ライフサイエンスコーパス: abscessus

1 rium avium complex (MAC) and 69 (36%) for M. abscessus.

2 bserved in M. marinum and the smallest in M. abscessus.

3 of a series of indolecarboxamides against M. abscessus.

4 ycobacteria including M. tuberculosis and M. abscessus.

5 topic shifts from incorporation of 15N in M. abscessus.

6 enum varied from those of M. chelonae and M. abscessus.

7 solates of M. chelonae and 25 isolates of M. abscessus.

8 t distinguish Mycobacterium chelonae from M. abscessus.

9 ne sequencing to identify M. chelonae and M. abscessus.

10 tiate M. immunogenum from M. chelonae and M. abscessus.

11 ting that this clone is a subgroup within M. abscessus.

12 erial species Mycobacterium fortuitum and M. abscessus.

13 The isolates were later identified as M. abscessus.

14 ers Mycobacterium smegmatis or Mycobacterium abscessus.

15 occur during chronic lung infection with M. abscessus.

16 20% of U.S. isolates of M. abscessus subsp. abscessus.

17 erm(41) genotype is a useful adjunct for M. abscessus.

18 table to test antibiotic activity against M. abscessus.

19 showed promising in vivo activity against M. abscessus.

20 which we propose the name "Para-streptomyces abscessus."

21 l isolates identified 2 clonal strains of M. abscessus; 1 clone was isolated from water sources at a

22 acterized clinical isolates comprising 29 M. abscessus, 15 M. massiliense, and 2 M. bolletii isolates

23 terium avium complex (72%) and Mycobacterium abscessus (16%) were the most common species.

24 or rapidly growing mycobacteria (98% were M. abscessus), 78% (29 of 37) for M. kansasii, and 26% (9 o

25 Lung infections with Mycobacterium abscessus, a species of multidrug-resistant nontuberculo

26 iduals with cystic fibrosis (CF), in whom M. abscessus accelerates inflammatory lung damage, leading

27 alues for 81 isolates of M. abscessus subsp. abscessus and 12 isolates of M. abscessus subsp. massili

28 identified 41 Mycobacterium abscessus subsp. abscessus and 13 M. abscessus subsp. massiliense isolate

29 A total of 82 isolates (58 M. abscessus and 24 M. chelonae isolates) were tested blind

30 hods, 46 of the isolates were found to be M. abscessus and 29 were identified as M. chelonae.

31 erm(41) sequencing of 285 M. abscessus and 45 M. chelonae isolates was compared to 14

32 l sequencing) is required for division of M. abscessus and closely related species.

33 of two clonal groups for M. abscessus subsp. abscessus and five clonal groups for M. abscesssus subsp

34 reviously identified as being M. chelonae/M. abscessus and identified M. massiliense from isolates fr

35 g of M. fortuitum against clarithromycin; M. abscessus and M. chelonae against the aminoglycosides; a

36 ld be able to easily identify isolates of M. abscessus and M. chelonae.

37 may be helpful for distinguishing between M. abscessus and M. chelonae.

38 enome, regions that discriminated between M. abscessus and M. massiliense were identified through arr

39 d three pairs of closely related strains: M. abscessus and M. massiliense, M. mucogenicum and M. phoc

40 Mycobacterium abscessus and Mycobacterium chelonae are two closely rel

41 rly for distinguishing between Mycobacterium abscessus and Mycobacterium chelonae.

42 olates were typed as M. abscessus subspecies abscessus and were clonally related within each patient.

43 oscopes and endoscopic cleaning machines (M. abscessus) and contaminated hospital water supplies (M.

44 tes of N. cyriacigeorgica, N. asteroides, N. abscessus, and N. otitidiscaviarum were susceptible to t

45 bmitted as M. chelonae were identified as M. abscessus, and one isolate submitted as M. abscessus was

46 cterium fortuitum group, three Mycobacterium abscessus, and three Mycobacterium chelonae isolates) we

47 rium fortuitum group, three of Mycobacterium abscessus, and three of Mycobacterium chelonae) were tes

48 bacterial infections caused by Mycobacterium abscessus are responsible for a range of disease manifes

49 metic mean = 1.5% of hsp65 sequences) and M. abscessus (arithmetic mean = 0.006% of hsp65 sequences).

50 tigated a biphasic outbreak of Mycobacterium abscessus at a tertiary care hospital.

51 Based on the M. abscessus ATCC 19977(T) genome, regions that discriminat

52 We report the first series of Mycobacterium abscessus bacteremia after cytokine-induced killer cell

53 tibility breakpoints for M. abscessus subsp. abscessus be changed from </=2 to </=4 mug/ml and that i

54 ly effective at preventing infection with M. abscessus because it is a ubiquitous environmental sapro

55 ucible macrolide resistance in Mycobacterium abscessus but not Mycobacterium chelonae.

