ライフサイエンスコーパス: acetoacetyl

1 er protein (ACP) to make the FAS primer beta-acetoacetyl-ACP in the accompanying article.

2 The radiolabeled acetoacetyl-ACP product is precipitated and separated fr

3 ein (ACP) synthase III (KAS III, also called acetoacetyl-ACP synthase) encoded by the fabH gene is th

4 ACP) allows the generation of a biotinylated acetoacetyl-ACP.

5 ularly against the KASIII domain of the beta-acetoacetyl-acyl carrier protein (ACP) synthase FabH.

6 alonyl-CoA derived extender unit to yield an acetoacetyl-, beta-ketopentanoyl-, 3-oxo-4-methylpentano

7 role of AACT2 in generating the bulk of the acetoacetyl CoA precursor required for the cytosol-local

8 Acetoacetyl CoA thiolase (AACT, EC 2.3.1.9) catalyzes th

9 , acetyl CoA, crotonoyl CoA, n-propzoyl CoA, acetoacetyl CoA, malonyl CoA) were completely separated

10 ensation of two acetyl CoA molecules to form acetoacetyl CoA.

11 by monitoring the use of a second substrate, acetoacetyl-CoA (300 nm).

12 n solution, where HMG-CoA is cleaved to form acetoacetyl-CoA (AcAc-CoA) and acetate.

13 netic and chemical mechanisms of KACPR using acetoacetyl-CoA (AcAc-CoA) as a substrate.

14 imilar K(m) values for binding of substrates acetoacetyl-CoA (K(m) 9.8 +/- 0.8 microM) and CoA (K(m)

15 DH II inhibited, in parallel, reduction of S-acetoacetyl-CoA (Ki approximately 1.6 microM), as well a

16 tructure of E170Q in complex with NAD(+) and acetoacetyl-CoA (R = 21.9%, R(free) = 27.6%, 2.2 A) reve

17 ossessed virtually unchanged K(m) values for acetoacetyl-CoA and CoA but had a greater than 99% decre

18 t in opposite directions) for the binding of acetoacetyl-CoA and indoleacryloyl-CoA to the enzyme.

19 h apparent Km values of 89 and 20 microM for acetoacetyl-CoA and NADH, respectively.

20 eneral base both in the condensation between acetoacetyl-CoA and the acetylated enzyme, and the hydro

21 The acetoacetyl-CoA binary structure demonstrates reduced co

22 contrast, abstraction of the alpha-proton of acetoacetyl-CoA by Arg-94 --> Gln mutant dehydrogenase i

23 ed HMGS from Staphylococcus aureus and bound acetoacetyl-CoA by cryo-cooling enzyme crystals at three

24 off-rate" of acetoacetyl-CoA from the enzyme-acetoacetyl-CoA complex.

25 argely attributed to a decreased affinity of acetoacetyl-CoA for these enzymes and, more specifically

26 n increase in the dissociation "off-rate" of acetoacetyl-CoA from the enzyme-acetoacetyl-CoA complex.

27 A dehydrogenase complexed with the inhibitor acetoacetyl-CoA has been determined at 2.25 A resolution

28 Succinyl-CoA and acetoacetyl-CoA increased the rate of glycine proton rem

29 catalyzes the condensation of acetyl-CoA and acetoacetyl-CoA into 3-hydroxy-3-methylglutaryl CoA.

30 athway is the condensation of acetyl-CoA and acetoacetyl-CoA into HMG-CoA, catalyzed by the enzyme HM

31 initially reacts with acetoacetate to yield acetoacetyl-CoA plus succinate in the succinyl-CoA-aceto

32 This would be followed by acetoacetyl-CoA reacting with acetyl-CoA to generate HMG

33 catalyzed by a beta-ketothiolase (PhaA), an acetoacetyl-CoA reductase (PhaB), and a polyhydroxyalkan

34 etic fragment encoding beta-ketothiolase and acetoacetyl-CoA reductase behind a modified synthase gen

35 haB2 and phaB3 as well as 15 other potential acetoacetyl-CoA reductases.

36 se-catalyzed condensation of acetyl-CoA with acetoacetyl-CoA requires enolization/carbanion formation

37 ydrogenase in cultured skin fibroblasts with acetoacetyl-CoA substrate showed reduced activity.

38 zation, and a new acetylation target, namely acetoacetyl-CoA synthetase (SlAacS).

39 However, lowering acetoacetyl-CoA synthetase 80% partially inhibited gluco

40 sent a conserved mechanism for regulation of acetoacetyl-CoA synthetase activity in all domains of li

41 succinyl-CoA:3-ketoacid-CoA transferase and acetoacetyl-CoA synthetase to synthesize and use acetoac

42 forms acetoacetate in the mitochondria, and acetoacetyl-CoA synthetase, which uses acetoacetate to f

43 ivo evidence show that SlAacS is a bona fide acetoacetyl-CoA synthetase.

44 d more forcefully to regulatory stimuli than acetoacetyl-CoA thiolase activity but usually less than

45 6.5-kDa mvaE gene product catalyzed both the acetoacetyl-CoA thiolase and HMG-CoA reductase reactions

46 Under conditions at which acetoacetyl-CoA thiolase and long-chain thiolase were co

47 g as a selective SNO-CoA reductase, protects acetoacetyl-CoA thiolase from inhibitory S-nitrosylation

48 Kinetic studies of acetoacetyl-CoA thiolase implicated a ping-pong mechanis

49 s demonstrated by using HMG-CoA synthase and acetoacetyl-CoA thiolase/HMG-CoA reductase from E. faeca

50 d mitochondrial thiolases, as well as to the acetoacetyl-CoA thiolases of prokaryotes.

