ライフサイエンスコーパス: Tris

1 Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) has been

2 Tris(1,3-dichloro-2-propyl)phosphate (TDCIPP) is a high-

3 Tris(1,3-dichloroisopropyl)phosphate (TDCIPP) was the mo

4 Tris(2,2'-bipyridyl)ruthenium can be excited to fluoresc

5 Tris(2-aminoethylamine) and cis-1,3,5-tris(aminomethyl)c

6 Tris(2-benzimidazolylmethyl)amines have been found to be

7 Tris(2-butoxylethyl) phosphate (TBOEP) accounted for 40%

8 Tris(2-carboxyethyl)phosphine (TCEP) is a widely used su

9 Tris(2-pyridylthio)methane, [Tptm]H, has been employed t

10 [Tris(2-pyridylthio)methyl]zinc fluoride, [kappa(4)-Tptm]

11 [Tris(2-pyridylthio)methyl]zinc hydride, [kappa(3)-Tptm]Z

12 Tris(8-quinolinolato)gallium(III) (1, KP46) is a very pr

13 Tris(chloropropyl) phosphate isomers (SigmaTCPP) account

14 Tris(heterocyclemethyl)amines containing mixtures of 1,2

15 Tris(ligand) iron(III) complexes were prepared and inves

16 Tris(pentafluorophenyl)boron B(C6F5)3 is an effective ca

17 Tris(phenylthio)benzene molecules have been synthesized

18 Tris(phosphine)borane ligated Fe(I) centers featuring N(

19 Tris(quinolinolate)aluminum(III) (AlQ3) is the most wide

20 Tris(silyl) calixarene 7 was assigned the flattened cone

21 Tris(trialkylsilyl)silyl groups are easily prepared and

22 Tris(trifluoropropyl)trimethyl-cyclotrisiloxane and tetr

23 Tris(trimethylsilyl)amine is formed with an initial turn

24 Tris(trimethylsilyl)sulfonium 1 and methylbis(trimethyls

25 Tris(triphenylphosphinegold) oxonium tetrafluoroborate,

26 Tris-(1-chloro-2-propyl)phosphate (TCPP) was the most ab

27 Tris-arenes based on either isophthalic acid or 2,6-dipi

28 Tris-based buffers, even in optimal form, create a runaw

29 Tris-borate has a high-buffering capacity and is therefo

30 Tris-hydroxymethyl aminomethane (THAM) may be an effecti

31 ine (PBS) buffers was similar, but in pH 8.0 Tris the hydrolysis rate nearly doubled.

32 ) M p-nitrophenyl phosphate in 1.0 M, pH 9.0 Tris buffer as the substrate for the enzymatic reaction,

33 nd an s of 5 bp were obtained in 5 mM pH 7.1 Tris-HCl buffer solution containing 50 mM NaCl.

34 the melting temperatures suggests that 0.15 Tris+ ion per phosphate is released upon denaturation.

35 d optimum response within 2s at pH 8.5 (0.1M Tris-HCl) and 35 degrees C, when operated at 20 mV s(-1)

36 A low-ionic-strength solution, 6.1muS/cm 1mM Tris (pH 9.3), was used to produce ACEO and proved the f

37 CEO driving and DNA hybridization in the 1mM Tris solution were 1.5 Vpp and 200Hz.

38 n the singly reduced form, BV*+, in a pH 7.4 Tris-HCl buffer solution at mu = 8.4 mM.

39 2,4,6-Tris(chlorosulfonyl)aniline (47) was obtained by the chl

40 2,4,6-Tris(dimethylamino)-1,3,5-triazine was prepared to compl

41 MAC was achieved with crude extract at pH 7, Tris-HCl binding buffer at pH 7 and the use of 300 mM im

42 nt of a 1,4,7,10-tetraazacyclododecane-1,4,7-Tris-acetic acid ligand to bind (64)Cu(2+).

