ライフサイエンスコーパス: Western blot

1 tions of the TRIM proteins were confirmed by Western blot.

2 RGS2 than wild-type (WT) protein detected by Western blot.

3 of an opsonophagocytic inhibition assay and Western blot.

4 lected for confirmation by flow cytometry or Western blot.

5 tissue showed elevated 4-repeat isoforms by western blot.

6 munological comparison was carried out using Western blot.

7 Selected proteins were validated by Western blot.

8 were assessed by histological techniques and western blot.

9 dings were confirmed at the protein level by Western blot.

10 tially only gp160 antibodies detected in the western blot.

11 arkers was evaluated by quantitative PCR and Western blot.

12 p-STAT3 levels were measured using Western blot.

13 umors using immunohistochemical staining and western blot.

14 e monitored by qPCR, immunocytochemistry and western blots.

15 ier tests are higher-specificity IgG and IgM Western blots.

16 tier tests sequentially, without the need of Western blots.

17 nal 2-tiered testing, obviating the need for Western blots.

18 men, prevented seroconversion, as shown with western blotting.

19 at protein level by confocal microscopy and western blotting.

20 ne A2 (TP) receptor expression determined by Western blotting.

21 ike receptor 4 (TLR-4) protein expression by Western blotting.

22 ed by phosphorylation of Chk1 and H2AX using Western blotting.

23 MG glycation was analysed, and confirmed by Western blotting.

24 ADAMTS13 antibody was detected by Western blotting.

25 of matrix (LYPAL), by mass spectrometry and Western blotting.

26 ns were detected by immunohistochemistry and western blotting.

27 y quantitative polymerase chain reaction and Western blotting.

28 NK were measured by immunohistochemistry and Western blotting.

29 of Bcl-2 and Bax, respectively, revealed by Western blotting.

30 ry factors were measured by qPCR, ELISA, and Western blotting.

31 nti-PDE10A antibody immunohistochemistry and Western blotting.

32 pond to chemokine gradients was supported by Western blotting.

33 A3 in blood-stage parasites was confirmed by western blotting.

34 sity were selected for further validation by western blotting.

35 e nitric oxide synthase levels determined by Western blot, 3-nitrotyrosine and 4-hydrpxnonenal both a

36 RNA(+) and an additional 4% were positive on western blot alone.

37 qPCR,luciferase reporter assays, and western blot also verified the ATP synthase subunit gene

38 ng human P2X7R (HEK293-hP2X7R) and underwent Western blotting, an immunofluorescence assay, and radio

39 Western blot analyses confirmed the involvement of Sirt2

40 ioinformatic, luciferase reporter assay, and Western blot analyses identified RhoA as a target of miR

41 ting results with flow cytometry, ELISA, and Western blot analyses in an additional 39 patients and 2

42 Quantitative RT-PCR and western blot analyses indicated that both Piezo1 and Pie

43 Western blot analyses indicated that p38a, STAT1, STAT3,

44 Immunohistochemical staining and Western blot analyses showed increased EZH2 expression i

45 Western blot analyses showed that the loss of LKB1 and p

46 amis) - were compared by electrophoretic and western blot analyses to identify biomarkers for BT.

47 Immunohistologic and Western blot analyses were performed to evaluate NIS pro

48 and immunofluorescent stainings, as well as Western blot analyses, on cervical and lumbar sections o

49 owed by sacrifice and immunohistochemical or western blot analyses.

50 as assessed by immunofluorescence assays and Western blot analyses.

51 hours after portal reperfusion, followed by Western blot analyses.

52 Sixty patient samples underwent Western blot analysis (15 young, 24 aged, and 21 with AD

53 ent cohorts for the presence of THSD7A-Ab by Western blot analysis and a newly developed indirect imm

54 lar mediators of autophagy was measured with Western blot analysis and fluorescence microscopy.

