ライフサイエンスコーパス: Western blotting

1 ns were detected by immunohistochemistry and western blotting.

2 y quantitative polymerase chain reaction and Western blotting.

3 NK were measured by immunohistochemistry and Western blotting.

4 uman serum samples was detected by ELISA and Western blotting.

5 e interactions by co-immunoprecipitation and Western blotting.

6 hlorous acid (HOCl)-induced SSc by ELISA and Western blotting.

7 scle biopsy and loss of calpain 3 protein on western blotting.

8 f activated T-cells (SOFAT) was evaluated by Western blotting.

9 and changes in Hsp levels were determined by Western blotting.

10 could be detected in CCD case cerebellum by western blotting.

11 of Bcl-2 and Bax, respectively, revealed by Western blotting.

12 target genes, were determined by RT-qPCR and Western blotting.

13 the phosphorylation of p70 S6 kinase, using Western blotting.

14 VHL was often detected as multiple bands by western blotting.

15 ls of key pathway proteins were validated by western blotting.

16 rexpression by exome sequencing, RT-PCR, and Western blotting.

17 irmation of repeat reactive samples by HIV-1 Western blotting.

18 lly, and hypoxia-inducible factor 1-alpha by Western blotting.

19 ry factors were measured by qPCR, ELISA, and Western blotting.

20 and GRK2 phosphorylation was measured using Western blotting.

21 th thylakoids exposed to Mn deficiency after western blotting.

22 cted with a mouse anti-BmAMA1 antibody using Western blotting.

23 nti-PDE10A antibody immunohistochemistry and Western blotting.

24 gene assay, immunofluorescent staining, and Western blotting.

25 in expression were successfully measured via Western blotting.

26 .Arg590*, p.Glu134* and Delta exons 9-14) by western blotting.

27 o-dimensional proteomics and two-dimensional Western blotting.

28 5 nuclear translocation were quantified with Western blotting.

29 d the proteins of interest were validated by Western blotting.

30 A3 in blood-stage parasites was confirmed by western blotting.

31 ment for cell lysis, gel electrophoresis and western blotting.

32 subunit of the AMPA receptor, measured with western blotting.

33 es were validated by quantitative RT-PCR and Western blotting.

34 ric oxide synthase pathways were assessed by Western blotting.

35 and Ser473) phosphorylation was analyzed by Western blotting.

36 ession and cellular localization examined by Western blotting.

37 stantiated with protein expression levels by Western blotting.

38 staining, elastica van Gieson staining, and Western blotting.

39 histone arginine methylation was assessed by Western blotting.

40 -transcriptase polymerase chain reaction, or western blotting.

41 selected proteins were confirmed by means of Western blotting.

42 H89, as shown by immunoprecipitation-coupled Western blotting.

43 pond to chemokine gradients was supported by Western blotting.

44 sity were selected for further validation by western blotting.

45 men, prevented seroconversion, as shown with western blotting.

46 at protein level by confocal microscopy and western blotting.

47 ne A2 (TP) receptor expression determined by Western blotting.

48 ike receptor 4 (TLR-4) protein expression by Western blotting.

49 ed by phosphorylation of Chk1 and H2AX using Western blotting.

50 MG glycation was analysed, and confirmed by Western blotting.

51 ADAMTS13 antibody was detected by Western blotting.

52 of matrix (LYPAL), by mass spectrometry and Western blotting.

53 endent kinase 2) were examined with qPCR and Western-blotting.

54 gel-shift assay, coimmunoprecipitation, and western blottings.

55 : 1) real-time polymerase chain reaction; 2) Western blotting; 3) fluorescence-activated cell sorting

56 Quantitative Western blotting allowed us to estimate that mouse thymo

57 ng human P2X7R (HEK293-hP2X7R) and underwent Western blotting, an immunofluorescence assay, and radio

58 , reverse transcriptase quantitative PCR and western blotting analyses, we confirm the direct regulat

59 mLIFR), microarray expression profiling and Western blotting analysis identified preferential phosph

60 Western blotting analysis of PELP1-cyto HMECs showed up-

61 A Western blotting analysis revealed that biotin-labeled b

62 the regenerative process, by real time PCR, Western blotting analysis, and immunohistochemistry.

