ライフサイエンスコーパス: active enzyme

1 n and subsequent generation of catalytically active enzyme.

2 o mammalian aconitase (mACO2) to generate an active enzyme.

3 e oxidase-molybdenum domain yielding a fully active enzyme.

4 rap the fluorochromes in the vicinity of the active enzyme.

5 +) and calmodulin-independent constitutively active enzyme.

6 CYP2F1 is an active enzyme.

7 mitting heme binding and dimerization to the active enzyme.

8 eletions of 1-24 amino acids produce a fully active enzyme.

9 ing individual steps in its conversion to an active enzyme.

10 % of the rate of formation to regenerate the active enzyme.

11 expressed in Escherichia coli and yielded an active enzyme.

12 ed nine orthologues of known subunits but no active enzyme.

13 transforms the zymogen into a catalytically active enzyme.

14 TPase proteins associate preferentially with active enzyme.

15 so has sensitivity to detect up to 2.5 ng of active enzyme.

16 ptide complementation to produce a partially active enzyme.

17 Zn(2+), Co(2+), Ni(2+), and Cd(2+)-generated active enzyme.

18 e that undergoes autoprocessing to become an active enzyme.

19 ays be efficiently reduced to regenerate the active enzyme.

20 MP proenzyme noticeably exceeded that of the active enzyme.

21 d its cleavage at D175 no longer leads to an active enzyme.

22 active form, whilst ScKAT is the reduced and active enzyme.

23 gle population of normally assembled, highly active enzyme.

24 of two modes of functional assembly for the active enzyme.

25 finities inferred from kinetic studies using active enzyme.

26 oes not require its prodomain for folding to active enzyme.

27 e xdhAB genes in E. coli JM109(DE3) produced active enzyme.

28 recombinant expression as a soluble, highly active enzyme.

29 nt temperature of the reduced, catalytically active enzyme.

30 r to turn only a fraction of a degree in the active enzyme.

31 locluster composition of HydG that comprises active enzyme.

32 (TERT), minimized hTR assembled biologically active enzyme.

33 id binding domain, produced a constitutively active enzyme.

34 inucleotide (FAD), which was not the part of active enzyme.

35 s a new limiting factor in the production of active enzyme.

36 sts characterizing functionally carbohydrate-active enzymes.

37 gulare genome revealed over 280 carbohydrate-active enzymes.

38 wever, once stabilized, they are efficiently active enzymes.

39 ported before for this class of carbohydrate-active enzymes.

40 mosomal duplication and encode catalytically active enzymes.

41 furthers progress toward reliably producing active enzymes.

42 ial for the characterization of carbohydrate-active enzymes.

43 expansion of its repertoire of carbohydrate-active enzymes.

44 oughout numerous C. perfringens carbohydrate-active enzymes.

45 , indicating the expression of catalytically active enzymes.

46 mational fluctuations of single residues for active enzymes.

47 basic catalytic characteristics of the most active enzymes.

48 resource for discovering novel carbohydrate active enzymes.

49 gens, which are proteolytically processed to active enzymes.

50 resource for discovering novel carbohydrate active enzymes.

51 exon skipping and inclusion isoforms encode active enzymes.

52 n of an extensive repertoire of carbohydrate-active enzymes.

53 sequestration, and a number of carbohydrate-active enzymes.

54 thrin with either inorganic nanoparticles or active enzymes.

55 n observed for the protein scaffold of redox active enzymes.

56 ytosol (polyethylene glycol precipitation of active enzyme), (3) expression of a recombinant p62 acce

57 All active enzymes accepted scoulerine and tetrahydrocolumba

58 ed by direct chromatographic isolation of an active enzyme-acetyl species.

59 ) that is proteolytically processed into the active enzyme after exposure to low pH in vitro or targe

60 Article describes a strategy for quantifying active enzyme analytes in a paper-based device by measur

61 a 50 kDa precursor and processed to a 30 kDa active enzyme and 18 kDa propeptide, the effects of thes

62 that translocates to the cell surface as an active enzyme and co-localizes with cell surface annexin

63 se lead to significantly decreased levels of active enzyme and could be causal in the development of

64 methods for the generation of unique, highly active enzymes and also suggest a possible means of enzy

65 nd more focused annotations for carbohydrate-active enzymes and antibiotic resistance genes.

