ライフサイエンスコーパス: acini

1 n of EPI64 and EPI64B in isolated pancreatic acini.

2 repress HNF6 and are expressed in developing acini.

3 astoma cells and in three-dimensional MCF10A acini.

4 ls formed enlarged and poorly differentiated acini.

5 ltons [SNAP23], and VAMP2) in rat pancreatic acini.

6 t cohesively as they divide to assemble into acini.

7 apical polarity longitudinally for the same acini.

8 ng remained quiescent within growth-arrested acini.

9 on CCK-8-stimulated exocytosis in pancreatic acini.

10 rchitecture of silicon rubber casts of mouse acini.

11 to enlarged, disorganized, three-dimensional acini.

12 dent model of cell damage for salivary gland acini.

13 ease from lacrimal gland pieces but not from acini.

14 S results in lumen-filled mammary epithelial acini.

15 onents of this pathway in rat lacrimal gland acini.

16 pieces or digested with collagenase to form acini.

17 ignant cell lines compared with nonmalignant acini.

18 as has been observed in Her2/Neu-expressing acini.

19 uced by the epithelial cells of the prostate acini.

20 hile no significant changes were observed in acini.

21 ne, develop from single cells into polarized acini.

22 iciently than cells harvested from uninduced acini.

23 tic peptides and fibroblast growth factor on acini.

24 oked oscillatory Ca(2+) increases across the acini.

25 GEF III, were identified in mouse pancreatic acini.

26 ranching ducts ending in hollow, sphere-like acini.

27 s and accelerated lumen formation in mammary acini.

28 structs of these proteins into permeabilized acini.

29 is significantly reduced Ad5 transduction of acini.

30 tions of lymphocytes from periductal foci to acini.

31 and apoptosis during morphogenesis of MCF10A acini.

32 OS was present in the nerves surrounding the acini.

33 ymphatic vessels located close to and around acini.

34 ion and could not form or maintain polarized acini.

35 rotein secretion was increased in transduced acini.

36 gamma(-/-) acini but not in p110 gamma(+/-) acini.

37 rptive function of Na/K-ATPase in the type I acini.

38 n by suppressing fluid transport in type III acini.

39 ctures opening towards the lumen in type-III acini.

40 large, lobular structures rather than hollow acini.

41 body and extended to the apical parts of the acini.

42 etion and increased the number of functional acini.

43 hose previously observed in dispersed rodent acini.

44 disrupted ductal tree and a >90% deficit of acini.

45 d tubule formation and stunted the growth of acini.

46 and survival in the luminal space of mammary acini.

47 tis-causing treatments as observed in rodent acini.

48 hrough differentiation of the PanIN cells to acini, accompanied by re-expression of the acinar transc

49 tical role in the regeneration of pancreatic acini after resection.

50 The image-based segmentation of individual acini allowed the computation of acinar volume and surfa

51 111 reorganizes mammary cells into polarized acini, allowing both the exposure of the prolactin recep

52 etected in cells and in the apical region of acini along AQP5.

53 Human acini also secreted inflammatory mediators elevated in a

54 VAMP8(-/-) acini also showed a >90% decrease in the early endosomal

55 examined in rats (1) in vitro with isolated acini and (2) in vivo with an acid challenge.

56 were fully mechanically isolated from other acini and also from the bulk gel by box-cuts with a side

57 In isolated rat pancreatic acini and AR42J cells, we determined the effect of tumor

58 analysis revealed restriction of CEACAM20 to acini and CEACAM1 to tubule structures, respectively.

59 lrECM prevents formation of organized breast acini and disrupts growth arrest.

60 found differentially expressed in the serous acini and duct cells of all major salivary glands.

61 d PKA activation in both isolated pancreatic acini and duct fragments.

62 xpressed protein was localized to pancreatic acini and ductal cells.

63 C6 and AC9 were expressed in both pancreatic acini and ducts, whereas AC7 was expressed only in pancr

64 could be localized to the stroma surrounding acini and ducts.

65 eral AC isoforms are expressed in pancreatic acini and ducts.

