ライフサイエンスコーパス: UDP-Gal

1 UDP-Gal(f) was detected in wild-type animals while absen

2 UDP-Gal:betaGlcNAc beta1,3-galactosyltransferase (Gal-T-

3 ormation (apo-enzyme), its Mn(2+) and Mn(2+)-UDP-Gal-bound complexes, and of a pentenary complex of b

4 Klebsiella pneumoniae sonicate could add 3H-UDP-Gal to human RBCs in the alpha configuration at 37 d

5 d in Lec8 cells corrected by expression of a UDP-Gal transporter cDNA.

6 a long flexible loop, which upon Mn(2+) and UDP-Gal binding changes from an open to a closed conform

7 Treatment of TNFR-IgG with beta1,4GT and UDP-Gal, in the presence of MnCl(2), followed by MALDI-T

8 calculated kcat/Km values for UDP-GalNAc and UDP-Gal are approximately 2-4 times higher than those fo

9 ient for the epimerization of UDP-GalNAc and UDP-Gal.

10 homodimer that can interconvert UDP-Glc and UDP-Gal but not UDP-GlcNAc and UDP-GalNAc.

11 ulated abnormally high levels of Gal-1-P and UDP-Gal and abnormally low levels of UDP-Glc and UDP-Glc

12 the double displacement of both glu-1-P and UDP-Gal.

13 te the continued accumulation of Gal-1-P and UDP-Gal.

14 e developed a nonradioactive method to assay UDP-Gal:beta-d-GlcNAcbeta1,4-galactosyltransferase (beta

15 rotein was assayed for activity against both UDP-Gal and UDP-GalNAc.

16 on, (ii) growth on galactose, and (iii) both UDP-Gal and UDP-GalNAc 4-epimerase activities in cell ex

17 Thus, provision of both UDP-Gal and UDP-GalNAc for RPS production by S. gordonii

18 ty through its role in stabilizing the bound UDP-Gal in a suitable conformation for catalysis.

19 The galactose moiety of the bound UDP-Gal molecule forms seven hydrogen bonds with the pro

20 -T1), upon the binding of Mn(2+) followed by UDP-Gal, two flexible loops, a long and a short loop, ch

21 ing functional GalE1 but not GalE2 contained UDP-Gal 4-epimerase but not UDP-GalNAc 4-epimerase activ

22 pocket closure as seen for the corresponding UDP-Gal derivative.

23 90 is critical in the binding of sugar donor UDP-Gal, whereas 174DYD176 may participate in the bindin

24 A resolution in complex with natural donors UDP-Gal, UDP-Glc and, in an attempt to overcome one of t

25 tures of M344H-Gal-T1 in complex with either UDP-Gal.Mn(2+) or UDP-Gal.Mg(2+), determined at 2.3 A re

26 hed that two genes (LPG5A and LPG5B) encoded UDP-Gal NSTs.

27 Extracellular UDP-Gal was detected on resting cultures of various cell

28 is remarkably stable, has an apparent Km for UDP-Gal of 630 microm and an apparent Vmax of 206 microm

29 increase in steady-state levels of mRNA for UDP-Gal:beta-D-Gal alpha1,3-galactosyltransferase (alpha

30 uct, and here we focused on transporters for UDP-Gal.

31 nsfers in the presence of manganese Gal from UDP-Gal to an acceptor sugar (xylose) that is attached t

32 on, an activity that could transfer Gal from UDP-Gal to both a Fuc-terminated glycoform of Skp1 and s

33 1 is synthesized by the transfer of Gal from UDP-Gal to GalNAcalpha1-R by core 1 beta3-galactosyltran

34 ltransferase (T-synthase) transfers Gal from UDP-Gal to GalNAcalpha1-Ser/Thr (Tn antigen) to form the

35 I (beta4Gal-T1) normally transfers Gal from UDP-Gal to GlcNAc in the presence of Mn(2+) ion (Gal-T a

36 erase I (Gal-T1) normally transfers Gal from UDP-Gal to GlcNAc in the presence of Mn(2+) ion.

