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1 phosphates via sample digestion (heating and acidifying).
2 sitol-3-kinase from these organelles as they acidify.
3 lifespan, but that daughter cell vacuoles re-acidify.
4 deficient NC cells remain immature but still acidify.
5 promoted by the failure of M1 phagosomes to acidify.
6 , failure to acquire LAMP-1 and inability to acidify.
7 rm activity, whereas neurons are observed to acidify.
8 S stained intensely for the vATPase and were acidified.
9 ely open, the cytosol of sour taste cells is acidified.
10 ocean impacts narrow as the ocean warms and acidifies.
11 ctrospray ionization (ESI) of ubiquitin from acidified (0.1%) aqueous solution produces abundant ubiq
12 fast UHPLC separation (5min separation using acidified 10% MeOH isocratic flow in a CORTECS C18 colum
13 long and 5 mm diameter) and then eluted with acidified 5 M NaCl solution directly into a sodium aceta
18 includes a prior protein precipitation with acidified acetonitrile for all samples and an additional
21 rsely, expressing ATP12A in CF mouse airways acidified airway surface liquid, impaired defenses, and
22 H(+)/K(+) adenosine triphosphatase (ATP12A) acidified airway surface liquid, which impaired airway h
25 tion domain) as the lumen of the endosome is acidified and 2) low pH, in conjunction with acidic lipi
29 ltasecA2 mutant of M. tuberculosis were more acidified and showed greater association with markers of
30 with phagocytosis because phagosomes become acidified and the average fluorescence lifetime of EGFP
34 limate change as oceans continue to warm and acidify and thermal anomalies grow in frequency and inte
35 whole blood was added to the sample chamber, acidified, and the HCN gas evolved was actively transfer
36 ria-containing vacuoles quickly (</= 15 min) acidify, and that this was coincident with greater virul
37 phagosomes in murine cftr(-/-) AMs failed to acidify, and the cells were deficient in bacterial killi
39 brils upon incubation at room temperature in acidified aqueous solution at peptide concentrations >10
40 ions is produced by nanoESI from a slightly acidified aqueous solution with the micron outer diamete
42 e intramitochondrial pH of astrocytes, which acidifies as a consequence of glutamate uptake, with the
43 orter of acidified mitochondria reveals that acidified axonal mitochondria originating from the retin
44 act with early and late endosomes and become acidified before the onset of phagosomal disruption.
46 experiments to determine whether exposure to acidified bile salts causes esophageal epithelial cells
48 ophageal squamous cell lines were exposed to acidified bile salts to evaluate their effects on cytoki
49 at addition of moderate amounts of pepsin to acidified bovine insulin at close to physiological tempe
51 nt with an experimental study in which a CO2-acidified brine was injected into a fracture in the Dupe
52 omography before and after exposure to CO(2)-acidified brine, allowing mineral phase and voidspace di
54 ed intradermally to nape or cheek skin, (ii) acidified buffer elevated intracellular calcium levels i
56 croglia also degrade fAbeta if lysosomes are acidified by an ammonia pulse-wash or by treatment with
57 a persistent ozonide in airway-lining fluids acidified by preexisting pathologies or inhaled particul
59 milieu into an endosome, whose contents then acidify, causing changes in the viral matrix protein (M1
61 ression of all IFITMs expanded vesicular and acidified compartments within cells, there were marked p
65 ariability found among samples maintained in acidified conditions relative to controls, suggests a po
66 communities maintained in or transplanted to acidified conditions, and altered community structure in
67 cyanobacterium Trichodesmium decreased under acidified conditions, notwithstanding a beneficial effec
71 y undergo maturation and become increasingly acidified, consistent with the formation of an autolysos
74 dissolution of calcite particles by flow of acidified desalinated water through a bed packed with mi
81 ecovery from acidification, due to decreased acidifying emissions and deposition, has led to an incre
82 V-ATPase activity are avirulent and fail to acidify endomembrane compartments, exhibiting pleiotropi
86 of cellular entry requires trafficking to an acidified endosome, which promotes translocation across
87 serving as a route of export of folates from acidified endosomes and (ii) provide a functional role f
88 dendritic cells (pDCs) detect viruses in the acidified endosomes by means of Toll-like receptors (TLR
89 diphtheria toxin catalytic domain (DTA) from acidified endosomes into the cytoplasm of eukaryotic cel
90 facilitates egress of the viral genome from acidified endosomes, and the L2/DNA complex accumulates
91 gests hosts may need to adapt not only to an acidified environment, but also to changes in their Symb
92 e also display enhanced expression of the SC acidifying enzyme, secretory phospholipase A2f (sPLA2f).