43 ffers/pH (potassium phosphate buffer pH 6-8, Tris buffer pH 8-10) on the current responses generated

44 A Tris-Acetate-Phosphate-Pluronic (TAPP) medium that under

45 cally injected into a capillary containing a Tris-based aqueous buffer.

46 iding x-ray characterization of As(III) in a Tris thiolate protein environment and allowing a structu

47 hydroxide by the bidentate coordination of a Tris molecule.

48 on was also carried out into the effect of a Tris-HCl buffer containing the surfactant Tween 20 to ai

49 igher separation resolution as compared to a Tris glycine system, destacking of proteins down to 6.5

50 uidic Western blot assay (muWestern) using a Tris tricine discontinuous buffer system suitable for an

51 mM), is separated using such a column with a Tris buffer.

52 ion (>200 mM) of the chemical reducing agent Tris(2-carboxyethyl)phosphine (TCEP) as its reduction ra

53 since the disulfide-specific reducing agent Tris(2-carboxyethyl)phosphine hydrochloride failed to su

54 odiglycol) or the non-sulphur reducing agent Tris-(2-carboxylethyl)phosphine (TCEP).

55 ther confirmed by its resistance to alkaline Tris.HCl.

56 presence of tris(hydroxymethyl)aminomethane (Tris).

57 ct is strongly ion-specific, with Ca(2+) and Tris, respectively, promoting and reducing stress-induce

58 Li(+), NH(4)(+), and Tris(+) ions bind to A-tracts with similar affinities; t

59 s solutions containing NaCl, KCl, CaCl2, and Tris buffer show that the magnitude of the effect is str

60 e presence of Na(+), K(+), Li(+), Ch(+), and Tris(+) and that the catalytic efficiency of prothrombin

61 A-binding activity, while cytochalasin D and Tris decreased both F-actin and NPA-binding activity in

62 sponses by the reductants dithiothreitol and Tris(2-carboxyethyl)phosphine (TCEP).

63 as been measured in diethylmalonate (DM) and Tris-acetate buffers, with and without added NaCl or Tri

64 red by brief illumination of O2 evolving and Tris-washed preparations at 200 K or by a single saturat

65 Similar glycerol and Tris derivatives as well as dNMPs were also formed with

66 In the presence of dATP, glycerol, and Tris buffer, the DNA primase isolated from Thermococcus

67 However, Hepes and Tris buffers did not allow formation of NO-Fe(DTCS)2.

68 Added Hepes and Tris maintained all assigned pHs.

69 ed that comigrates on reverse phase HPLC and Tris-tricine electrophoresis.

70 rs of hKAT I: indole-3-acetic acid (IAC) and Tris.

71 y(ethylene glycol) acrylate/methacrylate and Tris-HCl buffer was loaded into the microdevice.

72 chelators 2-mercaptopropionic acid (MPA) and Tris-EDTA (TE) to detect MBLs.

73 Phosphate and Tris-HCl buffers decrease the signal intensity measured

74 ox species [Ru(NH3)6](3+/2+) for pumping and Tris buffer, was transported by redox-MHD and detected w

75 e membrane (CEM) -based anion suppressor and Tris and ethylenediamine buffers using an anion-exchange

76 ed (bis-Tris, lutidine, triethanolamine, and Tris), the decomposition of PM (with and without GSH) ga

77 prevented by anti-AbetaP antibody, zinc, and Tris, but not by tachykinin neuropeptides.

78 f a sample that contains a weak base such as Tris (pK(a) = 8.2) is presented here for the first time.

79 effects of common buffer components, such as Tris and glycerol, on the biotinylation process.

80 is mediated by zinc-chelating agents such as Tris buffer, citrate, or glutathione.

81 Because Tris is able to occupy the substrate binding position, w

82 acid (0.1 N HCl), 0.1% SDS, 1 M benzamidine, Tris-HCl (1 M, pH 12), or DTT (5 mM), but it was washed

83 The structurally biomimetic Tris-imidazole model is the most selective.

84 Commercial Bis-Tris-Mes gels provide a sample reduction buffer at pH 8.

85 219 +/- 9.7 mumol/min/mg by using HEPES-Bis-Tris propane, pH 7.5.