63 quantified using both immunofluorescence and Western blotting analysis, and nuclear functional TDP-43

64 PKCalpha, but normal PKCzeta expression, by Western blotting analysis, and vice versa.

65 Here, using whole-cell patch clamp and Western blotting analysis, we demonstrate that the hERG/

66 s, real-time quantitative PCR (RT-qPCR), and Western blotting analysis.

67 isolated immunoreactive tryptic fragments by Western blotting and analyzed them by mass spectrometry.

68 t chain 3 (LC3), an autophagosome marker, by Western blotting and confocal fluorescence microscopy.

69 r electrophysiological characterization, and Western blotting and confocal imaging to assay expressio

70 Western blotting and cytochemistry used Siglec-F-Fc as a

71 ippocampus by using immunohistochemistry and western blotting and employing high-resolution fluoresce

72 ed both in vitro by using flow cytometry and Western blotting and ex vivo by using RT-PCR after laser

73 Western blotting and fluorescent resonance energy transf

74 atric comparison subjects was analyzed using Western blotting and Golgi histochemistry to examine the

75 tion polymerase chain reaction (qRT-PCR) and western blotting and hepatic stellate cell (HSC)-MF cont

76 urse studies and analyzed via Iba1 and P2X7R Western blotting and Iba1 immunohistochemistry.

77 Western blotting and immunofluorescence analysis demonst

78 arget phosphorylation, as identified through Western blotting and immunofluorescence microscopy.

79 The effect of AZD6738 was assessed by western blotting and immunofluorescence of key DDR prote

80 Western blotting and immunofluorescence revealed S1P1 an

81 Western blotting and immunofluorescence were used for ev

82 ere detected by means of immunoprecipitation/Western blotting and immunofluorescence.

83 ctivity was determined by ELISA, zymography, Western blotting and immunofluorescent staining.

84 Grx1 and Trx1 levels as reflected by RT-PCR, Western blotting and immunohistochemistry analyses.

85 Western blotting and immunohistochemistry of the explant

86 Western blotting and immunohistochemistry showed decreas

87 qPCR, Western blotting and immunohistochemistry were performed

88 noreactivity in the hippocampus, measured by Western blotting and immunohistochemistry, was increased

89 Using a combination of Western blotting and immunohistochemistry, we identified

90 ction of STAT3 and NFkappaB were detected by Western blotting and immunoprecipitation, respectively.

91 Western blotting and immunostaining analysis confirmed t

92 E9 trigeminal ganglia were identified using Western blotting and immunostaining.

93 after ONC was determined via combined use of western blotting and immunostainings.

94 ence microscopy of isolated nucleoplasts and Western blotting and MALDI-TOF of nuclear extracts show

95 depletion of carbon sources, enzyme assays, Western blotting and mass spectrometric analysis to moni

96 Western blotting and molecular docking studies suggested

97 Western blotting and MS results indicated that rHBeAg an

98 Western blotting and PCR approaches specifically showed

99 Western blotting and quantitative PCR analysis of infect

100 Immunohistochemistry, Western blotting and quantitative RT-PCR analyses reveal

101 Western blotting and Real-time PCR were used to assess t

102 Western blotting and reverse transcription-PCR indicated

103 Tumor signaling was studied by Western blotting and reverse-phase protein array analysi

104 The expression states were determined by Western blotting and two genes, fetA and nadA, exhibited

105 tion, luciferase assays, immunofluorescence, western blotting and virus infection.

106 MPRIN in gingival tissues were examined with Western blotting and zymography, respectively.

107 ion and caused cell cycle arrest as shown in Western blottings and flow cytometry assays utilizing bo

108 pendent LSE samples (quantitative RT-PCR and Western blotting) and in normal and AE skin biopsy speci

109 All 33 mutants were detected by Western blotting, and 28 were active for [(3)H]methotrex

110 PCR arrays, in silico analysis, Western blotting, and 3'-UTR luciferase reporter assays

111 y using flow cytometry, confocal microscopy, Western blotting, and a quantitative PCR array.

