ライフサイエンスコーパス: adenylate kinase

1 G-actin and the open-to-closed transition of adenylate kinase.

2 state in the ligand-free form of the enzyme adenylate kinase.

3 ic contributions of dynamics to catalysis in adenylate kinase.

4 method with the conformational transition of adenylate kinase.

5 o locate and delineate an anesthetic site on adenylate kinase.

6 conserved sequence elements of p21(Ras) and adenylate kinase.

7 alkanol site so-defined spans two domains of adenylate kinase.

8 In particular, we focus on adenylate kinase.

9 action that was further enhanced by purified adenylate kinase.

10 pha-helices with overall topology similar to adenylate kinase.

11 that it folds in a manner similar to that of adenylate kinase.

12 ich is different from the situation found in adenylate kinase.

13 s to the location of the AMP-binding site in adenylate kinase.

14 this activity was purified and identified as adenylate kinase.

15 motion associated with substrate binding in adenylate kinase.

16 eport of NDP kinase activity associated with adenylate kinase.

17 ion 77, which is located in the main body of adenylate kinase.

18 ibutable to an extramitochondrial isoform of adenylate kinase.

19 adenosine-5'-pentaphosphate, an inhibitor of adenylate kinase.

20 the ABC transporter CFTR can function as an adenylate kinase.

21 irradiation could then trap the (32)P on the adenylate kinase.

22 is examined and compared with those of other adenylate kinases.

23 sequence and structural conservation in all adenylate kinases.

24 shown to share structural similarities with adenylate kinases.

25 utral cholesterol ester hydrolase 1 (Nceh1), adenylate kinase 1 (Ak1), inositol polyphosphate 5-phosp

26 ucleoside triphosphate diphosphohydrolase 5/ adenylate kinase 1/cytidine monophosphate kinase 1 axis

27 her with cytidine monophosphate kinase-1 and adenylate kinase-1, constitute an ATP hydrolysis cycle t

28 s such as glutamate dehydrogenase 2 (GLUD2), adenylate kinase 2 (AK2) and transketolase (TKT).

29 Here we find that adenylate kinase 2 (AK2), a mitochondrial enzyme that re

30 Adenylate kinase 2 (AK2), which balances adenine nucleot

31 Adenylate kinase 2 plays key roles in cellular energy an

32 ing two of high confidence, calreticulin and adenylate kinase 2.

33 sequence to follow the release of Smac, Omi, adenylate kinase-2, cytochrome c, and apoptosis-inducing

34 eous release of cytochrome c, Smac, Omi, and adenylate kinase-2.

35 In this study, we show that adenylate kinase-4 (AK4) is a progression-associated gen

36 nucleoside diphosphate kinase (Ndk), ATPase, adenylate kinase, 5'-nucleotidase, and ATP-modifying enz

37 nderlying conformational energy landscape of adenylate kinase, a crucial protein for signal transduct

38 The adenylate kinase active center probe P(1),P(5)-di(adenos

39 hypertrophy, the reduced creatine kinase and adenylate kinase activities limited energy delivery to t

40 elevant concentrations of AMP, CFTR exhibits adenylate kinase activity (ATP + AMP &lrarr2; 2 ADP).

41 We found that CFTR also has adenylate kinase activity (ATP + AMP <=> ADP + ADP) that

42 Because CFTR NBD2 has reversible adenylate kinase activity (ATP + AMP<==> ADP + ADP) that

43 ), CFTR Cl(-) channel function is coupled to adenylate kinase activity (ATP+AMP <==> 2 ADP).

44 Deficit in adenylate kinase activity abrogated AMP signal generatio

45 lier observation that mutations that disrupt adenylate kinase activity also disrupt ADP inhibition.

46 Finding that ADP inhibits function via an adenylate kinase activity also helps explain the earlier

47 The model also includes ecto-adenylate kinase activity and feed-forward inhibition of

48 studies suggest that HCV NS4B possesses both adenylate kinase activity and nucleotide hydrolase activ

49 o and in cell-free extracts, indicating that adenylate kinase activity by Mre11/Rad50 promotes DNA-DN

50 Inhibition of adenylate kinase activity diminished both actomyosin con

51 Swelling and the release of adenylate kinase activity have been determined simultane

52 ctomyosin system, which possesses endogenous adenylate kinase activity in both compartments, substrat

53 ssays demonstrated a comparable reduction in adenylate kinase activity in oda5 flagella, and also in

