ライフサイエンスコーパス: Y. enterocolitica

1 Y. enterocolitica biovar 1B additionally has a distinct

2 Y. enterocolitica cells are motile when grown at lower t

3 Y. enterocolitica infection promoted the development of

4 Y. enterocolitica infections in FoodNet sites have signi

5 Y. enterocolitica isolates recovered from the mice were

6 Y. enterocolitica lacking the virulence plasmid failed t

7 Y. enterocolitica mutants lacking either the Ysa or Ysc

8 Y. enterocolitica O:8 was isolated from 1 raw-milk sampl

9 Y. enterocolitica organisms were more virulent in the IL

10 Y. enterocolitica reduces S. Typhimurium invasion of HeL

11 Y. enterocolitica tends to persist in soil for long peri

12 Y. enterocolitica thus has three type III secretion path

13 Y. enterocolitica was also able to inhibit the invasion

14 Y. enterocolitica was present within the murine mucosa o

15 erence method was 1.2% ETEC, 0.1% Vibrio, 0% Y. enterocolitica, and 0% P. shigelloides Compared to th

16 Analysis of 100 Y. enterocolitica and Y. enterocolitica-like strains sho

17 From 1996 through 2009, 2085 Y. enterocolitica infections were reported to FoodNet.

18 he enteropathogenic E. coli strain E2348/69, Y. enterocolitica JB580, and Pseudomonas aeruginosa PAO1

19 .7% (99.4 to 99.8), and 0.96 (0.93 to 0.99); Y. enterocolitica, 99.0% (94.8 to 99.8), 99.9% (99.8 to

20 A Y. enterocolitica rovA mutant has a significant decrease

21 One role of IL-6 during a Y. enterocolitica infection may be the downmodulation of

22 In a Y. enterocolitica yenI mutant, swimming motility is temp

23 A striking feature of the pathology of a Y. enterocolitica infection is inflammation.

24 We previously reported the isolation of a Y. enterocolitica mutant (JB1A8v) that shows a decrease

25 of intestinal inflammation in response to a Y. enterocolitica infection.

26 Educating dairy owners about Y. enterocolitica and postpasteurization contamination i

27 rt antipathogenic effects in the gut against Y. enterocolitica and highlight the need to investigate

28 The most potent MP against Y. enterocolitica, methicillin-resistant S. aureus and M

29 Although Y. enterocolitica 0:8 strains are reported to have galac

30 common food pathogens, including E. coli and Y. enterocolitica, and could even detect Salmonella spp.

31 romotes serum resistance in both E. coli and Y. enterocolitica.

32 ose 4-epimerase activity in both E. coli and Y. enterocolitica.

33 Analysis of 100 Y. enterocolitica and Y. enterocolitica-like strains showed none to be within

34 , isolated 2,493 Yersinia enterocolitica and Y. enterocolitica-like strains, 22 Y. pestis strains, an

35 IV(A) modified with C16:0 predominated, and Y. enterocolitica produced a unique tetra-acylated lipid

36 ogenic for humans (Y. pseudotuberculosis and Y. enterocolitica).

37 ognate plasmids in Y. pseudotuberculosis and Y. enterocolitica, but their localization within the pla

38 umans (Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica) export and translocate a distinct set

39 sent in all 47 low-pathogenicity strains and Y. enterocolitica 8081 but absent from all nonpathogenic

40 occurring in response to S. typhimurium and Y. enterocolitica colonization of PP using Affymetrix Ge

41 l complementation of both S. typhimurium and Y. enterocolitica mutations and by DNA sequence analysis

42 hort interactions between S. Typhimurium and Y. enterocolitica or that the SdiA regulon members do no

43 rd to host cell uptake of S. Typhimurium and Y. enterocolitica, we investigated how each pathogen inf

44 However, because Y. enterocolitica is typically a food-borne pathogen, th

45 nt PspC destabilization is conserved between Y. enterocolitica and E. coli.

46 onserved mechanism of inv regulation between Y. enterocolitica and Y. pseudotuberculosis.

47 hich is positively regulated by RovA in both Y. enterocolitica and Yersinia pseudotuberculosis while

48 The RepA proteins encoded by Y. enterocolitica serotype 0:8 pYVeWA and pYVe8081 were

49 here early precursor B cells are expanded by Y. enterocolitica porins to undergo somatic hypermutatio

50 eveal the complete set of genes expressed by Y. enterocolitica in response to infection and provide t

51 ed to reverse the uptake blockade imposed by Y. enterocolitica.