94 one concentration (%), time of extraction in acidified ethanolic solution (min) and ethanol concentra
99 E. ictaluri-containing vacuoles (ECV) became acidified following ingestion by head kidney-derived mac
104 nant protein and electron cryo-microscopy of acidified hantavirus allows us to propose a model for en
107 n 5days and then approximately pH 8.5) to be acidified in PLGA MS for 9weeks, unlike CM2-free PLGA MS
109 reasoned that the Salmonella cytoplasm might acidify in the macrophage vacuole to activate OmpR-depen
110 trongly diminish the ability of lysosomes to acidify in vivo, demonstrating that ClC-7 is a Cl-/H+ an
111 into the intestinal lumen and are lacking in acidified intestinal V-ATPase-containing compartments.
113 vesicular adenosine triphosphatase (ATPase) acidifies intracellular compartments, including synaptic
115 ase (V-ATPase) is a rotary motor enzyme that acidifies intracellular organelles and the extracellular
116 -ATPases are ATP-dependent proton pumps that acidify intracellular compartments and, in some cases, t
118 e) is a multisubunit complex responsible for acidifying intracellular organelles and is highly regula
122 ication of the host cell vacuole, Salmonella acidifies its own cytoplasm in response to the low extra
124 pace between the intestine and the muscle is acidified just prior to muscle contraction and that the
125 -524) is sufficient to pilot the effector to acidified LAMP1-positive lysosomal compartments, where W
128 could account for enhanced function, because acidifying lightly pigmented human SC resets barrier fun
129 mes via the TAT moiety and to respond to the acidifying lumen of endosomes to cause membrane leakage
131 compartment defects in resistance; V-ATPase acidifies lysosomes and related organelles, whereas adap
134 e CiaRH, ComDE, or VicRK grew more slowly in acidified media and were more sensitive to killing at le
135 ltaBbPacC mutant showed a reduced ability to acidify media during growth due to failure to produce ox
139 ic-lipophilic balanced resin and eluted with acidified methanol (0.1% formic acid), resulting in anal
142 udes cell lysis, protein precipitation using acidified methanol and HPLC analysis of the lysate.
