ライフサイエンスコーパス: V. fischeri

1 V. fischeri ADP-r has no significant homology (DNA or am

2 V. fischeri colonizes the crypts of a host organ that is

3 V. fischeri culture media have lower osmolarities than a

4 V. fischeri has six flagellin genes that are uniquely ar

5 V. fischeri hnoX encodes a heme NO/oxygen-binding (H-NOX

6 V. fischeri qsrP and ribB mutants exhibited no distinct

7 llowing first exposure, only approximately 5 V. fischeri cells aggregated on the organ surface.

8 A V. fischeri mutant defective in chitin catabolism was ab

9 A V. fischeri mutant unable to produce PepN is significant

10 nization of juvenile E. scolopes; however, a V. fischeri strain lacking TMAO reductase activity displ

11 d the molecular biological construction of a V. fischeri halovibrin null strain.

12 lysaccharide, however, little is known about V. fischeri biofilm matrix components.

13 These updates further advance V. fischeri as an important model for understanding inte

14 Also, V. fischeri strains were markedly more successful than V

15 with the luciferases from P. leiognathi and V. fischeri.

16 ntain a mixed population of Vibrio logei and V. fischeri, with V. logei comprising between 63 and 100

17 Another V. fischeri gene, ainS, directs the synthesis of N-octan

18 RNA-Seq dataset representing host-associated V. fischeri cells from colonized juvenile E. scolopes, a

19 ame the apparent restriction barrier between V. fischeri and Escherichia coli.

20 portant insight into the interaction between V. fischeri and E. scolopes.

21 s an important role in the symbiosis between V. fischeri and its squid host.

22 ways important for symbiotic colonization by V. fischeri and establishes a paradigm for evaluating tw

23 s biofilm formation and host colonization by V. fischeri via its impact on transcription of the symbi

24 tivated to promote symbiotic colonization by V. fischeri.

25 etry demonstrated that oxygen consumption by V. fischeri CydAB quinol oxidase is inhibited by NO trea

26 ween OMV production and biofilm formation by V. fischeri.

27 environment to regulate biofilm formation by V. fischeri.

28 protein necessary for biofilm maturation by V. fischeri and, based on the conservation of bmp, poten

29 ed whether the net release of PG monomers by V. fischeri resulted from lytic transglycosylase activit

30 lete understanding of the matrix produced by V. fischeri to enhance cell-cell interactions and promot

31 for promoting colonization of E. scolopes by V. fischeri.

32 During the early stages of colonization, V. fischeri is exposed to host-derived nitric oxide (NO)

33 genes) was absent in over 97% of these dark V. fischeri strains.

34 These 'dark' V. fischeri strains remained non-bioluminescent even aft

35 We used a recently developed V. fischeri microarray to identify genes that are contro

36 high inocula demonstrated that environmental V. fischeri cells aggregate during a 3 h period in host-

37 4.3-Mbp genome sequence represents the first V. fischeri genome from an S. robusta symbiont and the f

38 d from the marine bacterium Vibrio fischeri (V. fischeri ADP-r) is described.

39 irectly and in real time, approximately five V. fischeri cells aggregate along the mucociliary membra

40 These results indicate a role for V. fischeri AOX in aerobic respiration during NO stress.

41 In a screen for V. fischeri colonization mutants, we identified a strain

42 nsible for this phenomenon, the lipid A from V. fischeri ES114 LPS was isolated and characterized by

43 Lipid A from V. fischeri, E. coli, or S. flexneri induced apoptosis.

44 A 606 bp open reading frame was cloned from V. fischeri that encoded a protein, which we named LitR,

45 we have cloned a gene, designated flrA, from V. fischeri that encodes a putative sigma(54)-dependent

46 clone a cross-hybridizing DNA fragment from V. fischeri genomic DNA.

47 in combination with L(H) or luciferase from V. fischeri (L(F)).

48 s induced three- to fourfold both as growing V. fischeri cells approach stationary phase and upon the

49 In V. fischeri, luxi directs the synthesis of N-(3-oxohexan

50 this model, we found that bioluminescence in V. fischeri ES114 is modulated by glucose and stimulated

51 A is the predominately expressed catalase in V. fischeri and indicating that V. fischeri carries only

52 ophore synthesis and symbiosis competence in V. fischeri that involves the glnD gene.

53 ify other regulators of biofilm formation in V. fischeri, we screened a transposon library for mutant

54 ibuted to RscS-mediated biofilm formation in V. fischeri.

55 ride loci contribute to biofilm formation in V. fischeri.

56 al strain, indicating that AI-2 functions in V. fischeri to delay luminescence induction.

57 AI-2 apparently functions in V. fischeri to suppress or delay induction at low and in

58 in modulating other symbiotic functions, in V. fischeri.

59 rge-scale mutagenesis of a class of genes in V. fischeri using a genomic approach and emphasizes the

60 ajor contributor to TMAO-dependent growth in V. fischeri under the conditions tested.

61 Genetic arrangement of the flrA locus in V. fischeri is similar to motility master-regulator oper

62 ited induction in a dose-dependent manner in V. fischeri and Escherichia coli carrying the lux genes.

