ライフサイエンスコーパス: actin filament

1 pendent on the direction of forces along the actin filament.

2 very of new actin subunits to the elongating actin filament.

3 proteins form end-to-end polymers along the actin filament.

4 mation communicated at the barbed end of the actin filament.

5 s processively toward the pointed end of the actin filament.

6 at fail to enter filopodia and co-align with actin filaments.

7 organization of the regulatory proteins and actin filaments.

8 ned that Fhod binds the sides of and bundles actin filaments.

9 rases that accelerate elongation of uncapped actin filaments.

10 e unusual, permitting only short, unstable F-actin filaments.

11 gulates the activation of product release by actin filaments.

12 actor assemble only one, stationary, zone of actin filaments.

13 ting effects of 2,4-D on the organization of actin filaments.

14 s inside domains without directly binding to actin filaments.

15 6(1/2) similar copies of the motif and binds actin filaments.

16 ances the bending and twisting compliance of actin filaments.

17 ing it is dependent on both microtubules and actin filaments.

18 viral replication compartment is assisted by actin filaments.

19 in domains consist of widely spaced parallel actin filaments.

20 hibition of myosin motors, and disruption of actin filaments.

21 the actomyosin network to mechanically align actin filaments.

22 roles of cofilin in severing and stabilizing actin filaments.

23 ing the inherent instability of apicomplexan actin filaments.

24 lymerize phalloidin- or jasplakinolide-bound actin filaments.

25 ter paths to the plasma membrane mediated by actin filaments.

26 M proteins in linking the plasma membrane to actin filaments.

27 proper length and organization of sarcomeric actin filaments.

28 cities and takes larger steps than on single actin filaments.

29 ich dynamically anchors the ER membrane with actin filaments.

30 activity and the availability of surrounding actin filaments.

31 ng and spreading cooperatively on individual actin filaments.

32 Arp2/3 complex to assemble a second zone of actin filaments.

33 protein involved in the depolymerization of actin filaments.

34 s a 37-residue repeating motif that binds to actin filaments.

35 ly tagged protein complexes along filopodial actin filaments.

36 the cell's three-dimensional (3D) highway of actin filaments.

37 Arp2/3 complex, which assembles two zones of actin filaments.

38 myosin known to move toward the minus end of actin filaments.

39 an important player in the polymerization of actin filaments.

40 eins that nucleate and processively elongate actin filaments.

41 and subsequently the organization of radial actin filaments.

42 egulate the severing and depolymerization of actin filaments.

43 tropomyosin (Tpm), which are associated with actin filaments.

44 imultaneous binding of both ABDs to separate actin filaments.

45 osin Vc (MyoVc) was not processive on single-actin filaments [1-3].

46 driven by Arp2/3-mediated polymerization of actin filaments [1].

47 ( 36 nm), similar to the step size on single actin filaments ( 38 nm).

48 ted a direct attachment of the plant ER with actin filaments [7, 8], but it is plausible that yet-unk

49 is mediated by reorganisation of guard cell actin filaments, a process that is finely tuned by the c

50 isms, and their cooperative association with actin filaments affects their ability to compete.

51 gel are due to continuous polymerization of actin filaments against the membrane surfaces of the ape

52 They are triggered by assembly of actin filaments along axon shafts giving rise to filopod

53 he network subsequently gradually reoriented actin filaments along the cell equator.

54 contractile actomyosin network consisting of actin filaments, alpha-actinin cross-linking proteins, a

55 However, actin filaments also align via search-and-capture, and t

56 h the inside-out activation of integrins and actin filament anchoring and thus plays a major role in

57 ing the number of heads that can bind to the actin filament and undergo a powerstroke once the cardia

58 the complex actin cytoskeleton, a network of actin filaments and actin bundles.

59 activates host gelsolin, leading to severed actin filaments and disturbed actin dynamics.

