ライフサイエンスコーパス: adaptin

1 proteins colocalized with clathrin and alpha-adaptin.

2 N-terminal trunk domains of gamma- and beta1-adaptin.

3 tions of a transmembrane receptor with beta2-adaptin.

4 mplex formation with Rab11, Reps1, and alpha-adaptin.

5 by expression of a dominant negative micro1-adaptin.

6 or, SH3 domains, phosphoinositides, and beta-adaptin.

7 stin-2 interaction with clathrin and beta(2)-adaptin.

8 ed with Golgi-specific 58K protein and gamma-adaptin.

9 logy to the C-terminal "ear" domain of gamma-adaptin.

10 its binding to the appendage domain of alpha-adaptin.

11 bunits of this complex, which we call beta3A-adaptin.

12 nce of cell fate determinants Numb and alpha-Adaptin.

13 ing site for the Nef diacidic motif on alpha-adaptin.

14 milar endocytic roles, e.g., beta1 and beta2-adaptin.

15 of polarized MDCK cells independent of mu1B adaptin.

16 tion of extrasynaptic receptors with the AP2 adaptin.

17 x, as determined by coprecipitation of alpha-adaptin.

18 in xARH abolished binding to alpha- and beta-adaptins.

19 eterotetrameric assemblies of subunits named adaptins.

20 mutation of AP-1, but not AP-2, AP-3, or GGA adaptins.

21 structure, and pseudogenes for the different adaptins.

22 ins, which contain domains homologous to the adaptins.

23 ion with homology to the ear domain of gamma-adaptins.

24 similar to beta-NAP and to beta1- and beta2-adaptins.

25 1 but continues to exhibit association with adaptin 2 alpha and beta subunits.

26 ion and cell chemotaxis and imply a role for adaptin 2 in the endocytosis of CXCR2.

27 -arrestin 1 but exhibit decreased binding to adaptin 2alpha and beta.

28 plex-2 (BLOC-2) (HPS-5), BLOC-3 (HPS-1), and adaptin-3 (HPS-2).

29 es lysosomes and related organelles, whereas adaptin-3 is responsible for trafficking among these org

30 ted in these cells and in cells depleted for adaptin-3 or endoplasmic reticulum membrane complex subu

31 ndependent strains with depleted V-ATPase or adaptin-3 subunits were isometamidium resistant, and che

32 en identified 14 V-ATPase subunits and all 4 adaptin-3 subunits, implicating acidic compartment defec

33 A/MART-1, LAMP2, Rab 27, transferrin, c-kit, adaptin-3, and the HPS1 protein) appeared normal.

34 ated colocalization of E-selectin with alpha-adaptin, a clathrin-associated protein.

35 roscopy demonstrated colocalization of alpha-adaptin, a component of clathrin-coated pits, with wild-

36 caused monocyte TF to colocalize with alpha-adaptin, a component of clathrin-coated pits.

37 I3K-C2alpha closely paralleled that of gamma-adaptin, a component of the AP-1 adaptor that is present

38 specifically coimmunoprecipitated with alpha-adaptin, a component of the AP-2 complex that interacts

39 first identified a human cDNA encoding delta-adaptin, a structural homolog of the alpha- and gamma-ad

40 apses and decreased binding of NR2B to beta2-adaptin, a subunit of AP-2, thus blocking the activity-d

41 wn to be colocalized with clathrin and alpha-adaptin, a subunit of the AP2 adaptor protein which link

42 ction between Dishevelled2 (Dvl2) and micro2-adaptin, a subunit of the clathrin adaptor AP-2; this in

43 relates with increased interaction with beta-adaptin, a subunit of the clathrin adaptor protein compl

44 tyrosine motif that serves as the signal for adaptin alpha and a dileucine motif that serves as the s

45 In vivo coimmunoprecipitation confirmed adaptin alpha and adaptin gamma complexes, but adaptor p

46 ey contain distinct binding motifs for alpha-adaptin (alpha-Ada) and proteins with Eps15 homology (EH

47 Here we show that alpha-Adaptin (alpha-Ada), a subunit of the endocytic AP-2 com

48 n of endocytic proteins CIMPR, clathrin, and adaptin-alpha, and LGMD2B muscle exhibited decreased exp

49 rminal region of arrestin-2 mediated beta(2)-adaptin and clathrin interaction with Phe-391 and Arg-39

50 as significantly enhanced binding to beta(2)-adaptin and clathrin.

