ライフサイエンスコーパス: active center

1 in a tiny, 5-nt-long RNA enzyme with a 3-nt active center.

2 ographically disordered loop adjacent to the active center.

3 nzymatic activities, albeit sharing a common active center.

4 ars to be a boundary between solvent and the active center.

5 snaking down from above the cleft toward the active center.

6 promotes NTP sequestration in the polymerase active center.

7 modulation of binding of Mg(2+) to the RNAP active center.

8 new contacts between the inner loop and the active center.

9 ricyanide to generate the Mo(V) state of the active center.

10 ve groups and coordination bonds to the RNAP active center.

11 ulls downstream DNA into itself and past its active center.

12 quired for the assembling and sealing of the active center.

13 NTP binding to an entry site adjacent to the active center.

14 , suggesting that it was bound to the enzyme active center.

15 ordination of catalytic Mg2+ ion in the RNAP active center.

16 tion of the Delta 24 bond occurs at the same active center.

17 he electronic and geometric structure of the active center.

18 TF on critical ionizations within the fVIIa active center.

19 ars to act as a smaller "gate" to the diiron active center.

20 a clamp, shown previously to swing over the active center.

21 xisomal targeting signal and a serine lipase active center.

22 onal flexibility of POR B in the surrounding active center.

23 diffusional entry of trifluoroketones to the active center.

24 ction of diffusional entry and exit from the active center.

25 9, that appear to grip DNA downstream of the active center.

26 tive Zn-coordination conformation around the active center.

27 nthesis by cleaving 23S rRNA in the ribosome active center.

28 ic group of Asp-135 side chain in the enzyme active center.

29 ith the nucleoside triphosphate (NTP) in the active center.

30 atalysis of individual reactions in the RNAP active center.

31 ize template-strand ssDNA to engage the RNAP active center.

32 ing of MBP to the thiamin diphosphate in the active centers.

33 ant in a large number of systems with mobile active centers.

34 er, they differ in the mode of action of the active centers.

35 nents, electron transfer pathways, and redox-active centers.

36 oms in the catalyst are captured to form the active centers.

37 fer processes and pathways between the redox-active centers.

38 this mechanism, roles are suggested for the active center acid-base groups D28, E477, H114, and H115

39 extrusion of the 3' end of the RNA from the active center after bond formation and before translocat

40 ed by the TrmD protein is mobilized into the active center after tRNA binding only when the AdoMet si

41 y to stabilize a short RNA-DNA hybrid in the active center, an increased frequency of abortive transc

42 ting enzyme (ACE) contain the same catalytic active center and are encoded by the same gene, whose di

43 maller side chains in the acyl pocket of the active center and find that the Phe295Leu substitution e

44 he nucleotide addition site, closing off the active center and forming an extensive network of intera

45 provides important insights into the protein active center and further evidence on the catalytic diff

46 (NOSs) from a "loose dimer", with an exposed active center and higher sensitivity to proteolysis, to

47 contains the 3' end of the transcript in the active center and is capable of binding the next cognate

48 binding, the LID domain moves away from the active center and makes the presumed ATP-binding site mo

49 ds to conformational changes induced by both active center and peripheral site inhibitors (gallamine

50 share a conserved Cys-X-X-Cys motif at their active center and play important roles in control of cel

51 ir closing of the trigger loop in the Pol II active center and polymerase translocation into the next

52 modulation of binding of Mg(2+) to the RNAP active center and presented three lines of biochemical e

53 P) by binding to a site adjacent to the RNAP active center and preventing synthesis of RNA products >

54 ite adjacent to but not overlapping the RNAP active center and stabilizes an RNAP-active-center confo

55 DNA hybrid in the RNA polymerase II (RNAPII) active center and, importantly, that the Tfg1-E346A subs

56 characterized yeast aptamer, is close to the active center and, thus, in an ideal position to regulat

57 characterized by a signature motif in their active centers and a two-base 3' overhang in their produ

58 ocesses with the simultaneous involvement of active centers and the bridging hydrogen, showing simila

59 he affinity of binding of Mg(2+) to the RNAP active center, and we reassess the three lines of bioche

60 he use of organic molecules as models of the active centers, and selective masking of functional grou

61 f TFIIB that is inserted into the polymerase active center; and a C-terminal domain, whose interactio

62 helix unwinding in advance of the polymerase active center; and extension of the diffraction limit of

63 Residue 262, being well removed from the active center, appears unaffected by inhibitor binding.

64 in hemoproteins and the way they bind to the active center are central to the protein's function.