37 he presence of manganese, transfers Gal from UDP-Gal to GlcNAc.

38 transfer GalNAc from UDP-GalNAc or Gal from UDP-Gal to the H-antigen acceptor.

39 se 1 (Gal-T1) transfers galactose (Gal) from UDP-Gal to N-acetylglucosamine (GlcNAc), which constitut

40 l-T activity that transferred galactose from UDP-Gal to GalNAcalpha1-O-phenyl, and a synthetic glycop

41 ified GALS1 could transfer Gal residues from UDP-Gal onto beta-1,4-galactopentaose.

42 membranes transfer galactosyl residues from UDP-Gal to diacylglycerol.

43 alpha3GT catalyzes galactose transfer from UDP-Gal to beta-linked galactosides, such as lactose, an

44 ose (Gal), and uridyl diphosphate galactose (UDP-Gal) confirms type I, II, and III galactosemia disea

45 phosphate (gal-1-P) is bound, UDP-galactose (UDP-Gal) is released, and the free enzyme is recycled.

46 ion transfers galactose from UDP-galactose (UDP-Gal) to N-acetylglucosamine (GlcNAc) that is either

47 mono-functional, synthesising UDP-galactose (UDP-Gal), or bi-functional, synthesising UDP-Gal and UDP

48 ted by UDP-glucose (UDP-Glc), UDP-galactose (UDP-Gal), UDP-N-acetylglucosamine (UDP-GlcNAc), and UDP-

49 acturonic acid (UDP-GalA) and UDP-galactose (UDP-Gal).

50 opsis AtUGlcAE1 is not inhibited by UDP-Glc, UDP-Gal, or UMP.

51 sis, namely, those for formation of UDP-Glc, UDP-Gal, UDP-GalNAc, and dTDP-Rha.

52 yme activity, including UDP-GalNAc, UDP-Glc, UDP-Gal, UDP-GalUA, UMP, UDP, and UTP.

53 The Km values of Gne for UDP-Glc, UDP-Gal, UDP-GlcNAc, and UDP-GalNAc are 370, 295, 323, a

54 e cells showed concentrations of UDP-GlcNAc, UDP-Gal, UDP-Glc, GDP-Fuc, and GDP-Man equal to or highe

55 tant by heterologous expression of the human UDP-Gal transporter, and heterologous expression of LPG5

56 Both of these cell lines are defective in UDP-Gal 4-epimerase and cannot synthesize GM3 unless cul

57 inese hamster ovary ldl-D cells defective in UDP-Gal/UDP-GalNAc 4-epimerase in which N- and O-linked

58 nt cells, a mammalian cell line deficient in UDP-Gal transport into the Golgi.

59 mammalian Lec8 mutant, which is deficient in UDP-Gal uptake.

60 regeneration of sugar nucleotides, including UDP-Gal, UDP-GalNAc, GDP-Fuc, and CMP-Neu5Ac.

61 action in CHS, rs686237, strongly influenced UDP-Gal:betaGlcNAc beta-1,4-galactosyltransferase, polyp

62 n from the precursor GalNAc-alpha-Ser/Thr is UDP-Gal:GalNAc-alpha-Ser/Thr beta3-galactosyltransferase

63 ucleotides (CMP-Neu5Ac, CMP-Neu5Gc, CMP-KDN, UDP-Gal, UDP-Glc, UDP-GalNAc, UDP-GlcNAc, GDP-Fuc, GDP-M

64 structure of R228K-Gal-T1 complexed with LA, UDP-Gal, and Mn(2+) determined at 1.9 A resolution shows

65 Chinese hamster ovary (CHO) cells that lack UDP-Gal transporter activity and express GlcNAc-terminat

66 (ldlD) cells that lack the epimerase to make UDP-Gal/GalNAc from UDP-Glc/GlcNAc.

67 a-(32)P]UTP was linear between 0.5 and 30 nM UDP-Gal.

