ライフサイエンスコーパス: WGA

1 WGA allows amplification of the entire genome, which gre

2 WGA bound exclusively to SA residues on the apical surfa

3 WGA cells represent a powerful system to study the regul

4 WGA samples have high call rates (97.5% on average, comp

5 WGA was demonstrated to have high specificity for Lp(a)

6 WGA, MAA, and concanavalin A significantly inhibited fer

7 WGA-Fc directly inhibited fungal growth in standard cult

8 WGA-Fc opsonization increased fungal phagocytosis, as we

9 WGA-HRP injections in conjunction with GAD immunohistoch

10 WGA-X enhances genome recovery from individual microbial

11 Following this strategy, 20 WGA products from six Cryptosporidium species or genotyp

12 wever, showed significant contamination in 5 WGA products (proportion of positive colonies derived fr

13 Fifty nanoliters of 2.5% WGA-HRP were microinjected into the NMC in the cat.

14 nterpret the set of P-values emerging from a WGA study.

15 label in these same regions as a result of a WGA-HRP injection suggests that the connections are reci

16 involves binding of synthesized chitin to a WGA-coated surface followed by detection of the polymer

17 ear the base of the heart of muskrats with a WGA-HRP solution to label retrogradely preganglionic par

18 In addition, WGA-HRP labeling was occasionally observed in lamina I.

19 ing sites is ca. 9 A, wheat germ agglutinin (WGA) (shortest distance between binding sites 13-14 A),

20 ther, coexpression of wheat germ agglutinin (WGA) and an axon-targeted beta-gal supports mapping both

21 ciated virus encoding wheat germ agglutinin (WGA) and by immunoelectron microscopy determined the pre

22 ere we use the lectin wheat germ agglutinin (WGA) as ligand; WGA inhibits nuclear transport and has b

23 Wheat germ agglutinin (WGA) binds to the glycosylated extracellular domain III

24 Wheat germ agglutinin (WGA) binds with high affinity and specificity to several

25 Wheat germ agglutinin (WGA) bound only to the outermost region of J3 and produc

26 l PM by absorption on wheat germ agglutinin (WGA) did not deplete G alpha subunits.

27 ble to HRP-conjugated wheat germ agglutinin (WGA) disrupted delivery of HA but not endolyn.

28 st walls (PCWs) or to wheat germ agglutinin (WGA) inhibited excystation by > 90%.

29 romatography and by a wheat germ agglutinin (WGA) lectin affinity column.

30 Wheat germ agglutinin (WGA) reactive glycans on fibronectin and thrombospondin-

31 Wheat germ agglutinin (WGA) showed significantly decreased binding to adenomas

32 neuronal tract tracer wheat germ agglutinin (WGA) to nonpeptidergic nociceptive neurons.

33 anavalin A (ConA) and wheat germ agglutinin (WGA) to their target monosaccharides indicating affinity

34 gent against mycosis, wheat germ agglutinin (WGA) was linked to the effector Fc region of murine IgG2

35 transneuronal tracer, wheat germ agglutinin (WGA), in the 5HT neurons so as to study the interplay be

36 transneuronal tracer, wheat germ agglutinin (WGA), is induced in primary sensory neurons, but only af

37 ed-conjugated lectin, wheat germ agglutinin (WGA), which binds SA residues.

38 by Co2+, followed by wheat germ agglutinin (WGA)-affinity HPLC or size-exclusion HPLC (SE-HPLC) usin

39 The retrograde tracer wheat germ agglutinin (WGA)-apoHRP-gold was used to identify neurons with appro

40 eveloped by employing wheat germ agglutinin (WGA)-coated Flashplates.

41 By using wheat germ agglutinin (WGA)-coated scintillation proximity assay (SPA) beads to

42 ydrate affinity using wheat germ agglutinin (WGA).

43 blocked by the lectin wheat germ agglutinin (WGA).

44 ession of the lectin, wheat germ agglutinin (WGA).

45 cell-binding ligand, wheat germ agglutinin (WGA).

46 ivity with the lectin wheat germ agglutinin (WGA).

47 ), jacalin (JAC), and wheat germ agglutinin (WGA).

48 avalin A (Con A), and wheat germ agglutinin (WGA).

49 oxidase conjugated to wheat germ agglutinin (WGA-HRP) with biotinylated dextran amine (BDA) transport

50 The lectin, wheatgerm agglutinin (WGA) conjugated to horseradish peroxidase (HRP), previou

51 e general problem of whole-genome alignment (WGA).

