ライフサイエンスコーパス: acidic residue

1 motifs cannot be fully substituted by other acidic residue.

2 igh-affinity FXR modulator not comprising an acidic residue.

3 no group of the bound amino acid by using an acidic residue.

4 linical strains, and to mutagenize conserved acidic residues.

5 for short peptide substrates with N-terminal acidic residues.

6 tor is coordinated by three highly conserved acidic residues.

7 t not N-terminal) EH-domains are followed by acidic residues.

8 nced ability to glycosylate sites flanked by acidic residues.

9 light conditions and sensed by lumen-exposed acidic residues.

10 His90 orient their side chains toward these acidic residues.

11 n Ca(2+) is absent) as H(+) dissociates from acidic residues.

12 than a formal negative charge from proximal acidic residues.

13 ding requires neutralization of a cluster of acidic residues.

14 ssential for aldolase binding, and clustered acidic residues.

15 ide-chains from the zipper and two invariant acidic residues.

16 lation sites can functionally substitute for acidic residues.

17 eta30 polymorphisms and had low tolerance of acidic residues.

18 rrent MCO/MS protocol is transparent to such acidic residues.

19 catalytic domain and is highly enriched with acidic residues.

20 of the cytoplasmic domain, which is rich in acidic residues.

21 be linked to the staggered titration of key acidic residues.

22 nine 51 (Met51) with upstream and downstream acidic residues.

23 omerases, with an active site clustered with acidic residues.

24 basic residue interacts with only one of the acidic residues.

25 d in a molecular surface that is coated with acidic residues, a marked difference from beta-lactamase

26 Q AceI mutant with a mutation in a conserved acidic residue, although unable to mediate chlorhexidine

27 The enzyme contains a number of conserved acidic residues and a cysteine- and arginine-rich motif,

28 multidomain extracellular region enriched in acidic residues and carboxyl-carboxylate pairs within 3

29 sequence features indicated a preference for acidic residues and increased hydrophilicity in the regi

30 h water and participate in salt bridges with acidic residues and lipid phosphate groups.

31 Each monomer includes 34 acidic residues and only six basic residues.

32 in an inner chamber that contains a ring of acidic residues and that is near the channel entrance; t

33 rom site-specific mutations of the conserved acidic residues and the arginine residues in the iron-su

34 Between the acidic residues and the transmembrane pore lies a disulp

35 These two acidic residues and this H-bond network appear to distin

36 YYEDSYED) within this region consist of five acidic residues and three sulfo-Tyr residues, thus repre

37 In an iterative process, we identified two acidic residues and two hydrophobic residues that form t

38 nts a hydrophobic residue, Phi is usually an acidic residue, and X is any residue) and able to inhibi

39 is change was due to a pK(a) shift of site I acidic residues, and to contributions of oxygen function

40 Mechanistically, the acidic residues are important in enhancing the efficienc

41 e form of gelsolin shows that the equivalent acidic residues are in close contact with G3, which may

42 mplex, while Arg(6), His(11), and C-terminal acidic residues are involved in stabilizing the final co

43 , enolase, and C-Raf) show that the relevant acidic residues are present in salt bridges with conserv

44 We found that acidic residues are required in LF(N) to utilize a proto

45 It is commonly believed that acidic residues are required near the orifice of such pr

46 The presence of acidic residues Asp and Glu near the peptide N-terminus

47 The carboxylate moieties of acidic residues Asp-109, Asp-207, Asp-298, and Asp-337 i

48 onal NOS (nNOSr) contains a widely conserved acidic residue (Asp(1393)) that is thought to facilitate

49 erminal site (amino acids 108-130) and by an acidic residue (Asp(641)) at the pore loop of TRPV3.

50 xternal site is under indirect control by an acidic residue (Asp-66), making it Ca(2+)-specific.

51 and S/T+1 positions, but strongly disfavors acidic residues (Asp or Glu) at the S/T-2 position and a

52 stabilizes the structure by coordinating two acidic residues (Asp(552) and Glu(648)), one from each o

53 We found that acidic residues (Asp-83 and Asp-84) in G1 of GSNL-1 are

54 ng group-accommodating groove, and a pivotal acidic residue, Asp-123, after the nucleophile Ser-122.

