ライフサイエンスコーパス: acyl-CoA synthetase

1 the enzyme is a broad substrate specificity acyl-CoA synthetase.

2 was to use triacsin C to inhibit long-chain acyl-CoA synthetase.

3 d by an inhibitor (triacsin C) of long chain acyl-CoA synthetase.

4 imilarity to the Escherichia coli long chain acyl-CoA synthetase.

5 it encodes LACS2, a member of the long-chain acyl-CoA synthetases.

6 wo highly conserved motifs characteristic of acyl-CoA synthetases.

7 ptide synthetases, luciferase, and aryl- and acyl-CoA synthetases.

8 the defective gene had sequence homology to acyl-CoA synthetases.

9 Firefly luciferase is homologous to fatty acyl-CoA synthetases.

10 and murine homologs comprise a new family of acyl-CoA synthetases.

11 ultigene family of very long chain (C20-C26) acyl-CoA synthetases.

12 for RpPat, all of which are also AMP-forming acyl-CoA synthetases.

13 SrtN) regulate the activities of AMP-forming acyl-CoA synthetases.

14 ing Peri A and HSL but stably overexpressing acyl-CoA synthetase 1 (ACS1) and fatty acid transport pr

15 hearts with a temporally induced knockout of acyl-CoA synthetase 1 (Acsl1(T-/-)) are virtually unable

16 The enzyme acyl-CoA synthetase 1 (ACSL1) is induced by peroxisome p

17 Long-chain acyl-CoA synthetase 1 (ACSL1) plays a key role in fatty

18 ates with increased expression of long-chain acyl-CoA synthetase 1 (ACSL1), an enzyme that catalyzes

19 genous perilipins and HSL but overexpressing acyl-CoA synthetase 1 and fatty acid transporter 1.

20 ipotoxicity overexpressing ACSL1 (long-chain acyl-CoA synthetase 1) in cardiomyocytes, we show that m

21 ACSL1 (acyl-CoA synthetase 1), the major acyl-CoA synthetase of

22 We also increased the levels of acyl-CoA synthetase-1 (ACS1) and ACS2 proteins to determ

23 that requires activation by very long-chain acyl-CoA synthetase-1 (ACSVL1) to modulate both targets,

24 sters GLUT4 in fat cells contains long chain acyl-CoA synthetase-1 and its product fatty acyl-CoA, sh

25 ectrometry of a p75 protein band, long chain acyl-CoA synthetase-1, specifically present in immunoads

26 y increased the beta-cell mRNA expression of acyl CoA synthetase-2 and peroxisomal acyl CoA oxidase i

27 Here, we show that long chain acyl-CoA synthetase 3 (ACSL3) plays a crucial role in se

28 transfected with control siRNA, knockdown of acyl-CoA synthetase 3 (ACSL3) significantly decreased re

29 A comparative analysis demonstrates that the acyl-CoA synthetase 3 is recruited early to the assembly

30 rial HMG-CoA synthase and increases in fatty acyl-CoA synthetase (3-8-fold) and carnitine palmitoyl-C

31 Here we show that long-chain acyl-CoA synthetase 4a (Acsl4a), an LC-PUFA activating e

32 Long-chain acyl-CoA synthetase 6 (ACSL6) mRNA is present in human a

33 KEY POINTS: Long-chain acyl-CoA synthetase 6 (ACSL6) mRNA is present in human a

34 th basal and Wy-14,643-induced expression of acyl-CoA synthetase, a well characterized PPARalpha targ

35 s were generated in two domains conserved in acyl-CoA synthetases: a 6- amino acid substitution into

36 The NDP-forming acyl-CoA synthetases (ACDs) catalyze the conversion of v

37 We show that the acyl-CoA synthetase ACS-7, which localizes to lysosome-r

38 CoA toxicity in mycobacteria by inactivating acyl CoA synthetase (ACS).

39 uggested that FATP1 exhibits very long chain acyl-CoA synthetase (ACS) activity and that such activit

40 Long chain acyl-CoA synthetase (ACS) and diacylglycerol acyltransfe

41 Acyl-CoA synthetase (ACS) enhances the uptake of FAs by

42 Inhibition studies have suggested that acyl-CoA synthetase (ACS, EC ) isoforms might regulate t

43 omes and FA synthesis in the cytosol, namely acyl-CoA synthetase (acs-1 and acs-2), acyl-CoA oxidase

44 stence of inhibitor-sensitive and -resistant acyl-CoA synthetases (ACS, EC ) that are linked to speci

45 A cDNA encoding a novel isoform of acyl-CoA synthetase (ACS6) was isolated from embryos of

46 uction in 20:4n-6-CoA mass and in microsomal acyl-CoA synthetase (Acsl) activity toward 20:4n-6.