56 stinguishable from Mycobacterium chelonae/M. abscessus by partial 16S rRNA gene sequencing.

57 s demonstrates that the inability to type M. abscessus by PFGE is associated with a single clone of o

58 of using thiourea-containing buffer with M. abscessus by studying 69 isolates not previously typeabl

59 resistance in Mycobacterium abscessus subsp. abscessus, calling into question the usefulness of macro

60 M. abscessus can transition between a noninvasive, biofilm-

61 ses (57%; odds ratio = 0.7, P < 0.05) and M. abscessus cases (51%; odds ratio = 0.5, P < 0.01) than i

62 Mycobacterium abscessus causes disease in patients with structural abn

63 tidolipid in the outermost portion of the M. abscessus cell wall masks underlying cell wall lipids in

64 s provides an explanation whereby initial M. abscessus colonization of abnormal lung airways escapes

65 hromogenicum (11 of 11), and the M. chelonae-abscessus complex (21 of 21).

66 Multi-drug resistant Mycobacterium abscessus complex (MABSC) is a form of Nontuberculous my

67 equencing (26 isolates of the M. chelonae-M. abscessus complex and 64 remaining isolates, including M

68 Forty-one Mycobacterium abscessus complex isolates from 17 pediatric cystic fibr

69 hods differentiate between members of the M. abscessus complex.

70 farcinica, 12 N. otitidiscaviarum, and 10 N. abscessus cultures were studied.

71 cterium avium complex [MAC] or Mycobacterium abscessus) disease.

72 g susceptibility testing, all isolates of M. abscessus exhibited resistance to tobramycin (MIC of 8 t

73 M. abscessus exists as either a glycopeptidolipid (GPL) exp

74 Conversely, M. abscessus expressing GPL does not stimulate expression o

75 s thought to be colonized with Mycobacterium abscessus for 13 yr prior to developing clinically appar

76 Blood cultures grew M. abscessus for all patients, and admission peripheral blo

77 g were done on 168 consecutive isolates of M abscessus from 31 patients attending an adult cystic fib

78 The release of M. abscessus from apoptotic macrophages initiated the forma

79 -confirmed colonization or infection with M. abscessus from January 2013 through December 2015.

80 cobacteria (RGM), particularly Mycobacterium abscessus, from individuals with cystic fibrosis (CF) is

81 is facilitated by biofilm formation, with M. abscessus glycopeptidolipids playing an important role.

82 Members of the Mycobacterium abscessus group (MAG) cause lung, soft tissue, and disse

83 e M. avium complex (MAC), the M. chelonae-M. abscessus group (MCAG), the M. fortuitum group (MFG), an

84 bscessus (M. abscessus sensu lato, or the M. abscessus group) comprises three closely related taxa wh

85 Mycobacterium massiliense (Mycobacterium abscessus group) is an emerging pathogen causing pulmona

86 e erm(41) and rrl genes in the Mycobacterium abscessus group, a multiplex real-time PCR assay for cla

87 cation within 24 h after isolation of the M. abscessus group.

88 ugh colony morphology shift in Mycobacterium abscessus has been implicated in loss of glycopeptidolip

89 Mycobacterium abscessus has emerged as a major pathogen in cystic fibr

90 Mycobacterium abscessus has emerged as an important cause of lung infe

91 ncluding M. immunogenum, M. chelonae, and M. abscessus, have been associated with nosocomial infectio

92 to define the mechanisms of acquisition of M abscessus in individuals with cystic fibrosis.

93 The incidence rate of M. abscessus increased from 0.7 cases per 10000 patient-day

94 cording in the in vivo physiopathology of M. abscessus infection and emphasizes cording as a mechanis

95 from nine non-CF patients with persistent M. abscessus infection were characterized by colony morphol

96 3 drugs usually combined for treatment of M. abscessus infection, cefoxitin was the most active becau

97 Alternative routes of acquisition of M. abscessus infection, in particular the environment, requ

98 al isolates, we show that the majority of M. abscessus infections are acquired through transmission,

99 The prognosis of Mycobacterium abscessus infections is poor due to the lack of effectiv

100 d chemical structure class active against M. abscessus infections with promising translational develo

101 The same has been observed for M. abscessus infections, which are very difficult to treat;

102 macrolides for treating M. abscessus subsp. abscessus infections.