66 posed of 2 secretory compartments, including acini and granular ducts connected by intercalated ducts

67 he stalk region) identities that produce the acini and higher order ducts, respectively.

68 c central neurons innervating salivary gland acini and identify different neuropeptides and their pre

69 of D52 followed by Rab5 and EEA1 in isolated acini and in in vivo The loss of D52 occurred as a conse

70 ed by high cyclin E both in cultured mammary acini and in mammary epithelial tissues in a mouse model

71 herent rotation is present in normal mammary acini and kidney cells but absent in cancerous cells.

72 polarity are essential for the formation of acini and link the functional relevance of CAMo to the e

73 ar effects to those found in Dicer-deficient acini and metaplastic cells, namely induction of HNF6 an

74 PI3K/AKT signaling, is overexpressed in the acini and PDAC of Pdx1-Cre;Kras(G12D/+);p53(f/+) (PKC) m

75 Pancreatic acini and peripheral blood mononuclear cells were expose

76 the disruption of architecture in epithelial acini and suggest that ERK1/2 can promote noninvasive mo

77 pressed in a subset of normal prostate gland acini and that Dkk-3 expression is reduced in prostate t

78 penton base, stimulated macropinocytosis in acini and that inhibition of macropinocytosis significan

79 l death responses in mouse and human primary acini and the acinar cell line AR42J.

80 cholinergic receptors in isolated pancreatic acini and the mechanisms responsible for other neurotran

81 of dynamic processes, e.g., the polarity of acini and the merging of polarized structures, upon tran

82 extracellular matrix (lrECM) form polarized acini and, in doing so, transit from a disorganized prol

83 ls, becoming highly prominent in peritumoral acini, and particularly high in acinar ductal metaplasia

84 prived cells in the luminal space of mammary acini, and the discovery that antioxidants facilitate th

85 as well as basolateral regions of ducts and acini; and (ii) OAG stimulated Ca2+ influx into disperse

86 cytosis processes in cultured rat pancreatic acini (apical blockade, basolateral exocytosis, and fusi

87 d debris (50.9%), gland dropout (42.8%), and acini appearance (54.5%).

88 ical outcomes ranged from fair agreement for acini appearance (kappa(w) = 0.23, 95% CI = 0.14-0.32) a

89 ading in a real-time examination compared to acini appearance and lid debris.

90 tion of partial glands in the lower lid, and acini appearance by the presence/absence of grape-like c

91 raphy grading of meibomian gland atrophy and acini appearance, and slit-lamp grading of lid debris an

92 Primary cultures of pancreatic acini appropriately responded to secretagogue stimulatio

93 3 zeta overexpression severely disrupted the acini architecture resulting in luminal filling.

94 Pancreatic acini are completely devoid of zymogen granules, and the

95 VAMP8(-/-) acini are resistant to secretory inhibition by supramaxi

96 ssion in single cells in organotypic mammary acini as a model to elucidate the processes by which onc

97 r expression is inhibited in Dicer-deficient acini, as well as in pancreatitis-induced metaplasia.

98 Interacting acini begin to disorganize within 12.5 +/- 4.7 h in a sp

99 As acini begin to form, PTEN accumulates both in the cytopl

100 t in G0-G1 and differentiated into polarized acini between days 5 and 7.

101 fibroblast growth factor) to induce mammary acini branching, indicative of a more invasive fibroblas

102 on of SOX2 results in a failure to establish acini but not ducts.

103 activation was inhibited in p110 gamma(-/-) acini but not in p110 gamma(+/-) acini.

104 sitive myoepithelial cells were found in the acini but not in the normal ducts or dacryops epithelium

105 construction and quantitative study of whole acini by image analysis and stereologic methods, yieldin

106 Inhibition of miRNA synthesis in acini by inactivation of Dicer and pancreatitis-induced

107 Thus, pairs or groups of mammary acini can interact mechanically over long distances thro

108 ial tissues such as kidney tubules or breast acini, cells organize into bidimensional monolayers expe

109 Acini coexpressing receptor and ligand exhibit a dramati

110 d is then highly upregulated in all cells of acini, coincident with detection of apoptosis in the cen

111 I-MEC) proliferated to produce new secretory acini composed of secretory luminal cells and myoepithel

112 xin to cleave VAMP2 in VAMP8 knock-out (-/-) acini confirmed that VAMP2 and -8 are the primary VAMPs

113 nesis to form a complex secretory organ with acini connected to an extensive ductal system.

