ライフサイエンスコーパス: acquire by

1 Images acquired by 3D profiling and SEM indicated that the surf

2 Ehrlichiae were acquired by 90%-100% of feeding larvae.

3 human brain cancer and from a silica phantom acquired by a 1310 nm swept-source OCT (SS-OCT) system.

4 suring the color from the image of the array acquired by a digital camera, using the H coordinate of

5 ed the functional contributions of mutations acquired by a human variable (VH) domain that was evolve

6 components automatically from digital images acquired by a low-cost platform.

7 d 1,089 haloarchaeal gene families that were acquired by a methanogenic recipient from eubacteria.

8 radic ALS and 10 control lumbar spinal cords acquired by a rapid autopsy system that processed nervou

9 l aspects of the complex expression patterns acquired by a receptor family that switched from sensing

10 of images followed by a third set of images acquired by a second observer.

11 her 3 scans centered on the macula that were acquired by a single operator.

12 f non-linear kinetic-spectrophotometric data acquired by a stopped-flow system for the quantitation o

13 and studied the ability of those genes to be acquired by Acinetobacter baylyi.

14 ginate from intrinsic lipid properties or be acquired by adsorption of peripheral molecules.

15 ovis, a reemerging threat to U.S. cattle, is acquired by adult female ticks of the subgenus Boophilus

16 Three companies, OptiMedica, LenSx (acquired by Alcon in September 2010), and LensAR, in dif

17 diversification of a single BCL2 family gene acquired by an ancestral poxvirus.

18 tentially in other alveolates, may have been acquired by an endosymbiosis of an early ochrophyte.

19 ) infection is that the pathogens are orally acquired by an insect vector during the taking of a bloo

20 fficient method for aggregating measurements acquired by an uncalibrated WDESN, and producing accurat

21 that evolve in one lineage are often stably acquired by another lineage.

22 ly from lymph in the subcapsular sinus to be acquired by antigen-specific B cells in the underlying f

23 be applied to high-resolution pill tracking acquired by any modality.

24 However, treated virions were not acquired by aphids through the hindgut epithelial cells

25 ation data from 13 aromatic side chains were acquired by applying canonical relaxation experiments to

26 NMR spectra were acquired by applying the robust, sensitive and rapid WET

27 Hippocampal volume was acquired by automated segmentation of magnetic resonance

28 We hypothesize that hemin is acquired by B. bronchiseptica in a BhuR-dependent manner

29 large quantities in the environment, can be acquired by bacteria through natural transformation.

30 have enabled the identification of mutations acquired by bacterial pathogens during infection.

31 h information from images that are routinely acquired by biologists, and provides insights - in parti

32 ring the previous year and the viruses newly acquired by birds during winter months spent in the sout

33 ssion is absent from early precursors and is acquired by bone marrow B cells newly expressing the BCR

34 Spectra were acquired by both AP MALDI and vacuum MALDI and directly

35 Although anal HPV infection is commonly acquired by both MSW and MSM, incident events and persis

36 indings suggest that fear-related plasticity acquired by brain regions outside of the BLA, unlike tho

37 The phenotype acquired by breast epithelial cells clearly implies that

38 Explanted airway mucosal biopsy specimens, acquired by bronchoscopy from subjects with asthma, were

39 In particular, SAR 2-5 appears to have been acquired by Brucella after it became intracellular.

40 s is ensured in part by a state of tolerance acquired by CD4 T cells to germline-encoded sequences wi

41 , we found that the differentiation programs acquired by CD8 T cells after brief or prolonged antigen

42 o the enhanced hydrophobicity of the surface acquired by cholesterol binding resulting in the folding

43 Moreover, mechanistic insights acquired by chromatin immunoprecipitation demonstrate th

44 pears to be an intrinsic functional property acquired by clonally diverse B cells.

45 tation pattern of the metallosupramolecules, acquired by collisionally activated dissociation after T

46 o lipid and central carbon metabolism can be acquired by combining RPLC ESI positive and HILIC ESI ne

47 Many learned motor behaviors are acquired by comparing ongoing behavior with an internal

48 ern recognition technique to analytical data acquired by comprehensive two-dimensional gas chromatogr

49 Images acquired by confocal scanning laser ophthalmoscopy (Spec

50 It is acquired by consumption of unpasteurized dairy products

51 eptospirosis is potentially a fatal zoonosis acquired by contact of skin and mucosal surfaces with so

52 these infections are foodborne, but many are acquired by contact with animals.