52 was processed by whole genome amplification (WGA) and hybridized onto a D. mccartyi microarray for co

53 chnologies using whole genome amplification (WGA) and preimplantation genetic haplotyping (PGH) of em

54 rcalator dye and whole genome amplification (WGA) assays.

55 ping methods for whole genome amplification (WGA) by random or degenerate oligonucleotide-primed PCR.

56 me this is to do whole-genome amplification (WGA) in clinical samples, but only limited studies have

57 Whole-genome amplification (WGA) is a useful tool for amplification of very small qu

58 f the GenomePlex whole genome amplification (WGA) kit to amplify frozen and FFPE tissue for use in ar

59 ts introduced in whole-genome amplification (WGA) make it difficult to derive accurate genomic inform

60 However, current whole-genome amplification (WGA) methods are limited by low accuracy of copy-number

61 Whole-genome amplification (WGA) methods offer a solution for this problem, and earl

62 cation steps and whole-genome amplification (WGA) of DNA from purified oocysts.

63 NA obtained from whole genome amplification (WGA) of RNA and DNA from sewage and animal (avian, cattl

64 Whole genome amplification (WGA) of single cells is generally the first step in such

65 on sequencing, a whole-genome amplification (WGA) process is required, but it substantially distorts

66 ection (LCM) and whole-genome amplification (WGA) to separately collect and amplify DNA from adjacent

67 ally single cell whole genome amplification (WGA) was employed to pre-amplify the single-cell DNA by

68 ein analysis and whole genome amplification (WGA), and we demonstrate this by doing both for the same

69 cell sorting and whole-genome amplification (WGA), we are able to generate high-quality material from

70 cation (MDA) for whole-genome amplification (WGA), which is followed by high-throughput sequencing.

71 have developed a whole genome amplification (WGA)-based genotyping assay for PKD1 and PKD2, and exami

72 procedure before whole genome amplification (WGA).

73 specific PCR and whole genome amplification (WGA).

74 DNA content and whole-genome amplification (WGA); this is followed by next-generation sequencing to

75 Weak interactions with MAL, Con A, and WGA were also quantified.

76 Subsequent application of the FACS and WGA protocols to two enrichment cultures containing appr

77 A significantly inhibited fertilization and WGA was found to block fertilization by preventing sperm

78 LM-PCR and WGA, the most popular sample amplification techniques, r

79 Exposure of cysts to anti-PCW and WGA prior to, but not after, stage II was sufficient to

80 ar antigen, which is composed of protein and WGA reactive carbohydrate, and indicate that cross-react

81 from the Q-Sepharose, heparin-Sepharose, and WGA-agarose also indicated only the single radiolabeled

82 raphy on Q-Sepharose, heparin-Sepharose, and WGA-agarose.

83 We have further applied WGA to ADPKD mutation analysis of low DNA-yield specimen

84 framework of post-whole genome association (WGA) annotation, we have developed WGAViewer, a suite of

85 were identified by whole genome association (WGA) mapping of cytokine concentrations.

86 MDA-based WGA is a simple and reliable method that could have sign

87 ast to 4-6 orders of magnitude for PCR-based WGA methods.

88 trograde labeling of trigeminal afferents by WGA injection into the tip of the tongue showed an incre

89 ated by anti-Hs immunoglobulin G and also by WGA.

90 Inhibition by WGA was reversed by either chitotriose or sialic acid, w

91 8 weeks, ocular dominance stripes labeled by WGA-HRP appeared adultlike with smooth, sharply defined

92 The area of cell bodies labeled by WGA-HRP appeared similar to the area of cell bodies labe

93 Here we report an improved single-cell WGA method, Linear Amplification via Transposon Insertio

94 wn to exceed previously reported single-cell WGA methods in amplification uniformity, genome coverage

95 t performance in high-throughput single-cell WGA remains elusive.