55 Western blot analysis and gene expression profiling show

56 human plasma and Lp(a) uptake studied using Western blot analysis and intracellular localization of

57 Targets of miRNA-31-3p were confirmed by Western blot analysis and luciferase reporter assay.

58 Here we used far-Western blot analysis and microscale thermophoresis to s

59 Quantitative PCR and western blot analysis confirmed loss of Bach2 in c-Rel m

60 Western blot analysis confirmed loss WIF1 expression and

61 Western blot analysis confirmed the increase in GluA1 ex

62 Signaling study with specific inhibitors and Western blot analysis demonstrated that CTGF-induced exp

63 Furthermore, western blot analysis demonstrated that LCN stimulations

64 Western blot analysis demonstrated UT-A1 in the inner me

65 Western blot analysis indicated that several autophagy-r

66 Western blot analysis indicated that the protein express

67 Western blot analysis indicated that Wnt7a induced both

68 The Western blot analysis indicates the downstream effectors

69 Isoelectric focusing followed by Western blot analysis of bleached isolated retinae showe

70 ssociated beta-Galactosidase staining and by Western blot analysis of p16.

71 peaked by 72 h on immunohistochemistry, and Western blot analysis of P2X7R for saline- and lipopolys

72 On Western blot analysis of pathway checkpoints, p-mTOR (p=

73 Western blot analysis of the basolateral amygdala (BLA)

74 Western blot analysis of total cell lysate obtained from

75 Likewise, western blot analysis revealed a 41% clearance of solubl

76 Although Western blot analysis revealed no difference in opsin ex

77 Western blot analysis revealed that mTOR deletion led to

78 Western blot analysis revealed that Notch2HCS mutant spl

79 Western blot analysis showed that both phosphorylated ST

80 Western blot analysis showed that IL28B TT genotype hepa

81 In particular, live cell Ca(2+) imaging and Western blot analysis showed that TRAM-34 pretreatment d

82 we employed immunofluorescence staining and Western blot analysis to assess the expression of autoph

83 s shown by pathway inhibitor experiments and Western blot analysis to be mediated via MEK-ERK signali

84 This study used western blot analysis to compare protein levels of tyros

85 macologic inhibitors, phosphoproteomics, and Western blot analysis to define the necessity of various

86 w conditions coupled with flow cytometry and Western blot analysis to elucidate the function and stru

87 Cellular fractionation and Western blot analysis were used to measure the levels of

88 was assessed by immunofluorescence staining, Western blot analysis, and ELISA.

89 ly transfected cells was examined by RT-PCR, western blot analysis, and immunohistochemistry.

90 Western blot analysis, as well as characterization of th

91 y, the level of target protein expression by Western blot analysis, platelet aggregation using an agg

92 llected January 2007 to March 2014) included Western blot analysis, pulsed-field gel electrophoresis

93 tion, as determined by luciferase assays and Western blot analysis, reversion-inducing cysteine-rich

94 Compared with Western blot analysis, the IFT had a 92% sensitivity and

95 In particular, we examined, using Western blot analysis, the levels of H3K36me3 and H4K16a

96 Using Western blot analysis, we confirmed that treatment of HS

97 Through western blot analysis, we identified that human glioma c

98 ssed by surface biotinylation and subsequent Western blot analysis.

99 mples from human and mouse models of DCM via Western blot analysis.

100 or the presence of antiretinal antibodies by Western blot analysis.

101 protein of the expected molecular weight in western blot analysis.

102 amino acid transporter 3) were validated by Western blot analysis.

103 ibition of EGFR downstream signalling in the western blot analysis.

104 tative RT-PCR, and proteins were detected by Western blot analysis.

105 GluA1-S845A mutant mice were compared using western blot analysis.

106 ations, as measured by two-dimensional (2-D) Western blot analysis.

107 LC26A6 in duodenum by immunofluorescence and Western blot analysis.

108 mLIFR), microarray expression profiling and Western blotting analysis identified preferential phosph

109 Western blotting analysis of PELP1-cyto HMECs showed up-

110 A Western blotting analysis revealed that biotin-labeled b

111 s, real-time quantitative PCR (RT-qPCR), and Western blotting analysis.

112 ty and secondary structure were confirmed by Western Blot and Circular Dichroism (CD), respectively.

