1  sheet RNA-binding platform, which mimics 5'-Ura-Gua-3' by making Watson-Crick-like hydrogen bonds wi

2 loop RNA resembles a molecular vise, with 5'-Ura-Cyt-Ade-Cyt-3' pinioned between an invariant Gly-X-X

3                                          All Ura(+) cells contained recombinant RNA3 replicons expres

4 nd B3Delta5' transcription was repressed and Ura(+) yeast cells were selected.

5                      Following selection for Ura- clones on 5-fluoroorotic acid containing medium, th

6 risomic strains grow well in the laboratory, Ura+ derivatives of CAI-4, carrying three copies of chro

7 er genes by repeated use of the URA3 marker (Ura-blaster methodology) has advanced our understanding

8                       Common applications of Ura-blaster technology result in different genomic posit

9 -tRNA (tRNA(Phe) containing FUra in place of Ura) to form a putative analog of a steady-state interme

10 ing genetic locations of URA3 as a result of Ura-blaster mutagenesis.

11 repair, and segregate to produce a sectored (Ura+/-) colony.

12 ro substituent activates the 6-carbon of the Ura heterocycle towards nucleophiles, and hence could pr

13 1-deficient mutant was achieved by using the Ura-blaster technique for sequential disruption of multi

14 A, is a potent competitive inhibitor of tRNA-Ura methyl transferase (RUMT).