ライフサイエンスコーパス: U5 snRNP

1 A U5 snRNP protein, hPrp8, interacts closely with the GU d

2 ng proteins and demonstrated that PRP4K is a U5 snRNP-associated kinase.

3 ions correlated with enhanced retention of a U5 snRNP subunit on transcription complexes downstream o

4 etically exacerbated by mutations in PRP8, a U5 snRNP protein that physically interacts with Snu114,

5 we propose that dephosphorylation of U2 and U5 snRNP components by PP1/PP2A family phosphatases faci

6 plicing factors including core Sm and U2 and U5 snRNP components.

7 NAs, pre-mRNA splicing intermediates, U2 and U5 snRNP proteins, the Nineteen Complex (NTC), and secon

8 We propose that U1 and U5 snRNPs functionally collaborate to recognize and defi

9 cells contain higher levels of stable U1 and U5 snRNPs when Lhp1p is present.

10 ntary to U12 or U5 demonstrates that U12 and U5 snRNPs perform essential roles in the AT-AC spliceoso

11 l electrophoresis reveals that U11, U12, and U5 snRNPs assemble onto the P120 pre-mRNA to form splici

12 e provide evidence that components of U2 and U5 snRNPs, specifically SAP155 and U5-116 kDa, are the k

13 h the core Sm proteins of the U1, U2, U4 and U5 snRNPs.

14 The U1, U2, U4, and U5 snRNPs each contain a common set of seven Sm proteins

15 in splicing, the recruitment of U1, U4, and U5 snRNPs to transcriptional units is not affected.

16 The Sm core proteins of U1, U2, U4/U6 and U5 snRNPs include B(B1), B'(B2), N(B3), D1, D2, D3, E, F

17 al kinase that, in association with the both U5 snRNP and N-CoR deacetylase complexes, demonstrates a

18 isplacement of U1 from the 5' splice site by U5 snRNP.

19 vel mutation in the evolutionarily conserved U5 snRNP protein Prp8 suppresses the U4-cs1 growth defec

20 PRP8 encodes a highly conserved U5 snRNP protein required for spliceosome assembly and l

21 specific function to any part of the 280-kD U5 snRNP protein has been difficult, in part because Prp

22 A, the 3'-->5' helicase acts to disrupt mRNA/U5 snRNP contacts, thereby liberating the mRNA from the

23 coactivator, and hPrp8p, a core component of U5 snRNP and spliceosomes.

24 ce that all three proteins are components of U5 snRNP.

25 tem promotes the assembly of a key module of U5 snRNP while assuring the quality control of PRPF8.

26 Reconstitution of U5 snRNPs in vitro indicated that U5 loop 1-5' exon inte

27 depleted splicing extracts with reassembled U5 snRNP particles, molecular neighbors were assessed as

28 Together, these observations suggest that U5 snRNP is positioned on the 3' splice site by an inter

29 The U5 snRNP (small ribonucleoprotein) contains several func

30 d not detect Fl(2)d complexes containing the U5 snRNP protein U5-40K or with a protein that associate

31 chains increase the affinity of Prp3 for the U5 snRNP component Prp8, thereby allowing for the stabil

32 Furthermore, the U5 snRNP protein, hPrp8, did not cross-link to the NRS p

33 with RNA splicing regulators, including the U5 snRNP components of the spliceosome, such as EFTUD2.

34 acts within the spliceosome that involve the U5 snRNP.

35 g factor PRPF8 is a crucial component of the U5 snRNP, and together with EFTUD2 and SNRNP200, it form

36 ilize them, and promote the formation of the U5 snRNP.

37 Two (cwf6p and cwf10p) are members of the U5 snRNP; one (cwf9p) is a core snRNP protein.

38 in turn, by its location with respect to the U5 snRNP binding site.

39 rr2, a pivotal spliceosomal helicase, to the U5 snRNP.

40 igmentosa assemble less efficiently with the U5 snRNP and bind more strongly to R2TP, with one mutant

41 of PRP31 regulates its interaction with the U5 snRNP component PRP8, which is required for the effic

42 , mediated through its interactions with the U5 snRNP.

43 The Ul/U4/U5 snRNP complex interacts specifically with an RNA olig

44 s the U2/U6 interaction, and induces a Ul/U4/U5 snRNP complex.

45 The yeast U4/U6.U5 snRNP complex has been purified to near homogeneity b

46 The purification of the low abundance U4/U6.U5 snRNP from yeast and the powerful sequencing methodol

47 m proteins, eight previously described U4/U6.U5 snRNP proteins, and four novel proteins.

48 allowing for the stabilization of the U4/U6.U5 snRNP.

49 18 is a constituent of the U4/U6 and U4/U6 x U5 snRNP particles.

50 ential for the formation of U4/U6 or U4/U6 x U5 snRNPs.