86 V) bis-Tris propane (1.0014) and Eu(III) bis-Tris propane (1.0012).

87 iethanolamine/Tricine, pH 7.9; and (iii) Bis-Tris/Aces, pH 6.7.

88 The preparation of cell-free extract in Bis-Tris propane/HCl, pH 7, buffer containing 0.025% (w/v) T

89 Rates are significantly lower in Bis-Tris-Mes gels than in Tris-glycine gels, reducing the ri

90 oester p-nitrophenyl phosphate by Ce(IV) bis-Tris propane (1.0014) and Eu(III) bis-Tris propane (1.00

91 fect on the reaction catalyzed by Ce(IV) bis-Tris propane solutions at pH 8 was determined to be 1.00

92 xidized to Fe3+ at 37 degrees C in 20 mM Bis-Tris buffer at pH 5.8, was significantly slowed in the p

93 in the presence of 100 mM KCl and 10 mM Bis-Tris propane over a pH range of 6.00-8.00.

94 conditions employed in this study (10 mM Bis-Tris, 200 mM KCl, 2.5 mM MgCl(2), 1 mM CaCl(2), 100 micr

95 The concentration of bis-Tris buffer impacts the extent of coupling of oxygen act

96 In every buffer studied (bis-Tris, lutidine, triethanolamine, and Tris), the decompos

97 is determined to be 9.5, in contrast to Bis-Tris-Mes gels where the pH is 7.2.

98 chemical nature and concentration: Tris, bis-Tris propane, Tes, Hepes, and cacodylate.

99 The evidence includes structures with Bis-Tris (2,2-bis(hydroxymethyl)-2,2',2''-nitrilotriethanol)

100 e preparation at the higher pH used with Bis-Tris gels.

101 the homes, we detected TDBPP, or brominated "Tris," which was banned in children's sleepwear because

102 ansition Tg in a freeze-concentrated buffer (Tris-HCl).

103 m formate) with a near-physiological buffer (Tris-acetate) for three protein-ligand pairs.

104 y unfavorable interactions between the bulky Tris-borate ion and RNA or partial coordination of RNA f

105 hKAT-I is inhibited by Tris under physiological pH conditions, which explains w

106 nzymes by their sensitivity to inhibition by Tris and free arginine.

107 carried out by varying the pH maintained by Tris-HCl or CAPS buffer (pH 8.0 and 10.3) and keeping th

108 and is inhibited in a competitive manner by Tris buffer.

109 After disruption of the outer membrane by Tris-EDTA-lysozyme, the inner-membrane-anchored proteins

110 hen part of the external Na+ was replaced by Tris.

111 Hence, positively charged Tris buffer ions will compete with other monovalent cati

112 r testing by quantitative PCR were compared: Tris-EDTA (TE) buffer, PrepMan Ultra, 2% sodium dodecyl

113 e OH- ions and the cationic buffer component Tris+ results in a zone of lower conductivity, within wh

114 different chemical nature and concentration: Tris, bis-Tris propane, Tes, Hepes, and cacodylate.

115 Under some conditions, Tris-borate is a poor counterion for RNA and its use mer

116 t mixture, buffered at pH 7.8 and containing Tris, Triton X-100, glucose-6-phosphate, nicotinamide ad

117 conditions (conventional buffer: containing Tris and higher Mg2+ concentration [10-15 mM]; and polya

118 ll as otherwise identical buffers containing Tris.

