ライフサイエンスコーパス: UAG

1 UAG did not alter growth hormone, cortisol, glucagon, or

2 UAG is translated as pyrrolysine with the participation

3 UAG selectively decreased glucose and fructose consumpti

4 UAG translation as pyrrolysine requires the pylT gene pr

5 UAG translation was not compromised, as recombinant MtmB

6 onserved HIV-1 3' splice sites by binding 5'-UAG-3' elements embedded within regions containing RNA s

7 n patient, 19,912 tests were ordered (12,569 UAG, 3,747 cultures, and 3,596 PCR) with 378 positive re

8 This mutant tRNA is also a UAG nonsense suppressor via first base wobble.

9 Although a UAG codon typically directs translation termination, its

10 ing gap while the rest either terminate at a UAG stop codon immediately following codon 46 or fail to

11 ct, replacement of the tnaC start codon by a UAG stop codon reduces expression significantly, suggest

12 re in the same reading frame, separated by a UAG stop codon, and termination codon readthrough is req

13 in the same reading frame but separated by a UAG stop codon, require that 5-10 % of ribosomes decode

14 mutation, sup35-2, is now shown to contain a UAG stop codon prior to the essential region of the gene

15 produced from a gene that does not contain a UAG stop codon.

16 l termination, or enhanced readthrough, of a UAG stop codon at the end of gag.

17 amino acid, is incorporated in response to a UAG amber stop codon.

18 RNAs with altered anticodons which bind to a UAG nonsense (amber) codon and to the Arg (AGG), Asn (AA

19 An RNA with a UAG loop bound with highest affinity, and chemical modif

20 ORF AUG-initiating codon was replaced with a UAG stop codon along with a U112A mutation to maintain a

21 s certain trinucleotide sequences (UAU, AAG, UAG, AAU).

22 Compared with the saline control, AG and AG+UAG both decreased AIRg, but UAG alone had no effect.

23 crog/kg/h), UAG (4 microg/kg/h), combined AG+UAG, or saline were infused to 17 healthy subjects (9 me

24 f tRNA(Ala); a G3A change in Ashbya tRNA(Ala)UAG abolishes its recognition by AgAlaRS.

25 Enzymatic studies reveal that tRNA(Ala)UAG is efficiently recognized by A. gossypii mitochondri

26 urther demonstrate that a predicted tRNA(Ala)UAG is transcribed and accurately processed in vivo, and

27 to the 70S ribosome in response to an amber (UAG) codon at 3.6-A resolution.

28 DNA template contains a complementary amber (UAG) codon instead of the normal initiation (AUG) codon.

29 ltransferases all contain an in-frame amber (UAG) codon that is read through during translation.

30 Single in-frame amber (UAG) codons are found in the genes encoding MtmB, MtbB,

31 tivity in the specific suppression of amber (UAG) and ochre (UAA) codons, respectively.

32 id, because it is encoded by a single amber (UAG) codon in methylamine methyltransferase transcripts.

33 codons scattered throughout motB with amber (UAG) codons.

34 duction of p8 from transcripts containing an UAG stop codon that blocked p21 production.

35 rrow (ibm)-infused acylated ghrelin (AG) and UAG were abolished in male GHS-R-null mice.

36 to RNA targets that contain multiple GAG and UAG repeats.

37 ontaining UAG repeats or alternating GAG and UAG repeats.

38 etic genes by binding to a series of GAG and UAG trinucleotide repeats generally separated by two or

39 were 100% for culture and 99.9% for PCR and UAG.

40 ases, the sensitivities of culture, PCR, and UAG were 50%, 92%, and 96%, respectively.

41 hile Euplotes species recognize only UAA and UAG as stop codons.

42 efficient translation termination at UAA and UAG codons but not at the UGA codon.

43 3 requirement for peptide release at UAA and UAG codons, but not UGA codons.

44 recognition of only the conventional UAA and UAG termination codons.

45 ains 2 and 3 (Eo/Sc eRF1) recognized UAA and UAG, but not UGA, as stop codons.