66 ome sequence reveals a suite of carbohydrate-active enzymes and demonstrates a level of diversity at

67 ctions, which are widespread in carbohydrate-active enzymes and have long been associated with proces

68 i OMVs are conduits of signalling molecules, active enzymes and other proteins to its environment.

69 Transcript abundance of several carbohydrate-active enzymes and phenylalanine ammonia-lyases was also

70 e genes, such as those encoding carbohydrate-active enzymes and proteases, can be predicted from bact

71 quence reveals a diverse set of carbohydrate-active enzymes and provides further insight into lignoce

72 nd thiamine, and genes encoding carbohydrate active enzymes and secondary metabolism enzymes.

73 i possessed substantially fewer carbohydrate-active enzymes and secreted proteins than closely relate

74 succinogenes are enriched with carbohydrate-active enzymes and that intact OMVs were able to depolym

75 ed to estimate the relative concentration of active enzymes and variation in response to environmenta

76 conversion of the latent proenzyme into the active enzyme, and also via inhibition by tissue inhibit

77 beta building block can be assembled into an active enzyme, and provides further insight into the mol

78 e of one kinase molecule, which serves as an active enzyme, and specific docking sites on the C-lobe

79 n of substrate specificities of carbohydrate-active enzymes, and further explored using defined oligo

80 with an H69E mutation (PR(H69E)) folds into active enzymes, and it does so with an apparent Kd (diss

81 MYBs and identify transporters, carbohydrate-active enzymes, and small peptides as candidate molecula

82 , N106Q and K170R, resulted in catalytically active enzymes, and these active mutant enzymes gave pH-

83 beyond glycoside hydrolase and carbohydrate active enzymes, and to include a newly identified functi

84 imary and secondary metabolism, carbohydrate-active enzymes, and transporters, probably reflecting th

85 Our results provide insights into active enzyme architecture, explain biological variation

86 As sufficiently active enzymes are currently unknown, we synthesized twe

87 Many T. reesei genes encoding carbohydrate-active enzymes are distributed nonrandomly in clusters t

88 1 mRNA led to a full-length, Pyl-containing, active enzyme as determined by immunoblotting, mass spec

89 color but has the same molecular mass as the active enzyme as determined by mass spectrometry.

90 as a model, we show that FAS foci represent active enzyme assemblies.

91 alytic processing in maintaining the pool of active enzyme at the cell surface.

92 MP endocytosis resulting in higher levels of active enzyme at the cell surface.

93 diverse probe and protein families to detect active enzymes at scale and resolution out of reach with

94 The ATCC 14579 genome encodes an active enzyme Bce14579I (GCWGC).

95 This regulation requires the presence of active enzyme, because mutated forms of heparanase lacki

96 e addition of Mn2+, Co2+, and Cu2+ generated active enzymes, but the addition of Zn2+, Fe2+, and Cd2+

97 Analysis of the purified, active enzyme by inductively coupled plasma-emission spe

98 t has been previously reported, but released active enzyme can also modulate cytokine expression, whi

99 PqqE isolated anaerobically yields an active enzyme capable of cleaving SAM to methionine and

100 In the Carbohydrate-Active Enzyme (CAZy) database, glycoside hydrolase famil

101 e superfamily (according to the Carbohydrate-Active Enzymes (CAZy) data base).

102 Based on the Carbohydrate-Active enZymes (CAZy) database, the genome of the refere

103 Carbohydrate-active enzymes (CAZymes) are very important to the biote

104 We describe here analysis of Carbohydrate Active Enzymes (CAZymes) from 3.5 gigabase sequences of

105 o identify reaction products of carbohydrate active enzymes (CAZymes) of the filamentous fungus Asper

106 as a promising source of novel carbohydrate active enzymes (CAZymes) that modify plant cell wall pol

107 ins encode a narrow spectrum of carbohydrate active enzymes (CAZymes) that reflect extreme specializa

108 ll secreted proteins (SSPs) and carbohydrate-active enzymes (CAZymes) were the two major classes of e

109 arides possess large numbers of carbohydrate active enzymes (CAZymes), many of which have been catego

110 encoding a large contingent of Carbohydrate-Active enZymes (CAZymes), many of whose specific functio

111 rain, which were overwhelmingly carbohydrate active enzymes (CAZymes), with 95 large fungal scaffoldi

112 bors an impressive reservoir of carbohydrate active enzymes (CAZymes).