119 The pH of conventional Tris-glycine SDS-PAGE gels during a run is determined to

120 esonance imaging (MRI) contrast agent, CREKA-Tris(Gd-DOTA)3 (Gd-DOTA (4,7,10-tris(carboxymethyl)-1,4,

121 Here we assess the capability of CREKA-Tris(Gd-DOTA)3 to detect micrometastasis with MRI in co-

122 We find that CREKA-Tris(Gd-DOTA)3 provides robust contrast enhancement in t

123 ts demonstrate that molecular MRI with CREKA-Tris(Gd-DOTA)3 may facilitate early detection of high-ri

124 Synthesis of the sterically crowded Tris(pentamethylcyclopentadienyl) lanthanide complexes,

125 at were identified as dAMP-glycerol and dAMP-Tris.

126 Small isolated protofibrils in dilute Tris-HCl buffers were directed along the elongation path

127 he disulfide-reducing agents dithiothreitol, Tris(2-carboxyethyl)phosphine (TCEP), or reduced glutath

128 aks in 20-80 mM TAE (TAE, Tris-acetate-EDTA; Tris, tris[hydroxymethyl]aminomethane) buffer.

129 ly performed using buffers containing either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA

130 roxylations of benzyl alcohol, ethylbenzene, Tris buffer, lauric acid, and methyl laurate and epoxida

131 High-performance explosives: Tris(triazolo)benzene was synthesized and converted to i

132 tial and temporal distribution are given for Tris-(1-chloro-2-propyl) phosphate (TCiPP), EHDPP, tri-n

133 ase pair (bp) oligomer ranged from 71 mM for Tris+ to 173 mM for Na+ and K+.

134 .3 A X-ray structure of the GDPMH-Mg(2+)-GDP.Tris(+) complex, the GDP leaving group interacts with fi

135 was permeable to Na(+) approximately K(+) >> Tris(+), and whole-cell current density at -80 mV increa

136 action procedures, including the use of HCl, Tris-HCl buffer, and enzymatic hydrolysis (using protein

137 examined (substituted imidazoles, histidine, Tris, and 1,4-diazabicyclo[2.2.2]octane).

138 gainst the N-terminal region of AbetaP, (ii) Tris, and (iii) Zn2+.

139 with o-phenanthroline and eosin at pH 7.5 in Tris; a piece of filter paper was used as a solid suppor

140 ive E. coli O157:H7 bacteria was achieved in Tris-buffered saline in an assay time of ca. 45 or ca. 3

141 orm of holoShp (hemoShp) is stable in air in Tris-HCl buffer, pH 8.0, binds to apoHtsA with a K(d) of

142 A and PNA (peptide nucleic acid) beacons, in Tris-buffer solutions containing various concentrations

143 fragments ranging from 2,027 to 23,130 bp in Tris-borate-EDTA buffer solutions was investigated.

144 At 25 degrees C in Tris at pH 8.3, MutL assembles into a heterogeneous mixt

145 ill compete with other monovalent cations in Tris-buffered solutions.

146 validated by correctly sensing pH changes in Tris and acetate solutions.

147 In contrast, in Tris-borate, RNA collapse has a much smaller apparent Mg

148 en 20) and Cetylpyridinium chloride (CPC) in Tris/HCl buffer, pH 8.5.

149 een VAL and Li(+), Na(+), K(+), and Cs(+) in Tris buffer solution were determined to be 67+/-42, 120+

150 face charge density were subjected to CZE in Tris-HCl (pH 8) buffers of various ionic strengths (0.00

151 een measured by capillary electrophoresis in Tris-acetate buffer, to test the hypothesis that site-sp

152 er nucleotides increase the rate >20-fold in Tris buffer.

153 Msp(Fl) folded in Tris buffer contained slightly less beta-sheet structure

154 glass surfaces, RCA-cleaned and immersed in Tris-EDTA buffer were also studied.

155 ncated at D421 was detected at low levels in Tris-soluble and detergent-soluble tau at 3 to 6 months

156 zine synthase with a Ki of 66+/-13 microM in Tris buffer and 22+/-4 microM in phosphate buffer.

157 one-4-phosphate substrate from 5.2 microM in Tris buffer or from 6.7 microM in MOPS buffer to 50 micr

158 trypsin ranging from 0.002 to 2 microg/ml in Tris-buffered saline buffer for 2 h.