47 contained not only CPT1a but also long chain acyl-CoA synthetase (ACSL) and the voltage-dependent ani

48 Long chain acyl-CoA synthetase (ACSL) catalyzes the initial step in

49 ACSL3 is a member of the long chain acyl-CoA synthetase (ACSL) family that plays key roles i

50 s directly inhibiting recombinant long-chain acyl-CoA synthetase (ACSL)-4 activity.

51 ng myelin, requires activation by long-chain acyl-CoA synthetase (ACSL).

52 tal muscle-specific deficiency of long-chain acyl-CoA synthetase (ACSL)1.

53 Long-chain acyl-CoA synthetases (ACSL 1 to 6) are key enzymes regul

54 ABSTRACT: Long-chain acyl-CoA synthetases (ACSL 1 to 6) are key enzymes regul

55 Long chain acyl-CoA synthetases (ACSL) activate fatty acids (FA) an

56 Long chain acyl-CoA synthetases (ACSL) and fatty acid transport pro

57 Long-chain acyl-CoA synthetases (ACSLs) are key host-cell enzymes t

58 In mammals, a family of five acyl-CoA synthetases (ACSLs), each the product of a sepa

59 is the conversion to acyl-CoA by long chain acyl-CoA synthetases (Acsls).

60 he first crystal structure of a medium-chain acyl-CoA synthetase ACSM2A, in a series of substrate/pro

61 A large family of acyl-CoA synthetases (ACSs) regulates FA metabolism by e

62 ly of proteins that includes very long-chain acyl-CoA synthetases (ACSVL) consists of six members.

63 (ACSL) and very long chain acyl-coenzyme A (acyl-CoA) synthetases [(ACSVL)/fatty acid transport prot

64 Expression of the fatty acyl-CoA synthetase ACSVL3 was found to be markedly elev

65 -chain fatty acids, and have very long-chain acyl CoA synthetase activities that were 40% wild-type.

66 The depression in very long-chain acyl CoA synthetase activities were not apparent in cell

67 ls at 24 h after infection had 2-fold higher acyl-CoA synthetase activities and 30% higher rates of f

68 al, exhibiting both fatty acid transport and acyl-CoA synthetase activities that work in concert to m

69 tty acid transport and very long-chain fatty acyl-CoA synthetase activities were distinguishable.

70 tty acid accumulation, very long-chain fatty acyl-CoA synthetase activities, and the fatty acid profi

71 on of mmFATP5 did not substantially increase acyl-CoA synthetases activities using the substrates tes

72 ximal levels of fatty-acid import and has an acyl CoA synthetase activity specific for very-long-chai

73 chain fatty acids and has reduced long-chain acyl-CoA synthetase activity (fat1Delta faa1Delta).

74 Acyl-CoA synthetase activity detected in GLUT4-enriched

75 the cytoplasm of transfected cells displayed acyl-CoA synthetase activity for long chain fatty acid s

76 FATP4 protein and acyl-CoA synthetase activity localized to multiple organ

77 The very long chain acyl-CoA synthetase activity of the two enzymes was comp

78 n either wild-type or nearly wild-type fatty acyl-CoA synthetase activity profiles; 2) those that had

79 lls revealed that most of the hsBG-dependent acyl-CoA synthetase activity was soluble and not membran

80 s, COS-1 cells expressing hsBG had increased acyl-CoA synthetase activity with either long-chain fatt

81 ACSL3 knockdown decreased total acyl-CoA synthetase activity without substantially alter

82 odulation of endoplasmic reticulum-localized acyl-CoA synthetase activity, although mutant forms of a

83 However, despite decreased acyl-CoA synthetase activity, initial rates of fatty aci

84 howed that VLCS activity, but not long-chain acyl-CoA synthetase activity, was reduced to about 40% o

85 tional in fatty acid import, only FATP2a had acyl-CoA synthetase activity, with an apparent preferenc

86 tic Acsl1 mRNA and protein levels as well as acyl-CoA synthetase activity.

