ライフサイエンスコーパス: Y. pseudotuberculosis

1 Y. pseudotuberculosis binding to cells caused robust rec

2 Y. pseudotuberculosis expressing wild-type YpkA, but not

3 Y. pseudotuberculosis phoP mutants died at a low rate in

4 Y. pseudotuberculosis phoP mutants were unable to replic

5 Y. pseudotuberculosis strains also varied greatly in the

6 Y. pseudotuberculosis translocated to organs such as the

7 signature-tagged mutagenesis, we isolated 13 Y. pseudotuberculosis mutants that failed to survive in

8 Five of 18 Y. pseudotuberculosis strains, encompassing seven seroty

9 a-like strains, 22 Y. pestis strains, and 21 Y. pseudotuberculosis strains from 130,574 clinical and

10 A Y. pseudotuberculosis phoP mutant was 100-fold less viru

11 ly to promote cytoskeletal disruption, and a Y. pseudotuberculosis mutant lacking YpkA GDI activity s

12 ar, a translocation defect was observed in a Y. pseudotuberculosis strain that expressed an uptake-de

13 However, in a Y. pseudotuberculosis ypsI-negative background, YtbI app

14 ipid A, and an attenuated strain producing a Y. pseudotuberculosis-like hexa-acylated lipid A.

15 In addition, Y. pseudotuberculosis mutants expressing YopM proteins u

16 ed how host cell sterol composition affected Y. pseudotuberculosis uptake.

17 Decreases in NK cells after Y. pseudotuberculosis infection did not correlate with Y

18 Although Y. pseudotuberculosis makes biofilms in other environmen

19 stis isolates and its evolutionary ancestor, Y. pseudotuberculosis.

20 an intact gene in the Y. enterocolitica and Y. pseudotuberculosis-derived analogues, was found in pC

21 iption of inv in Yersinia enterocolitica and Y. pseudotuberculosis.

22 inv regulation between Y. enterocolitica and Y. pseudotuberculosis.

23 ersinia, divergence of Y. enterocolitica and Y. pseudotuberculosis/Y. pestis during evolution has res

24 iae (Yersinia pestis, Y. enterocolitica, and Y. pseudotuberculosis).

25 lla pneumoniae, Yersinia enterocolitica, and Y. pseudotuberculosis.

26 . pestis, the agent of plague in humans, and Y. pseudotuberculosis, the related enteric pathogen, del

27 lly, when antiphagocytosis is incomplete and Y. pseudotuberculosis is internalized by macrophages, th

28 present on the chromosomes of Y. pestis and Y. pseudotuberculosis and that this secretion system is

29 However, both Y. pestis and Y. pseudotuberculosis form biofilms that adhere to the e

30 Studies in Y. pestis and Y. pseudotuberculosis have shown that YopM suppresses in

31 te in macrophages is common to Y. pestis and Y. pseudotuberculosis or is a unique phenotype of Y. pes

32 A number of Y. pestis and Y. pseudotuberculosis strains of different biovars and s

33 macrophages compared to other Y. pestis and Y. pseudotuberculosis strains tested.

34 logous sequences from numerous Y. pestis and Y. pseudotuberculosis strains, we determined that these

35 ars, serotypes, and strains of Y. pestis and Y. pseudotuberculosis that may relate to the evolution o

36 We compared the ability of Y. pestis and Y. pseudotuberculosis to infect the rat flea Xenopsylla

37 n cleavage is specific for the Y. pestis and Y. pseudotuberculosis YapE orthologues but is not conser

38 hat biofilms produced by Yersinia pestis and Y. pseudotuberculosis, which bind the C. elegans surface

39 in-specific manner and only in Y. pestis and Y. pseudotuberculosis.

40 in macrophages is conserved in Y. pestis and Y. pseudotuberculosis.

41 acquisition of this island by Y. pestis and Y. pseudotuberculosis.

42 ops do not completely block phagocytosis and Y. pseudotuberculosis can replicate in macrophages, it i

43 m and on wild-type Y. pseudotuberculosis and Y. pseudotuberculosis QS mutants.