114 tioning units (hepatic plates and pancreatic acini) connected to the ductal tree.

115 as was acinar metaplasia in which individual acini consisted of acinar cells and duct-like cells.

116 The resulting acini contained prominent central lumina not observed wh

117 In AR42J and primary acini, CSE+EtOH induced cell death (necrosis and apoptos

118 ne-treated primary three-dimensional mammary acini cultures.

119 found that cyclin E deregulation in mammary acini decreases, in an E2F-independent manner, expressio

120 The phenotype of Dicer-deficient acini depends on the induction of HNF6; overexpression o

121 ated invasiveness and disrupted formation of acini despite continued E-cad expression.

122 that do not interact mechanically with other acini disorganize more slowly (in 21.8 +/- 4.1 h) and to

123 factor deprivation, Cav-1-deficient mammary acini displayed increased ERalpha levels and enhanced se

124 ar morphogenesis, and induction in preformed acini disrupted the pre-established acinar architecture

125 Without PDX1, acini do not form; the precursor epithelium continues to

126 Using the acini-ductal network of the developing human and murine

127 Zymogen activation, observed within acini early during acute pancreatitis for a long time, w

128 Inducible activation of ERK1/2 in mature acini elicits cell motility and disrupts epithelial arch

129 When MYC and Kras(G12D) are induced, the acini enlarge and form solid, depolarized spheres.

130 polarized, growth-attenuated, multicellular acini, enveloped by a continuous endogenous BM.

131 titis, Munc18c-depleted (Munc18c(+/-)) mouse acini exhibited a reduction in pathological basolateral

132 As in rodent acini, human acini exposed to toxic concentrations of CCh and tauroli

133 Pancreatic acini express the complexin 2 isoform by RT-PCR and immu

134 In the presence of TN-C, however, acini failed to generate a normal BM, and net epithelial

135 Mammary epithelial acini features were compared using OP or EP under condit

136 e utilizing MCF10A mammary epithelial cells, acini form due to integrin-dependent polarization and su

137 The ectopic expression of Runx2 disrupts acini formation, and electron microscopic ultrastructura

138 premature growth arrest during mammary cell acini formation, whereas Amot130 (S175A)-expressing cell

139 Pancreatic ducts and acini from control mice and early-stage PanINs from KPC

140 Proteomic analysis of acini from donors of diverse ethnicity showed similar pr

141 27A, but dependent on Rab27B, as shown using acini from genetically modified mice.

142 Here, we found that pancreatic acini from Munc18c-depleted mice (Munc18c(+/-)) and huma

143 Acini from rat lacrimal gland were isolated by collagena

144 ed as image processing tools to discriminate acini from spheroids without any 3D reconstruction.

145 4D-live imaging of rotating MCF10A mammary acini further suggests an evolutionary conserved mechani

146 ve transcriptome analysis of human prostatic acini generated in a three-dimensional basement membrane

147 t Notch and ErbB1/2 both play a role in DCIS acini growth and stem cell activity.

148 The functions of these pathways in acini have been linked to mitogenesis, protein synthesis

149 As in rodent acini, human acini exposed to toxic concentrations of CC

150 nome-wide mRNA expression analysis of MCF10A acini identified 158 genes regulated by the mutant MYC a

151 ast cells leads to the formation of abnormal acini in 3D culture, but does not promote cell migration

152 : They exclude Hoechst dye 33342, and reform acini in 3D cultures and repopulate mammary fat pads mor

153 ed by the disruption of the morphogenesis of acini in a physiologically relevant three-dimensional mo

154 r in number and larger in size compared with acini in controls.

155 cell proliferation of mammary 3-dimensional acini in culture.

156 Further contrasting with EP, acini in OP displayed cooperation between ErbB2 signalli

157 uction and enriched the number of functional acini in submandibular glands of irradiated animals and

158 mage processing tools to separate individual acini in the mouse lung.