113 This was also the case for carbohydrate-active enzymes (CAZymes).

114 merous candidate peptidases and carbohydrate-active enzymes ('CAZymes') (9) targeted for secretion.

115 and NF-kappaB activation by modulating redox-active enzymes, cell migration, phagocytosis, and bioene

116 o build phylogenies for CAZyme (carbohydrate active enzyme) classes and Pfam clans, which attested it

117 Redox-active enzyme cofactors derived from ribonucleotides hav

118 Analysis of the carbohydrate active enzyme complement suggests an ability to utilize

119 ed to the membrane and recruits WbdA into an active enzyme complex by protein-protein interactions.

120 d POMT2, two of the causative genes, form an active enzyme complex in the posttranslational biosynthe

121 We found that NSI self assembles into highly active enzyme complexes and that high concentrations of

122 it associates with cyclin D and p57(Kip2) in active enzyme complexes.

123 ceptor complexes, where they produce a fully active enzyme composed of two catalytic domains.

124 comprehensive understanding of catalytically active enzyme composition, we performed affinity purific

125 logic concentrations decreases the amount of active enzyme concentrations.

126 the mechanism by which the final key-lock or active enzyme conformation is achieved upon formation of

127 tional dual Src/Abl inhibitors targeting the active enzyme conformation, these inhibitors bind to the

128 troscopy, have been aimed at elucidating the active enzyme conformation.

129 ces of the nanocage enhance the stability of active enzyme conformations through the action of a stro

130 erms of its activation by Na+ binding to the active enzyme conformer at an allosteric site, inhibitio

131 and inhibition by lysine binding to the less active enzyme conformer.

132 , features associated with the catalytically active, enzyme-coordinated copper center were evident be

133 The obtained active enzyme CrHydA1(adt) shows the same redox states i

134 c distances, and queries to the carbohydrate-active enzymes database (www.cazy.org), our predictor ac

135 filing of some members from the Carbohydrate-Active Enzymes database glycosyl transferase families GT

136 fore, are not yet assigned to a Carbohydrate-Active Enzymes database GT family.

137 The Carbohydrate Active Enzymes Database indicates that Lam81A belongs to

138 nsferase family, GT97, in CAZy (Carbohydrate-Active enZYmes Database).

139 osyltransferase families in the Carbohydrate-Active Enzymes database.

140 The ThYme (Thioester-active enzYme) database has been constructed to bring to

141 of E. granulatus is reduced in carbohydrate-active enzymes, despite a large expansion in genome size

142 , providing support for a model proposing an active enzyme dimer.

143 that a PS1/Pen2/Aph1a trimeric complex is an active enzyme, displaying biochemical properties similar

144 of the O(2)-tolerance mechanism regenerates active enzyme, effectively completing a competitive four

145 e backside of the membrane has access to the active enzyme embedded in the electrode.

146 Like all redox-active enzymes, Erv1 requires one or more electron accep

147 However, active enzyme exhibited a molecular mass of approximatel

148 The active enzyme exists primarily as a dimer, and the histi

149 ponent, i.e., highly purified, catalytically active enzyme expressed in a baculovirus system.

150 to detect and analyze the activity of redox active enzymes expressed on the surface of E. coli.

151 tive sites for both zymogen factor X and the active enzyme, factor Xa.

152 origins and activities of other carbohydrate active enzyme families and the biological significance o

153 pid P450-substrate exchange, except with the active enzyme Fe-O complex.

154 To obtain a large quantity of active enzyme for substrate screening, we overexpressed

155 ased yields provide sufficient quantities of active enzyme for use in nuclear magnetic resonance (NMR

156 drate esterases, as well as accessory, redox-active enzymes for lignin depolymerization.

