ライフサイエンスコーパス: adducted

1 reas motif-poor sequences were only sparsely adducted.

2 nonactivity measurement expressed as percent adducted.

3 The thermal destablization of the adducted 12-mers ranged from 5 to 16 degrees C and is de

4 The alkali-adducted 18-crown-6 ether ions, including (M + Li)(+), (

5 ated mice had significant extents of protein-adducted 18:1-NO2 detected by exchange to added beta-mer

6 yielded diastereomeric pairs of diol epoxide-adducted 2'-deoxyadenosine (dA) and 2'-deoxyguanosine (d

7 across from them, but among mismatches the (adducted A*) x A mispair is the most favored for extensi

8 dent brain soluble lysates is preferentially adducted, adduction is stereospecific with respect to th

9 o block the Watson-Crick base pairing of the adducted adenine with thymine, potentially contributing

10 rog, and 2.5 +/- 0.42 microg of acetaldehyde-adducted albumin, respectively, during a 4-hour period.

11 itation demonstrated that the ligand becomes adducted almost exclusively to transmembrane domains (TM

12 igestion of the enzyme, and isolation of the adducted amino acid residues, these adducts were identif

13 the platelet proteins, and isolation of the adducted amino acid residues, this adduct was identified

14 Subsequent spontaneous hydrolysis of the adducted amino acid yields bis(levuglandinyl) urea and t

15 chloride mixture was introduced, all alkali-adducted analyte ions were simultaneously detected.

16 e solvent accessibility and local pKa of the adducted and neighboring amino acids contribute to the s

17 r polymerase appears to discriminate between adducted and nonadducted DNA substrate for binding.

18 ymine dimer, as well as acetyl aminofluorene adducted- and cisplatinated-guanine residues.

19 tension assays in which the template DNA was adducted at a single adenine by either R- or S-enantiome

20 by T7 RNA polymerase past an adenine residue adducted at the N6-position with (-)-anti-trans- or (+)-

21 In the minor conformer, the adducted base assumes an anti-glycosidic torsion angle w

22 N2-ethyl-Gua at the active site reveals the adducted base in the syn configuration when the correct

23 a minor groove DNA adduct by positioning the adducted base in the syn configuration.

24 Buckling of the adducted base pair as well as the C(5)-G(18) base pair t

25 the NER gap synthesis, when encountering an adducted base present in the ICL repair intermediate, ca

26 Anti to syn rotation of the adducted base, consistent with previous NMR data for a B

27 quired for XPD cross-linking to the psoralen-adducted base, neither XPA nor XPC is cross-linked to th

28 ation frequency and spectrum produced by the adducted base.

29 the base 3' to the lesion is the same as the adducted base.

30 oxynucleotides were synthesized in which the adducted bases were centrally located within the context

31 ession of NASH, mice were immunized with MDA-adducted bovine serum albumin (MDA-BSA) before feeding t

32 unadducted BuChE and GB-, GF-, VX-, and RVX-adducted BuChE.

33 cant plasma immunoreactivity to not only MAA-adducted but also unmodified rat liver proteins (cytosol

34 racterization and quantification of proteins adducted by 2-alkenals derived from lipid peroxidation (

35 x histidine residues of Mb that were readily adducted by HNE, including the proximal (HIS 93) and dis

36 ce of circulating antibodies against protein adducted by lipid peroxidation products.

37 used to locate "twin" ion peaks of peptides adducted by NAPQI and for shotgun proteomics via tandem

38 An increase in melting temperature of CT DNA adducted by the platinum compounds is observed at low sa

39 strains based on detection of liver proteins adducted by the reactive metabolite.