53 Changes in hemagglutinin (HA) acquired by contemporary A(H3N2) viruses hinder antigeni

54 cted from the curve of glucose concentration acquired by continuous glucose monitoring system (CGM);

55 ivity and specificity rates, which cannot be acquired by conventional CT and MRI methods, can be acqu

56 ndicate that certain motivational properties acquired by cues on the basis of appetitive learning inv

57 the MIPIN proteins in patients with MI were acquired by data mining of the PubMed and UniProt knowle

58 New fate is acquired by decommissioning old and establishing new sup

59 ed, and resistance to PARP inhibition can be acquired by deletion of a mutation in BRCA2.

60 ow that resistance to PARP inhibition can be acquired by deletion of a mutation in BRCA2.

61 , LTP-dependent spatial task that was easily acquired by delta-/- mice.

62 ILT)3 is crucial to the tolerogenic activity acquired by dendritic cells exposed to allospecific T su

63 riming, the presentation of protein antigens acquired by dendritic cells from their environment.

64 sify brain tumors based on lipid information acquired by desorption electrospray ionization mass spec

65 These data sets were acquired by different cameras, camera platforms and in v

66 Impedance, acquired by different lead electrodes, has variable resp

67 ury resistance Tn5042, appeared to have been acquired by different pQBR plasmids by recombination, in

68 d from their chemical fingerprint signatures acquired by direct analysis in real time high-resolution

69 eported through 30 May 2006, nearly all were acquired by direct contact with poultry.

70 hree Entamoeba species while ERE2 was likely acquired by E. histolytica after its separation from E.

71 ell as in stabilization of various positions acquired by each domain upon substrate binding and utili

72 , i.e. homologous genes that apparently were acquired by early eukaryotes via different routes, inclu

73 gful estimates of the mean Mn valence can be acquired by EELS if proper care is taken.

74 have indicated that PA-specific antibodies, acquired by either active or passive immunization, are s

75 ri infections are common and possibly can be acquired by either superinfection or coinfection.

76 ce fluxes that mimic the Aharonov-Bohm phase acquired by electrons.

77 ution mass spectra of natural organic matter acquired by electrospray ionization Fourier transform io

78 Similar mutations probably need to be acquired by emerging influenza A viruses before they can

79 or computed tomographic images that had been acquired by employee health services or by the employee'

80 udies have demonstrated that small specimens acquired by endoscopic ultrasound transbronchial needle

81 Subsequently, enhanced Vif function is acquired by enforced mutations.

82 HCEs were acquired by enzymatic digestion of cadaveric conjunctiva

83 The mass spectra acquired by ESI FT-ICR MS of untreated, borohydride-redu

84 copies, implying that each fimbrial type was acquired by ETEC strains very recently, consistent with

85 ggests that these toxin deaminases have been acquired by eukaryotes on several independent occasions

86 NCB data acquired by experiments at pH 6.6 without any preliminar

87 formal, symbolic maths abilities that humans acquire by explicit instruction remains unknown.

88 early learning process, in which targets are acquired by exploration and reinforcement rather than se

89 Postnatal CMV infection acquired by exposure to raw maternal milk is very freque

90 Such spirochetes were acquired by feeding ticks and migrated to the tick saliv

91 mmunofluorescence analysis of B. burgdorferi acquired by feeding ticks from the blood of infected mic

92 s reflected in the population of spirochetes acquired by feeding ticks.

93 al reproduction, male-borne symbionts can be acquired by females and subsequently transferred to sexu

94 omer in each of these organic modifiers were acquired by frontal analysis over a 20 000 dynamic conce

95 Adsorption isotherm data of caffeine were acquired by frontal analysis.

96 ansmission measurements with protein spectra acquired by FT-IR spectroscopy.

97 r other types of data, including 3D datasets acquired by functional magnetic resonance imaging and EM

98 ether, our data suggest that Ag itself, when acquired by gammadelta T cells, directs the specificity

99 ns of prioritizing sample processing of data acquired by GCxGC-TOF MS for the identification of poten

100 Surprisingly, these isolates had acquired by gene conversion the complete gonococcal deni

101 ulin-like sequences that appear to have been acquired by Gram-negative bacteria from their metazoan h

102 estimates the distribution of new infections acquired by group based on these factors within a Bayesi

103 Heme is acquired by H. ducreyi from its human host via TonB-depe

104 analysis suggested that cysK-metB-luxS were acquired by H. pylori from a Gram-positive source.

