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1 UMP also significantly increased plasma and brain cytidi
2 UMP and CMP proved to be far better substrates than dCMP
3 UMP and GMP, accompanied by Mg2+, bind specifically to P
4 UMP and phosphoribosylpyrophosphate were shown by equili
5 UMP synthase, the bifunctional protein that catalyzes th
6 UMP was released from the enzyme concomitantly with CO(2
7 UMP, GMP, and CMP could not substitute for AMP as gamma-
8 UMP, which acted as a competitive inhibitor of UDP-Glc,
9 UMP/CMPK localized predominantly to the cytoplasm.
10 UMP/CMPK used all of the nucleoside triphosphates as pho
11 erase (E-His(166) + UDP-glucose = E-His(166)-UMP + glucose-1-P) is found to be 1.8 x 10(-4) at pH 7.0
12 ) for the hydrolyses of UMPIm and E-His(166)-UMP are -14.7 and -15.4 kcal mol(-1), respectively at pH
13 active site and the UMP group of E-His(166)-UMP provide little or no stabilization in the formation
15 66A RNase A and the K7A/R10A/K66A RNase A.3'-UMP complex were determined by X-ray diffraction analysi
19 s consistent with the phosphoryl group of 3'-UMP interacting more strongly with the two active-site h
20 e presence of different concentrations of 3'-UMP shows that a second molecule of 3'-UMP can bind to t
21 e reaction product, uridine 3'-phosphate (3'-UMP), protonation of one active-site histidine residue f
22 f wild-type RNase A and the variants with 3'-UMP at pH 6.0 show that His12 and His119 contribute 1.4
23 te (5'-AMP) and uridine 5'-monophosphate (5'-UMP) are synthesized, and their structures are elucidate
27 d CDP-choline were all elevated 15 min after UMP (from 254 +/- 31.9 to 417 +/- 50.2, [P < 0.05]; 56.8
37 o form 2,3-diacylglucosamine 1-phosphate and UMP by catalyzing the attack of water at the alpha-P ato
42 Binding of PyrR to pyr RNA was specific and UMP-dependent with apparent Kd values of 10 and 220 nM i
43 ternary complex composed of UTP, TbTUT4, and UMP, which mimics an RNA substrate, and the postreaction
44 llular UTP or its breakdown products UDP and UMP act as mediators for hyaluronan synthase (HAS) activ
45 syltransferase activity of PyrR, antagonized UMP-dependent transcriptional termination, but uracil di
46 e slow deuridylylation of GlnK approximately UMP by the UTase/UR suggests that rapid interconversion
47 wed the deuridylylation of PII approximately UMP upon ammonia addition, showing that multiple PII int
48 ng enzyme (UTase/UR), only PII approximately UMP was effectively deuridylylated by the UR activity of
51 nstant = 23.2 microm), and the T-state binds UMP exclusively (dissociation constant = 0.49 microm).
55 ers for deuterium exchange into enzyme-bound UMP and F-UMP of 1.2 x 10(-5) and 0.041 s(-1), respectiv
57 active triphosphate metabolite, PSI-7409, by UMP-CMP kinase and nucleoside diphosphate kinase, respec
58 terminator structures, and that PyrR acts by UMP-dependent binding to and stabilization of the anti-a
60 steps in de novo synthesis are catalysed by UMP synthase (UMPS) - a bifunctional enzyme comprising o
61 w kinetic studies on UTP binding followed by UMP incorporation into an EC as monitored by alterations
62 yrimidines, and its activity is inhibited by UMP and activated by 5-phospho-alpha-D-ribosyl diphospha
64 ressed as a bifunctional gene product called UMP synthase (UMPS), with OPRT at the N terminus and OMP
65 ) is responsible for phosphorylation of CMP, UMP, and deoxycytidine monophosphate (dCMP) and also pla
69 phosphate) versus their ribose-counterparts (UMP and 5-fluorouridine monophosphate), in a similar man
70 cement, with the participation of a covalent UMP-His 166-enzyme intermediate in the Escherichia coli
71 en atoms of the substrate in the crystalline UMP-enzyme and in the crystalline complex of H166G-GalT
74 tate based on the structure of D. discoideum UMP/CMP kinase aids to rationalize the substrate specifi
75 ted for the similar Dictyostelium discoideum UMP/CMP kinase reveals the conformational changes that o
79 enzyme activity was correlated with elevated UMP synthase protein levels as well as elevated UMP synt
84 uterium exchange into enzyme-bound UMP and F-UMP of 1.2 x 10(-5) and 0.041 s(-1), respectively, so th
87 or k(cat)/K(m) for deuterium exchange into F-UMP gives the intrinsic second-order rate constant for e
88 UMP) and 5-fluorouridine 5'-monophosphate (F-UMP) catalyzed by yeast orotidine 5'-monophosphate decar
90 levels; however, both basally and following UMP, these levels were much lower than those of uridine.
