ライフサイエンスコーパス: V region

1 ited by antibodies directed against the Scl1-V region.

2 ocuses pre-BCR-dependent selection on the HC V region.

3 re efficient AID deamination target than the V region.

4 vity to multiple residues located across the V region.

5 d motifs and, to some degree, throughout the V region.

6 ne contained a G2576U mutation in the domain V region.

7 V mutation in vivo and its targeting to the V region.

8 f its BCR to an idiotypic epitope in its own V region.

9 -kappa gene with an artificial insert in the V region.

10 start at CDR3 position four, well within the V region.

11 grafted into a nonhomologous human germ line V region.

12 iversity may be generated by mutation in the V regions.

13 antibody responses against a wide variety of V regions.

14 ed in combination with deletions of selected V regions.

15 ntral and peripheral T cell tolerance to BCR V regions.

16 ited cross-reactivity with heterologous TprK V regions.

17 sponse during infection is directed to these V regions.

18 paired somatic hypermutation (SHM) of the Ig V regions.

19 y specificity is a function of the variable (V) region.

20 ir generates mutations within immunoglobulin V-regions.

21 rgoes antigenic variation in seven variable (V) regions.

22 1 are found at "active" (DJ) and "inactive" (V) regions.

23 ty of antibodies sharing identical variable (V) regions.

24 n of different Env proteins, suggesting anti-V regions 1 and 2 Abs may be a marker of ADCC breadth.

25 FcgammaR-binding Abs specific to V regions 1 and 2 were strongly associated with increase

26 eterminants of Env, the CD4 binding site and V region 3, could in part account for the neutralizing a

27 itory receptor genes that encode a variable (V) region, a unique V-like C2 (V/C2) domain, a transmemb

28 r reactivity to patients' sera carrying anti-V region Abs to COL-1.

29 Looping of a subset of IgH V regions, albeit at lower frequency, was also observed

30 or excluded (V(-)) the alternatively spliced V region and contained either a mutated (H(-)) or an unm

31 immune repertoires including the entire IgH V region and enough of the IgH C region to identify isot

32 ay is regulated by the alternatively spliced V region and high-affinity heparin-binding domain of fib

33 w antisense transcription is specific to the V region and suggest transcripts extend across several g

34 s the CS-1 site on the alternatively spliced V region and the EIIIA region.

35 Idiotypes (Ids) are unique epitopes of Ab V regions and can trigger anti-Id immune responses, but

36 This interchangeability of TCR V regions and CDR3 motifs permits multiple structural so

37 vs, including gp160 or gp145 with or without V regions and gp41 deletions.

38 TCR beta- and alpha-chains showed identical V regions and invariant charged residues within the CDR3

39 of physical specificity between TCR germline V regions and MHC.

40 sively contained somatically mutated lambda1 V regions and were capable of producing Ag-specific Ab-f

41 The mutations were limited to V regions and were localized in known hotspots.

42 d in part by its syndecan-specific variable (V) region and in part by the second conserved (C2) regio

43 volved are specific to families of variable (V) regions and to some extent different rules may govern

44 s that the local sequence environment of the V region, and especially of the CDR1 and CDR2, is highly

45 BCRs, insufficient adaptive mutations in Ab V regions, and conformational instability of gp120.

46 eterogeneity is localized to seven variable (V) regions, and tprK sequence diversity accumulates with

47 ame is localized in seven discrete variable (V) regions, and variability results from apparent base c

48 he exact profile of rearrangement within the V region appears to be V gene specific.

49 These V regions are identical to those recently found in a reg

50 The TprK V regions are the focus of anti-TprK antibodies arising

51 These V regions are the targets of the host humoral immune res

52 body and have deimmunized both the variable (V) regions as well as the junction between the heavy (H)

53 athway by which microbial toxins that target V region-associated BCR sites induce programmed cell dea

54 ranslocations, and share features typical of V-region-associated somatic hypermutation.

55 argue that thymic selection shapes TCR-alpha V region bias in the preimmune repertoire; however, Ag i

56 tructural constraints imposed by C region on V region binding.

57 paring codons of CDR and FW of the germ-line V regions both to each other and to control regions.