87 dominant negative effect against long-chain acyl-CoA synthetase activity.

88 ntributes the majority of cardiac long-chain acyl-CoA synthetase activity.

89 converted to 5HD-CoA by mitochondrial fatty acyl CoA synthetase and acted as a weak substrate or inh

90 ssociated with genes encoding for a putative acyl-CoA synthetase and a MeaB-like chaperone.

91 esis of TAGs and CEs by targeting long-chain acyl-CoA synthetase and acyl-CoA:cholesterol acyltransfe

92 n this paper, we present evidence that FATP1/acyl-CoA synthetase and DGAT2/diacylglycerol acyltransfe

93 In contrast, adipocyte acyl-CoA synthetase and diacylglycerol acyltransferase a

94 fic activities of the key enzymes long chain acyl-CoA synthetase and diacylglycerol acyltransferase,

95 equent genetic analysis identified ACS-4, an acyl-CoA synthetase and its FA-CoA product, as key germl

96 reactions are known reactions, catalyzed by acyl-CoA synthetase and peptidylglycine alpha-amidating

97 lts indicate that FATP1 is a very long chain acyl-CoA synthetase and suggest that a potential mechani

98 AAE15 has sequence similarity to long-chain acyl-CoA synthetases and a predicted N-terminal plastidi

99 ed fatty acids are esterified to acyl-CoA by acyl-CoA synthetases and transferred to lysophospholipid

100 Triacsin C, an inhibitor of fatty acyl-CoA synthetase, and troglitazone, an enhancer of FF

101 whether adipocyte lipogenic proteins (CD36, acyl-CoA synthetases, and diacylglycerol acyltransferase

102 Because CPT-I and long-chain acyl-CoA synthetase appear to be associated with both in

103 vation in these cells, indicating that other acyl-CoA synthetases are necessary for very long-chain f

104 results show that many different AMP-forming acyl-CoA synthetases are regulated by N-lysine acetylati

105 The AMP-forming acyl coenzyme A (acyl-CoA) synthetases are a large class of enzymes found

106 n a conformation similar to those adopted by acyl-CoA synthetases as they convert acyl adenylates int

107 t did not acetylate the wild-type long-chain acyl-CoA synthetase B (RpLcsB; formerly Rpa2714) enzyme

108 Acyl-CoA synthetases belong to the superfamily of adenyl

109 The acyl:CoA synthetase blocker, triacsin C, inhibited ester

110 as palustris (RpPat) inactivates AMP-forming acyl-CoA synthetases by acetylating the epsilon-amino gr

111 M. aquaeolei VT8, to search for WS/DGAT and acyl-CoA synthetase candidate genes.

112 rence confirmed that endogenous ACSVL3 is an acyl-CoA synthetase capable of activating both long-chai

113 Short- and medium-chain acyl coenzyme A (acyl-CoA) synthetases catalyze the formation of acyl-CoA

114 Here we show that the Drosophila fatty acyl-CoA synthetase CG6178, which cannot use d-luciferin

115 onserved amino acid residues in the 12 fatty acyl-CoA synthetases compared.

116 Acyl-CoA synthetases comprise numerous proteins with div

117 lar trafficking were also found in the fatty acyl-CoA synthetase-deficient strains.

118 that PMNs and their plasma membranes use an acyl:CoA synthetase-dependent route to esterify 5-HETE a

119 This enzyme, a medium-chain acyl-CoA synthetase designated Macs(Ma), utilizes 2-meth

120 on of an FATP4 variant with mutations in the acyl-CoA synthetase domain did not provide any degree of

121 lude that expression of FATP4 with an intact acyl-CoA synthetase domain in suprabasal keratinocytes i

122 CP) synthase AasC but inhibitors of the host acyl-CoA synthetase enymes ACSL also impaired growth of

123 eudomonas palustris, at least 10 AMP-forming acyl-CoA synthetase enzymes are acetylated by the Pat ho

124 nt lysine residue in a number of FadD (fatty acyl CoA synthetase) enzymes is acetylated by KATmt in a

125 olar morphology through the long-chain fatty acyl-CoA synthetase Faa1, independently of the RNA methy

126 l for fatty acid export in cells lacking the acyl-CoA synthetases Faa1 and Faa4.