44 Six sRNAs are Y. pseudotuberculosis specific and are absent from the g

45 ght junctions) to the apical compartment, as Y. pseudotuberculosis cells lacking the inv gene were un

46 est that it is possible to use an attenuated Y. pseudotuberculosis strain delivering the LcrV antigen

47 e expression of many sRNAs conserved between Y. pseudotuberculosis and Y. pestis differs in both timi

48 perons had high levels of divergence between Y. pseudotuberculosis strains.

49 This suggests that both Y. pseudotuberculosis and Y. pestis produce an oligosacc

50 eading to increased systemic colonization by Y. pseudotuberculosis and potentially enhancing adaptive

51 effects of spleen and liver colonization by Y. pseudotuberculosis on yop mutants in the intestines.

52 that three virulence determinants encoded by Y. pseudotuberculosis manipulate the Rho GTPase RhoG.

53 ork also indicates that biofilm formation by Y. pseudotuberculosis on C. elegans is an interactive pr

54 ty regulator, FlhDC, in biofilm formation by Y. pseudotuberculosis on C. elegans.

55 used to show efficient RhoG inactivation by Y. pseudotuberculosis YopE, a potent Rho GTPase activati

56 monolayers became susceptible to invasion by Y. pseudotuberculosis.

57 We show that the cell death mediated by Y. pseudotuberculosis is apoptosis.

58 Here we show that Hfq is required by Y. pseudotuberculosis to cause mortality in an intragast

59 he global set of sRNAs expressed in vitro by Y. pseudotuberculosis.

60 itment of both fH and C4BP by Ail may confer Y. pseudotuberculosis with the ability to resist all pat

61 In contrast, Y. pseudotuberculosis strains which did not contain IS10

62 As the YopD(DeltaTM) Y. pseudotuberculosis mutant formed somewhat smaller por

63 val of pYV(+) but not pYV-cured or DeltayopB Y. pseudotuberculosis within phagosomes: only a small fr

64 l assay (type III secretion system dependent Y. pseudotuberculosis cytotoxicity assay).

65 hogenic Yersinia species: Y. enterocolitica, Y. pseudotuberculosis, and Y. pestis.

66 ust immune response against enteropathogenic Y. pseudotuberculosis by promoting Th17-like responses i

67 , the GAP function of YopE was important for Y. pseudotuberculosis pathogenesis in a mouse infection

68 replication in macrophages are important for Y. pseudotuberculosis pathogenesis.

69 Here the importance of PhoP for Y. pseudotuberculosis pathogenesis was investigated.

70 Therefore, YopJ is required for Y. pseudotuberculosis to downregulate MAP kinases and in

71 An increased risk for Y. pseudotuberculosis infection was specifically related

72 E and PNPase can be readily copurified from Y. pseudotuberculosis cell extracts, suggests that these

73 s is similar to recently published data from Y. pseudotuberculosis, revealing a potentially conserved

74 ear which of these phenotypes descended from Y. pseudotuberculosis and which were acquired independen

75 estis acquired since the the divergence from Y. pseudotuberculosis.

76 Yersinia pestis evolved from Y. pseudotuberculosis to become the causative agent of b

77 PCP1 during the divergence of Y. pestis from Y. pseudotuberculosis, and are the first evidence of a n

78 cement of the pseudogene with the functional Y. pseudotuberculosis rcsA allele strongly represses bio

79 he contribution of this region by generating Y. pseudotuberculosis yopD(Delta150-170) and yopD(Delta2

80 During aerobic growth on glucose, Y. pseudotuberculosis reveals an unusual flux distributi

81 IV(A) modified with C10 or C12 acyl groups, Y. pseudotuberculosis contained the same forms as part o

82 two Yersinia species pathogenic for humans (Y. pseudotuberculosis and Y. enterocolitica).

83 In Y. pseudotuberculosis, rovA transcription is controlled

84 ith a hierarchical quorum-sensing cascade in Y. pseudotuberculosis that is involved in the regulation

85 YPTB3286), we show that yapK is conserved in Y. pseudotuberculosis, while yapJ is unique to Y. pestis

86 evealing potential virulence determinants in Y. pseudotuberculosis that are regulated in a posttransc

87 regulator of biofilms that is functional in Y. pseudotuberculosis, is a pseudogene in Y. pestis.

