ライフサイエンスコーパス: UTP

1 UTP (a P2Y(2) and P2Y(4) agonist) increased excitability

2 UTP activation of purinergic receptors and hydrolysis of

3 UTP also facilitated bladder neuron homomeric P2X(2) sus

4 UTP also increased expression of vitronectin, an extrace

5 UTP had only a slight effect, and GTP had almost none.

6 UTP increased calcium in pericytes leading to capillary

7 UTP increased hyaluronan both in the pericellular matrix

8 UTP increased the phosphorylation of p38, ERK, CREB, and

9 UTP or ADP stimulated afferents, including mouse and hum

10 UTP stimulation of endogenous P2Y receptors in COS 7 cel

11 UTP, in contrast, increases as the cells transition into

12 UTP-enriched TCs were found to be in two distinct fracti

13 UTP-gamma-aryl and cycloalkyl phosphoesters displayed on

14 UTP-glucose was inactive, but its (S)-methanocarba analo

15 UTP-induced phosphorylation of ErbB3 and EGFR was also i

16 UTP-induced spreading and migration of aortic SMCs did n

17 e pair (bp), fluorescein, and fluorescein-12-UTP (UTP) were separated in less than 120 s in channels

18 and dCTP bind in a very similar fashion, (2) UTP, in the presence of dCTP or CTP, binds at a site tha

19 CTP synthetase (EC 6.3.4.2, UTP:ammonia ligase (ADP-forming)) is an essential enzyme

20 P-labeled uridine-5'-triphosphate (alpha-32P-UTP) incorporation, nuclear accumulations of 99mTc-label

21 A levels was detected by both RT-PCR and 32P-UTP in these cells.

22 the fluorinated 5'-triphosphate analogues 5F-UTP and 5F-CTP have been developed to facilitate 19F-lab

23 he availability of efficient synthesis of 5F-UTP, and for the first time, 5F-CTP, will facilitate the

24 A UTP cocrystal structure of one mutant shows, in contrast

25 5'-deoxyuridine phosphorylase; (iii) LipM, a UTP:5-amino-5-deoxy-alpha-D-ribose-1-phosphate uridylylt

26 The presence of a UTP-stimulated, paxilline-sensitive large-conductance Ca

27 e family of glycosyl hydrolases, and UGP1, a UTP-glucose-1-phosphate uridylyltransferase which synthe

28 small interfering RNAs completely abolished UTP-activated sAPP alpha release.

29 ATP, ADP, UTP and beta-NAD elicited robust Ca(2+) transients in PD

30 purine receptor ligands (including ATP, ADP, UTP, UDP, and adenosine).

31 of 5'-nucleotidase had no effect on ATP/ADP/UTP-induced phospho- rylation of AMPK, indicating that A

32 P2Y2 (UTP/ATP) and P2Y6 [ADP/UTP/uridine 5'-diphosphate (UDP)] have been shown to hav

33 ensitive current increased immediately after UTP stimulation and was K(+) dependent.

34 ation of FLNa induced by the P2Y(2)R agonist UTP.

35 P2Y(2) nucleotide receptor (P2Y(2)R) agonist UTP stimulates a strong and sustained release of LTA fro

36 ng the P2Y(2/4) receptor (P2Y(2/4)R) agonist UTP, electrical stimulation, or transgenic mice expressi

37 on of bradykinin or the P2Y-receptor agonist UTP augmented the nicotine-induced increase in CR to abo

38 In contrast, the P2Y(2) receptor agonist UTP caused negligible ATP release, despite promoting a r

39 cation of the selective P2Y receptor agonist UTP decreased peak amplitudes of alpha,beta-meATP-evoked

40 that the G-protein-coupled receptor agonist UTP induces the translocation of PKC delta into the nucl

41 The effects of the P2Y receptor agonist UTP on neuronal excitability and discharge properties we

42 Although UTP is the preferred phosphoryl donor for this reaction,

43 nd can be further inhibited by UTP, although UTP alone has little or no influence on activity; howeve

44 hat extracellular nucleotides (ATP, ADP, and UTP, but not UDP) and adenosine independently induce pho

45 re, ex vivo studies showed that both ATP and UTP (10 micromol/L) promoted migration of SMCs out of ao

46 Enzymatic removal of ATP and UTP (by apyrase or the expression of ectopic CD39) abrog

47 ate the caspase-dependent release of ATP and UTP (in equimolar quantities) during the early stages of

48 mRNA expression were upregulated by ATP and UTP but not ADP or adenosine in vitro.