96 ected for further investigation by combining WGA analysis with previously published QTL for murine re

97 In conclusion, WGA-based LR-PCR represents a major technical improvemen

98 supporting nor globose basal cells contained WGA-HRP, suggesting that uptake was primarily into olfac

99 bution of NRA axons in the lumbosacral cord, WGA-HRP injections were made into the NRA in seven monke

100 ng phased parental genotypes and deciphering WGA-distorted SNP B-allele fractions via a process we co

101 lied for the first time a recently developed WGA method, multiple displacement amplification (MDA), t

102 t only limited studies have tested different WGA methods in FFPE cancer specimens using targeted next

103 These findings suggest that each WGA method should be tested thoroughly before using it f

104 Although numerous studies employing WGA have focused primarily on clinical applications, few

105 However, existing WGA methods like degenerate oligonucleotide-primed PCR s

106 As expected, WGA products with low (<16.0) threshold cycle (CT) value

107 Both approaches resulted in extensive WGA expression in the cell bodies and dendrites of neuro

108 The evolving technology of PGH following WGA may increase the diagnostic scope and availability o

109 ed nucleus of the stria terminalis following WGA-Au-HRP injections that incorporated the rostrolatera

110 Triple labeling for WGA-Au, Fos, and orexin revealed that the percentage of

111 The tissue was processed sequentially for WGA-HRP, and then BDA immunohistochemistry using two dif

112 er aberrations have to be discriminated from WGA artifacts.

113 ding on the application, background DNA from WGA kits can be problematic.

114 d to horseradish peroxidase coupled to gold (WGA-Au-HRP) was injected into either the nucleus LC or t

115 al cord involved placement of the tracer HRP/WGA-HRP on the cut end of the nerve root.

116 Changes in WGA binding to the human surface epithelium allowed regi

117 pre-amplification is the only method used in WGA of single sperm, whereas it has limited capacity to

118 , some inter- and intrasample variability in WGA was observed, but these biases were minimized by per

119 bohydrates and a range of lectins, including WGA, Con A, UEA-I, GS-II, PNA and SBA, were monitored in

120 By integrating WGA-X with calibrated index-cell sorting and high-throug

121 peptide sequence was obtained from a 220-kDa WGA-binding protein.

122 It behaves as a 250 kDa, WGA-reactive glycoprotein with a basic isoelectric point

123 Double-labeled WGA-HRP/ChAT neurons were found in the pedunculopontine

124 Double-labeled WGA-HRP/NADPH-d-positive neurons could be seen in many n

125 llustrate the diverse uses of these ZW (LacZ-WGA) mice, we triggered WGA expression either by crossin

126 We show evidence that LCM, WGA, and NGS of adjacent tumor regions provide an import

127 is inhibited by microinjection of a lectin, WGA, without affecting the normal inactivation of the M-

128 ectin wheat germ agglutinin (WGA) as ligand; WGA inhibits nuclear transport and has been shown to dir

129 vel differentiated human hepatoma cell line (WGA), derived from HepG2, which expresses CYP2B6 and CAR

130 l evaluation of existing methods, a modified WGA strategy was developed that appears to have utility

131 rily resistant to the effects of neurotoxin, WGA-LH(N)/A conjugate potently inhibits secretory respon

132 ttern of PAG axons in the medulla oblongata, WGA-HRP was injected into the PAG and adjacent tegmentum

133 Hence, the opsonic activity of WGA-Fc effectively modulates fungal cell recognition and

134 Administration of WGA-Fc also dramatically diminished pulmonary inflammati

135 Prophylactic administration of WGA-Fc fully protected mice against H. capsulatum, corre

136 Immunoblot analysis of WGA bound membrane proteins crosslinked with DSS identif

137 Quantitative PCR (qPCR) analysis of WGA products was used as an initial quality assessment o

138 The decreased binding of WGA and HPA to the luminal surface epithelium in human d

139 thodology was tested by measuring binding of WGA to the surface of confluent monolayers of living Cac

140 of a three-part nanoconjugate consisting of WGA-HRP, AuNPs, and drugs for the treatment of diaphragm

141 acts, primarily on the proximal dendrites of WGA-HRP-labeled motoneurons.

142 A heavy density of WGA-HRP-labeled neurons was found in the ipsilateral mes

143 Finally, we found very few examples of WGA-immunoreactive noradrenergic neurons, which suggests

144 Following injections of WGA-Au-HRP into the nuclear LC, triple labeled neurons w

145 amber and anteromedian nucleus injections of WGA-HRP in the same animal.