105 Surprisingly, epitope antigenicity acquired by HA trimerization persists following acid tri

106 of Archaea have one or more protein domains acquired by HGT, and nearly 30-50% of Bacteria did the s

107 The proteoform profile of C9 was acquired by high-resolution native mass spectrometry, wh

108 l adaptive mechanism of apoptosis resistance acquired by HIV-infected cells to avoid their eliminatio

109 had a pathoadaptive variant of fimH that was acquired by homologous replacement into the commensal st

110 ncrease in the pan-genome frequency of genes acquired by horizontal gene exchange.

111 Genomic data indicate that the cassette was acquired by horizontal gene transfer and possibly locate

112 ifficult to determine whether the intein was acquired by horizontal gene transfer or was vertically i

113 ther suggested that pirAB(vp) may be lost or acquired by horizontal gene transfer via transposition o

114 lases but not in some bacterial orthologues (acquired by horizontal gene transfer), which has implica

115 s from pharyngeal glands, some of which were acquired by horizontal gene transfer, to manipulate host

116 , including ring-splitting dioxygenase genes acquired by horizontal gene transfer.

117 ctivity of glycosyltransferase (gtr) operons acquired by horizontal gene transfer.

118 ethanolamine and propanediol was apparently acquired by horizontal gene transfer.

119 ed that the nrdIEF cluster of B. hermsii was acquired by horizontal gene transfer.

120 tions in different strains, with many likely acquired by horizontal gene transfer.

121 ly as a silencer of AT-rich genetic material acquired by horizontal gene transfer.

122 rreconcilable with the current view of genes acquired by horizontal transfer as being accessory or ad

123 ontent of these two genes suggests they were acquired by horizontal transfer events.

124 evidence that key virulence genes have been acquired by horizontal transfer from other eukaryotic an

125 pancies to identify bacterial genes recently acquired by horizontal transfer.

126 ggest that the yydFGHIJ operon may have been acquired by horizontal transfer.

127 action with the host and which may have been acquired by horizontal transfer.

128 must infect the female germ line after being acquired by horizontal transfer.

129 ions by applying a novel algorithm to images acquired by hot stage microscopy.

130 e that autoantigens and exogenous lipids are acquired by human CD1d at distinct cellular locations, a

131 (CD16a) is encoded by the FCGR3A gene and is acquired by human NK cells during maturation.

132 ess by which antibiotic resistance genes are acquired by human pathogens, we functionally characteriz

133 e among the most common bacterial infections acquired by humans, particularly in catheterized patient

134 inant enzymes from Plasmodium parasites were acquired by incrementally heating them to 90 degrees C a

135 hat the three archaeal types of Locus I were acquired by independent events of lateral gene transfer.

136 ainly by Brucella melitensis, which is often acquired by ingesting contaminated goat or sheep milk an

137 M. avium is commonly acquired by ingestion, and a large number of AIDS patien

138 Since it can be acquired by inhalation and is difficult to eradicate due

139 most hepatitis C virus (HCV) infections are acquired by injection drug use, prospective data on the

140 had ever attended the screening or not) was acquired by inspecting the well documented screening rec

141 Heme can also be acquired by intestinal absorption and intercellular tran

142 to the T-cell immunological synapse in data acquired by iPALM providing 10 nm isotropic resolution.

143 that infectious mononucleosis is most likely acquired by kissing.

144 ice when it is injected intradermally and is acquired by larval ticks fed on these mice, where it per

145 when introduced by needle inoculation, (ii) acquired by larval ticks feeding on infected mice, and (

146 ns of alkylchlorocarbenes (RCCl) are readily acquired by laser flash photolysis with UV-vis detection

147 Prostate epithelium was acquired by laser-capture microdissection, and transcrip

148 ike protein coding sequences might have been acquired by lateral gene transfer and the number of heme

149 ysis indicated that all three genes had been acquired by lateral gene transfer from the low-moles-per

150 P. woesei may have acquired by lateral gene transfer more than 100 ORFs fro

151 y islands from other bacteria and are likely acquired by lateral gene transfer.