159 egrees C and [Mg(2+)] approximately 10 mM in Tris-borate (TB) buffer.

160 ic strengths, it could be easily observed in Tris buffer, containing 150 mM NaCl.

161 ay conditions, the structure was obtained in Tris buffer at pH 6.8 and without the presence of organi

162 oximately 3.75 wt % linear polyacrylamide in Tris-HCl) is used to obtain fully stretched configuratio

163 roximately 10 days), second-order process in Tris-buffered saline.

164 e containing the dipeptide glycyl-proline in Tris-HCl supplemented with manganese at 37 degrees C for

165 of inhibition and fluorescence quenching in Tris buffer occurs when an average of two serine binding

166 Screening of selenium in Tris-buffer-urea soluble fraction was carried out by sod

167 homogenization of the excised gel slice (in Tris-EDTA buffer), containing the DNA fragment of intere

168 cores, the Tg mice peptides were soluble in Tris-SDS-EDTA solutions, revealing both monomeric and SD

169 ) were used to characterize PHF suspended in Tris-buffered saline (TBS), sodium acetate buffer, and w

170 tidyl choline (DOPC) liposomal suspension in Tris-HCl buffer (pH 7.4 at 40 degrees C) has been develo

171 on of the rac-alcohols using W110A TESADH in Tris buffer/acetone (90:10, v/v).

172 moanaerobacter ethanolicus (W110A TESADH) in Tris buffer using 2-propanol (30%, v/v) as cosolvent and

173 However, when tested in Tris buffer vs Mycobacterium tuberculosis lumazine synth

174 ificantly lower in Bis-Tris-Mes gels than in Tris-glycine gels, reducing the risk of adventitious pro

175 fractional site occupancy indicates that in Tris buffer, one serine is bound to each interface at ma

176 We also show that in Tris-buffered solutions the Raman signature of supercoil

177 ay involves homogenization of the tissues in Tris-HCl:glycerol buffer and the recording of Soret exci

178 different DNA extraction methods, including Tris-EDTA Method, a modified Cetyltrimethylammonium Brom

179 correlated with (1) significantly increased Tris-buffered saline (TBS)-insoluble Abeta(42) levels an

180 nto random-coil structure upon dilution into Tris buffer.

181 ement over earlier model reactions involving Tris.

182 ay 3), injected CA II protein + Tris or just Tris (Day 3), measured I(NBC) or the initial rate at whi

183 d out in buffered medium (methanol: 10mmol/l Tris buffer pH 7.5, 1:1 v/v) and reaction was completed

184 In a solution containing 0.04 M Tris-acetate buffer at 25 degrees C, calculated mobiliti

185 is routinely stored at 4 degreesC, in 0.05 m Tris/HCl buffer containing 25% glycerol and at high prot

186 l(2) and 2.5 mM Ru(NH(3))(6) Cl(3), in 0.1 M Tris buffer (pH = 9).

187 In addition, the same measurements in 0.1 M Tris buffer at pH 7.0 show a much slower exchange rate f

188 ine derivatives were well separated in 0.1 M Tris-0.1 M borate-2 mM EDTA buffer (pH 8.75) using a 60-

189 rom the Golgi membrane by treatment with 1 M Tris-HCl (pH 7) or upon reincubation at 37 degreesC, whe

190 when operated at 0.2V (vs. Ag/AgCl) in 0.1 M Tris-HCl buffer, pH 8.5 and at 35 degrees C.

191 ly resistant to chemical extraction with 1 M Tris-HCl, pH 7, indicating that the adaptor binds to bot

192 l peptides at pH 7.4, 37.0 degrees C, 0.15 M Tris.HCl buffer have been determined; a library of 913 a

193 In 0.20 M Tris buffer solution (pH 7.4), an irreversible, overlapp

194 s without an aminosulfonate moiety (maleate, Tris, and bicarbonate).

195 in the presence of NRS in a buffered medium (Tris; pH 8.0) with formation of Fe(II)/NRS complexes.

