAc residues

103 The location and rate of exchange of Tn or its subunits were measured by high-resolution fluo

104 chaperone Cosmc, favoring the expression of Tn antigen.

105 Ag/AgCl reference electrode as a function of Tn I-T-C complex concentration during incubations.

106 egy that incurs significant incorporation of Tn antigen.

107 ost malignant tumors have elevated levels of Tn, an O-GalNAc glycan.

108 ine, which could be considered as a mimic of Tn antigen.

109 Our results highlight the power of Tn-seq for the discovery of new genes and novel pathways

110 th the hypothesis that Tm in the presence of Tn and Ca2+ exerts a positive allosteric effect on actin

111 was tested by measuring the apparent rate of Tn dissociation from rigor skeletal myofibrils using lab

112 The rate of Tn dissociation in the non-overlap region was 200-fold f

113 At pCa 9, the rate of Tn dissociation in the overlap region (M-state region) w

114 hanism of calcium enhancement of the rate of Tn dissociation is by favoring a TnI-TnC interaction ove

115 At pCa 4, the rate of Tn dissociation was twofold faster in the non-overlap re

116 at strong cross-bridges increase the rate of Tn dissociation.

117 Three different rates of Tn exchange were observed that were dependent on calcium

118 ficant effect upon the calcium regulation of Tn.

119 This report reveals the protective role of Tn fetal isoforms as they rescue the increased Ca(2+) se

120 R-regulated TTSS repressor, another round of Tn mutagenesis was carried out in the background of a pr

121 ived 38/40 amino acid cleavage product(s) of Tn-PSM containing approximately 11-12 alpha-GalNAc resid

122 Depending on the activation state of Tn and the binding state of myosin, Tm can occupy the bl

123 re associated with three different states of Tn.

124 nin (Tn) isoform expression and/or status of Tn phosphorylation, we studied fiber bundles that had be

125 ange influence of the strong cross-bridge on Tn to enhance its dissociation rate, tens of nanometers

126 timulation of cell migration is dependent on Tn-bearing proteins present in lamellipodia of migrating

127 Calcium has little effect on Tn binding to the actin.Tm complex and both exons produc

128 lts that show exon 6 has little influence on Tn affinity to actin.Tm or its ability to fully inhibit

129 An ordered Tn library in strain AB5075 with insertions in every non

130 However, the affinity of bound SBA for other Tn-PSM molecules during cross-linking is much higher tha

131 ed chaperone may have implications for other Tn-related disorders such as IgA nephropathy, a conditio

132 ty of bound SBA for GalNAc residues on other Tn-PSM molecules appears to be due to the favorable entr

133 estyle to bloodstream invasion, we performed Tn-seq on GBS strain A909 with human whole blood.

134 Moreover, exchange of ROCK-II-phosphorylated Tn complex with the native Tn complex in the fiber bundl

135 A controls the expansion of IL-17A-producing Tn cell populations through IL-17R.

136 ecretion, and the number of IL-17A-producing Tn cells were elevated in Il17ra(-/-) and Il17ra(-/-)Itg

137 The total number of CD3+ IL-17A-producing Tn cells were significantly reduced in the spleen and la

138 iesis and the prevalence of IL-17A-producing Tn cells.

139 Me, L = Cl(-), NCS(-), NCO(-), N(3)(-); R' = Tn, L = Cl(-), NCS(-).

140 e endogenous cTn was replaced by recombinant Tn.

141 y expressed fast skeletal muscle recombinant Tn.

142 s, restores T-synthase activity, and reduces Tn antigen expression.

143 ough IL-17A-producing neutrophil regulatory (Tn) cells, most of which express gammadelta TCR.

144 in mZP3) are exclusively core 1 and related Tn sequences, whereas core 2 O-glycans predominate at th

145 show that expression of the disease-related Tn antigen can result from deregulation or loss of Cosmc

146 nd approximately 2300 alpha-GalNAc residues (Tn-PSM) binds to the soybean agglutinin (SBA) with a K(d

147 mately 2300 GalNAcalpha1-O-Ser/Thr residues (Tn-PSM) has been ascribed to an internal diffusion mecha

148 d be regarded as conformationally restricted Tn antigen mimics, as we have demonstrated by biological

149 n (Tm) resulting from changes in troponin's (Tn) interaction with actin-Tm: a blocked (B) state preve

150 binds MUC1 that carries the Tn or sialyl (S)Tn glycan.

151 tigated the DNA methylation pattern of an SB Tn as well as the flanking genomic region at insertion s

152 aken together, these results suggest that SB Tn insertions into the mouse genome can be discriminated

153 l part of the eGFP coding sequence in the SB Tn exhibited high levels of CpG methylation in transgeni

154 oring chromosomal sequences of two unique SB Tn insertions compared to wild-type patterns.