60 tate cancer cell lines and co-localizes with actin filaments and dynamic microtubules in filopodia of

61 , we found that tau colocalizes with MTs and actin filaments and is also located at the interface bet

62 ies of the conjugates not only interact with actin filaments and microtubules but also affect lipid r

63 from the other cytoskeletal filament types, actin filaments and microtubules, is their highly hierar

64 ns to demonstrate that if average lengths of actin filaments and myosin clusters are similar, then th

65 In muscle cells, actin filaments and myosin II appear in a polarized stru

66 pansion phase results in parallel bundles of actin filaments and myosin motors, generating progressiv

67 ws are generated by cross-linked networks of actin filaments and myosin motors, in which active stres

68 associated actin-binding proteins to dynamic actin filaments and myosin motors.

69 inesis are driven by a cortical ring rich in actin filaments and nonmuscle myosin II.

70 Nucleation Promoting Factor (NPF) proteins, actin filaments and NPF-recruited actin monomers.

71 in vivo, resulted in the loss of contractile actin filaments and perturbed epithelial packing geometr

72 , fingerlike structures that contain bundled actin filaments and project from the cell periphery.

73 osphorylation increases the turnover rate of actin filaments and promotes the short-term dynamics of

74 led to a dramatic reorganization of cortical actin filaments and the formation of actin-rich filopodi

75 nding the structural design and evolution of actin filaments and their function in motility and host

76 actin-severing protein gelsolin, to disrupt actin filaments and thus impair phagocytosis.

77 leton-associated protein that interacts with actin filaments and tubulin.

78 intestine with its prominent localization to actin filaments and was required for maintenance of inte

79 ing clathrin, clathrin-interacting proteins, actin filaments, and actin binding proteins, in a highly

80 ransport: myosin Va-dependent movement along actin filaments, and an unexpected vesicle hitchhiking m

81 e described, in particular, amyloid fibrils, actin filaments, and microtubules.

82 At saturation, only a fraction Drp1 binds actin filaments, and the off-rate of actin-bound Drp1 is

83 is required for pathogen-induced changes to actin filament architecture and host disease symptom dev

84 ns of dissipative biological systems such as actin filaments are a formidable scientific challenge in

85 Specifically, thin films of actin filaments are assembled at an oil-water interface.

86 Junction-associated actin filaments are dynamic structures that undergo cons

87 Actin filaments are mechanically coupled into a tight bu

88 Nevertheless, it remains elusive how actin filaments are reoriented, buckled, and bundled as

89 esented here show that both microtubules and actin filaments are responsible for mobility of nucleoca

90 As actin filaments are subject to complex mechanical constr

91 unappreciated reorganization of pre-existing actin filaments around WPBs before fusion, dependent on

92 ys a central role in nucleating the branched actin filament arrays that drive cell migration, endocyt

93 , processively attaches to the barbed end of actin filaments as a dimer and slows their elongation ra

94 site of endocytosis and initiates a zone of actin filaments assembled by Arp2/3 complex.

95 embly reactions, VopL/F exclusively nucleate actin filament assemblies, remaining only briefly associ

96 Actin filament assembly and disassembly are vital for ce

97 se proteins regulate almost all steps of the actin filament assembly and disassembly cycles, as well

98 nases but excluded phosphatases and enhanced actin filament assembly by recruiting and organizing act

99 a small actin-binding protein that inhibits actin filament assembly by sequestering actin monomers a

100 In yeast, Arp2/3-mediated actin filament assembly drives endocytic membrane invagi

101 Actin filament assembly in plants is a dynamic process,

102 ysia cell adhesion molecule (apCAM) leads to actin filament assembly near nascent adhesion sites.

103 p)/actin related protein (Arp) 2/3-dependent actin filament assembly.

104 be that, during actomyosin ring contraction, actin filaments associated with actomyosin rings are exp

105 ts a 30-40% lowered flexural rigidity of the actin filaments at [MgATP] </= 0.1 mM and local bending

106 B contained dense accumulations of branched actin filaments at approximately 50% of neurite tips at

107 We detect actin filaments at nuclear envelope rupture sites and de

108 controllable motors walk processively along actin filaments at speeds of 10-20 nm s(-1).