58 ally matured versions of a type II variable (V) region, both in the presence and absence of its antig

59 of the different genes encodes an N-terminal V region but differs in the number of extracellular Ig d

60 dogenes are not evenly spread throughout the V region, but rather cluster together.

61 ucing mutations templated by adjacent pseudo-V regions, but impairment of gene conversion switches mu

62 e to MHC binding by other TCRs using related V regions, but not usually so dominantly.

63 ving exceptionally long CDR H3 loops and few V regions, but the mechanism for creating diversity is n

64 contrast, D47 and D19 IgG, sharing the same V regions, but which were not transcytosed by the pIgR,

65 and we propose a model for generation of new V regions by segmental gene conversion.

66 New sequences were observed in all seven V regions by the fifth slow passage.

67 that frequently result in the attachment of V region carbohydrate.

68 four criteria--the activities of N-cadherin/V region chimeras, syndecan-1 deletion mutants, or synde

69 the immunoglobulin heavy-chain variable (Igh-V) region compared with the constant region and partiall

70 t immunogen binding to Abs in their germline V region configuration expressed as BCRs, insufficient a

71 stricting flexibility, maintains a favorable V region conformation to allow superagonistic activity.

72 ontacts compared with wild-type, yet the TCR V-region contacts with MHC are conserved.

73 V region-containing chitin-binding proteins (VCBPs) cons

74 V region-containing immune-type genes constitute a far m

75 s of mouse-human chimeric Abs with identical V regions demonstrate differences in fine specificity an

76 These activities correlate with a V-region-dependent incorporation of cell-surface syndeca

77 B cells can present V region-derived Id peptides on their MHC class II molec

78 and 3, both germline and somatically matured V regions displayed significant structural changes in th

79 Chicago strain of T. pallidum and confirmed V region diversification during passage of this isolate.

80 V region diversity, particularly in V6, accumulates more

81 istinct B cell subpopulation with limited Ig V region diversity.

82 long RNA, comprising loop A, helix II, helix V, region E and helix IV, bound zf4-7 with high affinity

83 ucleotide long RNA, comprising loop A, helix V, region E and helix IV, but lacking helix II, retains

84 The demonstration that the V regions elicit a variant-specific antibody response su

85 as diverse, with five of six MAb heavy-chain V regions encoded by distinct members of the J558 family

86 mutated heavy chain immunoglobulin variable (V) region encoded by IGHV1-69, IGHD3-16, and IGHJ3 with

87 Focused libraries are built using V regions encoding combinations of canonical structures

88 ion, antibodies developed against all of the V regions except V1 and V3.

89 one, antibodies developed against all of the V regions except V1, while antibodies developed against

90 us, multigenic families encoding diversified V regions exist in a species lacking an adaptive immune

91 n the primary structure of the H and L chain V regions exist, the possibility that this level of rest

92 cells generated via preassembled IgH and IgK V region exons (HL).

93 assembly and subsequent selection of TCRbeta V region exons during thymocyte development.

94 Combinatorial assembly of IgH and IgL V region exons from gene segments generates preimmune Ig

95 utations that arose in the IGHV3-23*01 human V region expressed in vivo by human memory B cells revea

96 e initial immune response raised against the V regions expressed in the inoculum.

97 In summary, V-region expression in the context of the constant (C) r

98 Clonal evolution of Ig V regions, expression of activation-induced cytidine dea

99 se includes assignments to the IMGT germline V regions for heavy and light chains for several species

100 The average clone had 37 mutations in the V region, for a frequency of 0.11 mutations/base.

101 erantigens, which can interact via conserved V region framework subdomains of the Ag receptors of lym

102 gion heavy chain (V(H)) clan III genes via a V region framework surface that has been highly conserve

103 transcription cofactor Spt5 and AID, in the V region from germinal center cells, which is totally ab

104 ructed by domain swapping, which contain the V region from Scl1 proteins, were able to bind to ApoB10

105 generated phage-displayed libraries of IgNAR V regions from an immunized animal and found a family of

106 Sequencing of V regions from CD138(+) cells in MS CeSF has revealed so

107 nst synthetic peptides representing the TprK V regions from each clone.