144 om minced muscle samples involved the use of acidified methanol, ultrasound treatment and centrifugat
147 s commonly used for phenols, extraction with acidified methanol-water was chosen as the best to quant
148 y with its potent inhibitor HOE 642 not only acidified microglia, abolished the BK-triggered dynamic
149 Sample preparation improvements included acidified, microwave-accelerated, PNGase F N-glycan rele
150 ubjects were randomly assigned to receive an acidified milk drink containing >/=10(9) colony-forming
151 duced tandem fluorophore protein reporter of acidified mitochondria reveals that acidified axonal mit
153 feature of these key species in the reduced acidified molybdate solutions and to observe the templat
154 ncentrations increase considerably in gypsum-acidified must, although they fell markedly after fermen
155 nced precipitation was, however, observed in acidified NaHCO(3) solutions (pH 5, DIC approximately 25
157 cales and that as coastal oceans continue to acidify, negative effects on these populations may becom
158 ollowing topical application of the NO donor-acidified nitrite (NO2(-)), has set the paradigm of NO b
159 e nitrogen species produced biochemically as acidified nitrite and by bone marrow-derived macrophages
160 and a treatment arm that received 3 doses of acidified nitrite applied topically for 12 weeks with an
162 nitrosylation upon exposure of Salmonella to acidified nitrite, a signal encountered by this enteropa
164 gest that the potent inflammation induced by acidified NO2(-) is secondary to the release of addition
165 daptive capacity of species to respond to an acidified ocean, and, as a result, predictions regarding
166 ed to parental care are more protected in an acidifying ocean compared to their relatives employing b
169 egional weather patterns, rising sea levels, acidifying oceans, changed nutrient loads and altered oc
170 S stained intensely for the vATPase and were acidified, only 20 to 30% of phagosomes containing live
171 ke of anthropogenic carbon dioxide (CO2) has acidified open-ocean surface waters by 0.1 pH units sinc
172 ectrical current through the system tends to acidify (or basify) the anolyte (or catholyte), their ef
175 H(+)-ATPase (V-ATPase) enzyme complex is to acidify organelles; this process is critical for a varie
176 inetics in Cd-Zn cocontaminated alkaline and acidified paddy soils, under various flooding periods an
177 intracellular pathogen that replicates in an acidified parasitophorous vacuole derived from host lyso
178 n, the pathogen replicates exclusively in an acidified (pH 4.5 to 5) phagolysosome-like parasitophoro
179 gote stage of the parasite that lives within acidified phagolysomal vesicles of mammalian macrophages
185 iplasmic protease MarP for Mtb to survive in acidified phagosomes and establish and maintain infectio
187 ce are metabolically inactive, reside within acidified phagosomes that have fused with Texas red dext
188 ence are metabolically active, reside in non-acidified phagosomes that have not fused with Texas red
190 When compared with the control (pHi 7.3), acidifying pHi to 6.3 or alkalinizing pHi to 8.3 and 8.8
192 SA300 clone, the USA300-containing phagosome acidified rapidly and acquired the late endosome and lys
194 ires osteoclasts to generate and maintain an acidified resorption compartment between the apical memb
196 ve flow-through CO2 equilibration between an acidified sample and an indicator solution with a fast r
197 ve flow-through CO2 equilibration between an acidified sample and an indicator solution with a respon
198 ydrides were generated in the reaction of an acidified sample with NaBH4 after pre-reduction with KI-
199 reviewed experimental data on the effects of acidified seawater on calcifying species growth, reprodu
200 s of PNH erythrocytes in a modified extended acidified serum assay, and also prevents C3 fragment dep
201 opment, allowing serpulids to do best at the acidified site by the end of the experiment, although ea
203 pH(i) recovery was significantly greater in acidified slc4a10-transfected cells than in control cell
205 d to grow at higher rates in the presence of acidified sodium nitrite (ASN), a model system used to g
207 77-83% moisture content) were conditioned in acidified solution at 5, 15 and 25 degrees C, absence of
208 t of carbonate mineral reactivity with CO(2)-acidified solution is voidspace generation through physi
211 nvironmental problem, but efforts to restore acidified streams and biota have had limited success.
213 TPase) is a multisubunit enzyme complex that acidifies subcellular organelles and the extracellular s
214 ta vacuoles were alkalinized and the cytosol acidified, suggestive of impaired V-ATPase proton transp
215 This claim is based on the beliefs that (i) acidified sulfanilamide pretreatment, required to remove
216 ations were only enhanced in the presence of acidified sulfate aerosol, consistent with prior work.