55 -chain carboxylate groups from the conserved acidic residue, Asp-66 in NaK, conventionally thought to

56 Furthermore, mutation of two nearby acidic residues, Asp(367) and Glu(399), also reduced act

57 quence alignments identified three conserved acidic residues, Asp-166, Glu-167, and Glu-222, that are

58 trescine binds in a buried pocket containing acidic residues Asp174, Glu178, and Glu256.

59 A subset of mutations of a conserved acidic residue (Asp43 in Escherichia coli alpha) present

60 uires ribosome binding and is mediated by an acidic residue, Asp924, near the center of the putative

61 Two buried acidic residues assist in the reaction.

62 bc15 largely results from the presence of an acidic residue at its N-terminal region.

63 rophobic residue at the P-2 position plus an acidic residue at the P-3 position (class II) can also o

64 a conserved inner cavity position, although acidic residues at any pore-lining position in the inner

65 irect substitution or suppressor generation, acidic residues at one or both positions of the H168/H31

66 Finally, we show that Smo containing acidic residues at PKA and CK1 sites can be stimulated f

67 served arginine at position +1 and conserved acidic residues at positions -2 and/or -3 are required f

68 uggests that the hydrolysis is determined by acidic residues at the active site.

69 domain and bind to C-terminal sequences with acidic residues at the P-2 position with low micromolar

70 In addition, the human MetAPs disfavor acidic residues at the P2'-P5' positions.

71 evealing a previously unknown preference for acidic residues at the substrate prime site position P1'

72 ot exhibit preferential cleavage adjacent to acidic residues but did show enhancement next to proline

73 AAV2 and AAV4 showed a higher propensity of acidic residues but similar volumes, consistent with com

74 ein, we determined the role of the conserved acidic residues by using alanine point mutants to invest

75 Our studies revealed two separate acidic residue clusters in the cytoplasmic domain of MIC

76 the presequence is optimized in its natural acidic residue content for efficient DeltapH-driven unfo

77 h basic and acidic residues or predominantly acidic residues contribute to actin affinity.

78 with TRPV1 mutants identified extracellular acidic residues critical for polyamine regulation.

79 Acidic residues critical to protein function were identi

80 which are characterized by multiple flanking acidic residues, CTRC shows substrate specificity that i

81 To that end, acidic residues, D and E, and their neutral amides, N an

82 In addition to the aromatic amino acids, two acidic residues, D111 and E113, form salt bridges with b

83 mutation that mimics protonation of the key acidic residue (D158N) was shown to trigger a global con

84 ve centre has a Mg ion co-ordinated by three acidic residues, D401, E458 and D542.

85 high-affinity interaction with the innermost acidic residue (D523) of the selectivity filter and subs

86 lytic core comprised of the highly conserved acidic residues D524, E562, and D583.

87 d that 2 of the 8 MiRP2 extracellular domain acidic residues (D54 and D55) are important for KCNQ1-Mi

88 egion to be critical in DNA binding, whereas acidic residues D88 and E89 (wing), D8 and E11 (metal-bi

89 ong with an arginine and a second cluster of acidic residues defining the other prong.

90 ree proteins where phosphosites evolved from acidic residues (DNA topoisomerase II, enolase, and C-Ra

91 otif (NDSM) carries an additional stretch of acidic residues downstream of the consensus Psi-K-x-E/D

92 moval of the CTD or mutations that eliminate acidic residues dramatically increase the protein/DNA in

93 opose a model in which Arg(6) and N-terminal acidic residues drive the encounter complex, while Arg(6

94 lectrophysiological data have shown that the acidic residues E106 in transmembrane helix 1 (TM1) and

95 G465D and K486N, and by single mutations in acidic residues E460 and D461.

96 (D641) in the extracellular pore loop or two acidic residues (E679, E682) in the inner pore region si

97 , whereas the combined neutralization of six acidic residues eliminated inactivation entirely.