52 erwise unable to block type III injection by Y. enterocolitica strains.

53 ntal signals are processed and integrated by Y. enterocolitica to stimulate the production of flagell

54 N-acylhomoserine lactones (AHLs) produced by Y. enterocolitica and upregulates the expression of an i

55 CopN, but not NrdB, was secreted by Y. enterocolitica in a Ca2+- and pYV-dependent fashion.

56 rison of Ysps with Yop effectors secreted by Y. enterocolitica indicated that YspG, YspH, and YspJ ha

57 the range of 30 degrees C and 37 degrees C, Y. enterocolitica phase-varies between motility and plas

58 ears to be provided by host cells and causes Y. enterocolitica to transport YopE and presumably other

59 were stimulated with flagella from E. coli, Y. enterocolitica, and P. aeruginosa in the presence of

60 active surveillance for laboratory-confirmed Y. enterocolitica infections, defined as the isolation o

61 e H(1) receptor is important for controlling Y. enterocolitica infection within the Peyer's patches a

62 ase TaqMan PCR assay was developed to detect Y. enterocolitica in blood.

63 degrees C was also derepressed in a directed Y. enterocolitica clpP mutant.

64 ting protective roles for CD4 T cells during Y. enterocolitica infection, vaccinating mice with a 16-

65 bitory role of this endogenous lectin during Y. enterocolitica infection.

66 ith a regulatory role for this lectin during Y. enterocolitica pathogenesis, mice lacking Gal-1 showe

67 IL-6 plays an anti-inflammatory role during Y. enterocolitica infection, and in other systems IL-6 h

68 A gene encoding Y. enterocolitica phospholipase was identified, and anal

69 as medium values decreased below pH 3.0 for Y. enterocolitica and pH 5.5 for M. morganii, suggesting

70 ally informative typing scheme available for Y. enterocolitica.

71 that the RovA regulon may be dispensable for Y. enterocolitica systemic disease and inflammatory resp

72 Establishment of S2 cells as a model for Y. enterocolitica infection provides a versatile tool to

73 e cAMP-CRP regulatory system is required for Y. enterocolitica virulence.

74 ne identified along with inv in a screen for Y. enterocolitica genes that could confer an invasive ph

75 Analysis of the core gene set for Y. enterocolitica revealed that 20.8% of the genes were

76 ucleotide sequence of the 16S rRNA gene from Y. enterocolitica were designed.

77 4, with and without this bend, isolated from Y. enterocolitica were resolved by using chloroquine gel

78 stis KIM D27 LcrV (LcrV(D27)) bind LcrV from Y. enterocolitica O:9 strain W22703 (LcrV(W22703)) or O:

79 We also show that RovA and H-NS from Y. enterocolitica bind to a similar region of the inv pr

80 activity, we have characterized the OGL from Y. enterocolitica, YeOGL, on oligogalacturonides and det

81 direct the secretion of an Npt reporter from Y. enterocolitica, indicating that a universal targeting

82 An aspirate from the axillary mass grew Y. enterocolitica.

83 These results reveal facets of how Y. enterocolitica controls the function of the Ysa TTS s

84 These findings add a new aspect of how Y. enterocolitica effectively evades the host complement

85 However, Y. enterocolitica had no effect on S. Typhimurium uptake

86 Yersinia bercovieri, a recently identified Y. enterocolitica-like species, produces a heat-stable e

87 In Y. enterocolitica, a rovA mutant is attenuated for virul

88 erefore, we investigated the role of ArcB in Y. enterocolitica and E. coli.

89 Mutating all CSC-boxes in Y. enterocolitica of a plasmid bound cspA1/A2 dramatical

90 y, when YspP was constitutively expressed in Y. enterocolitica bv.

91 g mechanism for virulence gene expression in Y. enterocolitica and other enteric pathogens.

92 ositive regulators of psp gene expression in Y. enterocolitica.

93 d the gene for the lipoprotein YlpA found in Y. enterocolitica likely is a pseudogene in Y. pestis.

94 ia coli galE mutant, its primary function in Y. enterocolitica is not in the production of UDP galact

95 Furthermore, in Y. enterocolitica RovM only in the presence of Hfq affec

96 a CSC-box into a plasmid-bound lacZ gene in Y. enterocolitica, the mRNA of this construct was cleave

97 noyl)-l-homoserine lactone (3-oxo-C6-HSL) in Y. enterocolitica and inhibit QS-associated biofilm matu

98 ditional galE homolog has been identified in Y. enterocolitica by homology to the E. coli gene.