159 ously, we have shown that patients with >40% acini in the pancreatic transection line are most prone

160 neutrophils were largely absent around gland acini in the submucosa.

161 Growth of MCF-10A acini in three-dimensional (3D) culture was enhanced upo

162 n promotes a tumor cell phenotype of mammary acini in three-dimensional culture.

163 optosis in 14-3-3 zeta-overexpressing MCF10A acini in three-dimensional cultures.

164 F10A/p65 cells failed to form characteristic acini in three-dimensional Matrigel.

165 Furthermore, the acini in transgenic mice were fewer in number and larger

166 with hollow lumen similar to normal mammary acini in vivo.

167 ifferentiated mammary epithelial structures (acini) in three-dimensional culture triggers the loss of

168 2+]i was measured using an imaging system in acini incubated with fura-2/AM.

169 Analysis of VAMP8(-/-) acini indicated that although stimulated secretion was s

170 hyperproliferative and disorganized mammary acini induced by chronic stimulation of colony-stimulati

171 o mucous cells does not occur, nor are gland acini inflamed with neutrophils.

172 f recombinant complexin 2 into permeabilized acini inhibited Ca(2+)-stimulated secretion in a concent

173 these growth-arrested and polarized mammary acini initially led to reinitiation of cell proliferatio

174 antagonized the ability of Kras to reprogram acini into PDA preneoplastic precursors.

175 Suprastimulation of pancreatic acini is a well-known model for pancreatitis, and it is

176 is and find that Myc transgene expression in acini is much lower than in unorganized cells.

177 se that internalization of Ad5 into lacrimal acini is through a novel fiber-dependent mechanism that

178 iated by Na/K-ATPase in type III and type II acini, is followed by a dopamine-independent resorptive

179 esolve components in the pancreas, including acini, islets, blood vessels, and extracellular matrix.

180 formed ex vivo pancreatitis studies in human acini isolated from cadaveric pancreata from organ donor

181 sinogen activation were similarly reduced in acini isolated from p110 gamma(-/-) and p110 gamma(+/-)

182 activation were all diminished in pancreatic acini isolated from p110 gamma(-/-) mice.

183 r, conclusive evidence that human pancreatic acini lack functional CCK-A receptors has been presented

184 x (ECM), and the apoptosis of inner cells of acini lacking contact with the ECM.

185 n intensified the Vav2-induced disruption of acini, leading to more aggressive cell outgrowth and bra

186 ate glandular differentiation, we identified acini-like PCA and related molecular markers that signif

187 s (MCF-10A) form polarized, growth-arrested, acini-like structures with glandular architecture.

188 mers induced disruption of three-dimensional acini-like structures, only heterodimers promoted invasi

189 A human mammary epithelial cells form hollow acini-like structures, we have observed both caspase-med

190 expressing this profile, which we designated acini-like tumors, had a significantly lower risk of pos

191 roscopy subsequently revealed that polarized acini lipids were more ordered at the apical membranes c

192 imaging system, [Ca(2+)](i) was measured in acini loaded with fura-2.

193 resorptive function of Na/K-ATPase in type I acini located in the proximal end of the salivary duct.

194 In acini, lowering pHe from 7.6 to 6.8 enhanced secretagogu

195 In conclusion, human cadaveric pancreatic acini maintain physiological functions and have similar

196 anned, only the high-risk patients with >40% acini (n = 62) continued in the study to receive in tota

197 nitors that give rise to both new islets and acini normally after birth and after injury (ductal liga

198 In vitro experiments were performed in which acini obtained from wild-type and Gpbar1(-/-) mice were

199 bsent in normal mammary ducts, ductules, and acini of histologically normal breast and scanty in the

200 ducibly express active MLK3 in the preformed acini of MCF10A cells grown in 3D Matrigel.