98 demonstrates that the Glu(309) and Glu(771) acidic residues (empty Ca(2+)-binding sites I and II) ar

99 ages N-terminal to proline and C-terminal to acidic residues (especially for the lowest charge states

100 II substrates typically contain two or more acidic residues, especially at Tyr(P)-1 to Tyr(P)-3 posi

101 pendent on glutamate, thus establishing that acidic residues facilitate lysine modification and form

102 is, and NMR to demonstrate that the flanking acidic residues "fine tune" the binding affinity to EHD1

103 The acidic residues flanking this basic cluster in PLC-zeta

104 A long loop (L3) carrying multiple acidic residues folds into the beta-barrel pore to form

105 rence for either a hydrophobic residue or an acidic residue following the aromatic residue in the LIR

106 The results indicate that the targeting of acidic residues for cross-linking provides distance rest

107 ronegative potential of B-NRP2 by exchanging acidic residues for uncharged alanine (B-NRP2 E284A,E291

108 Three conserved acidic residues form a pyramidal-shaped cation-binding s

109 ultiple ion-binding sites, and the conserved acidic residues form the luminal Ca(2+)-blocking site th

110 in and is adjacent to a cluster of conserved acidic residues found in diflavin oxidoreductases.

111 ily enzymes, and the catalytically important acidic residues found in family 43 enzymes were conserve

112 lution in which codons for the corresponding acidic residues found in Ocr were specifically re-introd

113 tical: three conserved, calcium-coordinating acidic residues from each LDLR module encircle a lysine

114 oprotease that cleaves aspartic and glutamic acidic residues from the N-terminus of a number of prote

115 ine in JKJ05, which appears to interact with acidic residue Glu(767) in our model of the seven-transm

116 The acidic residue Glu-395 located at the active site of PPO

117 These elements consist of the acidic residues Glu(341), Asp(348), and Asp(355) located

118 Further, key acidic residues Glu(6), Asp(8), and Glu(16), believed cr

119 n of the Tyr(11) residue of NT[8-13] with an acidic residue (Glu(179)) located in the ECL2 of hNTS2 o

120 Substitution of the three acidic residues (Glu(571), Glu(235), and Glu(237)) and A

121 Furthermore, altering a single acidic residue, Glu-478, on the C-terminal domain to glu

122 Two acidic residues, Glu-48 and Glu-49, of cytochrome b5 (b5

123 Two acidic residues, Glu153 and Asp156, that lie in a conser

124 LeuT are chloride-independent but contain an acidic residue (Glu290 in LeuT) at a site where eukaryot

125 which bridges His143 of cytochrome f and the acidic residue, Glu75, of cyt b(6); in addition, Cd1 is

126 Mutations of arginine 130, 135, and 136 and acidic residues Glu86, Asp165, Glu236, and Asp172 dramat

127 owever, mutations at two conserved nonligand acidic residues, Glu95 and Glu100, result in low metal c

128 function was confirmed by substitution of an acidic residue (glutamate, BRCA2(Deltaex11/S3291E)) for

129 nhibition can be ascribed to the lack of two acidic residues--highly conserved in other ACA isoforms-

130 to an apolar residue or converting V96 to an acidic residue impedes the allosteric response to induce

131 Using Xenopus egg extracts, we identify an acidic residue in PCNA that is essential to support dest

132 stance are mutations of a specific serine or acidic residue in the A subunit of gyrase or topoisomera

133 nsin II receptors and export ability of each acidic residue in the di-acidic motifs cannot be fully s

134 ructural analysis reveals that the conserved acidic residue in the filter is essential for Ca(2+) bin

135 the present study, we identified a conserved acidic residue in the motor domain (Asp-136) and two con

136 two (or more) protons requires an additional acidic residue in the multidrug recognition pocket of Md

137 The final acidic residue in the N-arm that was tested, E15, is sho

138 Successive replacement of acidic residues in Ac-GPSLNPFDEED-NH(2) with neutral res

139 proteins, pK(a) values were measured for the acidic residues in both proteins using (13)C NMR chemica

140 Additionally, acidic residues in Csm5 are required for efficient matur

141 e neutralization mutations of two N-terminal acidic residues in DPP6-S eliminated the increase in gam

142 Interestingly, the acidic residues in helix alpha9a of CeFIGL-1-AAA are not

143 Neutralizing the acidic residues in p6 improves the annealing and aggrega

144 In particular, DR0801 strongly preferred acidic residues in pocket 9.

145 monstrate the variation in importance of the acidic residues in regions of Ocr corresponding to diffe

146 bridge between a residue in the S4 helix and acidic residues in S2.

147 the formation of salt bridges with specific acidic residues in SH3 domains.

148 Basic residues in site 1 and acidic residues in site 2 were essential for positive re

149 ly 33 %) due to the protonation of a few key acidic residues in the A and B strands.