51 the kinetics of the reaction that have shown acetoacetyl-CoA to be a potent inhibitor of the overall

52 r) is crotonyl-CoA reductase, which converts acetoacetyl-CoA to butyryl-CoA for use as a 4C extender

53 acetyl-CoA, which precedes condensation with acetoacetyl-CoA to form the HMG-CoA product.

54 the DeltaH degrees value for the binding of acetoacetyl-CoA to the enzyme was 5.6 kcal/mol more favo

55 a crystal structure of HMG-CoA synthase with acetoacetyl-CoA was determined at 2.5-A resolution.

56 differences in (13)C NMR chemical shifts for acetoacetyl-CoA when bound as an enolate to MCAD and eno

57 catalyzed the NADH-dependent reduction of S-acetoacetyl-CoA with a Km of approximately 68 microM and

58 r H264 interacts with the carbonyl oxygen of acetoacetyl-CoA's thioester, turnover of S-(3-oxobutyl)-

59 of acetyl phosphate, acetoacetyl coenzyme A (acetoacetyl-CoA), butyryl CoA, acetoacetate, and beta-hy

60 st that, in the absence of second substrate (acetoacetyl-CoA), enzymatic addition of H(2)(18)O to the

61 nt types of CoA-ligands (viz., octenoyl-CoA, acetoacetyl-CoA, and indoleacryloyl-CoA) to human liver

62 rporate carbon from glucose into acetyl-CoA, acetoacetyl-CoA, and succinyl-CoA and carbon from leucin

63 le substrate analogs, 3-thiaglutaryl-CoA and acetoacetyl-CoA, are not altered by the mutations.

64 ay a role in anchoring the second substrate, acetoacetyl-CoA, by interacting with the carbonyl oxygen

65 in formation of a stable binary complex with acetoacetyl-CoA, F204L exhibits binding stoichiometries

66 of the purine and nicotinamide nucleotides, acetoacetyl-CoA, H2O2, reduced glutathione, and 2-monoac

67 ndensation of two molecules of acetyl-CoA to acetoacetyl-CoA, is thermodynamically unfavorable.

68 ondria and enzymes that can form acetyl-CoA, acetoacetyl-CoA, malonyl-CoA, and HMG-CoA in their cytos

69 ass spectrometry measurements of acetyl-CoA, acetoacetyl-CoA, succinyl-CoA, hydroxymethylglutaryl-CoA

70 s acutely stimulated 1.5-5-fold increases in acetoacetyl-CoA, succinyl-CoA, malonyl-CoA, hydroxymethy

71 In the case of acetoacetyl-CoA, the spectrum of the enzyme-ligand compl

72 enzyme complexed with its second substrate, acetoacetyl-CoA, to 1.9 A.

73 of S-(3-oxobutyl)-CoA, a thioether analog of acetoacetyl-CoA, was investigated.

74 acetyl-S-enzyme during its condensation with acetoacetyl-CoA.

75 y E95Q was not stimulated in the presence of acetoacetyl-CoA.

76 ernary complex of the enzyme with NAD(+) and acetoacetyl-CoA.

77 ensitive to feedback substrate inhibition by acetoacetyl-CoA.

78 of S-3-oxobutyl-CoA, the thioether analog of acetoacetyl-CoA.

79 roximately 100-fold increase in the k(m) for acetoacetyl-CoA.

80 nsible for the formation of butyryl-CoA from acetoacetyl-CoA.

81 arent k(m) values for S-(3-oxobutyl)-CoA and acetoacetyl-CoA.

82 obtained using the physiological substrate, acetoacetyl-CoA.

83 Escherichia coli cells that overexpress the acetoacetyl-CoA:acetyl-CoA transferase, AtoAD (EC 2.8.3.

84 h microsomal HMG-CoA reductase and cytosolic acetoacetyl coenzyme A (AcAc-CoA) thiolase activities.

85 e, and increased levels of acetyl phosphate, acetoacetyl coenzyme A (acetoacetyl-CoA), butyryl CoA, a

86 oding beta-ketothiolase (phaA), NADPH-linked acetoacetyl coenzyme A (acetyl-CoA) reductase (phaB), an

87 strate that FadA5 catalyzes the thiolysis of acetoacetyl-coenzyme A (CoA).

88 obacterium tuberculosis and its complex with acetoacetyl-coenzyme A at 1.8 and 2.3 A resolution, resp

89 express in transgenic cotton genes encoding acetoacetyl-coenzyme A reductase and polyhydroxyalkanoic

90 ncode two enzymes of the mevalonate pathway, acetoacetyl-coenzyme A thiolase and 3-hydroxy-3-methylgl

91 haracterizations of iso-CoA, acetyl-iso-CoA, acetoacetyl-iso-CoA, and beta-hydroxybutyryl-iso-CoA usi

92 ase (PKS-NRPS) that makes and releases cyclo-acetoacetyl-L-tryptophan (cAATrp), the tetramic acid tha

93 e synthetase (PKS-NRPS) that generates cyclo-acetoacetyl-L-tryptophan (cAATrp).

94 an acetate-derived beta-methyl branch on an acetoacetyl-S-carrier protein and ultimately generate a

95 alyzes a Dieckmann-type cyclization on the N-acetoacetyl-Trp intermediate bound in thioester linkage