65 Five alpha helices surrounding the active center are structurally homologous to the active

66 or a molecule in which the electrochemically active centers are in electronic communication.

67 the enzyme is probably a dimer in which the active centers are separated by about 40 A.

68 nsight to changes in hydrogen bonding at the active center as a result of substrate activation.

69 occurring at a localized, sequestered enzyme active center as previously shown, we demonstrate that t

70 This Mg(2+) is tightly bound to the active center aspartates, creating an inactive stable st

71 provide excellent evidence for nonidentical active centers (asymmetry) in solution in these multimer

72 base pairs of DNA-RNA hybrid extend from the active center at nearly right angles to the entering DNA

73 hemical characterization of RNAP explain the active center at the atomic level and enable new approac

74 s on acetylcholinesterase (AChE) comprise an active center, at the base of a deep and narrow gorge li

75 f apparently random change, during which its active center became modified.

76 Interactions of the NHA hydroxyl with active center beta-structure and the heme ring polarize

77 occurs when all recognition contacts of the active center-bound RNA segment are established and veri

78 We show that Sal interacts with the RNAP active-center 'bridge-helix cap' comprising the 'bridge-

79 tified, conserved hydrophobic patch near the active center but distinct from the ATP-binding cleft.

80 6-rings that act not only as a catalytically active center, but also as a dealumination suppressor.

81 firmed by the reconstitution of the synthase active center by using two heterologously expressed halv

82 s released from YPDC (assessing occupancy of active centers by these intermediates and rate-limiting

83 Asymmetry of active centers can also be induced upon addition of acet

84 that explains how FEN-1, which has a single active center, can have seemingly different activities i

85 enzyme where it is coordinated by the three active-center carboxylates.

86 is an essential goal of the investigation of active center chemistry and catalytic mechanism.

87 roles of noncovalent interactions in enzyme active center chemistry.

88 vidence that besides the catalytic site, the active center cleft also participates in the binding of

89 cts stabilize the DNA template strand in the active center cleft and/or position the RNAP clamp domai

90 es, which predicted that the residues of the active center cleft could, via electrostatic interaction

91 ng, further confirming the importance of the active center cleft for the PAP--ribosome and PAP--L3 in

92 gy to elucidate the putative role of the PAP active center cleft in the binding of PAP to the alpha-s

93 charged and polar side chains located at the active center cleft of PAP and certain catalytic site re

94 port provide unprecedented evidence that the active center cleft of PAP is important for its in vitro

95 PAP mutants with alanine substitution of the active center cleft residues (69)NN(70) (FLP-4) and (90)

96 ants of PAP with alanine substitution of the active center cleft residues N69 (FLP-20), F90 (FLP-21),

97 discovered that alanine substitutions of the active center cleft residues significantly impair the de

98 ) and (111)SR(112) that are distant from the active center cleft showed normal binding affinity to ri

99 egion of TFIIB, located above the polymerase active center cleft, but showing none of the B finger.

100 hat mediates opening and closing of the RNAP active center cleft--to prevent interaction of RNAP with

101 n complex, region 1.1 is located outside the active center cleft.

102 ions by interfering with opening of the RNAP active-center cleft during transcription initiation.

103 holoenzyme, region 1.1 is located within the active-center cleft, apparently serving as a "molecular

104 Rif SV are located within the RNA polymerase active-center cleft, overlapping the binding site for th

105 NA to be loaded into and unwound in the RNAP active-center cleft, that DNA loading and unwinding trig

106 sidue Glu636 has important functions both in active center communication and in protecting the active

107 t instantaneous configurations in the enzyme active center confirm the inferences made on the basis o

108 he RNAP active center and stabilizes an RNAP-active-center conformational state with a straight-bridg

109 equilibrium between the closed and the open active center conformations toward the closed form.

