ライフサイエンスコーパス: UDP-N-acetylglucosamine

1 le for instantaneous reaction with substrate UDP-N-acetylglucosamine.

2 the biosynthesis of this sugar starting from UDP-N-acetylglucosamine.

3 se, as well as UDP-N-acetylgalactosamine and UDP-N-acetylglucosamine.

4 interconverts UDP-N-acetylgalactosamine and UDP-N-acetylglucosamine.

5 he release of the second product enolpyruvyl-UDP-N-acetylglucosamine.

6 of substrates: UDP-N-acetylgalactosamine and UDP-N-acetylglucosamine.

7 aving endogenous transport activity for only UDP- N-acetylglucosamine.

8 RNA), lipoprotein YceK, toxin HicA, or MurA (UDP-N-acetylglucosamine 1-carboxyvinyltransferase) suppr

9 Construction of a defined mutation in the UDP-N-acetylglucosamine-1-phosphate transferase gene, we

10 Construction of a defined mutation in the UDP-N-acetylglucosamine-1-phosphate transferase gene, we

11 se-1-phosphate uridylyltransferase (galU), a UDP-N-acetylglucosamine 2-epimerase (wecB) and a UDP-N-a

12 gC, are shown by complementation to encode a UDP-N-acetylglucosamine 2-epimerase and a UDP-N-acetylma

13 ingle protein with key enzymatic activities, UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosam

14 imply that the neuC gene product encodes an UDP-N-acetylglucosamine 2-epimerase that generates ManNA

15 Using UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine

16 synthesis of sialic acid is the bifunctional UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine

17 n N-acetylmannosamine kinase (MNK) domain of UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine

18 have cloned and expressed the gene encoding UDP-N-acetylglucosamine 3-O-acyltransferase of C. tracho

19 UDP-N-acetylglucosamine-3-O-acyltransferase (UDP-GlcNAc

20 ractions in the presence of 10 mM DTT, shows UDP-N-acetylglucosamine 6-dehydrogenase activity and is

21 n of glucosamine-1-P, UTP, and acetyl-CoA to UDP-N-acetylglucosamine, a fundamental precursor in bact

22 UDP-N-acetylglucosamine acyltransferase (LpxA) and UDP-3

23 UDP-N-acetylglucosamine acyltransferase (LpxA) and UDP-3

24 UDP-N-acetylglucosamine acyltransferase (LpxA) initiates

25 These acyl chains are attached by UDP-N-acetylglucosamine acyltransferase (LpxA).

26 An lpxA gene encoding UDP-N-acetylglucosamine acyltransferase responsible for

27 CtLpxA is the first reported UDP-N-acetylglucosamine acyltransferase that prefers a n

28 omplex of the acyltransferase protein, LpxA (UDP-N-acetylglucosamine acyltransferase), and acyl carri

29 lating the maturation of N-linked glycans is UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-a

30 Here, the involvement of UDP-N-acetylglucosamine:alpha-6-D-mannoside beta1,6-N-ac

31 e identified as two uridylated amino sugars, UDP N-acetylglucosamine and UDP N-acetylgalactosamine.

32 hlights the residues important in binding of UDP-N-acetylglucosamine and 1-L-myo-inositol-1-phosphate

33 tivated as indicated by over 2-fold elevated UDP-N-acetylglucosamine and glycogen.

34 product state of the enzyme with enolpyruvyl-UDP-N-acetylglucosamine and inorganic phosphate trapped

35 e show that the N-acetylglucosamine donor is UDP-N-acetylglucosamine and that the N-acetylglucosamine

36 s, K(M), of the P. multocida HA synthase for UDP-N-acetylglucosamine and UDP-glucuronic acid were est

37 -3), all producing an identical polymer from UDP-N-acetylglucosamine and UDP-glucuronic acid.

38 We have characterized a transporter of UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine en

39 L2 in the apo-form and with donor substrates UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine.

40 transporter for UDP-glucose, UDP-galactose, UDP- N-acetylglucosamine, and UDP- N-acetylgalactosamine

41 aining cell wall precursors, UDP-Glucose and UDP-N-acetylglucosamine are efficiently used to initiate

42 hich use cytoplasmic UDP-glucuronic acid and UDP-N-acetylglucosamine as substrates.

43 on of successive coupled enzyme assays using UDP-n-acetylglucosamine as the initial sugar substrate.