40 identity of the amino acid residue that was adducted can be established, which eliminates the need f

41 By contrast, ECD of Ca-, Mn-, Zn-, and Fe-adducted CCKS and ECD of deprotonated GST with two calci

42 vior observed in ECD of protonated and metal-adducted CCKS and GST, respectively, is proposed to be a

43 We show that BPDE adducted codon 157 has greater structural distortion tha

44 R1, enable unequivocal identification of CPM-adducted cysteines.

45 Consequently, BQ-adducted cytochrome c fails to initiate caspase-3 activa

46 e participate in the error-free bypass of DE-adducted dA in vivo by predominantly incorporating dTMP

47 the intercalation site especially at the 10R adducted dA, such that this base interconverts between t

48 ate into DNA on the 3' or the 5' side of the adducted deoxyadenosine for the 1S- and 1R-adducts, resp

49 leotide binding pocket is distorted, and the adducted deoxyguanosine is in a syn conformation, exposi

50 The assay involves ligating an adducted deoxyoligonucleotide into supF containing pSP18

51 The adducted dG maintains the anti-conformation about the gl

52 ite respectively, (+)-anti-trans-(C10S) BPDE-adducted DNA alone permitted the formation of full-lengt

53 Adducted DNA cross-linked to Arg-Trp-Arg-Arg and Lys-Trp

54 e disruption to the interactions between the adducted DNA duplex and the polymerase.

55 vitro top1 inhibition results obtained with adducted DNA duplexes.

56 structures of its complexes with native and adducted DNA have been analyzed in considerable detail.

57 d that the reduced catalytic efficiency with adducted DNA involved both an increased Km and attenuate

58 reased affinity of topoisomerase IIalpha for adducted DNA or a decreased rate of religation.

59 Error-prone pol V copied G-BaP DE-adducted DNA poorly, forming A*G-BaP DE-S and -R mismatc

60 the interaction of DNA binding proteins with adducted DNA substrates.

61 ary Pol.DNA.dNTP complexes between MeFapy-dG-adducted DNA template:primer duplexes and the Y-family p

62 l-free conditions tomamycin- or saframycin-A-adducted DNA templates inhibited DNA synthesis similarly

63 d, even though binding of RPA-XPA complex to adducted DNA was observed, the presence of RPA had littl

64 Titration of the BPDE-adducted DNA with UvrA was used to generate binding isot

65 ite by aligning with the major groove of the adducted DNA within the ternary complex of polymerase an

66 or greater than that measured on fluorescein-adducted DNA, an excellent substrate for UvrABC.

67 trategy for recognition and amplification of adducted DNA, and adduct-templated incorporation of an a

68 eaction, with either UV-damaged or cisplatin-adducted DNA.

69 polymerase in human cells, acts on acrolein-adducted DNA.

70 odium cyanoborohydride, and pre-reduction of adducted DNAs inhibited complex formation.

71 elix at BaP DE-dA adduct sites may make such adducted DNAs more susceptible to the action of helicase

72 The mutation frequencies for the adducted DNAs were at least 10 times higher than for the

73 adducts and the subsequent resection of Top2-adducted DSB ends.

74 DSB ends, is required for resection of Top2-adducted DSBs, as well as for cellular resistance to eto

75 mationally stable than the corresponding 10S adducted duplex corresponding to trans opening of the ca

76 Incubation of the same adducted duplex with Chinese hamster ovary cell-free ext

77 we observe energy transfer from the virus to adducted dye molecules, resulting in a highly fluorescen

78 , 608 and 623 [M-H]- consistent with B[a]PDE adducted (either on the base or phosphate group) 2'-deox

79 EID of Mn(II)-adducted fatty acids allowed determination of all double

80 emote fragmentation patterns of alkali metal-adducted fatty acids following high energy collision act

81 Here, we analyzed metal-adducted fatty acids to investigate the utility of elect

82 For Mn(II)-adducted fatty acids, reduced characteristic charge-remo

83 ue to form with high yield-albeit in heavily adducted form.

84 ree selected series of mono-, bis-, and tris-adducted fullerene materials.

85 he rate of incorporation of C opposite a non-adducted G.

86 6 were detected following replication of the adducted genomes in wild-type E. coli strains.