110 ight-bridge-helix and bent-bridge-helix RNAP-active-center conformations exist and that cycling betwe

111 ight-bridge-helix and bent-bridge-helix RNAP-active-center conformations is required for RNAP functio

112 d 36% identity with Grx1 and had a disulfide active center containing the Cys-Ser-Tyr-Cys motif.

113 g the functional competence of the E2pCD and active center coupling among E1p, LD, E2pCD, and E3 even

114 YPDC variants created by substitution at the active center (D28A, E51X, and E477Q) and on the substra

115 Within transposable elements, the only active center described, to date, facilitating both func

116 crystalline positions related to the typical active centers described for propane oxidation.

117 sidues 42-58, 183-199, and 278-298) near the active center displayed both EX1 (monomolecular) and EX2

118 ulating conformational changes in the RNAPII active center during initiation and early elongation.

119 s, how they affect molecular function of the active center during termination is incompletely underst

120 s-arginine is also recruited to complete the active center during this conformational change.

121 substitutions in the ADP-ribosyltransferase active center (E112K) and COOH-terminal KDEL (E112K/KDEV

122 ingle amino acid residue substitution in the active center eliminates the bias without noticeable eff

123 Thus, open conformation of the active center emerges as an intermediate essential for p

124 und that the two noncatalytic Cys around the active center exert an opposite yin-yang regulation on t

125 of HtrAs revealed a conserved pocket in the active center exhibiting pronounced proteolytic activity

126 Co, with precise and uniform distribution of active centers, exhibits not only high CO2 trapping capa

127 ium falciparum (PfTrxR) contains three redox active centers (FAD, Cys-88/Cys-93, and Cys-535/Cys-540)

128 the 3' terminus of RNA in the RNA polymerase active center, followed by the entry of a nucleoside tri

129 izontal lineP on the V(V)OPO4 surface is the active center for initiating the VPO chemistry through e

130 ligands located simultaneously in the hKAT I active center for the first time.

131 the large ribosomal subunit constitutes the active center for the protein folding activity of the ri

132 re due to atomic vacancies and can act as an active center for vacancy-driven gelation with a thiol-a

133 t of the rotor protein FliM but contains two active centers for CheY dephosphorylation.

134 This arrangement generates two active centers for fatty acid synthesis separated by int

135 Ge-S components in Li10GeP2S12 acting as the active centers for its cathode and anode performance, re

136 A clamp on the DNA nearer the active center, formed by Rpb1, Rpb2, and Rpb6, may be lo

137 g (i.e., abortive-product synthesis and RNAP-active-center forward translocation are fast, whereas ab

138 e center communication and in protecting the active center from undesirable "carboligase" side reacti

139 p at the peripheral site near the rim of the active center gorge (H287C); a second was in a helical r

140 econd was in a helical region outside of the active center gorge (T249C); a third was at the tip of a

141 uggest that the motions of residues near the active center gorge and across from the Cys(69)-Cys(96)

142 F295L/F297I/Y337A) were employed to enlarge active center gorge dimensions.

143 rganophosphate within the constraints of the active center gorge is a major factor in influencing oxi

144 in governing rates of ligand entry into the active center gorge of acetylcholinesterase and to chara

145 E; EC ), occluding the entry portal into the active center gorge of the enzyme and inhibiting its cat

146 t combined with the tacrine azide within the active center gorge to form multivalent inhibitors that

147 mutation to oximes within the space-impacted active center gorge with the aging resistance of the F33

148 The roles of four of the active center groups with potential acid-base properties

149 The active center (H-cluster) of [FeFe]-hydrogenases is embe

150 estigation, structural evidence defining the active center has been elusive.

