ライフサイエンスコーパス: Western analysis

1 s LNCaP, PC-3, and DU-145 using quantitative Western analysis.

2 ERbeta protein expression was determined by Western analysis.

3 evel using ribonuclease protection assay and western analysis.

4 as specifically reduced their expression, by Western analysis.

5 abrogated PMS2 protein in germline cells by Western analysis.

6 nd LV tissue levels of CIMP were measured by Western analysis.

7 spectrometry observations were confirmed by Western analysis.

8 jected to proteomic analysis and verified by Western analysis.

9 l PKCbeta protein expression was assessed by Western analysis.

10 ger (NCX), and phospholamban was assessed by Western analysis.

11 erexpression of OGT protein as determined by Western analysis.

12 ature peptide intracellularly as detected by Western analysis.

13 rease in FliC protein levels was observed by Western analysis.

14 ein (MAP) kinase phosphorylation measured by Western analysis.

15 ion was confirmed by both DNA sequencing and Western analysis.

16 ic epithelial cell coculture supernatants by Western analysis.

17 transcriptase-polymerase chain reaction and Western analysis.

18 NGF receptor activation was assessed by western analysis.

19 the presence of p53 and pRb was detected by Western analysis.

20 expressed abundant levels of LRAT protein by Western analysis.

21 2 protein in these cells was demonstrated by Western analysis.

22 sults were confirmed at the protein level by Western analysis.

23 se in nuclear levels of p53 as determined by Western analysis.

24 -transcriptase polymerase chain reaction and Western analysis.

25 y 60-kDa membrane protein (RFC) was shown by Western analysis.

26 ibular salivary gland cells using RT-PCR and Western analysis.

27 und in rat submandibular gland by RT-PCR and Western analysis.

28 cyltransferase) was assessed by Northern and Western analysis.

29 in promoter binding sequence as probe and by Western analysis.

30 and sigmaG protein levels were determined by Western analysis.

31 -binding motif was shown to bind PCNA by far-Western analysis.

32 in vivo are 0.5-2.3 microM, as determined by Western analysis.

33 pared with PBMCs using coimmunoprecipitation/Western analysis.

34 xes were identified by mass spectrometry and Western analysis.

35 red with PBMCs was confirmed by quantitative Western analysis.

36 retinas examined by confocal microscopy and western analysis.

37 essed in soluble form and recognized Ku86 by Western analysis.

38 INY), during growth in vitro, as assessed by Western analysis.

39 assays, NFI proteins are still detectable by Western analysis.

40 d the kinetics of induction were measured by western analysis.

41 ite detection of endogenous TRAF2 and NIK by Western analysis.

42 rotein or denatured purified APV proteins by Western analysis.

43 extracted, and immunoprecipitated, prior to Western analysis.

44 ptase polymerase chain reaction (RT-PCR) and Western analysis.

45 ontrol brains using immunohistochemistry and Western analysis.

46 expressed in mice as assayed by Northern and Western analysis.

47 ultiple splice forms as evidenced by PCR and Western analysis.

48 -tagged IKLF in transfection experiments and western analysis.

49 esorbing osteoclasts as shown by qRT-PCR and Western analysis.

50 vitro was determined by EPR spectroscopy and Western analysis.

51 istinct isoforms by two-dimensional SDS-PAGE Western analysis.

52 in opa1 protein in retina and all tissues on western analysis.

53 reason for its earlier lack of detection by western analysis.

54 expression of CDK1 was further validated by Western analysis.

55 both infected COS-7 cells and avian cells by Western analysis.

56 ablish further a FIH-1/c-kit interaction via Western analysis.

57 n (1.6:1, P=0.07) and fibronectin synthesis (Western analysis, 1.3:1, P<0.01; chromatography, 1.9:1,

58 ium, as determined by both RT-PCR (9/10) and Western analysis (6/8).

59 Western analysis also confirmed attenuated expression of

60 DAX-1 and SF-1 mRNA in whole human skin and Western analysis also confirmed the presence of DAX-1 pr

61 Western analysis also indicated that stimulated mice exh

62 Western analysis also revealed that exosome-like vesicle

63 Western analysis also showed that Q bonds to the E2 comp

64 Western analysis also showed that the enzyme was present

65 Using immunoprecipitation, Western analysis, amino acid sequencing and end-point di

66 to biologically active ANP, as determined by Western analysis and a cyclic GMP assay.