87 ally incorporated into M13MB102 DNA, and the adducted genomes were electroporated into wild-type or m

88 The disulfide-adducted glutathione in the TTase-SSG complex has an ext

89 ations on the interaction energy between the adducted glutathionyl moiety and GRx were roughly estima

90 SI) fragmentation spectra derived from anion-adducted glycans were evaluated for structural determina

91 relieves repulsive interactions between the adducted guanine base and the triphosphate of the oxidiz

92 yielded ensembles of structures in which the adducted guanine was displaced into the major groove wit

93 minated cytosine (i.e., uracil) opposite the adducted guanine was dramatically decreased at the Pol b

94 rine nucleotides (but not dTMP) opposite the adducted guanine was observed.

95 DNA adducts such as N-2-acetylaminofluorene-adducted guanine, (+)- and (-)-trans-anti-benzo[a]pyrene

96 nefficiently opposite an acetylaminofluorene-adducted guanine, but was unresponsive to a template TT

97 Opposite an AAF-adducted guanine, human Pol iota was able to incorporate

98 y linked to G4(N7) and is oriented 3' to the adducted guanine.

99 ed a G opposite an acetylaminofluorene (AAF)-adducted guanine.

100 uences containing an A, G, or T opposite the adducted guanine.

101 bles it to incorporate a C opposite the N(2)-adducted guanines in DNA.

102 oficient and error-free synthesis through N2-adducted guanines that obstruct replication.

103 in morphology and inferred function, with an adducted hallux, an elongated tarsus, and derived ankle

104 n human-like foot anatomy, with a relatively adducted hallux, medial longitudinal arch, and medial we

105 ich were accompanied by the formation of tBA-adducted heme products.

106 report CD and UV-Vis spectroscopy studies of adducted human Hb that revealed loss of alpha-helical co

107 the helix, but not hydrogen bonded with the adducted inosine.

108 s provides the opportunity to produce cation-adducted intact molecular ions of sugars.

109 served in the mass spectra as [Sb(2)-tar(2)]-adducted ionic species.

110 Relative intensity dependence of these adducted ions was monitored as the volume composition of

111 Sulfate adducted ions were observed in the product ion spectra o

112 nsity of {[Sb(2)-tar(2)][H-O-CH(2)-O-H]}(2-) adducted ions.

113 G-BaP DE-S-adducted M13 DNA yielded 4-fold fewer plaques when trans

114 Adducted Mb promoted lipid oxidation in washed muscle mo

115 n in washed muscle more effectively than non-adducted Mb.

116 Covalently adducted MTS ethylammonium(+) (MTSEA(+)) strongly increa

117 followed by CD spectroscopy of the isolated adducted nucleosides indicated that, in both cases, the

118 The adducted oligodeoxynucleotides were sensitive to hot pip

119 e intrastrand cross-links, site specifically adducted oligodeoxynucleotides were synthesized in which

120 Structure proof of the adducted oligonucleotide 26 was provided by enzymatic di

121 removed by treatment with ZnBr2 to give the adducted oligonucleotide 26.

122 ) mass spectrometry to examine the cisplatin-adducted oligonucleotide and its interaction with the hu

123 or the preparation of polycyclic hydrocarbon adducted oligonucleotide building blocks.

124 The adducted oligonucleotide complements with itself, retain

125 Preferential binding of a fluorescein-adducted oligonucleotide was observed, and XPD helicase

126 ith 4-fold greater incision activity on BPDE-adducted oligonucleotides and 1.5-fold greater on [(3)H]

127 ificantly decreased affinity of T7- with the adducted oligonucleotides or the dNTP.

128 The adducted oligonucleotides were incorporated into plasmid

129 Both the nonadducted and BPDE-adducted oligonucleotides were introduced into two doubl

130 g activity remains unaffected in N2-ethyl-dG adducted oligonucleotides.

131 examined range of electron energy, the metal-adducted oligosaccharide exhibited several fragmentation

132 Our results demonstrate that ECD of metal-adducted oligosaccharides is a valuable tool for structu

133 ectron activated dissociation (ExD) of metal-adducted oligosaccharides were explored, using permethyl

134 For all metal-adducted oligosaccharides, complementary structural info

135 We show that, for certain metal-adducted oligosaccharides, particularly maltoheptaose, c

136 Rho-specific GEF) cannot exchange the 6-TGDP adducted on Rac1 with free guanine nucleotide.

137 quence is human-like and the hallux is fully adducted, other intrinsic proportions and pedal features

138 lution phase method to produce the gas-phase adducted PC anion is also demonstrated.