127 bolic utilization proceeds through the fatty acyl-CoA synthetases Faa1p and Faa4p.

128 These data support the hypothesis that fatty acyl-CoA synthetase (Faa1p or Faa4p) functions as a comp

129 mes acyl-CoA oxidase (POX1) and medium-chain acyl-CoA synthetase (FAA2).

130 xa2p complex functionally interacts with the acyl-CoA synthetases Faa2p and/or Fat1p on the inner sur

131 In Saccharomyces cerevisiae Fat1p and fatty acyl-CoA synthetase (FACS) are hypothesized to couple im

132 Fatty acyl-CoA synthetase (FACS, fatty acid:CoA ligase, AMP-fo

133 oA hydrolase (HIBCH, p = 8.42 x 10(-89)) and acyl-CoA synthetase family member 3 (ACSF3, p = 3.48 x 1

134 mutations in a gene encoding a member of the acyl-CoA synthetase family, a diverse group of evolution

135 ese proteins were members of the AMP-forming acyl-CoA synthetase family.

136 fatty acid transport protein/very long chain acyl-CoA synthetase (FATP/Acsvl) family are emerging as

137 ulin affects the subcellular localization of acyl-CoA synthetase FATP1.

138 in fatty acids are converted to acyl-CoAs by acyl-CoA synthetase (fatty acid CoA ligase: AMP forming,

139 Fatty acyl-CoA synthetase (fatty acid:CoA ligase, AMP-forming;

140 Saccharomyces cerevisiae contains four known acyl-CoA synthetases (fatty acid activation proteins, Fa

141 ly of AMP-forming enzymes that also includes acyl-CoA synthetases, firefly luciferase, and nonribosom

142 WLHTGDIGXWXPXGXLKIIDRKK, common to all fatty acyl-CoA synthetases for which sequence information is a

143 This region of fatty acyl-CoA synthetase from E. coli, 431NGWLHTGDIAVMDEEGFLR

144 quence information from putative WS/DGAT and acyl-CoA synthetase genes identified in this strain was

145 identified gonadotropin-regulated long chain acyl-CoA synthetase (GR-LACS) was cloned and characteriz

146 The E. coli fatty acyl-CoA synthetase has remarkable amino acid similariti

147 Enzymes catalyzing activation, acyl-CoA synthetases, have been classified by their chai

148 Fatty acid transport protein 1 (FATP1) is an acyl-CoA synthetase highly expressed in skeletal muscle

149 A human homolog of very long-chain acyl-CoA synthetase, hVLCS-H2, has two requisite propert

150 RpPat acetylated all nine of the acyl-CoA synthetases identified by this work, and RpLdaA

151 enic mouse lines that overexpress long-chain acyl-CoA synthetase in the heart (MHC-ACS).

152 hen bred to transgenic mice that overexpress acyl-CoA synthetase in the heart, a strain that exhibits

153 hus appears to function as a very long-chain acyl-CoA synthetase in wax metabolism.

154 insulin stimulation and after knocking down acyl-CoA synthetases in adipocytes.

155 While many structures exist of acyl-CoA synthetases in both conformations, to date only

156 ymatic mechanism of FadK is similar to other acyl-CoA synthetases in that it forms an acyl-AMP interm

157 ns a histidine residue where all other known acyl-CoA synthetases, including mouse and rat ACSBG2, co

158 ily of both prokaryotic and eukaryotic fatty acyl-CoA synthetases, indicating a common ancestry.

159 The long-chain acyl-CoA synthetase inhibitor triacsin C completely reve

160 Moreover, mLD formation was blocked by the acyl-CoA synthetase inhibitor triacsin C, implicating th

161 2 cell lysates with (2E)-hexadecenal and the acyl-CoA synthetase inhibitor triacsin C.