113 sing cell-surface cross-linking, followed by Western blot and coimmunoprecipitation.

114 eened for viral oncoprotein expression using western blot and dot blot.

115 OPN expression was confirmed by Western blot and ELISA, and OPN was purified using Ni af

116 ic treatments under sonication, by SDS-PAGE, western blot and ELISA, with serum IgE of allergic indiv

117 eceptor-mediated cell signaling, such as the western blot and flow cytometry, are limited in three as

118 igase small interfering RNA library based on western blot and identified SCF-FBXO32 to be a new E3 li

119 IgE-western blot and IgE-ELISA were complemented by Skin Pri

120 Western blot and immunohistochemical analyses of heart t

121 Second, western blot and immunohistochemical analysis confirmed

122 l assay should have a 2-tier design and that Western blot and immunohistochemistry should be the meth

123 nhydrase XII (CA12), at the protein level by western blot and immunohistochemistry.

124 tumor versus muscle tissue as determined by Western blot and immunohistochemistry.

125 Real time PCR, western blot and knock down assays demonstrate that IL-2

126 ating cell nuclear antigen were evaluated by Western Blot and liver injury was assessed by ALT and hi

127 utoantigen, defined in mouse brain lysate by Western blot and mass spectrometry, was confirmed by dua

128 Western blot and mRNA expression analyses in mice yielde

129 Using western blot and quantitative real-time PCR analysis, ou

130 Western blot and RT-PCR were performed to quantitate Hsp

131 The MRM UHPLC-MS/MS method, Western blot and RT-PCR were used along with SULT activi

132 We confirmed, using western blot and spectrophotometry, that Hsp90 or BRAF i

133 R population, which was further confirmed by western blot and/or qPCR.

134 Western blots and ELISA were used to evaluate immunoreac

135 antly expressed N-terminal LRP2 fragments on Western blots and immunoprecipitated the recombinantly e

136 Western blots and proteomic analysis corroborated the in

137 t chain 3 (LC3), an autophagosome marker, by Western blotting and confocal fluorescence microscopy.

138 r electrophysiological characterization, and Western blotting and confocal imaging to assay expressio

139 urse studies and analyzed via Iba1 and P2X7R Western blotting and Iba1 immunohistochemistry.

140 Western blotting and immunofluorescence were used for ev

141 ctivity was determined by ELISA, zymography, Western blotting and immunofluorescent staining.

142 Grx1 and Trx1 levels as reflected by RT-PCR, Western blotting and immunohistochemistry analyses.

143 Western blotting and immunostaining analysis confirmed t

144 depletion of carbon sources, enzyme assays, Western blotting and mass spectrometric analysis to moni

145 Western blotting and molecular docking studies suggested

146 Western blotting and MS results indicated that rHBeAg an

147 Western blotting and quantitative PCR analysis of infect

148 Western blotting and Real-time PCR were used to assess t

149 tion, luciferase assays, immunofluorescence, western blotting and virus infection.

150 pendent LSE samples (quantitative RT-PCR and Western blotting) and in normal and AE skin biopsy speci

151 ivo analysis was performed via RNA analysis, Western blot, and histology.

152 rse transcriptase-polymerase chain reaction, Western blot, and immunohistochemistry were used to anal

153 e provided by chromatin immunoprecipitation, Western blot, and immunostaining.

154 y which was confirmed with luciferase assay, western blot, and NF-kappaB analysis.

155 Immunohistochemistry, Western blot, and qRT-PCR were performed on the outflow

156 NA-Seq), reverse transcription-PCR (RT-PCR), Western blot, and secretome analyses established that S.

157 Our blocking studies, western blot, and tissue histological imaging suggested

158 lated approximately 41-kDa protein (spx1) on Western blots, and the signal was efficiently blocked by

159 centrifugation, immunoprecipitation, dot and Western blotting, and confocal imaging were performed fo

160 antitative electron microscopic morphometry, Western blotting, and functional tests.

161 istance, paracellular flux, mRNA expression, Western blotting, and immunofluorescence staining.