139 3A4-[(3)H]ritonavir incubations exhibited an adducted peptide (255-RM K: ESRLEDTQKHR-268) associated

140 oportional to the serum concentration of MeP-adducted peptide (MeP-P) over the nominal concentration

141 SALSA provides superior detection of adducted peptides compared to conventional tandem MS pre

142 ipitated from transgenic hearts revealed GSH-adducted peptides corresponding to adenine nucleotide tr

143 cal significance; amino acid mutations; salt-adducted peptides in a sample that has nominally been de

144 Isolation of CPM-adducted peptides is achieved by analytical and microbor

145 Tandem mass spectrometry of Cu-adducted phospholipids was also performed for comparison

146 rared multiphoton dissociation (IRMPD) of Ag-adducted phospholipids were investigated as structural t

147 CAD and IRMPD of Ag-adducted phospholipids with unsaturated fatty acids (R(x

148 d UvrABC(Tma) specifically incised both BPDE-adducted plasmid DNAs and site-specifically modified 50-

149 cleotides and 1.5-fold greater on [(3)H]BPDE-adducted plasmid DNAs.

150 prepare a series of mass spectra of ammonium-adducted polypropylene glycol (PPG) with an average mole

151 wever, at much lower concentrations, an IsoK-adducted protein (ovalbumin) and peptide (Ab1-40) signif

152 aR(-/-) mice immunized with 4-hydroxynonenal-adducted protein developed severe retinal impairment unr

153 No change in total HNE-adducted protein was observed between regions or stages.

154 r thinning, and increased methylglyoxal (MG)-adducted protein.

155 , rather than special characteristics of the adducted protein.

156 In contrast, under CIU conditions, the metal-adducted proteins are markedly more compact than their m

157 Because adducted proteins are not rapidly cleared, IsoK/LG prote

158 However, sulfur-nitrogen linked adducted proteins are often difficult to detect because

159 usly shown that hydralazine attacks carbonyl-adducted proteins in an "adduct-trapping" reaction that

160 cant increase in antibody titers against MAA-adducted proteins was observed in the ethanol-fed animal

161 Five abundant adducted proteins were identified as galectin-1, protein

162 at by increasing the steric bulk of carbonyl-adducted proteins, adduct-trapping drugs trigger the int

163 that by increasing the bulkiness of acrolein-adducted proteins, adduct-trapping might alter the redis

164 against sequence data bases to identify the adducted proteins.

165 gel electrophoresis before detection of HNE-adducted proteins.

166 of protein extracts and the low abundance of adducted proteins.

167 he ability of the proteasome to degrade IsoK-adducted proteins/peptides and the effect of IsoK and Is

168 ins/peptides and the effect of IsoK and IsoK-adducted proteins/peptides on proteasome function.

169 adopt a single charge state, suggesting that adducted protons were located on every other monomer uni

170 Several adducted residues in those proteins were located in conf

171 The adducted residues were determined by liquid chromatograp

172 The CPM-adducted RyR1 is separated by gel electrophoresis and su

173 mutations were completely abolished when the adducted shuttle vector was replicated in cells lacking

174 r-prone, resulting in mutations opposite the adducted site or at the +1 position from the adduct and

175 the 5th and 6th phosphates 3' to the protein-adducted site, generating as a major product of the reac

176 Major drug-adducted sites identified by repetitive primer extension

177 n the p53 cDNA through the conversion of the adducted sites into single strand breaks and sequencing

178 Only G --> T mutations were detected at the adducted sites; the 10S adduct derived from the highly c

179 The identity of the adducted species, including products such as H(2)O(2), N

180 and the possible presence of metal or other adducted species.

