ライフサイエンスコーパス: abortive initiation

1 racking and arrest in a process analogous to abortive initiation.

2 ape and consequently enhancing the extent of abortive initiation.

3 relates with a markedly reduced frequency of abortive initiation.

4 initiation shows characteristics of in vitro abortive initiation: Abortive initiation increases upon

5 Abortive initiation and promoter escape are two principa

6 transcription reactions to better understand abortive initiation and promoter escape in vivo.

7 ing-laser excitation, we were able to detect abortive initiation and promoter escape within single im

8 titative initiation parameters that describe abortive initiation and promoter escape; these parameter

9 ex at the point where steric clash initiates abortive initiation and sigma(A) dissociation.

10 ranscript must displace the loop, leading to abortive initiation and ultimately to sigma release.

11 to the open initiation complex, frequency of abortive initiation, and pausing during transcript elong

12 of the polymerase complex, as determined by abortive initiation, and promoter clearance and/or reini

13 leading edge and DNA downstream of RNAP upon abortive initiation, and we observe large decreases in d

14 Abortive initiation assays at 37 degrees C indicate that

15 Abortive initiation assays confirm that ExsA enhances th

16 Abortive initiation assays have been used to quantify th

17 activation function of the mutant activator, abortive initiation assays were performed, using purifie

18 tro transcription, DNase I footprinting, and abortive initiation assays were used to characterize tra

19 Permanganate probing and abortive initiation assays were used to investigate the

20 Abortive initiation assays were used to show that this a

21 KMnO(4) footprinting, abortive initiation assays, and the formation of the spe

22 magnitude greater than the value reported in abortive initiation assays.

23 ively and quantitative different patterns of abortive initiation at the same start site.

24 AsiA on open promoter complex formation and abortive initiation at two -10/-35 type promoters and tw

25 Thus, the level of abortive initiation by RNA polymerase II is at least par

26 a transcript is extended to +2 and +3 in an abortive initiation complex.

27 f the transcript caused similar increases in abortive initiation for transcription of bubble template

28 ffect on open promoter complex formation and abortive initiation from extended -10 promoters, which l

29 s, and both a downstream shift and increased abortive initiation in reconstituted transcription assay

30 tion yields of a short AAUU transcript in an abortive initiation in vitro transcription assay.

31 acteristics of in vitro abortive initiation: Abortive initiation increases upon stabilizing interacti

32 ibutions and single-molecule time traces for abortive initiation indicates that, at a consensus promo

33 -molecule DNA nanomanipulation, we show that abortive initiation involves DNA "scrunching"--in which

34 Ase I footprinting, KMnO(4)-sensitivity, and abortive initiation kinetic analysis.

35 Abortive initiation may be viewed as promoter proofreadi

36 It has not been known whether abortive initiation occurs in vivo.

37 Dinucleotide-primed abortive initiation of basal and activated T4 late trans

38 Substitution of 5-bromo-UTP for UTP reduced abortive initiation on bubble templates, consistent with

39 deletion had no effect on promoter binding, abortive initiation or promoter escape, TFIIS-stimulated

40 may interfere with RNA exit, which leads to abortive initiation or promoter escape.

41 that nearly all pol III molecules escape the abortive initiation phase of transcription without signi

42 find that, on average, only three rounds of abortive initiation precede the formation of each elonga

43 resence of phosphate-free P protein produced abortive initiation products but no full-length transcri

44 purification away from excess nucleotide and abortive initiation products so that the purified comple

45 n initiation and elongation; however, longer abortive initiation products were produced in the presen

46 nscription but yielded an altered pattern of abortive initiation products, indicating that the R78C d

47 anscribed sequence (ITS) of N25 lengthen the abortive initiation program, resulting in the release of

48 a'omegasigma(70)), we compare productive and abortive initiation rates, short RNA distributions, and

49 Kinetic analyses of productive and abortive initiation showed that CAP acted both to stabil

50 In addition, we show that in vivo abortive initiation shows characteristics of in vitro ab

51 nly after carrying out an obligate series of abortive initiation steps.

52 es in HeLa extracts gave very high levels of abortive initiation, suggesting that inability to reanne

53 karyotic RNA polymerase (RNAP) can engage in abortive initiation-the synthesis and release of short (

54 Polymerase structure is permissive for abortive initiation, thereby setting a lower limit on po

55 ity, and facilitation of the transition from abortive initiation to productive elongation.

56 he network must occur in the transition from abortive initiation to promoter escape.

57 emplates, consistent with models attributing abortive initiation to the accumulation of strain in the