88 xpression of virulence-relevant functions in Y. pseudotuberculosis and reprogramming of its metabolis

89 Disruption of the ail gene in Y. pseudotuberculosis resulted in loss of C4BP binding.

90 ver, the deletion of the homologous genes in Y. pseudotuberculosis does not seem to impact the virule

91 g yapK and yapJ to three homologous genes in Y. pseudotuberculosis IP32953 (YPTB0365, YPTB3285, and Y

92 mophagocyte in this disease model but not in Y. pseudotuberculosis-infected mice.

93 tion at 37 degrees C in Y. pestis but not in Y. pseudotuberculosis.

94 ted by YmoA in Y. enterocolitica and H-NS in Y. pseudotuberculosis.

95 Conversely, deletion of the urease operon in Y. pseudotuberculosis rendered it nontoxic.

96 mologous to those of the cognate plasmids in Y. pseudotuberculosis and Y. enterocolitica, but their l

97 stem (TTSS) found in Y. pestis is present in Y. pseudotuberculosis and whether this system is importa

98 he hms genes; identical genes are present in Y. pseudotuberculosis.

99 okine CCL28 was increased by loss of rfaH in Y. pseudotuberculosis but not in Y. pestis.

100 Deletion of multiple sRNAs in Y. pseudotuberculosis leads to attenuation of the pathog

101 Because a key step in Y. pseudotuberculosis internalization is interaction of

102 Primer extension analyses indicate that, in Y. pseudotuberculosis, the transcription of the psaE and

103 with these results, IL-10 is undetectable in Y. pseudotuberculosis-infected mouse tissues until advan

104 Virulence in Y. pseudotuberculosis requires the plasmid-encoded Ysc t

105 I secretion system effector known as YopJ in Y. pseudotuberculosis and Y. pestis and YopP in Y. enter

106 ng enzymatic activities of YopE and YopT, in Y. pseudotuberculosis-infected macrophages.

107 er the T3SS can be utilized by intracellular Y. pseudotuberculosis to translocate Yops.

108 t was protective against lethal intragastric Y. pseudotuberculosis challenge.

109 Last, Y. pseudotuberculosis did not require Ivy to counter lys

110 ted the macrophage cytokine response to live Y. pseudotuberculosis and analyzed the susceptibility of

111 attempt to reduce binding to luminal mucus, Y. pseudotuberculosis yadA and inv yadA strains were ana

112 strate the misregulation of RhoG by multiple Y. pseudotuberculosis virulence determinants.

113 Mutant Y. pseudotuberculosis that do not make any Yop proteins

114 acrophages infected with wild-type or mutant Y. pseudotuberculosis strains.

115 Phagosomes containing phoP mutant Y. pseudotuberculosis acquired cathepsin D at a higher r

116 phagosomes containing phoP(+) or phoP mutant Y. pseudotuberculosis was studied by using immunofluores

117 pYV-cured, pYV(+) wild-type, and yop mutant Y. pseudotuberculosis strains were allowed to infect bon

118 intravenously with wild-type or yopM mutant Y. pseudotuberculosis strains.

119 detected, indicating that as many as 13% of Y. pseudotuberculosis genes no longer function in Y. pes

120 The ability of Y. pseudotuberculosis to promote apoptosis of macrophage

121 ars intimately connected with the ability of Y. pseudotuberculosis to successfully establish replicat

122 Transcriptomic analysis of Y. pseudotuberculosis revealed that genes located in bot

123 ranasal inoculation with as few as 18 CFU of Y. pseudotuberculosis caused a lethal lung infection in

124 nds inhibited T3SS-dependent cytotoxicity of Y. pseudotuberculosis toward macrophages in vitro.

125 The sterol structure dependence of Y. pseudotuberculosis internalization differed from that

126 th in the intestine delayed dissemination of Y. pseudotuberculosis.

127 id containing a 6.7-kb KpnI-ClaI fragment of Y. pseudotuberculosis encompassing the psa locus was suf

128 ia pestis and pesticin-sensitive isolates of Y. pseudotuberculosis possess a common 34 kbp DNA region

129 In an orogastric model of Y. pseudotuberculosis infection, a Delta yopM mutant was

130 In a murine model of Y. pseudotuberculosis pneumonia, two compounds significa

131 Furthermore, the Deltahfq mutant of Y. pseudotuberculosis is hypermotile and displays increa

132 ica is avirulent while an inv yadA mutant of Y. pseudotuberculosis is hypervirulent.