146 Injections of WGA-HRP into OPt labeled terminals bilaterally in the an

147 Injections of WGA-HRP into the anteromedian nucleus labeled fusiform p

148 Injections of WGA-HRP into the ventral (MGv), dorsal (MGd), or medial

149 Single injections of WGA-HRP or discrete injections of red and green latex mi

150 Large intraocular injections of WGA-HRP were placed into the eye, and patterns of labele

151 roup of animals was given microinjections of WGA-HRP in the medial nucleus of the medial geniculate (

152 Complementary interdigitating patches of WGA-HRP and BDA labeling were found primarily in transit

153 To determine the performance of WGA products on a large-scale genotyping array, we compa

154 A 7- to 10-fold higher proportion of WGA- and anti-PCW-treated cysts than control cysts remai

155 The purity of WGA products was assessed by Sanger sequencing of cloned

156 the phenomenon of transsynaptic transfer of WGA-HRP after injection into the olfactory bulb or rats

157 ury there was no intraganglionic transfer of WGA.

158 ously accessing genomic information based on WGA.

159 nsity was assessed by di-4-ANEPPS, FM4-64 or WGA staining using confocal microscopy.

160 s detected in the vast majority of PHA-L- or WGA-positive terminals forming asymmetric synapses.

161 We found that PHA-L- or WGA-positive terminals from tagged VTA cells made asymme

162 termined the presence of VGluT2 in PHA-L- or WGA-positive terminals.

163 radish peroxidase coupled to gold particles (WGA-Au-HRP) or fluorescein-conjugated latex beads, into

164 tify the neurons of origin for this pathway, WGA-HRP injections were centered in the CCS.

165 Two percent WGA-HRP was injected into the lower thoracic/upper lumba

166 utinin conjugated to horseradish peroxidase (WGA-HRP) and fluorescent dyes were made into the medial

167 heat germ agglutinin-horseradish peroxidase (WGA-HRP) and up to four different fluorochromes in V2 la

168 utinin conjugated to horseradish peroxidase (WGA-HRP) and up to four fluorochromes.

169 heat germ agglutinin-horseradish peroxidase (WGA-HRP) as a tracer.

170 germ agglutinin and horseradish peroxidase (WGA-HRP) combined with glutamate and choline acetyltrans

171 heat germ agglutinin-horseradish peroxidase (WGA-HRP) from olfactory sensory neurons to the olfactory

172 heat germ agglutinin-horseradish peroxidase (WGA-HRP) from the ventral hippocampal formation or by an

173 heat germ agglutinin-horseradish peroxidase (WGA-HRP) in dorsal (PMD) and ventral (PMV) premotor area

174 heat germ agglutinin-horseradish peroxidase (WGA-HRP) in the left nodose ganglion.

175 gglutinin-conjugated horseradish peroxidase (WGA-HRP) injections revealed differences in the pattern

176 wheatgerm agglutinin-horseradish peroxidase (WGA-HRP) into ICXv.

177 gglutinin-conjugated horseradish peroxidase (WGA-HRP) into the area of the CCS revealed a distinctive

178 gglutinin-conjugated horseradish peroxidase (WGA-HRP) into the beta-nucleus and dmcc of the inferior

179 gglutinin-conjugated horseradish peroxidase (WGA-HRP) into the muscle.

180 utinin conjugated to horseradish peroxidase (WGA-HRP) into the SC, the heaviest concentrations of lab

181 heat germ agglutinin-horseradish peroxidase (WGA-HRP) or choleragenoid-horseradish peroxidase (B-HRP)

182 heat germ agglutinin-horseradish peroxidase (WGA-HRP) or cytochrome oxidase (CO) histochemistry, afte

183 heat germ agglutinin-horseradish peroxidase (WGA-HRP) placed into the PM resulted in widespread anter

184 heat germ agglutinin-horseradish peroxidase (WGA-HRP) was injected into the lumbosacral cord in three

185 heat germ agglutinin horseradish peroxidase (WGA-HRP) was injected into the NRA in six monkeys.

186 inin conjugated with horseradish peroxidase (WGA-HRP) were made into dorsal/ventral striatum (DS/VS),

187 utinin conjugated to horseradish peroxidase (WGA-HRP) were made into the ferret's superior colliculus

188 utinin conjugated to horseradish peroxidase (WGA-HRP) were placed in caudal (CPB) and rostral (RPB) d

189 inin conjugated with horseradish peroxidase (WGA-HRP) were placed in topographically different locati

190 wheatgerm agglutinin-horseradish peroxidase (WGA-HRP), BDA, or a fluorescent tracer, iontophoreticall

191 heat-germ agglutinin-horseradish peroxidase (WGA-HRP), the carbocyanine dye DiI, and biocytin) to det

192 heat germ agglutinin-horseradish peroxidase (WGA-HRP)-labeled spinocerebellar mossy fiber terminals i

193 utinin conjugated to horseradish peroxidase (WGA-HRP).