49 , release of TSP-1 was stimulated by ATP and UTP but not by 2-methylthio-ADP or adenosine.

50 Apical release of ATP and UTP can activate P2Y(2) receptors in the aldosterone-sen

51 In the present study, we show that ATP and UTP have strikingly opposite effects on human acute myel

52 Extracellular ATP and UTP induce chemotaxis, or directed cell migration, by st

53 r (P2Y2R) activated by extracellular ATP and UTP molecules released following injury/stress.

54 Extracellular ATP and UTP nucleotides increase the proliferation and engraftme

55 is model, an initial stimulus evokes ATP and UTP release from a single cell.

56 The nucleotides ATP and UTP represent one class of find-me signals, but their me

57 ive EcCTPS crystals soaked in either ATP and UTP substrates or ADP and CTP products.

58 ne and pyrimidine nucleotides (e.g., ATP and UTP) are strongly implicated in this process following t

59 , extracellular nucleotides (such as ATP and UTP) have emerged as key immunomodulators.

60 lular purines and pyrimidines (e.g., ATP and UTP), released during bladder distension or from damaged

61 ltiple factors, such as nucleotides, ATP and UTP.

62 ed protein kinases or Akt inhibited ATP- and UTP-induced TSP-1 expression.

63 C5a-mediated actin polymerization, C5a- and UTP-stimulated intracellular calcium mobilization, and t

64 er, they all possess conserved catalytic and UTP recognition domains, often accompanied by various au

65 ditionally, the enzyme utilizes both CTP and UTP equally well as substrates.

66 arbamoylase is feedback inhibited by CTP and UTP in the presence of CTP.

67 ffects of allosteric effectors, ATP, CTP and UTP on the kinetics of the quaternary structure change o

68 rted by Tlc4, it was an inhibitor of CTP and UTP uptake and demonstrated a K(i) similar to that of GD

69 xpression stimulated by prostaglandin E2 and UTP was not observed in cells lacking Gbeta-subunits.

70 oncentration ratios of ATP/ADP, GTP/GDP, and UTP/UDP were 1.9 +/- 1.39, 1.12 +/- 0.50, and 0.79 +/- 0

71 CTP, GTP, and UTP, as well as ATP, supported transfer but with lesser

72 Many of the pools, including ATP, GTP, and UTP, begin to decrease while the cells are still in mid-

73 for ATP and undetectable with GTP, ITP, and UTP.

74 The crystal structures of apo MEAT1 and UTP-bound MEAT1 refined to 1.56 A and 1.95 A, respective

75 . aureus alpha-phosphoglucomutase (PgcA) and UTP:alpha-glucose 1-phosphate uridyltransferase (GtaB) h

76 an be limiting in phosphatide synthesis, and UTP, which activates P2Y receptors coupled to neurite ou

77 espite the fact that its precursors, UDP and UTP, are not.

78 show here that adenosine, ADP, ATP, UDP, and UTP all stimulate calcium mobilization in bone marrow-de

79 ADP, ATP, CDP, CTP, FAD, GDP, GTP, UDP, and UTP.

80 Apical UTP or ATP-gamma-S stimulation of monolayers mounted in

81 m serum starvation-induced apoptosis by ATP, UTP, and ATPgammaS, an effect mediated via P2Y2 receptor

82 rved in the presence of CTP followed by ATP, UTP, and GTP.