196 , 27% sucrose (wt/vol), 2 mM EDTA, and 10 mM Tris (pH 9), were required for efficient OM release, as

197 propylamine and O(2) as substrates in 10 mM Tris HCl, pH 7.9, 4 degrees C.

198 f 2400(+/-600) nt before dissociation (10 mM Tris-HCl (pH 8.3), 20 mM NaCl, 20% (v/v) glycerol, 25 de

199 potassium glutamate and 8 mM MgCl2 or 10 mM Tris-HCl and 200 mM KCl, with or without 0.5% Tween adde

200 erved with an assay mixture containing 15 mM Tris-HCl buffer at neutral pH (6.5-8.5) and at 37 degree

201 e addition of glycerol to 500 mM NaCl, 20 mM Tris (pH 8.4), 2 mM beta-mercaptoethanol significantly e

202 by sodium borohydride (200 microm) in 20 mm Tris-HCl, 1 mm EDTA at 37 degrees C, pH 7.4, gives a 50-

203 were completed in less than 30 s using 25 mM Tris, 192 mM glycine at pH 8.5 as the electrophoresis bu

204 formed directly from a 150 mM KCl and 25 mM Tris-HCl buffer at pH 7 that is widely used in protein c

205 n even in the presence of 0.05% SDS in 25 mM Tris/HCl buffer, which indicated that it was highly asso

206 onditions [350 mM KCl, 8 mM MgCl2, and 30 mM Tris (pH 7.5)], a complex with an association constant o

207 e of 1.27 +/- 0.06 kcal mol(-1) M(-1) (30 mM Tris-HCl, pH 8, 1 mM DTT, 25 degrees C).

208 ation constant in our standard buffer (40 mm Tris (pH 7.9), 10 mm MgCl(2), 0.1 mm dithiothreitol, 5%

209 s were measured for eight oligomers in 40 mM Tris acetate buffer.

210 icrom C(18) packing with 75% ACN (v/v), 5 mM Tris at pH 8.0.

211 r Eu(III) (6.2 +/- 0.3 microM) (pH 7.8, 5 mM Tris) were determined by tryptophan fluorescence titrati

212 ere mobile phase containing 90/10 ACN/2.5 mM Tris, pH 8, sheath liquid containing 50/50 MeOH/10 mM HC

213 om virions disrupted by treatment with 50 mM Tris (pH 7.5), 0.5 M NaCl, 0.5% NP-40, and 10 mM dithiot

214 that Ms-Lon associates to a hexamer at 50 mM Tris and 10 mM MgCl(2), at pH 8.0 and 20 degrees C, and

215 ormation constants (in the presence of 50 mM Tris) of P3W for Eu(III) (K(a) = (2.1 +/- 0.1) x 10(5) M

216 sieving ability at 0.5% (w/w) in TTE (50 mM Tris, 50 mM TAPS, 2 mM EDTA, pH 8.4) buffer.

217 odiscs at 20 degrees C in 100 mM NaCl, 50 mM Tris, at pH 7.4.

218 electrolytes (BGEs) (100 mM H(3)PO(4), 50 mM Tris, pH 2.25; 500 mM acetic acid, pH 2.54; 200 mM formi

219 llel quadruplex structure in 22AG-ThT (50 mM Tris, pH 7.2) solution to the antiparallel form just by

220 nd their extension rates determined in 50 mM Tris, pH 8.3, 0.5 mg/ml BSA, 2 mM MgCl(2), and 200 muM e

221 nditions of ionic strength (163 mM) in 50 mM Tris-HCl (pH 7.4) at 37 degrees C.

222 Na(2)S, and 40 microM holo frataxin in 50 mM Tris-HCl (pH 7.5) with 4.3 mM DTT.

223 irpins (and, presumably, duplexes) bind more Tris+ ions than Na+ ions in solution.