51 NA encoding the Drosophila ortholog of delta-adaptin and found that transcripts specified by this cDN

52 stin1 Tyr-54 lessens the interaction with mu-adaptin and moreover is a Src phosphorylation site.

53 Specifically, we show that alpha-adaptin and Rab11, which function critically in the endo

54 Berdnik et al. show that Numb recruits alpha-Adaptin and that this physical interaction plays a role

55 both the beta-arrestin1 interaction with mu-adaptin and the ability to enhance beta2-adrenergic rece

56 ctural and evolutionary relationships of the adaptins and the genetic analyses of their function.

57 tion of endocytosis proteins, dynamin, alpha-adaptin, and clathrin.

58 Kir2.3 co-immunoprecipitates with alpha adaptin, and disruption of the di-isoleucine motif decre

59 uced colocalization of E-selectin with alpha-adaptin, and inhibited E-selectin-mediated neutrophil ro

60 um dependent, inhibited by SNAP-25 and alpha-adaptin, and results in the inhibition of receptor-media

61 ptor internalization and bind clathrin, beta-adaptin, and Src to comparable levels as wild type arres

62 of neutropenia genes to include WASp, Gfi-1, adaptin, and tafazzin.

63 bodies directed against opsin, arrestin, and adaptin, and they have a large distribution of sizes, av

64 erference-mediated knockdown of either alpha adaptin (AP-2 clathrin adaptor) or clathrin heavy chain,

65 spatial extent was regulated by the clathrin adaptin AP180.

66 the functional analysis of redundant AP-1 mu-adaptins AP1M1 (also known as muB1) and AP1M2 (also know

67 preserved, and the recruitment of the beta2-adaptin, AP2 adaptor complex to clathrin as well as tran

68 e identify dynamin and the EAP-binding alpha-adaptin appendage domain of the AP2 adaptor as switches

69 ic motif on Nef and the basic patch on alpha-adaptin are both required for the cooperative assembly o

70 Adaptins are subunits of adaptor protein (AP) complexes

71 clathrin (arrestin-2-DeltaLIELD) or beta(2)-adaptin (arrestin-2-F391A) interaction.

72 ata indicate that beta-arrestin2 utilizes mu-adaptin as an endocytic partner, and that the inability

73 Arg-395 having an essential role in beta(2)-adaptin binding and LIELD (residues 376-380) having an e

74 tants defective in both clathrin and beta(2)-adaptin binding functioned as effective dominant negativ

75 manner, without effects on clathrin or gamma-adaptin binding.

76 -arrestin1 and -2 differentially regulate mu-adaptin binding.

77 trapeptide YDSI, a perfect fit to the YXXPhi adaptin-binding consensus.

78 s 4 armadillo repeats, and to the N-terminal adaptin-binding domain in mu2 from positions 1 to 145.

79 Furthermore, we identify a dileucine AP2 adaptin-binding motif within the receptor beta2 subunit

80 alpha- and gamma-adaptin-binding protein p34, a cytosolic protein with a

81 utations in AAGAB, encoding alpha- and gamma-adaptin-binding protein p34, located at a previously lin

82 beta2-Adaptin binds betaarrestin 2 in a yeast two-hybrid assay

83 rescued by transfection with wild-type delta-adaptin but not by delta-adaptin containing mutations th

84 ion of cell fate determinants Numb and alpha-adaptin by confocal microscopy were used to assess frequ

85 ge of migrating cells and depletion of beta2-adaptin by RNAi increases cell spreading and inhibits di

86 uated using mutant constructs of the beta(2)-adaptin C terminus containing either the clathrin and th

87 The absence of delta-adaptin causes destabilization of the AP3 complex in mou

88 abeled for annexins I, II, IV, and VI; alpha-adaptin; clathrin heavy chain; or beta-coatomer protein.

89 The T. gondii AP-1 adaptin complex is implicated in this transport because

90 In contrast, beta3, beta4, and all other adaptin complex subunits, as well as paralogues of the s

91 IFNAR1 promotes its interaction with the AP2 adaptin complex that is required for the robust internal

92 nformation (colocalized with gE and the AP-1 adaptin complex).