83 possesses one major binding region for ATP, UTP, and GTP that partially overlaps with a previously d

84 ted with several nucleotides, including ATP, UTP, and ADP, in the presence or absence of selective in

85 nzoyl ATP (BzATP), alpha,beta methylene ATP, UTP, 2-methylthioATP (MeSATP), and ATPgammaS increased [

86 Mismatched ATP, UTP, or CTP could mediate excision of 3'-terminal CMP to

87 fied that is activated via nucleotides (ATP, UTP) binding apical P2 receptors and increasing [Ca(2+)]

88 Purinergic agonists (ATP/UTP) stimulated release of accumulated mucins in the Mun

89 tudies indicate a key role of the apical ATP/UTP-P2Y(2)-receptor system in the inhibition of ENaC P(o

90 nergic receptor subtypes, the Gq-coupled ATP/UTP-sensing P2Y2 receptor and the Gs-coupled A2b adenosi

91 ivation of P2Y (most likely, endothelial ATP/UTP-selective P2Y(2)) receptors, rather than via its dep

92 do not express CD39, that do not express ATP/UTP receptor P2Y2, and in controls.

93 We then identified the ATP/UTP receptor P2Y(2) as a critical sensor of nucleotides

94 ucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) reactivity and activation

95 ucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL)-positive cells were detect

96 ucleotidyl transferase-mediated biotinylated UTP nick end labeling assay revealed substantial deterio

97 ucleotidyl transferase-mediated biotinylated UTP nick end labeling positive, and they can be mitotica

98 ucleotidyl transferase-mediated biotinylated UTP nick end labeling) positive or apoptotic nuclei.

99 ucleotidyl transferase-mediated biotinylated UTP nick end labeling) staining in the cortex.

100 ucleotidyl transferase-mediated biotinylated UTP nick end labeling, cleaved caspase-3, and p53.

101 ucleotidyl transferase-mediated biotinylated UTP nick end labeling, hallmarks of apoptosis, were seen

102 ucleotidyl transferase-mediated biotinylated UTP nick end labeling-positive matrices and p53 at the o

103 ucleotidyl transferase-mediated biotinylated UTP nick end labeling-positive nuclei increased dramatic

104 ucleotidyl transferase-mediated biotinylated UTP nick end labeling.

105 ted from P2Y(2)R knockout mice restored both UTP-induced spreading and migration.

106 Thus, both UTP- and iNOS-generated reactive species contribute to E

107 stacks against the uracil base of the bound UTP, which on its other face also stacks with an essenti

108 The structure of p48 with bound UTP and Mn(2+) provides insights into the mechanism of n

109 Brain UTP, CTP, and CDP-choline were all elevated 15 min after

110 human (h) P2Y receptors (P2YRs) activated by UTP (P2Y2/4R) or UDP (P2Y6R).

111 to the nucleus continues to be activated by UTP by a mechanism dependent on Src kinase activity.

112 F109203x and Go6983 blocked BK activation by UTP in control epithelia, suggesting that PKC-mediated p

113 lcholine and phosphatidylethanolamine and by UTP-mediated activation of P2Y receptors.

114 coronary artery endothelial cells (HCAEC) by UTP/ATP increased the mRNA level of TF but not of its co

115 reversed P2X3 current inhibition induced by UTP-sensitive P2Y receptor activation.

116 bited by CTP and can be further inhibited by UTP, although UTP alone has little or no influence on ac

117 The feedback inhibition of uridine kinase by UTP and CTP in cell-free extracts was alleviated by incr

118 One of the operons regulated (in part) by UTP-sensitive reiterative transcription in E. coli is th

119 Activation of epithelial P2Y(2) receptors by UTP generated two patterns of [Ca(2+)](i) change: 2-10 m

120 2+ uptake and reduced stored Ca2+ release by UTP (400 microM) that activates a different family of in

121 ay, partially inhibited sAPPalpha release by UTP, whereas inhibitors of Src-dependent epidermal growt

122 -fluoromethylenetriphosphate (beta,gamma-CHF-UTP, 1) by (19)F NMR under conditions we previously pres

123 Under current clamp, UTP increased action potential (AP) firing in response t

124 ma-methylene substitution on the non-cognate UTP/dT scaffold ( approximately 3-fold decrease in kpol)

125 eractions alone are not sufficient to confer UTP selectivity.