224 tides can be modified to the corresponding N-Tris(2, 4,6-trimethoxyphenyl)phosphonium-acetyl (TMPP-Ac

225 after 6.25 h of hybridization in 50 mM NaCl Tris buffer.

226 bovine serum albumin, washing with EDTA/NaCl/Tris buffer, and spraying with inert gases, were used to

227 Upon nonreducing Tris-Tricine-urea-SDS-PAGE, newly synthesized proinsulin

228 ere performed using 2g of sample and 20ml of Tris-HNO3 (pH=8) containing: a) 0.1M NaCl and 2g of skim

229 at pH 8.5 versus the conventional pH 6.8 of Tris-glycine gels.

230 ric DNA, both in the presence and absence of Tris buffer/salt, and sensing the same through its fluor

231 n APP at the mRNA level affect the amount of Tris buffer extractable APP protein and Abeta40 and 42 p

232 coiled DNA can be obscured by Raman bands of Tris counterions.

233 The binding of Tris+, NH4+, Li+, Na+, and K+ to dsDNA was then investig

234 The weak capacity of Tris-borate to stabilize RNA folding may reflect relativ

235 Using various relative concentrations of Tris and either phosphate, tricarballylate (1,2,3-propan

236 red lysis buffer with high concentrations of Tris-HCl and sodium dodecyl sulfate as well as exposure

237 act in vitro with an oxidative derivative of Tris, resulting in strand cleavage.

238 on was between 8.5 and 8.7 with no effect of Tris concentration.

239 However, the effectiveness of Tris-borate as a counterion for RNA has not been systema

240 The use of volatile buffers instead of Tris-acetate in detection of small molecules by MS impro

241 atic digestions of the substrate in 50 mM of Tris buffer at pH 7.4 generates fluorescence emission at

242 Because the apparent number of Tris+ ions released is greater than that observed by oth

243 red in 90% yields by the Mannich reaction of Tris(hydroxymethyl)phosphine 1 with (l)- or (d)- Alanine

244 On the other hand, the use of Tris-HCl as binding buffer gave higher purification perf

245 nds to four Mn2+ bound with high affinity on Tris-washed photosystem II (PSII) membrane fragments.

246 tion is a hydrophilic ion like Na+, NH4+, or Tris+ or a hydrophobic ion like tetrabutylammonium.

247 ution buffered to pH 8-8.2 with phosphate or Tris-HCl, followed by reaction with TMPP-acetic acid N-h

248 ning either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA (TBE).

249 lted in greater sensitivity than with TBS or Tris.

250 0 min exposure, a fully assembled (mu(3)-oxo)Tris[(mu(2)-peroxo)(mu(2)-glutamato-kappaO:kappaO')](glu

251 of hKAT-I and reassesses the effects of pH, Tris, amino acids and alpha-keto acids on the activity o

252 ions for monovalent ions (sodium, potassium, Tris), magnesium ions and commonly used denaturing agent

253 r 2-amino-2-(hydroxymethyl)-1,3-propanediol (Tris base).

254 tage clamp (Day 3), injected CA II protein + Tris or just Tris (Day 3), measured I(NBC) or the initia

255 ssed the utility of the sulfhydryl reductant Tris(2-carboxyethyl)phosphine (TCEP) for both nucleic ac

256 dine hydrochloride (GdnHCl) or the reductant Tris(2-carboxyethyl)phosphine (TCEP) has been analyzed u

257 K, L, and S at neutral pH by high resolution Tris-Tricine SDS-PAGE and matrix-assisted laser desorpti

258 extraction buffers (phosphate buffer saline, Tris-HCl and NaCl) on the extraction efficiency of total

259 Each amphibactin has the same Tris-hydroxamate-containing peptidic headgroup composed

260 freezing without cryoprotection in a simple Tris.HCl buffer containing EGTA (50 mM) and NaCl (50 mM)

261 the effects of magnesium, potassium, sodium, Tris ions, and deoxynucleoside triphosphates on melting

262 e under stirring whereas under non-stirring, Tris buffer at pH 10 with 0.03M (NH4)2SO4 and 30microm M

263 lution of the DNA using a low ionic strength Tris buffer at pH 8.