109 or the upstream role of ezrin in stabilizing actin filaments at the edges of TEMs, thereby favouring

110 cells, myosin-II binds and exerts force upon actin filaments at the leading edge, where clutching for

111 e, we quantify the dynamical organization of actin filaments at the onset of ring assembly in the C.

112 udopodia by virtue of its ability to cap the actin filament barbed end, which promotes Arp2/3-depende

113 y of capping protein (CP), a major capper of actin filament barbed ends in cells.

114 We find that the N-terminal ABD1 blocks actin filament barbed-end elongation, whereas ABD2 and A

115 bind to one another and together accelerate actin filament barbed-end elongation.

116 BP) terms, actin cytoskeleton organization, actin filament-based process, and protein ubiquitination

117 on the dynamic formation and disassembly of actin filament-based structures, including lamellipodia,

118 offset patterns of cortical microtubules and actin filaments between adjacent cells.

119 s fimbrin's N-terminal domain, and modulates actin filament binding to regulate actin cable assembly

120 Numerical simulations unified the roles of actin filament branching and crosslinking during actomyo

121 , highly directed manner along the polarized actin filament bundle within the filopodium becoming con

122 fusion by the porous structure of filopodial actin filament bundle, we used a particle-based stochast

123 and is also located at the interface between actin filament bundles and dynamic MTs in filopodia, sug

124 ulator, playing a pivotal role in organizing actin filament bundles at the ES.

125 Actin cables, composed of actin filament bundles nucleated by formins, mediate int

126 actin/thin filaments and developed abnormal actin filament bundles within sarcomeres that interconne

127 onflicting results on myosin-10 selection of actin filament bundles, demonstrating the importance of

128 m extending into growth cone filopodia along actin filament bundles.

129 Darkness induced changes in the extent of actin filament bundling in WT.

130 auxin not only controls the organization of actin filaments but also impacts vacuolar morphogenesis

131 city of other formins to nucleate and bundle actin filaments but is notably less effective at process

132 t VopL/F bind the barbed and pointed ends of actin filaments but only nucleate new filaments from the

133 enriched in exceptionally short and dynamic actin filaments, but the studies so far have not reveale

134 Nucleation of branched actin filaments by Arp2/3 complex is tightly regulated t

135 dinates the stochastic dynamic properties of actin filaments by modulating formin-mediated actin nucl

136 ly inhibited upon the capture and pulling of actin filaments by myosin, a result that has broad impli

137 at co-ordination of dynamic microtubules and actin filaments by the drebrin/EB3 pathway drives prosta

138 years as we try to understand the many ways actin filaments can take different flavors and unveil ho

139 In the MPS, short actin filaments, capped by actin-capping proteins, form

140 ealed that myosins and formins that nucleate actin filaments colocalize in plasma membrane-anchored c

141 Polymerization dynamics of actin filaments, comprising the structural core of filop

142 with E-cadherin that associates with radial actin filaments connecting cells over multiple layers.

143 is characteristic of myosin-X may facilitate actin filament convergence for filopodia production.

144 We found that the same actin filament crosslinkers either enhance or inhibit th

145 are particularly susceptible to compromised actin filament crosslinking activity.

146 Here we show that the highly conserved actin filament crosslinking protein fimbrin is a critica

147 tworks, and measure the resulting stress and actin filament deformations.

148 mponent organization, negative regulation of actin filament depolymerization and negative regulation

149 We previously showed that Drp1 binds actin filaments directly, and actin polymerization is ne

150 nto non-polymerizable complexes and enhances actin filament disassembly by severing, which is modulat

151 brane capacitance measurements, we show that actin filament disruption increases exocytosis in IHCs a

152 We discuss different factors known to make actin filaments distinguishable by regulatory proteins a

153 a development, modified lymphoma morphology, actin filament distribution, and migration properties of

154 t with appreciable structural changes in the actin filament during actomyosin-based motion generation

155 Although the reorganisation of actin filaments during stomatal closure is documented, t

156 ng of how they mechanistically contribute to actin filament dynamics has been limited.