67 Western analysis and chemical stability studies showed t

68 with MLH1 in the yeast-two hybrid assay; far Western analysis and co-immunoprecipitations confirmed t

69 In this study, Western analysis and confocal immunocytochemistry were u

70 on of MRGX with HDAC1 by immunoprecipitation/Western analysis and determined that MRGX complexes had

71 Western analysis and electrophoretic mobility shift assa

72 p53 was undetectable by Western analysis and examination of the proapoptotic pro

73 nonuclear cells (PBMCs) was examined by PCR, western analysis and flow cytometry, with amniocytes and

74 ble at the protein level, as demonstrated by western analysis and flow cytometry.

75 model for this disease were characterized by Western analysis and found to express all three PPAR iso

76 The results of Western analysis and gel retardation experiments suggest

77 d MUC2 protein expression were determined by Western analysis and immunocytochemistry.

78 wild-type and PDZK1-null mutant mice by both Western analysis and immunocytochemistry.

79 However, western analysis and immunofluorescence microscopy of ma

80 Western analysis and immunofluorescence staining for cyc

81 Using Western analysis and immunogold labeling, A-RAF was sele

82 gs from humans and baboons were evaluated by Western analysis and immunohistochemistry.

83 Western analysis and immunostaining of COS-7 transfectan

84 ve hCGBP is detected as an 88-kDa protein by Western analysis and is ubiquitously expressed.

85 Western analysis and light level immunocytochemistry usi

86 t of the heterodimer, Ku70, as determined by Western analysis and mass spectrometry.

87 n all three reading frames were confirmed by western analysis and mass spectrometry.

88 We have used Far Western analysis and phosphopeptide competition assays t

89 HP adducts were also characterized by Western analysis and quantified by competitive enzyme-li

90 are reduced in the hippocampus, measured by Western analysis and radioligand binding assay, although

91 signal-regulated kinase (ERK) activation by Western analysis and Src in vitro kinase assay.

92 quence (MRPFL) was shown to bind PCNA by far Western analysis and to compete with p50 for binding to

93 vitro to isolated denatured keratins in Far-Western analysis and to native IFs in pull-down assays.

94 nd seed maturation mutants of Arabidopsis by western analysis and using an HSP17.4 promoter-driven be

95 Immunological detection based on immunoblot (Western) analysis and immunogold labeling correlated pos

96 t HUPF1 cross-reacted with delta helicase on western analysis, and delta helicase activity was immuno

97 and PGE(2) levels were quantified by RT-PCR, Western analysis, and enzyme immunoassay, respectively.

98 MMP secretion was measured by zymography and Western analysis, and expression in patients with pulmon

99 -PCR, for protein by immunocytochemistry and Western analysis, and for their potential to catalyze NA

100 and melanoma cell lines by RNase protection, Western analysis, and immunohistochemistry.

101 rats and assessed by RNA protection assays, Western analysis, and tissue immunofluorescence.

102 COS-7 and HeLa cells express ArgBP2 (by Western analysis), but expression was detectable only in

103 Western analysis confirmed that AdOprF.RGD.Epi8 expresse

104 Western analysis confirmed that alpha-CA was bound to th

105 Quantitative reverse transcription-PCR and Western analysis confirmed that GLUT4 levels are greatly

106 Western analysis confirmed that the phosphorylation of k

107 Northern and Western analysis confirmed the absence of uroguanylin me

108 in 1 was expressed in secretory cells, while Western analysis confirmed the decreased expression of i

109 Western analysis confirmed the down-regulation of HMG-I(

110 Western analysis confirmed the induction of BBC3 protein

111 Western analysis confirmed the presence of V. cholerae f

112 Quantitative real-time PCR and Western analysis demonstrate that SPRM1hc is expressed i

113 ls revealed normal amounts of MafA mRNA, but Western analysis demonstrated a 97 +/- 1% reduction in M

114 Far Western analysis demonstrated a direct association betwe

115 172 antibody (binds only to activated AMPK), Western analysis demonstrated active AMPK in both FSH- o

116 Immunohistochemistry and western analysis demonstrated downregulation of p63 in N

117 Western analysis demonstrated increased phosphorylation

118 Immunostaining and Western analysis demonstrated that the p85 isoform of PI

119 Western analysis demonstrated that the translocation-com

120 Western analysis demonstrated the near absence of both i

121 transcriptase-polymerase chain reaction and Western analysis demonstrated the presence of the acLDL