126 In contrast, UTP-induced spreading and migration did not occur in SMC

127 karyotic UDP-GlcNAc biosynthesis, converting UTP and N-acetylglucosamine-1-phosphate (GlcNAc-1P) to U

128 In addition, CTP, UTP and nearly all UDP-activated sugars that serve as do

129 el the interactions of myosin with ATP, CTP, UTP, aza-ATP, ITP, and GTP (in decreasing order of effec

130 lates GpppA, GDP, and dGTP but not ATP, CTP, UTP, ITP, or m(7)GTP.

131 r activity than ATP, PSTK utilizes GTP, CTP, UTP and dATP as phosphate-donors.

132 leotide substrates, including ATP, GTP, CTP, UTP, and NAD.

133 as well as other nucleotides, including CTP, UTP, and GTP, had no effect on topoisomerase I cleavage

134 des has determined that Tlc4 transports CTP, UTP, and GDP.

135 estabilization of the R state by CTP and CTP/UTP, consistent with the T and R state crystallographic

136 Addition of CTP or the combination of CTP/UTP to the substrates significantly decreased the rate o

137 l-Lys-Arg-chloromethylketone also diminished UTP-induced sAPP alpha release.

138 Enolase, UTP-glucose-1-phosphate uridylyltransferase and polygala

139 djacent, does not coincide with the expected UTP binding site in apo Escherichia coli CTPS, suggestin

140 gulation of HAS2 expression by extracellular UTP, which is likely to contribute to the previously rep

141 explored the possibility that extracellular UTP or its breakdown products UDP and UMP act as mediato

142 Enzyme activity requires NTP, favors UTP, is stimulated by calcium, and initially produces 4

143 e relative fraction and sizes of fluorescent UTP-labeled transcripts in the nucleoplasm.

144 tance attributable to a reduced affinity for UTP.

145 on state for bond formation and cleavage for UTP/dT incorporation compared with ATP/dT incorporation.

146 compounds: choline, uridine (a precursor for UTP, CTP, and CDP-choline), and a PUFA (e.g., docosahexa

147 establish multicolor labeling strategies for UTP-enriched transcription compartments (TCs) and probe

148 Furthermore, UTP did not induce PLCgamma1 phosphorylation, and the ph

149 Furthermore, UTP particularly blocked the G1/S transition of GC cells

150 Furthermore, UTP selectively induces ICAM-1 expression in WT but not

151 Levels of ATP, ADP, ADP-glucose, UTP, UDP, and UDP-glucose were altered in a light-depend

152 secretion response was BzATP >> ATP > ADP >> UTP, and removal of external Ca(2+) dramatically suppres

153 t with lesser efficiency (ATP >> CTP > GTP > UTP).

154 It can hydrolyze ATP, GTP, UTP, and CTP.

155 binds 40S subunits and hydrolyzes ATP, GTP, UTP, and CTP.

156 rthermore, analog nucleotides including GTP, UTP and CTP, do not show serious interferences and this

157 icked by other Mg-nucleotides including GTP, UTP, AMP-PCP and ATP-gamma-S, but not by PP(i) or AMP, s

158 de uniporter Npt2(Ct), which transports GTP, UTP, CTP, and ATP.

159 ed with no observed [(3)H]adenosine or [(3)H]UTP transport.

160 The higher UTP values might be related to elevated UDP-glucose/gala

161 To determine how UTP is able to synergistically inhibit ATCase in the pre

162 flumic acid) and agonists (Forskolin + IBMX, UTP).

163 sociated temporally with a 20-nM increase in UTP and ATP content of bronchoalveolar lavage fluid, hyp

164 ATP and other nucleotides, including UTP, decrease ENaC activity via apical P2Y2 receptors.

165 into a (U,G)-adding enzyme that incorporates UTP and GTP instead of CTP and ATP; we transformed the r

166 to known ligands of the EGFR did not inhibit UTP-induced EGFR phosphorylation.

167 and silencing of ErbB3 expression inhibited UTP-induced phosphorylation of both ErbB3 and EGFR.

168 gene expression can be regulated by internal UTP levels, which reflect the availability of external p

169 n concomitant depletion of the intracellular UTP and CTP pools.