152 ls that induce cell-type specialization were acquired by lateral gene transfer.

153 +) -driven MotAB stator, the latter possibly acquired by lateral gene transfer.

154 and SufB proteins of prokaryotes that it has acquired by lateral transfer from an archaeon related to

155 hput identification of phospholipids in data acquired by LC-MS and shotgun experiments.

156 that ratios of selected archaeal tetraethers acquired by LDI FTICR-MS are highly correlated with valu

157 Thus the LigN protein acquired by LGT appears to have been co-opted as a back-

158 ed on histogram analysis of raw axial images acquired by light-sheet fluorescent imaging (LSFI) to es

159 demonstrate that sexual identity in maize is acquired by limiting floral growth through negative regu

160 Data is acquired by MALDI multiple reaction monitoring (MRM) mas

161 It is commonly acquired by mammals such as rodents and humans via the b

162 artery plasma as an input function, usually acquired by manual blood sampling.

163 d by algae, fungi, and cyanobacteria and are acquired by many aquatic animals through trophic interac

164 Movement was acquired by means of an Optical Localization System and

165 istance and suggests that the resistance was acquired by means of horizontal gene transfer with the c

166 that >20% of the LB400 sequence was recently acquired by means of lateral gene transfer.

167 Three-dimensional photoacoustic images were acquired by mechanically scanning the US transducer and

168 ession of a unique set of "homing" receptors acquired by memory T cells.

169 Thus, new genes acquired by microbial genomes, on average, appear to be

170 trometry data of the respective peptides are acquired by microcapillary HPLC on an LTQ-orbitrap mass

171 tients shows that rtM204I/V is independently acquired by more than one intra-host HBV variant, indica

172 rons and pyramidal cells were simultaneously acquired by multiple neighboring electrodes of the Neuro

173 mechanisms that were conserved, rather than acquired, by mutations.

174 We show that this receptor is acquired by mycobacteria-containing phagosomes via inter

175 Finally, the F-actin curvature acquired by myosin-induced stresses can be further const

176 Using nuclear spectroscopy data acquired by NASA's Dawn mission, we determined the conce

177 unique, cell-autonomous migratory properties acquired by neural crest cells when they become specifie

178 substrate specificity, serves as a mechanism acquired by neurons to achieve more precise control of g

179 Microbiota acquired by normal bees were abundant but quantitatively

180 the presence of cross-protective antibodies acquired by older individuals during previous exposures

181 ons of plants and animals themselves or were acquired by one or both lineages through horizontal gene

182 n.IMPORTANCE Many natural prion diseases are acquired by oral consumption of contaminated food or pas

183 Similar virulence plasmids have been acquired by other B. cereus strains and enable the patho

184 The ability to express the toxin has been acquired by other Escherichia coli strains, and outbreak

185 cted ticks into mammals until they are later acquired by other feeding ticks.

186 ibe for the first time an adaptive mechanism acquired by ovarian cancer cells that allows them to pro

187 tance during cancer chemotherapy is commonly acquired by overexpression of the ATP binding cassette t

188 P15, with mature sensitivity being gradually acquired by P18.

189 ion result in higher chances of a gene being acquired by Pack-MULEs.

190 s circulate in the hemolymph, where they are acquired by parasitic wasps during oviposition and subse

191 g metabolic functions have been horizontally acquired by pathogens to provide a selective advantage i

192 g that occurs due to the genomic aberrations acquired by patient tumors determines cellular behavior

193 the coexistence of highly divergent lineages acquired by patient-to-patient transmission.

194 of fundamental forms of appetitive learning acquired by Pavlovian associative processes.

195 lusive chemotherapeutic resistance mechanism acquired by PDA cells that reside in hypoxic PDA microen

196 A total of 21 of the 23 cases were acquired by person-to-person transmission in hemodialysi

197 is approach is based on the 3D surface model acquired by photogrammetry and fundamental parameter cal

198 oved by treating N as a resource that can be acquired by plants in exchange for energy, with differen

199 rive by subverting the resistance mechanisms acquired by plants to combat other pathogens.

200 opy images with high spatial resolution were acquired by post-processing a series of images obtained

201 In cross-priming, exogenous Ag is acquired by professional APCs, such as dendritic cells (

202 Analysis of >2 million images, acquired by quantitative fluorescence microscopy, showed

203 er per cell was within 17.5% of measurements acquired by quantitative RT-PCR.

