ライフサイエンスコーパス: Torpedo

1 c acetylcholine receptor-rich membranes from Torpedo.

2 with torpedoes versus Purkinje cells without torpedoes.

3 ted shows 94% homology (86% identity) to the Torpedo 87 kDa protein and 50% homology to the cysteine-

4 In addition, antibodies against either the Torpedo 87 kDa protein or human dystrobrevin demonstrate

5 utrophin/dystrophin related protein, and the Torpedo 87K protein.

6 ibody 383C binds to the alpha subunit of the Torpedo acetylcholine (ACh) receptor as well as to its V

7 t a refined model of the membrane-associated Torpedo acetylcholine (ACh) receptor at 4A resolution.

8 olesterol on the ion-channel function of the Torpedo acetylcholine receptor (nAChR) and the novel lip

9 Immunization with Torpedo acetylcholine receptor (TAChR) induces experimen

10 induced in C57Bl/6 mice by immunization with Torpedo acetylcholine receptor (TAChR), to demonstrate t

11 Mice expressing the Torpedo acetylcholine receptor alpha-chain as a neo-self

12 el gating by using a structural model of the Torpedo acetylcholine receptor at 4-A resolution, record

13 Based on the Torpedo acetylcholine receptor structure, Unwin and coll

14 lity of residues alpha187 to alpha199 of the Torpedo acetylcholine receptor with monoclonal antibody

15 The mice were immunized with Torpedo acetylcholine receptor, and EAMG was assessed by

16 ns with identical peptide/MHC specificity in Torpedo acetylcholine receptor-alpha-transgenic animals

17 modified to either F (KI = 0.6 microM, as in Torpedo acetylcholinesterase) or Y (KI = 0.032 microM, a

18 For Torpedo AChE, a K(S) of 0.5+/- 0.2 mM obtained from subs

19 the carboxyl terminus in comparison with the Torpedo AChE, and three of the 14 aromatic residues that

20 l by measuring the inhibition constants with Torpedo AChE, fetal bovine serum AChE, human butyrylchol

21 ds differently to human AChE than it does to Torpedo AChE.

22 el was generated in which the immunodominant Torpedo AChR (T-AChR) alpha subunit is expressed in appr

23 d in C57BL6 (B6) mice by three injections of Torpedo AChR (TAChR) in adjuvant.

24 ockout mutants (IFN-gamma-/-, IL-12-/-) with Torpedo AChR (TAChR).

25 a previously unsuspected interaction between Torpedo AChR and the adaptor protein Grb2.

26 gene knockout (KO) mice were immunized with Torpedo AChR in CFA.

27 ha-bungarotoxin-bound AChR, (e) binds to the Torpedo AChR in either state mainly by an entropy-driven

28 es at the ACh binding sites of the mouse and Torpedo AChR shows mouse residue Ile-gamma116 as likely

29 this change does not affect affinity for the Torpedo AChR sites.

30 that TG mice had reduced activation of anti-Torpedo AChR Th1 cells, but increased anti-AChR Ab synth

31 ne to the desensitized/carbamylcholine-bound Torpedo AChR with higher affinity compared to the restin

32 that (i) CrV interacts with the desensitized Torpedo AChR with higher affinity than with the resting

33 e equilibrium binding affinity of [3H]ACh to Torpedo AChR-rich membranes.

34 ng affinity to the mouse muscle AChR and the Torpedo AChR.

35 eferred the desensitized conformation of the Torpedo AChR.

36 reversibly inhibited ACh-induced currents of Torpedo AChRs expressed in Xenopus oocytes.

37 d maintenance of Delta expression by veinlet/torpedo activity.

38 muscle and a neuronal nAChR, we photolabeled Torpedo alpha(2)betagammadelta and expressed human alpha

39 4-191 with the corresponding region from the Torpedo alpha1 subunit, we introduced a cluster of five

40 and Phe227 at the lipid-exposed face of the Torpedo alpha1M1 segment.

41 ABAAR potentiator, has been shown to inhibit Torpedo alpha2betagammadelta nAChRs, binding in the ion

42 its human muscle (alphabetaepsilondelta) and Torpedo (alphabetagammadelta) nAChR expressed in Xenopus

43 of mouse AChR, whereas the affinity for the Torpedo alphagamma-site was slightly increased.