170 Intraventricular UTP injection transiently decreased blood flow monitored

171 In addition to established invariant UTP-binding determinants, we have identified and verifie

172 This is consistent with a binding step (K(UTP)=17+/-6 muM) followed by a conformational change (k=

173 e demonstrate functional conservation of key UTP-binding and metal-ion-coordinating residues and iden

174 (CaCC) is activated by Ca(2+) agonists like UTP.

175 We found that uridine triphosphate-mediated (UTP-mediated) Cl(-) secretion was reduced during the per

176 We found that uridine triphosphate-mediated (UTP-mediated) Cl- secretion was reduced during the perio

177 patterns of [Ca(2+)](i) change: 2-10 microm UTP induced [Ca(2+)](i) oscillations, whereas 100 microm

178 [Ca(2+)](i) oscillations, whereas 100 microm UTP induced a sustained [Ca(2+)](i) increase, both in th

179 wth factor 1 (IGF-1)-inducible mitochondrial UTP carrier (PNC1/SLC25A33) promotes cell growth.

180 10-100 mum UTP strongly up-regulated HAS2 expression, although the

181 ty and can cut DNA with GTP and CTP (but not UTP).

182 1 cells with A77-1726 (20 microM), a de novo UTP synthesis inhibitor, and 1400W completely reversed t

183 sP4 is unable to transfer other nucleotides (UTP, CTP, GTP, and dATP) to the acceptor RNA in the abse

184 ngement allows for independent adaptation of UTP and CTP binding affinities while efficiently utilizi

185 Efflux was induced with the addition of UTP (100 microM, 10.2 +/- 1.5 nmol min(-1)), which was b

186 scratch wounds or stimulation by addition of UTP caused a brief internalization of EGFR, which parall

187 The application of UTP (P2Y2 and P2Y4 agonist) sensitized colonic sensory n

188 er, these results explain how the binding of UTP can enhance the binding of CTP and why UTP binds mor

189 uggest the transient compartmentalization of UTP-enriched aggregates and their dynamic reorganization

190 of a prereaction ternary complex composed of UTP, TbTUT4, and UMP, which mimics an RNA substrate, and

191 By lowering the concentration of UTP needed for the initiation of RNA replication, CRE-de

192 ing that the intracellular concentrations of UTP and CTP may precisely regulate hCTPS activity.

193 ted with increased urinary concentrations of UTP and the ATP hydrolytic product, ADP.

194 As reported herein, low concentrations of UTP did not support negative-strand RNA synthesis when C

195 lls, are sensitive to high concentrations of UTP or CTP.

196 is of SG RNA at the lowest concentrations of UTP/CTP and that the single SVcpc mutation enhances the

197 The facilitatory effect of UTP on P2X(2) sustained currents was mediated by a G-pro

198 receptor/PKC pathway, whereas the effect of UTP on P2X(3) fast currents was G-protein independent.

199 These effects of UTP on bladder neuron excitability were blocked by the P

200 m BAPTA-AM completely blocked the effects of UTP on K(+) channel activation, indicating that the apam

201 erminal phosphate group into gamma-esters of UTP analogues.

202 th the templating base with the exception of UTP opposite purine deoxyribonucleoside.

203 ried in a hyperbolic manner as a function of UTP concentration.

204 rescence-based assay measuring inhibition of UTP-induced intracellular calcium release in 1321N1 astr

205 anisms in which high intracellular levels of UTP promote reiterative transcription that adds extra U

206 pendent VPg uridylylation lowers the K(m) of UTP required for viral RNA replication and that CRE-depe

207 respectively, reveal an unusual mechanism of UTP selection and domain organization previously unseen

208 1/59,000, 1/135,000 for misincorporation of UTP, ATP, and CTP opposite CMP in the template, respecti

209 te toward T while the additional presence of UTP makes the steady state vanishingly short.

210 ATCase is proposed in which the presence of UTP stabilizes the T state even more than CTP alone.

211 tetraphosphate and to a reduced response of UTP.

212 The EGFR-dependent stimulation of UTP-induced ERK1/2 phosphorylation in HSG cells is inhib

213 strong response to methylene substitution of UTP.