112 acetylation of SacAcsA enzyme was studied by Western blotting, and acetylation of lysine residues Lys

113 n microtiter plates, by two-dimensional (2D) Western blotting, and by surface plasmon resonance demon

114 centrifugation, immunoprecipitation, dot and Western blotting, and confocal imaging were performed fo

115 elial cells was monitored by flow cytometry, Western blotting, and confocal/electron microscopy.

116 d by quantitative polymerase chain reaction, Western blotting, and DNA binding assays (for transcript

117 qRT-PCR, Western blotting, and enzymatic assays were performed to

118 ing protein sialoforms by mass spectrometry, Western blotting, and flow cytometry.

119 antitative electron microscopic morphometry, Western blotting, and functional tests.

120 was examined by quantitative real-time PCR, Western blotting, and immunofluorescence in human alveol

121 istance, paracellular flux, mRNA expression, Western blotting, and immunofluorescence staining.

122 expression, as examined by real-time RT-PCR, Western blotting, and immunofluorescence.

123 d by quantitative reverse transcriptase-PCR, western blotting, and immunohistochemistry in CTCL cell

124 Polymerase chain reaction, Western blotting, and immunohistochemistry were used to

125 eta was evaluated by means of real-time PCR, Western blotting, and immunohistochemistry.

126 tis were analyzed by using quantitative PCR, Western blotting, and immunohistochemistry.

127 and MUC1 CT was evaluated by real-time PCR, Western blotting, and immunohistochemistry.

128 rse transcriptase polymerase chain reaction, Western blotting, and immunohistochemistry.

129 miRNA and mRNA arrays, RT-quantitative PCR, Western blotting, and immunonohistochemistry.

130 detected by fluoroenzyme immunoassay, immuno-Western blotting, and line-blot immunoassay.

131 infected cells, followed by silver staining, Western blotting, and mass spectrometry, identified the

132 were processed for histological evaluation, Western blotting, and metabolomics analyses using UPLC-Q

133 rgans were sampled for immunohistochemistry, western blotting, and mitochondrial high-resolution resp

134 ome characterization was performed with TEM, Western blotting, and NanoSight analysis.

135 ingle B cells were evaluated by using ELISA, Western blotting, and peptide epitope mapping.

136 ungs were assessed using immunofluorescence, Western blotting, and reverse transcriptase-polymerase c

137 and mRNA expression were analyzed by ELISA, Western blotting, and reverse-transcriptase polymerase c

138 ted genes were identified by immunostaining, Western blotting, and RNA analysis.

139 racellular pathways was assessed by means of Western blotting, and the final outcome on immunomodulat

140 By far-Western blotting, and verified by coimmunoprecipitation,

141 rse transcription polymerase chain reaction, Western blotting, and zymography.

142 ) phospho-specific mass spectrometry; 2) far-Western blotting; and 3) live cell single-molecule imagi

143 Several assays, including Western blotting, annexin-V/propidium iodide binding, co

144 u cerebral perfusion, histological analysis, Western blotting, as well as transmission and scanning e

145 In addition, H&E staining, TUNEL and Western blotting assays were performed in order to obser

146 nvestigated by histological confirmation and western blotting at 24h, 3d, 7d and 14d, respectively.

147 Western blotting, chromatin immunoprecipitation (ChIP),

148 ding microarray, quantitative real-time PCR, Western blotting, chromatin immunoprecipitation, and fun

149 accumbens and dorsal striatum of rats using western blotting, co-immunoprecipitation, and chromatin

150 Quantitative PCR (qRT-PCR) and Western blotting confirmed changes in expression of seve

151 potent coactivator of ACTA2 Two-dimensional Western blotting confirmed induction of both wild-type a

152 Reverse transcription-PCR (RT-PCR) and Western blotting confirmed that genes involved in DR-ind

153 Western blotting confirmed the enhanced protein levels o

154 try, polymerase chain reaction analysis, and Western blotting confirmed the presence of apical NaPi-2