151 ctra for single molecules adsorbed to single active centers have been reported in heterogeneous catal

152 e central dimerization domain containing the active center His residues (domain A) and the ATP-bindin

153 en bonding of ATP adenine to K in the closed active center), His(221) (covalent anchoring of dihydrox

154 The coordination of Co-Nx active centers hybridized with that of neighboring P ato

155 ectly to the bacterial RNA polymerase (RNAP) active-center 'i' and 'i+1' nucleotide binding sites, pr

156 xhibits strong coupling to the copper of the active center (I = 3/2) has been characterized by EPR.

157 ction may perturb the plasticity of the RNAP active center, implicating a role for omega and its flex

158 acidic residues stabilize the RNA polymerase active center in a catalytically inactive configuration

159 ial binding to an entry (E) site beneath the active center in an inverted orientation, followed by ro

160 s that ppGpp binds to the same site near the active center in both independent RNAP molecules in the

161 The weakly bound Mg(2+) is stabilized in the active center in different modes depending on the type o

162 alculations for possible redox states of the active center in Fe-only hydrogenases.

163 lack of understanding of the behavior of the active center in non-precious metal Fe-N/C catalysts und

164 ties are not fully connected into the diiron active center in the enzyme resting state, conformationa

165 s within the Saccharomyces cerevisiae RNAPII active center in the mechanism of transcription start si

166 Active centers in Cu/SSZ-13 selective catalytic reductio

167 is and degradation are performed by the same active center (in contrast to DNA polymerases in which t

168 Association of the active center inhibitor, tacrine, and the peripheral sit

169 The active center involves a symmetrical pair of Mg(2+) ions

170 The assembly of the active center involves large conformational changes in t

171 ts from the substitutions some 20 A from the active center is also seen in the first key step of the

172 eaction is product release, and the complete active center is assembled and sealed only upon the bind

173 n together, our studies suggest that the SMT active center is composed of a set of acidic amino acids

174 s sharply with previous suggestions that the active center is either the V-O bonds or else a chemisor

175 The active center is modeled by [(H(CH(3))S)(CO)(CN(-))Fe(p)

176 otes sequestration of NTPs in the polymerase active center just prior to the phosphodiester bond form

177 Active centers lie between K1 and L2 or K2 and L1: dihyd

178 crystallographic studies have shown that two active center loops (an inner loop formed by residues 40

179 and for a glycine in each of the two mobile active center loops of the E1 component, a 200-kDa homod

180 Substitutions in the active center loops produced variants with up to 900-fol

181 e analog known to affect the mobility of the active center loops, (b) an E2 component construct consi

182 A pore in the protein complex beneath the active center may allow entry of substrates for polymeri

183 that might play a role as an intermediate or active center may be useful.

184 cids actively contribute to the retention of active center Mg2+.

185 yacetone phosphate (DHAP) and of a number of active center mutants have resulted in a model of the ca

186 For patients enrolled by the 5 active centers (n = 1752), there was no improvement in l

187 substituted at positions D28 and E477 at the active center necessitate some modification of the mecha

188 bsence of Rad26, a lesion is "locked" in the active center of a Pol II elongation complex, which is s

189 transport of ions and small molecules to the active center of a protein or enzyme.

190 ions, Mg(2+) or Mn(2+), are required for the active center of FEN-1 nucleases.

191 Whereas inhibitors directed at the active center of gamma-secretase inhibit the cleavage of

192 A phosphorylates conserved Ser(239) near the active center of GltX and inhibits aminoacylation, a uni

193 The active center of LSD1 is characterized by a remarkable 1

194 Our findings offer detailed insights in the active center of MNK and serve as a structural basis to

195 d by an alanine-to-glutamine exchange in the active center of neisserial HtrA.

196 (SVTK, TEN, and KTG) that typically form the active center of other PBPs.

197 t segment of the fork domain involved in the active center of Pol II.

198 ivities are functionally integrated with the active center of Pol III during termination.

199 t this compound class does not bind into the active center of PqsD but in the ACoA channel, preventin

200 Site specific mutants in the pol active center of RB69 DNA polymerase have been produced

201 cleoside triphosphates (NTPs) diffuse to the active center of RNA polymerase II through a funnel-shap

202 factors located at a large distance from the active center of RNA polymerase II.