201 Paired arrays from ducts and acini of the five animals were scanned and analyzed with

202 The ducts and acini of the human mammary gland are prototypical hetero

203 lammatory cell infiltration of the stroma or acini of the lacrimal glands and conjunctivae of both sa

204 l, we found that regression of the secretory acini of the mammary gland was compromised in the absenc

205 is study, the expression in mouse pancreatic acini of two candidate Tre-2/Bub2/Cdc16 (TBC) domain-con

206 amylase release were examined in pancreatic acini of wild type and Mist1 null mice to gain insight i

207 an immature stage, without the formation of acini or islets.

208 pherical or cylindrical cellular structures (acini or tubes).

209 reast cancer risk with increasing numbers of acini per lobule (P = .0004).

210 ast Disease Cohort, by determining number of acini per lobule and lobular area.

211 These differences in acini phenotype observed between OP and EP highlight the

212 lated rat hepatocyte couplets and pancreatic acini, plus SkHep1 cells as nonpolarized controls.

213 Human acini preferentially expressed the muscarinic acetylchol

214 eliance on rodent models employing dispersed acini preparations.

215 f mutant mice was hypoplastic and individual acini produced few zymogen granules.

216 olarity in live breast glandular structures (acini) produced in three-dimensional cell culture.

217 o-basal polarity in normal breast epithelial acini requires a balance between cell proliferation, cel

218 basement membrane around mature, nonrotating acini restored rotational movement and the ability to as

219 -related C3 botulinum substrate) in ILK-null acini restored the lactation defect, indicating that RAC

220 The reduced Ca(2+) influx in TRPC3(-/-) acini resulted in reduced frequency of the physiologic C

221 5 penton base, fiber, and knob with lacrimal acini revealed that the penton base capsid protein remai

222 ons between acini were cut with a laser, the acini reverted to a slowly disorganizing phenotype.

223 l progenitors forming a network of ducts and acini (secretory cells).

224 In WT acini, short term (14-16 h) culture also results in a >9

225 VAMP8(-/-) acini show increased expression of the endosomal protein

226 ro studies using freshly prepared pancreatic acini show that genetic deletion of PAR2 reduces bile sa

227 of Ca2+ signaling in wild-type and Trpc3-/- acini showed that Pyr3 is a highly specific inhibitor of

228 this study we demonstrate that DAPT reduced acini size and mammosphere formation in MCF10DCIS.com wh

229 Lapatinb reduced acini size and mammosphere formation in SUM225, whereas

230 nib treatment was more effective at reducing acini size in both DCIS cell lines.

231 Glandular tissues form ducts (tubes) and acini (spheres) in multicellular organisms.

232 Addition of fibronectin to differentiated acini stimulated proliferation and reversed growth arres

233 which can be studied in isolated pancreatic acini stimulated with supraphysiologic doses of cholecys

234 e mechanisms by which 14-3-3 zeta alters MEC acini structure and increases the risk of breast cancer.

235 nced proliferation and formation of aberrant acini structure in the three-dimensional culture.

236 Characterization of the disrupted acini structures showed increased cell proliferation (Ki

237 h in a spatially coordinated manner, whereas acini that do not interact mechanically with other acini

238 s, and that an inverse situation occurred in acini that lost apical polarity upon treatment with Ca(2

239 Here we use freshly isolated pancreatic acini that preserve the luminal structure to measure int

240 epithelial ductal networks that terminate in acini that together produce, transport and secrete saliv

241 qui parasites in the salivary gland granular acini, the parasites expressed levels of paralogous surf

242 specificity of targeting in 3D multicellular acini, these findings are promising and the approach mer

243 may inhibit anoikis and luminal clearance in acini through induction of autophagy.

244 ole for peripheral nerves in the creation of acini throughout development via regulation of SOX2.

245 Exposure of the acini to acetaldehyde and ethyl oleate followed by CCK-8

246 ress their 3D morphology from that of hollow acini to branched structures characteristic of nonmetast

247 Leung and Brugge use cultured breast cancer acini to demonstrate the importance of local interaction

248 Exposure of pancreatic acini to ethanol blocks cholecystokinin (CCK)-8-stimulat

249 Exposure of salivary gland cells and acini to hypotonicity elicited an increase in cell volum

250 ubnuclear targeting resulted in reversion of acini to more normal structures and reduced tumor growth

251 minating on specific cells of salivary gland acini (types II and III).