162 eta was evaluated by means of real-time PCR, Western blotting, and immunohistochemistry.

163 detected by fluoroenzyme immunoassay, immuno-Western blotting, and line-blot immunoassay.

164 rgans were sampled for immunohistochemistry, western blotting, and mitochondrial high-resolution resp

165 ingle B cells were evaluated by using ELISA, Western blotting, and peptide epitope mapping.

166 ungs were assessed using immunofluorescence, Western blotting, and reverse transcriptase-polymerase c

167 racellular pathways was assessed by means of Western blotting, and the final outcome on immunomodulat

168 rse transcription polymerase chain reaction, Western blotting, and zymography.

169 nosorbent assay (ELISA), flow cytometry, and Western blot are common bioanalytical techniques.

170 Western blots are also complex, difficult to interpret,

171 Western blots are also usually used to measure only one

172 Thus, multiplexed Western blots are possible without cumbersome stripping

173 Src) led to an increase in monomeric Cav1 in Western blots, as well as a simultaneous increase in ves

174 zyme-linked immunosorbent assay (ELISA), and Western blot assay.

175 Further analyses using western blot assays showed that the FOXA2 p.(S169P) vari

176 In Western blot assays, we showed thatL.

177 using 3'-UTR luciferase reporter assays and Western blot assays.

178 functional assays were performed, including Western blot, cell migration, glucose transporter, and h

179 ding microarray, quantitative real-time PCR, Western blotting, chromatin immunoprecipitation, and fun

180 The in vivo fluorescence observations and Western blots confirmed MzASMT9 was localized in the chl

181 Quantitative PCR (qRT-PCR) and Western blotting confirmed changes in expression of seve

182 potent coactivator of ACTA2 Two-dimensional Western blotting confirmed induction of both wild-type a

183 ptase-polymerase chain reaction (RT-PCR) and Western blotting, confirming a concentration-dependent i

184 ield superior affinity reagents suitable for western blotting, confocal fluorescence microscopy and p

185 The Western blot data shown for p-ERK1/2 and actin are not f

186 Size exclusion chromatography and Western blotting data obtained by using purified recombi

187 Flow cytometry and Western blot demonstrated that lipoglycans from M. tuber

188 ssessed using pharmacologic manipulation and western blot detection of serotonin receptors, measureme

189 ronchial fibroblasts and characterized using Western blot, electron microscopy and flow cytometry.

190 We used qRT-PCR, Western blotting, ELISA, and ChIP (chromatin immunopreci

191 cular genetic experiments (quantitative PCR, Western blot, EMSA) or genome-wide assays (RNA-sequencin

192 ssion of GATA4 in nucleus were detected with Western blot experiment.

193 are evaluated in ELISA, flow cytometry, and Western blot experiments and compared to analogous exper

194 unohistochemistry, RT-PCR, northern blot and western blot experiments.

195 serum as well as for its detection by ELISA, Western blot, flow cytometry, and confocal microscopy.

196 Here we describe a microfluidic western blot for an eight-plex protein panel for individ

197 Subsequently, Western blot for the autophagic structure LC3-I and LC3-

198 eased LC3b-II/LC3b-I ratio, were detected by Western blotting from whole retinal extracts.

199 pression and traffic by confocal microscopy, western blotting, gel filtration, and immunoprecipitatio

200 inding assay (VOPBA) in combination with far-Western blotting, gradient-purified EAV particles were s

201 enzyme-linked immunosorbent assays (ELISA), Western blot, high performance liquid chromatography (HP

202 In the present study, Western blotting identified a band corresponding to C3 i

203 nasal polyp tissue were analyzed by means of Western blotting, immunoassays, or histology.