162 pression was completely blocked by the fatty acyl-CoA synthetase inhibitor, triacsin C, evidence of i

163 nation of a branched chain fatty acid and an acyl-CoA synthetase is required for critical cellular pr

164 acids to their CoA derivatives, catalyzed by acyl-CoA synthetases, is involved in AMPK activation by

165 Thus, long-chain acyl-CoA synthetase isoform 1 (ACSL1) deficiency in the

166 Long-chain acyl coenzyme A (acyl-CoA) synthetase isoform 1 (ACSL1) catalyzes the syn

167 Moreover, FATP1 and Acsl1, the 2 major acyl-CoA synthetase isoforms in adipocytes, are essentia

168 ation of fatty acids by one of 13 long-chain acyl-CoA synthetase isoforms.

169 Long-chain acyl-CoA synthetase (LACS) activities are encoded by a f

170 CS6 and LACS7, encode peroxisomal long-chain acyl-CoA synthetase (LACS) isozymes.

171 C transporter and the peroxisomal long chain acyl-CoA synthetases (LACS)6 and -7.

172 utin pathway genes, which encodes long-chain acyl-CoA synthetase LACS2, is likely to be directly targ

173 e palmitoyltransferase-I (CPT-I), long-chain acyl-CoA synthetase (LCAS), and voltage-dependent anion

174 tment increased the activities of long chain acyl-CoA synthetases (LCASs), including oleoyl-CoA synth

175 dy revealed a central role of the long-chain acyl-CoA synthetase LCS2 in the production of triacylgly

176 y CDCP1's interaction with and inhibition of acyl CoA-synthetase ligase (ACSL) activity.

177 letion of codons 350-353 in a 528-codon-long acyl-CoA synthetase-like gene (ACS).

178 ion into TAG, with long lasting increases in acyl-CoA synthetase long 1 (ACSL1) and diacylglycerol ac

179 rnitine O-palmitoyltransferase 1 (Cpt1a) and acyl-CoA synthetase long chain family member 1 (Acsl1),

180 plex consisting of TIP30, endophilin B1, and acyl-CoA synthetase long chain family member 4 (ACSL4) t

181 (Ppargc1a), uncoupling protein 1 (Ucp1) and acyl-CoA synthetase long-chain family member 1 (Acsl1),

182 s inserted within an intron of the autosomal acyl-CoA synthetase long-chain family member 3 (Acsl3) g

183 ferentiation, and that overexpression of rat acyl-CoA synthetase long-chain family member 6 (Acsl6, f

184 ependent steroid dehydrogenase-like protein, acyl-CoA synthetase, long chain family member (ACSL) 1,

185 These results suggest that although acyl-CoA synthetases may have the ability to utilize a b

186 osphate acyltransferase mRNA fell by 57% and acyl-CoA synthetase mRNA by 67% (brain isoform) and 38%

187 reported that hBG1, the human homolog of the acyl-CoA synthetase mutated in the Drosophila mutant "bu

188 allowed us to delineate a new superfamily of acyl-CoA synthetases (nucleoside diphosphate-forming) an

189 ACSL1 (acyl-CoA synthetase 1), the major acyl-CoA synthetase of adipocytes, has been proposed to

190 was significantly more similar to that of an acyl-CoA synthetase of the distantly related bacterium,

191 echanism and crystal structure evidence, the acyl-CoA synthetases, one of three subgroups of a superf

192 ACSBG1, an acyl-CoA synthetase originally identified in the fruit f

193 factor-TU, methylglutaryl coenzyme A (CoA), acyl CoA synthetase, oxoacyl CoA thiolase, and ubiquitin

194 Acyl-CoA synthetases play a pivotal role in fatty acid m

195 atty acid-CoA ligase (also called fatty acid acyl-CoA synthetase) plays an essential role in lipid bi

196 enzyme A (acyl-CoA) dehydrogenase (pltE), an acyl-CoA synthetase (pltF), a thioesterase (pltG), and t

197 ACYL-COA SYNTHETASE, POLYKETIDE SYNTHASE A (PKSA) and PK

198 rases are thought to have evolved from fatty acyl-CoA synthetases present in all insects.