44 e photolabeled alpha4Tyr(195) (equivalent to Torpedo alphaTyr(190)) in Segment C as well as beta2Val(

45 potential energy profiles are seen when the Torpedo and alpha 7 models are compared.

46 o, cell membranes from the electric organ of Torpedo and from the rat brain were transplanted to frog

47 non-hair identity in the transition between torpedo and mature stage.

48 alphaA-conotoxins EIVA and EIVB, block both Torpedo and mouse alpha1-containing muscle subtype nAChR

49 Both psi-conotoxins block Torpedo and mouse nicotinic acetylcholine receptors (nAC

50 hose mediated by the transmembrane receptors Torpedo and Notch.

51 frog oocytes, which thus acquired functional Torpedo and rat neurotransmitter receptors.

52 oned in humans; COLQ cDNA has been cloned in Torpedo and rodents but not in humans.

53 d dropout of Purkinje cells, Purkinje axonal torpedoes and Bergmann gliosis.

54 levels of IFs and abnormal organelles in the torpedoes and soma of Purkinje cells, as well as in the

55 egraded by 5' --> 3' exonuclease activities (torpedoes) and so induces dissociation of Pol II from th

56 These torpedoes are abnormal swellings of Purkinje cell axons

57 tem studies, Purkinje cell axonal swellings (torpedoes) are present to a greater degree in essential

58 exin resulted in the formation of cerebellar torpedoes as early as 1 month of age.

59 ell epitope within residues alpha 146-162 of Torpedo californica (t), tAChR, plays an important role

60 Cocrystallization of the enzyme from Torpedo californica (TcCK) with ADP-Mg(2+), nitrate, and

61 n the C57BL/6 background were immunized with Torpedo californica acetylcholine receptor (AChR) and ev

62 ipid-exposed transmembrane segment M4 of the Torpedo californica acetylcholine receptor (AChR) focuse

63 n mice, EAMG is induced by immunization with Torpedo californica acetylcholine receptor (AChR) in com

64 e assessed in transgenic mice expressing the Torpedo californica acetylcholine receptor (TAChR) alpha

65 h the aged and nonaged crystal structures of Torpedo californica acetylcholinesterase inhibited by th

66 For Torpedo californica acetylcholinesterase, monomeric and

67 Although BSF inhibits both mouse and Torpedo californica AChE, PMSF does not react measurably

68 y structure of a transition-state complex of Torpedo californica AChE-m-(N,N,N-trimethylammonio)-2,2,

69 long-chain alkanol sites on the desensitized Torpedo californica AChR and to investigate if these sit

70 fragments comprising residues 143-210 of the Torpedo californica alpha-subunit were expressed in E. c

71 o acid substitutions at the M4 domain of the Torpedo californica and mouse acetylcholine receptor sug

72 of the homologous acetylcholinesterase from Torpedo californica complexed with TMTFA (2.66 +/- 0.28

73 ent upon binding to AChR-rich membranes from Torpedo californica electric organ.

74 cotinic acetylcholine receptors (nAChR) from Torpedo californica electric tissue in their membrane-bo

75 ntial enrichment methodology and the AChR in Torpedo californica electroplax membranes were used to f

76 In this regard, we used the Torpedo californica nAChR and a series of barbiturate an

77 technique their interaction with the AChBP, Torpedo californica nAChR and chimeric receptor composed

78 s contributing to their binding sites in the Torpedo californica nAChR.

79 e and kinetics of azietomidate inhibition of Torpedo californica nAChRs and time-resolved photolabeli

80 as used to examine structural changes in the Torpedo californica nicotinic acetylcholine receptor (AC

81 r more transmembrane segments (M1-M4) of the Torpedo californica nicotinic acetylcholine receptor (AC

82 To further define the surface of the Torpedo californica nicotinic acetylcholine receptor (nA

83 oclonal antibodies (mAbs) on the function of Torpedo californica nicotinic acetylcholine receptor (nA

84 The lipid requirements of the Torpedo californica nicotinic acetylcholine receptor (nA

85 esterol) was used to identify domains in the Torpedo californica nicotinic acetylcholine receptor (nA

86 s EST is also highly homologous (90%) to the Torpedo californica post-synaptic 87 kDa phosphoprotein.