46 he insertion of Gln, Tyr, and Lys at UAA and UAG, whereas Trp, Arg, and Cys were inserted at UGA, and

47 Sec and Pyl are encoded by UGA and UAG codons, respectively, which normally serve as stop s

48 y second amino acids, are encoded by UGA and UAG, respectively, which are the codons that usually fun

49 ere searched for Legionella urinary antigen (UAG), culture, and PCR tests ordered from March 2010 thr

50 i translation terminates more efficiently at UAG and UAA than at UGA.

51 tion of NSAA and natural AA incorporation at UAG in a recombinant reporter protein.

52 variant [PylRS-AA]) to incorporate ncAAs at UAG codons in super-folder green fluorescence protein (s

53 fully competent to terminate translation at UAG and UAA codons, that ribosomes become less efficient

54 volving a variety of initiation codons (AUG, UAG, CAG, GUC, AUC, and UUC) provide support to the hypo

55 Both UAG and UGA are able to effectively terminate PsaB synth

56 trol, AG and AG+UAG both decreased AIRg, but UAG alone had no effect.

57 translational Sep incorporation (directed by UAG) into any desired position in a protein by an Escher

58 atural amino acid and genetically encoded by UAG, becomes attached to its cognate tRNA by pyrrolysyl-

59 larly, it is thought that Pyl is inserted by UAG codons with the help of a putative pyrrolysine inser

60 Selenoprotein synthesis programmed by UAG in Geodermatophilus and Blastococcus, and by the Cys

61 ition of expression when UGA was replaced by UAG or UAA and the appropriate suppressor was present.

62 50% when the UGA stop codon was replaced by UAG or UAA, respectively, consistent with the finding th

63 rted amino acid at the position specified by UAG is not critical, as Pyl or Trp insertion yields acti

64 to proteins from a phosphotyrosyl-tRNACUA by UAG codon suppression during in vitro translation.

65 A mechanism that circumvents UAG-directed termination of translation must operate dur

66 phenylalanine in response to the amber codon UAG.

67 code an operon that reassigns the stop codon UAG to pyrrolysine (Pyl), a genetic code variant that re

68 a, which naturally recognizes the stop codon UAG.

69 proteins in response to the amber stop codon UAG.

70 yrrolysine, encoded by the termination codon UAG.

71 yrrolysine, encoded by the termination codon UAG.

72 oration in response to the amber stop codon (UAG) in mammalian cells is commonly considered to be low

73 ncy of termination by RF1 at the stop codon (UAG).

74 ophan codon (UGG) to a premature stop codon (UAG).

75 (Sec) species that recognize the stop codons UAG and UAA, and ten sense codons.

76 lE exhibits a preference for the stop codons UAG and UGA and sense codons CAG and UCG in vitro.

77 test whether infrequently used stop codons (UAG and UGA) can terminate translation in vivo.

78 F1 recognizes each of the three stop codons (UAG, UAA, and UGA) and facilitates release of the nascen

79 res of TRAP in a complex with RNA containing UAG triplets separated by two nucleotides and in a compl

80 table than those with transcripts containing UAG repeats or alternating GAG and UAG repeats.

81 a acetivorans (DeltapylT) that cannot decode UAG as Pyl or grow on trimethylamine.

82 of a Methanosarcina species unable to decode UAG codons as pyrrolysine, but also that loss of pylT do

83 amino acid, gets acylated to its distinctive UAG suppressor tRNA(Pyl) by the cognate pyrrolysyl-tRNA

84 ired to biosynthesize and genetically encode UAG codons as Pyl, is present in the genomes of 24 anaer

85 Strains lacking L11 exhibited UAG suppression, defective growth, and high-temperature

86 Thus, the adipogenic action of exogenous UAG in tibial marrow is dependent upon acylation by GOAT

87 ly recoded strain of E. coli with a flexible UAG codon we produce site-specific serine- or phosphoser

88 0.4% for culture, 0.8% for PCR, and 2.7% for UAG.