157 ructs were explored to identify an optimally active enzyme form for inhibition and structural studies

158 ite which is present in the glucose-induced, active enzyme form of GK and absent in the inactive apoe

159 ate stabilize an active tetramer over a less active enzyme form of mass comparable with that of a dim

160 which ATP hydrolysis is used to generate an active enzyme form that hydrolyzes peptide.

161 on of proprotein convertase 2 (proPC2) to an active enzyme form; this action is accomplished via its

162 ion of up to 90 mg of homogeneous and highly active enzyme from 10 g of cell material.

163 central nervous system gene therapy, because active enzyme from genetically corrected cells can be se

164 otal amount of BChE (including inhibited and active enzyme) from 70 muLpost-exposure human plasma sam

165 e time courses of the amounts of product and active enzyme, from which the dynamic ranges can be obta

166 When converted to the active enzyme, FXIa, all the monomeric mutants activated

167 s, including the acquisition of a putatively active enzyme, give the mitoribosome a distinct architec

168 ad to the inactivation of the constitutively active enzyme, glycogen synthase kinase-3 (GSK3), which

169 oires of two different types of carbohydrate-active enzymes, glycoside hydrolases and glycosyltransfe

170 ases (GALTs) are members of the carbohydrate-active enzyme glycosyltransferase (GT) 31 family (CAZy G

171 Carbohydrate-active enzyme glycosyltransferase family 8 (GT8) include

172 Indeed, the lyase-active enzyme has 3 orders of magnitude higher affinity

173 erest, but other types of proteins including active enzymes have also been expressed.

174 Active enzymes have been successfully immobilized for in

175 Carbohydrate-active enzymes have multiple biological roles and indust

176 anisms, suggesting that these day- and night-active enzymes have undergone thermal adaptation.

177 We uncover a complex set of carbohydrate-active enzymes, identify the genes required for the meth

178 examine heme binding by apo-nNOS to form the active enzyme in a cell-free system.

179 of chelating resin, generating catalytically active enzyme in a process that appeared to involve dire

180 ctly to the Psi-synthase to form the minimal active enzyme in archaea.

181 P4H1 can be met by forced expression of the active enzyme in either pre-stalk (anterior) or pre-spor

182 human COT kinase that yields pure and highly active enzyme in sufficient yields for biochemical and s

183 the proposal that JAK2 is an inefficient but active enzyme in the absence of activation loop phosphor

184 While we did detect active enzyme in the brain following peripheral administ

185 propeptide lead to the secretion of a fully active enzyme in the cytosol of host cells independent o

186 ferase impair retention of the catalytically active enzyme in the Golgi complex.

187 s nuclear-encoded protein is converted to an active enzyme in the mitochondrial matrix.

188 haea can be transcribed and translated to an active enzyme in the native organisms.

189 d then allows it to be released as a soluble active enzyme in the periplasm.

190 The percentage of active enzyme in the preparations was also comparable, a

191 produced in Escherichia coli as soluble and active enzyme in the presence of its natural inhibitor a

192 scort the transition of MASP from zymogen to active enzyme in the PRM/MASP complex; rather, clusterin

193 ssed Nox4 represents the only constitutively active enzyme in this family, analysis of structural det

194 Using PyABPs, we were able to profile active enzymes in rat liver microsomes and identify pyre

195 Plasma lipoproteins carry a number of highly active enzymes in the circulation.

196 i.e., mutations preventing the generation of active enzyme) in MLII and with mild mutations (i.e., mu

197 (i.e., mutations allowing the generation of active enzyme) in MLIIIA.

198 ber of large and highly modular carbohydrate-active enzymes, including the mu-toxin and sialidases, w

199 slation with correctly acylated aa-tRNA) and active enzyme indistinguishable from the wild-type prote

200 ls and determined that it is a catalytically active enzyme, inhibited by beta-amino-proprionitrile (B

201 The active enzyme is a 1:1 complex of two homodimeric subuni

202 zi telomerase suggest that the catalytically active enzyme is a large ribonucleoprotein complex and t

203 es that the monomer is inactive and that the active enzyme is at least a dimer.