87 The AChR from Torpedo californica was labeled with a fluorescent probe

88 nicotinic acetylcholine receptor (AChR) from Torpedo californica were measured using sequential-mixin

89 nicotinic acetylcholine receptor (AChR) from Torpedo californica were used to determine binding chara

90 vesicles isolated from the electric organ of Torpedo californica, a model cholinergic synapse, contai

91 ith heterologous AChR from the electric fish Torpedo californica, has been used extensively.

92 nicotinic acetylcholine receptor (AChR) from Torpedo californica.

93 an the nicotinic acetylcholine receptor from Torpedo californica.

94 les were isolated from the electric organ of Torpedo californica.

95 hloride channel belonging to the CIC family (Torpedo CIC-0) has functional features that suggest that

96 napologetically subjective essay recalls the Torpedo Cl(-) channel in the years when it had neither a

97 The Drosophila gene torpedo/Egfr (top/Egfr) encodes a homolog of the vertebr

98 ng oocyte and is thought to locally activate torpedo/Egfr (top/Egfr), the Drosophila homolog of the E

99 nicotinic acetylcholine receptor (AChR) from Torpedo electric organ and mammalian muscle contains hig

100 In addition, we isolated MuSK from Torpedo electric organ and used nanoelectrospray tandem

101 odot assays, synaptic vesicles purified from Torpedo electric organ are also immunoreactive for PMCA

102 nicotinic acetylcholine receptor (nAChR) in Torpedo electric organ membranes.

103 ]APFBzcholine with nAChR-rich membranes from Torpedo electric organ revealed equal affinities (K(eq)

104 ed on dystroglycan and other constituents of Torpedo electric organ synaptic membranes.

105 choline receptor (nAChR)-rich membranes from Torpedo electric organ with [(14)C]halothane and determi

106 choline receptor (nAChR)-rich membranes from Torpedo electric organ with a photoactivatable analog, [

107 rs as a single 2.4 kb transcript abundant in Torpedo electric organ, moderately expressed in spinal c

108 f ClC-0, a chloride (Cl(-)) channel from the Torpedo electric organ.

109 ding partners in postsynaptic membranes from Torpedo electric organ.

110 e of electrocytes in isolated columns of the Torpedo electric organ.

111 EM image of the transmembrane domain of the torpedo electric ray nicotinic channel, we were provided

112 The immobilization of Torpedo electrocyte membranes on the surface of micropla

113 method development was the immobilization of Torpedo electrocyte membranes rich in nicotinic acetylch

114 ized with the AChR on the innervated face of Torpedo electrocytes.

115 ously identified noncompetitive inhibitor of Torpedo electroplax nAChR, also isolated from C. purpura

116 Studies on Torpedo electroplax nicotinic acetylcholine (ACh) recept

117 otinic acetylcholine receptors (nAChRs) from Torpedo electroplax, using (19)F nuclear magnetic resona

118 n AChE) increased the K(S) to 4-10 mM in the Torpedo enzyme and to about 33 mM in the human enzyme.

119 ors phosphorylated by Cdk9 was the 5'-to-3' "torpedo" exoribonuclease Xrn2, required in transcription

120 nd beta2Ser(113) in Segment E (equivalent to Torpedo gammaLeu(109) and gammaTyr(111), respectively).

121 e and the Drosophila EGF receptor homologue, Torpedo, in the surrounding somatic follicle cells.

122 sis of the macula or peripapillary area and "torpedo-like" lesions along the vascular arcades may als

123 ew clinical observations in ESCS include (1) torpedo-like, deep atrophic lesions with a small hyperpi

124 atrophy that now can be expanded to include torpedo maculopathy, vascular changes, and hemorrhagic r

125 nts can be suppressed by the introduction of Torpedo marmorata CLC-0 or Arabidopsis thaliana CLC-c an

126 was developed based on the recently refined Torpedo marmorata nACh receptor.

127 BP) and the transmembrane (TM) domain of the Torpedo marmorata nAChR.

128 ves were considered as binding proteins, the Torpedo marmorata nicotinic acetylcholine receptor (nACh

129 ys-loop receptors based on homology with the Torpedo marmorata nicotinic acetylcholine receptor infer

130 of the nicotinic acetylcholine receptor from Torpedo marmorata, reveals an asymmetric ion channel wit

131 receptor isolated from the electric organ of Torpedo marmorata.