99 pholipase YplA, which has been implicated in Y. enterocolitica virulence.

100 tion that a number of RovA-regulated loci in Y. enterocolitica do not have orthologues in Y. pestis a

101 logues in Yersinia pestis plasmid pCD1 or in Y. enterocolitica serotype 0:9 plasmid pYVe227.

102 endogenous chromosomally encoded proteins in Y. enterocolitica revealed discrete complexes correspond

103 FtsH destabilizes PspC in Y. enterocolitica, but coproduction of PspC with its bin

104 the membrane topologies of PspB and PspC in Y. enterocolitica.

105 r to alleviate transcriptional repression in Y. enterocolitica.

106 olog has been demonstrated to have a role in Y. enterocolitica serotype 0:8 O-polysaccharide antigen

107 entified a new positive regulator of rovA in Y. enterocolitica, LeuO.

108 nal response to prolonged secretin stress in Y. enterocolitica.

109 oli, Salmonella, and Yersinia pestis than in Y. enterocolitica.

110 s KIM6+ system is most homologous to that in Y. enterocolitica, showing identities of 84% for YfuA (p

111 le in the modulation of ail transcription in Y. enterocolitica.

112 ulosis while negatively regulated by YmoA in Y. enterocolitica and H-NS in Y. pseudotuberculosis.

113 pseudotuberculosis and Y. pestis and YopP in Y. enterocolitica has been shown to regulate host immune

114 been linked to robust phenotypes, including Y. enterocolitica virulence.

115 le of targeting YopP and that they influence Y. enterocolitica interactions with macrophages.

116 fices to protect against an otherwise lethal Y. enterocolitica challenge.

117 dendritic cells, and a yopP mutant of a live Y. enterocolitica carrier vaccine elicited effective pri

118 eless, the ytxAB genes are conserved in many Y. enterocolitica strains.

119 In adult mice, Y. enterocolitica rapidly disseminated to the spleen and

120 Unlike S. typhimurium flgM mutants, Y. enterocolitica flgM mutants are fully virulent.

121 genes in response to S. typhimurium but not Y. enterocolitica.

122 Subsequently, a yplA-null Y. enterocolitica strain, YEDS10, was constructed and de

123 at the Ysa TTS system impacts the ability of Y. enterocolitica to colonize gastrointestinal tissues.

124 In this study, we tested the ability of Y. enterocolitica to modulate intracellular IL-1alpha-de

125 uction of gut inflammation characteristic of Y. enterocolitica infection.

126 Using a genetic approach, a collection of Y. enterocolitica Ysa TTS mutants was generated by mutag

127 and inflammatory cytokines in the control of Y. enterocolitica infection in IL-6(-/-) mice was undert

128 he role of the Ysps during the life cycle of Y. enterocolitica.

129 ion of YscM1 and YscM2 from the cytoplasm of Y. enterocolitica causes an increase in yop expression,

130 potential for use in the rapid detection of Y. enterocolitica contamination in stored blood units.

131 ors are conserved in Yersinia, divergence of Y. enterocolitica and Y. pseudotuberculosis/Y. pestis du

132 evidence that it is the C-terminal domain of Y. enterocolitica PspC (PspC(CT)) that interacts directl

133 initial examination of the effectiveness of Y. enterocolitica cya and crp mutants to stimulate prote

134 common with the heat-stable enterotoxins of Y. enterocolitica (YST I and YST II), it appears to be a

135 Examination of Y. enterocolitica-infected J774A.1 macrophages revealed

136 The ail gene of Y. enterocolitica is regulated by temperature and growth

137 The pspC gene of Y. enterocolitica was found to be important for normal g

138 gulates potentially novel virulence genes of Y. enterocolitica during infection.

139 oteases in a screen for chromosomal genes of Y. enterocolitica that were exclusively expressed during

140 re unable to block the type III injection of Y. enterocolitica strains, expression of lcrV(W22703) or

141 tica infections, defined as the isolation of Y. enterocolitica or unspeciated Yersinia from a human c

142 In this study, the psp locus of Y. enterocolitica was characterized further.

143 In addition, motility of Y. enterocolitica is regulated by temperature.

144 s indicate that an inv yadA double mutant of Y. enterocolitica is avirulent while an inv yadA mutant

145 through mice infected with a yenI mutant of Y. enterocolitica that cannot synthesize AHLs.

