ライフサイエンスコーパス: activase

1 antibody, and tissue plasminogen activator (Activase).

2 h activase but was well activated by tobacco activase.

3 acilitated activation by spinach and tobacco activase.

4 oenzyme that interacts directly with Rubisco activase.

5 ctivase was much more effective than tobacco activase.

6 l structure of the C-domain core of creosote activase.

7 e, the first structures of an AdoMet radical activase.

8 logous single and double mutations in cotton activase.

9 sco mutants, mimicking activation by spinach activase.

10 ter than tobacco Rubisco, similar to spinach activase.

11 her than for Antarctic hairgrass and spinach activase.

12 of CbbX, the alpha-proteobacterial red-type activase.

13 educing the activation of Rubisco by Rubisco activase.

14 imilar in function to pyruvate formate-lyase activase.

15 activase but now fully activated by tobacco activase.

16 n state of Rubisco via inhibition of Rubisco activase.

17 dynein motor proteins, TorsinA, and Rubisco activase.

18 co) in vivo requires the presence of Rubisco activase, a nuclear-encoded chloroplast protein that con

19 the Sensor 2 domain of the tobacco chimeric activase abolished its ability to better activate spinac

20 In contrast, tobacco chimeric activase activated spinach Rubisco far better than tobac

21 pecificity from non-Solanaceae to Solanaceae activase activation.

22 Thus, loss of activase activity during heat stress is caused by an exc

23 faster rate of deactivation, but by reduced activase activity possibly in response to unfavorable AT

24 etion of the first 50 amino acids of Rubisco activase almost completely eliminated the ability to act

25 ) complexes requires the activity of Rubisco activase, an AAA+ ATPase that utilizes chemo-mechanical

26 hat anaerobic ribonucleotide reductase (RNR) activase, an ancient enzyme that, it has been suggested,

27 n Arabidopsis clone (R100) with 30 to 40% Wt activase, an effect that was more pronounced in high lig

28 To examine interactions between Rubisco activase and ATP, site-directed mutations were made at t

29 res similar to those that denatured isolated activase and far below those required to denature Rubisc

30 egrees C, whereas the activities of isolated activase and Rubisco were highest at 42 degrees C and >5

31 l insights into the interactions between the activase and the G(734) loop of pyruvate formate-lyase a

32 roplastic (Rubisco small subunit and Rubisco activase) and peroxisomal (catalase) components of the p

33 nthetic enzymes, such as Rubisco and Rubisco activase, and enzymes of Suc biosynthesis such as Suc-ph

34 bisphosphate carboxylase/oxygenase (Rubisco) activase Arabidopsis thaliana with reduced concentration

35 nstrates for the first time that radical SAM activases are also capable of an alternative cleavage pa

36 d to date, there are two isoforms of Rubisco activase arising from alternative splicing that differ o

37 efine the size and function of this putative activase binding region, substitutions were created at p

38 isco was only partially activated by spinach activase but now fully activated by tobacco activase.

39 t, P89R Rubisco was not activated by spinach activase but was well activated by tobacco activase.

40 ability to regulate the activity of Rubisco activase by redox changes in the stroma.

41 ts into the mechanism of redox regulation of activase by the C-extension in the large isoform.

42 Activase content was 5% and 1.4% of total soluble protei

43 Tobacco activase does not markedly facilitate the activation of

44 The tobacco activase double mutant D311K/L314V activated wild type C

45 , K48- and K63-linked ubiquitination, and E1 activases, E2 conjugases, and E3 ligases involved in ubi

46 structural information for different Rubisco activase enzymes, the arrangement of these subunits in s

47 Isolated activase exhibited a temperature optimum for ATP hydroly

48 Rubisco activase facilitates this spontaneous carbamylation/meta

49 lamydomonas reinhardtii), and non-Solanaceae activase fails to activate Solanaceae Rubisco.

50 redoxin or ribulose-bisphosphate carboxylase activase for stromal targeting.

51 ive specificities of the spinach and tobacco activases for Chlamydomonas Rubisco were switched by cha

52 a variety of techniques to show that Rubisco activase forms a wide range of structures in solution, r

53 The data support a model in which Rubisco activase forms an open spiraling structure rather than a

54 Activase from Solanaceae plants (e.g. tobacco) fails to

55 hanging a Sensor 2-containing region between activases from spinach and tobacco.

56 To determine if loss of activase function is caused by protein denaturation, the

57 the maximal activity of the enzyme, Rubisco activase has an oligomeric state of 2-4 subunits.

58 at-treated activase trapped partially folded activase in an insoluble complex at treatment temperatur

59 of the N-terminal region of tobacco Rubisco activase in ATP hydrolysis and ribulose-1,5-bisphosphate

60 that Wt Arabidopsis does not contain Rubisco activase in great excess of the amount required for opti

61 These results support a role for activase in limiting photosynthesis at high temperature.

62 ccurred at 45 degrees C and 37 degrees C for activase in the presence and absence of ATPgammaS, respe

63 eriments showed that thermal denaturation of activase in vivo occurred at temperatures similar to tho

64 identified a substrate recognition region in activase in which two residues may directly interact wit

65 Rubisco activase is a nuclear-encoded chloroplast protein that i

66 Rubisco activase is an AAA(+) protein, a superfamily with member

67 Rubisco activase is an essential enzyme for photosynthesis, whic

68 Thus, the N-terminus of Rubisco activase is distinct from the ATP-hydrolyzing domain and

69 To determine whether the analogous domain of activase is involved in recognition of ribulose-1,5-bisp

70 Activase is thought to play a central role in coordinati

71 e that the C-extension of the oxidized large activase isoform can be cross-linked with regions contai

72 to redox regulate the activity of the larger activase isoform was eliminated by replacement of the cr

73 In the absence of activase, isolated Rubisco deactivated under catalytic c

74 uces oligomeric transitions to the bacterial activase, it merely enhances the kinetics of ATP hydroly

75 ohydrate distribution and influences Rubisco activase levels.