108 ulin heavy chain (IgH) locus is critical for V region gene assembly.

109 TCR beta-chain V region gene diversity was determined by sequencing.

110 Our results reveal a subtle tier of V region gene evolution in which DNA sequence has been m

111 ssion through analysis of the TCR beta-chain V region gene products expressed in samples obtained fro

112 se collection of heavy-chain and light-chain V region gene products to form specific paratopes, with

113 Secondary Ab V region gene segment rearrangement, termed receptor edi

114 TRBV2 (formerly BV22) were the most expanded V region gene segments in DR3(+) LS patients relative to

115 equences of rearranged heavy and light chain V region gene segments in single cells from developing D

116 arising from the use of common H and L chain V region gene segments that share CDR3 structural featur

117 An immune network that has limited V region gene usage likely exists in the CSF and central

118 We studied the V region gene use in murine anti-MPO Abs derived from Sp

119 The variable (V) region gene encoding the heavy (H) chain underwent V-

120 This enables a single variable (V) region gene to be used in conjunction with different

121 Variable (V) region gene usage was diverse, with five of six MAb h

122 We sequenced Ab H and L chain V region genes (V(H) and V(L)) of clones expanded from s

123 study the selection of individual Ab H chain V region genes (V(H)), we performed CDR3 spectratyping o

124 th aberrant AID expression in Ph(+) ALL, IGH V region genes and BCL6 were mutated in many Ph(+) but u

125 Sequences of these V region genes demonstrated that each hybridoma expresse

126 led to productively rearrange immunoglobulin V region genes encoding a functional B-cell receptor (BC

127 studying nonproductive (hence unselected) Ig V region genes for somatic mutations and processed pseud

128 finding that the framework 2 region of kappa V region genes is highly mutable despite its importance

129 Analysis of the rearranged H chain V region genes of mAbs isolated from seven of these wome

130 The TCR gamma translocon contains at least 5 V region genes, 3 J segment genes, and 1 C segment.

131 s are not distributed randomly throughout Ab V region genes.

132 crease occurring in cases with unmutated IgH V region genes.

133 ll development, the immunoglobulin variable (V) region genes are subject to somatic hypermutation in

134 Ig variable (V) region genes are subjected to a somatic hypermutation

135 bstitutions into immunoglobulin variable (Ig V) region genes at all four bases, but the mutations at

136 d immunoglobulin heavy chain (IGH) variable (V) region genes devoid of somatic mutations.

137 atic mutation of immunoglobulin variable (Ig V) region genes is an important prognostic indicator of

138 that codon usage in immunoglobulin variable (V) region genes is such that the sequence-specificity of

139 Somatic mutation in immunoglobulin variable (V) region genes occurs largely in the germinal center an

140 of heavy and light chain germ line variable (V) region genes to form pneumococcal capsular PS (PPS) 6

141 the rearranged immunoglobulin (Ig) variable (V) region genes, and class-switch recombination (CSR) al

142 ypermutation (SHM) of the antibody variable (V)-region genes.

143 dominantly immunoglobulin G, were encoded by V-region genes expressed late in development, and displa

144 ng clonal relatives or by usage of different V-region genes.

145 Nonetheless, the precise role of this V region glycan remains unclear.

146 These results suggest that inappropriate V region glycosylation could contribute to ineffective A

147 nce analysis of B-1a, B-1b, and B-2 cell IgH V region H chain (V(H)) genes revealed increased usage o

148 ow passages (30- to 35-day intervals), three V regions had sequences that were completely different f

149 The BCR V region has been implicated as a potential avenue of T

150 cytidine deaminase targets the DNA encoding V regions, has enabled the analysis of its targeting pro

151 ain variable fragment (scFv) comprising both V region heavy chain (VH) and V region light chain (VL)

152 fold above normal, are targeted primarily at V-region hot spots by unknown mechanisms.