214 ns of pyrazofurin (inhibits the synthesis of UTP and CTP) were added to SVpzf-infected cells, the yie

215 phorbol ester pre-treatment had no effect on UTP-stimulated sAPP alpha release, indicating a PKC-inde

216 Stopped-flow kinetic studies on UTP binding followed by UMP incorporation into an EC as

217 results also indicate that release of ATP or UTP across the apical or basolateral membrane elicits qu

218 ryl acceptors, dCK can utilize either ATP or UTP as phosphoryl donors.

219 focal abluminal application of either ATP or UTP at the downstream end of cannulated arteries evoked

220 (HCAEC) and that P2Y2R activation by ATP or UTP induces dramatic up-regulation of tissue factor (TF)

221 Exposure to ATP or UTP inhibited AML-cell migration in vitro.

222 mpaired by apyrase and facilitated by ATP or UTP.

223 s of initiating nucleotides GTP, ATP, CTP or UTP to bind.

224 eferred substrate compared with GTP, CTP, or UTP.

225 nreactive with GDP, GTP, GpppA, ATP, CTP, or UTP.

226 Addition of ADP, GTP, or UTP to culture medium elevated the ATP concentration.

227 dATP, and ATP-agarose, but not CTP, GTP, or UTP, and exhibits ATPase activity.

228 and reduced AA release after thapsigargin or UTP treatment with decreased ERK1/2 and cPLA(2) phosphor

229 label from [alpha-(32)P]CTP or [alpha-(32)P]UTP into a RNase-sensitive and DNase-resistant product w

230 -(32)P]ATP and the formation of [gamma-(32)P]UTP is monitored by high-performance liquid chromatograp

231 verall conversion of UDP-Gal to [gamma-(32)P]UTP was linear between 0.5 and 30 nM UDP-Gal.

232 P2Y2 (UTP/ATP) and P2Y6 [ADP/UTP/uridine 5'-diphosphate (UDP)]

233 ow that UL84 has NTPase activity, preferring UTP.

234 , chelation of intracellular Ca(2+) prevents UTP-stimulated increases in diacylglycerol at the Golgi.

235 ding, subunit oligomerization and processive UTP incorporation, and predict druggable pockets.

236 d in the presence of the substrates/products UTP and UDP-glucose to nominal resolutions of 1.64 Angst

237 exhibited a >60% reduction in purinoceptor (UTP)-regulated CaCC activity.

238 he triphosphate moiety overlaps the putative UTP triphosphate binding site, explaining how CTP compet

239 idyltransferases that specifically recognize UTP and belong to a large enzyme superfamily typified by

240 RNAi knockdown of AC1 selectively reduced UTP-induced cAMP elevation and chloride secretion.

241 ntroduction of FLNa in FLNa RNAi SMC rescued UTP-induced LTA expression.

242 idered as contributing to the small residual UTP response.

243 tol-4,5-bisphosphate (PIP(2)) fully reverses UTP-mediated regulation of P2X3 channel activity.

244 The effects of several UTP analogs on the RdRp activity of the norovirus and fe

245 ng the arrangement of U3 RNA and the several UTP complexes that form a chaperone-like structure aroun

246 13-dibutyrate (PDBu) or a natural stimulant, UTP, time lapse live cell imaging movies indicated phosp

247 the K(m) and IC(50) values for the substrate UTP and the product CTP, respectively, were close to the

248 g that, although they are poorer substrates, UTP and GTP can also be utilized by Ssl2.

249 tocellular apoptosis as assessed by terminal UTP nick-end labeling when compared to ConA-treated Wt m

250 Here, we show by X-ray crystallography that UTP binds to a unique site on each regulatory chain of t

251 We found that UTP depleted PIP(2) abundance in the apical membrane whi

252 In addition, we found that UTP-stimulated LTA secretion is not sensitive to brefeld

253 munoprecipitation experiments indicated that UTP causes association of the EGFR with another member o

254 Agonist studies revealed that UTP, but not 2',3'-O-(4-benzoyl)benzoyl-ATP, 2-methylthi

255 answell cell migration assay, we showed that UTP significantly increased SMC spreading on collagen I

256 The UTP-stimulated iodide efflux was shown to be Ca(2+) depe

257 Specific siRNAs against the UTP receptor P2Y2, and inhibitors of UDP receptors P2Y6

258 As the UTP/CTP concentrations were increased, the SVpzf system,

259 ed knock-in phenotypes, we have assessed the UTP and RNA binding sites in RET2.