146 we screened transposon insertion mutants of Y. enterocolitica W22703 for defects in type III secreti

147 samples were spiked with various numbers of Y. enterocolitica cells, and total chromosomal DNA was e

148 tinct from the flagella secretion pathway of Y. enterocolitica.

149 mporally dynamic gene expression patterns of Y. enterocolitica biovar 1B through the course of an in

150 it secretes (Yops), prevents phagocytosis of Y. enterocolitica and is required for disease processes

151 he N-acylhomoserine lactone (AHL) profile of Y. enterocolitica.

152 ctly and specifically to the inv promoter of Y. enterocolitica.

153 to change the incompatibility properties of Y. enterocolitica serotype 0:8 plasmids from those of Y.

154 nv, the gene encoding the invasin protein of Y. enterocolitica, hreP is located in a cluster of flage

155 in this study we show that PspB and PspC of Y. enterocolitica are dual function proteins, acting bot

156 YadA, which is the primary C4BP receptor of Y. enterocolitica.

157 little overlap between the RovA regulons of Y. enterocolitica and Y. pestis despite the fact that Ro

158 single factor mediating serum resistance of Y. enterocolitica, presumably by binding C4b binding pro

159 Sequence analysis of the JB580v strain of Y. enterocolitica shows that, due to a premature stop co

160 A ure mutant strain of Y. enterocolitica was constructed which was hypersensiti

161 tigate a diverse collection of 94 strains of Y. enterocolitica consisting of 35 human, 35 pig, 15 she

162 Nonmotile strains of Y. enterocolitica were less invasive than motile strains

163 ase for the existence of three subspecies of Y. enterocolitica.

164 is apparently essential for the survival of Y. enterocolitica during infection.

165 ed as being required for in vivo survival of Y. enterocolitica.

166 pendent and distantly related TTS systems of Y. enterocolitica recognize protein substrates by a simi

167 tein (invasin(pstb)) was compared to that of Y. enterocolitica invasin (invasin(ent)), which lacks th

168 colitica serotype 0:8 plasmids from those of Y. enterocolitica serotype 0:9 and Y. pestis LCR plasmid

169 uctural subunits is most similar to those of Y. enterocolitica urease.

170 ave opened with the discovery of the Ysps of Y. enterocolitica Biovar 1B, which are translocated into

171 a 17kDa cell-surface protein that confers on Y. enterocolitica resistance to serum killing and the ab

172 escribed here compare oral S. typhimurium or Y. enterocolitica infection in stromelysin-1 (MMP-3)-def

173 es of 7-day-old and adult mice to orogastric Y. enterocolitica infection were assessed in 50% lethal

174 rium is primarily an intracellular pathogen, Y. enterocolitica survives primarily extracellularly.

175 n the pathogenic Yersiniae (Yersinia pestis, Y. enterocolitica, and Y. pseudotuberculosis).

176 al for protection of neonates during primary Y. enterocolitica infection.

177 To determine how temperature regulates Y. enterocolitica motility, we have been dissecting the

178 To subvert the host's immune response, Y. enterocolitica uses a type III secretion system consi

179 tructed for four hre genes and the resulting Y. enterocolitica mutants were tested in the mouse model

180 common to three pathogenic Yersinia species: Y. enterocolitica, Y. pseudotuberculosis, and Y. pestis.

181 plasmids are from a common ancestor but that Y. enterocolitica serotype 0:8 plasmid replicons may hav

182 ults, biochemical evidence demonstrated that Y. enterocolitica and M. morganii ureases were activated

183 We show here that Y. enterocolitica is capable of infecting S2 cells and r

184 Overall, these studies support the idea that Y. enterocolitica promotes the development of highly inf

185 Previously, we reported that Y. enterocolitica PspB functions to positively control t

186 Further analysis revealed that Y. enterocolitica does not cluster according to source (

187 Here we show that Y. enterocolitica polymerizes a 6-kDa protein of the sec

188 ding model of factor H to YadA and show that Y. enterocolitica YadA recruits C3b and iC3b directly, w