264 teractions are present in low ionic strength Tris buffers when vimentin is maintained at the "protofi

265 dynorphin A or somatostatin 28 as substrate, Tris caused a decrease in KM of up to 100 fold, again wi

266 neoendorphin, and dynorphin B as substrates, Tris increased KM up to 40-fold with little change in Vm

267 honate and alpha-carboxylate, we synthesized Tris-POC-2-PMPA (21b), which afforded excellent release

268 15-308.15 K without preparation of synthetic Tris seawater buffers.

269 ntration than is possible with standard TAE (Tris/acetate, pH 8.0) or TBE (Tris/borate, pH 8.3) buffe

270 Gaussian-shaped peaks in 20-80 mM TAE (TAE, Tris-acetate-EDTA; Tris, tris[hydroxymethyl]aminomethane

271 form complexes with the borate ions in TBE (Tris-borate-EDTA) buffer, with mobilities that depend on

272 standard TAE (Tris/acetate, pH 8.0) or TBE (Tris/borate, pH 8.3) buffers.

273 Because dpH(i)/dt was greater in CA II than Tris oocytes, and EZA eliminated the difference, injecte

274 The Tris tricine muWestern is completed in an enclosed, stra

275 n ALP was incubated with BCIP for 1 h in the Tris-HCl buffer.

276 sured for two oligomers as a function of the Tris acetate buffer concentration.

277 ept antibody probe plug delivery scheme, the Tris tricine muWestern blot enables 40% higher separatio

278 m alternative reaction pathways available to Tris.

279 times higher using 0.5M NaCl as compared to Tris-HCl and phosphate buffer saline.

280 tage incubation on ice is fully sensitive to Tris extraction, indicating that the priming stage has p

281 Because sensitivity to Tris extraction correlates well with nucleotide hydrolys

282 H)(2)(6-Me(3)-TPA)(2)](2+) (1) [6-Me(3)-TPA, Tris(6-methyl-2-pyridylmethyl)amine] with O(2) in CH(2)C

283 Golden trefoils: Tris(alkyne)gold complex [(coct)(3)Au][SbF(6)] (see pict

284 ein solutions (pH 7.4) were reduced by using Tris (2-carboxyethyl) phosphine HCl and irradiated with

285 azidolinker are efficiently removed by using Tris(2-carboxyethyl)phosphine in aqueous solution that i

286 An extraction procedure using Tris-HCl buffer solution was employed in order to extrac

287 r, EZA had identical effects in CA II versus Tris oocytes.

288 However, when Tris-boric acid-EDTA (TBE, pH 9.1) buffer was used, the

289 PI protein levels positively correlated with Tris-extractable, soluble Abeta40 (p=0.046) and 42 level

290 forms a stable adduct very efficiently with Tris, which protects the abasic site against cleavage.

291 (37.8-46.5%) bound to the column eluted with Tris-HCl buffer, pH 7.5 with a yield up to 76.6%.

292 nsistently more resistant to extraction with Tris.

293 thylene glycol ester of N-formylglycine with Tris(hydroxymethyl)aminomethane, with the rate of peptid

294 ced by replacement of extracellular Na+ with Tris, by Ni2+ or the Na+/Ca2+ exchanger blocker KB-R7943

295 Replacement of external NaCl with Tris-HCl significantly reduced the magnitude of the GRP-

296 ysis was performed by partial reduction with Tris(2-carboxyethyl)phosphine and real-time mass monitor

297 the oxidized cysteine residues in rSeBP with Tris(2-carboxyethyl)phosphine required addition of a den

298 matin and heme aggregates, sequentially with Tris/SDS buffer and alkaline bicarbonate solution for co

299 ctive media remained largely unchanged, with Tris as the primary cation.

300 hange was prevented in the presence of zinc, Tris, and anti-AbetaP antibody but not in the presence o