133 ersinia, we constructed DeltarfaH mutants of Y. pseudotuberculosis IP32953 and Y. pestis KIM6+.

134 New constructs of the inv yadA mutants of Y. pseudotuberculosis were made and tested in mice.

135 es and prepared lpp gene deletion mutants of Y. pseudotuberculosis YPIII, Y. pestis KIM/D27 (pigmenta

136 characterized a glycosyl hydrolase (NghA) of Y. pseudotuberculosis that cleaved beta-linked N-acetylg

137 regions were specific to a limited number of Y. pseudotuberculosis strains, including the high pathog

138 bacterium and host following phagocytosis of Y. pseudotuberculosis suggests new roles for the T3SS in

139 T3SS expression levels among a population of Y. pseudotuberculosis cells following the removal of Ca(

140 Random screening of Y. pseudotuberculosis transposon insertion mutants with

141 , we report the complete genomic sequence of Y. pseudotuberculosis IP32953 and its use for detailed g

142 th either the IP32953 or the 32777 strain of Y. pseudotuberculosis, demonstrating that the phenotype

143 of 22 strains of Y. pestis and 10 strains of Y. pseudotuberculosis of diverse origin.

144 atalytically inactive yopJ mutant strains of Y. pseudotuberculosis was developed to further investiga

145 absent or highly divergent in all strains of Y. pseudotuberculosis.

146 molecular marker that identifies a subset of Y. pseudotuberculosis isolates that have a particularly

147 ly, our results suggest that the Ysc T3SS of Y. pseudotuberculosis can function within macrophage pha

148 Through the use of Y. pseudotuberculosis mutants, we show that the inhibito

149 fq plays a critical role in the virulence of Y. pseudotuberculosis by participating in the regulation

150 e in allowing adhesion of M. nematophilum or Y. pseudotuberculosis biofilm to wild type C. elegans.

151 Though the closely related enteric pathogen Y. pseudotuberculosis uses quorum sensing system to regu

152 y evolved from the gastrointestinal pathogen Y. pseudotuberculosis; however, it is not known at what

153 ia species pathogenic for humans (Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica) export and

154 fy the genes responsible for this phenotype, Y. pseudotuberculosis homologs of the Y. enterocolitica

155 phoP(+) Y. pseudotuberculosis strains initiated replication in m

156 tants, suggesting that YopE and YopH protect Y. pseudotuberculosis from these defenses in BALB/c mice

157 e macrophage response to internalized pYV(+) Y. pseudotuberculosis.

158 f macrophages harboring intracellular pYV(+) Y. pseudotuberculosis with chloramphenicol reduced apopt

159 f macrophages infected with wild-type pYV(+) Y. pseudotuberculosis died of apoptosis after 20 h.

160 Y. pestis and the closely related Y. pseudotuberculosis also block the feeding of Caenorha

161 of plague, from the closely related species Y. pseudotuberculosis.

162 Upon TLR-2 stimulation, Y. pseudotuberculosis-infected monocytes activated caspa

163 In this article, we demonstrate that Y. pseudotuberculosis Ail from strains PB1, 2812/79, and

164 These results indicate that Y. pseudotuberculosis adhesive factors control the site

165 These results indicate that Y. pseudotuberculosis Ail recruits C4BP in a functional

166 Fluorescence localization indicated that Y. pseudotuberculosis selectively associated with monola

167 o Yops have some redundant functions or that Y. pseudotuberculosis employs multiple strategies for co

168 Here we report that Y. pseudotuberculosis cells with reduced RNase E activit

169 erial cell binding and entry mediated by the Y. pseudotuberculosis invasin protein (invasin(pstb)) wa

170 To determine the Y. pseudotuberculosis factors important for growth durin

171 These changes from the Y. pseudotuberculosis progenitor included loss of insect

172 Mutations in the Y. pseudotuberculosis ail and psa loci were constructed

173 ort a model in which a genetic change in the Y. pseudotuberculosis progenitor of Y. pestis extended i