155 s gamma-globin gene were achieved using a SB-Tn beta-globin cis construct.

156 suggested that a fluorescent reporter cis SB-Tn system can be used to enrich mammalian cells harborin

157 ere a novel dual fluorescent reporter cis SB-Tn system that permitted nonselective fluorescent-activa

158 We then constructed cis SB-Tn-beta-globin plasmids using a minimal beta-globin gene

159 ant enrichment (>60%) of cells exhibiting SB-Tn-mediated genomic insertions and long-term expression

160 ve fluorescent-activated cell sorting for SB-Tn-transduced K562 erythroid cells.

161 The SB-Tn system is a promising nonviral vector for efficient g

162 Sleeping Beauty transposon (SB-Tn) has emerged as an important nonviral vector for inte

163 Using a high-throughput screen (Tn-seq), we identified genes in recipients that contribu

164 ere assessed using ToxT and in vivo RNA-seq, Tn-seq, and cholera stool proteomic and other genome-wid

165 transposon mutagenesis and deep sequencing (Tn-seq) to identify T6SS immunity proteins in Vibrio cho

166 Transposon insertion sequencing (Tn-Seq) is a microbial systems-level tool, that can dete

167 Transposon insertion sequencing (Tn-seq) is an emerging technology that combines transpos

168 e used transposon insertion site sequencing (Tn-seq) to comprehensively assess the contribution of ne

169 as assessed by Tn-insertion site sequencing (Tn-seq).

170 next-generation high-throughput sequencing (Tn-seq) promises to revolutionize systems level analysis

171 um resistance, a transposon (Tn) sequencing (Tn-seq) approach was used to identify genes contributing

172 previously reported a transposon sequencing (Tn-seq) system for performing genomewide assessment of g

173 A screen using transposon sequencing (Tn-seq) was performed to search for genes within ExPEC i

174 Here, we used transposon-sequencing (Tn-seq) to assess whether there were any sporulation gen

175 tory factors, we used transposon-sequencing (Tn-Seq)(5) to screen for mutations affecting the growth

176 ced affinity relative to GalNAcalpha1-O-Ser (Tn), the pancarcinoma carbohydrate antigen.

177 ociated carbohydrate antigens, Tn and sialyl Tn (STn), result from somatic mutations in the gene Cosm

178 scale production of MUC1 carrying 83% sialyl-Tn O-glycans.

179 scrimination of the cancer-associated sialyl-Tn (STn) antigen was developed by using Sambucus nigra a

180 alylated O-glycans, the disaccharide, sialyl-Tn (sialic acid alpha2,6GalNAc), is expressed by 30% of

181 corporation into the carcinoma marker Sialyl-Tn, and is the first example of such a novel mechanism f

182 ing ST6GalNAc-I and the expression of sialyl-Tn is evident, demonstrating that the expression of sial

183 demonstrating that the expression of sialyl-Tn results from switching on expression of hST6GalNAc-I.

184 ) always results in the expression of sialyl-Tn.

185 ertain mucins and weak binding to the sialyl-Tn epitope.

186 known as EpCAM) and mucin Ags Tn, sialylated Tn, Thomsen Friedenreich (TF), and MUC1 all reacted comp

187 recombinant Tm mutants and purified skeletal Tn.

188 from whole cardiac Tn compared with skeletal Tn.

189 Small Tn clusters can also be synthesized in discrete forms, a

190 The snm9::Tn mutant phenocopies bona fide snm mutants, exhibiting

191 C-ms proteomics), gene essentiality studies (Tn-seq), genome wide protein localization studies, and g

192 We present herein several sulfa-Tn antigens incorporated in MUC1 sequences that possess

193 Supertetrahedral Tn clusters are exact fragments of a cubic ZnS type latt

194 Supertetrahedral Tn clusters are exact fragments of cubic ZnS-type lattic

195 sents the largest molecular supertetrahedral Tn cluster known to date.

196 mmune responses derived from single naive T (Tn) cells, single primary, and single secondary central

197 f IL-17A-expressing neutrophil-regulatory T (Tn) cells; CD4(-)CD8(-)alphabeta(low), CD4(+)CD8(-)alpha

198 ocked down in ESCC cell lines (KYSE450 and T.Tn), immortalized normal esophageal epithelial cell line

199 Here we show that Tn syndrome is associated with a somatic mutation in Cos

200 The Tn complex consists of three subunits, troponin C (TnC),

201 The Tn-PS A1 conjugate construct confers specificity toward

202 ero-inflated Poisson model for analyzing the Tn-seq data that are high-dimensional and with an excess

203 In cancer cells, some glycans (such as the Tn antigen) are highly up-regulated, but this remains la

204 n with terminal GalNAc moieties, such as the Tn antigen, on CD45 of effector T cells, thereby down-re

205 osylated membrane glycoprotein, known as the Tn antigen.