93 family mediates suramin uptake, and the AP1 adaptin complex, lysosomal proteases and major lysosomal

94 es and in trans through mutation of the AP-3 adaptin complex; both cis- and trans-mutations result in

95 ocytosis of transferrin receptors, a beta(2)-adaptin construct capable of associating with beta-arres

96 In addition, although the beta(2)-adaptin construct containing both the clathrin and beta-

97 ith wild-type delta-adaptin but not by delta-adaptin containing mutations that abolish VAMP7 binding,

98 ecipitation analyses demonstrate that beta3A-adaptin corresponds to the approximately 140-kDa subunit

99 otein trafficking toward the PVs is clathrin-adaptin dependent.

100 ndocytic organelle to mature rhoptries in an adaptin-dependent fashion.

101 Mutation of a putative AP2 adaptin dileucine motif (L314A, L315A) present in the re

102 he first report to our knowledge that an AP2 adaptin dileucine recognition motif is critical for the

103 nents of the AP-2 complex (alpha- and beta 2-adaptin) during clathrin-mediated endocytosis.

104 moted apical accumulation of clathrin, alpha-adaptin, dynamin, and F-actin and increased the amounts

105 the endocytic syntaxins, Rab 5, and the beta-adaptins each reveal a pattern of ancestral, undifferent

106 The carboxyl-terminal ear domains (or gamma-adaptin ear (GAE) domains) of two gamma-adaptin subunit

107 (VHS); GGAs and TOM1 (GAT); hinge; and gamma-adaptin ear (GAE).

108 have identified the golgi-associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1) as

109 (alpha2-ARs) by GGA3 (Golgi-localized, gamma-adaptin ear domain homology, ADP ribosylation factor-bin

110 etermined the role of Golgi-localized, gamma-adaptin ear domain homology, ADP ribosylation factor-bin

111 clathrin-associated Golgi-localizing, gamma-adaptin ear domain homology, ARF-binding proteins (GGA).

112 us identify a binding sequence for GAE/gamma-adaptin ear domains and reveal a functional link between

113 of expressed GGA1myc (Golgi-localizing gamma-adaptin ear homology ARF-binding protein) but with no si

114 agenesis of the LR11 Golgi-localizing, gamma-adaptin ear homology domain, ADP-ribosylation factor (GG

115 The GGAs (Golgi-localizing, gamma-adaptin ear homology domain, ARF-binding proteins) are a

116 eins have been named Golgi-localizing, gamma-adaptin ear homology domain, ARF-binding proteins, or GG

117 The GGAs (Golgi-localizing, gamma-adaptin ear homology domain, ARF-binding) are a family o

118 The GGAs (Golgi-localizing, gamma-adaptin ear homology domain, ARF-binding) are a multidom

119 athrin adaptors, Gga (Golgi-localized, gamma-adaptin ear homology, Arf-binding) proteins and the AP-1

120 g hinge segment, and a C-terminal GAE (gamma-adaptin ear) domain.

121 or activity requires hRME-6 binding to alpha-adaptin ear, which displaces the ear-associated mu2 kina

122 se subunit II (COX2), Golgi-associated gamma adaptin ear-containing ARF binding protein 1 (GGA1), pol

123 The Golgi-localized, gamma-adaptin ear-containing, ARF-binding (GGA) proteins are m

124 n 1 (AP-1), AP-3, and Golgi-localized, gamma adaptin ear-containing, Arf-binding (GGAs) protein.

125 e we demonstrate that Golgi-localized, gamma adaptin-ear-containing ADP ribosylation factor-binding p

126 ce also recognized by the gamma1- and gamma2-adaptin ears), while GGA-GAT domains bind to the C-termi

127 ticle, we report the results of a survey for adaptins from sequenced genomes including those of man,

128 tion of macrophages or mice, indicating that adaptin function is crucial for pathogenesis in these un

129 unoprecipitation confirmed adaptin alpha and adaptin gamma complexes, but adaptor protein 180 complex

130 ileucine motif that serves as the signal for adaptin gamma.

131 Generation of sigma 1- and mu 1-adaptin gene deletion mutants shows that these promastig

132 ng and characterization of sigma 1- and mu 1-adaptin gene homologues from the eukaryotic protozoan pa

133 equence comparisons also suggest that beta3A-adaptin has a domain organization similar to beta-NAP an

134 r, the beta-arrestin-binding site in beta(2)-adaptin has not been identified, and little is known abo

135 NLD clathrin box motif derived from the beta-adaptin hinge region.

136 itro with beta-NAP, a neuronal-specific beta-adaptin homolog that was identified as an autoantigen in

137 The Golgi-associated, gamma-adaptin homologous, ADP-ribosylation factor (ARF)-intera

138 Knockdown of beta2-adaptin in both U2OS osteosarcoma cells and MCF10A norma

139 not truncated, pp120 co-localized with alpha-adaptin in the adaptor protein complex that anchors endo

140 describing the association of ATM with beta-adaptin in vesicles indicate that ATM may play a role in

141 at it directly binds the ear domain of alpha-adaptin in vivo and in vitro.