217 Additionally, we show reactive uptake on the acidified sulfate aerosols supports a previously unrepor
218 ich form during the BVOC oxidation, into the acidified sulfate particles is shown to explain the latt
219 low-NO(x) SOA is enhanced in the presence of acidified sulfate seed aerosol (mass yield 28.6%) over t
222 Remarkably, low concentrations of glutamate acidify synaptic vesicles more slowly but to a greater e
223 These findings suggest the existence of an acidifying synaptic force that is overcome by commonly u
225 Inhibition of extramitochondrial CA activity acidified the matrix (as determined by fluorescence meas
229 The gastric proton pump H(+),K(+)-ATPase acidifies the gastric lumen, and thus its inhibitors, in
231 expresses vacuolar H(+)-ATPase (VHA), which acidifies the symbiosome space down to pH approximately
234 ctivating process in which proton permeation acidifies the virion to release the viral RNA and core p
235 sed, whereby auxin promotes proton efflux to acidify the apoplast and facilitate the uptake of solute
236 nzymes' catalytic power and their ability to acidify the C alpha of amino acids remains unknown.
239 ides, could grow faster at pH 5.5, and could acidify the environment more rapidly and to a greater ex
241 (V-ATPases) are essential proton pumps that acidify the lumen of subcellular organelles in all eukar
242 y-stimulating factor (MCSF) or interleukin-6 acidify the lysosomes of microglia and enable them to de
243 anthropogenic carbon dioxide (CO2) emissions acidify the oceans, calcifiers generally are expected to
248 hemical library to search for compounds that acidify the yeast cytosol in vivo using pHluorin-based f
249 effect of acidic medium could be mimicked by acidifying the cytosol with bafilomycin A1, a proton pum
250 cent muscle cells to contract by transiently acidifying the extracellular space between the intestine
252 ng in the activation of the O2' nucleophile, acidifying the general acid C75, and stabilizing the non
255 abnormalities were normalized by exogenously acidifying the SC, suggesting a basis for the well-known
256 bound uropathogenic E. coli and responded by acidifying the urine and secreting the bacteriostatic pr
257 east cells grown in abundant glucose tend to acidify their extracellular environment, they raise the
259 and 52.4% of p.E161K carriers were unable to acidify their urine below pH 5.3 and thus, can be consid
260 s of V-ATPase function; cells were unable to acidify their vacuoles and exhibited growth defects typi
261 /endosomal network and vacuolar membrane and acidified these compartments as part of a hybrid V-ATPas
262 mes, within a C. neoformans-containing cell, acidified to a lesser extent and failed to recruit CD63
265 total dissolved iron analysis, samples were acidified to pH 2.0 in the presence of 30 muM DHN and le
267 is can persist in macrophage phagosomes that acidify to a pH of approximately 4.5 after activation of
269 ed that without amelogenins the enamel would acidify unless ameloblasts were buffered by alternative
270 mobilization of metal contaminants by brine acidified upon contact with CO2 during geologic carbon s
271 ns remain regarding how bacteria survive the acidified vacuole and how acidification affects bacteria
272 of macrophages, where Brucella resides in an acidified vacuole at a pH of 4-4.5 during the early phas
274 virulence mechanisms differentially rely on acidified vacuoles and that the loss of both vacuolar (V
275 lar proton pump, blocked MDP accumulation in acidified vesicles and cytokine responses, suggesting th
276 c machinery to discriminate fully loaded and acidified vesicles from vesicles undergoing neurotransmi
278 ation, labeled MDP was rapidly released from acidified vesicles into the cytosol, a process that requ
279 et cells triggers toxin internalization into acidified vesicles, whereupon cryptic segments from with
282 The desolvated solutions were dispersed in acidified water (pH 3) immediately after desolvation.
285 ects of the extraction methods and solvents (acidified water pH 2.0, ethanol+water 50% v/v and ethano
286 ite electrode readily produces hydrogen from acidified water when functionalized with the modified cl
287 terials and with a diffusion-limited rate in acidified water, is efficient as well as oxygen tolerant
290 sistent spatial mosaic in the penetration of acidified waters into ecologically-important nearshore h
292 sion, vesicles deep in the egg center slowly acidify, whereas cortical vesicles undergo a rapid alkal
293 se the oceanic surface water to continuously acidify, which has multiple and profound impacts on coas
295 modified QuEChERS technique in acetonitrile acidified with acetic acid (1%, v/v) and citrate-buffere
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