194 eat germ agglutinin horse radish peroxidase (WGA-HRP) chemically conjugated to gold nanoparticles (Au

195 of the polymer with a horseradish peroxidase-WGA conjugate.

196 that a nonadherent yolk sac cell population (WGA+, density < 1.077, AA4.1+) can give rise to B cells,

197 Here we present WGA-X, a method based on multiple displacement amplifica

198 ned with a library preparation without prior WGA using an NEBNext Ultra II paired-end kit, which requ

199 Therefore, we propose WGA-Fc as a potential "pan-fungal" therapeutic that shou

200 s a slight decrease in the total call rates, WGA methods provide a reliable approach for increasing t

201 The resultant WGA-Fc displayed high affinity to purified chitin and bo

202 led with succinyl Triticum vulgare lectin (S-WGA) and represents the major DBA-binding component in T

203 tated with C-CAM from detergent solubilized, WGA-purified proteins.

204 is by a whole genome amplification strategy (WGA).

205 re and its contributive role in a successful WGA project.

206 Succinylated WGA reveals differences in these axon classes earlier, a

207 on SPA beads, were even more effective than WGA-coated SPA beads for capturing the insect cells.

208 t IB4-HRP was a much less robust tracer than WGA-HRP.

209 These findings highlight the impact that WGA kit selection can have on metagenomic analysis of lo

210 Our results indicate that WGA product performs well on the 250K array compared to

211 The percentages that WGA-HRP retrogradely labelled neurons composed of the pr

212 problem, and earlier studies have shown that WGA samples perform reasonably well in small-scale genet

213 l, and then microarray results verified that WGA from 10(6) cells or approximately 1 ng of genomic DN

214 The WGA-Flashplate is suitable for fully automated high-thro

215 n label the circuits that are engaged by the WGA-expressing damaged neurons.

216 However, we also found the WGA tracer in DRG cell bodies of uninjured sensory neuro

217 n the fluorescence assay but negative in the WGA assay.

218 ches that correct for biases inherent in the WGA procedure and allow for accurate determination of co

219 Mutant BRAF in the WGA-amplified genomic DNA was further amplified by a two

220 Compared to the gDNA method the WGA-based assay had a sensitivity and specificity of 100

221 negative in the fluorescence assay while the WGA results for these two kits were ambiguous.

222 genotyping results of genomic DNA and their WGA products from four individuals.

223 This WGA method should readily lend itself to the determinati

224 Three WGA kits were tested for their utility in a metagenomics

225 carboxamide riboside, an AMPK activator, to WGA and human hepatocytes induces CYP2B6 gene expression

226 onditions, three additional proteins bind to WGA-Sepharose and are revealed by the organelle trap ass

227 which were primarily found contralateral to WGA-HRP muscle injections.

228 188-Nup205 complex does not bind directly to WGA but binds indirectly via the N-acetylglucosamine-mod

229 nilateral injections of a retrograde tracer (WGA-Au, 350-400 nl) were made into the VTA or a nonrewar

230 inase promotes the expression of the tracer, WGA (wheat germ agglutinin), and used these in combinati

231 We used the transganglionic tracers WGA-HRP, IB4-HRP, and CTB-HRP to trace the central proje

232 nd histochemical localization of transported WGA-HRP or B-HRP was performed.

233 xpected, sciatic nerve transection triggered WGA expression in NPY-positive DRG neurons, most of whic

234 es of these ZW (LacZ-WGA) mice, we triggered WGA expression either by crossing the mice with two Cre-

235 therefore tested the two most commonly used WGA methods, multiple displacement amplification (MDA-Qi

236 sialylated from hyposialylated glycans, used WGA, SNA, PNA, Jacalin, HPA, and VVA, indicating glomeru

237 m retrograde tracing were confirmed by using WGA-HRP as an anterograde tracer from input sources.

238 ntocellularis and magnocellularis, that were WGA-immunoreactive, i.e., were transneuronally labeled f

239 s study demonstrates that coupling FACS with WGA and microarrays is a promising tool to expedite geno

240 noperated group (controls) was injected with WGA-HRP in the left nodose ganglion.

241 weeks, following intranasal irrigation with WGA-HRP.

242 f AEN trigeminal ganglion cells labeled with WGA-HRP, and (2) electron microscopic analysis of the AE

243 did not react with anti-Hs antibody or with WGA.

244 body reactivity and restores reactivity with WGA.