ライフサイエンスコーパス: V. vulnificus

1 V. vulnificus infections were reported to the Centers fo

2 V. vulnificus inoculated into iron dextran-treated mice

3 V. vulnificus is a gram-negative bacterium, considered o

4 V. vulnificus is able to use host iron sources such as h

5 V. vulnificus strains containing pGTR902 were inoculated

6 V. vulnificus-associated diseases are noted for the rapi

7 Results showed that ca. 10(3) V. vulnificus bacteria/gram of oyster and higher concent

8 the rtxA1 gene that encodes MARTX(Vv) in 40 V. vulnificus Biotype 1 strains and found four distinct

9 In a survey of the 16S rRNA genotype in 67 V. vulnificus human clinical and nonclinical strains, we

10 We examined 69 V. vulnificus biotype 1 strains that were genotyped by s

11 thal factor or when naturally delivered as a V. vulnificus MARTX toxin led to loss of mitochondrial m

12 is study, we have cloned and characterized a V. vulnificus type IV pilin (PilA) that shares extensive

13 lated outer membrane protein purified from a V. vulnificus fur mutant had 53% homology with the first

14 Using TnphoA mutagenesis, we identified a V. vulnificus CPS locus, which included an upstream ops

15 sults discussed here confirmed homology of a V. vulnificus CPS locus to the group 1 CPS operon in Esc

16 Phenotypic characterization of a V. vulnificus vvpD mutant, constructed by allelic exchan

17 ilD) mutant of Pseudomonas aeruginosa with a V. vulnificus genomic library.

18 bacteriophages as therapeutic agents against V. vulnificus in an iron-dextran-treated mouse model of

19 hat estrogen is providing protection against V. vulnificus lipopolysaccharide-induced endotoxic shock

20 sular genetics and antigenic diversity among V. vulnificus strains.

21 Both V. vulnificus CPS and V. vulnificus LPS induced inflammation-associated cytoki

22 ered for orthologs of HapR in V. harveyi and V. vulnificus.

23 antibiotic-resistant V. parahaemolyticus and V. vulnificus, including V. parahaemolyticus pandemic st

24 ains), V. parahaemolyticus (30 strains), and V. vulnificus (10 strains) to determine the accuracy of

25 Both V. vulnificus CPS and V. vulnificus LPS induced inflamma

26 localized and systemic infections caused by V. vulnificus in animals.

27 t hospitalizations and deaths were caused by V. vulnificus infection, and most patients were white me

28 inflammation-mediated septic shock caused by V. vulnificus is strongly associated with liver disease,

29 odel for studying systemic disease caused by V. vulnificus.

30 erall levels of these molecules expressed by V. vulnificus isolates.

31 uction of the intrinsic apoptosis pathway by V. vulnificus.

32 septicemia in humans, secretes a PFT called V. vulnificus hemolysin (VVH), which contains a single C

33 hages were effective against three different V. vulnificus strains with various degrees of virulence,

34 However, two distinct V. vulnificus genotypes or alleles were associated with

35 road exopeptidase activity which may enhance V. vulnificus invasiveness by altering peptides involved

36 tant, containing the transposon and flanking V. vulnificus DNA was cloned, and a probe complementary

37 For V. vulnificus, the ability to acquire iron from the host

38 at but were not significantly attenuated for V. vulnificus virulence in mice.

39 n of fatty acid metabolism are essential for V. vulnificus to be able to cause disease in mammalian h

40 a niche marker, not always through Fur, for V. vulnificus controlling its entire life cycle.

41 Using the rat, we have developed a model for V. vulnificus endotoxic shock that mimics the sexually d

42 ndividuals that develop endotoxic shock from V. vulnificus are males.