132 stone and U snRNA genes, suggesting that the torpedo mechanism is not limited to poly(A) site-depende

133 ranscripts and premature termination by the "torpedo" mechanism is a widespread mechanism that limits

134 However recent in vivo studies revealed a 'torpedo' mechanism for Pol I termination: co-transcripti

135 subunits and for other protein components in Torpedo membrane preparations, such as RAPsyn and Na(+)-

136 roperties measured both in flux studies with Torpedo membrane vesicles and by single-channel analysis

137 ne photoaffinity labeling of both the native Torpedo membranes and the isolated nAChR was saturable,

138 Native Torpedo membranes contain approximately 35 mol % CH but

139 To seek such sites, Torpedo membranes enriched in nicotinic acetylcholine re

140 , anionic lipids that are abundant in native Torpedo membranes, also stabilize the receptor in the re

141 of ice-embedded "giant" tubular crystals of Torpedo membranes, which had been partially flattened to

142 inhibition of agonist-induced cation flux in Torpedo membranes.

143 nic acetylcholine receptor (nAChR) in native Torpedo membranes.

144 Our results suggest a unified allosteric/torpedo model in which Rat1 is not a dedicated terminati

145 A central untested feature of the torpedo model is that there is kinetic competition betwe

146 The torpedo model of transcription termination asserts that

147 The torpedo model of transcription termination by RNA polyme

148 rase II via the nascent transcript-i.e., the torpedo model.

149 scriptional termination, as envisaged in the torpedo model.

150 ion of the original cryo-electron microscopy Torpedo model; the only pentameric ligand-gated ion chan

151 is model complements the current "allosteric-torpedo" model of transcription termination, and could e

152 An integrated "allosteric-GENEi-torpedo" model that could explain this paradox predicts

153 An alternative 'torpedo' model postulated that poly(A) site cleavage pro

154 characterize mTFD-MPAB interactions with the Torpedo (muscle-type) nAChR.

155 To identify regions of the Torpedo Na,K-ATPase alpha-subunit that interact with mem

156 2)]-n-pentyldiazirine photoincorporated into Torpedo nAcChoR-rich membranes mainly in the alpha subun

157 acts as a positive allosteric potentiator of Torpedo nACh receptor (nAChR) and binds to a novel site

158 nd beta1-Cys447 in the lipid-exposed face of Torpedo nAChR alpha1M4 and beta1M4, respectively.

159 ld decrease in the apparent affinity for the Torpedo nAChR and a corresponding 150-fold increase in t

160 are the state-dependent photolabeling of the Torpedo nAChR before and after purification and reincorp

161 The Torpedo nAChR delta-subunit residue corresponding to gam

162 es of the neuronal alpha4beta2 nAChR and the Torpedo nAChR display a high degree of structural homolo

163 olabeling studies using [3H]-3-azioctanol in Torpedo nAChR identified alphaE262 as a site of desensit

164 receptor (nAChR), have been localized in the Torpedo nAChR in the desensitized state by use of a phot

165 oligand binding assays, AziPm stabilized the Torpedo nAChR in the resting state, whereas propofol sta

166 ed the binding of [(3)H]phencyclidine to the Torpedo nAChR ion channel in the resting and desensitize

167 hotoincorporated into amino acids within the Torpedo nAChR ion channel with the efficiency of photoin

168 The rat anti-Torpedo nAChR mAbs examined here are known to inhibit li

169 lpha subunit interface is a binding site for Torpedo nAChR negative allosteric modulators (TFD-etomid

170 h that interactions with gamma Trp-55 of the Torpedo nAChR play a crucial role in agonist binding and

171 of the 5-HT3A and alpha4beta2 nAChRs against Torpedo nAChR revealed MA -4', 0', and 4' residues withi

172 tein) and of the electron-microscopy-derived Torpedo nAChR structure.