150 primarily stabilized by interactions between acidic residues in the ACP helix alpha2 and a patch of b

151 Acidic residues in the activation domain of protein C ar

152 We have identified four conserved acidic residues in the active site that are necessary fo

153 rimetry and NMR spectroscopy, we report that acidic residues in the BAZ2B PHD domain are essential fo

154 Acidic residues in the C-terminal linker of the E2b lipo

155 acidic cluster sorting protein-2 (PACS-2) to acidic residues in the carboxyterminal tail of K5.

156 f EmrE subsequent to protonation of critical acidic residues in the context of a global description o

157 Acidic residues in the core of the E1b heterotetramer, w

158 eractions between TRAF2 and GITR mediated by acidic residues in the cytoplasmic domain of the recepto

159 functional effects are caused by mutation of acidic residues in the cytoplasmic segment of Orai1/CRAC

160 estigated the contribution of the cluster of acidic residues in the distal C terminus of TRPA1 in the

161 analysis and revealed a pattern of basic and acidic residues in the first halves of the periodic repe

162 Replacement of the acidic residues in the flexible linker with alanine elev

163 nditions, implying that the highly conserved acidic residues in the gamma subunit influence thiol red

164 Of 11 acidic residues in the I-domain, seven participate in st

165 round the canonical cleft, especially to the acidic residues in the loops, as revealed by NMR titrati

166 esidues 316-326) interacts with a cluster of acidic residues in the middle of helix 4.

167 the interaction of scaffolding protein with acidic residues in the N-arm of coat protein, since this

168 tutions in betaS, we localized the switch to acidic residues in the N-terminal and non-amyloid compon

169 We show that mutation of acidic residues in the N-terminal helix impair the abili

170 thermore, protonation-mimicking mutations of acidic residues in the palm induce a dramatic accelerati

171 ntaining the peptide length but substituting acidic residues in the peptide with neutral or basic res

172 gated the role of electrostatic repulsion of acidic residues in the pH-dependent large conformational

173 asured at pH 5.2, suggesting the presence of acidic residues in the pore.

174 gating charge in the S4 segment (R4) and two acidic residues in the S2 segment during activation.

175 oposed to involve interactions with Bronsted acidic residues in the secondary coordination sphere.

176 ltimeric assemblies that bind STIM1 and that acidic residues in the transmembrane (TM) and extracellu

177 on is sensitive to mutation of two conserved acidic residues in the transmembrane segments.

178 Estimation of pKa values of the acidic residues in the transport sites indicates that at

179 ows us to estimate the pK(a) values of seven acidic residues in the unfolded state of HEWL.

180 ts assemble through contacts among basic and acidic residues in their transmembrane domains to form t

181 Acidic residues in this linker are required for its full

182 The acidic residues in this region are highly conserved in H

183 Acidic residues in this region, which are conserved to c

184 Acidic residues in v3 complement basic residue 110 of th

185 Acidic residues, in contrast, allowed the seal to form a

186 Mutagenesis shows that there are several acidic residues, including E99 and E107 as well as D29 (

187 des with phosphorylated residues proximal to acidic residues, including glutamic acid, aspartic acid,

188 rity of the cases the average pKa shifts for acidic residues induced by the complex formation are neg

189 la enhanced enzyme CPT sensitivity, with the acidic residues inducing the greatest increase in drug s

190 s further demonstrated that a mutation of an acidic residue involved in Ca(2+) binding, E454K, preven

191 -89 and 132-167) with the negatively charged acidic residues involved in Ca(2+) binding are critical

192 s important for proteolysis and a cluster of acidic residues involved in sensing antimicrobial peptid

193 t chain of MHC class II, but the role of the acidic residue is unclear.

194 Chemical cross-linking of acidic residues is achieved using homobifunctional dihyd

195 In the presence of calcium, the binding of acidic residues is enhanced as they ligate the cation, w

196 tion of the hinge histidine with neighboring acidic residues is proposed to be responsible for its ro

197 One feature of this stretch of acidic residues is that it lies adjacent to a functional

198 oop (named for its four invariably conserved acidic residues) is critical for Cdc34 function both in

199 We show that Asp8, among six acidic residues, is involved in non-native, electrostati

200 In contrast, mutation of an acidic residue known to be at or near the quinol-binding

201 ryptic fragments and generally correspond to acidic residues located at either end of each transmembr

202 ere, we have probed the roles of clusters of acidic residues located downstream from the core SUMO mo

203 of their collagen stalks and surface exposed acidic residues located in CR1 CCP24 and/or CCP25.