153 In contrast to mutations in V regions, however, these mutations are not detectable i

154 onse, B cells undergo Ab class switching and V region hypermutation, with the latter process potentia

155 more, isotype affected the polyreactivity of V region identical antibodies, implying a role for C reg

156 (V) region to affect paratope structure in a V region identical IgG(1), IgG(2a), IgG(2b), and IgG(3)

157 is study we compared the fine specificity of V region-identical IgE and IgA to Cryptococcus neoforman

158 leave the peptide at a much faster rate than V region-identical IgG subclasses and IgA, consistent wi

159 namic properties of the interactions of four V region-identical monoclonal antibodies with a univalen

160 hypermutation (ac-Nglycs) within Ig H chain V region (IGHV) genes as alternative selective pressures

161 using high-throughput sequencing of the IgH V region (IGHV) genes, we have studied features of TG2-s

162 The IgH V region (IGHV) is central to Ag binding and consists of

163 d-type cells, substitution of a heterologous V region impaired the survival of three ABC lines.

164 o demonstrate functional roles of syndecan-1 V region in laminin-dependent C2C12 cell adhesion and th

165 eptides derived from the immunoglobulin (Ig) V region in some but not all patients.

166 animal indicating the functionality of this V region in the context of immune defense against pathog

167 demonstrate the dominant role of the H chain V region in TSHR recognition.

168 found that RNA polymerase II accumulates in V regions in B cells after both types of stimulation for

169 The usage of TCR alpha and beta V regions in the cell line was oligoclonal.

170 e the possibility that expression of certain V regions in the context of alpha and C regions affects

171 ue to the failure to utilize the appropriate V regions in the pre-immune repertoire.

172 be a global defect in T cell tolerance to Ab V regions in this autoimmune-prone strain either before

173 his study, we searched for ssDNA in mutating V regions in which DNA-protein complexes were preserved

174 K (TprK) differs in seven discrete variable (V) regions in isolates and that the antibody response du

175 LE in which all somatic mutations within ANA V regions, including those in CDR3, could be unequivocal

176 pecificity is solely the result of variable (V)-region interactions with an antigen.

177 However, monoallelic looping of IgH V regions into close proximity of the IgH DJ cluster was

178 eable TAT-V peptide--we demonstrate that the V region is necessary and sufficient for these cell beha

179 nlike Ig genes, somatic hypermutation of TCR V regions is an infrequent event.

180 tained when a peptide from the BCR variable (V) region is expressed by small numbers of B cells as it

181 w the somatic mutation of antibody variable (V) regions is generated.

182 We confirmed these findings in an analogous V region knock-in mouse and/or in non-Tg mice.

183 a cells secrete a monoclonal Ig containing a V region L chain (VL) epitope recognized by CD4+ T cells

184 omprising both V region heavy chain (VH) and V region light chain (VL) of mAb Z22 and that the VH dom

185 CD8(+) T cells recognized overlapping third V region loop peptides.

186 rface Ig is mandatory and carries a striking V-region modification because of introduction of glycan

187 The results suggest that V-region motifs associated with annular binding and opso

188 V region mutation hotspots are largely determined by AID

189 allenging assumptions about the location and V region mutation status of memory cells.

190 s exhibit 5-10-fold increases in heavy-chain V-region mutations targeted only predominantly to RGYW (

191 shows selection for rearrangement within the V region of a number of genes and for CD8 and CD4 cells.

192 ccurring somatic mutations in the Ab H chain V region of Fab19, a well-described neutralizing human m

193 Taken together, the V region of human anti-6B antibodies is commonly formed

194 Somatic hypermutation (SHM) diversifies the V region of Ig genes and underlies the process of affini

195 ed carbohydrates are frequently found in the V region of Ig H chains and can have a positive or negat

196 e have tested the hypothesis that the unique V region of syndecan-1 cytoplasmic domain has a crucial

197 haviors that depend on signaling through the V region of syndecan-1.

198 nce is caused by mutations within the domain V region of the 23S ribosomal RNA (rRNA) gene, which is

199 A 420-bp domain V region of the 23S rRNA gene from all isolates was ampl

200 presence of a T2500A mutation in the domain V region of the 23S rRNA gene.

201 act to immunogenic peptides derived from the V region of the BCR that are created by somatic mechanis

202 ation-induced cytidine deaminase mutates the V region of the Ig genes to increase the affinity of Abs

203 ic and intergenic, occurs extensively in the V region of the immunoglobulin heavy chain locus.