155 Slc26a6 in mouse incisor enamel organs, and Western blotting confirmed their translation into protei

156 ptase-polymerase chain reaction (RT-PCR) and Western blotting, confirming a concentration-dependent i

157 ield superior affinity reagents suitable for western blotting, confocal fluorescence microscopy and p

158 Western blotting consistently detected 50% BSE within a

159 Size exclusion chromatography and Western blotting data obtained by using purified recombi

160 Molecular screening using qRT-PCR and Western blotting demonstrated that aldosterone treatment

161 Cell fractionation and Western blotting demonstrated that beta-catenin fragment

162 Western blotting demonstrated that chronic hyperglycemia

163 Western blotting (Dot Blot) as an immunological method c

164 We used qRT-PCR, Western blotting, ELISA, and ChIP (chromatin immunopreci

165 of misfolded CWD prion protein (PrP(CWD)) by western blotting, ELISA, or immunohistochemistry (IHC).

166 sing of APP using co-immunoprecipitation and Western blotting/ELISAs, respectively.

167 expression and mRNA levels were analyzed by Western blotting, flow cytometry, and real-time PCR.

168 evaluation of CHIKV protein expression using western blotting for CHIKV nsP1, nsP3, and E2E1 proteins

169 g using a SMAD-luciferase reporter assay and Western blotting for phospho-SMAD2/3 and ZEB-2 in cultur

170 arding induction of apoptosis, as studied by Western blotting for the cleaved fragment of poly(ADP-ri

171 eased LC3b-II/LC3b-I ratio, were detected by Western blotting from whole retinal extracts.

172 pression and traffic by confocal microscopy, western blotting, gel filtration, and immunoprecipitatio

173 issue insulin signaling protein abundance by Western blotting, glucose tolerance and utilization, and

174 inding assay (VOPBA) in combination with far-Western blotting, gradient-purified EAV particles were s

175 In the present study, Western blotting identified a band corresponding to C3 i

176 Phospho-arrays/Western blotting identified signalling activation in end

177 nasal polyp tissue were analyzed by means of Western blotting, immunoassays, or histology.

178 HCV-induced autophagosomes was confirmed by Western blotting, immunofluorescence microscopy, and imm

179 ctions were validated by immunoprecipitation/Western blotting, immunofluorescence, and proximity liga

180 Using western blotting, immunofluorescence, ELISA and qRT-PCR,

181 es were established and then validated using western blotting, immunohistochemistry, and comparable T

182 yzed by real-time polymerase chain reaction, western blotting, immunohistochemistry, and confocal mic

183 Using molecular cloning, RT-PCR, Western blotting, immunolocalization and in vitro functi

184 Western blotting, immunoprecipitation, and protein inhib

185 lial cells (GEnCs), quantitative PCR arrays, Western blotting, immunoprecipitation, immunofluorescenc

186 Muscle nNOS and PDE5 were tested with Western blotting in 5 patients.

187 ated (cleaved) C3 fragments were detected by Western blotting in extracts of IL-1alpha-stimulated car

188 were generated and verified using q-PCR and western blotting in human keratinocytes (HaCaT) and endo

189 gM, but not glycoprotein B, was detected by Western blotting in isolated axons during Us9-null PRV i

190 Western blotting in nelfinavir and its analog treated ce

191 munohistochemistry on kidney sections and by Western blotting in plasma samples from rats subjected t

192 Screen hits were validated with western blotting in the HDLM-2 Hodgkin's lymphoma cell l

193 We demonstrate their superior performance in Western blotting, in both peroxidase- and fluorophore-li

194 lymerase chain reaction, flow cytometry, and Western blotting-in several nonprostatic cell lines and

195 its major partners by mass spectrometry and western blotting, including DDX6, UNR, unrip, PAT1B, LSM

196 fter DETA-NO preconditioning was observed by Western blotting, including elevated phosphorylation of

197 Western blotting indicated that NMII-induced B1a cell de

198 RNA-treated cells by electron microscopy and Western blotting indicated that PI3K-C2A was not require

199 Western blotting is a commonly used protein assay that c

200 Western blotting is most often used for protein identifi

201 tometry lysosome compartment evaluation, and western blotting light chain 3 (microtubule-associated p

202 sphorylation by quantitative Western and far-Western blotting, mass spectrometry, and computational m

203 was confirmed by second dimension SDS-PAGE, Western blotting, mass spectrometry, and the use of a p1

204 m(2) vs 410 649 mum(2); P < .001) stains and western blotting (median, 2.01 vs 0.87; P = .009) demons

205 Here we present a modified Western blotting method that allows the rapid and reprod

206 sing quantitative real time PCR (n = 21) and western blotting (n = 9).