54 Pase activity in the presence of ATP and 2), adenylate kinase activity in the presence of ATP plus ph

55 tenance of chromosome (SMC) protein, exhibit adenylate kinase activity in the presence of physiologic

56 es cell death as evidenced by the release of adenylate kinase activity into the cell medium, with no

57 Furthermore, the results suggest that adenylate kinase activity is important for normal CFTR c

58 A CFTR gating mechanism model based on adenylate kinase activity is proposed.

59 no biochemical data demonstrating intrinsic adenylate kinase activity of a membrane-bound ABC transp

60 o suggest that a better understanding of the adenylate kinase activity of CFTR may be of value in dev

61 ly activates the ATPase activity but not the adenylate kinase activity of Fap7, identifying Rps14 as

62 of the conserved signature motif reduces the adenylate kinase activity of Rad50 but does not reduce A

63 Whereas previous work indicated that adenylate kinase activity regulated channel opening, our

64 to the hydrolysis of NTP and NDP substrates, adenylate kinase activity was detected in purified prepa

65 ve phosphotransfer mechanisms were explored; adenylate kinase activity was unaltered, and although GA

66 te photolabeling of the AMP-binding site and adenylate kinase activity were disrupted in Q1291F CFTR.

67 re maintenance in S. cerevisiae, correlating adenylate kinase activity with in vivo functions.

68 of current was attenuated when we prevented adenylate kinase activity with P1,P5-di(adenosine-5') pe

69 apparent human ortholog of Hbr1p, assays for adenylate kinase activity, autophosphorylation, and ATPa

70 We developed a biochemical assay for adenylate kinase activity, in which the radioactive gamm

71 At physiologic nucleotide concentrations, adenylate kinase activity, rather than ATPase activity m

72 he increase of ATP in Glu(-) cells is due to adenylate kinase activity, transforming AMP into ADP whi

73 roduction, a function lost in the absence of adenylate kinase activity.

74 mal subunit with an uncommon dual ATPase and adenylate kinase activity.

75 maintenance of chromosome protein, also have adenylate kinase activity.

76 h CFTR at ATP-binding site 2 is required for adenylate kinase activity.

77 that labeled CFTR, thereby demonstrating its adenylate kinase activity.

78 rase beta chain (rpoC [mhp635]) (P = 0.003), adenylate kinase (adk [mhp208]) (P = 0.001), prolyl amin

79 The enzyme adenylate kinase (ADK) features two substrate binding do

80 the closed-to-open transitions of the enzyme adenylate kinase (AdK) in its substrate-free form, we co

81 ion pathway of the phosphotransferase enzyme adenylate kinase (AdK) in the absence and presence of an

82 ssays that monitor the catalytic activity of adenylate kinase (ADK) in the equilibrium transphosphory

83 Adenylate kinase (AdK) is a phosphoryl-transfer enzyme w

84 Adenylate kinase (AdK), a phosphotransferase enzyme, pla

85 tability, and function of a selected enzyme, adenylate kinase (Adk), by monitoring changes in its enz

86 HOBr with three well-characterized proteins [adenylate kinase (ADK), ribose binding protein, and bovi

87 S17-L14-L24-L5-S14-S8-L6-L18-S5-L30-L15-SecY-adenylate kinase (Adk)-methionine aminopeptidase (Map)-i

88 lucosamine-6-phosphate deaminase (NagB), and adenylate kinase (Adk).

89 transition between open and closed states of adenylate kinase (ADK).

90 genetic relation to bacterial and eukaryotic adenylate kinases (ADK), it was concluded that the archa

91 esidues are conserved at the active sites of adenylate kinases (Adk), suggesting that Pnk and Adk are

92 Adenylate kinases (ADKs) from four closely related metha

93 The kinetics of creatine kinase (CK) and adenylate kinase (AK) activities were monitored in intac

94 phosphate kinase (Ndk), 5' nucleotidase, and adenylate kinase (Ak) activities.

95 en reported that NBD2 additionally possessed adenylate kinase (AK) activity.