173 Our data indicate that the Torpedo nAChR transmembrane domain structure is a better

174 itization in the transmembrane domain of the Torpedo nAChR using time-resolved photolabeling with the

175 With the recent 4 A resolution of the Torpedo nAChR, and the crystal structure of the AChBP, m

176 With the recent 4 A resolution of the Torpedo nAChR, and the crystal structure of the related

177 For Torpedo nAChR, photoaffinity-labeling studies with the c

178 after purification and reconstitution of the Torpedo nAChR, the difference in structure between the r

179 interaction of a long chain alcohol with the Torpedo nAChR, we have used the photoactivatible alcohol

180 [(3)H]tetracaine and [(3)H]phencyclidine to Torpedo nAChR-rich membranes (IC(50) values of 0.8 mm).

181 annel and the lipid-protein interface of the Torpedo nAChR.

182 8-11, and 13-14 are essential for binding to Torpedo nAChR.

183 alent to the surface exposed to lipid in the Torpedo nAChR.

184 packed than the corresponding region of the Torpedo nAChR.

185 e receptor (GlyR), based on the structure of Torpedo nAChR.

186 hat of acetylcholine for embryonic mouse and Torpedo nAChRs expressed in Xenopus oocytes, respectivel

187 ontribute to the ACh binding sites in mutant Torpedo nAChRs expressed in Xenopus oocytes.

188 he interactions of agonists and antagonists, Torpedo nAChRs were expressed in Xenopus oocytes, and eq

189 uilibrium binding of ion channel blockers to Torpedo nAChRs with higher affinity in the nAChR desensi

190 scale of a rapid phase of TID inhibition in Torpedo nAChRs, suggesting the formation of a transient

191 were tested for inhibitory activity against Torpedo nAChRs.

192 e and frequency dependencies of MEPPs at the Torpedo nerve-electrocyte junction are best described by

193 GABA receptors were constructed based on the torpedo neuromuscular-like nicotinic receptor structure.

194 e of the alphaM3 transmembrane domain of the Torpedo nicotinic acetylcholine receptor (AChR) was char

195 peted with alpha-bungarotoxin for binding to Torpedo nicotinic acetylcholine receptor (nAChR) (IC(50)

196 ue Thr(422) at the lipid-exposed face of the Torpedo nicotinic acetylcholine receptor (nAChR) alpha1M

197 The Torpedo nicotinic acetylcholine receptor (nAChR) is the

198 Although the Torpedo nicotinic acetylcholine receptor (nAChR) reconst

199 acaine is a noncompetitive antagonist of the Torpedo nicotinic acetylcholine receptor (nAChR) that bi

200 C) is a potent competitive antagonist of the Torpedo nicotinic acetylcholine receptor (nAChR) that bi

201 Interactions of benzophenone (BP) with the Torpedo nicotinic acetylcholine receptor (nAChR) were ch

202 Photoaffinity labeling of the Torpedo nicotinic acetylcholine receptor (nAChR) with [3

203 rientation compatible with activation of the Torpedo nicotinic acetylcholine receptor (nAChR), we use

204 ster within the agonist binding sites of the Torpedo nicotinic acetylcholine receptor (nAChR).

205 synthesized as a photoaffinity probe for the Torpedo nicotinic acetylcholine receptor (nAChR).

206 atic amine noncompetitive antagonists in the Torpedo nicotinic acetylcholine receptor (nAChR).

207 er peptide, spanning residues 181-198 of the Torpedo nicotinic acetylcholine receptor alpha1 subunit,

208 id agonist [(3)H]arecolone methiodide to the Torpedo nicotinic acetylcholine receptor has been correl

209 ignificantly, ultrastructural studies of the Torpedo nicotinic acetylcholine receptor indicate that t

210 ls of the open state of the heteropentameric Torpedo nicotinic acetylcholine receptor pore domain are

211 ting into some, but not all, subunits of the Torpedo nicotinic acetylcholine receptor to a degree tha

212 (+)-epibatidine, and (+/-)-epibatidine, with Torpedo nicotinic acetylcholine receptor-enriched membra

213 [(3)H]Azietomidate photoincorporated into Torpedo nicotinic acetylcholine receptor-rich membranes.

214 nferred from the structure of the homologous Torpedo nicotinic acetylcholine receptor.

215 mine neurotoxins tightly bound to the coated Torpedo nicotinic receptor were eluted with methanol, an

216 t biotinylated-alpha-bungarotoxin binding to Torpedo-nicotinic acetylcholine receptors in a concentra

217 tps1 embryos do not develop past late torpedo or early cotyledon stage.