157 ay bind to acidic phospholipids and regulate actin filament dynamics.Microbial pathogens secrete effe

158 uctures in which talin mediates a linkage to actin filaments either directly or indirectly by recruit

159 Furthermore, computer simulations of initial actin filament elongation and alignment revealed that an

160 ults identify CRMP-1 as a novel regulator of actin filament elongation and reveal a surprisingly impo

161 omoting actin nucleation and by accelerating actin filament elongation together with profilin [2].

162 blished a kinetic model of Ena/VASP-mediated actin filament elongation.

163 homology 1 domain and impede formin-mediated actin filament elongation.

164 P-170 binds tightly to formins to accelerate actin filament elongation.

165 ber of events in which different parts of an actin filament followed different paths.

166 Formins polymerize actin filaments for the cytokinetic contractile ring.

167 Actin filaments form different polymer networks with ver

168 ociated kinase pathway with increased stress actin filament formation in the EC layer.

169 Local actin filament formation powers the development of the s

170 ve mechanism, independently of their role in actin filament formation.

171 Polarized assembly of actin filaments forms the basis of actin-based motility

172 GJA1-20k protects actin filament from latrunculin A disruption, preserving

173 ilia widening by preventing newly elongating actin filaments from depolymerizing.

174 cations for perturbing tropomyosin-dependent actin filament function in the context of anti-cancer dr

175 Lamellipodia are networks of actin filaments generated and turned over by filament br

176 atic view based on an altered myosin-induced actin filament gliding pattern in an in vitro motility a

177 As the formin-actin filament has been shown to be part of an asymmetry

178 Actin filaments have key roles in cell motility but are

179 actin polymerization by stretching a single-actin filament in the absence of profilin using magnetic

180 The ability of Drp1 to bind actin filaments in a highly dynamic manner provides pote

181 ted by myosin pulling on barbed-end-anchored actin filaments in a stochastic sliding-filament mechani

182 ns(4,5)P2 in fluid-lipid bilayers can propel actin filaments in an unloaded motility assay, its abili

183 th cone periphery and close MT apposition to actin filaments in filopodia.

184 hirsutum) actin gene in the organization of actin filaments in lobed cotyledon pavement cells and th

185 product of myo2-E1-Sup1 does not translocate actin filaments in motility assays in vitro.

186 The role of actin filaments in nucleocapsid mobility was also confir

187 opn1sw1, GNB3 and PRPH2), and disruption of actin filaments in photoreceptors.

188 Actin filaments in plant cells are incredibly dynamic; t

189 serve Cy3-Cof1 and Cy3-Cof2 interacting with actin filaments in real time during severing.

190 eveal that disruption of smooth muscle alpha-actin filaments in smooth muscle cells increases reactiv

191 We further demonstrated that actin filaments in the entotic as well as invading cells

192 forms via actomyosin-driven condensation of actin filaments in the lamellipodia of migrating cells a

193 ssitated by the dynamic nature of Plasmodium actin filaments in the parasite.

194 M10(Full)LZ moves on actin filaments in the presence of PI(3,4,5)P3, an activ

195 her weak nucleator but efficiently elongates actin filaments in the presence of profilin.

196 Here we demonstrate a liquid phase of actin filaments in the presence of the physiological cro

197 d its ability to dynamically bind and bundle actin filaments in vitro using a combination of bulk sed

198 rthermore, disruption of smooth muscle alpha-actin filaments in wild-type smooth muscle cells by vari

199 and associates with the fast-growing end of actin filaments, influencing filament growth together wi

200 nsor, whereby myosin pulling on formin-bound actin filaments inhibits Cdc12-mediated actin assembly.

201 hway, which co-ordinates dynamic microtubule/actin filament interactions underlying cell shape change

202 d-bound cargo that encounters a suspended 3D actin filament intersection in vitro.