204 upplied in part by surface-exposed conserved acidic residues located on helix 1 and strand 1 in the s

205 changes from 4.0 to 3.5 and identify the key acidic residue-lysine interactions responsible for the o

206 Acidic residues markedly increase the rate of DeltapH-dr

207 or proteins with regions containing multiple acidic residues may also be affected similarly.

208 Acidic residues may also be specifically accommodated in

209 iently assembled at pH <5.0, suggesting that acidic residues might be involved in the initial membran

210 allows binding of PEP-19 to CaM, whereas the acidic residues modify the nature of this interaction, a

211 Mutation of these acidic residues modulates the effects of putrescine.

212 trostatics that would result from placing an acidic residue near the scissile phosphate of the bound

213 prisingly neither this residue nor any other acidic residues near the enzymes active site were essent

214 Acidic residues near the first residue decrease substrat

215 These results suggest that acidic residues normally modulate access of RNAs to the

216 ace of human CTRC that interact with the P4' acidic residue of the inhibitor.

217 Mutations in key acidic residues of GreA and DksA suggest that properties

218 Arg82 and Arg132) on one side, and multiple acidic residues of L3 (Asp107, Asp113, Glu117, Asp121, A

219 e interfacial region modulate protonation of acidic residues of pHLIP responsible for transbilayer in

220 A mutant in which acidic residues of SP were replaced with alanine failed

221 bridges between basic residues of IL-13 and acidic residues of the antibody.

222 h the structure of the complex, in which the acidic residues of the C-terminal extension cannot inter

223 ely interacts with both of the transmembrane acidic residues of the CD3 dimer.

224 c residues on either side, which bond to the acidic residues of the finger.

225 e present evidence suggesting that the three acidic residues of the IN active site function through m

226 When the serine and acidic residues of the SRT were replaced with Ala, Leu,

227 Replacement of acidic residues on cyt c(6) that correspond to the PC's

228 Mutations of conserved basic or acidic residues on either face suppressed PsIAA4 PB1 hom

229 In this study, we have identified two acidic residues on helix-162 (Glu-167 and Glu-170) on th

230 equence-conserved surface patch encircled by acidic residues on one face of the protein.

231 sidues form electrostatic complexes with two acidic residues on the channel.

232 actin subdomains 1 and 3 juxtaposed against acidic residues on the successive quasi-repeating units

233 jacent immunoglobulin-like domain (Ig5), and acidic residues on the surface of FN1 play a role in pol

234 In addition, specific acidic residues on the surface of the MAM domain are cri

235 ods P2, P3, P5, and P7, where both basic and acidic residues or predominantly acidic residues contrib

236 rprisingly, MD2 does not interact with three acidic residues or the Mg(2+) cofactor required for cata

237 tide charge was changed by mutating basic to acidic residues or vice versa, but the amino acid sequen

238 ing to the TiO2 surface, with both basic and acidic residues overwhelmingly preferred over the non-ch

239 ic residues in the N-terminal alpha-helix to acidic residues partially or completely restored normal

240 al peptide activity and membrane disruption, acidic residues positioned at the distal (Asp(20), Asp(2

241 Interestingly, we found that LigY lacks the acidic residue proposed to activate water for hydrolysis

242 nteraction between a lysine and a cluster of acidic residues provides one prong with an arginine and

243 Alanine-scanning mutagenesis identified an acidic-residue putative ER export motif responsible for

244 receptor (SPRY) domain binds to a cluster of acidic residues, referred to as the D/E box, in RbBP5.

245 identical positioning of key hydrophobic and acidic residues relative to NSP3.

246 IKKbeta/NF-kappaB, whereas replacement with acidic residues rendered MEKK3 constitutively active.

247 ersely, Smo becomes constitutively active if acidic residues replace those phosphorylation sites.

248 lues may reflect binding interactions to the acidic residue-rich a1 and a3 segments adjacent to A1 do

249 We further demonstrate that an acidic residue-rich region in SRC-3 is an important dete

250 The structure reveals an acidic-residue-rich subdomain that is present in bocavir

251 A continuous axis of basic and acidic residues running centrally through the membrane a

252 fter ligand binding, a proton may bind to an acidic residue(s) in the transmembrane domain, i.e., Asp

253 found the interactions to depend strongly on acidic residues surrounding the central core LIR motifs.