218 d shown by peptide microsequencing to be the Torpedo ortholog of the small leucine-rich repeat chondr

219 estored by the introduction of rat MuSK or a Torpedo orthologue.

220 hape [thickened axonal profiles (P = 0.006), torpedoes (P = 0.038)] and changes in axonal connectivit

221 ere were approximately 7x more Purkinje cell torpedoes per section (12.6 +/- 7.9 versus 1.7 +/- 1.4,

222 tron tomography and subtomogram averaging of Torpedo postsynaptic membrane that receptors are connect

223 y electron microscopy of tubular crystals of Torpedo postsynaptic membranes embedded in amorphous ice

224 Mutations of this glutamate residue in Torpedo ray ClC channels alter gating in electrophysiolo

225 ic receptors using a structural model of the Torpedo receptor at 4 A resolution, recordings of curren

226 ctron microscopic study of the non-activated Torpedo receptor had suggested that these sites might be

227 enesis, spatially restricted activity of the TORPEDO receptor tyrosine kinase first recruits follicle

228 tudied further by single-channel analysis of Torpedo receptors reconstituted in giant liposomes.

229 F, corresponding to positions 290 and 331 in Torpedo) rendered the enzyme 10-fold less sensitive to e

230 Catch and consumption of torpedo scad (Megalaspis cordyla) over the western India

231 g information on the population structure of torpedo scad stocks it is crucial to provide population

232 2 seeds were nonviable and developed only to torpedo-shaped embryos, indicative of arrested seed embr

233 One set belongs to the gurken-torpedo signaling pathway and affects the development of

234 are noncompetitive antagonists (NCAs) of the Torpedo species nicotinic acetylcholine receptor (nAChR)

235 was observed in the embryo from seeds at the torpedo stage and later, in seedling, leaf, stem, and ro

236 detected in the endosperm from seeds at the torpedo stage and later.

237 d throughout the root epidermal layer in the torpedo stage embryo when the cell-specific pattern of G

238 tion undergoes a marked reduction at the mid-torpedo stage of Arabidopsis embryogenesis.

239 embryos that were arrested at or before the torpedo stage of development.

240 traffic 10-kD fluorescent dextran in the mid-torpedo stage of development.

241 In the torpedo stage of embryo development, ATML1 mRNA disappea

242 ze exclusion limit was found to occur at the torpedo stage of embryogenesis in Arabidopsis; at this t

243 pproximately 1/4 of which aborted before the torpedo stage, suggesting that fab1-2 represents a compl

244 embryo within them was arrested at the heart-torpedo stage.

245 ed with the activities of WER and CPC during torpedo stage.

246 , that maintain dilated plasmodesmata at the torpedo stage.

247 gy and carbon metabolism and abortion at the torpedo stage.

248 gene expression to a profile resembling late-torpedo-stage embryogenesis.

249 trastructure of PD in early-, mid-, and late-torpedo-stage embryos and in young leaves.

250 embryo development arrested from globular to torpedo stages.

251 half that of the wild type at the heart and torpedo stages.

252 chick cerebellum, chick ciliary ganglia, and Torpedo synaptic vesicles.

253 Following immunization with Torpedo (t) AChR, the IL-4(-/-) mice readily developed s

254 to track in a 5'-3' direction like a guided torpedo that ultimately helps dissociate the RNA polymer

255 te with Xrn2, the nuclear 5'-3' exonuclease "torpedo" that facilitates transcription termination at t

256 nt in cesa9 embryos, visually inspected from torpedo to bent cotyledon, consistent with no reduction

257 gurken (grk), a TGFalpha-like protein, with torpedo (top), the Drosophila EGF receptor (Egfr).

258 tly seen on the axons of Purkinje cells with torpedoes versus Purkinje cells without torpedoes.

259 On the basis of Torpedo vesicle studies, TID is thought to selectively i

260 Torpedo vesicles are negative for the sarcoplasmic/ endo

261 vesicles isolated from the electric organ of Torpedo were determined using a pH-jump protocol.

262 ar TBP-positive immunoreactivity, and axonal torpedos were readily found by light and electron micros

263 nhibition of the macroscopic response of the Torpedo wild type of about 52%, whereas the alpha C418W