142 a complex containing Src, dynamin, and alpha-adaptin indicates that Src may play a more general role

143 n 2 minigene constructs containing the beta2-adaptin interacting region inhibits beta2AR endocytosis.

144 n of Numb, but not mutant Numb lacking alpha-adaptin-interacting domain, leads to accumulation of Not

145 -adrenergic receptor, while arrestin/beta(2)-adaptin interaction appeared less critical.

146 t-negative mutants lacking clathrin- or beta-adaptin interaction sites fails to block GPER internaliz

147 Biochemical analyses demonstrated that delta-adaptin is a component of the adaptor-like complex AP-3

148 Beta2-adaptin is a subunit of the AP-2 complex and is involved

149 Furthermore, beta2-adaptin is enriched in adhesions at the leading edge of

150 beta3A-adaptin is expressed in all tissues and cells examined.

151 beta3A-adaptin is not present in significant amounts in clathri

152 beta3A-adaptin is phosphorylated on serine residues in vivo whi

153 Herein, beta2-adaptin is shown to bind to the focal adhesion protein a

154 of AP complexes arises from the existence of adaptin isoforms encoded by distinct genes or resulting

155 Rab11 and alpha-adaptin localization occurs in the absence of a polarize

156 s cell polarity and migration and that beta2-adaptin may control the balance between the formation of

157 tain a similar degree of interaction with mu-adaptin may result from coordination of Tyr-54 by neighb

158 signals have a well-appreciated role in the adaptin-mediated sorting of mammalian cells, this is the

159 In trypanosomatids, the adaptin-mediated sorting of proteins is largely uncharac

160 ly prevents the interaction of Env with host adaptin molecules that recruit plasma membrane molecules

161 CD63, clathrin heavy chain, and beta-adaptin mRNAs, all of which encode proteins associated w

162 alpha-adaptin mutants are viable and the remaining mu2/beta he

163 ed by a direct interaction between the beta2-adaptin N-terminal trunk domain and the cytoplasmic tail

164 1 are complexed with the large subunit gamma-adaptin of AP-1.

165 Because of uncertain homology, the mu-adaptins of Arabidopsis have been designated muA through

166 a telangiectasia mutated (ATM) binds to beta-adaptin, one of the components of the AP-2 adaptor compl

167 e interaction with endocytic (clathrin, beta-adaptin) or signaling (Src) components and is in contras

168 AP-3 complex, the other subunits being delta-adaptin, p47A (now called mu3A) and sigma3 (A or B).

169 in the unc-11 gene, which encodes a clathrin adaptin protein (AP180).

170 ith linear endocytic motifs that recruit the adaptin protein complex 2 (AP2)-clathrin molecules, mono

171 NAR1 from interacting with components of the adaptin protein complex 2 (AP2).

172 Knockdown of clathrin adaptin protein complex-1 (AP-1) causes a delay in the r

173 AP1S2 encodes an adaptin protein that constitutes part of the adaptor pro

174 in double mutants lacking both the clathrin adaptin protein unc-11 AP180 and eat-4 VGLUT.

175 ic motif within IFNAR1 to recruitment of the adaptin protein-2 complex (AP2) and ensuing receptor end

176 The Golgi-associated gamma-adaptin-related ADP-ribosylation factor-binding proteins

177 Finally, we extend our survey to adaptin-related proteins such as the GGAs and stonins, w

178 photoinactivation blocked Clathrin and alpha-adaptin relocalization to synaptic membranes upon nerve

179 The characteristics of beta3A-adaptin reported here lend support to the idea that AP-3

180 he interaction of beta-arrestin with beta(2)-adaptin represents a selective endocytic trigger for sev

181 The binding of VAMP7 to delta-adaptin requires the VAMP7 SNARE motif to be engaged in

182 The predicted amino acid sequence of beta3A-adaptin reveals that the protein is closely related to t

183 could associate with both dynamin and alpha-adaptin simultaneously, further supporting a role for am

184 wt hearts had fewer cells with uniform alpha-adaptin staining indicative of symmetrically dividing CP

185 2 is mediated by the ear domain of its alpha-adaptin subunit and binding of clathrin by the amino-ter

186 ptor protein complex-2 (AP-2), where the mu2-adaptin subunit binds directly to a tyrosine-based motif

187 amma-adaptin ear (GAE) domains) of two gamma-adaptin subunit isoforms of AP-1 and of the GGAs are str

188 and ARF6 interacted directly with the beta1-adaptin subunit of AP-1 in the presence of GTPgammaS.