122 Western analysis demonstrates that a compensatory feedba

123 Western analysis detected a 53% increase in the Na(+)-Ca

124 For both recombinant and native proteins, western analysis detected MERTK interactions with GRB2,

125 Western analysis did not reveal any significant changes

126 Western analysis for (ir)GCS showed a significant decrea

127 UTP nick-end labeling assay, and ex vivo via Western analysis for active caspase-3.

128 ese data, which were validated by qRT-PCR or Western analysis for ID1, TP53, HPSE, NQO1, EGR1, and VE

129 dogs, AGE content (immunohistochemistry and Western analysis for N(epsilon)-(carboxymethyl)lysine [C

130 a (amy) calbindin and calretinin levels (via Western analysis) from animals fed a phytoestrogen-free

131 Western analysis further showed that NGF increased nucle

132 Western analysis has confirmed corresponding increases i

133 Ribonuclease protection and Western analysis has shown that hypoxia induces EndoPDI

134 In Western analysis, HAT-L4 expressed in transfected CHO ce

135 somatic cells of mouse and rat testes using Western analysis, high-resolution single-cell bioimaging

136 trophoretic mobility shift assays coupled to Western analysis identified NF-E2-related proteins 1 and

137 uction in kinase expression was confirmed by Western analysis, immunocytochemistry, and exogenous sub

138 ess protein levels and localization, we used Western analysis, immunohistochemistry, and immunofluore

139 ured HBD2 concentrations by semiquantitative Western analysis in BAL of prelung transplant patients (

140 Within 2h, recombinant OGT was detected by Western analysis in both cytosol and nucleosol.

141 was observed by both immunocytochemistry and Western analysis in cultures challenged with BaP compare

142 S, eNOS, CM, sGC, NAD(P)H oxidase and SOD by Western analysis in different regions of the normal rat

143 The protein levels of CILP/NTPPH by Western analysis in the media from adult and young porci

144 wed decreased phosphorylation at Ser(473) by Western analysis in the presence of PI 3-kinase inhibito

145 Western analysis indicated corresponding increases in FP

146 Western analysis indicated degradation of 68 kDa neurofi

147 Western analysis indicated that mutations in all three l

148 uorescence activated cell sorting (FACS) and western analysis indicated that this missense mutation c

149 Reverse transcription-PCR and Western analysis indicated the down-regulation of the RO

150 Reverse transcription-PCR and Western analysis indicated the expression of RON message

151 sion was predominant in the cytoplasm, which western analysis indicated was due to the cleavage of fi

152 Western analysis indicates that the CdrA is produced as

153 was converted to mature ANP as determined by Western analysis, indicating the presence of the endogen

154 MAb-174 also performed very well in Western analysis, indirect fluorescence microscopy, and

155 inary excretion, as measured by quantitative Western analysis, is a sensitive biologic marker to asse

156 o enhanced accuracy relative to conventional Western analysis, it circumvents the distortions inheren

157 Western analysis localized mdr1 to the apical membrane o

158 transcription- polymerase chain reaction and Western analysis normalized with purified proteins.

159 Western analysis of AdalphaV-infected cell supernatants

160 Western analysis of cell extracts localized FhuD to the

161 mmuno-electron microscopy of leaf tissue and western analysis of chloroplast fractions with monoclona

162 Western analysis of coimmunoprecipitated NS5A complexes

163 esent in the metalloprotease domain of MDC9, Western analysis of concanavalin A-enriched glomerular m

164 tosis quantification, caspase profiling, and Western analysis of cytoplasmic cytochrome c release.

165 Western analysis of endogenous MAGI-1 from glomerular pr

166 Western analysis of eNOS immunoprecipitates was used to

167 Western analysis of ERalpha suggested that the main prot

168 the mature form of ADAMTS-4 was detected by Western analysis of extracts of aortic intima.

169 However, confocal immunocytochemistry and western analysis of F98 glioma cells raise the possibili

170 In contrast, Western analysis of HDGF demonstrated increased expressi

171 Western analysis of HepG2 cell lysate using an antibody

172 Western analysis of human kidney membrane protein showed

173 s was shown by fluorescent microscopy and by Western analysis of isolated cellular fractions.