43 , TcdA, TcnA, and TcsL; putative toxins from V. vulnificus, Yersinia sp., Photorhabdus sp., and Xenor

44 ransport genes are regulated by iron, and in V. vulnificus, transcriptional regulation by iron depend

45 The role of the capsule in V. vulnificus biofilms was examined under a variety of c

46 hat a wide variety of capsular carbotypes in V. vulnificus may be readily distinguished by the HPAE f

47 ociated with both CPS and rEPS expression in V. vulnificus, designated the wcr (capsular and rugose p

48 gene is essential for capsule expression in V. vulnificus.

49 that the HlyU protein, a virulence factor in V. vulnificus CMCP6, up-regulates the expression of VV20

50 n future studies of the role of heme iron in V. vulnificus pathogenesis.

51 xia, has yet to be extensively researched in V. vulnificus.

52 and the extracytoplasmic stress response in V. vulnificus, mutants with defined mutations in rseB an

53 or pilus assembly and cytolysin secretion in V. vulnificus.

54 or the function of the TtpC2-TonB2 system in V. vulnificus.

55 onfirm that phase variation and virulence in V. vulnificus correlate with the amount of CPS expressed

56 Interestingly, V. vulnificus infections disproportionately affect males

57 experiments in a murine model of intravenous V. vulnificus infection demonstrated that expression of

58 ences in virulence among naturally occurring V. vulnificus can be explained by diverse abilities to r

59 The ability of V. vulnificus to cause disease is linked to the producti

60 Inactivation of pilA reduces the ability of V. vulnificus to form biofilms and significantly decreas

61 ed a genome-wide transcriptional analysis of V. vulnificus growing at three different iron concentrat

62 ar typing systems have shown associations of V. vulnificus genotypes and the environmental or clinica

63 We report here the first case of V. vulnificus primary bacteremia due to raw shellfish co

64 Recent cases of V. vulnificus infections in Los Angeles County suggest t

65 port are present, suggesting that the CPS of V. vulnificus is lipid linked.

66 cutaneously with 10 times the lethal dose of V. vulnificus and injected intravenously, either simulta

67 2 X 10(9) colony-forming unit (high dose) of V. vulnificus was administered through a mini-laparotomy

68 highly similar to the putative epimerase of V. vulnificus.

69 dicate that an important virulence factor of V. vulnificus is undergoing significant genetic rearrang

70 nce potential between these two genotypes of V. vulnificus.

71 16S rRNA gene, there are two major groups of V. vulnificus designated types A and B.

72 ines the growing international importance of V. vulnificus, particularly in the context of coastal wa

73 sertion mutagenesis in a clinical isolate of V. vulnificus to find genes necessary for virulence, and

74 olved to facilitate the aquatic lifestyle of V. vulnificus but that their emergence also resulted in

75 les are present on the lipopolysaccharide of V. vulnificus, are required for full motility and biofil

76 ge of human-derived peptides by PGI-LysAP of V. vulnificus using three approaches: (i) a quantitative

77 oculated iron dextran-treated mouse model of V. vulnificus disease, was hypersensitive to the fatty a

78 us in an iron-dextran-treated mouse model of V. vulnificus infection.

79 capsulated and nonencapsulated morphotype of V. vulnificus.

80 We previously constructed a fur mutant of V. vulnificus which constitutively expresses at least tw

81 The hupA deletion mutant of V. vulnificus will be helpful in future studies of the r

82 variants and genetically defined mutants of V. vulnificus M06-24/O was examined by using a CPS-speci

83 k correlates with seasonally high numbers of V. vulnificus bacteria during the summer months.

84 ersus genetic deletions in the CPS operon of V. vulnificus.

85 ntial role of capsule in the pathogenesis of V. vulnificus.

86 hich may contribute both to pathogenicity of V. vulnificus and to its survival under adverse environm

87 pulsed-field gel electrophoresis profiles of V. vulnificus strains isolated from blood and oysters as

88 In contrast, the growth rate of V. vulnificus in alkaline peptone water was greater than

89 ructural gene for HupA, the heme receptor of V. vulnificus.