89 We confirmed that MSI1 has a preference for UAG sequences contained in a particular structural conte

90 a recombinant system employing tRNA(Pyl) for UAG suppression.

91 regions flanking mtmB1 were not required for UAG translation, but loss of a downstream pyrrolysine in

92 presence of L11 in ribosomes is required for UAG-dependent termination and is somewhat inhibitory of

93 l examination of contextual requirements for UAG translation as pyrrolysine.

94 ely compete with translation termination for UAG codons obviating the need for a specific PYLIS struc

95 ensure efficient translation of the in-frame UAG codon in case of pyrrolysine deficiency and safeguar

96 ize Pyl have few genes that contain in-frame UAG codons, and many of these are followed with nearby U

97 in either a complete Vaa ORF or an in-frame UAG stop codon immediately downstream of the poly-A trac

98 in a position that is encoded by an in-frame UAG stop codon in the mRNA.

99 An in-frame UAG within a bacterial uidA transcript was translated in

100 Protein production from UAG-containing mRNAs was verified for 19 proteins.

101 ey are in homo-11-mers and that individual G/UAG triplets within the RNA can bind to TRAP differently

102 tryptophan-activated TRAP contain multiple G/UAG repeats and show a strong bias for pyrimidines as th

103 Unacylated ghrelin (UAG) is the predominant ghrelin isoform in the circulati

104 hrelin receptor (GHS-R), unacylated ghrelin (UAG) possesses a unique activity spectrum that includes

105 AG (1 microg/kg/h), UAG (4 microg/kg/h), combined AG+UAG, or saline were inf

106 tract partially deactivated RF1 and improved UAG codon readthrough by as much as 11-fold, as demonstr

107 high temperature, but they were defective in UAG-dependent termination.

108 st kinetic studies revealed that the gain in UAG reading by RF2 R213I is associated with a reduced ef

109 S substrate resulted in a linear increase in UAG suppression, providing a facile method to assay bioa

110 c code of E. coli can be expanded to include UAG-directed pyrrolysine incorporation into proteins.

111 inally, the adipogenic effect of ibm-infused UAG was completely abolished in GOAT-KO mice.

112 Introducing UAG or UAA stop codons rather than the normal tnaC UGA s

113 characterized the functions of AMD1 and its UAG to UGG editing event.

114 We replaced all known UAG stop codons in Escherichia coli MG1655 with synonymo

115 Extra copies of the tRNA(Leu)(UAG) gene rescued the cold sensitivity and in vitro spli

116 ethanogen monomethylamine methyltransferase, UAG was translated as pyrrolysine to produce recombinant

117 interrupted by a single in-frame, midframe, UAG codon which was also found in mtmB from M. barkeri N

118 y also extended protein synthesis at natural UAG terminated messenger RNAs.

119 We believe that the versatility of this new UAG-directed production methodology should enable many f

120 utations (cxs-5, -7 and -12) and a nonsense (UAG) codon substitution (cxs-10) in the yjbD coding sequ

121 esizing system by readthrough of a nonsense (UAG) codon with a suppressor tRNA that had been activate

122 n production from mRNAs containing nonsense (UAG) codons in the presence of misacylated suppressor tR

123 ing system via readthrough of mRNA nonsense (UAG) codons by chemically misacylated suppressor tRNAs.

124 o proteins via readthrough of mRNA nonsense (UAG) codons by chemically misacylated suppressor tRNAs.

125 Neither UAA nor UAG mutations, examined at the same codon positions, wer

126 AG, but not UAG, reduced DI and kg and increased plasma growth hormo

127 ain fatty acids utilised in the acylation of UAG, including octanoic acid.

128 to previous reports, acute administration of UAG does not have independent effects on glucose toleran

129 d 2Thf-lys supporting the highest amounts of UAG translation.