96 at have nucleoside diphosphate kinase (Ndk), adenylate kinase (Ak) and 5'-nucleotidase activity, the

97 e characterized the conformational change of adenylate kinase (AK) between open and closed forms by c

98 K was 30-38% identical to the members of the adenylate kinase (AK) family while EhUK was more similar

99 We report evidence that adenylate kinase (AK) from Escherichia coli can be activ

100 yeast cell integrity by using the release of adenylate kinase (AK) into culture medium as a reporter

101 Adenylate kinase (AK) is a ubiquitous enzyme that regula

102 Construction of a Thermotoga neapolitana adenylate kinase (AK) library using PERMUTE revealed tha

103 ional fluctuations in the phosphotransferase adenylate kinase (AK) throughout its active reaction cyc

104 he method employs a four-enzyme system (PDE, adenylate kinase (AK) using excess CTP instead of ATP as

105 P, indicating redistribution of flux through adenylate kinase (AK), glycolytic and guanine nucleotide

106 In adenylate kinase (AK), this involves a large-amplitude r

107 In the enzyme adenylate kinase (AK), two small domains (LID and NMP) c

108 secretion was the suppression in the rate of adenylate kinase (AK)-catalyzed phosphorylation of AMP b

109 Adenylate kinase (AK)-catalyzed transfer of adenine nucl

110 scherichia coli with a temperature-sensitive adenylate kinase (AK).

111 the binding interaction of Escherichia coli adenylate kinase (AK).

112 Adenylate kinase (AK; ATP:AMP phosphotransferase, EC 2.7

113 We fused cytosolic adenylate kinase (AK1) and its isoform (AK1beta) with en

114 tional changes in the LID and NMP domains of adenylate kinase (AKE) are known to be key to ligand bin

115 the reversible conformational transition of Adenylate Kinase (AKE) between the open to the closed co

116 Adenylate kinase (AKe) from E. coli is a small, single-c

117 asured for two complexes of Escherichia coli adenylate kinase (AKe), viz., AKe.

118 Adenylate kinases (AKs) are phosphotransferases that reg

119 ed cardiomyocytes, introduction of exogenous adenylate kinase along with millimolar MgATP and AMP ind

120 Knockdown of adenylate kinase also failed to affect metformin stimula

121 induced by the ADP-generating substrates of adenylate kinase, AMP and MgATP, were indistinguishable

122 This phosphotransfer function renders adenylate kinase an important component for optimal myoc

123 Application to adenylate kinase, an allosteric enzyme composed of three

124 mong these genes, ADK1 and ADO1, encoding an adenylate kinase and an adenosine kinase, respectively,

125 ifs characteristic of adenylate kinases, and adenylate kinase and ATPase activities have been reporte

126 idating this procedure on simulated data for adenylate kinase and lactoferrin, we show how cryo-EM da

127 wns demonstrated direct interactions between adenylate kinase and several phage-coded enzymes, as wel

128 intermembrane space proteins: cytochrome c, adenylate kinase and sulfite oxidase.

129 e between the tryptophan of the F137W mutant adenylate kinase and the AEDANS-labeled Cys-77 decreased

130 ononucleotide binding proteins that includes adenylate kinase and the G-proteins.

131 he intermembranous proteins cytochrome c and adenylate kinase and the release from the matrix of sequ

132 d the corresponding amplitudes of motions in adenylate kinase and their linkage to catalytic function

133 s based on their conservation among archaeal adenylate kinases and mobility within the structures.

134 rovide specific knowledge about stability in adenylate kinases and more generally suggest that molecu

135 emonstrates the presence of 5'-nucleotidase, adenylate kinase, and a putative ATP reductase activity.

136 y phosphoryl fluxes through creatine kinase, adenylate kinase, and glycolysis in preconditioned heart

137 p exhibits sequence motifs characteristic of adenylate kinases, and adenylate kinase and ATPase activ

138 , the larger-scale motions in substrate-free adenylate kinase are not random, but preferentially foll

139 Importantly, the model identifies ecto-adenylate kinase as a key regulator of ASL ATP and propo

140 These results identify adenylate kinase as a specific component of the complex.

141 cking nucleoside diphosphate kinase, can use adenylate kinase as an alternative source of nucleoside

142 esent study identifies basal ADP content and adenylate kinase as key determinants of bioenergetics du

143 tructures have been studied in five enzymes: adenylate kinase, aspartate aminotransferase, citrate sy

144 Adenylate kinase, associated with AMP signaling, is a se

145 Adenylate kinase associates with the K(ATP) channel comp

146 systems of broad biological interest such as adenylate kinase, ATP-driven calcium pump SERCA, leucine

147 mational change pathway for Escherichia coli adenylate kinase based on two crystal structures, namely

148 enylate kinase is fairly efficient, but that adenylate kinase becomes rate-limiting for DNA synthesis