203 At actin filament intersections, the intersecting filament

204 Here, we were able to identify actin filament intersections, which likely correspond to

205 sembly cycles, as well as the arrangement of actin filaments into diverse three-dimensional structure

206 on of actin-associated proteins can organize actin filaments into dynamic patterns, such as vortices,

207 Filamin was shown to bind and cross-link actin filaments into higher-order structures and contrib

208 Cells organize actin filaments into higher-order structures by regulati

209 Filamin condenses short actin filaments into spindle-shaped droplets, or tactoid

210 erved proteins that non-covalently crosslink actin filaments into tight bundles.

211 this time, Ca(2+) -induced activation of the actin filament is maximal, while the myosin filament is

212 Assembling end-to-end along the actin filament is thereby not sufficient for tropomyosin

213 rigidity of heavy meromyosin (HMM)-propelled actin filaments is similar (without phalloidin) or sligh

214 n activation and subsequent interaction with actin filaments, it is likely that in its absence, contr

215 sly with beta- or gamma-catenin and cortical actin filaments, Kindlin-2 stabilizes adherens junctions

216 in Prosapip1 levels promote the formation of actin filaments, leading to changes in dendritic spine m

217 For this process, actin filament length and stability are temporally and s

218 ll culture assays, we identified HSPB7 as an actin filament length regulator that repressed actin pol

219 ork disruption increases exocytosis and that actin filaments may spatially organize a subfraction of

220 le in investigating the molecular origins of actin filament mechanical properties and modulation by t

221 sed motion generation, and modulation of the actin filament mechanical properties by the dominating c

222 d find that Myo2 pulling on Cdc12-associated actin filaments mechano-inhibits Cdc12-mediated assembly

223 e, we develop mesoscopic length-scale (cofil)actin filament models and evaluate the effects of compre

224 uption increases exocytosis in IHCs and that actin filaments most likely position a fraction of vesic

225 actin-activated ATPase activity and in vitro actin filament motility.

226 model that enables simulation of networks of actin filaments, myosin motors, and cross-linking protei

227 in expression promotes reorganization of the actin filament network and consequent mitochondrial dysf

228 Therefore, the actin filament network does not push directly toward the

229 in formation of a relatively stable cortical actin filament network resistant to cytochalasin D that

230 ing protein alpha-actinin SpAin1 bundles the actin filament network.

231 owth, were also found to have less disrupted actin filament networks after 2,4-D exposure.

232 n-binding proteins properly sort to distinct actin filament networks in the first place is not nearly

233 In particular, Listeria uses Arp2/3-mediated actin filament nucleation at the bacterial surface to ge

234 Competing models have been proposed for actin filament nucleation by the bacterial proteins VopL

235 We find that a spontaneous actin filament nucleation mechanism is required for adeq

236 The formin-family actin filament nucleator FMN2 associates with and genera

237 Leiomodins (Lmods) are a family of actin filament nucleators related to tropomodulins (Tmod

238 s using fluorescence spectroscopy and single actin filament observation in total internal reflection

239 We found that, while SpAin1 bundles actin filaments of mixed polarity like other alpha-actin

240 assay to investigate effects of rearranging actin filaments on the lateral membrane organization by

241 lso measured the motility characteristics of actin filaments on the model surfaces, i.e., velocity, s

242 We identify cycling of actin filaments onto and off of subsets of cellular mito

243 e disrupted and an increase in diameter when actin filaments or myosin II are disrupted.

244 We quantified actin filament order in human cells using fluorescence p

245 erein, we quantified spatial changes in host actin filament organization after infection with Pseudom

246 results suggest that the effects of 2,4-D on actin filament organization and root growth are mediated

247 hat the distinct effects of 2,4-D and IAA on actin filament organization partly dictate the different

248 verexpressing miR-1 have profound defects in actin filament organization that are partially rescued b

249 nct actin cytoskeleton networks with various actin filament organizations and dynamics through the co

250 When [MgATP] was reduced to </=0.1 mM, the actin filament paths in the in vitro motility assay beca

251 characterize the degree of meandering of the actin filament paths suggest that for [MgATP] >/= 0.25 m

252 of nucleocapsids in the cytoplasm, but that actin filaments play a larger role than microtubules in

253 e tension generated by the E-cadherin/AmotL2/actin filaments plays a crucial role in developmental pr

254 m with cross-linkers, can produce a range of actin filament polarity distributions and alignment, whi

255 ellicle and is influenced by the kinetics of actin filament polymerization and cytoplasmic calcium.