130 s indicate that in-depth genetic analysis of UAG translation as pyrrolysine is feasible, as deletion

131 e first step towards the genetic analysis of UAG translation as pyrrolysine, a 761 base-pair genomic

132 UTRs at sites enriched in multiple copies of UAG motifs in epithelial progenitor cells.

133 The decoding of UAG as pyrrolysine requires pylT, which produces tRNA(Py

134 eomic workflow that enabled the detection of UAG readthrough in native proteins in E. coli strains in

135 system with EF-Sel1 raises the efficiency of UAG-specific Sec incorporation to >90%, and also doubles

136 UAG-directed termination with enhancement of UAG translation by the PYLIS appears analogous to cis-ac

137 we re-appraised the potential interaction of UAG with GHS-R in the regulation of bone marrow adiposit

138 well as analyses of the coding potential of UAG codons, overlapping genes, and release factor sequen

139 tion of release factor 1 and reassignment of UAG translation function.

140 Redefinition of UAG, UAA and UGA to specify a standard amino acid occurs

141 in Escherichia coli enhances translation of UAG (Stop) codons, yet may also extended protein synthes

142 thyltransferases made via the translation of UAG as pyrrolysine.

143 e Pyl-tRNA(Pyl) formation and translation of UAG by transcriptional deactivation of genes in the Pyl

144 Translation of UAG codons was verified by MS/MS for eight proteins, inc

145 CUA)) and pylS results in the translation of UAG in vivo as a sense codon.

146 natural genetic codes by the translation of UAG, a canonical stop codon.

147 tryptophan-activated TRAP interacts with one UAG, one AAG, and seven GAG repeats present in the trpG

148 RNAs that contained GAG and/or UAG repeats were tested while the length and sequence of

149 work, we show that RNAs containing 11 GAG or UAG repeats separated by CC dinucleotide spacers (((G/U)

150 ut in neither case was readthrough of UAA or UAG observed.

151 adthrough, namely Gln, Lys, or Tyr at UAA or UAG PTCs and Trp, Arg, or Cys at UGA PTCs.

152 to one of the three stop codons (UGA, UAA or UAG) results in the termination of protein synthesis.

153 be efficiently incorporated at a predefined UAG amber codon, thereby competing with RF1 rather than

154 However, predominant UAG-directed termination with enhancement of UAG transla

155 ontinue with translation through a premature UAG stop codon located in a beta-galactosidase reporter.

156 nslational pause by suppressor tRNAs reading UAG at these two positions may divert the nascent polype

157 fidelity of protein synthesis at reassigned UAG codons and the purity of the NSAA containing protein

158 element driving recognition is the sequence UAG.

159 nt protein (GFP) gene that contains a single UAG stop codon at two distinct locations is introduced.

160 tiple amino acid (AA) insertions at a single UAG.

161 5-fold improved reading of the RF1-specific UAG codon relative to UAA, the universal stop codon, com

162 pyrrolysine with 20% efficiency, suggesting UAG translation in the absence of evolved context.

163 f the pseudohyphal phenotype, 10 novel sup70 UAG suppressor alleles were identified, defining positio

164 n vivo activity of the analogs in supporting UAG suppression in Escherichia coli bearing genes for Py

165 om Escherichia coli glutamine tRNA, suppress UAG, UAA and UGA termination codons, respectively, in a

166 he 22nd amino acid, is encoded by amber (TAG=UAG) codons in certain methanogenic archaea and bacteria

167 owing greater translational readthrough than UAG or UAA.

168 We hypothesized that UAG would oppose the effects of acylated ghrelin (AG) on

169 s, and release factor sequences suggest that UAG is not a typical stop signal in Pyl-utilizing archae

170 n receptor, preclinical studies suggest that UAG may promote beta-cell function.

171 This suggests that UAG is subject to target cell-mediated activation - a no

172 se factor 1, which works specifically at the UAG termination codon, we constructed Escherichia coli s

173 site cysteine codon has been replaced by the UAG amber codon.