204 HCV-induced autophagosomes was confirmed by Western blotting, immunofluorescence microscopy, and imm

205 ctions were validated by immunoprecipitation/Western blotting, immunofluorescence, and proximity liga

206 Using western blotting, immunofluorescence, ELISA and qRT-PCR,

207 We used western blots, immunohistochemistry, H&E and biochemical

208 es were established and then validated using western blotting, immunohistochemistry, and comparable T

209 Using molecular cloning, RT-PCR, Western blotting, immunolocalization and in vitro functi

210 Real-time polymerase chain reaction, (far) Western blot, immunoprecipitation, and immunocytochemist

211 1 active dimer and ENG were analyzed also by Western blot in ascending aorta samples from other 10 tr

212 erase assays, quantitative real-time PCR and western blots in vitro and in vivo.

213 were generated and verified using q-PCR and western blotting in human keratinocytes (HaCaT) and endo

214 munohistochemistry on kidney sections and by Western blotting in plasma samples from rats subjected t

215 We demonstrate their superior performance in Western blotting, in both peroxidase- and fluorophore-li

216 lymerase chain reaction, flow cytometry, and Western blotting-in several nonprostatic cell lines and

217 its major partners by mass spectrometry and western blotting, including DDX6, UNR, unrip, PAT1B, LSM

218 Western blotting indicated that NMII-induced B1a cell de

219 RNA-treated cells by electron microscopy and Western blotting indicated that PI3K-C2A was not require

220 However, direct quantification via western blots indicates that the actin expression is the

221 Western blotting is a commonly used protein assay that c

222 Western blotting is most often used for protein identifi

223 tometry lysosome compartment evaluation, and western blotting light chain 3 (microtubule-associated p

224 Parallel western blot, luciferase reporter and gene target assays

225 ctivator, and further confirmed by RT-q-PCR, western blot, luciferase reporter, and ChIP assays.

226 imates which survived EBOV challenge, ELISA, western blot, mass spectrometry and flow cytometry were

227 sphorylation by quantitative Western and far-Western blotting, mass spectrometry, and computational m

228 a immunohistochemistry and HIF-1alpha/2alpha Western blot/messenger RNA analysis of inflamed and heal

229 sing quantitative real time PCR (n = 21) and western blotting (n = 9).

230 Casein zymography and Western blot of m-calpain were performed using the water

231 Western blotting of MKK2 protein and its T31 phosphoryla

232 To address this question, we used Western blotting of postmortem tissue from human V1 (12

233 A dose-dependent MGMT silencing confirmed by Western blotting of tumor tissue ex vivo.

234 ydrase I and S100A8 with ECM was verified by western blot on MP from DBA/1 and C57BL/6 mice.

235 Western-blotting on mouse cervix confirmed large scale h

236 the sample either being positive on standard western blot or harboring HDV RNA detectable by real-tim

237 hologically confirmed individuals with AD by Western blot or immunofluorescence for AQP4, amyloid-bet

238 gene expression as judged by flow cytometry, Western blot or immunofluorescence.

239 -PCR, ELISA (IL-6, IL-1beta, and CXCL2), and Western blot or immunostaining (CEBP, STAT3, NUPR1, and

240 brains or spleen of these animals by either Western blotting or after amplification by serial PMCAb.

241 y quantitative label-free mass spectrometry, Western blotting, or confocal imaging.

242 wed by immunoglobulin M and immunoglobulin G Western blots, performs well in late-stage LD but is ins

243 n experiments with knockdown of GAB2 and Far-Western blots proved the direct interaction of SHP2 with

244 arge cohort of human brain tissue samples by Western blotting, quantitative PCR, and immunohistochemi

245 late to RTK expression change as assessed by Western blot (R(2) of 0.85-0.98).

246 unctional outcomes were assessed by means of Western blotting, real-time PCR, differentiation, and pr

247 cell cycle analysis, morphologic assessment, Western blotting, receptor binding, gene expression, sma

248 ination of Gel filtration chromatography and Western blot, respectively.

249 oteins were detected by real-time RT-PCR and western blots, respectively.

250 d thrombin levels were measured by ELISA and Western blotting, respectively, in blood samples from 10

251 n were detected with quantitative RT-PCR and Western blotting, respectively, in both platelets and Me

252 correlated to immunohistochemistry findings, Western blot results, and results of myography.