149 All three ABC adenylate kinases bind and hydrolyze ATP in the absence

150 In adenylate kinases, binding of the two ADP molecules is c

151 domain of an SMC protein in complex with the adenylate kinase bisubstrate inhibitor P(1),P(5)-di(aden

152 rally-related fragments of Bacillus subtilis adenylate kinase (BsAK) and Thermotoga neapolitana adeny

153 NBD1, human NBD1, and human NBD2 function as adenylate kinases but not as ATPases.

154 r between a mesophilic and hyperthermophilic adenylate kinase, but are strikingly similar at temperat

155 ytochrome c, as ceramides induced release of adenylate kinase, but not fumerase from isolated mitocho

156 n Escherichia coli ndk mutant, implying that adenylate kinase can meet a demand for deoxyribonucleosi

157 Adenylate kinase catalysis accelerates the transition fr

158 distinct, yet chemically related, ATPase and adenylate kinase catalytic activities that together orch

159 low creatine kinase activity, inhibition of adenylate kinase-catalyzed phosphotransfer abolished nuc

160 In pacing-induced failing heart, adenylate kinase-catalyzed phosphotransfer increased by

161 In intact myocardium, the net adenylate kinase-catalyzed phosphotransfer rate was 10%

162 this study, we examined the contribution of adenylate kinase-catalyzed phosphotransfer to myocardial

163 ibrium constants for the creatine kinase and adenylate kinase-catalyzed reactions, allows one to esti

164 lly influence their interaction with the ABC adenylate kinase CFTR.

165 When functioning as an adenylate kinase, CFTR showed positive cooperativity for

166 rther indicate that the active center of the adenylate kinase comprises ATP-binding site 2.

167 F(0) ATP synthase or the forward reaction of adenylate kinase could not fully account for the culture

168 he corresponding monophosphate by the use of adenylate kinase, creatine phosphate, and creatine kinas

169 P signal generation and reduced the vascular adenylate kinase/creatine kinase activity ratio essentia

170 ic concentrations of ADP and AMP were added, adenylate kinase-deficient Q1291F channels opened signif

171 e previously reported free energy surface of adenylate kinase, deformations along the first mode prod

172 n ABC transporter plays an important role in adenylate kinase-dependent CFTR gating.

173 idue in CFTR, Gln-1291, selectively disrupts adenylate kinase-dependent channel gating at physiologic

174 Additional studies suggested that adenylate kinase-dependent inhibition involved phosphotr

175 tive tissues, in which AMP is generated from adenylate kinase during states of high energy demand, th

176 We now demonstrate that an ecto-adenylate kinase (ecto-AK) contributes to the metabolism

177 , suggesting the involvement of cell surface adenylate kinase, F(1)F(0) ATP synthase, and nucleoside

178 cale atomic fluctuations in hinge regions of adenylate kinase facilitate the large-scale, slower lid

179 Thus, this study provides evidence that adenylate kinase facilitates the transfer of high-energy

180 not bind to the canonical ATP site found in adenylate kinase family members.

181 okinases (PRKs) are octameric members of the adenylate kinase family of enzymes.

182 domain that is a distinctive feature of the adenylate kinase family of proteins.

183 es of the enzymes are similar to that of the adenylate kinase from archaeal Sulfolobus acidocaldarius

184 Adenylate kinase from Escherichia coli (AK(eco)) populat

185 Adenylate kinase from Escherichia coli consists of three

186 to increase stability, in silico mutants of adenylate kinase from the mesophile Bacillus subtilis we

187 osed to be important in thermal stability of adenylate kinase from the thermophile Bacillus stearothe

188 The crystal structures of adenylate kinases from the psychrophile Bacillus globisp

189 The crystal structures of adenylate kinases from the thermophile Methanococcus the

190 Adenylate kinase function is critical because a rise in

191 thin a 1-2-cM interval between D9S60 and the adenylate kinase gene (AK1).

192 Deletion of the adenylate kinase gene compromised nucleotide exchange at

193 The adenylate kinase genes (adkA) were cloned from four clos

194 Comparison of shikimate kinase to adenylate kinase has led to the identification of an ade

195 Since S.acidocaldarius adenylate kinase has the invariant Lys residue as well a

196 Assigning a signal processing role to adenylate kinase identifies a phosphorelay mechanism ess

197 e bond, we succeeded in arresting the enzyme adenylate kinase in a closed high-energy conformation th

198 It resembles adenylate kinase in having a P-loop containing core stru

199 required for outer arm assembly and anchors adenylate kinase in proximity to the arm.