174 lysine is a lysine derivative encoded by the UAG codon in methylamine methyltransferase genes of Meth

175 require that 5-10 % of ribosomes decode the UAG as an amino acid and continue translation to synthes

176 rmination product of mtmB1 and decreased the UAG-translation product, which nonetheless contained pyr

177 The electron density for the UAG-encoded residue is distinct from any of the 21 natur

178 We have identified the UAG-encoded residue in a 1.55 angstrom resolution struct

179 xon 17 was the largest exon and included the UAG translation termination site, AUUAAA polyadenylation

180 sequence (PYLIS) significantly increased the UAG-termination product of mtmB1 and decreased the UAG-t

181 Sequences upstream of the UAG codon allow formation of two competing structures, a

182 ysine into protein during translation of the UAG codon and suggests that MtbB and MttB may exploit th

183 eudoknot located eight nucleotides 3' of the UAG is required for this redefinition of the UAG stop co

184 UAG is required for this redefinition of the UAG stop codon.

185 y reassigned the translation function of the UAG stop codon; however, reassigning sense codons poses

186 adipocytes show prominent expression of the UAG-activating enzyme ghrelin O-acyl transferase (GOAT),

187 amino acid incorporation in response to the UAG codon without increasing readthrough of other stop c

188 sis system that site-specifically--using the UAG amber codon--inserts Sec depending on the elongation

189 A is optimally active, the CAT gene with the UAG initiation codon produced more CAT protein (3- to 9-

190 ression of the suppressed construct with the UAG stop codon; tryptophan addition also resulted in ca.

191 bicyclomycin, an inhibitor of Rho, to these UAG constructs increases expression, demonstrating that

192 Translation of this UAG requires the aminoacylation of the corresponding amb

193 (tRNA(Pyl)) that presumably recognizes this UAG codon.

194 utation in Saccharomyces cerevisiae tRNA(Thr)UAG confers tRNA recognition by AgAlaRS.

195 sts of nine triplet repeats (five GAG, three UAG, and one AAG) that surround and overlap the trpP Shi

196 rying an initiation codon change from AUG to UAG.

197 1 was 1% of cellular protein with only trace UAG-terminated mtmB1 product detectable.

198 nes did not produce pyrrolysine or translate UAG as pyrrolysine.

199 lysine is a likely first step in translating UAG amber codons as pyrrolysine in certain methanogens.

200 Aminoacylation of tRNA(CUA)Tyr [tyrT (UAG)] by GlnRS-D235H resulted in a 4-fold increase in th

201 sed on pBR322 which no longer mischarge tyrT(UAG) in vivo.

202 he decoding of one of three stop codons UAA, UAG or UGA by the eukaryotic release factor eRF1.

203 signaled by any one of the stop codons UAA, UAG, and UGA moving into the ribosomal A site.

204 hree universally conserved stop codons: UAA, UAG or UGA.

205 ature termination codons (PTCs), either UAA, UAG, or UGA.

206 use the standard genetic code recognize UAA, UAG, and UGA as stop codons, whereas variant code specie

207 nd in vivo of terminating translation at UAA/UAG codons.

208 In addition, unambiguous UAG stop signals could not be identified.

209 We show that a newly uncovered UAG-encoded amino acid, desmethylpyrrolysine, is made fr

210 5-fold by placing the murine leukemia virus UAG read-through element upstream of the first UGA codon

211 ression 60-fold when the tnaC stop codon was UAG and 3-fold when this stop codon was UAA; basal level

212 However, when UAG or UAA replaced UGA, the induced level of expression

213 with glutamine but extremely poorly, whereas UAG could not be used to initiate protein synthesis with

214 ndings are best explained by a model wherein UAG codons may have ambiguous meaning and Pyl insertion

215 native proteins in E. coli strains in which UAG was reassigned to encode phosphoserine.

216 of leadered, but not unleadered, mRNAs with UAG start codons, indicating that codon-anticodon comple

217 acing the natural tnaC stop codon, UGA, with UAG or UAA in a tnaC-stop codon-tnaA'-'lacZ reporter con