199 Triacsin C, an inhibitor of fatty acyl-CoA synthetase, prevented the increase in PFK activ

200 hibition of either acetyl-CoA carboxylase or acyl-CoA synthetase reduced mineralization of CVCs, wher

201 owledge, these mutant proteins are the first acyl-CoA synthetases reported that are defective in aden

202 conserved motifs from all known families of acyl-CoA synthetases revealed that hsBG along with the D

203 cluding multiple AMP-forming CoA ligases and acyl-CoA synthetases seem to be present as ways to form

204 al cholesterol ester hydrolase 1 (NCEH1) and acyl-CoA synthetase short-chain family member 2 (ACSS2).

205 pose that this sequence represents the fatty acyl-CoA synthetase signature motif (FACS signature moti

206 e ATP/AMP binding domain and the 25-aa fatty acyl-CoA synthetase signature motif, but displays low ov

207 een site-directed mutations within the fatty acyl-CoA synthetase structural gene (fadD) corresponding

208 Evidence for homologous acyl-CoA synthetases supports a domain alternation catal

209 D cells by using triacsin C, an inhibitor of acyl-CoA synthetase that blocks the reincorporation of h

210 We report that FadK is as an acyl-CoA synthetase that has a preference for short chai

211 acid transport protein 4 (FATP4) is a fatty acyl-CoA synthetase that preferentially activates very l

212 As such, LACS1 defines a functionally novel acyl-CoA synthetase that preferentially modifies both VL

213 5 and triacsin C, an inhibitor of long chain acyl-CoA synthetase that reduces LC-CoA levels, did not

214 Acyl-CoA synthetases that activate fatty acids to their

215 rresponding sequences from other AMP-forming acyl-CoA synthetases that were known RpPat substrates.

216 eversed by triacsin C, an inhibitor of fatty acyl-CoA synthetase, the enzyme that generates LC-CoA.

217 To determine whether FATP4 is an acyl-CoA synthetase, the murine protein was engineered t

218 The adenylate-forming enzymes, including acyl-CoA synthetases, the adenylation domains of non-rib

219 studies have focused on the structure of the acyl-CoA synthetases, their post-translational modificat

220 R8/LACS1, one of nine Arabidopsis long-chain acyl-CoA synthetases thought to activate acyl chains.

221 on of Triacsin-C, an inhibitor of long-chain acyl-CoA synthetase, to AdCMV-GlpK-treated INS-1 cells d

222 e palmitoyl-CoA transferase, very long chain acyl-CoA synthetase, very long chain acyl-CoA dehydrogen

223 n crossed with animals expressing long-chain acyl-CoA synthetase via the MHC promoter (MHC-ACS), whic

224 port proteins (FATP) and the very long-chain acyl-CoA synthetases (VLACS).

225 , which each have very long-chain fatty acid acyl-CoA synthetase (VLCFA-ACS) activity, as negative re

226 d with decreased peroxisomal very long-chain acyl-CoA synthetase (VLCS) activity and decreased peroxi

227 ta-oxidation and peroxisomal very long-chain acyl-CoA synthetase (VLCS) activity.

228 previously that homolog 2 of very long-chain acyl-CoA synthetase (VLCS) can activate cholate.

229 odystrophy, are activated by very long-chain acyl-CoA synthetase (VLCS) normally found in peroxisomes

230 unction of peroxisomal very long chain fatty acyl-CoA synthetase (VLCS) that leads to severe and prog

231 tly identified family is the very long-chain acyl-CoA synthetases (VLCS).

232 Five non-acyl-CoA synthetases were identified as possibly acetyla

233 Other long- and very long-chain acyl-CoA synthetases were incapable of activating cholat

234 y (C16:0), characteristic of very long chain acyl-CoA synthetases, whereas both mutant M1 and M2 were

235 When acyl-CoA synthetase, which catalyzes the conversion of f

236 membrane-bound fatty acid transporters or as acyl-CoA synthetases, which activate long-chain fatty ac

237 ture motif) common to long- and medium-chain acyl-CoA synthetases, which appears to contribute to the

238 ed, expressed, and characterized as a 65-kDa acyl-CoA synthetase with extremely high specificity for

239 wn crystal structures of the T. thermophilus acyl-CoA synthetase with remarkably high levels of conse

240 dings indicate that inhibition of long-chain acyl-CoA synthetases with triacsin C, a fatty acid analo

241 The ydiQRSTD operon encodes a putative acyl-CoA synthetase, ydiD (renamed fadK), as well as put