181 e DNA, resulting in only 60% cleavage of the adducted strand and 75% cleavage of the complementary st

182 oss of the DB[a,l]P moiety from the original adducted strand to give an 11-mer with an unmodified cen

183 drocarbon is much less severe in the present adducted structure than its cis-opened diol epoxide coun

184 was similar with (+)-trans- and (+)-cis-BPDE-adducted substrates, suggesting that UvrAB binds both ad

185 [a]pyrene-7,8-dihyrodiol-9,10-epoxide (BPDE)-adducted substrates.

186 removes potential steric overlap between the adducted template base and the incoming dCTP.

187 (ii) Poliota can accommodate a minor-groove-adducted template purine which adopts a syn conformation

188 AFB)G allowed full-length replication of the adducted template strand.

189 templates and the (-)-syn-trans-(C10R)-BPDE-adducted template, following replication with either the

190 ere by far the most frequent mutation on the adducted template, three- and six-base deletions were al

191 uctures of ternary hPol kappa complexes with adducted template-primer DNA reveal non-productive (dGTP

192 l three of the C10R and two of the C10S BPDE-adducted templates 1 base 5' and 1 base 3' to the damage

193 full-length products with all the C10S BPDE-adducted templates and the (-)-syn-trans-(C10R)-BPDE-add

194 However, the other two C10R BPDE-adducted templates failed to allow any primer extension,

195 extension abilities using the C10R and C10S adducted templates, their binding affinities were simila

196 y(262) terminated synthesis on styrene oxide-adducted templates.

197 Mental retardation, Aphasia, Shuffling gait, Adducted thumbs) syndrome.

198 SH (corpus callosum hypoplasia, retardation, adducted thumbs, spastic paraplegia, and hydrocephalus).

199 orpus callosum agenesis, mental retardation, adducted thumbs, spasticity, and hydrocephalus.

200 nuclease assembled at the site of a psoralen-adducted thymine.

201 otion was supported by the finding that LGE2 adducted to albumin at a rate that exceeded that of 4-hy

202 d by the finding that >60% of levuglandin E2 adducted to albumin within 20 s, whereas approximately 5

203 Oxidative metabolites of curcumin adducted to and inhibited the inhibitor of NF-kappaB kin

204 acterization of a family of surface proteins adducted to choline, regulation of virulence, and the re

205 s a methylphosphonic acid (MeP) product when adducted to human butyrylcholinesterase (BChE).

206 case and RQC domains of WRN protein with HNE adducted to Lys577 and Cys727 and provided a potential m

207 L-754,394 that had been adducted to P450 3A4 was hydrolyzed under the conditions

208 ns, whereas administered 18:1-NO2 is rapidly adducted to plasma thiol-containing proteins and glutath

209 example, the ADP/ATP translocase 1 was found adducted to the 2-alkenals, acrolein and 4-hydroxy-2-hex

210 The ratio of adducted to total BuChE nonapeptides was calculated for

211 h abortive ligation that produces chemically adducted, toxic 5'-adenylated (5'-AMP) DNA lesions.

212 of intermolecular disulfides, containing one adducted toxin per glutaredoxin but with elimination of

213 We performed molecular modelling of BPDE-adducted TP53 duplex sequences to determine the degree o

214 NH(2)-terminal lysyl-adducted truncated peptides with lengths of 22, 25, and

215 ECD) of doubly protonated and divalent metal-adducted tyrosine O-sulfated peptides without basic amin

216 ed at position 6256 of M13MB102 DNA, and the adducted viral genome was electroporated into repair-pro

217 Variation for the measurement of percent adducted was <12% for all characterized quality control

218 wobble pair in the corresponding duplex not adducted with AFB(1).

219 after immunization with mouse serum albumin adducted with carboxyethylpyrrole, a unique oxidation fr

220 y, followed by guanine, opposite the adenine adducted with either BPDE isomer.

221 much of the laminin 5 isolated is covalently adducted with laminin 6 (alpha3beta1gamma1) and a novel

222 ptide spanning amino acids 126-129 which was adducted with the reactive iminium species derived from

223 everal orders of magnitude: >50% of LGE2 had adducted within 20 s.