189 ow that Rab11-FIP2 associates with the alpha-adaptin subunit of AP-2 complexes, which are known to re

190 trating specific colocalization of the alpha adaptin subunit of AP-2 with the EIAV p9 protein at site

191 he protein bind to clathrin and to the beta2-adaptin subunit of AP-2.

192 e C-terminal region of ARH bound the beta(2)-adaptin subunit of AP-2.

193 aining a GPCR, betaarrestin 2, and the beta2-adaptin subunit of AP-2.

194 coat-associated protein that binds the alpha-adaptin subunit of AP-2.

195 tin motifs recognizing clathrin and the beta-adaptin subunit of AP2.

196 hosphorylation and phosphorylating the beta2-adaptin subunit of AP2.

197 We show that the linker of the delta-adaptin subunit of AP3 binds the VAMP7 longin domain and

198 eptor (GPCR) activation, bind to the beta(2)-adaptin subunit of the clathrin adaptor AP-2.

199 homology (EH) domain of Eps15 and the alpha-adaptin subunit of the clathrin adaptor AP-2.

200 bound directly and specifically to the beta-adaptin subunit of the clathrin adaptor complexes AP-1 a

201 th an Arabidopsis (Arabidopsis thaliana) muA-adaptin subunit.

202 antly decreased the phosphorylation of large adaptin subunits and the normally punctate AP-2 distribu

203 nteracted directly with the beta3- and delta-adaptin subunits of AP-3.

204 a structural homolog of the alpha- and gamma-adaptin subunits of the clathrin coat adaptors AP-1 and

205 re distantly related to the beta1- and beta2-adaptin subunits of the clathrin-associated adaptor comp

206 om embryo extracts including alpha- and beta-adaptins, subunits of the AP-2 endocytic complex.

207 duced interaction between arrestin and beta2-adaptin, suggesting that the mutation impairs a property

208 te residues (Glu-849 and Glu-902) in beta(2)-adaptin that are important in beta-arrestin binding.

209 d residue in the appendage domain of beta(2)-adaptin that is required for interaction with the adapte

210 mplex contains a specific medium subunit, mu-adaptin, that selects cargo proteins bearing sequence-sp

211 arrestin interaction with clathrin, beta(2)-adaptin (the beta-subunit of the adaptor protein AP2), a

212 on, which requires arrestin binding to beta2-adaptin (the beta2 subunit of the clathrin-associated ad

213 beta-arrestin 2 to clathrin heavy chain/beta-adaptin, thereby accelerating receptor internalization.

214 For beta-adaptins, this fragment binds to cytoplasmic di-leucine-

215 th in vivo and in vitro the ability of beta2-adaptin to directly couple TGF-beta receptors to AP2 and

216 We complete the assignment of adaptins to AP complexes and provide information on the

217 AP-2 complex, via an interaction with beta2-adaptin, to focal adhesions mediates cell polarity and m

218 Moreover, beta2-adaptin translocates from the cytosol to the plasma memb

219 s, lysine-297 and arginine-340, on the alpha-adaptin trunk domain.

220 domain of synaptotagmin binds mu2- and alpha-adaptin, two of the four subunits of the endocytic adapt

221 Genetic analysis of cdk-5 and the clathrin adaptin unc-11 AP180 suggests that CDK-5 functions prior

222 Genetic analysis of klp-4 and the clathrin adaptin unc-11/AP180 suggests that klp-4 functions befor

223 dynamin, the clathrin adaptor protein alpha-adaptin was also found.

224 The interaction between ATM and beta-adaptin was confirmed in vitro, and coimmunoprecipitatio

225 examined after shearing, clathrin and alpha-adaptin were colocalized with beta PP on the surface tha

226 tform subdomain of the C terminus of beta(2)-adaptin, where accessory/adapter endocytic proteins for

227 es its binding to the alpha c subunit of AP2 adaptin, which is also concentrated in nerve terminals.

228 The interaction of beta(2)-adaptin with beta-arrestin involves critical arginine re