200 rmational dynamics of Thermotoga neapolitana adenylate kinase in the free form (TNAK) and inhibitor-b

201 An adenylate kinase inhibitor blocks Mre11/Rad50-dependent

202 osine-5')pentaphosphate (Ap(5)A), a specific adenylate kinases inhibitor, inhibited wild-type CFTR.

203 However, little is known about how an ABC adenylate kinase interacts with ATP and AMP when both ar

204 These results indicate that adenylate kinase is a naturally occurring component of s

205 ff, and suggests that the catalytic speed of adenylate kinase is an evolutionary driver for organisma

206 cale motions observed upon ligand binding to adenylate kinase is dominated by enzyme-substrate intera

207 ation of the Lys residue in S.acidocaldarius adenylate kinase is explained.

208 tation of the missing NDP kinase function by adenylate kinase is fairly efficient, but that adenylate

209 ging revealed that a previously unidentified adenylate kinase is reduced 35-50% in oda5 flagella.

210 the glucose transporter isoform 3 (Glut-3), adenylate kinase isoenzyme 3 (AK-3), and tissue factor,

211 Here, knock out of the major adenylate kinase isoform, AK1, disrupted the synchrony b

212 esent evidence suggesting that in the enzyme adenylate kinase large "hinge bending" motions closely r

213 we measured the conversion of ADP to AMP by adenylate kinase located in the intermembrane space.

214 age of the nail-patella locus to the ABO and adenylate kinase loci on human chromosome 9q34.

215 were prepared by covalently incorporating an adenylate kinase mutant, possessing two thiol groups, in

216 With deficient adenylate kinase, nucleoside diphosphate kinase, which s

217 Here we asked whether, by similar criteria, adenylate kinase of the host cell is also a specific com

218 Adenylate kinases participate in maintaining the homeost

219 AEW, and NaOCl treatments were identified as adenylate kinase, phosphoglycerate kinase, glyceraldehyd

220 AK1 gene deletion blunted vascular adenylate kinase phosphotransfer, compromised the contra

221 nnels to metabolic challenge is regulated by adenylate kinase phosphotransfer.

222 ctivity in both compartments, substrates for adenylate kinase promoted the rate and amplitude of acto

223 These data indicate that the adenylate kinase reaction at NBD2 contributed to the inh

224 from three organisms catalyze the reversible adenylate kinase reaction in vitro.

225 to the conserved Q-loop glutamine during the adenylate kinase reaction.

226 wo CFTR ATP-binding sites is involved in the adenylate kinase reaction.

227 for Rad50 that incorporates both ATPase and adenylate kinase reactions as critical activities that r

228 that these enzymes catalyze both ATPase and adenylate kinase reactions.

229 Cytochrome c release was accompanied by adenylate kinase release, was not associated with mitoch

230 sequences of P-type pumps and two conserved adenylate kinase sequences that coordinate Mg2+ and/or b

231 Inspection of the known structure of adenylate kinase shows that the side chains of these res

232 ycogenolysis, coupled to creatine kinase and adenylate kinase, simulated published experiments made w

233 he second system, studied in this report, is adenylate kinase (Sp-AK), which uses 2 ADP to make ATP +

234 wo different enzymatic reactions, ATPase and adenylate kinase, that share a common ATP binding site i

235 In all cases except adenylate kinase, the backbone of residues located in an

236 In adenylate kinase, the side-chain of a residue located di

237 onsidered with earlier studies on myosin and adenylate kinase, these studies also implicate a special

238 ate kinase (BsAK) and Thermotoga neapolitana adenylate kinase (TnAK) with identical modifications at

239 TP-inhibited state is based on the action of adenylate kinase to catalyze phosphoryl transfer between

240 ying thermoadaptation of enzyme catalysis in adenylate kinase using ancestral sequence reconstruction

241 iscrepancy is due to the reverse reaction of adenylate kinase utilizing AMP.

242 ucleoside monophosphate kinases tested, only adenylate kinase was found to have NDP kinase activity.

243 ighly homologous mesophilic and thermophilic adenylate kinases, we generated a series of chimeric enz

244 Based on data from non-ABC adenylate kinases, we hypothesized that ATP and AMP mutu

245 rements of the refolding of Escherichia coli adenylate kinase were analyzed.

246 Adenylate kinase, which catalyzes the reversible ATP-dep

247 design more stable variants of a mesophilic adenylate kinase with only the sequence information of o