44 shown in digitonin-permeabilized cells, with UDP-N-acetylglucosamine as the substrate for nascent chi

45 tion mutant of the C03H5.2 protein transport UDP-N-acetylglucosamine at rates comparable with that of

46 each mutation using both the UDP-glucose and UDP-N-acetylglucosamine bound structures of the wild-typ

47 arbanion during the reduction of enolpyruvyl-UDP-N-acetylglucosamine catalyzed by the bacterial pepti

48 Glucosamine infusion increased muscle UDP-N-acetylglucosamine concentrations 3.9- and 4.3-fold

49 of enolpyruvate from phosphoenolpyruvate to UDP-N-acetylglucosamine, confirming they are both active

50 strate-free MurB and MurB complexed with the UDP-N-acetylglucosamine enolpyruvate (UNAGEP) substrate.

51 essential peptidoglycan biosynthetic enzyme, UDP-N-acetylglucosamine enolpyruvoyl transferase (MurA).

52 GlcNAc) and phosphoenolpyruvate catalyzed by UDP-N-acetylglucosamine enolpyruvoyl transferase.

53 UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) a

54 Gene sequences encoding the enzymes UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) f

55 s harbour two genes (murA and murZ) encoding UDP-N-acetylglucosamine enolpyruvyl transferase activity

56 chlamydial anomaly by characterizing MurA, a UDP-N-acetylglucosamine enolpyruvyl transferase that cat

57 uncC (proton-translocating ATPase) and murA (UDP-N-acetylglucosamine enolpyruvyl transferase).

58 MurA (UDP-N-acetylglucosamine enolpyruvyl transferase, EC 2.5.

59 MurA (UDP-N-acetylglucosamine enolpyruvyl transferase, EC 2.5.

60 glucosamine, confirming they are both active UDP-N-acetylglucosamine enolpyruvyl transferases.

61 ne biosynthetic pathway (HBSP) that produces UDP-N-acetylglucosamine for O-linked N-acetylglucosamine

62 a pre-steady-state lag in the production of UDP-N-acetylglucosamine from acetyl-CoA, UTP, and glucos

63 was competent in catalyzing the formation of UDP-N-acetylglucosamine from UTP and N-acetylglucosamine

64 ied the pools of UDP-glucose, UDP-galactose, UDP-N-acetylglucosamine, GDP-mannose, and GDP-fucose in

65 ence of NeuC showed similarities to those of UDP-N-acetylglucosamine (GlcNAc) 2-epimerases from both

66 A radioenzymatic synthesis of [32P]5-azido-UDP-N-acetylglucosamine has been accomplished using 5-az

67 daemia, which also increase tissue levels of UDP-N-acetylglucosamine in conscious rodents.

68 s specifically defective in the transport of UDP- N-acetylglucosamine into its Golgi apparatus.

69 ied S. aureus MGT catalyzed incorporation of UDP-N-acetylglucosamine into peptidoglycan, proving that

70 ich the activity of the uridine diphosphate (UDP)-N-acetylglucosamine:lysosomal enzyme N-acetylglucos

71 alpha- and beta-subunits of purified bovine UDP-N-acetylglucosamine:lysosomal enzyme N-acetylglucosa

72 This modification is catalyzed by UDP-N-acetylglucosamine:lysosomal enzyme N-acetylglucosa

73 The kinetic properties of UDP-N-acetylglucosamine:lysosomal-enzyme N-acetylglucosa

74 UDP-N-acetylglucosamine:lysosomal-enzyme N-acetylglucosa

75 p in the synthesis of mannose 6-phosphate is UDP-N-acetylglucosamine:lysosomal-enzyme-N-acetylglucosm

76 md8Delta was reduced by deletion of the YEA4 UDP- N-acetylglucosamine or the HUT1 UDP-galactose trans

77 ring the reaction of free MurA and substrate UDP-N-acetylglucosamine or isomer UDP-N-acetylgalactosam

78 ationally predicted putative miR-185 targets UDP-N-acetylglucosamine-peptide N-acetylglucosaminyltran

79 Two intracellular enzymes, UDP-N-acetylglucosamine-polypeptide beta-N-acetylglucosa

80 ion requires the presence of substrate UNAG (UDP-N-acetylglucosamine), proceeding with an inactivatio

81 n which the beta-phosphate of the substrate, UDP-N-acetylglucosamine, promotes the nucleophilic attac

82 UDP-N-acetylglucosamine pyrophosphorylase (UAP) is the f

83 ncluding Hill plots demonstrate clearly that UDP-N-acetylglucosamine pyrophosphorylase activity, puri

84 UDP-N-acetylglucosamine pyrophosphorylase immunoaffinity

85 mmy codes for the single UDP-N-acetylglucosamine pyrophosphorylase in Drosophila,

86 Thus, the data support the idea that UDP-N-acetylglucosamine pyrophosphorylase is a major reg

87 UDP-N-acetylglucosamine pyrophosphorylase protein is pre

88 ylgalactosamine kinase, and Escherichia coli UDP-N-acetylglucosamine pyrophosphorylase, GlmU.