206 tures of MUC1-like glycopeptides bearing the Tn antigen (alpha-O-GalNAc-Ser/Thr) in complex with an a

207 Identification of the genes disrupted by the Tn revealed 24 distinct loci required for anaerobic grow

208 CAR selectively binds MUC1 that carries the Tn or sialyl (S)Tn glycan.

209 lls lack T-synthase activity and express the Tn antigen.

210 ies of mucins including those expressing the Tn cancer antigen.

211 of a scFv antibody fragment specific for the Tn-glycoform of MUC1 had potent activity in preclinical

212 or Thr residues of core proteins to form the Tn antigen (GalNAc-alpha-1-O-Ser/Thr).

213 ase, the only enzyme that galactosylates the Tn antigen (GalNAcalpha1-Ser/Thr-R) to form core 1 Galbe

214 g synthetic glycopeptides with O-GalNAc (the Tn antigen) or O-GlcNAc, we demonstrated that the method

215 address this question in the context of the Tn 10 transposition reaction, in which the DNA cleavage

216 T-synthase and consequent expression of the Tn antigen (GalNAcalpha1-Ser/Thr), which is associated w

217 sm underlying the abnormal expression of the Tn antigen, which may be important in understanding aber

218 evidence that ROCK-II phosphorylation of the Tn complex, most likely at cTnT, has an important role i

219 N-lobe to rotate relative to the rest of the Tn molecule.

220 A polymerizable version of the Tn-antigen glycan was prepared and converted into well-d

221 A special feature of the Tn-seq data is that multiple mutants in a gene provides

222 ncer specimens that showed expression of the Tn/STn antigens were also found to have mutations in Cos

223 osin ATPase activity than that of TnC or the Tn complex.

224 tion of antibodies that are selective to the Tn-antigen glycan and cross-reactive toward mucin protei

225 ate construct confers specificity toward the Tn antigen alone, and specific carbohydrate immunoglobul

226 The analysis used the Tn-seq circle method to achieve high genome coverage and

227 the identification of glycopeptides with the Tn antigen by mass spectrometry.

228 hod, we identified 96 glycoproteins with the Tn antigen in Jurkat cells.

229 st designed to enrich glycoproteins with the Tn antigen.

230 ion (R = tert-butyl, R' = H, Me, 2'-thienyl (Tn), L = Cl(-), NCS(-), NCO(-), N(3)(-)), has been chara

231 riation and morphological trends within this Tn library, and in various C. jejuni wild type strains,

232 rs Gal from UDP-Gal to GalNAcalpha1-Ser/Thr (Tn antigen) to form the core 1 O-glycan Galbeta1-3GalNAc

233 lence determines the affinities of the three Tn-PSM analogs.

234 time course of myosin-S1 binding to actin-Tm-Tn filaments in solution at various calcium levels with

235 n of native Tm and troponin (Tn) complex (Tm-Tn) in cardiac myofibril preparations has been developed

236 The endogenous Tm-Tn complex was selectively removed from myofibrils and r

237 udies showed that 1) depletion of >80% of Tm-Tn from myofibrils resulted in a complete loss of the Ca

238 2) reconstitution of exogenous wild-type Tm-Tn resulted in complete regain in the calcium regulation

239 reduction in the S1 binding rate to actin.Tm.Tn in its absence.

240 Our approach was applied to Tn-Seq libraries made in isogenic strains of Mycobacteri

241 Ca(2+) binding to Tn releases the Tpm from actin so that it moves azimutha

242 le contraction by coupling Ca(2+) binding to Tn with myosin binding to the thin filament.

243 teractions and favored by calcium binding to Tn, and an open or M state allowing strong myosin intera

244 ing negative cooperativity of SBA binding to Tn-PSM correlates with a decreasing level of internal di

245 Transposon (Tn) gene-inactivation libraries were generated in three

246 Of 3,850 transposon (Tn) mutants screened, 46 were identified as colonization

247 pC, we identified mutants from a transposon (Tn) insertion library which lack surface-exposed Ebp pil

248 strointestinal tract utilising a transposon (Tn) mutant library screen.

249 A. baumannii serum resistance, a transposon (Tn) sequencing (Tn-seq) approach was used to identify ge

250 enerated by mariner-based Himar1 transposon (Tn) mutagenesis.