203 e has long been considered to constitute the active center of somatostatin, important both for recept

204 ector signals from nucleotide binding to the active center of TG2.

205 They further indicate that the active center of the adenylate kinase comprises ATP-bind

206 Dynamic loops at the active center of the E1 component of Escherichia coli py

207 The mutations clustered around the active center of the enzyme or resided at the surface of

208 largest subunit of RNA polymerase I near the active center of the enzyme that results in an elongatio

209 e GMP-binding domain, which moves toward the active center of the enzyme upon GMP binding, the LID do

210 o a disengagement of the propeptide from the active center of the enzyme, causing its activation.

211 of small molecules both into and out of the active center of the enzyme.

212 ut mouse line by targeting the exon encoding active center of the enzyme.

213 important for anchoring the substrate at the active center of the enzyme.

214 PP bind at one end of the channel, where the active center of the first half-reaction is located, and

215 Presenilins, which constitute the active center of the gamma-secretase complex, signal pep

216 Furthermore, this study indicates that the active center of the Hermes transposase differs from the

217 tial trace mineral selenium, which is at the active center of the iodothyronine deiodinase enzymes th

218 oop termed the "B finger," reaching into the active center of the polymerase where it may interact wi

219 ment at the 3' end of the nascent RNA in the active center of the yeast RNAP II, and TFIIS(AA) promot

220 Mutation E68Q or E255Q in the active centers of A3G/B resulted in loss of the inhibito

221 demonstrated tertiary lymphoneogenesis with active centers of cellular proliferation.

222 e fraction of radical intermediate occupying active centers of E1o-h are consistent with each other a

223 A comparative analysis of the active centers of hGSTA1-1 and hGSTA3-3 reveals that res

224 ems to account for the multiplication of the active centers of polymerization.

225 t atomic surface structures exhibit isolated active centers of single atomic and three atomic Pd ense

226 on of the three-dimensional structure at the active centers of the E. coli E1 subunit and transketola

227 ons of cysteine residues, their roles in the active centers of the enzymes responsible for deoxyribon

228 s of thiamin diphosphates (ThDPs) in the two active centers of the Escherichia coli pyruvate dehydrog

229 s readily and holds together the two dynamic active centers of the fatty acid synthase dimer, therefo

230 pyruvate decarboxylase, suggesting that the active centers of the two sets of enzymes exhibit differ

231 inity despite this residue constituting the "active center" of somatostatin peptides.

232 The nature of an active center on the surface of a working heterogeneous

233 ght into the pathways limiting the number of active centers on the surface of a heterogeneous catalys

234 ve holder as well as an accumulator of redox active centers on the surface of the electrode, and SWCN

235 research dollars available and even the most active centers only see a few patients per year.

236 resistance, such as substitutions in the NA active center or in the hemagglutinin.

237 are uncoupled and that ligand binding at the active center or the peripheral site does not influence

238 tive center loop removing constraints on the active center P1 residue.

239 n NADPH-dependent homodimer with three redox-active centers per subunit: a FAD, an N-terminal domain

240 igger loop (TL) in the RNA polymerase (RNAP) active center plays key roles in the reactions of nucleo

241 e (HPPK) is required for the assembly of the active center, plays an important role in the stabilizat

242 site several nucleotides downstream from the active center position when sigma70 first engages the -1

243 ed sequestration of the NTP substrate in the active center prior to catalysis.

244 The adenylate kinase active center probe P(1),P(5)-di(adenosine-5') pentaphos

245 while moderate Lewis acidity/basicity at the active centers promotes catalysis by releasing the reduc

246 We hypothesize that these active center-proximal contacts stabilize the DNA templa

247 omains of this subunit, probably through the active center region of the enzyme.

248 eatly enhances the lifetime of an NTP in the active center region, and it prevents "backtracking" and

249 helices) couple to conformational changes in active center residues (I helix) implicated in proton pu

250 f mKAT III and its cofactor binding site and active center residues.