63 Moreover, in V. fischeri, we observed ainR-dependent repression of a

64 onal regulation of TMAO reductase operons in V. fischeri appears to differ from that in previously st

65 regions of a number of flagellar operons in V. fischeri revealed apparent sigma(54) recognition moti

66 nnection between the Lux and Syp pathways in V. fischeri, and furthers our understanding of how the L

67 We report the presence in V. fischeri of ompU, a gene encoding a 32.5-kDa protein

68 ously identified several non-Lux proteins in V. fischeri MJ-100 whose expression was dependent on Lux

69 establishing that there is a LuxR regulon in V. fischeri MJ-100 whose genes are coordinately expresse

70 more fully characterize the LuxR regulon in V. fischeri MJ-100, real-time reverse transcription-PCR

71 cyl-HSL-responsive quorum-sensing regulon in V. fischeri.

72 ggest that the two quorum-sensing systems in V. fischeri, ain and lux, sequentially induce the expres

73 plication of molecular genetic techniques in V. fischeri.

74 ure for the introduction of plasmid DNA into V. fischeri by electroporation, and isolated a mutant st

75 istent with that activity, introduction into V. fischeri of medium-copy plasmids carrying these genes

76 ulates the aphrodisiac-like activity of live V. fischeri.

77 when colonizing the light organ of the model V. fischeri host, the Hawaiian bobtail squid Euprymna sc

78 We recently identified a novel V. fischeri locus, ainS, necessary for the synthesis of

79 lipopolysaccharide (LPS) or free lipid A of V. fischeri can trigger morphological changes in the juv

80 When grown in culture, cells of V. fischeri strain PMF8, in which litR was insertionally

81 e did not compromise symbiotic competence of V. fischeri; however, levels of colonization of an ainS

82 imal host and presents the first examples of V. fischeri genes that affect normal host tissue develop

83 er, these results show that the flagellum of V. fischeri is a complex structure consisting of multipl

84 nt, a galactose-utilization mutant (galK) of V. fischeri colonized juvenile squid to wild-type levels

85 rate here that the flavohaemoglobin, Hmp, of V. fischeri protects against NO, both in culture and dur

86 ing Euprymna scolopes, the symbiotic host of V. fischeri.

87 In this study, we investigated the impact of V. fischeri LuxS on luminescence and colonization compet

88 r this association, we searched a library of V. fischeri transposon insertion mutants for those that

89 We found the luminescence of V. fischeri to be correlated with external osmolarity bo

90 with a recently developed metabolic model of V. fischeri provides us with a clearer picture of the me

91 ring symbiosis onset and, (ii) modulation of V. fischeri growth in symbiotic maintenance.

92 cyclic di-GMP in the control of motility of V. fischeri.

93 Importantly, the acs mutant of V. fischeri has a competitive defect when colonizing the

94 A Deltahmp mutant of V. fischeri initiates squid colonization less effectivel

95 n to high density, we identified a mutant of V. fischeri that exhibited an apparent defect in symbios

96 ed and characterized a glnD:mTn5Cm mutant of V. fischeri.

97 Here, we show that the repressor NagC of V. fischeri directly regulates several chitin- and N-ace

98 Here, we asked whether OMVs are part of V. fischeri biofilms.

99 x genes, we examined the protein patterns of V. fischeri quorum-sensing mutants defective in luxI, ai

100 n aggregation, suggest a two-step process of V. fischeri cell engagement: association with host cilia

101 o significantly decreased the growth rate of V. fischeri in culture.

102 ays, we identified three novel regulators of V. fischeri luminescence and seven regulators that alter

103 light organ, in which case, the response of V. fischeri to NO is of considerable interest.

104 Here we report the genome sequence of V. fischeri ES114, which enters into a mutualistic symbi

105 n this system include the genome sequence of V. fischeri, an expressed sequence tagged library for E.

106 ent, we constructed a non-luminous strain of V. fischeri (delta luxA::erm).

107 iotic resistance marker to another strain of V. fischeri.

108 sest known relatives, flrA mutant strains of V. fischeri ES114 were completely abolished in swimming

109 ecular genetic techniques, mutant strains of V. fischeri have been constructed that are defective at

110 Through phenotypic studies of V. fischeri mutants we have found that the AinS-signal i

111 r NADase activity in culture supernatants of V. fischeri, and this mutant initiated the light organ s

112 To understand environmental influences on V. fischeri motility, we investigated migration of this

113 Like other gram-negative organisms, V. fischeri expresses lipopolysaccharide (LPS) on its ce

114 ing a chemoattractant gradient that promotes V. fischeri migration into host tissues.

115 Hmp protects V. fischeri from NO inhibition of aerobic respiration, a

116 Purified V. fischeri LPS, as well as the LPS of V. cholerae, Haem

117 e limitation or (ii) a mutation that renders V. fischeri defective in the synthesis of a homolog of t

118 experiments demonstrate that the E. scolopes-V. fischeri system is a viable model for the experimenta

119 re prone to later superinfection by a second V. fischeri strain.

