ライフサイエンスコーパス: acetate kinase

1 ate, supports a direct in-line mechanism for acetate kinase.

2 roach has only recently become available for acetate kinase.

3 te and ATP synthesis through the activity of acetate kinase.

4 hat EutQ and EutP represent novel classes of acetate kinases.

5 Acetate kinase, a member of the acetate and sugar kinase

6 Archaeal genus that can utilize acetate via acetate kinase (Ack) and phosphotransacetylase (Pta).

7 r genes (those encoding the Escherichia coli acetate kinase (ACK) and Tn9 chloramphenicol, acetyl tra

8 Acetate kinase (ACK; E.C. 2.7.2.1), which catalyzes the

9 tate via the phosphotransacetylase (pta) and acetate kinase (ackA) genes while downregulating express

10 aboratory has shown that the gene coding for acetate kinase (ackA) in Sinorhizobium meliloti is up-re

11 ermediate of the phosphotransacetylase (Pta)-acetate kinase (AckA) pathway, can activate the transcri

12 Herein we show that both proteins have acetate kinase activity and that EutQ is required during

13 Although EutP had acetate kinase activity, DeltaeutP strains lacked discer

14 MgCl(2), AlCl(3), NaF, and acetate, inhibit acetate kinase activity.

15 The bacterial enzymes acetate kinase (AK) and phosphotransacetylase (PTA) form

16 genic step of AD with a focus on the role of acetate kinase (AK), which is a key enzyme in methane pr

17 Acetate kinase, an enzyme widely distributed in the Bact

18 and ack) encoding phosphotransacetylase and acetate kinase and is transcribed in the opposite direct

19 tion of acetate to induce gene expression by acetate kinase and part of the nitrogen-regulation two-c

20 ld-type strain eliminated elevated levels of acetate kinase and phosphotransacetylase activities in r

21 We found that a mutant with deletions of the acetate kinase and phosphotransacetylase genes (ackA-pta

22 Pyruvate formate lyase, acetate kinase, and hydrogenase protein levels were simi

23 Thus, pyruvate formate lyase and acetate kinase are essential for anaerobic growth of E.

24 onal structure of Methanosarcina thermophila acetate kinase bound to ADP through crystallography.

25 ed active-site residues are conserved within acetate kinases, but few are conserved within the phosph

26 he results are discussed with respect to the acetate kinase catalytic mechanism and the relationship

27 Acetate kinase catalyzes phosphoryl transfer of the ATP

28 Acetate kinase catalyzes the magnesium-dependent transfe

29 Acetate kinase catalyzes the reversible magnesium-depend

30 Acetate kinase catalyzes the reversible phosphorylation

31 Acetate kinase catalyzes transfer of the gamma-phosphate

32 As we previously predicted, acetate kinase contains a core fold that is topologicall

33 sertion mutants disrupted for genes encoding acetate kinases (EC 2.7.2.1) (ACK1 and ACK2) and a phosp

34 pathway involving phosphotransacetylase and acetate kinase, encoded by pta and ackA, respectively.

35 he operon encoding phosphotransacetylase and acetate kinase failed to use either acetate or CO as gro

36 confirming the need for the ATP produced by acetate kinase for anaerobic growth on xylose.

37 indicated an increase in binding affinity of acetate kinase for MgADP in the presence of AlCl(3), NaF

38 Here we describe the inhibition of acetate kinase from Methanosarcina thermophila by preinc

39 e of histidine in the catalytic mechanism of acetate kinase from Methanosarcina thermophila was inves

40 lignment of the amino acid sequences for the acetate kinases from E. coli (Bacteria domain), Methanos

41 res acetyl phosphate levels by the action of acetate kinase, further suggesting that spxB encodes a p

42 Transcription of the acetate kinase gene (ackA) proved to be activated as wel

43 ologous expression and activity assay of the acetate kinase gene ack from Bathyarchaeota, demonstrate

44 n their high conservation among sequences of acetate kinase homologues.

45 an origin hypothesis does not exist for the acetate kinase in Bacteria, Archaea, or Eukarya.

46 he decreased ATP availability resulting from acetate kinase inactivation.

47 The only crystal structure reported for acetate kinase is the homodimeric enzyme from Methanosar

48 the phosphoryl transfer mechanisms, that of acetate kinase, is not completely understood.

49 des better understanding of the mechanism of acetate kinase, knowledge of the structure of butyrate k

50 geochemical considerations indicate that an acetate kinase may be the ancestral enzyme of the ASKHA

51 ate to acetate via the phosphotransacetylase-acetate kinase pathway generates ATP and is a major over

52 xcrete acetate via the phosphotransacetylase-acetate kinase pathway.

53 of genes involved in organic acid formation (acetate kinase, phosphate acetyltransferase, and L-lacta

54 Alignments of acetate kinase, propionate kinase, and butyrate kinase s

55 m, suggesting that the phosphotransacetylase-acetate kinase (Pta-AckA) pathway plays a crucial role i

56 xcrete acetate via the phosphotransacetylase-acetate kinase (Pta-AckA) pathway.

57 Comparison of the M. thermophila acetate kinase sequence with 56 putative acetate kinase

58 te was further supported by alignment of all acetate kinase sequences available from databases, which

59 ila acetate kinase sequence with 56 putative acetate kinase sequences revealed eight highly conserved

60 edes a conserved glycine residue (Gly-331 in acetate kinase) that binds the alpha-phosphate of ADP.

61 The role of arginines in this acetate kinase was investigated.

62 The unaltered acetate kinase was not inactivated by N-ethylmaleimide;

63 of the Bacillus subtilis ackA gene, encoding acetate kinase, was previously shown to require cataboli

64 from four of these motifs in M. thermophila acetate kinase were selected for site-directed replaceme

65 Synthesis of acetate kinase, which is involved in the conversion of a