260 shift of conserved cationic Arg3.29 from the UTP gamma position to the delta position of Up4U and Up4

261 ing the involvement of these pathways in the UTP-induced HAS2 response.

262 44 and 435 positioned in the vicinity of the UTP binding site are critical for RET2 activity on singl

263 f the triphosphate moieties with that of the UTP substrate.

264 viously determined crystal structures of the UTP-bound and apo forms of the minimal trypanosomal TUTa

265 red viral RNA synthesis as a function of the UTP/CTP concentrations.

266 ncing of hBest1 in CFPAC-1 cells reduced the UTP-stimulated iodide efflux by around 40%.

267 n, thereby creating a cavity adjacent to the UTP-binding site.

268 and highly ordered water molecules with the UTP's base, ribose and phosphate moieties.

269 ological effects and metabolic conversion to UTP analog are L-ala,SP prodrug-dependent in cardiomyocy

270 Y6 and P2Y14, indicated that the response to UTP was mediated mainly through P2Y2 and to a lesser ext

271 In response to UTP, phosphorylation of GolgiCKAR was sustained the long

272 ent with reduced afferent fiber responses to UTP and ADP in Na(v)1.9(-/-) mice.

273 y, and terminal deoxynucleotidyl transferase UTP nick-end labeling (TUNEL) staining of histologic sec

274 s) selectively bind uridine 5'-triphosphate (UTP) and catalyze the addition of uridine 5'-monophospha

275 cells stimulated by uridine-5'-triphosphate (UTP) and PMA, agonists inducing LB fusion with the PM, b

276 Docked uridine 5'-triphosphate (UTP) formed a nucleobase pi-pi complex with conserved Ph

277 dy we revealed that Uridine-5'-triphosphate (UTP) uptake in living cells labeled transcription-relate

278 ch as ATP, ADP, and uridine 5'-triphosphate (UTP), among others, may serve as extracellular signaling

279 ceptor activated by uridine-5'-triphosphate (UTP), which is widely expressed in the body, e.g., in in

280 rocortisone (HC) on uridine-5'-triphosphate (UTP)-stimulated ion transport in differentiated, pseudos

281 tions in intracellular uridine triphosphate (UTP) and cytidine triphosphate (CTP).

282 Apical uridine triphosphate (UTP) stimulation was shown to increase short circuit cur

283 eas the other displays a more compact TUTase-UTP complex.

284 ws no appreciable conformational change upon UTP binding and apparently does not require RNA substrat

285 r (bp), fluorescein, and fluorescein-12-UTP (UTP) were separated in less than 120 s in channels of de

286 ; a pyrimidine (e.g., uridine, converted via UTP to brain CTP); and a PUFA (e.g., docosahexaenoic aci

287 gative-strand RNA synthesis, especially when UTP concentrations were limiting.

288 C0 conditions, activation only occurred when UTP was added after NS11021.

289 Whereas UTP is discriminated by 77,000-fold compared with dTTP,

290 f UTP can enhance the binding of CTP and why UTP binds more tightly in the presence of CTP.

291 Furthermore, stimulation of HSG cells with UTP induced phosphorylation of ErbB3, and silencing of E

292 nding site, explaining how CTP competes with UTP while CTP resistance mutations are acquired without

293 dition site) and mechanism (competition with UTP for occupancy of the NTP addition site) that differ

294 ET1 catalytic core alone and in complex with UTP analogs.

295 by EGF was attenuated by costimulation with UTP.

296 hosphorylated at the highest efficiency with UTP as donor.

297 f Grb2 when stimulated with EGF but not with UTP or injury.

298 e different kinetic properties observed with UTP to those with ATP.

299 te and the uridylyltransferase reaction with UTP to produce UDP-GlcNAc.

300 ed; and 3) secreted liquid when treated with UTP or forskolin or subjected to cyclic compressive stre