68 letion mutant showed loss of GDP-Man but not UDP-Gal uptake, which was restored by introduction of th

69 8 cells was active using UDP-GalNAc, but not UDP-Gal, as a donor toward a variety of acceptor substra

70 Simultaneous and independent binding of UDP-Gal and CMP-Sia was seen in the absence of an accept

71 Thus, the binding of UDP-Gal induces a conformational change in Gal-T1, which

72 nding to Gal-T1 is similar to the binding of UDP-Gal to Gal-T1, except for an additional hydrogen bon

73 The overall conversion of UDP-Gal to [gamma-(32)P]UTP was linear between 0.5 and 3

74 ssay is based on the enzymatic conversion of UDP-Gal to UDP, using 1-4-beta-galactosyltransferase.

75 quent binding of gal-1-P and displacement of UDP-Gal.

76 DP-Gal, probably causing the dissociation of UDP-Gal and the reduced k(cat) of the Gal-T reaction.

77 4-epimerase catalyzes the interconversion of UDP-Gal and UDP-Glc during normal galactose metabolism.

78 The galactose moiety of UDP-Gal is found deep inside the catalytic pocket, inter

79 act with the 2-OH of the galactose moiety of UDP-Gal, is a key residue in the stringent donor substra

80 o the O4 hydroxyl group of the Gal moiety of UDP-Gal, probably causing the dissociation of UDP-Gal an

81 describe an assay for the quantification of UDP-Gal with subnanomolar sensitivity.

82 The occurrence of regulated release of UDP-Gal suggests that, in addition to its role in glycos

83 various cell types, and increased release of UDP-Gal was observed in 1321N1 human astrocytoma cells s

84 Comparison of the crystal structures of UDP-Gal- and UDP-Glc-bound beta4Gal-T1 reveals that the

85 Moreover, AtGALT2 uses only UDP-Gal as the substrate donor and requires Mg(2+) or Mn

86 -T1 in complex with either UDP-Gal.Mn(2+) or UDP-Gal.Mg(2+), determined at 2.3 A resolution, show tha

87 ctures shows that, upon manganese and UDP or UDP-Gal binding, the enzyme undergoes conformational cha

88 can be glycosylated by UDP-Glc, UDP-Xyl, or UDP-Gal, and isolated a cDNA encoding it, apparently der

89 stal structure reported earlier, the present UDP-Gal bound structure exhibits a large conformational

90 tion to its role in glycosylation reactions, UDP-Gal is an important extracellular signaling molecule

91 substrate xylobiose and the donor substrate UDP-Gal, respectively.

92 se (UDP-Gal), or bi-functional, synthesising UDP-Gal and UDP-galactosamine (UDP-GalNAc).

93 er" conformation previously observed for the UDP-Gal donor.

94 found that levels of Gg4 and of mRNA for the UDP-Gal:beta1-3galactosyltransferase-4 (beta3GalT4) gene

95 ns were able to convert UDP-glucose (Glc) to UDP-Gal, but only the BAS5304-encoded protein could conv

96 a loss of epimerase activity with regard to UDP-Gal by almost 5-fold, it resulted in a gain of activ

97 ence for UDP-GalNAc as substrate relative to UDP-Gal.

98 h the binding of UDP-Glc is quite similar to UDP-Gal, there are few significant differences observed

99 SQV-7 is able to transport UDP-Gal in vivo, as shown by its ability to complement t

100 TbNST1/2 transports UDP-Gal/UDP-GlcNAc, TbNST3 transports GDP-Man, and TbNST

101 f compounds was screened for M. tuberculosis UDP-Gal mutase inhibition.

102 Addition of unlabeled UDP-Gal enhances Sia-FITC incorporation, indicating a su

103 Although these galactosyltransferases use UDP-Gal as the galactose donor, a third pathway involves

104 The MIC activity did not correlate with UDP-Gal mutase inhibition, suggesting an alternative pri

105 syltransferase (Gal-T1) co-crystallized with UDP-Gal and MnCl(2) has been solved at 2.8 A resolution.

106 hough either Mn(2+) or Mg(2+), together with UDP-Gal, binds and changes the conformation of the M344H