251 2)O to the empty site in the enzyme's diiron active center results in an oxidized inactive form (H(2)

252 ereas abortive-product dissociation and RNAP-active-center reverse translocation are slow).

253 Steric constraints in the active center rule against superoxo-iron accepting a hyd

254 oxon results in covalent modification of the active center serine and a corresponding increase in mol

255 Further, the tryptic peptide containing the active center serine of AChE, isolated from mouse brain

256 e tryptic peptide of mAChE that contains the active center serine residue resolves to a molecular mas

257 r, phosphorylation and carbamoylation of the active center serine shows distinctive changes in acrylo

258 dance of AChE peptides containing a modified active center serine strongly correlates with the fracti

259 p conformations accompany conjugation at the active center serine.

260 olinesterase by reacting covalently with the active center serine.

261 angements of bridge helix, trigger loop, and active-center side chains that isomerize the triphosphat

262 al change is that loop 3 moves away from the active center significantly with some residues moving by

263 Comparison of the active-center structure with that of the 6-phospho-alpha

264 wo structurally different forms of the Mo(V) active center termed the low-pH (lpH) and high-pH (hpH)

265 to the paucity of acid-base residues at the active center that can participate in proton transfer st

266 g site on RNAP includes residues of the RNAP active center that cannot be substituted without loss of

267 ncognate residue, which is hydrolyzed by the active center that carries two Mg(2+) ions.

268 ess to the cosubstrate binding pocket and an active center that contains a 3-Asp putative metal bindi

269 Their device signal is dominated by an active center that inverts affinity upon guest incorpora

270 d in which ppGpp prevents the closure of the active center that is induced by the binding of NTP, whi

271 n RNAp-promoter interactions around the RNAp active center that likely occur due to repositioning of

272 rged nucleic acid binding site distal to the active center that plays a key role in substrate DNA bin

273 Moreover, the Lecsl had one active center that served to bind two kinds of substrate

274 ate DNA strand one nucleotide ahead from the active center (the i+2 position).

275 At the heart of this machine is the active center, the engine, which is composed of distinct

276 beta domain can transfer information to the active center thiamin diphosphate (ThDP) located at the

277 ocated on the beta domain some 20 A from the active center thiamin diphosphate cofactor, which is at

278 to H92, to E91, to W412, and finally to the active center thiamin diphosphate.

279 target peptide might gain access to the PDHK active center through the open but not through the close

280 ng over a distance of 90 A from the G domain active center to domain IV.

281 DNA transposases use a single active center to sequentially cleave the transposable el

282 nucleotides of mRNA extending from the RNAP active center to the ribosome decoding center.

283 hat putative alternative mechanisms for RNAP active-center translocation in initial transcription, in

284 Such a rearrangement that we call active center tuning (ACT) occurs when all recognition c

285 in-induced interaction was identified on E1p active centers upon assembly with E2p and C-terminally t

286 recently reported that addition to the E477Q active-center variant of yeast pyruvate decarboxylase of

287 Studies with YPDC and PDHc-E1, and their active center variants, in conjunction with chemical mod

288 enzyme that allows substrates to access the active center via a 22-A deep channel.

289 6A substitutions, ThDP binding in the second active center was affected.

290 d binding of the second ThDP, once the first active center was filled, suggesting a pathway for commu

291 The two inner beta-rings harbor active centers, whereas the two outer alpha-rings play a

292 de triphosphate-binding site adjacent to the active center, which may serve as a gateway for polymeri

293 are in the vicinity of the binuclear Mn(2+) active center, which provides detailed insight into how

294 eviously localized near Rpc2p in the pol III active center while a minority represent a distinct clas

295 The active center with a semi-closed conformation binds a ph

296 to shield the substrate binding sites in its active center with negatively charged polyelectrolyte.

297 e formation by TFIIH, which fills the Pol II active center with single-stranded DNA, and subsequent s

298 ry questions remain obscure: 1) What are the active centers with respect to various defect species an

299 ty and coordination, but maintain accessible active centers with uniform structure and unrestricted a

300 products formed by the E477Q, D28A, and D28N active center YPDC variants was undertaken.