256 The molecular mechanism by which actin filament polymerization deforms the cell membrane

257 Inhibition of actin filament polymerization prevented the transport of

258 ells rely on specific tropomyosin-containing actin filament populations for growth and survival.

259 with specific tropomyosin isoforms generates actin filament populations with distinct functional prop

260 At high concentration, AtFH14 bundles actin filaments randomly into antiparallel or parallel s

261 er, the phase boundary allowed myosin driven actin filament rearrangements to actively move individua

262 all molecule inhibitor of drebrin binding to actin filaments, reduced the invasion of prostate cancer

263 ir pairwise and collective interactions with actin filaments regulate their activity and segregation

264 gidity, initial orientation, and turnover of actin filaments regulates the morphological transformati

265 A) obstructs phagocytosis through disrupting actin filament regulation processes - inhibiting polymer

266 To dissect out the role of tropomyosin in actin filament regulation we use the small molecule TR10

267 s, revealing the anisotropic distribution of actin filaments relative to the local retrograde flow of

268 The addition of actin filaments reorganized membrane domains.

269 Force toward the pointed (-) end of the actin filament resulted in a bond that was maximally sta

270 the rate of actin filament turnover and the actin filament's resulting super-diffusive behavior in t

271 actin binding affinities, and quantified the actin filament severing activities of human Cof1, Cof2,

272 turnover of cellular actin networks requires actin filament severing by actin-depolymerizing factor (

273 remodeling of the actin cytoskeleton and on actin filament severing by the regulatory protein cofili

274 uring cytokinesis is typically attributed to actin filaments sliding toward each other via Myosin-2 m

275 erization requires torsionally unconstrained actin filament, suggesting that mDia1 also senses torque

276 n almost 50% lower rate of dissociation from actin filament than NM-IIA and -IIC1 as determined by FR

277 geting drugs suggest that PMS contains short actin filaments that are depolymerization resistant and

278 rotein) family proteins to nucleate branched actin filaments that are important for cellular motility

279 tro and in vivo data, whereby Lmods nucleate actin filaments that are subsequently capped by Tmods du

280 ically decreased and that a subpopulation of actin filaments that assemble at high rates was reduced.

281 ontractile ring is a network of cross-linked actin filaments that facilitates cytokinesis in dividing

282 0(1-979)HMM are widely distributed on single actin filaments that is consistent with electron microsc

283 The complexes triggered rapid growth of actin filaments that remained attached to the MT surface

284 homodimers that bind with the barbed end of actin filaments through a ring-like structure assembled

285 a recent demonstration that pathogens target actin filaments to block plant defense and immunity.

286 highly dynamic manner provides potential for actin filaments to serve as reservoirs of oligomerizatio

287 n and modulates the cytoskeleton and tethers actin filaments to the Z-line of the sarcomere in muscle

288 The NMII motors then bind and pull actin filaments toward the NMII-F, thus driving contract

289 which is crucially dependent on the rate of actin filament turnover and the actin filament's resulti

290 Actin filament turnover underpins several processes in t

291 sliding between the myosin filament and the actin filament under zero load, V0 ) is already set at t

292 sliding between the myosin filament and the actin filament under zero load, V0 ) is already set at t

293 anism of fiber contraction by the sliding of actin filament upon myosin leading to conformational cha

294 g force of the myosin propagates through the actin filament, which behaves as an entropic spring, and

295 ell processes rely on specific assemblies of actin filaments, which are all constructed from nearly i

296 nally translated GJA1-20k isoform stabilizes actin filaments, which guides growth trajectories of the

297 experiments was dependent on the activity of actin filaments with little if any effect on inhibition

298 The association of actin filaments with mitochondrial subpopulations is tra

299 Cytoskeletal structures characterized by actin filaments with uniform lengths, including the thin

300 le enough to processively steps on different actin filaments within the actin bundles of filopodia.