174 mmunofluorescence of intact cells as well as Western analysis of isolated lipid droplets revealed tha

175 Northern analysis of total kidney RNA or Western analysis of kidney protein homogenates from embr

176 reatment was observed by immunoprecipitation/Western analysis of lysates from p47phox(-/-) VSMCs tran

177 Western analysis of murine tissues showed that the relat

178 Far Western analysis of N- and C-terminal deletion mutants l

179 Western analysis of nasal washings demonstrated that RV

180 EMSAs and Western analysis of normal B cells before and after stim

181 ne as determined by colony formation assays, Western analysis of one-dimensional and two-dimensional

182 Western analysis of p53 was supplemented with its cytolo

183 Western analysis of PELP1/MNAR in normal and serous ovar

184 ctrophoretic mobility-shift assay (EMSA) and Western analysis of proteins bound to biotinated EMSA pr

185 Western analysis of proteins bound to pUC18 and MAR plas

186 In a western analysis of proteins from cultured human and mou

187 ially translocated F protein was detected by Western analysis of proteins in total-cell extracts of N

188 Second, Western analysis of purified beta- and non-beta-cell mem

189 Western analysis of rat glioma C6 cells or mouse bone ma

190 Western analysis of recombinant MyoXVIIIA domains expres

191 m identified 70- and 72-kDa protein bands in Western analysis of schistosome extracts.

192 The gradation of overload was confirmed by Western analysis of SERCA and CPT expression (1.6- to 7.

193 Northern and Western analysis of SPAF expression in testes of mice at

194 Western analysis of the cell extracts using phosphospeci

195 Western analysis of the conditioned medium demonstrated

196 Western analysis of the Na pump from mature human erythr

197 Western analysis of the proteins in the nuclear extracts

198 Western analysis of the receptors under nonreducing cond

199 Western analysis of these cultured progenitor cells conf

200 Western analysis of total homogenates and mixed plasma a

201 Western analysis of tuber homogenates and computer-assis

202 Western analysis of two-dimensional gels identified mult

203 Western analysis of various Late Pollen actin RNA interf

204 Western analysis of whole cell lysates showed significan

205 emistry), mRNA (RT-PCR), and protein levels (Western analysis) of AQP4 in brains from Sprague Dawley

206 erexpression of BMP4 was first detectable by Western analysis on embryonic day 16 and persisted into

207 orylated by RSK and used as ligands in a far Western analysis; only those containing Ser(P)(703) exhi

208 Both mAbs recognized rMBL-A by Western analysis or surface plasmon resonance.

209 Antibody specificity was assessed by using Western analysis, preadsorption of the antibody with pep

210 were determined in rat liver and kidney with Western analysis, real-time reverse transcriptase-mediat

211 believed to generate many splice forms, our western analysis resolved fibrocystin as a 500 kD produc

212 ein expression were analyzed by Northern and Western analysis, respectively, to determine the level o

213 t the RNA and protein level using RT-PCR and western analysis, respectively.

214 of endogenous beta-catenin, as confirmed by Western analysis, resulted in the failure to gastrulate,

215 One- and two-dimensional Western analysis reveal a complex pattern of Prospero is

216 Western analysis revealed a protein of the predicted siz

217 chieved by staurosporine (STS) exposure, and Western analysis revealed a reduction in full-length Sta

218 Immunohistochemical staining and Western analysis revealed an increase in CPD protein, an

219 ed overexpression of tylR (but not tylS) and Western analysis revealed significantly reduced levels o

220 Western analysis revealed that after induction of apopto

221 Western analysis revealed that ComW is indeed a transien

222 Western analysis revealed that conjugated ubiquitin, ind

223 Western analysis revealed that in human testes, the TSP5

224 However, Western analysis revealed that LEC12 has approximately 2

225 Western analysis revealed that levels of the full-length

226 Far Western analysis revealed that PELP1 interacts with hist

227 Western analysis revealed that PKCalpha, gamma, epsilon,

228 Western analysis revealed that TGFbeta signalling via Sm

229 Western analysis revealed that the hyperglycemia-induced

230 Western analysis revealed that the level of PML protein

231 Western analysis revealed that the major AQP4 band at ap

232 Far Western analysis revealed that ZO-2 can directly bind to

233 Immunocytochemical studies and Western analysis revealed the presence of adenosine A(1)

234 Western analysis reveals that the protein is downregulat

235 Western analysis showed a high protein level of ABCA7 in

236 Further microarray and Western analysis showed decreased expression of calcium-

237 Western analysis showed detectable hCG2 protein in norma

238 Western analysis showed enhanced expression of both prot

239 Western analysis showed SLS-mediated induction of early

240 Western analysis showed that all three strains exhibit l

241 Western analysis showed that ComS accumulates to maximal

242 Northern and Western analysis showed that Evi27 is expressed in selec

243 Western analysis showed that expression of NEP and prote

244 Western analysis showed that RSV caused no change in the

245 Quantitative real-time PCR and Western analysis showed that the mRNA and protein expres

246 Northern and Western analysis showed the presence of EBP50 messenger

247 pts are seen predominantly in the brain, and Western analysis shows a major peptide that migrates as