76 enetic basis for the function of the Rubisco activase-like gene (rca) was sought.

77 lomerata express Dgrca (D. glomerata Rubisco activase) mRNA, a transcript usually associated with pho

78 The ability of the two tobacco activase mutants to activate wild type and mutant Chlamy

79 KEY MESSAGE: Rubisco activase of plants evolved in a stepwise manner without

80 not D94K Rubisco, whereas the tobacco L314V activase only activated D94K Rubisco.

81 erefore, we speculate that upon binding, the activase paddles embrace the Rubisco cylinder by placing

82 The activase performs this remarkable feat by using an iron-

83 was associated with the expression of a new activase polypeptide.

84 posite mutation, D311K, in wild type tobacco activase produced an enzyme that activated both spinach

85 dation also modulated the activity of native activase proteins isolated from either Arabidopsis or sp

86 The Rubisco activase (RCA) gene from each species was sequenced.

87 ed the mechanism of the AAA+ protein Rubisco activase (Rca) in metabolic repair of the photosynthetic

88 bisphosphate carboxylase/oxygenase (Rubisco) activase (RCA) is organ-specific, light-responsive, and

89 eactivation due to the inhibition of Rubisco activase (RCA) under moderately elevated temperatures.

90 TP-synthase gamma-subunit (AtpC) and Rubisco activase (RCA) were identified by matrix-assisted laser-

91 Rubisco's catalytic chaperone, Rubisco activase (Rca), uses the energy from ATP hydrolysis to r

92 remodeled by the molecular chaperone Rubisco activase (Rca).

93 ested this method on the AAA+ ATPase Rubisco activase (Rca).

94 omonas reinhardtii mutant that lacks Rubisco activase (Rca).

95 rs are released by the motor protein Rubisco activase (Rca).

96 iverse molecular chaperones known as rubisco activases (Rcas).

97 Tobacco D311K activase readily activated wild type and P89R but not D9

98 in plants containing both isoforms, Rubisco activase regulates the activity of Rubisco in response t

99 psis thaliana with reduced concentrations of activase relative to wild-type (Wt) plants were measured

100 We propose a model in which Rubisco activase requires at least 1 neighboring subunit for hyd

101 The proteins Rubisco activase, Rubisco, and the 33-kD O2-evolving complex sub

102 In an attempt to eliminate the activase/Rubisco interaction, proline 89 was changed to

103 Increasing the activase/Rubisco ratio reduced Rubisco deactivation at h

104 This substitution also caused reversal of activase specificity, indicating that amino acid identit

105 for the pronounced cooperativity of Rubisco activase subunits, we suggest that coordination of the A

106 was estimated at 20 Rubisco active sites per activase tetramer in Wt Arabidopsis and 60 to 80 in the

107 results are consistent with a mechanism for activase that involves ATP-binding, subunit aggregation

108 sis, oxidation of the large (46-kDa) isoform activase to form a disulfide bond in the C-terminal exte

109 The ability of activase to maintain or promote Rubisco activation in vi

110 s been attributed to an inability of Rubisco activase to maintain Rubisco in an active form.

111 Rubisco deactivation exceeds the capacity of activase to promote activation.

112 nt tissue-type plasminogen activator (rt-PA, Activase) to methionine oxidation when treated with the

113 In Salix, Rubisco activase transcripts were down-regulated in contaminated

114 emically denatured rhodanese to heat-treated activase trapped partially folded activase in an insolub

115 Ubiquitin E1 activase UBA (ubiquitin activating enzyme)-6 and E3 liga

116 hizophrenia, with decreased levels of the E1 activase UBA3 and the E3 ligase Rnf7.

117 bisphosphate carboxylase/oxygenase (Rubisco) activase uses the energy from ATP hydrolysis to remove t

118 combinant creosote bush, cotton, and tobacco activase was 8 degrees C to 10 degrees C higher than for

119 Spinach chimeric activase was a poor activator of both spinach and tobacc

120 The stoichiometry of Rubisco to activase was estimated at 20 Rubisco active sites per ac

121 otein denaturation, the thermal stability of activase was examined in vitro and in vivo and compared

122 d K356Q Rubisco were similar in that spinach activase was much more effective than tobacco activase.

123 cv "Little Marvel") chloroplasts showed that activase was the major protein that denatured in respons

124 ase (Rubisco), which is regulated by Rubisco activase, was closely correlated with temperature-induce

125 e, and the activities of Rubisco and Rubisco activase were examined in species from contrasting envir

126 ygenase (Rubisco, EC 4.1.1.39), two chimeric activases were constructed, interchanging a Sensor 2-con

127 ested that there are two isoforms of Rubisco activase which may provide an explanation for the abilit

128 ter by recombinant Arabidopsis 43-kD Rubisco activase with the amino acid replacements Q111E and Q111