103 Mycobacterium abscessus is a fast-growing, multidrug-resistant organis

104 Mycobacterium abscessus is a rapidly growing environmental mycobacteri

105 Mycobacterium abscessus is a rapidly growing mycobacterial species whi

106 Mycobacterium abscessus is a rapidly growing Mycobacterium causing a w

107 Mycobacterium abscessus is an important cause of water-related nosocom

108 differentiation of these two species from M. abscessus is difficult and relies on the sequencing of o

109 Mycobacterium abscessus is resistant to multiple antibiotics, creating

110 Mycobacterium abscessus is the most common cause of rapidly growing my

111 It appears that M. abscessus is transported to the CNS within macrophages.

112 activity in vitro against a wide panel of M. abscessus isolates and in infected macrophages.

113 ed whole-genome sequencing of 11 clinical M. abscessus isolates derived from eight U.S. patients with

114 -genome sequencing data demonstrated that M. abscessus isolates from 16 patients were unrelated, diff

115 The M. abscessus isolates from case patients and the supplement

116 Whole-genome sequencing was applied to 27 M. abscessus isolates from the 20 patients in this cohort t

117 liense and 15% to 20% of M. abscessus subsp. abscessus isolates renders these species intrinsically m

118 .S. and Western European M. abscessus subsp. abscessus isolates that are genetically distinct from ot

119 Previous population studies of clinical M. abscessus isolates utilized multilocus sequence typing o

120 d to the unambiguous identification of 26 M. abscessus isolates, 7 M. massiliense isolates, and 2 M.

121 testing by Etest of four carbapenems for M. abscessus isolates.

122 coside resistance levels of 50 Mycobacterium abscessus isolates.

123 rdonae, and 5 M. chelonae group (all were M. abscessus) isolates.

124 nd mitigated a 2-phase clonal outbreak of M. abscessus linked to hospital tap water.

125 cal to the CLSI guidelines for Mycobacterium abscessus: </=16 mug/ml for susceptible, 32 mug/ml for i

126 Mycobacterium abscessus (M. abscessus sensu lato, or the M. abscessus

127 ough, wild-type human clinical isolate of M. abscessus (M. abscessus-R) and a smooth, attenuated muta

128 PCR for straightforward identification of M. abscessus, M. massiliense, and M. bolletii and the asses

129 ion and typing of 42 clinical isolates of M. abscessus, M. massiliense, and M. bolletii from patients

130 PCR-based method for distinguishing among M. abscessus, M. massiliense, and M. bolletii.

131 ates with ambiguous species identities as M. abscessus-M. massiliense by rpoB, hsp65, and secA sequen

132 verscores resistance and that isolates of M. abscessus/M. chelonae from CF patients are more likely t

133 Mycobacterium abscessus (Mabs) is a rapidly growing Mycobacterium and

134 also in few samples containing Mycobacterium abscessus,Mycobacterium gordonae, o rMycobacterium therm

135 acteria (41%), fungi (10%) and Mycobacterium abscessus, Mycoplasma hominis and Lactobacillus sp. (one

136 ubsp. bolletii (n = 24), M. abscessus subsp. abscessus (n = 6), Mycobacterium fortuitum (n = 3), Myco

137 re used to identify 75 isolates as either M. abscessus or M. chelonae that were originally submitted

138 who presented with multifocal Mycobacterium abscessus osteomyelitis (patient 1) and disseminated CMV

139 ulous mycobacteria, especially Mycobacterium abscessus, post-transplantation survival has not been de

140 We studied 118 strains of M. abscessus previously studied by PFGE by randomly amplifi

141 erved that the increased virulence of the M. abscessus R variant compared with the S variant correlat

142 ast-mycobacterium microcolony assay, with M. abscessus-R exhibiting growth characteristics similar to

143 phagocyte aggregates develop at sites of M. abscessus-R infection, but are absent with M. abscessus-

144 We have found that M. abscessus-R is able to persist and multiply in a murine

145 M. abscessus-R is able to persist and multiply in human mon

146 M. abscessus-R resides in a phagosome typical for pathogeni

147 e human clinical isolate of M. abscessus (M. abscessus-R) and a smooth, attenuated mutant (M. abscess

148 Recently, two new M. abscessus-related species, M. massiliense and M. bolleti

149 The M. abscessus rough (R) variant, devoid of cell-surface glyc

150 reported for virulent M. tuberculosis and M. abscessus-S exhibiting growth characteristics similar to

151 bscessus-R infection, but are absent with M. abscessus-S infection.

152 st and multiply in human monocytes, while M. abscessus-S is deficient in this ability.