253 Immunofluorescence microscopy and Western blotting results showed that RSV infection of hu

254 Immunofluorescence microscopy and western blotting revealed marked disorganization and red

255 IHC and western blotting revealed reduction in epidermal hyperpl

256 Western blotting revealed the presence of Pph (Ptb33) an

257 (n = 11) with real-time quantitative PCR and Western blotting revealed up-regulation of C3 and CFB ex

258 analysis, supplemented by real-time PCR and Western blotting, revealed that the expression of critic

259 ng were determined using immunofluorescence, western blot, reverse-transcriptase PCR, chromatin immun

260 ciferase assays, quantitative real-time PCR, western blots, scratch assays, CCK-8 assays and tubule f

261 e conventional western blotting, single-cell western blotting (scWB) is particularly useful for prote

262 eas only 5 of 29 (17%) patients had positive Western blot seroreactivity.

263 ng protein peptides), but quantitation using western blots showed 6.3- to 8-fold or 91- to 125-fold i

264 age-matched non-transgenic littermates, and western blots showed increased lysosomal proteins includ

265 Western blots showed that the triceps surae muscles of '

266 Like conventional western blotting, single-cell western blotting (scWB) is

267 RNA sequencing, quantitative real-time PCR, Western blotting, small interfering RNA interference, an

268 ted mRNA and the severe loss of calpain 3 on western blotting, suggest a dominant negative effect wit

269 Western blotting suggested beta-conglutins were the main

270 ile upstream regulator analysis coupled with Western blotting suggests that abnormal basal activation

271 Using mass spectrometry and Western blotting techniques, we have identified choline

272 both antigen and antibody to 65 days for the Western blot test.

273 ally, we confirmed by immunofluorescence and western-blot that rod degeneration in CERKL-/- zebrafish

274 d quantitative polymerase chain reaction and Western blotting to investigate changes in ion channel e

275 The major antigenic region was defined by Western blot using recombinant protein fragments.

276 and Cry1AcMod binding and oligomerization by western blots using brush border membrane vesicles (BBMV

277 Abeta oligomers, whereas immunoprecipitation-Western blotting using high-detergent extracts revealed

278 mmunoprecipitation and simultaneous assay by Western blotting using multiplex fluorescence imaging wi

279 These were further validated by Western blotting using specific Abs.

280 d mass spectrometry, and further verified by Western blotting using specific Abs.

281 specific for all five tested SULTs, whereas Western blot was specific for only two isoforms.

282 At the same time Western blotting was performed with the patient's serum

283 ansient transfection with miR-146b mimic and western blotting was used to analyse SOX5.

284 vels were determined by neutral comet assay; western blotting was used to evaluate protein changes; c

285 rent commercial sources were investigated by western blot (WB) and in multiple research grade PSA ELI

286 TJ protein expression was assessed by Western blotting (WB) and immunocytochemistry (ICC).

287 isease-associated prion protein (PrP(Sc)) by Western blotting (WB), antigen capture enzyme immunoassa

288 MS) or on immunochemical techniques, such as western blotting (WB).

289 Based on lipid analysis and Western blotting, we show that the bacteria-like entitie

290 mmunohistochemistry, immunofluorescence, and Western blot were used to validate apoptosis by cleaved

291 Western blots were used to quantify the abundance of the

292 d its receptor (GHRH-R) measured by qPCR and Western blotting were significantly down-regulated in re

293 Microscopy and Western blotting were used to determine activation of si

294 Real-time polymerase chain reaction and Western blotting were used to evaluate Renal Klotho expr

295 ctivity, and m-calpain level (as assessed by Western blot) were all significantly higher in the NACA-

296 All patients had antiretinal antibodies on Western blot with an average of 6.6 bands.

297 ified by 2D gel electrophoresis, followed by Western blot with pooled or individual sera.

298 Allergen detection in 2D-Western blots with PBS resulted in greater sensitivity t

299 We performed Western blotting with enokitake extracts and the patient

300 ), cases (n = 20)) by automated quantitative western blotting, with excellent agreement with our prot