204 teases mature from an inactive zymogen to an active enzyme is expected to inform new strategies for b

205 control of SBT3 activity, to ensure that the active enzyme is not released before it reaches the acid

206 p after chemistry but before regeneration of active enzyme is rate-limiting for synthesis of Gln-tRNA

207 ble universal fingerprint shared by all cold-active enzymes is a reduction of the activation enthalpy

208 osynthetic gene clusters and antibacterially active enzymes is discussed here.

209 ary model wherein the appearance of the most active enzyme isoform, glutaminase C (GAC), which is exp

210 ither cDNA transfection or microinjection of active enzyme, leads to their transformation.

211 means to study the presence and location of active enzyme levels in different cell types, such as he

212 The active enzyme loading levels were tracked using an activ

213 lular secretion and proteolysis, derives the active enzyme (Lox) and the propeptide (Lox-PP).

214 rsor Pro-LOX that is processed to the 32-kDa active enzyme (LOX) and to an 18-kDa propeptide (LOX-PP)

215 Concentrations of active enzymes (matrix metalloproteinase [MMP]-8, elasta

216 yme F430, which is in the Ni(I) state in the active enzyme (MCRred1).

217 eductase (PFOR), suggesting that other redox active enzymes mediate this reaction.

218 the model suggests that the vast majority of active enzyme molecules in a population should be in the

219 romoter control resulted in self-assembling, active enzyme multimers.

220 , undergo alternative splicing, resulting in active enzymes named isoforms 1 (i1s) and novel truncate

221 tility and effectiveness of the carbohydrate-active enzymes of T. reesei, its genome encodes fewer ce

222 nic biotransformation, suggesting that other active enzymes of the AD process are also involved in OM

223 acteria produce a wide range of carbohydrate-active enzymes, of which glycoside hydrolases are the ma

224 ing uniform and reproducible coatings of the active enzyme on the miniature transducer element.

225 ut microbiome--which encode the carbohydrate active enzymes, or CAZymes, that are absent from the hum

226 In addition to the expected carbohydrate active enzymes, our new method reveals a large number of

227 In the active enzyme, phosphate release from the beta(E)-subuni

228 The membrane-active enzyme phospholipase D (PLD) catalyzes the hydrol

229 ogen bound to ErpP could be converted to the active enzyme, plasmin.

230 namics of the denitrifier population and the active enzyme pool, which controlled the rate function.

231 ent including the repertoire of carbohydrate active enzymes present within and between twins (e.g., p

232 ese data provide evidence for a biologically active enzyme product 7alpha-hydroxyPREG and suggests th

233 LOX has tumor-suppressor activity, while the active enzyme promotes metastasis.

234 This strategy, combined with active enzyme, provides a new chemoenzymatic route to th

235 , but decrease the fraction of catalytically active enzyme recovered in the limit of long time.

236 bstrate accessibility and complementation by active enzymes retargeted to a companion organelle.

237 liary cell components, but rapid assembly of active enzyme seemingly is assisted in the cell.

238 An active enzyme structure is thus assembled at the common

239 buffer but also a cofactor for several redox active enzymes such as glutathione S-transferase and glu

240 Carbohydrate-active enzymes such as glycoside hydrolases (GHs) and gl

241 ymes are also found among these carbohydrate-active enzymes, such as chitinases, chitobiases, and lyt

242 Active enzymes, such as proteases, often serve as valuab

243 ul for predicting mechanisms for other redox-active enzymes, such as the one for photosystem II, but

244 Analyses of carbohydrate active enzymes suggest that this fungus is a biotroph wi

245 the three beta subunit isoforms and form an active enzyme, suggesting the absence of selective alpha

246 ended polypeptides are putative carbohydrate-active enzymes, suggesting a potential role in carbohydr

247 The 2.66-A crystal structure of the most active enzyme suggests that the engineered active site c

248 ional bioinorganic hybrid nanostructures via active enzyme-templated synthesis of inorganic nanomater

249 ising allosteric mechanism that destabilizes active enzyme tetramer.

250 Its genome encoded more carbohydrate active enzymes than any previously sequenced member of t

251 Mutation of Asp101 results in an active enzyme that incorporates metal in vivo but shows

252 d to virion incorporation of a catalytically active enzyme that is directly involved with Vpr in modu

253 uinolone-resistant A. thaliana gyrase yields active enzyme that is resistant to ciprofloxacin.