204 Foods triggering PFS were acquired by questionnaire.

205 ages of ingredients of mixtures from spectra acquired by quite different methods such as X-ray photoe

206 e associative basis of Pavlovian fear memory acquired by rats with BLA lesions is similar to that of

207 age that has circulated in swine since being acquired by reassortment with seasonal human H3N2 viruse

208 ter is the only backbone region convincingly acquired by recombination as O157 emerged from O55.

209 t character" is a negative sensory attribute acquired by red wines when contaminating Dekkera/Brettan

210 Safety data were acquired by review of adverse events at study visits.

211 Here, we show that 70% of the overall Pi acquired by rice (Oryza sativa) is delivered via the sym

212 lation spectroscopy approach, where data are acquired by scanning each frame once and then scanning t

213 hondrion, cytosol, and apicoplast, a plastid acquired by secondary endosymbiosis of an alga.

214 GCFP images were acquired by selectively exciting the abdominal aorta and

215 Typically, three-dimensional (3D) images are acquired by sequentially capturing a series of 2D sectio

216 Skills acquired by simulation-based training seem to be transfe

217 ate unique diffraction patterns which can be acquired by smartphones and processed by a remote server

218 ely protein-surface intermolecular distances acquired by solid-state NMR.

219 the role of potential N-glycosylation motifs acquired by somatic hypermutation (ac-Nglycs) within Ig

220 epididymis, suggesting that this protein is acquired by sperm later during epididymal maturation.

221 tide-pulsed or virus-infected DCs are indeed acquired by splenic CD8alpha(-) DCs in vivo.

222 sponding full retinal thickness measurements acquired by Stratus OCT3.

223 ssues, we show that TGFBR1*6A is somatically acquired by stromal and epithelial cells adjacent to col

224 g two-dimensional and three-dimensional data acquired by structured illumination microscopy (SIM).

225 Contact hypersensitivity responses were acquired by such mice after adoptive transfer of natural

226 rance via sulfide oxidation already had been acquired by sulfidogenic wall-less archaebacteria (therm

227 ation and teamwork in the competencies to be acquired by surgical residents before graduation.

228 M. abscessus was thought to be independently acquired by susceptible individuals from the environment

229 resent an ancestral female-specific function acquired by Sxl in an early evolutionary step toward its

230 ignals and tissue-selective homing receptors acquired by T cells during activation and differentiatio

231 yte antigen G (HLA-G) were antigens commonly acquired by T cells from malignant plasma cells.

232 Furthermore, the requisite experience was acquired by the anesthetized brain and in the absence of

233 o, genes supporting photosynthesis have been acquired by the animal via horizontal gene transfer and

234 terized the gain functions for all resources acquired by the Batek at daily timescales and examined h

235 maternal mitochondria, whereas mitochondria acquired by the bud are largely free of aggregates.

236 d that four of these core gene families were acquired by the Ca.

237 of M. pneumoniae-positive primary specimens acquired by the CDC was performed using MLVA.

238 Using images acquired by the Chang'e-2 spacecraft, more than 200 boul

239 teria accounted for most (> 70%) of the iron acquired by the colony, highlighting the importance of d

240 es examined are evolutionarily ancient (were acquired by the common ancestor of simians).

241 d by conventional CT and MRI methods, can be acquired by the CTP method.

242 ved during pyrolysis of two mudstone samples acquired by the Curiosity rover at Yellowknife Bay withi

243 us (HCMV) is the most common viral infection acquired by the developing human fetus and can result in

244 The method was developed on data acquired by the DIA method SWATH-MS and benchmarked usin

245 sites, cross-links, or miscoding lesions are acquired by the environmental bacterium Acinetobacter ba

246 anges within a Mars year, detected in images acquired by the High-Resolution Imaging Science Experime

247 However, images acquired by the High-Resolution Imaging Science Experime

248 In images acquired by the Imaging Science Subsystem (ISS), this re

249 Magnetometer data acquired by the MESSENGER spacecraft in orbit about Merc

250 notated human ARPs with high accuracy and is acquired by the most common familial melanoma-associated

251 eed, Spc72 (the receptor for the gammaTC) is acquired by the new SPB outer plaque partway through spi

252 Cytomegalovirus (CMV) is acquired by the oral route in children, and primary infe

253 d by the TnSmu2 operon, which appeared to be acquired by the organism via horizontal gene transfer.