189 In this study, we showed that Y. enterocolitica serotype O8 survives buffered acidic c

190 These data suggest that Y. enterocolitica inhibits intracellular pre-IL-1alpha s

191 Together these findings suggest that Y. enterocolitica produces a phospholipase A which has a

192 The Y. enterocolitica ClpB homologue is 30 to 40% identical

193 The Y. enterocolitica clpP gene complemented the clpP mutant

194 The Y. enterocolitica fliA gene, encoding the flagellar-spec

195 The Y. enterocolitica psp locus is made up of two divergentl

196 The Y. enterocolitica pspG gene was identified because its p

197 Comparisons between the Y. enterocolitica sif genes and the previously identifie

198 ogene, which occurs as an intact gene in the Y. enterocolitica and Y. pseudotuberculosis-derived anal

199 nd ytxAB genes are not closely linked in the Y. enterocolitica chromosome, and whereas ytxR is presen

200 t H-NS and RovA bind is not conserved in the Y. enterocolitica promoter.

201 otype, Y. pseudotuberculosis homologs of the Y. enterocolitica ail and the Y. pestis psa loci were id

202 responsible for the unique properties of the Y. enterocolitica and M. morganii ureases since the L. f

203 The in situ structures of the Y. enterocolitica and S. flexneri injectisomes had simil

204 Identification of the Y. enterocolitica fliA and flgM genes was accomplished b

205 1122 recombined with a close relative of the Y. enterocolitica phage phiYeO3-12 to yield progeny phag

206 refore, PspG is the missing component of the Y. enterocolitica Psp regulon that was previously predic

207 We also compared induction of the Y. enterocolitica Psp, RpoE, and Cpx responses.

208 al regulator that controls expression of the Y. enterocolitica ytxAB genes.

209 These data suggest that the Y. enterocolitica pYV plasmid may undergo a conformation

210 nt protein reporters, we determined that the Y. enterocolitica rovA (rovA(Yent)) promoter is weaker t

211 Here, we present evidence that the Y. enterocolitica virulence plasmid, pYV, undergoes a co

212 Even though Y. enterocolitica induces a robust inflammatory response

213 f the bacteria to colonize neonatal tissues; Y. enterocolitica was readily detectable in the intestin

214 est that the Ysa and Ysc TTSSs contribute to Y. enterocolitica virulence by exporting both unique and

215 expressed in mucosal tissues, contributes to Y. enterocolitica pathogenicity by undermining protectiv

216 ll responses elicited in neonates exposed to Y. enterocolitica were associated with long-term protect

217 d obtained from mouse antiserum generated to Y. enterocolitica.

218 nfection, C57BL/6 mice are more resistant to Y. enterocolitica than are BALB/c mice.

219 eful model for studying the host response to Y. enterocolitica infection.

220 as significantly up-regulated in response to Y. enterocolitica infection.

221 r IFN-gamma that are produced in response to Y. enterocolitica.

222 f IL-1 alpha in mice infected with wild-type Y. enterocolitica results in significantly decreased int

223 ly 10-fold higher than that of the wild-type Y. enterocolitica, and there are significant inflammator

224 a yopP-deficient strain than with wild-type Y. enterocolitica, whereas only modest increases occurre

225 es compared to those infected with wild-type Y. enterocolitica.

226 eristics previously described following i.v. Y. enterocolitica infection.

227 l methods for the detection of ETEC, Vibrio, Y. enterocolitica, and P. shigelloides in stool specimen

228 obtained using two different high-virulence Y. enterocolitica strains.

229 of neonatal mice with low doses of virulent Y. enterocolitica leads to vigorous intestinal and syste

230 f a spontaneously arising pathogenic Ab with Y. enterocolitica.

231 Infection of eukaryotic cells with Y. enterocolitica strains expressing a Ysp-CyaA chimeric

232 t was prevented when mice were infected with Y. enterocolitica lacking YopP or YopH, two critical eff

233 rvival of MMP-3-deficient mice infected with Y. enterocolitica when compared with littermate controls

234 of intestinal lymphatic tissue infected with Y. enterocolitica.

235 nteric lymph nodes after oral infection with Y. enterocolitica.

236 dose (LD(50)) following oral infection with Y. enterocolitica.

237 After i.p. inoculation with Y. enterocolitica, fibrinogen-deficient mice display imp

238 llowing either i.p. or i.v. inoculation with Y. enterocolitica.

239 es AHLs to mimic a constant interaction with Y. enterocolitica.

240 patches of mice infected orogastrically with Y. enterocolitica serotype O:8 compared with noninfected

241 A, OmpC, and OmpF confirming reactivity with Y. enterocolitica.

242 The role of microbial Ags was tested with Y. enterocolitica proteins.