174 he C-terminal 192-residue superdomain of the Y. pseudotuberculosis invasin is necessary and sufficien

175 orm from the cell by the introduction of the Y. pseudotuberculosis YopE RhoGAP protein could be bypas

176 ed sensitivity to host defense peptides, the Y. pseudotuberculosis DeltarfaH strain was not attenuate

177 Only one of six strains tested, the Y. pseudotuberculosis YPIII(p(-)) strain, was defective

178 ovA (rovA(Yent)) promoter is weaker than the Y. pseudotuberculosis promoter.

179 luding those phylogenetically closest to the Y. pseudotuberculosis progenitor, contain a mutated ureD

180 and RovA bind with a higher affinity to the Y. pseudotuberculosis/Y. pestis rovA (rovA(Ypstb/Ypestis

181 Therefore, Y. pseudotuberculosis subverts intestinal barrier functi

182 Three Y. pseudotuberculosis factors, previously identified by

183 estis strains (EV766 and KIM10(+)) and three Y. pseudotuberculosis strains (IP2790c, IP2515c, and IP2

184 ant produced similar levels of antibodies to Y. pseudotuberculosis antigens and were equally resistan

185 rtant mechanism by which YopM contributes to Y. pseudotuberculosis virulence.

186 monstrate that after a transient exposure to Y. pseudotuberculosis, T and B cells are impaired in the

187 optotic response of TLR2(-/-) macrophages to Y. pseudotuberculosis infection is equivalent to that of

188 ence and absence of biofilm and on wild-type Y. pseudotuberculosis and Y. pseudotuberculosis QS mutan

189 ine macrophages were infected with wild-type Y. pseudotuberculosis but not with an isogenic ysc mutan

190 spleens showed that infection with wild-type Y. pseudotuberculosis caused an influx of neutrophils, w

191 Infection with wild-type Y. pseudotuberculosis caused significantly more inflamma

192 To determine how wild-type Y. pseudotuberculosis hinders yop mutant survival, yop m

193 that isolated specific features of wild-type Y. pseudotuberculosis infection.

194 Wild-type Y. pseudotuberculosis kills approximately 70% of infecte

195 Over the course of 7 days, wild-type Y. pseudotuberculosis replicated to nearly 1 x 10(8) CFU

196 Infection of C. elegans with the wild-type Y. pseudotuberculosis resulted in the differential regul

197 ail to persist in competition with wild-type Y. pseudotuberculosis, indicating that these two infecti

198 In competition infections with wild-type Y. pseudotuberculosis, the presence of wild-type bacteri

199 epithelial cells as efficiently as wild-type Y. pseudotuberculosis.

200 In addition, using Y. pseudotuberculosis strains marked with signature tags

201 This possibility was investigated here using Y. pseudotuberculosis strains that express YopJ or YopH

202 ld be correlated with the presence of viable Y. pseudotuberculosis in macrophages.

203 is no consensus in the literature on whether Y. pseudotuberculosis shares this property.

204 e compared the sterol dependence of wildtype Y. pseudotuberculosis internalization with that of Delta

205 efold in TLR4(-/-) macrophages infected with Y. pseudotuberculosis, while the apoptotic response of T

206 ions in human epithelial cells infected with Y. pseudotuberculosis.

207 poptosis (YopJ) in macrophages infected with Y. pseudotuberculosis.

208 In summary, infection with Y. pseudotuberculosis in intestinal and systemic sites i

209 the response) during primary infection with Y. pseudotuberculosis, as shown by flow cytometry tetram

210 Results of infections with Y. pseudotuberculosis expressing catalytically inactive

211 Finally, competition infections with Y. pseudotuberculosis mutants with various abilities to

212 Furthermore, this lung infection model with Y. pseudotuberculosis can be used to test potential ther

213 ma) in serum samples of mice vaccinated with Y. pseudotuberculosis.

214 killing of macrophages infected ex vivo with Y. pseudotuberculosis, suggesting a mechanism by which t

215 y, HeLa cells infected with wild-type or Yop-Y. pseudotuberculosis strains were assayed for interleuk