207 Western blotting of cross-linked SERCA revealed higher-m

208 Western blotting of MKK2 protein and its T31 phosphoryla

209 Western blotting of patient fibroblast and muscle showed

210 ffected members of the family as measured by Western blotting of platelet lysates.

211 To address this question, we used Western blotting of postmortem tissue from human V1 (12

212 Western blotting of rodent retina and brain homogenates

213 Using Western blotting of STN tissue punches, we demonstrated

214 A dose-dependent MGMT silencing confirmed by Western blotting of tumor tissue ex vivo.

215 d increased G-actin levels, as determined by Western blotting of vessel lysates, supporting involveme

216 This protocol describes how to perform western blotting on individual cells to measure cell-to-

217 Western-blotting on mouse cervix confirmed large scale h

218 anti-Ro/SSA-52-kD subtype detected by immuno-Western blotting only.

219 brains or spleen of these animals by either Western blotting or after amplification by serial PMCAb.

220 ve detection of glutathionylated proteins by Western blotting or fluorescence after click reaction wi

221 Biochemical processes were evaluated by Western blotting or immunofluorescence.

222 y quantitative label-free mass spectrometry, Western blotting, or confocal imaging.

223 duction in expression as measured by RT-PCR, Western blotting, or Luminex technology.

224 airpin RNA knockdown and ectopic expression, western blotting, PCR, promoter-luciferase reporter assa

225 es were unchanged during superinduction, yet Western blotting, pulse labeling, and the use of bicistr

226 teins were validated respectively by MRM and western blotting quantitative analyses.

227 arge cohort of human brain tissue samples by Western blotting, quantitative PCR, and immunohistochemi

228 ure within hours, and it can be performed on western blotting quantities of endogenously ubiquitinate

229 unctional outcomes were assessed by means of Western blotting, real-time PCR, differentiation, and pr

230 cell cycle analysis, morphologic assessment, Western blotting, receptor binding, gene expression, sma

231 d thrombin levels were measured by ELISA and Western blotting, respectively, in blood samples from 10

232 n were detected with quantitative RT-PCR and Western blotting, respectively, in both platelets and Me

233 Immunofluorescence microscopy and Western blotting results showed that RSV infection of hu

234 Immunofluorescence microscopy and western blotting revealed marked disorganization and red

235 IHC and western blotting revealed reduction in epidermal hyperpl

236 Western blotting revealed the presence of Pph (Ptb33) an

237 (n = 11) with real-time quantitative PCR and Western blotting revealed up-regulation of C3 and CFB ex

238 analysis, supplemented by real-time PCR and Western blotting, revealed that the expression of critic

239 correlated with total Cx40 quantification by Western blotting (rs=0.64, P<0.01, n=20).

240 e conventional western blotting, single-cell western blotting (scWB) is particularly useful for prote

241 Western blotting showed coelution of PM20D2 with beta-al

242 RT-quantitative PCR and Western blotting showed reduced levels of enzymes cataly

243 nfirmed through immunocytochemistry, whereas Western blotting showed that DDAH1 remained in the kidne

244 Western blotting showed that endogenous HMW PDE3A1 was t

245 Both RT-PCR and Western blotting showed that meayamycin B up-regulated M

246 Western blotting showed that oxytocin treatment blocked

247 er-driven luciferase reporter gene assay and Western blotting showed that recombinant Tat or Tat-cont