153 In contrast, M. abscessus-S resides in a "loose" phagosome with the phag

154 clude that a mutation has occurred in the M. abscessus-S variant which has altered the ability of thi

155 essus-R) and a smooth, attenuated mutant (M. abscessus-S) which spontaneously dissociated from the cl

156 pulmonary infection model in contrast to M. abscessus-S, which is rapidly cleared.

157 Mycobacterium abscessus (M. abscessus sensu lato, or the M. abscessus group) compris

158 onomic statuses are under revision, i.e., M. abscessus sensu stricto, Mycobacterium bolletii, and Myc

159 cobacterium immunogenum, M. chelonae, and M. abscessus, showed various susceptibilities to the glutar

160 udy we demonstrate that rough variants of M. abscessus stimulate the human macrophage innate immune r

161 eport herein the draft genome sequence of M. abscessus strain 47J26.

162 ern) occurs with almost 50% of Mycobacterium abscessus strains during pulsed-field gel electrophoresi

163 xplores the genomic diversity of clinical M. abscessus strains from multiple continents and provides

164 om 3 confirmed infections grew Mycobacterium abscessus strains that were indistinguishable by pulsed-

165 PCR can be used for genetic comparison of M. abscessus strains, including strains which cannot be com

166 of a patient with disseminated Mycobacterium abscessus, Streptococcus viridians bacteremia, and cytom

167 erium abscessus subsp. bolletii (n = 24), M. abscessus subsp. abscessus (n = 6), Mycobacterium fortui

168 tedizolid MIC90 values for 81 isolates of M. abscessus subsp. abscessus and 12 isolates of M. abscess

169 bases accurately identified 41 Mycobacterium abscessus subsp. abscessus and 13 M. abscessus subsp. ma

170 ted the presence of two clonal groups for M. abscessus subsp. abscessus and five clonal groups for M.

171 ithromycin susceptibility breakpoints for M. abscessus subsp. abscessus be changed from </=2 to </=4

172 the usefulness of macrolides for treating M. abscessus subsp. abscessus infections.

173 ssus subsp. massiliense and 15% to 20% of M. abscessus subsp. abscessus isolates renders these specie

174 closely related U.S. and Western European M. abscessus subsp. abscessus isolates that are genetically

175 Sequencing of the erm gene of M. abscessus subsp. abscessus will predict inducible macrol

176 ucible macrolide resistance in Mycobacterium abscessus subsp. abscessus, calling into question the us

177 ing approximately 20% of U.S. isolates of M. abscessus subsp. abscessus.

178 The species identified were Mycobacterium abscessus subsp. bolletii (n = 24), M. abscessus subsp.

179 eregrinum and a nonfunctional erm gene in M. abscessus subsp. massiliense and 15% to 20% of M. absces

180 cterium abscessus subsp. abscessus and 13 M. abscessus subsp. massiliense isolates identified by whol

181 essus subsp. abscessus and 12 isolates of M. abscessus subsp. massiliense were 8 mug/ml and 4 mug/ml,

182 All 50 isolates were typed as M. abscessus subspecies abscessus and were clonally related

183 utbreaks of near-identical isolates of the M abscessus subspecies massiliense (from 11 patients), dif

184 The clusters of M abscessus subspecies massiliense showed evidence of tran

185 ients with genetically related strains of M. abscessus that had been previously typed by variable-num

186 The intrinsic resistance of M. abscessus to most commonly available antibiotics serious

187 smear patterns were identical to those of M. abscessus type strain ATCC 19977, which had a nonsmear p

188 Both M. abscessus variants also have distinctive growth patterns

189 t respiratory epithelial cells respond to M. abscessus variants lacking GPL with expression of IL-8 a

190 Mycobacterium abscessus was also isolated from respiratory specimens (

191 . abscessus, and one isolate submitted as M. abscessus was found to be M. chelonae.

192 Previously, M. abscessus was thought to be independently acquired by su

193 y in M. chelonae), and cefoxitin (only in M. abscessus) was shown.

194 M. avium, M. mucogenicum, and Mycobacterium abscessus were found to persist most frequently.

195 ncident NTM infections from either MAC or M. abscessus were less likely to have had chronic azithromy

196 eorgica, N. asteroides, N. farcinica, and N. abscessus were only moderate resistant.

197 Several bacterial species, including M. abscessus, were cultured from an opened multidose supple

198 Five outbreaks due to M. abscessus which gave broken DNA by PFGE gave evaluable p

199 uberculous mycobacterium (NTM) Mycobacterium abscessus, which causes progressive lung damage and is e

200 ncing of the erm gene of M. abscessus subsp. abscessus will predict inducible macrolide susceptibilit

201 ve not demonstrated cross-transmission of M. abscessus within our hospital, except between 1 sibling