254 ns, we present here the development of a new acidic residue-targeting sulfoxide-containing MS-cleavab

255 On the other hand, acidic residues tend to form "water bridges" between the

256 ficity helix." Additionally, in CANSDC a key acidic residue that interacts with the distal amino grou

257 rongly targeted, and mutagenesis revealed an acidic residue that is important for toxin binding.

258 dherins consisting of three highly conserved acidic residues that alternately serve as a p120-binding

259 ith an active site lined by highly conserved acidic residues that bind metal ions.

260 We have mutated all of the acidic residues that coordinate the Ca(2+) ions and have

261 he adaptable platform surface, and a trio of acidic residues that enables cation selectivity without

262 on in repression, the positions of basic and acidic residues that form inter-molecular salt bridges i

263 GNE-6640 and GNE-6776 interact with acidic residues that mediate hydrogen-bond interactions

264 The surface of Ocr is replete with acidic residues that mimic the phosphate backbone of DNA

265 handshake" interaction between two conserved acidic residues that our data suggest adds an additional

266 or is due to a network of interactions among acidic residues that would destabilize the alpha-helical

267 bind lipids and a C-terminal domain rich in acidic residues (the acidic tail).

268 of two regions of the ectodomain enriched in acidic residues: the acidic pocket, which faces the outs

269 ting systematic mutagenesis of all conserved acidic residues thought to be exposed to the cytoplasm,

270 This adds an acidic residue to the CcO interior.

271 VIII were assessed following mutagenesis of acidic residues to Ala or Tyr residues to Phe and expres

272 ctivators that target sigma(70) directly use acidic residues to engage a basic surface of region 4.

273 Mutations that changed acidic residues to neutral residues (e.g. E8A, D11S, E12

274 Replacement of these serine residues with acidic residues, to mimic phosphorylation, reduced prote

275 gion, which houses three histidines and four acidic residues, to Mn(II) coordination at site 2.

276 embrane MCPs where reversible methylation of acidic residues tunes receptor activity.

277 However, mutation of a single acidic residue upstream of the dileucine-like motif lead

278 le, most eukaryotic genomes, with respect to acidic residue usages, select Glu over Asp, but the oppo

279 The specificity of the two acidic residues was further demonstrated by changing the

280 Furthermore, the FTIR signal of protonated acidic residues was perturbed in the presence of Zn(2+),

281 d by the carboxylate of two highly conserved acidic residues, water molecules, and the 3'-hydroxyl gr

282 Because only this first NPF is followed by acidic residues, we have utilized glutathione S-transfer

283 ovel electrostatic palmitoyl switch in which acidic residues weaken the membrane-binding strength of

284 Conserved acidic residues were confirmed through kinetic analysis

285 In this method, the catalytic acidic residues were each substituted with cysteines.

286 us) partitioned into membranes only when its acidic residues were neutralized by protonation.

287 Some acidic residues were observed to orient and move signifi

288 of Ocr, termed pocr, in which all of the 34 acidic residues were substituted for a neutral amino aci

289 se organization of conserved hydrophobic and acidic residues when compared with canonical BH3 sequenc

290 tatic requirements: one surprisingly prefers acidic residues when driven by either a Deltapsi or a De

291 addition, it is also proposed that a pair of acidic residues, which are totally conserved among the C

292 alization; mutational replacement of several acidic residues with neutral or basic residues rescues P

293 proteins is due to independent titration of acidic residues with pK(a) values perturbed primarily by

294 ystokinin receptor interacts with a specific acidic residue within the amino terminus of this recepto

295 rization of these mutants, we also define an acidic residue within the C-terminus of cyclin D1 that i

296 ith steroidogenic factor-1 that involve four acidic residues within its ligand-binding domain, mutati

297 s studies on this enzyme, revealing that two acidic residues within the double E loop, Asp-147 and Gl

298 Mutation of basic amino acids to acidic residues within the polybasic domain results in i

299 The acidic residues within this region (Glu72, Asp74, Glu89,

300 al mutagenesis of each of the five conserved acidic residues within this region of the receptor in th