204 Furthermore, the non-collagenous V region of the Scl1 protein is responsible for LDL/ApoB

205 that differed solely with respect to the IgH V region of their BCRs.

206 oo contain an amino-terminal non-collagenous V region of unknown function.

207 rated a double knock-in mouse that expresses V regions of a somatically mutated anti-Id mAb with inte

208 teins that interact with conserved motifs in V regions of B cell Ag receptor shared by large sets of

209 Analysis of somatic mutations in V regions of Ig genes is important for understanding var

210 quences that are preferentially found in the V regions of Ig genes.

211 tion of a human rIgA(2) mAb that carries the V regions of IgG1 b12, a potent and broadly neutralizing

212 e demonstrate that antibody responses to the V regions of one TprK molecule show limited cross-reacti

213 dues within the basic pocket and surrounding V regions of the B30.2 domain abrogated prenyl pyrophosp

214 Antibodies were highly specific for the V regions of the infecting clone, and cross-reactivity w

215 e describe the sequence anatomy of the seven V regions of tprK and the identification of putative don

216 for targeting AID activity to the variable (V) region of Ig genes are unknown.

217 The genes encoding the variable (V) region of the B-cell antigen receptor (BCR) are assem

218 of somatic hypermutation (SHM) of variable (V) regions of Ig genes.

219 Instead, the variable (V) regions of IgNAR bind antigen as soluble single domai

220 genomic DNA sequencing of multiple variable (V) regions of the bacterial 16S ribosomal gene, to inter

221 the possibility that looping of distinct IgH V regions plays a role in promoting long-range interacti

222 known about their genetics or the variable (V) region polymorphisms that affect their protective fun

223 Antibodies to the N-region or V-region polypeptides, but not antibodies to the rC-Msp

224 The N- and V-region polypeptides, but not rC-Msp, also bound to the

225 We determined complete V region primary structures and performed antigen bindin

226 -regulates Ig H chain production from select V region promoters and requires Bright dimerization, Bru

227 he results suggest that the C region affects V region protein conformation, leading to differences in

228 developed CLL clones with identical antibody V regions randomly is highly improbable and suggests sel

229 ydrate addition sequence generated by either V region rearrangement or somatic hypermutation may resu

230 traightforward, even when the same variable (V) region recognizes the same MHC molecule.

231 hat the diversity of the expressed variable (V) region repertoire of the immunoglobulin (Ig)H chain o

232 e ability of TCR chain pairing to modify how V region residues interact with pMHC helps to explain ho

233 to bind to its own cell surface depended on V region residues that mediate the intrinsic autoreactiv

234 To minimize anti-V region responses, a variant of HuCOL-1 was generated b

235 The V region-restricted histone hyperacetylation resulting f

236 Targeting of AID to antibody variable (V) regions results in somatic hypermutation, whereas its

237 rrangement frequency distribution within the V region reveals selection on CDR3 position four.

238 Light-chain V-region segments were derived from the Vk1, Vk4/5, Vk10

239 d passages (9- to 10-day intervals), no tprK V region sequence changes were observed.

240 assembled the entire 2.5-Mb mouse IgH (Igh) V region sequence of the C57BL/6 strain from public sequ

241 ective efficacy, presumably due to variable (V)-region sequence differences resulting from somatic mu

242 ted for approximately 70% of the IgG H chain V regions sequenced, while the corresponding IgM reperto

243 ammadelta cells and demonstrating that Vbeta V region sequences are not required for selection of an

244 This indicates that gene conversion between V region sequences can occur in mouse B cells; we propos

245 To test our hypothesis that V region sequences change during infection and passage,

246 ed strategy to analyze the repertoire of IgG V region sequences expressed in SSPE brain.

247 determine the distribution of TCR beta-chain V region sequences expressed in the transferred cells as

248 Sequence analysis of amplified IgG V region sequences identified the rearranged germline se

249 abbits may explain the limited repertoire of V region sequences seen in the Nichols strain.