254 study, the PL2 domain was found to encode an active enzyme that mediates efficient processing of nsp2

255 Several cell wall enzymes are carbohydrate active enzymes that contain a putative Carbohydrate Bind

256 deleted G9a isoforms were also catalytically active enzymes that methylated recombinant H3 or synthet

257 es constitute a ubiquitous class of membrane-active enzymes that play a key role in cellular signalin

258 ino acid residues 400-415) are catalytically active enzymes that retain the 5' to 3' scanning ability

259 common protein motifs found in carbohydrate-active enzymes that synthesize or depolymerize polysacch

260 This change requires a fully active enzyme, the correct cofactor and the 2-aminopurin

261 interactions are present in all carbohydrate-active enzymes, these results have implications for unde

262 n inert polypeptide is converted to a mature active enzyme through post-translational modifications.

263 ramatically stimulate the function of weakly active enzymes through complex formation.

264 helix, allowing the catalytic domain of the active enzyme to cleave the collagen alpha chains sequen

265 that can hetero-oligomerize with and target active enzyme to membranes.

266 oy invasive growth and powerful carbohydrate active enzymes to reduce multicellular plant tissues to

267 iochemical descriptions of the 'active-to-de-active' enzyme transition that occurs during hypoxia.

268 duck hepatitis B virus polymerase employing active enzyme translated in vitro and derived from intra

269 treated cells, consistent with regulation of active enzyme turnover by synthetic MMP inhibitors.

270 general base), and the mechanism of the most active enzyme variant, were characterized.

271 identification of clones encoding unusually active enzyme variants.

272 n by GAGs and suggest that generation of the active enzyme via autoactivation can be accomplished not

273 te for the conversion of the zymogen form to active enzyme was also identified between R275 and I276,

274 The active enzyme was isolated and identified as Arg-gingipa

275 MT1-MMP mRNA and active enzyme was quantified in placentas from FGR and a

276 To investigate rotational dynamics of the active enzyme, we labeled SERCA with erythrosin 5'-iodoa

277 Following secretion, the latent zymogen and active enzyme were each purified from media by fast prot

278 on during leukocyte phagocytosis to form the active enzyme were well described, leading to the identi

279 glycoside hydrolases and other carbohydrate active enzymes were known to reside within the celluloso

280 and nearby interface residues, catalytically active enzymes were recoverable for approximately 70% of

281 generated against the soluble, catalytically active enzyme, whereas Abs reactive with cell surface-as

282 ociation of the fragments in the assay forms active enzyme, which acts on substrate to generate a col

283 agments in the assay results in formation of active enzyme, which acts on substrate to generate a det

284 or containing glpTQ from B. hermsii produced active enzyme, which demonstrated the association of glp

285 peptide bond is the key to formation of the active enzyme, which involves increased dynamics of the

286 ition, we decipher the process leading to an active enzyme, which involves the activation of the apo-

287 st that glycosylated linkers in carbohydrate-active enzymes, which are intrinsically disordered prote

288 E. weberi genome is depleted in carbohydrate active enzymes, which is consistent with reliance on a h

289 mes was established, with GAC being the most active enzyme while forming the longest structures.

290 The need to immobilize active enzyme, while ensuring high rates of substrate tu

291 of the [FeFe] hydrogenases by assembling the active enzyme with a version of the active site synthesi

292 Mutation of Thr162 to Ala results in an active enzyme with no cooperativity.

293 idyl peptidase I, storage, and release as an active enzyme with the three active proteases.

294 hanism involves selective association of the active enzyme with transcriptionally active nucleoids an

295 The deletion mutants produce correct-sized, active enzymes with a good correlation between enzyme am

296 genes encode almost identical, catalytically active enzymes with distinctive N-terminal peptide seque

297 ion mutant) is recovered as a constitutively active enzyme, with high basal autocatalytic activity an

298 trate efforts to design and engineer stable, active enzymes without laborious high-throughput activit

299 olar to nanomolar concentrations of membrane-active enzymes without requiring labeled substrates or p

300 converted nitrosylated GAPDH to the reduced, active enzyme, without forming any glutathionylated GAPD