92 rification scheme, in which the affinity for UMP was markedly reduced, CP rate curves showed no sigmo
94 ion suggests that exogenous Up4A first forms UMP and ATP in the human colon and UDP and ADP in the mu
99 inhibited by NH(4)(+), suggesting that GlnK-UMP is required to signal adenylyltransferase/adenylyl-r
101 AMP, ADP, ATP, CMP, CDP, CTP, GMP, GDP, GTP, UMP, UDP, and UTP) was examined by reversed-phase HPLC a
105 data suggest that the active sites of human UMP/CMP kinase for dCMP and for CMP cannot be identical.
108 a using recombinant or highly purified human UMP/CMP kinase showed that dCMP, as well as pyrimidine a
109 ction conditions for human recombinant human UMP/CMP kinase to phosphorylate dCMP and CMP (referred a
112 s, we hypothesize that the covalent union in UMP synthase stabilizes the domains containing the respe
115 osteric activator ornithine or the inhibitor UMP despite the substantial and opposing effects these l
117 er, as a non-heme, Fe(II)-dependent alpha-KG:UMP dioxygenase that produces uridine-5'-aldehyde to ini
118 eoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK);EC 2.7.4.14] plays a crucial role in the forma
119 Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK);EC 2.7.4.14] plays a crucial r
121 eaction in which the enzyme covalently links UMP to the hydroxyl group of tyrosine in the terminal pr
122 ermined to be K(m)(alpha-KG) = 7.5 muM, K(m)(UMP) = 14 muM, and k(cat) approximately 80 min(-1).
125 the C-6 protons of uridine 5'-monophosphate (UMP) and 5-fluorouridine 5'-monophosphate (F-UMP) cataly
126 o steps of de novo uridine 5'-monophosphate (UMP) biosynthesis are catalyzed by orotate phosphoribosy
127 the uridine source uridine-5'-monophosphate (UMP) can significantly increase levels of the phosphatid
128 hways whereby oral uridine-5'-monophosphate (UMP) increases membrane phosphatide synthesis in brains
129 phosphate (CP) and uridine 5'-monophosphate (UMP), showed pronounced sigmoidicity, each in the presen
130 a uridine source, uridine-5'-monophosphate (UMP), which increases brain levels of the rate-limiting
132 abidopsis thaliana uridine 5'-monophosphate (UMP)/cytidine 5'-monophosphate (CMP) kinase was isolated
134 hosphate (AMP) and 5'-uridine monophosphate (UMP) molecules confined in multi-lamellar phospholipid b
135 the conversion of 5'-uridine monophosphate (UMP) to UMP-NeuAc, which was found to be an inactive sia
136 nophosphate (IMP) and uridine monophosphate (UMP) were only 30% of those found in the wild-type cells
137 y annotating N-methyl-uridine monophosphate (UMP), lysomonogalactosyl-monopalmitin, N-methylalanine,
139 supplemental uridine (as its monophosphate, UMP; 0.5%) and choline (0.1%) via the diet, and docosahe
141 s have activities very similar to the native UMP synthase, but unlike the bifunctional protein, the d
146 espect to uptake of orotic acid, affinity of UMP synthase for its substrates, or UMP synthase gene-co
149 s is initiated by the covalent attachment of UMP to the terminal protein VPg, yielding VPgpU and VPgp
156 n vitro, at physiological concentrations, of UMP synthase, the two isolated catalytic domains prepare
158 diastereomer mixture prepared by coupling of UMP-morpholidate with fluoromethylenebis(phosphonic acid
159 liganded conformation before dissociation of UMP and were inconsistent with several other kinetic mec
160 6-amino acid UMP/CMPK was the actual form of UMP/CMPK, rather than the 228-amino acid form as suggest
163 this investigation were the imidazolides of UMP, xanthosine 5'-monophosphate, the bis-monophosphates
166 e HIV protease and the unfolding kinetics of UMP/CMP kinase, a globular protein from Dictyostelium di
169 s 2 (HRV2) catalyzes the covalent linkage of UMP to the terminal protein (VPg) using poly(A) as a tem
173 replication is initiated when a molecule of UMP is covalently linked to the hydroxyl group of a tyro
174 replication is initiated when a molecule of UMP is covalently linked to the hydroxyl group of a tyro
180 ckettsial CTP appears to be the transport of UMP followed by its phosphorylation and the amination of
183 and in each major phosphatide; giving DHA or UMP (plus choline) produced smaller increases in some of
188 All three allosteric ligands, ornithine, UMP, and IMP, act by modifying the affinity of CPS for t
192 case, one would expect uridine 5'-phosphate (UMP) derivatives with bulky anionic substituents at C6 t
197 and PII-UMP, these domains bound PII and PII-UMP significantly better when linked to the central regi
199 ent with the protein activators (PII and PII-UMP) binding to the enzyme domain with the opposing acti
200 clusively through its binding to PII and PII-UMP, did not alter the binding of PII or PII-UMP to the
201 polypeptides were indifferent to PII and PII-UMP, or their ATase activity was inhibited by either PII
202 domains of ATase bound poorly to PII and PII-UMP, these domains bound PII and PII-UMP significantly b
209 g (AR) activity of ATase is activated by PII-UMP and inhibited by unmodified PII and by glutamine.