250 The parent strain expresses many different V region sequences, and infection with this strain induc

251 entification of putative donor sites for new V region sequences, and we propose a model for generatio

252 rance in developing T cells specific for BCR V region sequences, thus ensuring that T cell help to B

253 ivation-induced cytidine deaminase acting on V-region sequences is sufficient to initiate authentic f

254 Somatic mutations in the IgNAR V region serve to increase the number of contacts with a

255 Sequence analysis of antibody V regions showed evidence of somatic and affinity matura

256 microbial Ig-binding protein specific for a V region site on Ig L chains.

257 ace during the secondary AFC response but Ab V region somatic hypermutation is not reinduced.

258 oducing mutations within the antigen-binding V regions (somatic hypermutation, SHM) and double-strand

259 V-region specificity forms through genetically programme

260 terodimeric configurations with other native V-region splice variants in cartilage.

261 es not efficiently heterodimerize with other V-region splice variants of fibronectin.

262 We conclude that the C region can modify the V region structure to alter the Ab paratope, thus provid

263 tributed the major variability in the intact V region structure.

264 affect binding affinity and specificity and V-region structure.

265 including those usually associated with the V region such as affinity for Ag as well as other charac

266 Three-dimensional models of the V regions suggested that residues that affect binding to

267 (A) and proline-rich (P) repeats flanking a V region that is projected distal from the cell.

268 sence in novel immune-type receptor genes of V regions that are related closely to those found in Ig

269 nhibit rearrangements to the gamma and delta V regions that normally recombine almost exclusively dur

270 We found that V regions that undergo SHM were enriched in short patche

271 somatic hypermutation (SHM) of Ig variable (V) regions that is required for the affinity maturation

272 ass switch serves to distribute a particular V region to different Ig C regions.

273 se that antisense transcription remodels the V region to facilitate V(H)-to-DJ(H) recombination.

274 conformational constraints on the variable (V) region to affect paratope structure in a V region ide

275 in (IgH) gene that allows the same variable (V) region to be expressed with any one of the downstream

276 CRs had an alpha-chain bearing the variable (V) region TRAV1-2 rearranged to the joining (J) region T

277 ne profiling and deep sequencing of TCR-beta V regions, two subsets of cTregs, based on expression of

278 Rearranged Ig V regions undergo activation-induced cytidine deaminase

279 Secondary diversification occurs when Ig V regions undergo somatic hypermutation (SHM) and affini

280 sm responsible for the formation of ssDNA in V regions undergoing SHM has yet to be experimentally es

281 treatment with a TLR9 agonist protected IgH V region unmutated, but not mutated, CLL cells from apop

282 blood, in the case of B-CLL with Ig H chain V region-unmutated BCR and <30% CD38(+) cells in blood.

283 murine IgG switch variants that differed in V region usage for Cryptococcus neoformans glucuronoxylo

284 IgH V regions used by the BCRs of ABC DLBCL biopsy samples v

285 cted mutagenesis of the MAb 12A1 heavy-chain V region (V(H)) was followed by serological and function

286 CSR) by deaminating cytidines to uridines at V region (V) genes and switch (S) regions.

287 V neutralizing murine IgM with H and L chain V regions (V(H) and V(L) regions) free of immunogen-driv

288 is, the TprK Ag undergoes variation of seven V regions (V1-V7) by nonreciprocal recombination of sile

289 "semi-composite" 16S profiles using multiple V regions validated by quantitative PCR analysis confirm

290 oxymethyl that forms a common TCR beta-chain V region variant.

291 ally done by interrogation of paired H chain V region (VH) and L chain V region (VL) sequences of ind

292 of paired H chain V region (VH) and L chain V region (VL) sequences of individual and Ag-specific B

293 n, the frequency of mutations throughout the V region was reduced.

294 n reported to be associated with light chain V regions, we have begun an analysis of germ line light

295 this, we found that both DNA strands in the V region were transcribed.

296 Expressed Ig V regions were amplified by RT-PCR.

297 ete and definitive assembly of the mouse Igh V region will facilitate detailed study of promoter func

298 e C region can affect the interaction of the V region with an Ag.

299 -specific clonotype expresses Valpha11Vbeta3 V regions with preferred sequence features in the third

300 somatic hypermutation of rearranged lambda1 V regions within secondary AFCs showed a strong correlat