252 small interfering RNA (siRNA) into lacrimal acini under conditions that reduced intracellular CAR mR

253 of this secretory blockade, Munc18c-depleted acini unexpectedly activated a component of the endoplas

254 holecystokinin (CCK) was also seen in intact acini using immunofluorescence, in a biotinylated fracti

255 enchymal interdependence between neighboring acini was accounted for.

256 During the operation, the percentage of acini was calculated from pancreatic transection line fr

257 ced mucin secretion by rat sublingual tubulo-acini was dependent upon PLC activation and the subseque

258 list of 10,294 genes expressed in ducts and acini was searched using gene ontologies related to ion

259 lux of fluid through the epithelial cells of acini, we propose that complex control of the tick saliv

260 CCK-induced responses in p110 gamma(-/-) acini were all further inhibited by LY294002, indicating

261 Cultured Bmi1(-/-) and wild-type primary acini were analyzed in vitro to determine acinar-specifi

262 the directed mechanical connections between acini were cut with a laser, the acini reverted to a slo

263 When acini were fully mechanically isolated from other acini

264 Rat pancreatic acini were incubated for 1-4 hours with FAEEs or acetald

265 Acini were incubated with adenoviruses overnight or the

266 Rat pancreatic acini were incubated with concentrations of ethanol asso

267 Rat lacrimal gland acini were incubated with H89, an inhibitor of protein k

268 Acini were incubated with the fluorescence indicator fur

269 Acini were incubated with the fluorescent indicator mole

270 Lacrimal gland acini were isolated by collagenase digestion and incubat

271 Rat lacrimal gland acini were isolated by collagenase digestion, and protei

272 Rat lacrimal gland acini were isolated by collagenase digestion.

273 Rat lacrimal gland acini were isolated by collagenase digestion.

274 Primary acini were isolated from mice and analyzed in amylase se

275 Acini were preincubated with inhibitors for 20 minutes b

276 Acini were preincubated with the PKC inhibitors calphost

277 Acini were stimulated with the P2X(7) receptor agonist,

278 Acini were stimulated with the P2X7 receptor agonist, (b

279 gene expression was again seen when the HMEC acini were sub-cultured as a monolayer, implying that lr

280 Rat lacrimal gland acini were transduced with an adenovirus containing a ge

281 Permeabilized acini were used to understand the differential role of c

282 F10A cell line differentiates to form hollow acini when grown in Matrigel, and expression of LMP2A in

283 ng morphogenesis in human mammary epithelial acini when grown in three-dimensional cultures and are a

284 le adult epithelial cells generate polarized acini when placed in a surrogate basement membrane 3D ge

285 form polarized, growth-arrested structures (acini) when cultured in three-dimensional laminin-rich e

286 cytic foci and areas of altered or distorted acini, whereas the ID/Rx group had scattered small lymph

287 grin-null mice had higher numbers of mammary acini, which may account for the earlier onset of tumors

288 ct of GI hormones on c-Met in rat pancreatic acini, which possess both receptors.

289 ency factors (Sox9 and Hnf1beta) in Ptf1a(+) acini, which undergo rapid reprogramming to duct cells a

290 age-independent conditions and form aberrant acini, which was dependent on Ad-cyclin E or Ad-LMW-E ex

291 and other neoplastic-like changes in mammary acini, while silencing alphaB-crystallin by RNA interfer

292 f Ad5 with excess heparin or pretreatment of acini with a heparinase cocktail each inhibited Ad5 tran

293 Here we show that mammary acini with compromised structural integrity can intercon

294 sis in the luminal area, resulting in bigger acini with filled lumens.

295 rostate epithelial cells that form polarized acini with lumen under standard tridimensional (3D) cult

296 Pretreatment of acini with Src inhibitors or expression of a cortactin t

297 ls retained the capacity to generate mammary acini within extracellular matrix.

298 evelopment of hyperplastic three-dimensional acini without affecting apical-basal polarity.

299 aminin, and fibronectin levels in pancreatic acini without affecting messenger RNA (mRNA) expression

300 CF10A cells formed irregular and near-normal acini without hollow lumen in three-dimensional culture.