211 ed by glutamine and its level reduced by PII-UMP, whereas glutamine and PII-UMP competed for the enzy
217 Our results were consistent with PII, PII-UMP, and glutamine shifting the enzyme among at least si
220 The deduced amino acid sequence of the plant UMP/CMP kinase has 50% identity with other eukaryotic UM
221 Following proteolytic digestion, the plant UMP/CMP kinase was purified and analyzed for its structu
223 Transcriptional attenuation by PyrR plus UMP was also demonstrated in vitro with templates from t
225 ate, uracil (2.2 A resolution), its product, UMP (2.5 A resolution), and the prodrug, 5-fluorouracil
227 were measured using mixtures of radiolabeled UMP-NeuAc's as the donor substrate and N-acetyllactosami
228 In the present study, adult gerbils received UMP (1 mmol/kg), a constituent of human breast milk and
230 In particular, positions of the respective UMP alpha-phosphoryl groups differ by approximately 4 A.
231 5-fold tighter in the presence of saturating UMP or UDP and 150- fold tighter with saturating UTP, su
232 for the linkage of both the first and second UMPs to VPg, 3) VPgpUpU is synthesized by a "slide-back"
237 PyrR, but concentrations 10-fold higher than UMP were required; UDP was only effective at 100 times t
240 interactions between the active site and the UMP group of E-His(166)-UMP provide little or no stabili
241 kage is a phosphoramidate formed between the UMP moiety and the His 166 N(epsilon)(2) of GalT, with H
242 ption whereby termination is governed by the UMP-dependent binding of PyrR to pyr RNA and provide pur
244 onclusion derives from studies employing the UMP synthesis inhibitor N-phosphonacetyl-L-aspartate (PA
246 ferase (OMP synthase, EC 2.4.2.10) forms the UMP precursor orotidine 5'-monophophate (OMP) from orota
249 n bonding with the phosphoryl oxygens of the UMP moiety, which is bonded to His 166 in the intermedia
251 (ScOMPDC) catalyze the exchange of H6 of the UMP product with solvent deuterium allows an estimate of
252 tidylate decarboxylase (ODC) activity of the UMP synthase enzyme complex that catalyzes an early even
253 n authentic and well conserved member of the UMP/CMP kinase group while EhAK is the most divergent me
254 hat glutamine at position 188 stabilizes the UMP-GALT intermediate through hydrogen bonding and enabl
256 hat delivers a solvent-derived proton to the UMP product; the active site of KGPDC contains a homolog
257 active sites underwent uridylylation to the UMP-enzyme, similar to wild-type GalT, upon reaction wit
258 the presence of SII, incorporation of 4-thio-UMP into RNA, and irradiation and was sensitive to treat
261 he enzyme-catalyzed transformation of OMP to UMP may have less of an energetic impact than commonly t
265 lucosyltransferase inhibitory product UDP to UMP, and that the latter product then exits the lumen of
267 version of 5'-uridine monophosphate (UMP) to UMP-NeuAc, which was found to be an inactive sialyl dono
270 examined, but no catalysis of [(14)C]uracil-UMP and [(32)P]PP(i)-phosphoribosylpyrophosphate exchang
273 TP (Michaelis constant [Km] = 29 microM when UMP is the other substrate and Km = 292 microM when CMP
275 oline (ACh) synthesis, we determined whether UMP administration also affects ACh levels in striatum a
276 CP rate curves showed no sigmoidicity, while UMP rate curves had sigmoidicity exaggerated by a low ma
280 0-A resolution structure of the complex with UMP/GCP and a 2.8-A resolution structure of the complex
281 nor are they removed from an FAD resin with UMP (which shares a pattern of hydrogen bond donors and
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