89 doglycan and that includes the UTP-requiring UDP-N-acetylglucosamine pyrophosphorylase.

90 A with the substrate analog, (E)-enolbutyryl-UDP-N-acetylglucosamine, showed a striking bias of the p

91 zes the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to 1-L-myo-inositol-1-phosphate

92 e/N-acetylmannosamine kinase that transforms UDP-N-acetylglucosamine to N-acetylmannosamine (ManNAc)

93 is catalyzed by an unusual hetero-oligomeric UDP-N-acetylglucosamine transferase that in most eukaryo

94 erases I and II, and uridine 5'-diphosphate (UDP)-N-acetylglucosamine transporter.

95 SLC35A3 is considered the main UDP-N-acetylglucosamine transporter (NGT) in mammals.

96 UDP-galactose transporter (UGT; SLC35A2) and UDP-N-acetylglucosamine transporter (NGT; SLC35A3) form

97 UDP-N-acetylglucosamine (UDP-GlcNAc) acyltransferase (Lp

98 UDP-N-acetylglucosamine (UDP-GlcNAc) acyltransferase (Lp

99 At5g65000) as an ER-localized facilitator of UDP-N-acetylglucosamine (UDP-GlcNAc) and UDP-N-acetylgal

100 Here we show that TpeL preferably utilizes UDP-N-acetylglucosamine (UDP-GlcNAc) as a sugar donor.

101 he hexosamine biosynthetic pathway, increase UDP-N-acetylglucosamine (UDP-GlcNAc) availability, and l

102 ge, and residues predicted to be involved in UDP-N-acetylglucosamine (UDP-GlcNAc) donor specificity.

103 The sugar nucleotide UDP-N-acetylglucosamine (UDP-GlcNAc) is an essential met

104 The HBP end product UDP-N-acetylglucosamine (UDP-GlcNAc) is used in enzymati

105 is in Gram-negative bacteria is catalyzed by UDP-N-acetylglucosamine (UDP-GlcNAc) O-acyltransferase,

106 r polysaccharide (CP5) is synthesized from a UDP-N-acetylglucosamine (UDP-GlcNAc) precursor that is e

107 Strikingly, addition of the HBP metabolite UDP-N-acetylglucosamine (UDP-GlcNAc) to CRPC-like cells

108 ses the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine (UDP-GlcNAc) to serines and thre

109 etyl-glucosamine 6-dehydrogenase, converting UDP-N-acetylglucosamine (UDP-GlcNAc) to UDP-N-acetyl-glu

110 d is capable of catalyzing the conversion of UDP-N-acetylglucosamine (UDP-GlcNAc) to UDP-N-acetylgluc

111 The specificity of the UDP-N-acetylglucosamine (UDP-GlcNAc) translocator for th

112 yzes this post-translational modification is UDP-N-acetylglucosamine (UDP-GlcNAc), a product of the h

113 -glucose (UDP-Glc), UDP-galactose (UDP-Gal), UDP-N-acetylglucosamine (UDP-GlcNAc), and UDP-glucuronic

114 duct of the hexosamine biosynthetic pathway, UDP-N-acetylglucosamine (UDP-GlcNAc), result in rapid an

115 ecursors, UDP-glucuronic acid (UDP-GlcA) and UDP-N-acetylglucosamine (UDP-GlcNAc), were utilized to p

116 In complex with the cosubstrate UDP-N-acetylglucosamine (UDP-GlcNAc),O-linked-GlcNAc tra

117 olecules UDP-glucuronic acid (UDP-GlcUA) and UDP-N-acetylglucosamine (UDP-GlcNAc).

118 The enzyme UDP-N-acetylglucosamine (UDP-NAG) enolpyruvyltransferase

119 se UDP-glucuronic acid, and UGT3 enzymes use UDP-N-acetylglucosamine, UDP-glucose, and UDP-xylose to

120 d not transport CMP-sialic acid, GDP-fucose, UDP-N-acetylglucosamine, UDP-glucose, or GDP-mannose.

121 of MurA with respect to the first substrate, UDP-N-acetylglucosamine (UNAG), with a K(i) of 16 microM

122 higher than that of GFAT1, whereas K(i) for UDP-N-acetylglucosamine was approximately fivefold lower

123 [32P]5-Azido-UDP-N-acetylglucosamine was functionally characterized u

124 in the presence of the substrate enolpyruvyl-UDP-N-acetylglucosamine were solved and refined at 1.8 A

125 rconversion of UDP-N-acetylgalactosamine and UDP-N-acetylglucosamine while the bacterial enzyme canno