120 Here, we show that a single V. fischeri protein, the previously uncharacterized EroS

121 symbionts, is absent from the fish symbiont V. fischeri MJ11.

122 In the squid symbiont V. fischeri ES114, RscS controls light-organ colonizatio

123 ntified TMAO reductase activity in symbiotic V. fischeri isolates associated with the light organs of

124 le the luminescence (lux) genes of symbiotic V. fischeri have been shown to be highly induced within

125 ast 9 aa to the growing culture of symbiotic V. fischeri present in its light-emitting organ.

126 We found that His-tagged V. fischeri CRP could bind sequences upstream of both lu

127 is a component of the host defense, and that V. fischeri uses a cytotoxin-like molecule to induce hos

128 This demonstrates that V. fischeri quorum sensing regulates a substantial numbe

129 luminescence autoinducer, demonstrating that V. fischeri makes no luminescence autoinducers other tha

130 ork thus reveals a novel group of genes that V. fischeri controls through a sigma54-dependent respons

131 Taken together, these data indicate that V. fischeri LuxS affects both luminescence regulation an

132 catalase in V. fischeri and indicating that V. fischeri carries only a single catalase gene.

133 ecular Microbiology, Dunn et al. report that V. fischeri produces an NO-inducible and NO-resistant al

134 emical analysis of this mutant revealed that V. fischeri hvn null still possessed ADP-ribosyltransfer

135 Finally, we show that V. fischeri, purified EroS, and other bacterial chondroi

136 The results suggest that V. fischeri may help modulate the host stress responses

137 Our data suggest that V. fischeri normally senses a host-generated NO signal t

138 active during colonization, suggesting that V. fischeri symbionts are exposed to NO.

139 The V. fischeri luxI mutant does not express detectable ligh

140 The V. fischeri mutant grew poorly with galactose as a sole

141 On addition of the acceptor, the V. fischeri yellow fluorescence protein containing eithe

142 essful colonization of E. scolopes, i.e. the V. fischeri ainS mutant failed to persist in the squid l

143 A null mutation introduced into the V. fischeri ompU resulted in the loss of an OMP with an

144 e but may also use this light to monitor the V. fischeri population.

145 We have investigated the impact of the V. fischeri acyl-HSL synthase AinS on both luminescence

146 trate in the luxI-dependent synthesis of the V. fischeri autoinducer.

147 Comparison of the V. fischeri ES114 genome with that of conspecific strain

148 y between E. coli Pgm and the product of the V. fischeri gene, which was therefore designated pgm.

149 Analysis of the V. fischeri genome revealed the presence of a putative t

150 e, it is possible that these features of the V. fischeri lipid A may underlie the ability of E. scolo

151 The expression of the V. fischeri OmpU was not significantly affected by eithe

152 tical issues concerning the synthesis of the V. fischeri signal.

153 In contrast, the luminescence of the V. fischeri symbionts is optimal above 24 degrees C and

154 s to the already considerable utility of the V. fischeri-E. scolopes model system.

155 e wild-type and waaL strains showed that the V. fischeri LPS has a single O-antigen repeat composed o

156 Our results show that the V. fischeri waaL mutant has a motility defect, is signif

157 moserine lactone structurally related to the V. fischeri and other autoinducers.

158 on the ainS promoter depended on whether the V. fischeri regulatory gene litR was also introduced.

159 nescent strains exhibit monophyly within the V. fischeri clade.

160 onent response regulators encoded within the V. fischeri genome.

161 Hmp, influence aggregate size and, thereby, V. fischeri colonization efficiency.

162 ts reduced the inhibition of luminescence to V. fischeri, i.e., were beneficial for the bacteria, wit

163 compared and analyzed the ain locus from two V. fischeri strains and a Vibrio salmonicida strain to e

164 a glycerol/tryptone-based medium, wild-type V. fischeri cells initially excrete acetate but, in a me

165 was localized in the periplasm of wild-type V. fischeri cells, where its role could be to detoxify h

166 predicted, in the presence of NO, wild-type V. fischeri grew more slowly on hemin than a hnoX deleti

167 evels relative to that achieved by wild-type V. fischeri.

168 These studies indicate that the unusual V. fischeri O-antigen sugars play a role in the early ph

169 This study lays the foundation for using V. fischeri as a model system for studying TMAO reductas

170 When V. fischeri cells were introduced into the seawater surr

171 This light organ symbiosis is initiated when V. fischeri cells present in the surrounding seawater en

172 ity by SypE is a critical mechanism by which V. fischeri controls biofilm development and symbiotic c

173 Thus, this work reveals a mechanism by which V. fischeri inhibits cellulose-dependent biofilm formati

174 o play a role in the normal process by which V. fischeri initiates its colonization of the nascent li

175 surrounding mucus, the environment in which V. fischeri cells aggregate before migration into the or

176 ent work suggests that the tissue with which V. fischeri associates not only can detect bioluminescen

177 similar to that triggered by infection with V. fischeri.

178 First, animals inoculated with V. fischeri aboard the space shuttle showed effective co

179 ymna scolopes forms a natural symbiosis with V. fischeri, and utilizes the symbiont-derived biolumine