251 A C. jejuni transposon (Tn) mutant library was screened for non-helical mutants

252 red for anaerobic growth, random transposon (Tn) mutagenesis was used to screen for mutants that demo

253 d potential drug targets, random transposon (Tn) mutants derived from the A. baumannii strain AB307-0

254 The Sleeping Beauty (SB) transposon (Tn) system is a nonviral gene delivery tool that has wid

255 system, the Sleeping Beauty (SB) transposon (Tn) to insert a wild-type beta-globin expression cassett

256 the wide activity of the Mariner transposon, Tn-seq has the potential to contribute to the exploratio

257 t least in part by the troponin-tropomyosin (Tn-Tm) switch on the thin (actin-containing) filaments.

258 Troponin (Tn) complexes containing ssTnI and reconstituted with cT

259 Troponin (Tn) is an important regulatory protein in the thin-filam

260 Troponin (Tn) is the calcium-sensing protein of the thin filament.

261 on/reconstitution of native Tm and troponin (Tn) complex (Tm-Tn) in cardiac myofibril preparations ha

262 nd Lethocerus tropomyosin (Tm) and troponin (Tn) regulate the binding of rabbit myosin to rabbit acti

263 ils by replacing endogenous Tm and troponin (Tn) with recombinant Tm mutants and purified skeletal Tn

264 , myosin-S1, tropomyosin (Tm), and troponin (Tn).

265 en years ago, mutations in cardiac troponin (Tn)T and alpha-tropomyosin were linked to familial hyper

266 force-pCa relationship, endogenous troponin (Tn) was exchanged in rat ventricular trabeculae with eit

267 To rule out any alterations in troponin (Tn) isoform expression and/or status of Tn phosphorylati

268 In skeletal and cardiac muscles, troponin (Tn), which resides on the thin filament, senses a change

269 ropomyosin (Tm) in the presence of troponin (Tn) and Ca2+ enhances both force and velocity, and a tru

270 uence data suggest the presence of troponin (Tn) components, consistent with actin-linked regulation,

271 nteraction with and functioning of troponin (Tn) has been studied using recombinant fibroblast Tm iso

272 The inhibitory region of troponin (Tn)I differs by a single residue, proline at position 11

273 ding and dissociation of Ca(2+) on troponin (Tn) to the movement of tropomyosin on actin filaments.

274 Ca(2+) via the regulatory proteins troponin (Tn) and tropomyosin (Tpm), which are associated with act

275 ase the calcium sensitivity of the troponin (Tn) complex or reconstituted thin filaments with or with

276 of synthetic filaments of actin-Tm-Troponin (Tn), and weakens Tm binding to the actin-myosin subfragm

277 ning of Tm along the actin (Ac):Tm:troponin (Tn) thin filament to block or expose myosin binding site

278 was further reconstituted without troponin (Tn), and the kinetic constants of the elementary steps o

279 Here, we use tunicamycin (Tn) to investigate ER stress-triggered changes in the tr

280 Mechanisms underlying Tn up-regulation and its effects remain unclear.

281 Here we use Tn-seq to assess gene function in the Gram negative gamm

282 Here, we used Tn-seq to identify genes important for resistance to com

283 ysis of a saturated mutant GAS library using Tn-sequencing and generation of a conditional-expression

284 sequenced in a high throughput manner using Tn-seq to identify potential mechanisms of xds regulatio

285 using Sanger sequencing and in a pool using Tn-seq.

286 on bottlenecks in a murine host, we utilized Tn-seq to monitor the composition of mixed populations o

287 When Tn contained engineered TnC mutants with weakened regula

288 imilar reduction also could be observed when Tn contained cTnI(Thr>Pro) (deltaEC50=0.24+/-0.04 microm

289 A total of 123 genes of which Tn mutants showed attenuated piglet infection were ident

290 a(2+) dissociation rates (k(off)) from whole Tn complexes containing sTnC (26 +/- 0.1 s(-1)), cTnC (3

291 vors exhibited abnormalities correlated with Tn antigen expression that are related to several human

292 A murine SCD model coupled with Tn-seq mutagenesis identified 60 noncapsular pneumococca

293 to the value of the control myocardium with Tn reconstitution.

294 ated form of Cosmc observed in patients with Tn syndrome has reduced chaperone function.

295 Thin filaments reconstituted with Tn and Tm5a or skeletal Tm (containing exon 6b) show nea

296 ed myocardium was further reconstituted with Tn, and the effect of MgATP on the rate constants (K(1),

297 Following reconstitution with Tn, the myocardium regained the Ca(2+)-sensitivity and t

298 Following treatment with Tn, HuR binding to cytochrome c mRNA decreased, and both

299 function, their subunit interactions within Tn and with actin-tropomyosin are different.

300 icular trabeculae with either wild-type (WT) Tn, non-phosphorylatable cTnI (S23/24A) Tn or phosphomim