90 l gene for VuuA, the vulnibactin receptor of V. vulnificus.

91 Analysis of the promoter region of V. vulnificus hupA showed a sequence homologous to the c

92 We previously described two sets of V. vulnificus strains with different levels of virulence

93 saccharide of a related pathogenic strain of V. vulnificus (MO6-24) the structure of which was recent

94 accharide purified from a virulent strain of V. vulnificus 6353 did not show cross reactivity with an

95 enome of an encapsulated, clinical strain of V. vulnificus.

96 Biotype 1 strains of V. vulnificus are most commonly associated with human in

97 pimerase is common to at least 10 strains of V. vulnificus that each express a serologically distinct

98 lar polysaccharides of pathogenic strains of V. vulnificus, there are distinct differences in the det

99 clinical as well as environmental strains of V. vulnificus.

100 e isolates with colony morphology typical of V. vulnificus identified 75% as V. sinaloensis.

101 nked to a previously unrecognized variant of V. vulnificus designated biotype 3.

102 lluscan shellfish are the primary vectors of V. vulnificus disease.

103 asize the importance of CPS for virulence of V. vulnificus and establish a correlation between CPS ex

104 nd septicemia in humans and the virulence of V. vulnificus has been strongly associated with encapsul

105 ng that in addition to capsule, virulence of V. vulnificus requires type IV pili and/or extracellular

106 mes cannot strictly predict the virulence of V. vulnificus strains and further investigation is neede

107 se, exopolysaccharides, and the virulence of V. vulnificus.

108 e pili, suggesting that the pili observed on V. vulnificus are of the type IV class.

109 ulence properties compared to those of other V. vulnificus strains.

110 ted Vibrio species were V. parahaemolyticus, V. vulnificus, and V. alginolyticus; both surveillance s

111 r species (V. cholerae, V. parahaemolyticus, V. vulnificus, and V. mimicus).

112 us Vibrio: V. cholerae, V. parahaemolyticus, V. vulnificus, and V. mimicus.

113 We conclude that V. parahaemolyticus, V. vulnificus, V. cholerae and subpopulations that harbo

114 prevalence of total Vibrio parahaemolyticus, V. vulnificus and V. cholerae and select genes associate

115 inolysis within the zoonotic marine pathogen V. vulnificus.

116 minated raw oysters, the incidence of severe V. vulnificus disease is low.

117 ated with human infections and that a single V. vulnificus strain, evidenced by pulsed-field gel elec

118 All six V. vulnificus strains caused identical skin lesions in s

119 d that V. sinaloensis grew more rapidly than V. vulnificus in seawater at temperatures </= 30 degrees

120 These studies provide evidence that V. vulnificus CPS directly stimulates the expression and

121 Through complementation we show that V. vulnificus is capable of using different TtpC2 protei

122 The V. vulnificus pilA gene is part of an operon and is clus

123 sion analysis localized one promoter for the V. vulnificus hupA gene.

124 ence of the 77-kDa protein purified from the V. vulnificus fur mutant had 67% homology with the first

125 An internal deletion in the V. vulnificus hupA gene, done by using marker exchange,

126 An internal deletion of the V. vulnificus vuuA gene resulted in the loss of expressi

127 ablished that the MARTXVv toxin is linked to V. vulnificus dependent induction of apoptosis, but the

128 tood; however, its phenotypic resemblance to V. vulnificus and the possibility that it could outcompe

129 ecrosis factor alpha elicited in response to V. vulnificus and measured in cell supernatants were not

130 ess proinflammatory cytokines in response to V. vulnificus.

131 lear cell inflammatory cytokine responses to V. vulnificus.

132 tive stress have increased susceptibility to V. vulnificus septicemia.

133 The prevalence of total V. vulnificus and the siderophore-related viuB gene also

134 t common rtxA1 gene variant in clinical-type V. vulnificus encodes a toxin with reduced potency and i

135 lated from blood and oysters associated with V. vulnificus disease.