254 Images of comet 67P/Churyumov-Gerasimenko acquired by the OSIRIS (Optical, Spectroscopic and Infra

255 t protein that anchors the surface fibers is acquired by the passage of capsids through a membrane en

256 ron is reduced by the specific polymorphisms acquired by the PfCRT variant as a result of chloroquine

257 uces severe, acute, often fatal disease when acquired by the respiratory route.

258 ched HSCT, where tolerance to self is either acquired by the stem cell-derived NK cell after exiting

259 hree slices (more commonly four or five) are acquired by the subsequent dimension resulting in both c

260 that R. montanensis presumably invades once acquired by the tick.

261 uct CO(2) using MIMS are comparable to those acquired by the traditional endpoint assay based on the

262 Nevertheless, this ability could be acquired by the transfer of a larger segment of uPARAP/E

263 e two main antiviral drugs is differentially acquired by the two distinct subsets of neuraminidase as

264 e restriction by tetherin, this activity was acquired by the Vpu protein of HIV-1 group M viruses due

265 rom endogenous retroviruses (ERVs) that were acquired by the wild mouse progenitors of laboratory mic

266 Data amounts acquired by this system are very huge, and existing peak

267 cally, a large fraction of cases of STIs are acquired by those with modest risk, and these cases coul

268 Evidence acquired by three different methods indicates that the c

269 ble of establishing infection in mice, being acquired by ticks, persisting through tick molting, and

270 on anterior chamber angle (ACA) measurements acquired by time-domain anterior segment optical coheren

271 r composition to density profiles previously acquired by traditional ultrastructural methods, and pro

272 tes, screening US images will most likely be acquired by trained technologists rather than physicians

273 We document that mtDNA is acquired by transfer of whole mitochondria from the host

274 Metastatic traits seem to be acquired by transformed cells with progenitor-like cance

275 he prevalence of NoVs as a cause of diarrhea acquired by travelers in developing countries is not wel

276 ) uptake is modest as little additional N is acquired by trees; however, several correlations of ambi

277 We hypothesize that DXPas34 was acquired by Tsix to regulate antisense function.

278 contributes to the various phenotypic traits acquired by tumours as they progress and metastasise, an

279 nalgesic and hyperalgesic responses could be acquired by unseen (subliminally presented) stimuli.

280 e, 37 years +/- 8 [standard deviation]) were acquired by using 1.5- and 3.0-T MR imagers from three d

281 healthy control subjects were prospectively acquired by using 3.0-T magnetic resonance (MR) imaging

282 ra of single quantum states of pentenyne are acquired by using a combination of IR-Fourier transform

283 Each study was acquired by using a dual-detector camera, with each dete

284 All images were acquired by using a field of view of 3.6 x 3.6 x 2.0 cm.

285 data used as input were computed from images acquired by using a mixed fast spin-echo pulse sequence

286 d radiation dose, as each DBT projection was acquired by using about 22% of the dose for a single mam

287 histopathologic images to in vivo MR images acquired by using an endorectal receive coil was suffici

288 (standard deviation) and 32 +/- 6 for images acquired by using breathing protocols A and B, respectiv

289 copic signatures of breast lesions on images acquired by using diffuse optical spectroscopy imaging i

290 Coronary CT angiographic data were acquired by using dual-source CT.

291 An additional set of images was acquired by using real-time sequences with free breathin

292 that covered the entire left ventricle were acquired by using simultaneous multisection (SMS) excita

293 All data were acquired by using Somatom Definition.

294 acquired by using the routine dose and those acquired by using the one-tenth dose with HYPR-LR proces

295 an plots showed agreement between the images acquired by using the routine dose and those acquired by

296 Radiology CT quality assurance phantom were acquired by using three commercial CT systems at seven d

297 a (TD) is the most common infectious illness acquired by visitors to developing nations.

298 SNAT1 antagonism was acquired by Vpu variants from the lineage of SIVcpz/HIV-

299 Sodium currents were acquired by whole cell recording on HEK-293 cells transi

300 using simulated microscopy images and images acquired by widefield microscopy, total internal reflect