248 Western blotting showed that these channels also formed

249 Immunofluorescence staining and Western blotting showed the presence of UGT1A in the bas

250 Like conventional western blotting, single-cell western blotting (scWB) is

251 RNA sequencing, quantitative real-time PCR, Western blotting, small interfering RNA interference, an

252 ted mRNA and the severe loss of calpain 3 on western blotting, suggest a dominant negative effect wit

253 Western blotting suggested beta-conglutins were the main

254 ile upstream regulator analysis coupled with Western blotting suggests that abnormal basal activation

255 A triplex Western blotting technique was used to estimate the two

256 friendly assays based on combined microplate/Western blotting techniques that specifically detect eit

257 Using mass spectrometry and Western blotting techniques, we have identified choline

258 To conduct the Western Blotting test, recombinant MAF-1 fusion protein

259 hough reciprocally reactive with antisera by Western blotting, this truncated protein did not react w

260 We used Western blotting to examine the phosphorylation of Stat1

261 d quantitative polymerase chain reaction and Western blotting to investigate changes in ion channel e

262 Here, we use quantitative Western blotting to show that the PAS domain is not requ

263 nvestigated through gel electrophoresis with Western blotting, transmission electron microscopy, UV-V

264 ymes and the protein acetylation in cells by Western blotting (tubulin vs histone acetylation).

265 detected in mouse and human brain tissue by Western blotting using anti-huntingtin antibodies, we ex

266 Abeta oligomers, whereas immunoprecipitation-Western blotting using high-detergent extracts revealed

267 mmunoprecipitation and simultaneous assay by Western blotting using multiplex fluorescence imaging wi

268 These were further validated by Western blotting using specific Abs.

269 d mass spectrometry, and further verified by Western blotting using specific Abs.

270 dentified as beta-lactoglobulin (beta-LG) by Western blotting using specific monoclonal antibody and

271 Western blotting was performed to detect endogenous and

272 At the same time Western blotting was performed with the patient's serum

273 ansient transfection with miR-146b mimic and western blotting was used to analyse SOX5.

274 Western blotting was used to determine changes in leukoc

275 Western blotting was used to determine levels of IKKbeta

276 vels were determined by neutral comet assay; western blotting was used to evaluate protein changes; c

277 TJ protein expression was assessed by Western blotting (WB) and immunocytochemistry (ICC).

278 Western blotting (WB) indicated that PrP(263K), PrP(CWD)

279 Western blotting (WB) was employed to validate the downs

280 isease-associated prion protein (PrP(Sc)) by Western blotting (WB), antigen capture enzyme immunoassa

281 F were further studied using bioinformatics, Western blotting (WB), immunocytochemistry, and coimmuno

282 coating (TDC) has been successfully used in Western blotting (WB).

283 MS) or on immunochemical techniques, such as western blotting (WB).

284 y reduce the amount of antibody required for Western blotting (WB).

285 phorylated VEGF receptor 2 (pVEGFR2), and by western blotting we found that membrane VEGFR1 and VEGFR

286 Using real-time RT-PCR and Western blotting, we confirmed an up to 9-fold induction

287 Based on lipid analysis and Western blotting, we show that the bacteria-like entitie

288 d its receptor (GHRH-R) measured by qPCR and Western blotting were significantly down-regulated in re

289 Microscopy and Western blotting were used to determine activation of si

290 Real-time polymerase chain reaction and Western blotting were used to evaluate Renal Klotho expr

291 We have analyzed four commercial pAbs by Western blotting, which revealed prominent cross-reactiv

292 se chain reaction, immunohistochemistry, and Western blotting, while matriptase activity was monitore

293 In addition, Western blotting with convalescent rabbit serum detected

294 We performed western blotting with enokitake extract and the patient'

295 We performed Western blotting with enokitake extracts and the patient

296 hosphates as judged by mass spectrometry and Western blotting with phospho-specific antibodies.

297 Western blotting with Siglec-F-Fc detected approximately

298 d in the frontal cortex and cerebellum using Western blotting with specific antibodies.

299 This assignment was confirmed by Western blotting with whole-tooth crown and enamel extra

300 ), cases (n = 20)) by automated quantitative western blotting, with excellent agreement with our prot