248 Western analysis shows here that gonococcal porin P.IB a

249 Western analysis shows that the native protein has the s

250 ssay, transcription of the target genes, and Western analysis studies indicated that the increased AP

251 ity in a concentration-dependent manner, and Western analysis suggested that PDP1 is directly phospho

252 uclear HO-1 and the faster migrating band on Western analysis, suggesting that this process was facil

253 Far-Western analysis suggests increased phosphorylation of p

254 rmed the expression of 9 of these genes, and Western analysis supported the conclusion that sulindac

255 was a reduction in COX2 protein levels using western analysis that corresponded with disease severity

256 Furthermore, we showed by Western analysis that the apparent age-dependent increas

257 We confirmed by Western analysis that the binding sites are separate usi

258 "pull-down" assay in which we identified, by Western analysis, the proteins in a rat kidney medullary

259 By immunofluorescence and western analysis, the transcription factor Hf1b/Sp4 and

260 By Western analysis they were found to be more abundant in

261 regulation were selected for verification by Western analysis to determine the reliability of the arr

262 In the current work, we performed Far-Western analysis to identify PfPP2C substrates.

263 owth conditions was examined by quantitative Western analysis to monitor the proteins present, their

264 Western analysis using a second phosphospecific antibody

265 P(3)Rs or InsP(3)R fragments was verified by Western analysis using antibodies cross-reacting with N-

266 Western analysis using antibodies recognizing epitopes t

267 Western analysis using antibody to MSH2, a key nuclear m

268 chment to cellular proteins was studied with Western analysis using antiserum raised against the benz

269 This expression pattern was confirmed by Western analysis using isoform specific polyclonal antib

270 Western analysis using mAb-174 revealed that the sizes o

271 NH2-terminal amino acid sequencing and Western analysis using monospecific antibodies to each t

272 osphorylated iNOS on Ser745 as determined by Western analysis using phospho-Ser antibody, in vitro ki

273 Two-dimensional gel analysis, Western analysis using phosphoamino acid-specific antise

274 ively collected gliomas through quantitative Western analysis using total and phospho-specific antibo

275 sis using a CaR-specific probe as well as by Western analysis utilizing a specific polyclonal anti-Ca

276 ng differential Triton X-100 solubility, and Western analysis was conducted to test for caveolae enri

277 Western analysis was conducted to validate and determine

278 solated from inner medullary tip or base and Western analysis was performed by use of an antibody to

279 ed by the stage of hook-basal-body assembly, Western analysis was performed on strains with mutations

280 Western analysis was used to assess extracellular signal

281 of endogenous SMAD messenger RNA (mRNA), and Western analysis was used to determine SMAD protein expr

282 Northern and/or Western analysis was used to determine the levels of gua

283 Endocardial NOS expression, determined by Western analysis, was also significantly decreased by 46

284 but the level of induction, as determined by western analysis, was dependent on the availability of Z

285 By western analysis we demonstrated that the HeLa extract c

286 By qRT-PCR and Western analysis we found that expression of Enpp1 was e

287 By Western analysis, we identified the 39-kd protein as Crk

288 -transcription polymerase chain reaction and Western analysis were used to determine if COX-2 express

289 R2B and GluR1 protein levels, as measured by Western analysis, whereas no changes were seen in mRNA e

290 expressed abundant levels of LRAT protein by Western analysis, whereas the RC cells did not express L

291 and sigma(G) proteins were not detectable by Western analysis, while sigma(F) protein levels were sig

292 Finally, Western analysis with a specific monoclonal antibody rev

293 Western analysis with an anti-phosphotyrosine antibody i

294 Western analysis with an antibody against human TRR prov

295 to peanut allergens was assessed by PAGE and Western analysis with anti-Ara h 1, 2, and 3 antibodies.

296 binding protein 1 (4EBP1) was measured using Western analysis with anti-phosphoantibodies.

297 Western analysis with anti-pTyr-STAT antibodies demonstr

298 rotein was shown to be identical to URB-1 by Western analysis with anti-URB-1 antibody.

299 Using western analysis with two autophosphorylation-specific a

300 poptosis pathway activation was confirmed by Western analysis, with an antibody that recognizes cleav